RESUMO
Protein bioaccessibility is a major concern in sorghum (Sorghum bicolor L. Moench) due to potential interactions with tannins affecting its nutritional value. Technological treatments such as boiling or alkaline cooking have been proposed to address this problem by reducing tannin-protein interactions. This research aimed to evaluate the impact of nixtamalization in the protein bioaccessibility from two sorghum varieties (red and white sorghum) during in vitro gastrointestinal digestion. Nixtamalization increased protein bioaccessibility in the non-digestible fraction (NDF) (5.26 and 26.31% for red and white sorghum, respectively). However, cooking showed a higher permeation speed of protein from red sorghum flours at the end of the intestinal incubation (9.42%). The SDS-PAGE profile of the digested fraction (DF) at 90 min of intestinal incubation indicated that, for red sorghum, cooking allows the formation of α and γ-kafirins while nixtamalization increase α-kafirin release. Principal Components Analysis (PCA) showed the association between nixtamalization and dissociation of δα kafirin complexes and increased protein content in the digestible fraction. In silico interactions indicated the highest biding energies for (+)-catechin and kafirin fractions (ß-kafirin: -7.0 kcal/mol; γ-kafirin: -5.8 kcal/mol, and δ-kafirin: -6.8 kcal/mol), suggesting a minor influence of depolymerized proanthocyanidin fractions with sorghum proteins as a result of the nixtamalization process. In conclusion, nixtamalization increased the bioaccessibility of sorghum proteins, depolymerizing condensed tannins, and breaking protein-tannin complexes. Such technological process improves the nutrimental value of sorghum, supporting its inclusion in the human diet.
Assuntos
Sorghum , Digestão , Grão Comestível , Farinha/análise , Humanos , TaninosRESUMO
Colorimetric nanosensors formed of polydiacetylene (PDA), triblock copolymer (L64 or F68), and sodium dodecyl sulfate (SDS), so-called nanoblends, were developed to detect enrofloxacin (ENRO) in aqueous media. The nanosensors show hydrodynamic diameter ranging from 234.2⯱â¯3.5 to 801.6⯱â¯17.8â¯nm for SDS concentrations of 13.0-21.0â¯mM, respectively. The lowest limit of detection was 0.054⯵M, which is five times smaller than the maximum limit allowed by the European Union. The response surfaces showed that both the SDS and ENRO concentrations influenced the colorimetric response (pâ¯<â¯0.05), and kinetic rate of colorimetric transition (RCT). SDS concentration between 11.0 and 14.0â¯mM in the nanoblend yielded the most sensitive nanosensors for detecting ENRO. When L64 was replaced by F68, the colorimetric response of the nanoblends was similar, but PDA/F68/SDS showed a slower RCT than PDA/L64/SDS. The developed nanosensor is a sensitive and simple device for the fast detection of ENRO.
Assuntos
Colorimetria , Enrofloxacina/análise , Polímero Poliacetilênico/química , Polímeros/química , Tensoativos/química , Antibacterianos/análise , Limite de Detecção , Dodecilsulfato de Sódio/químicaRESUMO
The clinical use of paclitaxel as a chemotherapeutic agent is limited by the severe acute and chronic hypersensitivity caused when it is administered via intraperitoneal or intravenous routes. Thus far, evidence has suggested that transient receptor potential vanilloid-1 (TRPV1) has a key role in the chronic neuropathy induced by paclitaxel. Despite this, the role of TRPV1 in paclitaxel -related acute nociception, especially the development of visceral nociception, has not been evaluated. Thus, the goal of this study was to evaluate the participation of TRPV1 in a model of acute nociception induced by paclitaxel in rats and mice. A single intraperitoneal (i.p.) paclitaxel administration (1â¯mg/kg, i.p.) produced an immediate visceral nociception response 1â¯h after administration, caused mechanical and heat hypersensitivity, and diminished burrowing behaviour 24â¯h after administration. These nociceptive responses were reduced by SB-366791 treatment (0.5â¯mg/kg, i.p., a TRPV1 antagonist). In addition, TRPV1-positive sensory fibre ablation (using resiniferatoxin, 200⯵g/kg, s.c.) reduced visceral nociception and mechanical or heat hypersensitivity caused by paclitaxel injection. Similarly, TRPV1 deficient mice showed a pronounced reduction in mechanical allodynia to paclitaxel acute injection and did not develop heat hypersensitivity. Moreover, 24â¯h after its injection, paclitaxel induced chemical hypersensitivity to capsaicin (a TRPV1 agonist, 0.01 nmol/site) and increased TRPV1 immunoreactivity in the dorsal root ganglion and sciatic nerve. In conclusion, TRPV1 is involved in mechanical and heat hypersensitivity and spontaneous-pain behaviour induced 24â¯h after a single paclitaxel injection. This receptor is also involved in visceral nociception induced immediately after paclitaxel administration.