RESUMO

Hydrosoluble vitamins B1, B2, B6 and PP are essential organic substances for human organism, functioning as coenzymes on several metabolic cycles. In the present investigation four vitamins of B complex and its vitamers contents were determined in royal jelly samples marketed in São Paulo, Brazil. A single extraction process was employed, and each vitamin was determined by HPLC using C18 column and detected by fluorescence. Four samples from different suppliers were analyzed, and the results varied from 0.08 to 0.41 mg/100g (vitamin B1 orthiamine); from 0.01 to 0.05 mg/100g (vitamin B2 or ribofl avin); from 0.13 to 0.38 mg/100g (piridoxal - vitamin B6); from 0.26 to 1.38 mg/100g (piridoxamine - vitamin B6); from 0.21 to 0.57 mg/100g (niacin - vitamin PP);and from 1.56 to 2.00 mg/100g (niacinamide - vitamin PP). These data show that royal jelly is not an important source of the analyzed vitamins, though the results indicate that the employed technique is suitable for determining these four vitamins and its vitamers.

Assuntos

RESUMO

Hydrosoluble vitamins B1, B2, B6 and PP are essential organic substances for human organism, functioning as coenzymes on several metabolic cycles. In the present investigation four vitamins of B complex and its vitamers contents were determined in royal jelly samples marketed in São Paulo, Brazil. A single extraction process was employed, and each vitamin was determined by HPLC using C18 column and detected by fluorescence. Four samples from different suppliers were analyzed, and the results varied from 0.08 to 0.41 mg/100g (vitamin B1 or thiamine); from 0.01 to 0.05 mg/100g (vitamin B2 or riboflavin); from 0.13 to 0.38 mg/100g (piridoxal - vitaminB6); from 0.26 to 1.38 mg/100g (piridoxamine - vitamin B6); from 0.21 to 0.57 mg/100g (niacin - vitamin PP);and from 1.56 to 2.00 mg/100g (niacinamide - vitamin PP). These data show that royal jelly is not an important source of the analyzed vitamins, though the results indicate that the employed technique is suitable for determining these four vitamins and its vitamers.

As vitaminas hidrossolúveis B1, B2, B6 e PP são importantes substâncias para o organismo humano, que atuam como coenzimas em diversos ciclos metabólicos. No presente trabalho, foram determinados os teores de quatro vitaminas do complexo B e de seus vitâmeros em amostras de geleia real comercializadas no Estado de São Paulo. Foi utilizado um único processo de extração e a determinação de cada vitamina foi realizada por CLAE utilizando-se coluna C18 e a detecção foi feita por fluorescência. Quatro amostras de diferentes fornecedores foram analisadas e os resultados variaram de 0,08 a 0,41 mg/100g (vitamina B1 ou tiamina); de 0,01 a 0,05 mg/100g (vitaminaB2 ou riboflavina); de 0,13 a 0,38 mg/100g (piridoxal vitamina B6); de 0,26 a 1,38 mg/100g (piridoxamina -vitamina B6); de 0,21 a 0,57 mg/100g (niacina vitamina PP) e de 1,56 a 2,00 mg/100g (niacinamida vitamina PP). Conclui-se que a geleia real não é fonte importante dessas vitaminas, mas verifica-se que a técnica utilizada é adequada para efetuar as determinações das quatro vitaminas analisadas e de seus vitâmeros.