RESUMO
Bisphenol A (BPA) is an endocrine disruptor to which animals and humans are highly exposed. Many reports have established a relationship between BPA exposure and breast cancer incidence, especially during critical periods of development. However, its effects on the immune response in testicular tumour growth have not yet been described. Thus, we wanted to analyse the effect of perinatal BPA exposure in pregnant female mice and the immune response modulation and tumour growth in an intratesticular cancer model in offspring male mice. Pregnant female mice were exposed to a dose of 250 mg/kg/day/body weight of BPA in their drinking water. In adulthood, male offspring underwent intrascrotal inoculation with 4T1 cancer cells. On day 21 after inoculation, mice were euthanised, and serum was obtained to measure BPA levels using HPLC coupled to mass spectrometry. The percentages of immune cell populations in peripheral lymph nodes (PLN), the spleen and tumours were evaluated by flow cytometry. In addition, the tumour expression of IL-10, TNF-α and TGF-ß was analysed by RT-PCR. Of note, we found detectable circulating levels of BPA in the offspring of mothers exposed to it while pregnant. Remarkably, BPA treatment promoted tumour growth by about 75% compared to mice coming from female mice that did not receive the compound. Perinatal exposure to BPA modulated the percentages of different immune cells in the spleen and PLN. In addition, the expression of inflammatory-related cytokines (IL-10 and TNF-α) in the tumours was significantly enhanced compared to control and vehicle groups. In conclusion, the perinatal BPA administration in pregnant female mice modulated different cellular and molecular immune components that resulted in outstanding testicular tumour size in male offspring.
Assuntos
Poluentes Ocupacionais do Ar/imunologia , Compostos Benzidrílicos/imunologia , Poluição Ambiental/efeitos adversos , Fenóis/imunologia , Neoplasias Testiculares/induzido quimicamente , Animais , Feminino , Humanos , Masculino , Exposição Materna , Camundongos , Camundongos Endogâmicos BALB C , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Fatores de RiscoRESUMO
Background: Sertolioma is a slow-growing, non-invasive, firm and nodular tumor, malignant in 10% to 22% of cases andwith low metastatic potential. Old age and cryptorchidism increase up to 26 times its chances of development and associates it with malignancy. Paraneoplastic syndrome, shown in 20% to 30% of the animals, is due to the aromatization oftestosterone or the direct production of estrogen by tumor cells, leading to signs of feminization and bone marrow aplasia.The objective of this article is to report a case of sertolioma in a dog with dermatological characteristic symptoms, butpresenting an unusual aggressive behavior, both completely reverted after castration.Case: A 9-year-old, uncastrated, aggressive and uncontrollable Canadian Husky dog was treated at the InstitutionalVeterinary Hospital with parapenial volume increase and generalized alopecia. A scrotal testis of reduced size and flaccid consistency and a mass in a parapenial region of 11 x 7.5 x 8 cm in diameter, with a cystic contour, adhered to theabdominal musculature and painless to palpation were detected. Cytology of the parapenial mass presented an imagecompatible with seminoma or sertolioma, and the preputial smear revealed a predominance of superficial cells. Ultrasoundexamination showed a heterogeneous inguinal mass, with expansive cystic area, compatible with mass in retained inguinaltestis. Therapeutic course consisted of bilateral orchiectomy. Ectopic testis was firm to the cut, had whitish to yellowishcoloration and was surrounded by a tunica containing 200 mL of serosanguinolent liquid. The histology of the mass revealed sertolioma-compatible cell characteristics, with cell proliferation circumvented by fibrous connective tissue formingpoorly delimited lobes, moderate polymorphism with elongated cells, arranged in a palisade at the periphery of the lobes,vacuolated eosinophilic cytoplasm and vesiculous...(AU)
Assuntos
Animais , Cães , Tumor de Células de Sertoli/etiologia , Tumor de Células de Sertoli/veterinária , Neoplasias Testiculares/etiologia , Neoplasias Testiculares/veterinária , Orquiectomia/veterinária , Alopecia/veterinária , AgressãoRESUMO
Background: Sertolioma is a slow-growing, non-invasive, firm and nodular tumor, malignant in 10% to 22% of cases andwith low metastatic potential. Old age and cryptorchidism increase up to 26 times its chances of development and associates it with malignancy. Paraneoplastic syndrome, shown in 20% to 30% of the animals, is due to the aromatization oftestosterone or the direct production of estrogen by tumor cells, leading to signs of feminization and bone marrow aplasia.The objective of this article is to report a case of sertolioma in a dog with dermatological characteristic symptoms, butpresenting an unusual aggressive behavior, both completely reverted after castration.Case: A 9-year-old, uncastrated, aggressive and uncontrollable Canadian Husky dog was treated at the InstitutionalVeterinary Hospital with parapenial volume increase and generalized alopecia. A scrotal testis of reduced size and flaccid consistency and a mass in a parapenial region of 11 x 7.5 x 8 cm in diameter, with a cystic contour, adhered to theabdominal musculature and painless to palpation were detected. Cytology of the parapenial mass presented an imagecompatible with seminoma or sertolioma, and the preputial smear revealed a predominance of superficial cells. Ultrasoundexamination showed a heterogeneous inguinal mass, with expansive cystic area, compatible with mass in retained inguinaltestis. Therapeutic course consisted of bilateral orchiectomy. Ectopic testis was firm to the cut, had whitish to yellowishcoloration and was surrounded by a tunica containing 200 mL of serosanguinolent liquid. The histology of the mass revealed sertolioma-compatible cell characteristics, with cell proliferation circumvented by fibrous connective tissue formingpoorly delimited lobes, moderate polymorphism with elongated cells, arranged in a palisade at the periphery of the lobes,vacuolated eosinophilic cytoplasm and vesiculous...
Assuntos
Animais , Cães , Neoplasias Testiculares/etiologia , Neoplasias Testiculares/veterinária , Tumor de Células de Sertoli/etiologia , Tumor de Células de Sertoli/veterinária , Agressão , Alopecia/veterinária , Orquiectomia/veterináriaRESUMO
Introducción: El síndrome H es una enfermedad genética extremadamente rara de compromiso multisistémico, el cual clínicamente puede ser reconocido de forma precoz, ofreciendo de manera oportuna un seguimiento, tratamiento específico y asesoramiento genético. Objetivo: Presentar un caso con características «típicas del síndrome H¼ para favorecer su identificación precoz. Caso clínico: Varón de 8 años de edad, evaluado por tumoraciones testiculares, lesiones dérmicas tipo hiperpigmentación con hipertricosis, retraso del lenguaje, talla baja, deformidades articulares, hipoacusia neurosensorial bilateral, anemia, hipergammaglobulinemia y alteraciones óseas. En los estudios histológicos de la piel y las masas testiculares se observó infiltración linfoplasmocitaria. El secuenciamiento del gen SLC29A3 detectó una mutación homocigota c.1087 C>T (p.Arg363Trp; rs387907067) concluyente con el síndrome H, la cual ha sido reportada previamente. Conclusiones: Este es el primer caso reportado en Latinoamérica del síndrome H, cuyas características descritas son parte del espectro clínico. El hallazgo clínico principal, que orienta al diagnóstico, es la hiperpigmentación acompañada de hipertricosis.
Introduction: H Syndrome is an extremely rare genetic disease, with a multisystemic character and which can be identified in early childhood, offering the opportunity of specific treatment and genetic counselling. Objective: To present a clinical case with "typical" characteristics of H Syndrome. Clinical case: The case is presented of an 8-year-old male patient who presented with testicular tumours and skin lesions characterised by hyperpigmentation with hypertrichosis, language delay, short stature, and joint deformities. He also presented with bilateral sensorineural hearing loss, anaemia, hypergammaglobulinaemia, and bone disorders. Histopathology studies of the skin and testicular masses reported lymphoplasmacytic infiltration. Sequencing analysis of gene SLC29A3 showed the homozygote mutation c.1087 C>T (p.Arg363Trp; rs387907067). Conclusions: These findings are consistent with H syndrome, and this is the first reported case in Latin America. The key to the diagnosis is the finding of hyperpigmentation with hypertrichosis.
Assuntos
Humanos , Masculino , Criança , Neoplasias Testiculares/genética , Hiperpigmentação/genética , Proteínas de Transporte de Nucleosídeos/genética , Hipertricose/genética , Síndrome , Neoplasias Testiculares/diagnóstico , Neoplasias Testiculares/patologia , Estatura/genética , Hiperpigmentação/diagnóstico , Hiperpigmentação/patologia , Perda Auditiva Neurossensorial/genética , Hipertricose/diagnóstico , Hipertricose/patologia , Transtornos do Desenvolvimento da Linguagem/genética , América Latina , MutaçãoRESUMO
INTRODUCTION: H Syndrome is an extremely rare genetic disease, with a multisystemic character and which can be identified in early childhood, offering the opportunity of specific treatment and genetic counselling. OBJECTIVE: To present a clinical case with "typical" characteristics of H Syndrome. CLINICAL CASE: The case is presented of an 8-year-old male patient who presented with testicular tumours and skin lesions characterised by hyperpigmentation with hypertrichosis, language delay, short stature, and joint deformities. He also presented with bilateral sensorineural hearing loss, anaemia, hypergammaglobulinaemia, and bone disorders. Histopathology studies of the skin and testicular masses reported lymphoplasmacytic infiltration. Sequencing analysis of gene SLC29A3 showed the homozygote mutation c.1087 C>T (p.Arg363Trp; rs387907067). CONCLUSIONS: These findings are consistent with H syndrome, and this is the first reported case in Latin America. The key to the diagnosis is the finding of hyperpigmentation with hypertrichosis.