RESUMO
Canine leproid granuloma (CLG) is a chronic form of dermatitis that has been associated with nontuberculous mycobacterial infections in Africa, Oceania, the Americas, and Europe. We report here a case of CLG associated with a member of the Mycobacterium tuberculosis complex (MTBC), which could be of public health concern. An 8-y-old pet dog developed 0.5-1-cm diameter, raised, firm, nonpruritic, alopecic, painless skin nodules on the external aspects of both pinnae. Histologic examination revealed severe pyogranulomatous dermatitis with intracellular Ziehl-Neelsen-positive bacilli that were immunoreactive by immunohistochemistry using a polyclonal primary antibody that recognizes tuberculous and nontuberculous Mycobacterium species. DNA extracted from formalin-fixed, paraffin-embedded skin sections was tested by a Mycobacterium genus-specific nested PCR assay targeting the 16S rRNA gene. BLAST sequence analysis of 214-bp and 178-bp amplicons showed 99.5% identity with members of the MTBC; however, the agent could not be identified at the species level. Although CLG has been associated traditionally with nontuberculous mycobacterial infections, the role of Mycobacterium spp. within the MTBC as a cause of this condition, and the role of dogs with CLG as possible sources of MTBC to other animals and humans, should not be disregarded given its zoonotic potential.
Assuntos
Dermatite , Infecções por Mycobacterium , Mycobacterium tuberculosis , Tuberculose , Humanos , Cães , Animais , Infecções por Mycobacterium/microbiologia , Infecções por Mycobacterium/veterinária , Mycobacterium tuberculosis/genética , RNA Ribossômico 16S/genética , Tuberculose/veterinária , Tuberculose/diagnóstico , Granuloma/veterinária , Granuloma/microbiologia , Dermatite/veterináriaRESUMO
Our study describes the clinical, epidemiologic, pathologic, immunohistochemical, and molecular aspects PCR of a case of cutaneous pythiosis in a donkey (Equus asinus) from Brazil. During a dry period, the animal grazed for 4 months around a pond where the vegetation remained green. Skin lesions were nodular, multifocal, and disseminated, mainly involving the legs, ventral chest, and mammary gland. On cut surface, there were multifocal to coalescent discrete yellow foci, and occasional small cavitations with a few kunkers. Ulcerative nodular pyogranulomatous and eosinophilic dermatitis with folliculitis and furunculosis were observed histologically. Hyphae were observed in sections stained with Gomori methenamine silver. Immunohistochemistry with Pythium insidiosum antibodies yielded strong immunostaining of hyphae. P. insidiosum DNA was extracted from tissues in paraffin blocks by amplification of a fragment of 105 bp, which targets the 5.8S ribosomal gene. After the diagnosis of pythiosis, the larger skin lesions were excised and treated as second intention healing wounds, which were completely healed 30 days after resection. Small skin lesions regressed spontaneously in ~60 days. The granulomatous inflammation and outcome of the disease in this donkey were similar to cases of pythiosis in cattle.
Assuntos
Equidae , Pitiose/diagnóstico , Pythium/isolamento & purificação , Dermatopatias Parasitárias/veterinária , Animais , Brasil , Inflamação/diagnóstico , Inflamação/parasitologia , Inflamação/terapia , Inflamação/veterinária , Pitiose/parasitologia , Pitiose/terapia , Pythium/genética , Análise de Sequência de DNA/veterinária , Dermatopatias Parasitárias/diagnóstico , Dermatopatias Parasitárias/parasitologia , Dermatopatias Parasitárias/terapiaRESUMO
A peritonite infecciosa felina (PIF) é uma enfermidade causada pelo coronavírus felino (CoVF) mutante,capaz de promover viremia persistente e resposta imunológica nociva. A PIF é caracterizada por inícioinsidioso, febre não responsiva persistente, depressão, inapetência, letargia, reação piogranulomatosaem tecidos, acúmulo de exsudatos nas cavidades corporais e alta mortalidade. Diagnóstico e tratamentoda PIF constituem desafios para o Médico Veterinário. Neste trabalho são revisados os principais aspectosrelativos ao diagnóstico e tratamento da PIF.
The feline infectious peritonitis (FIP) is a highly fatal disease caused by mutated form of feline entericcoronavirus, which promote persistent viraemia and immune response to harmful animal. The FIP is characterizedby insidious onset, persistent nonresponsive fever, depression, lack of appetite, lethargy, pyogranulomatoustissues reaction, accumulation of exudative effusions in body cavities and high mortality. Thediagnosis and treatment of FIP remains a challenge for practicing veterinarians. In this paper we review themain aspects related to the diagnosis and treatment of FIP.
Assuntos
Animais , Gatos , Doenças Transmissíveis/veterinária , Peritonite Infecciosa Felina/diagnóstico , Coronavirus FelinoRESUMO
A peritonite infecciosa felina (PIF) é uma enfermidade causada pelo coronavírus felino (CoVF) mutante,capaz de promover viremia persistente e resposta imunológica nociva. A PIF é caracterizada por inícioinsidioso, febre não responsiva persistente, depressão, inapetência, letargia, reação piogranulomatosaem tecidos, acúmulo de exsudatos nas cavidades corporais e alta mortalidade. Diagnóstico e tratamentoda PIF constituem desafios para o Médico Veterinário. Neste trabalho são revisados os principais aspectosrelativos ao diagnóstico e tratamento da PIF.(AU)
The feline infectious peritonitis (FIP) is a highly fatal disease caused by mutated form of feline entericcoronavirus, which promote persistent viraemia and immune response to harmful animal. The FIP is characterizedby insidious onset, persistent nonresponsive fever, depression, lack of appetite, lethargy, pyogranulomatoustissues reaction, accumulation of exudative effusions in body cavities and high mortality. Thediagnosis and treatment of FIP remains a challenge for practicing veterinarians. In this paper we review themain aspects related to the diagnosis and treatment of FIP.(AU)