RESUMO
Enhancing the initial stages of plant growth by using polymeric gels for seed priming presents a significant challenge. This study aimed to investigate a microgel derived from polyetheramine-poly(propylene oxide) (PPO) and a bisepoxide (referred to as micro-PPO) as a promising alternative to optimize the seed germination process. The micro-PPO integrated with an iron micronutrient showed a positive impact on seed germination compared with control (Fe solutions) in which the root length yield improved up to 39%. Therefore, the element map by synchrotron-based X-ray fluorescence shows that the Fe intensities in the seed primers with the micro-PPO-Fe gel are about 3-fold higher than those in the control group, leading to a gradual distribution of Fe species through most internal embryo tissues. The use of micro-PPO for seed priming underscores their potential for industrial applications due to the nontoxicity results in zebrafish assays and environmentally friendly synthesis of the water-dispersible monomers employed.
Assuntos
Aminas , Cucumis sativus , Germinação , Ferro , Microgéis , Sementes , Germinação/efeitos dos fármacos , Sementes/química , Sementes/metabolismo , Sementes/crescimento & desenvolvimento , Sementes/efeitos dos fármacos , Cucumis sativus/metabolismo , Cucumis sativus/crescimento & desenvolvimento , Cucumis sativus/química , Ferro/metabolismo , Ferro/química , Aminas/química , Aminas/metabolismo , Microgéis/química , Compostos de Epóxi/química , Compostos de Epóxi/metabolismo , Peixe-Zebra/metabolismo , AnimaisRESUMO
Spent mushroom substrate (SMS) is a promising alternative for supplementing oyster mushroom substrate, replacing conventional cereal bran. Therefore, the objective was to evaluate the production of Pleurotus ostreatus supplemented with Lentinula edodes' SMS, through the nutritional analysis of the substrate. Wheat straw was used as substrate and supplemented with rice bran (RB) or SMS in 0%, 7%,15% and 30%. Ca, K, Mg, Mn, Zn, Cu and Fe contents of the cultivation substrates (before and after harvest) were determined through atomic absorption spectrophotometry. Mycelial growth (cm²/day), mycelial time colonization (days), number of clusters, number of pileus, average clusters weight (g), pileus lenght (cm) and width (cm), productivity (1st, 2nd and 3rd flush) (%), biological efficiency (%) of mushrooms were evaluated. Results indicated mycelial growth was higher (0.87 cm²/day compared to the Control) when the substrate was supplemented regardless of the source. The proportions of 15% of SMS achieved the highest biological efficiency (107% - 15% SMS versus 66% - Control). The only nutrients that showed differences in absorption were Ca, K and Mn, in which substrates supplemented with SMS had greater absorption of Ca (5.37 g.kg- 1 versus 1.94 g.kg- 1 in Control) while substrates supplemented with RB absorbed more K (6.56 g.kg- 1 versus 3.74 g.kg- 1 in Control). The mineral composition of the substrate has a direct impact on the growth and yield of P. ostreatus, highlighting the potential of SMS as a alternative to traditional bran supplementation.
Assuntos
Agaricales , Pleurotus , Triticum , Nutrientes , Suplementos NutricionaisRESUMO
Phosphorus (P) availability is important for metabolic process, tillering and formation of a vigorous root system in sugarcane, but sugarcane varieties differ in P uptake efficiency. This study evaluated the enzymatic, nutritional, and biometric parameters of two sugarcane varieties under two conditions of P availability by monitoring the initial development of plants grown in nutrient solution. The experiment was performed using randomized complete block design (RCBD) with five replicates and included two varieties, RB966928 (high nutritional requirements) and RB867515 (low nutritional requirements), and two concentrations of P in the nutrient solution: low (2 mg L-1) and suitable (16 mg L-1). Carbohydrate concentrations and partitioning, leaf nutrient concentrations, enzymatic activity, and shoot and root biometric parameters were analyzed. Regardless of sugarcane variety and the part of the plant, reducing sugar were approximately 32.5% higher in RB867515 and 38.5% higher in RB966928 under suitable P compared with low P. Sucrose concentrations were significantly higher in both varieties under suitable P than in low P. According to PCA, the relationship between reducing sugars and sucrose was closer in RB966928 than in RB867515. Under low P, soluble protein content decreased, and the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) and the concentrations of hydrogen peroxide (H2O2), and malondialdehyde (MDA). The variety RB966928 under suitable P appears to have a high capacity for proline (120%) upregulation under abiotic stress compared with RB867515 (54%), and thus higher biomass accumulation of this RB966928 variety; however, RB867515 had superior results compared to RB966928 under low P. Suitable P increased leaf concentrations of N, P, Mg, B, and Mg and decreased leaf Zn content. Root and shoot dry matter, root length, plant height, and root and stalk diameter increased by suitable P. Regardless of variety, both nutritional and biometric parameters were directly influenced by P levels, including sugarcane yield. In relation of sugarcane dry matter, RB966928 was less sensitive to low P levels and more responsive to P supply than RB867515 and thus may be more suitable for environments in which P is limiting.
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Studying the composition of a certain food is not enough to predict its health benefits. Research over the past decades has decisively strengthened the notion that any putative health benefit is best related to the fraction of compounds transferred from ingested foods into the body since the absorption may be incomplete after oral consumption. In other words, the bioavailability of food components is crucial information. Therefore, a variety of in vitro models have been developed to predict their bioaccessibility and bioavailability in the most diverse food matrices and food products. These models can also be applied to study the impact of several endogenous or exogenous factors on the bioaccessibility and bioavailability of nutrients and bioactive compounds, guiding nutrition and food scientists, technologists, and engineers towards the development of strategies to optimize the positive impact of the diet on well-being and quality of life. While bioavailability is ideally examined in human volunteers, in vitro digestion methods, as well as intestinal absorption and microphysiological models, simulate human physiological conditions. Additionally, in vitro methods are alternatives to offset ethical, economical, and experimental limitations associated with in vivo studies conducted either with individuals or animals. This graphical review draws parallels between in vitro models mimicking digestion processes, uptake, absorption, metabolism, and distribution of dietary compounds and human physiology.
RESUMO
The effect of in ovo threonine (Thr) supplementation on the ileal expression of glucose, peptide and amino acid transporters was assessed in Salmonella Enteritidis-challenged broiler chicks. At 17.5 days of incubation, fertile eggs were supplemented in the amniotic fluid with sterile saline or 3.5% threonine. Hatchlings were individually weighed, and Salmonella Enteritidis negative status was confirmed. At 2 days of age, half of the birds of each group were inoculated with sterile nutrient broth or Salmonella Enteritidis inoculum. Relative expression of sodium-dependent glucose transporter 1 (SGLT1), glucose transporter 2 (GLUT2), di- and tri-peptide transporter 1 (PepT1) and alanine, serine, cysteine, threonine transporter (ASCT1) was assessed at hatch, 2 and 9 days of age, i.e., before inoculation and 7 days post-inoculation (dpi). At 9 days of age (7dpi), threonine increased SGLT1 and GLUT2 expression, whereas GLUT2 expression decreased in Salmonella-challenged birds. There was a significant interaction between threonine and Salmonella for PepT1 and ASCT1. Threonine increased PepT1 expression only in non-challenged birds. In addition, in ovo supplementation increased expression of ASCT1 regardless of post-hatch inoculation; Salmonella inoculation resulted in decreased expression of ASCT1 only in supplemented birds. The results suggest that while intra-amniotic threonine administration in broiler embryos increases the expression of genes related to the absorption of monosaccharides and amino acids, Salmonella challenge may negatively affect the expression of protein related transporters in the ileum of broilers.
Assuntos
Doenças das Aves Domésticas , Salmonella enteritidis , Animais , Galinhas/metabolismo , Suplementos Nutricionais , Expressão Gênica , Íleo/metabolismo , Nutrientes , Óvulo , Doenças das Aves Domésticas/metabolismo , Treonina/farmacologiaRESUMO
It is important to consider the nutritional demand among genotypes of the same species to achieve high yields. Thus, the objective of this study was to determine the concentration of nutrients in leaves, flowers, grains, and fruit straw in conilon coffee genotypes. The experiment was carried out under a randomized block design, with three replications and the evaluation of nine genotypes. Leaf collections were carried out every three months, from August 2019 to May 2020. Flowers were collected at flowering in July 2019 and fruits in June 2020, upon complete maturation of the genotypes. The materials were dried in an oven and sent for laboratory analysis to determine the nutritional content. Data were subjected to analysis of variance followed by a comparison of means and estimates of genetic parameters and clustering using the hierarchical method (UPGMA). The nutrients found in the highest concentrations in the evaluated plant organs were N and K for macronutrients and Fe for micronutrients. For the leaves, the concentrations of the main nutrients were high in the first and reduced in the last evaluated periods, possibly due to mobilization to the fruits. Considering all the plant tissues evaluated, the order of concentration of macronutrients and micronutrients was N > K > Ca > P = Mg = S and Fe > B > Mn > Cu > Zn, respectively. For a nutritional diagnosis, it is important to take comparisons of the genetic diversity and evaluation periods into consideration.
RESUMO
This work deepens our understanding of starch digestion and the consequent absorption of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were digested with α-amylase in an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process. This study innovates with respect to the existing literature, because it considers the impact of simultaneous digestion and absorption processes occurring during the intestinal digestion of starchy foods and adopts phenomenological models that deal in a more realistic manner with the behavior found in the small intestine. Operating the i-IDS at different flow/dialysate flow ratios resulted in distinct generation and transfer curves of reducing sugars mass. This indicates that the operating conditions affected the mass transfer by diffusion and convection. However, the transfer process was also affected by membrane fouling, a dynamic phenomenon that occurred in the i-IDS. The experimental results were extrapolated to the human small intestine, where the times reached to transfer the hydrolytic products ranged between 30 and 64 min, according to the flow ratio used. We consider that the i-IDS is a versatile system that can be used for assessing and/or comparing digestion and absorption behaviors of different starch-based food matrices as found in the human small intestine, but the formation and interpretation of membrane fouling requires further studies for a better understanding at physiological level. In addition, further studies with the i-IDS are required if food matrices based on fat, proteins or more complex carbohydrates are of interest for testing. Moreover, a next improvement step of the i-IDS must include the simulation of some physiological events (e.g., electrolytes addition, enzyme activities, bile, dilution and pH) occurring in the human small intestine, in order to improve the comparison with in vivo data.
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Abstract Background: Kaolin may improve quail performance by maintaining intestinal integrity and improving nutrient absorption. Objective: To evaluate the overall performance, egg quality, moisture, nitrogen and calcium content in the excreta, intestinal morphology and financial analysis of kaolin in Japanese quail feed during the production phase. Methods: A total of 192 Japanese quails, distributed in a completely randomized design with four treatments and six repetitions with eight birds each, were used. Kaolin levels added to the diet were 0, 1.5, 3.0, and 4.5%. Results: Kaolin reduced feed consumption. Additionally, egg production, feed conversion and viability increased. Moisture, nitrogen and calcium in the excreta decreased. Height and width of the intestinal villi increased with increasing levels of kaolin in the diet. Kaolin had no effect on egg quality. Inclusion level of 1.5% kaolin allowed for greater economic gains. Conclusion: Kaolin improved performance, intestinal morphology, and reduced the moisture, nitrogen and calcium contents in the excreta. Egg quality remained unchanged with the addition of kaolin, and the best economic response resulted with the addition 1.5% kaolin.
Resumen Antecedentes: El caolín puede mejorar el rendimiento de la codorniz al mantener la integridad intestinal y una mayor absorción de nutrientes. Objetivo: Evaluar el rendimiento general, calidad del huevo, humedad, contenido de nitrógeno y calcio en la excreta, morfología intestinal, y realizar un análisis financiero de la inclusion de caolín en el alimento de codorniz japonesa durante la fase de producción. Métodos: Se utilizaron un total de 192 codornices japonesas, distribuidas en un diseño completamente al azar con cuatro tratamientos y seis repeticiones con ocho aves cada una. Los niveles de caolín agregado a la dieta fueron 0; 1,5; 3,0; y 4,5%. Resultados: El caolin redujo el consumo de alimento, hubo una mejora en el porcentaje de huevos producidos, la conversión del alimento y la viabilidad, Los niveles de humedad, nitrógeno y calcio en la excreta disminuyeron, la altura y el ancho de las vellosidades intestinales aumentaron con la inclusión de niveles crecientes de caolín. No hubo efecto sobre la calidad del huevo. Los niveles de inclusión de 1,5% de caolín permitieron mayores ganancias económicas. Conclusión: El caolín mejoró el rendimiento, la morfología intestinal y redujo los contenidos de humedad, nitrógeno y calcio en las excretas. La calidad de los huevos producidos se mantuvo sin cambios con la adición de diferentes niveles de caolín, y el mejor nivel de inclusion de caolín -según el análisis económico- fue de 1,5%.
Resumo Antecedentes: O caulim pode melhorar o desempenho de codornas mantendo a integridade intestinal e maior absorção de nutrientes. Objetivo: Avaliar o desempenho global, a qualidade do ovo, a umidade, o teor de nitrogênio e cálcio na excreta, a morfologia intestinal e a análise financeira do caulim em codornas japonesas durante a fase de produção. Métodos: Foram utilizadas 192 codornas japonesas, distribuídas em delineamento inteiramente casualizado, com quatro tratamentos e seis repetições, com oito aves cada. Os níveis de caulim adicionados à dieta foram 0; 1,5; 3,0; 4,5%. Resultados: O consumo de ração foi reduzido, houve melhora na porcentagem de ovos produzidos, conversão alimentar e viabilidade, os níveis de umidade, nitrogênio e cálcio nas excretas diminuíram, a altura e a largura das vilosidades intestinais aumentaram com a inclusão de níveis crescentes de caulim. Não houve efeito na qualidade do ovo. Os níveis de inclusão de 1,5% de caulim permitiram maiores ganhos econômicos. Conclusão: O caulim melhorou o desempenho, a morfologia intestinal e reduziu os teores de umidade, nitrogênio e cálcio na excreta. A qualidade dos ovos produzidos permaneceu inalterada com a adição de diferentes níveis de caulim, e o melhor nível de caulim, de acordo com a análise econômica, foi de 1,5% como aditivo para as codornas japonesas.
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Flying mammals present unique intestinal adaptations, such as lower intestinal surface area than nonflying mammals, and they compensate for this with higher paracellular absorption of glucose. There is no consensus about the mechanistic bases for this physiological phenomenon. The surface area of the small intestine is a key determinant of the absorptive capacity by both the transcellular and the paracellular pathways; thus, information about intestinal surface area and micro-anatomical structure can help explain differences among species in absorptive capacity. In order to elucidate a possible mechanism for the high paracellular nutrient absorption in bats, we performed a comparative analysis of intestinal villi architecture and enterocyte size and number in microchiropterans and rodents. We collected data from intestines of six bat species and five rodent species using hematoxylin and eosin staining and histological measurements. For the analysis we added measurements from published studies employing similar methodology, making in total a comparison of nine species each of rodents and bats. Bats presented shorter intestines than rodents. After correction for body size differences, bats had ~41% less nominal surface area (NSA) than rodents. Villous enhancement of surface area (SEF) was ~64% greater in bats than in rodents, mainly because of longer villi and a greater density of villi in bat intestines. Both taxa exhibited similar enterocyte diameter. Bats exceeded rodents by ~103% in enterocyte density per cm2 NSA, but they do not significantly differ in total number of enterocytes per whole animal. In addition, there is a correlation between SEF and clearance per cm2 NSA of L-arabinose, a nonactively transported paracellular probe. We infer that an increased enterocyte density per cm2 NSA corresponds to increased density of tight junctions per cm2 NSA, which provides a partial mechanistic explanation for understanding the high paracellular absorption observed in bats compared to nonflying mammals.
Assuntos
Quirópteros/anatomia & histologia , Quirópteros/fisiologia , Absorção Intestinal , Intestinos/anatomia & histologia , Intestinos/fisiologia , Roedores/anatomia & histologia , Roedores/fisiologia , Animais , Arabinose/metabolismo , Peso Corporal , Dieta , Enterócitos/metabolismo , Intestino Delgado/anatomia & histologia , Intestino Delgado/fisiologiaRESUMO
The effects of in ovo feeding with threonine (Thr) on intestinal morphology, ileal gene expression and performance of broiler chicken between 1 and 21 d of age (d) were assessed. On day 17.5 of incubation, fertile eggs were randomly allotted to 5 treatments of Thr injection in the amniotic fluid (0; 1.75; 3.5; 5.25; 7%, corresponding to 17.5; 35; 52.5 and 70 mg Thr/mL). After hatch, chicks were given a commercial corn-soybean diet up to 21 d. Daily feed intake (FI), body weight (BW), and food conversion ratio (FCR) were measured from 1 to 7, 14, and 21 d of age. The ileal gene expression of mucin (MUC2), peptide transporter (PepT1), and aminopeptidase enzyme (APN) were evaluated on day of hatch and at 21 d, as well as intestinal morphometric traits. In ovo feeding with threonine significantly increased final weight (FI) and weight gain (WG) and decreased FCR in the period from 1 to 21 d. Threonine levels affected beneficially the villus height, vilo: crypt ratio and villus area on day of hatch and at 21 d. At hatch, all Thr levels increased the expression of MUC2 and PepT1 compared to the control group. APN expression also increased, but for the lowest and the highest threonine levels (1.75 and 7%). At 21 d, there was no effect of threonine on the expression of MUC2, PepT1, and APN. In conclusion, in ovo threonine feeding beneficially affected the morphological and functional development of the intestinal mucosa, which ensured improved performance of chicks at hatch and at 21 d.
Assuntos
Galinhas/fisiologia , Intestino Delgado/efeitos dos fármacos , Treonina/farmacologia , Âmnio , Animais , Antígenos CD13/genética , Antígenos CD13/metabolismo , Embrião de Galinha , Galinhas/crescimento & desenvolvimento , Expressão Gênica , Íleo/metabolismo , Intestino Delgado/crescimento & desenvolvimento , Mucinas/genética , Mucinas/metabolismo , Transportador 1 de Peptídeos/genética , Transportador 1 de Peptídeos/metabolismo , Treonina/administração & dosagemRESUMO
O conhecimento da demanda e partição nutricional durante o ciclo pode contribuir significativamente para o manejo adequado da cultura. O presente trabalho objetivou determinar o acúmulo de massa, taxas de absorção e particionamento dos micronutrientes ferro (Fe), manganês (Mn), boro (B), zinco (Zn) e cobre (Cu), além do alumínio (Al), no tabaco (Nicotiana tabacum L.), utilizando uma variedade amplamente cultivada no sul do Brasil. O trabalho foi conduzido em propriedade particular produtora de tabaco localizada no município de Irati, PR, com delineamento experimental em blocos ao acaso com três repetições. Os tratamentos se constituíram de dez épocas de coleta de plantas, em intervalos de 15 dias. Foram avaliados a massa de matéria seca, conteúdo e taxas de acúmulo diárias dos micronutrientes e Al no caule e folhas da planta. Verificou-se que o máximo acúmulo de matéria seca foi próximo aos 68 dias, após o transplantio (DAT), e o pico de acúmulo para a maioria dos micronutrientes ocorreu a partir dos 105 DAT. A ordem de acúmulo de micronutrientes para o tabaco foi Fe>Mn>B>Zn>Cu com os valores máximos obtidos aos 105, 135, 135, 135 e 110 DAT, respectivamente, onde mais de 60% dos micronutrientes absorvidos foram depositados nas folhas. O acúmulo de Al foi de 36 kg ha-1, com 98,2% depositado nas folhas.
Despite the historical and consolidated cultivation of tobacco in Brazil, knowledge of the demand and nutritional partition during the cycle can contribute significantly to the proper management of the crop. The objective of this study was to determine mass accumulation, absorption rates and partitioning of iron (Fe), manganese (Mn), boron (B), zinc (Zn), copper (Cu) and aluminum (Al) in tobacco (Nicotiana tabacum L.), using a widely cultivated variety in southern Brazil. This study was conducted on a tobacco property located in the municipality of Irati, PR, with a randomized block design with three replicates. The treatments consisted of ten plant collection periods at intervals of 15 days. Dry matter mass, content and daily accumulation rates of micronutrients and Al in the stem and leaves of the plant were evaluated. It was verified that the maximum accumulation of dry matter mass was close to 68 days after transplanting (DAT), and the peak of accumulation for most micronutrients occurred from 105 DAT. The order of micronutrient accumulation for tobacco was Fe>Mn>B>Zn>Cu, with maximum values obtained at 105, 135,135, 135 and 110 DAT, respectively, where more than 60% of the absorbed micronutrients were deposited on the leaves. The accumulation of Al was 36 kg ha-1, with 98.2% deposited on sheets.
Assuntos
Absorção , Alumínio/análise , Micronutrientes/análise , Nicotiana/químicaRESUMO
O conhecimento da demanda e partição nutricional durante o ciclo pode contribuir significativamente para o manejo adequado da cultura. O presente trabalho objetivou determinar o acúmulo de massa, taxas de absorção e particionamento dos micronutrientes ferro (Fe), manganês (Mn), boro (B), zinco (Zn) e cobre (Cu), além do alumínio (Al), no tabaco (Nicotiana tabacum L.), utilizando uma variedade amplamente cultivada no sul do Brasil. O trabalho foi conduzido em propriedade particular produtora de tabaco localizada no município de Irati, PR, com delineamento experimental em blocos ao acaso com três repetições. Os tratamentos se constituíram de dez épocas de coleta de plantas, em intervalos de 15 dias. Foram avaliados a massa de matéria seca, conteúdo e taxas de acúmulo diárias dos micronutrientes e Al no caule e folhas da planta. Verificou-se que o máximo acúmulo de matéria seca foi próximo aos 68 dias, após o transplantio (DAT), e o pico de acúmulo para a maioria dos micronutrientes ocorreu a partir dos 105 DAT. A ordem de acúmulo de micronutrientes para o tabaco foi Fe>Mn>B>Zn>Cu com os valores máximos obtidos aos 105, 135, 135, 135 e 110 DAT, respectivamente, onde mais de 60% dos micronutrientes absorvidos foram depositados nas folhas. O acúmulo de Al foi de 36 kg ha-1, com 98,2% depositado nas folhas.(AU)
Despite the historical and consolidated cultivation of tobacco in Brazil, knowledge of the demand and nutritional partition during the cycle can contribute significantly to the proper management of the crop. The objective of this study was to determine mass accumulation, absorption rates and partitioning of iron (Fe), manganese (Mn), boron (B), zinc (Zn), copper (Cu) and aluminum (Al) in tobacco (Nicotiana tabacum L.), using a widely cultivated variety in southern Brazil. This study was conducted on a tobacco property located in the municipality of Irati, PR, with a randomized block design with three replicates. The treatments consisted of ten plant collection periods at intervals of 15 days. Dry matter mass, content and daily accumulation rates of micronutrients and Al in the stem and leaves of the plant were evaluated. It was verified that the maximum accumulation of dry matter mass was close to 68 days after transplanting (DAT), and the peak of accumulation for most micronutrients occurred from 105 DAT. The order of micronutrient accumulation for tobacco was Fe>Mn>B>Zn>Cu, with maximum values obtained at 105, 135,135, 135 and 110 DAT, respectively, where more than 60% of the absorbed micronutrients were deposited on the leaves. The accumulation of Al was 36 kg ha-1, with 98.2% deposited on sheets.(AU)
Assuntos
Nicotiana/química , Absorção , Micronutrientes/análise , Alumínio/análiseRESUMO
O intestino dos insetos representa uma interface pouco protegida dos agentes externos. A identificação dos mecanismos moleculares envolvidos nos processos fisiológico-digestivos permite encontrar alvos potenciais para o controle de insetos. As moléculas envolvidas na absorção de nutrientes, tamponamento e geração de fluxos de água no intestino médio do inseto-modelo Musca domestica (Diptera Cyclorrhapha) foram identificadas. Para isso, foi feita uma análise da expressão gênica ao longo do intestino médio, a identificação e anotação de proteínas por bioinformática, a confirmação da localização apical das proteínas por análise proteômica de membranas microvilares purificadas e a determinação do papel de algumas das proteínas através de experimentos in vivo utilizando diferentes dietas, corantes e inibidores. A acidificação da região média é consequência da atividade anidrase carbônica que gera prótons que são bombeados por uma H+ V-ATPase apical acompanhados por cloreto transportado por um simporter K+Cl-. O K+ é recuperado por um canal de K+ e a homeostase dos cátions mantida pela Na+/K+-ATPase basolateral. O bicarbonato é eliminado basolateralmente em troca por cloreto por um antiporter. A acidificação é regulada diretamente por um antiporter Na+/H+ e indiretamente por uma proteína envolvida na homeostase do cobre. O muco protetor da região média é tamponado com bicarbonato gerado por uma anidrase carbônica com âncora de GPI e transportado por um antiporter Na+HCO3-/H+Cl-. O excesso de ácido é eliminado por um antiporter Na+/H+ situado na membrana basolateral. A alcalinização da região posterior ocorre pelo transporte apical de NH3 que sequestra os prótons luminais gerando amônio, junto à remoção de prótons em simporte com aminoácidos e peptídeos. A acidificação intracelular, consequência da entrada de aminoácidos com prótons, é regulada por uma H+ V-ATPase basolateral. A geração de fluxos de água é consequência da atividade conjunta de simporters NKCC e KCC ajudados pelas aquaporinas. A inibição dos simporters com inibidores específicos mostrou que o contrafluxo de água está envolvido na reciclagem da enzima tripsina. Por último, o principal lugar de absorção nutrientes no intestino médio é a região posterior, a exceção do cobre que é absorvido na região média
The gut of insects is a less protected interphase against external agents. The identification of the molecular mechanisms involved in physiological-digestive processes allows one to find potential targets for insect control. The molecules involved in nutrient absorption, buffering and fluid fluxes in the midgut of the insect-model M. domestica (Diptera Cyclorrhapha) were identified. For this, gene expression along the midgut was analyzed; proteins were identified and annotated by bioinformatics; apical localization of proteins was confirmed by proteomics of purified microvillar membranes; the role of proteins was confirmed by in vivo experiments using different diets, dyes and inhibitors. Middle midgut acidification occurs by the action of an apical H+ V-ATPase with protons coming from carbonic anhydrase activity accompanied by chloride transported with potassium by a K+Cl- symporter. Potassium is recovered by a potassium channel, and cation homeostasis maintained by a basolateral Na+/K+-ATPase. Acidification is directly regulated by a Na+/H+ antiporter and indirectly by a copper homeostasis protein. Mucus protecting epithelium is neutralized with bicarbonate generated by a GPI-ancored carbonic anhydrase and transported by a Na+HCO3-/H+Cl- antiporter. Intracellular acidification is avoided by a basolateral Na+/H+ antiporter. Posterior midgut alkalization occurs by the action of an apical ammonia transporter and proton amino acid (and peptide) symporters. Intracellular acid is eliminated by a basolateral H+ V-ATPase. Fluid fluxes are generated by K+Cl- and Na+Cl-Cl- symporters helped by aquaporins. Inhibition of these symporters showed that the countercurrent flux of water allows trypsin recycling. Finally, posterior midgut is the main location of nutrient absorption, except for copper which is absorbed in the middle midgut
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Nutrientes/efeitos adversos , Bases de Dados de Compostos Químicos , Moscas Domésticas/patogenicidade , Insetos , IntestinosRESUMO
Physiological data showed that T. molitor midgut is buffered at pH 5.6 at the two anterior thirds and at 7.9 at the posterior third. Furthermore, water is absorbed and secreted at the anterior and posterior midgut, respectively, driving a midgut counter flux of fluid. To look for the molecular mechanisms underlying these phenomena and nutrient absorption as well, a transcriptomic approach was used. For this, 11 types of transporters were chosen from the midgut transcriptome obtained by pyrosequencing (Roche 454). After annotation with the aid of databanks and manual curation, the sequences were validated by RT-PCR. The expression level of each gene at anterior, middle and posterior midgut and carcass (larva less midgut) was evaluated by RNA-seq taking into account reference sequences based on 454 contigs and reads obtained by Illumina sequencing. The data showed that sugar and amino acid uniporters and symporters are expressed along the whole midgut. In the anterior midgut are found transporters for NH3 and NH4+ that with a chloride channel may be responsible for acidifying the lumen. At the posterior midgut, bicarbonate-Cl- antiporter with bicarbonate supplied by carbonic anhydrase may alkalinize the lumen. Water absorption caused mainly by an anterior Na+-K+-2Cl- symporter and water secretion caused by a posterior K+-Cl- may drive the midgut counter flux. Transporters that complement the action of those described were also found.
Assuntos
Tenebrio/metabolismo , Aminoácidos/metabolismo , Fenômenos Fisiológicos da Nutrição Animal , Animais , Transporte Biológico , Trato Gastrointestinal/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Larva/metabolismo , Monossacarídeos/metabolismo , Tenebrio/crescimento & desenvolvimento , Água/metabolismoRESUMO
En el tratamiento nutricional de la diarrea infantil se han utilizado con éxito preparaciones líquidas que contienen pollo combinado con cereales y/o tubérculos, que se preparan en la institución de hospitalización o en la casa. En un estudio anterior, se comparó la efectividad de fórmulas con proteínas de pollo o aislado de soja en el tratamiento de la diarrea infantil. El objetivo de este trabajo fue estudiar en ratas con o sin diarrea, la utilización de las proteínas y la disponibilidad de nutrientes de dietas preparadas con pollo o aislado proteico de soja, para determinar si los resultados obtenidos en los niños eran comparables a los obtenidos en ratas. Dado que la diarrea y la desnutrición frecuentemente están presentes de manera simultánea, también se estudiaron grupos de animales con o sin diarrea previamente desnutridos. Los resultados mostraron que durante la diarrea las ratas desnutridas fueron igualmente eficientes que las nutridas en utilizar los nutrientes presentes en las dietas con pollo o soja, por lo que no experimentaron un deterioro nutricional mayor que las nutridas. Adicionalmente, la severidad de la diarrea fue similar en las ratas asignadas a las dietas con pollo o soja. Asimismo, las ratas con diarrea consumieron, crecieron, absorbieron y retuvieron menos de los macronutrientes dietarios que las ratas control. Sin embargo, este efecto de la diarrea fue similar en las ratas que consumieron proteína de soja o de pollo. Como estos resultados coinciden con los obtenidos previamente en niños con diarrea aguda, se concluye que la proteína de soja es tan efectiva como la de pollo en el manejo nutricional de la diarrea y que la diarrea producida con lactosa en ratas es un modelo apropiado para el estudio de las consecuencias nutricionales de la misma.
In the nutritional treatment of diarrhea, good results have been obtained using liquid formulas with chicken meat together with cereals and/or starchy vegetables, prepared at home or at the clinic. In a previous study, formulas prepared with chicken meat or isolated soybean protein were tested in the treatment of infantile diarrhea. In order to determine if the results obtained in children could be reproduced in rats, the objective of the present study was to evaluate the effectiveness of the same diets in the nutritional treatment of well-nourished as well as undernourished rats with lactose induced diarrhea. The results showed that all the rats with diarrhea absorbed and retained less of the dietary nutrients and consequently, grew less than their counterparts without diarrhea. However, the absorption and retention measured in the nourished as well as in the undernourished rats were similar, indicating that a short period of malnutrition previous to the diarrhea episode, did not affect rats' absorption capacity. In addition, similarly to the results previously obtained in children, the rats with lactose induced diarrhea utilised equally well the dietary nutrients present in the chicken as well as in the soybean protein based diets. These results confirmed the value of isolated soybean protein in the nutritional treatment of diarrhea and showed that lactose induced diarrhea in rats is an appropriate model to study the nutritional consequences of diarrhea.
Assuntos
Ratos , Diarreia Infantil , Diarreia Infantil/dietoterapia , Alimentos , Lactose/efeitos adversos , Desnutrição , Proteínas/administração & dosagem , Glycine max , Galinhas/genética , RatosRESUMO
O fornecimento de nutrientes em quantidades adequadas e em proporções equilibradas é fundamental no processo produtivo das pastagens. Para o manejo da adubação, torna-se importante conhecer a necessidade de nutrientes das plantas forrageiras e, consequentemente, sua capacidade de extraí-los do solo. Diante disso, objetivou-se avaliar a extração de nutrientes pela fitomassa de cultivares de Brachiaria brizantha sob doses de nitrogênio. O delineamento experimental utilizado foi blocos completos ao acaso, com três repetições, em esquema de parcelas repetidas no tempo. Nas parcelas empregou-se o esquema fatorial 3 x 4, sendo três cultivares de Brachiaria brizantha (MG-4, Marandu e Xaraés) e quatro doses de nitrogênio (0, 50, 100 e 150 mg.dm-3). No tempo foram alocados os três cortes de avaliação, referente às épocas dos cortes. Como fonte de nitrogênio utilizou-se a ureia. O capim-xaraés mostrou maior produtividade, quando comparado aos outros cultivares, sendo considerado mais responsivo à adubação nitrogenada. O efeito mais pronunciado sobre a produção de fitomassa dos cultivares de Brachiaria brizantha e extração dos nutrientes foi proporcionado pelas maiores doses de nitrogênio. A máxima extração de macronutrientes pelos cultivares de Brachiaria brizantha seguiu a ordem decrescente para os macronutrientes K > N > P > Mg.> S e micronutrientes Mn > Fe > Zn > Cu, sendo necessário repor os nutrientes do solo, com o aumento da adubação nitrogenada.
Adequate amounts and balanced proportions of nutrient supply is fundamental for the forage productive process. For the fertilization handling, forage plant nutrient requiriment must be known and, consequently, the plant capacity to extract soil nutrients. Therefore, the research had as objective to evaluate the nutrient extraction by Brachiaria brizantha phytomass under nitrogen doses. A randomized complete block experimental design with three replications in a plot repeated in time was used. In the plots, a factorial 3 x 4 arrangement was used, being three cultivars of Brachiaria brizantha (MG-4, Marandu and Xaraés) and four nitrogen doses (0, 50, 100 and 150 mg.dm-3). Three cuts of evaluation were allocated in time, referring. to the time of cuts. The source of nitrogen used was urea. The sharpest treatment effects on phytomass production of Brachiaria brizantha cultivars and nutrient extraction were influenced by the highest nitrogen doses. Xaraés-grass showed greater productivity in relation to the other cultivars, being.considered more responsive to the nitrogen fertilization. The maximum macronutrient extraction by Brachiaria brizantha followed the decreasing.order for the macronutrients: K > N > P > Mg.> S and micronutrients: Mn > Faith > Zn > Cu, being. necessary to restore soil nutrients, with the increase of nitrogen.
Assuntos
Brachiaria , Fertilizantes , Necessidades Nutricionais , Agricultura , Compostos de NitrogênioRESUMO
O fornecimento de nutrientes em quantidades adequadas e em proporções equilibradas é fundamental no processo produtivo das pastagens. Para o manejo da adubação, torna-se importante conhecer a necessidade de nutrientes das plantas forrageiras e, consequentemente, sua capacidade de extraí-los do solo. Diante disso, objetivou-se avaliar a extração de nutrientes pela fitomassa de cultivares de Brachiaria brizantha sob doses de nitrogênio. O delineamento experimental utilizado foi blocos completos ao acaso, com três repetições, em esquema de parcelas repetidas no tempo. Nas parcelas empregou-se o esquema fatorial 3 x 4, sendo três cultivares de Brachiaria brizantha (MG-4, Marandu e Xaraés) e quatro doses de nitrogênio (0, 50, 100 e 150 mg.dm-3). No tempo foram alocados os três cortes de avaliação, referente às épocas dos cortes. Como fonte de nitrogênio utilizou-se a ureia. O capim-xaraés mostrou maior produtividade, quando comparado aos outros cultivares, sendo considerado mais responsivo à adubação nitrogenada. O efeito mais pronunciado sobre a produção de fitomassa dos cultivares de Brachiaria brizantha e extração dos nutrientes foi proporcionado pelas maiores doses de nitrogênio. A máxima extração de macronutrientes pelos cultivares de Brachiaria brizantha seguiu a ordem decrescente para os macronutrientes K > N > P > Mg.> S e micronutrientes Mn > Fe > Zn > Cu, sendo necessário repor os nutrientes do solo, com o aumento da adubação nitrogenada.(AU)
Adequate amounts and balanced proportions of nutrient supply is fundamental for the forage productive process. For the fertilization handling, forage plant nutrient requiriment must be known and, consequently, the plant capacity to extract soil nutrients. Therefore, the research had as objective to evaluate the nutrient extraction by Brachiaria brizantha phytomass under nitrogen doses. A randomized complete block experimental design with three replications in a plot repeated in time was used. In the plots, a factorial 3 x 4 arrangement was used, being three cultivars of Brachiaria brizantha (MG-4, Marandu and Xaraés) and four nitrogen doses (0, 50, 100 and 150 mg.dm-3). Three cuts of evaluation were allocated in time, referring. to the time of cuts. The source of nitrogen used was urea. The sharpest treatment effects on phytomass production of Brachiaria brizantha cultivars and nutrient extraction were influenced by the highest nitrogen doses. Xaraés-grass showed greater productivity in relation to the other cultivars, being.considered more responsive to the nitrogen fertilization. The maximum macronutrient extraction by Brachiaria brizantha followed the decreasing.order for the macronutrients: K > N > P > Mg.> S and micronutrients: Mn > Faith > Zn > Cu, being. necessary to restore soil nutrients, with the increase of nitrogen.(AU)
Assuntos
Brachiaria , Fertilizantes , Necessidades Nutricionais , Agricultura , Compostos de NitrogênioRESUMO
Iron-defi ciency anemia affects many groups, with a prevalence of more than 50% among preschoolers in Brazil. One of the many possible approaches to defeat the problem is the regular and suffi cient offer of dietary iron, combined with absorption agonists in the same meal. The objective of the present work was testing the quality of the diet offered to preschoolers assisted by the National School Meal Program (PNAE û Programa Nacional de Alimentação Escolar) in Campinas, in the countryside of São Paulo state. Energy (Kcal), total iron (mg) and absorbable iron (mg) were measured. The sample was composed by 167 children aged 25 to 72 months in 2003. After quality evaluation, only the data from 154 subjects were analyzed. Results showed that iron consumption is higher among girls: 73% of the girls presented a consumption above the RDA value, against 51% of the boys (p<0.05). There were no statistical differences for energy (p = 0.069), total iron (p = 0.395) and absorbable iron (p = 0.353) consumption between the genders. Absorbable iron in preschoolers' meals is expected to be as much as an average 5% of the total iron intake. However, the main reason for this low absorption is not the poor frequency or the scarcity of dietary iron sources, but the limited offer of agonistic absorption factors (mainly meat and vitamin C) in the meals.
Pre-escolares brasileños son un grupo de riesgo para anemia ferropriva, con prevalencia superior a 50%. Entre las estrategias para combatir el problema está la oferta adecuada del mineral y la presencia concomitante de mejoradores de su absorción. El objetivo del estudio fue evaluar la calidad de la dieta de pre-escolares atendidos por el Programa Nacional de Alimentación Escolar (PNAE) en el municipio de Campinas, estado de São Paulo. Fueron analizadas dietas ofrecidas durante 2003, cuanto al aporte de energía (Kcal) e hierro (mg) así como el hierro absorbido. De un grupo de 167 infantes de 25 a 72 meses de edad, 154 valoraciones fueron consideradas válidas. Los resultados evidenciaron que el consumo de hierro es mejor en niñas, 73% mostraron ingestión adecuada, contra 51% en niños (p<0,05). Los valores encontrados para energía (p=0,069), hierro total (p=0,395) y hierro absorbido (p=0,353) fueron semejantes para ambos sexos. La absorción del hierro de la dieta se mostró baja (5 a 6% del total) y la razón principal no era su ausencia, sino la limitada presencia de mejoradores de la absorción como carne y vitamina C.
O grupo de pré-escolares brasileiros está entre os considerados de risco para a anemia ferropriva, com uma prevalência superior a 50%. Entre asestratégias para combater o problema, está a oferta adequada e sufi ciente do mineral, e a presença de agonistas de absorção às mesmasrefeições. O objetivo deste trabalho foi avaliar a qualidade da dieta de pré-escolares atendidos pelo Programa Nacional de Alimentação Escolar(PNAE) no município de Campinas, SP quanto ao fornecimento de energia e ferro, por meio da quantifi cação da energia (Kcal), do ferro total (mg), e do ferro absorvido (mg) a partir das refeições consumidas no ano de 2003. A amostra foi composta por 167 crianças de 25 a 72 meses;154 inquéritos foram considerados válidos. Os resultados mostraram que o consumo de ferro é melhor entre as meninas, uma vez que 73%apresentaram valores de ingestão adequados, contra 51% dos meninos (p < 0,05). Os valores encontrados para energia (p = 0,069), ferro total(p = 0,395) e ferro absorvido (p = 0,353) não diferiram entre os gêneros. A dieta consumida pelas crianças deve resultar em baixa absorçãodo mineral (estimativa de 5 a 6% do total); a principal razão para isto, contudo, não está na pequena freqüência ou quantidade de alimentosfonte de ferro, mas sim na limitada oferta de agentes agonistas da absorção, como carne e vitamina C.
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Anemia Ferropriva , Ferro/deficiência , Nutrientes , Alimentação Escolar , Absorção , Programas de NutriçãoRESUMO
O experimento foi conduzido em casa de vegetação, na Embrapa Arroz e Feijão, com o objetivo de avaliar o intervalo de corte na produção de massa seca, altura de planta e composição químico-bromatológica da Brachiaria brizantha cv. MG-5. O delineamento utilizado foi o inteiramente casualizado. Os tratamentos constituíram de quatro intervalos de corte (15, 20, 30 e 60 dias), com cinco repetições, totalizando 20 parcelas. Foram aplicados 100 mg dm-3 de nitrogênio, utilizando como fonte o sulfato de amônio, em todas as parcelas. A adubação nitrogenada foi parcelada, de acordo com os cortes de avaliações. Foram realizados quatro cortes para o intervalo de 15 dias, três cortes para o de 20 dias, dois cortes para o de 30 dias e um corte para o de 60 dias. A forrageira foi cortada a uma altura de 5 cm do solo. O aumento no intervalo de corte na Brachiaria brizantha cv. M-5 determina acréscimos na produção de massa seca e teores de FDN e FDA. Contudo, o inverso pode ser observado no teor de PB e concentrações de P, Cu e Fe.
The experiment was carried out in a green house, at Embrapa Arroz e Feijão. It aimed to evaluate the offect of cutting period on dry mass production and bromatologic- chemical composition of Brachiaria brizantha cv. MG-5. The experimental design was completely randomized, with five replications. The treatments were constituted by four cutting intervals (15, 20, 30 and 60 days). After cuttings at a distant from soil, of ammonium sulfate (100 mg dm-3) was applied to all treatments. Four cuttings were carried out at 15 - day intervals, three at 20 day-intervals, two at 30 - day intervals and one at a 60 day-interval. Increases in cutting intervals caused dry mass production, neutral detergent fiber and acid detergent fiber rase. However, the opposite was observed for bromatologic composition and phosphorus, copper and iron concentrations.
RESUMO
Anato plant seeds (Bixa orellana var. Peruana) were germinated in a soil + organic matter (2:1) substrate that received triple superphosphate (1.5 g/l). After 115 days, plants were transplanted to a high fertility Dark Red Latosol. Spacing was 5m x 5m a top dressing with 5g of KCl and l0g urea per plant was applied at the 201 day. Starting on day 115 plants (4 per sampling) were collected at 60 day intervals until the 507 day. Samples were subdivided in leaves, branches, stem and reproductive parts. At the 507 day fruits were taken and divided into seeds and hulls. Anato plants grew slowly until the 267 day and improved growth rate from then on to the 447 day, when growth almost stopped. The macro and micronutrient extraction by a 400 plant per hectare crop at the 507 day were: N - 25.7 kg, P - 4.1 kg, K - 16.7 kg, Ca - 16.4 kg, Mg - 4.4 kg, S - 2.5 kg, B - 56.4 g, Cu - 16.2 g, Fe - 240.1 g, Mn - 130.3 g and Zn - 50.7 g.
Sementes de urucu (Bixa orellana L.) var. Peruana foram postas para germinar num substrato constituido de duas partes de terra para uma de matéria orgânica, onde foram adicionados 1,5g de superfosfato triplo por litro de substrato. Após 115 dias as plantas foram transplantadas para um Latossolo Vermelho Escuro Orto série "Luiz de Queiroz" de alta fertilidade natural. O espaçamento foi de 5m x 5m. Aos 201 dias foi feita uma adubação em cobertura com 5g de KCl + 10g de uréia por planta. Plantas foram coletadas a partir dos 115 dias com intervalos de 60 dias até a produção aos 507 dias. As plantas em número de quatro por amostragem foram subdivididas em folhas, ramos, caule e órgãos reprodutivos. Aos 507 dias foram coletados frutos que foram subdivididos em casca e sementes. Os resultados mostraram que o urucu cresce lentamente até aos 267 dias intensificando o seu crescimento até os 447 dias quando ocorre uma paralização. A extração de macro e micronutrientes por uma cultura de 400 plantas/ha aos 507 dias foi de: N - 25,7kg, P - 4,1kg, K -16,7kg, Ca - 16,4kg, Mg - 4,4kg, S - 2,5kg, B - 56,4g, Cu - 16,2g, Fe - 240- 130,3g e Zn - 50,7g.