RESUMO
This study aimed to investigate the isolated effects of NaHCO3 on cycling time-trial performance. Furthermore, we investigated whether the ingestion time of NaHCO3, standardized or individualized based on time to peak, could be effective in improving cycling time-trial performance. A systematic review was carried out on randomized placebo-controlled studies. A random-effects meta-analysis assessed the standardized mean difference (SMD) between NaHCO3 and placebo conditions. Eighteen studies were qualitatively (systematic review) and quantitatively (meta-analysis) analysed concerning mean power output (Wmean) (n = 182) and time performance (n = 201). The reviewed studies showed a low risk of bias and homogenous results for Wmean (I2 = 0%) and performance time (I2 = 0%). Overall, when compared to placebo, the NaHCO3 ingestion improved the Wmean (SMD: 0.42; 95% CI: 0.21-0.63; P = 0.001) and performance time (SMD: 0.22; 95% CI: 0.02-0.43; P = 0.03). Similarly, the NaHCO3 ingestion using a time-to-peak strategy improved the Wmean (SMD: 0.39; 95% CI: 0.03-0.75; P = 0.04; I2 = 15%) and performance time (SMD: 0.34; 95% CI: 0.07-0.61, P = 0.01, I2 = 0%). The present findings reveal that NaHCO3 ingestion has the potential to increase the overall performance time and Wmean in cycling time trials.HighlightsNaHCO3 is an effective strategy to increase cycling time-trial performance.The standardized protocol did not improve the cycling time-trial performance parameters.The individualized time-to-peak NaHCO3 ingestion has a positive effect on time and Wmean during cycling time-trial performance.
Assuntos
Ciclismo , Bicarbonato de Sódio , Humanos , Bicarbonato de Sódio/farmacologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Ingestão de AlimentosRESUMO
High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is unclear. We examined seven high-altitude waterfowl that have inhabited the Andes (3812-4806 m elevation) over varying evolutionary time scales, to elucidate changes in biochemical pathways of energy metabolism in flight muscle relative to low-altitude sister taxa. Convergent changes across high-altitude taxa included increased hydroxyacyl-coA dehydrogenase and succinate dehydrogenase activities, decreased lactate dehydrogenase, pyruvate kinase, creatine kinase, and cytochrome c oxidase activities, and increased myoglobin content. ATP synthase activity increased in only the longest established high-altitude taxa, whereas hexokinase activity increased in only newly established taxa. Therefore, changes in pathways of lipid oxidation, glycolysis, and mitochondrial oxidative phosphorylation are common strategies to cope with high-altitude hypoxia, but some changes require longer evolutionary time to arise.
Assuntos
Anseriformes/metabolismo , Evolução Biológica , Metabolismo Energético , Músculo Esquelético/metabolismo , Altitude , Distribuição Animal , Animais , América do SulRESUMO
Torrent ducks inhabit fast-flowing rivers in the Andes from sea level to altitudes up to 4500â m. We examined the mitochondrial physiology that facilitates performance over this altitudinal cline by comparing the respiratory capacities of permeabilized fibers, the activities of 16 key metabolic enzymes and the myoglobin content in muscles between high- and low-altitude populations of this species. Mitochondrial respiratory capacities (assessed using substrates of mitochondrial complexes I, II and/or IV) were higher in highland ducks in the gastrocnemius muscle - the primary muscle used to support swimming and diving - but were similar between populations in the pectoralis muscle and the left ventricle. The heightened respiratory capacity in the gastrocnemius of highland ducks was associated with elevated activities of cytochrome oxidase, phosphofructokinase, pyruvate kinase and malate dehydrogenase (MDH). Although respiratory capacities were similar between populations in the other muscles, highland ducks had elevated activities of ATP synthase, lactate dehydrogenase, MDH, hydroxyacyl CoA dehydrogenase and creatine kinase in the left ventricle, and elevated MDH activity and myoglobin content in the pectoralis. Thus, although there was a significant increase in the oxidative capacity of the gastrocnemius in highland ducks, which correlates with improved performance at high altitudes, the variation in metabolic enzyme activities in other muscles not correlated to respiratory capacity, such as the consistent upregulation of MDH activity, may serve other functions that contribute to success at high altitudes.