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Maca (Lepidium meyenii), a biennial herbaceous plant indigenous to the Andes Mountains, has a rich history of traditional use for its purported health benefits. Maca's chemical composition varies due to ecotypes, growth conditions, and post-harvest processing, contributing to its intricate phytochemical profile, including, macamides, macaenes, and glucosinolates, among other components. This review provides an in-depth revision and analysis of Maca's diverse bioactive metabolites, focusing on the pharmacological properties registered in pre-clinical and clinical studies. Maca is generally safe, with rare adverse effects, supported by preclinical studies revealing low toxicity and good human tolerance. Preclinical investigations highlight the benefits attributed to Maca compounds, including neuroprotection, anti-inflammatory properties, immunoregulation, and antioxidant effects. Maca has also shown potential for enhancing fertility, combating fatigue, and exhibiting potential antitumor properties. Maca's versatility extends to metabolic regulation, gastrointestinal health, cardio protection, antihypertensive activity, photoprotection, muscle growth, hepatoprotection, proangiogenic effects, antithrombotic properties, and antiallergic activity. Clinical studies, primarily focused on sexual health, indicate improved sexual desire, erectile function, and subjective wellbeing in men. Maca also shows promise in alleviating menopausal symptoms in women and enhancing physical performance. Further research is essential to uncover the mechanisms and clinical applications of Maca's unique bioactive metabolites, solidifying its place as a subject of growing scientific interest.
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Maca (Lepidium meyenii, Lepidium peruvianum) is part of the Brassicaceae family and grows at high altitudes in the Peruvian Andes mountain range (3500-5000 m). Historically, it has been used as a nutrient-dense food and for its medicinal properties, primarily in enhancing energy and fertility. Scientific research has validated these traditional uses and other clinical applications by elucidating maca's mechanisms of action, nutrition, and phytochemical content. However, research over the last twenty years has identified up to seventeen different colors (phenotypes) of maca. The color, hypocotyl size, growing location, cultivation, and post-harvest processing methods can have a significant effect on the nutrition content, phytochemical profile, and clinical application. Yet, research differentiating the colors of maca and clinical applications remains limited. In this review, research on the nutrition, phytochemicals, and various colors of maca, including black, red, yellow (predominant colors), purple, gray (lesser-known colors), and any combination of colors, including proprietary formulations, will be discussed based on available preclinical and clinical trials. The gaps, deficiencies, and conflicts in the studies will be detailed, along with quality, safety, and efficacy criteria, highlighting the need for future research to specify all these factors of the maca used in publications.
Assuntos
Lepidium , Extratos Vegetais , Extratos Vegetais/farmacologia , Fertilidade , Estado Nutricional , PeruRESUMO
The study considered the use of soil herbicides: Begin Turbo, KS; Dual Gold, KE; Euro-Lighting, VRK; Command, KE; Pivot, VK; Proponite, KE; Zenkor Ultra, KS and partially soil action: Demetra, KE, and Dialen Super, KS. We conducted a comparative assessment of the biological effectiveness of the studied herbicides against the main species of weeds present in the experimental plots, annual and perennial dicotyledonous, annual cereal weeds. The effect of soil herbicide treatments on the physiological state of plants of apple, pear, walnut, and black currant was studied. The effect of the use of the studied drugs on the yield of protected crops for three years was evaluated. The tests proved the applicability of soil herbicides in nursery, production gardens, as well as on seedlings with a closed root system. The tested products, despite the principle of their action - penetration into weeds through the soil, did not harm the protected crops, no negative effect on the growth of trees and shrubs was recorded. The study revealed no evidence that drugs had a negative impact on fruit and berry crop productivity. There are suggestions for improving the efficacy of using soil herbicides when planting fruit and berry crops.
O estudo considerou o uso dos herbicidas de solo: Begin Turbo, KS; Dual Gold, KE; Euro-Lighting, VRK; Command, KE; Pivot, VK; Proponite, KE; Zenkor Ultra, KS e ação parcial do solo: Demetra, KE e Dialen Super, KS. Realizou-se uma avaliação comparativa da eficácia biológica dos herbicidas estudados contra as principais espécies de plantas daninhas presentes nas parcelas experimentais, dicotiledôneas anuais e perenes, plantas daninhas de cereais anuais. Estudou-se o efeito de tratamentos com herbicidas de solo sobre o estado fisiológico de plantas de macieira, pera, nogueira e groselha preta. Foi avaliado o efeito do uso das drogas estudadas na produtividade de cultivos protegidos por três anos. Os testes comprovaram a aplicabilidade de herbicidas de solo em viveiros, hortas de produção, bem como em mudas com sistema radicular fechado. Os produtos testados, apesar de seus princípios de ação - penetração em ervas daninhas através do solo, não prejudicou as culturas protegidas, não foi registrado nenhum efeito negativo no crescimento de árvores e arbustos. O estudo não revelou evidências de que as drogas tenham um impacto negativo na produtividade das frutas e bagas. Existem sugestões para melhorar a eficácia do uso de herbicidas de solo ao plantar frutas e bagas.
Assuntos
Jardinagem , Plantas Daninhas/efeitos dos fármacos , Frutas/efeitos dos fármacos , Herbicidas/administração & dosagemRESUMO
Abstract Bacteria were isolated from samples of Fresh Apple juices from shops of three different localities of Lahore. Analysis of samples from Liberty, Anarkali and Yateem khana Markets show different levels of contamination. There were pathogenic and non-pathogenic bacteria in all samples and were identified by the morphological and biochemical tests. Most of the plasmids of pathogenic bacteria were 4kb in their molecular size. Ribotyping of 16S ribosomal RNA gene sequencing was done to confirm Helicobacter pylori strain and Gluconobacter oxydans. The highest sensitivity of 210mm was shown by Enterobacter sp. against Aztheromysine disk (15µg) while Micrococcus sp. was highly resistant against all of the Antibiotics applied. The antibiotic resistance of pathogenic bacteria was also checked against Ricinus communis plant's extracts, all isolated bacterial pathogens were resistant but only, E.coli was inhibited at 300µl of the extracts. Presence of pathogenic bacteria in Apple juice samples was due to contamination of sewage water in drinking water while some of these pathogenic bacteria came from Apple's tree and other from store houses of fruits.
Resumo As bactérias foram isoladas de amostras de suco de maçã fresco de lojas de três diferentes localidades de Lahore. A análise de amostras dos mercados Liberty, Anarkali e Yateem khana mostram diferentes níveis de contaminação. Havia bactérias patogênicas e não patogênicas em todas as amostras e foram identificadas pelos testes morfológicos e bioquímicos. A maioria dos plasmídeos de bactérias patogênicas tinha 4 kb em seu tamanho molecular. A ribotipagem do sequenciamento do gene do RNA ribossômico 16S foi realizada para confirmar a cepa de Helicobacter pylori e Gluconobacter oxydans. A maior sensibilidade de 210 mm foi mostrada por Enterobacter sp. contra disco de azteromisina (15µg) enquanto Micrococcus sp. foi altamente resistente a todos os antibióticos aplicados. A resistência a antibióticos de bactérias patogênicas também foi verificada contra extratos de plantas de Ricinus communis, todos os patógenos bacterianos isolados foram resistentes, mas apenas E. coli foi inibida em 300µl dos extratos. A presença de bactérias patogênicas nas amostras de suco de maçã deveu-se à contaminação da água de esgoto na água potável, enquanto algumas dessas bactérias patogênicas vieram da árvore da maçã e outras de armazéns de frutas.
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Background and Aim: Lepidium meyenii Walp (Maca) is an herbaceous plant that grows in the Peruvian Andes and it has been widely used as a nutritional supplement and fertility enhancer and has been used in the treatment of a variety of diseases, such as rheumatism, respiratory disorders, and anemia. The most notable feature of Maca is its potent antioxidant capacity, which helps in the scavenging of free radicals and protection of cells from oxidative stress. This study aimed to evaluate the in vitro effect of Maca extract on thawed sperm cells from bulls. Materials and Methods: Three dilutions of 1, 10, and 100 mg/mL of Maca extract were incubated with frozen-thawed bovine semen and analyzed at 1, 3, and 24 h of exposure time, evaluating the activity of the extract on the DNA, motility, morphology, viability, integrity of the membrane and acrosome of spermatozoa. Results: The Maca extract improved the studied sperm parameters of motility, acrosome integrity, vitality, and DNA integrity of sperm cells at a concentration of 10 mg/mL, and at 1 mg/mL, an improvement was observed in the morphology and integrity of the membrane. However, the best activity of the Maca extract was observed on the DNA integrity of the sperm, which was effective at the three concentrations evaluated after 24 h of incubation. Conclusion: The results indicate that L. meyenii can help in maintaining spermatozoa cellular integrity after the frozen-thaw process, especially in the protection against DNA fragmentation. Therefore, Maca would be a feasible supplementation to protect sperm to maintain their fertile ability after thawing.
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The purpose of this study was to analyses the influence of seasonal variation on the chemical composition and antimicrobial, antioxidant and cytotoxicity activities of the essential oil (EO) extracted from the leaves of Eugenia pohliana. Chemical characterization of the samples - by gas chromatography-mass spectrometry - found 35 and 38 components for summer and winter, respectively, of the EO from E.â pohliana leaves, totaling 47 different compounds. Analysis of antioxidant capacity (DPPH, ABTS and TAC) revealed that the summer EO showed greater free radical scavenging capacity than the winter. Similarly, the summer EO exhibited superior antimicrobial potential (MIC=128-512 µg/mL and MMC=128-1024â µg/mL, compared to the winter EO (128-2048 µg/mL and 256-2048â µg/mL, respectively). Results showed that both oils had a low potential to cause hemolysis. This study provides new scientific evidence on the influence of seasonality on the pharmacological properties of E.â pohliana leaves and its potential for the development of herbal medicines.
Assuntos
Anti-Infecciosos , Eugenia , Óleos Voláteis , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Antioxidantes/química , Radicais Livres , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Folhas de Planta/química , Estações do AnoRESUMO
Apples are rich sources of ursolic acid (UA) and oleanolic acid (OA) which are the major and most prominent triterpenes in the peel of an apple. Pentacyclic triterpenes are ideal nutraceuticals due to their ability to reduce the risk of many life-threatening diseases such as cancer, cardiovascular and diabetes. This study was to determine the content of UA and OA in the apple peel extract from different cultivars grown in South Africa as well as the correlation of their content level with antioxidant capacity. Quantitative analysis of UA and OA in apple peels from three cultivars; red delicious (RD), royal gala (RG) and granny smith (GS) apples was carried out using HPLC and their antioxidant capacity was analyzed using the DPPH assay. The RD showed the highest content of UA and OA (248.02 ± 0.08 µg/ml and 110.00 ± 0.08 µg/ml respectively) in the apple peel extract and also displayed a significantly high level of antioxidant capacity (97.3 ± 0.40%; p < 0.0001) compared to the RG and GS cultivars. A strong positive correlation was noted between the UA, OA and antioxidant capacities of all the cultivars. Only the RD cultivar showed a significant correlation though; UA (r = 0.9570; p = 0.0027) and OA (r = 0.8503; p = 0.0319). This study demonstrated that the RD and RG apple peels possess the highest UA and OA content which invariably increases their antioxidant activities compared to GS apple. Thus, both apple cultivars would be useful and recommended for food consumption and nutraceuticals values to improve human health.
As maçãs são fontes ricas em ácido ursólico (UA) e ácido oleanólico (OA), que são os principais e mais proeminentes triterpenos na casca de uma maçã. Os triterpenos pentacíclicos são nutracêuticos ideais devido à sua capacidade de reduzir o risco de muitas doenças potencialmente fatais, como câncer, doenças cardiovasculares e diabetes. Este estudo teve como objetivo determinar o conteúdo de UA e OA no extrato de casca de maçã de diferentes cultivares cultivadas na África do Sul, bem como a correlação do seu nível de conteúdo com a capacidade antioxidante. Análise quantitativa de UA e OA em cascas de maçã de três cultivares maçãs red delicious (RD), royal gala (RG) e granny smith (GS) foi realizada por meio de HPLC e sua capacidade antioxidante foi analisada pelo ensaio DPPH. A RD apresentou o maior teor de UA e OA (248,02 ± 0,08 µg/ml e 110,00 ± 0,08 µg/ml respectivamente) no extrato de casca de maçã e também apresentou um nível significativamente alto de capacidade antioxidante (97,3 ± 0,40%; p <0,0001) em comparação com as cultivares RG e GS. Uma forte correlação positiva foi observada entre o UA, OA e as capacidades antioxidantes de todas as cultivares. Porém, apenas a cultivar RD apresentou correlação significativa; UA (r = 0,9570; p = 0,0027) e OA (r = 0,8503; p = 0,0319). Este estudo demonstrou que as cascas de maçã RD e RG possuem o maior teor de UA e OA, o que invariavelmente aumenta suas atividades antioxidantes em comparação com a maçã GS. Assim, ambas as cultivares de maçã seriam úteis e recomendadas para consumo alimentar e valores nutracêuticos para melhorar a saúde humana.
Assuntos
Ácido Oleanólico , Triterpenos , Frutas , AntioxidantesRESUMO
The wax apple or jambu madu, is a non-climacteric tropical fruit from Myrtaceae family and widely cultivated in South East Asia. The limited availability of good quality seedlings of wax apple is the main problem to development of flourish it's market share in the current fruit industry. Therefore, in order to produce good quality planting materials, a study aimed at optimizing propagation and adventitious rooting technique and survivability of wax apple air layer was conducted. In this study, four different levels of Indole-3-Butyric Acid (IBA) concentration (0, 1000, 1500 and 2000 mg L-1) and three rooting media (sphagnum moss, vermicompost and garden soil) were applied after removal of bark (phloem) on the shoot to determine the effect on rooting and survivability of the wax apple air layer under field conditions. The results showed that the wax apple shoots treated with 2000 mg L-1 IBA produced the significantly higher number of roots, increased length of root, diameter of branch, length of branch, number of leaf and leaf area of air layers. In addition, the highest chlorophyll content and stomatal aperture were recorded in 2000 mg L-1 IBA treatment compared to other treatments including control. Vermicompost medium was better than garden soil and sphagnum moss in respect of rooting and survivability of air layers. The results showed that the combination of 2000 mg L-1 IBA and vermicompost as rooting media give the best combination to root initiation, root number, root length and survival rate (100%) of wax apple air layers. From this study, it can be concluded that 2000 mg L-1 IBA and vermicompost treatment enhance the root initiation, early establishment and survivability of wax apple air layered under field conditions.
A maçã de cera, ou jambu madu, é uma fruta tropical não climatérica da família Myrtaceae e amplamente cultivada no Sudeste Asiático. A disponibilidade limitada de mudas de macieira de boa qualidade é o principal problema para o desenvolvimento de sua participação de mercado na fruticultura atual. Portanto, com o objetivo de produzir materiais de plantio de boa qualidade, foi realizado um estudo visando otimizar a técnica de propagação e enraizamento adventício e a sobrevivência da camada aérea da cera de macieira. Neste estudo, quatro diferentes níveis de concentração de ácido indol-3-butírico (AIB) (0, 1000, 1500 e 2000 mg L-1) e três meios de enraizamento (musgo esfagno, vermicomposto e solo de jardim) foram aplicados após a remoção da casca (floema) na parte aérea para determinar o efeito no enraizamento e capacidade de sobrevivência da camada de ar da macieira em condições de campo. Os resultados mostraram que os brotos de macieira tratados com 2000 mg L-1 de AIB produziram significativamente maior número de raízes, maior comprimento de raiz, diâmetro de galho, comprimento de galho, número de folhas e área foliar das camadas aéreas. Além disso, o maior teor de clorofila e abertura estomática foram registrados no tratamento 2000 mg L-1 IBA em comparação com outros tratamentos, incluindo o controle. O meio de vermicomposto foi melhor do que o solo de jardim e o musgo esfagno em relação ao enraizamento e capacidade de sobrevivência das camadas aéreas. Os resultados mostraram que a combinação de 2000 mg L-1 de AIB e vermicomposto como meio de enraizamento proporciona a melhor combinação para iniciação radicular, número de raízes, comprimento radicular e taxa de sobrevivência (100%) das camadas aéreas de macieira. A partir deste estudo, pode-se concluir que 2.000 mg L-1 de AIB e tratamento com vermicomposto melhoram a iniciação radicular, o estabelecimento precoce e a capacidade de sobrevivência de macieiras em camadas de ar em condições de campo.
Assuntos
Produção Agrícola , Raízes de Plantas/crescimento & desenvolvimento , Malus/crescimento & desenvolvimento , FertilizantesRESUMO
BACKGROUND AND AIM: Phytoestrogens are traditionally used for cardiovascular risks but direct effects on the ischemic heart remain unclear. Plants with phytoestrogens are used for reducing menopausic symptoms and they could also be cardioprotectives. Here we investigated whether maca (Lepidium meyenii) contains isoflavones and prevents cardiac stunning, in comparison to soy isoflavones. EXPERIMENTAL PROCEDURE: Both products were orally and daily administered to rats during 1 week before exposing isolated hearts to ischemia/reperfusion (I/R). Young male (YM), female (YF) and aged female (AgF) rats treated with maca (MACA, 1 g/kg/day) or soy isoflavones (ISOF, 100 mg/kg/day) were compared to acute daidzein (DAZ, 5 mg/kg i.p.) and non-treated rat groups. Isolated ventricles were perfused inside a calorimeter to simultaneously measure contractile and calorimetrical signals before and during I/R. RESULTS AND CONCLUSIONS: Maca has genistein and daidzein. MACA and ISOF improved the post-ischemic contractile recovery (PICR) and muscle economy (P/Ht) in YM and YF hearts, but not in AgF hearts. DAZ improved PICR and P/Ht more in YM than in YF. The mKATP channels blockade reduced both PICR and P/Ht in DAZ-treated YM hearts, without affecting them in ISOF or MACA-treated YM hearts. In MACA treated YF hearts, the simultaneous blockade of NOS and mKATP channels, or the mNCX blockade reduced cardioprotection. Results show that subacute oral treatment with maca or with soy isoflavones was strongly preventive of cardiac ischemic dysfunction, more than the acute administration of a pure isoflavone (daidzein, genistein). Maca induced synergistic and complex mechanisms which prevented mitochondrial calcium overload.
RESUMO
Based on the apple acreage and output data of 23 provinces in China, the LMDI decomposition method and the barycenter analysis model were used to systematically analyze the spatiotemporal characteristics of apple production in China from 1978 to 2016. The results showed that the apple acreage and output continued to increase, and the apple production layout has moved to south-westward; Shaanxi, Shandong, Henan, Shanxi, Gansu, Hebei, Liaoning, and Xinjiang were the main contributors to the apple output increase in China; yield contributed more to apple output increase, the increase of yield was a significant contributing factor to the apple output growth in 17 provinces, whereas the expansion of apple acreage was a significant contributing factor in the other 6 provinces; the barycenter of apple acreage and output respectively moved to the southwest by 506.63 kilometers and 574.12 kilometers, and the barycenter of apple production gradually shifted to the Loess Plateau. To stabilize the effective supply of apple and maintain industrial security, the policymakers should attach importance to the fundamental role of technological progress in the development of the apple industry, and bring into play the technological progress, economic, social, and environmental effects of apple industry agglomeration by optimizing the apple production layout and strengthening policy guidance and regulatory measures.(AU)
Com base nos dados de área cultivada e produção de maçã de 23 províncias na China, o método de decomposição do LMDI e o modelo de análise de barcenter foram utilizados para analisar sistematicamente as características espaço-temporais da produção de maçã na China entre 1978 e 2016. Os resultados mostraram que a área cultivada de maçã e a produção continuou a aumentar, e o layout da produção da maçã mudou para o Sudoeste; Shaanxi, Shandong, Henan, Shanxi, Gansu, Hebei, Liaoning e Xinjiang foram os principais contribuintes para o aumento da produção de maçãs na China; o rendimento contribuiu mais para o aumento da produção de maçã, o aumento do rendimento foi fator de contribuição significativo para o crescimento da produção de maçã em 17 províncias, enquanto a expansão da área cultivada de maçã foi fator de contribuição significativo nas outras 6 províncias; o baricentro da área cultivada e a produção de maçãs se deslocaram para o Sudoeste em 506,63 quilômetros e 574,12 quilômetros respectivamente. O baricentro da produção de maçãs mudou gradualmente para o platô de Loess. Para estabilizar o fornecimento efetivo de maçã e manter a segurança industrial, os formuladores de políticas devem atribuir importância ao papel fundamental do progresso tecnológico no desenvolvimento da indústria da maçã e colocar em jogo o progresso tecnológico, os efeitos econômicos, sociais e ambientais da indústria da maçã aglomeração, otimizando o layout da produção da maçã e fortalecendo a orientação política e as medidas regulatórias.(AU)
Assuntos
Frutas , Abastecimento de Alimentos/métodos , Interpretação Estatística de Dados , TecnologiaRESUMO
ABSTRACT: Based on the apple acreage and output data of 23 provinces in China, the LMDI decomposition method and the barycenter analysis model were used to systematically analyze the spatiotemporal characteristics of apple production in China from 1978 to 2016. The results showed that the apple acreage and output continued to increase, and the apple production layout has moved to south-westward; Shaanxi, Shandong, Henan, Shanxi, Gansu, Hebei, Liaoning, and Xinjiang were the main contributors to the apple output increase in China; yield contributed more to apple output increase, the increase of yield was a significant contributing factor to the apple output growth in 17 provinces, whereas the expansion of apple acreage was a significant contributing factor in the other 6 provinces; the barycenter of apple acreage and output respectively moved to the southwest by 506.63 kilometers and 574.12 kilometers, and the barycenter of apple production gradually shifted to the Loess Plateau. To stabilize the effective supply of apple and maintain industrial security, the policymakers should attach importance to the fundamental role of technological progress in the development of the apple industry, and bring into play the technological progress, economic, social, and environmental effects of apple industry agglomeration by optimizing the apple production layout and strengthening policy guidance and regulatory measures.
RESUMO: Com base nos dados de área cultivada e produção de maçã de 23 províncias na China, o método de decomposição do LMDI e o modelo de análise de barcenter foram utilizados para analisar sistematicamente as características espaço-temporais da produção de maçã na China entre 1978 e 2016. Os resultados mostraram que a área cultivada de maçã e a produção continuou a aumentar, e o layout da produção da maçã mudou para o Sudoeste; Shaanxi, Shandong, Henan, Shanxi, Gansu, Hebei, Liaoning e Xinjiang foram os principais contribuintes para o aumento da produção de maçãs na China; o rendimento contribuiu mais para o aumento da produção de maçã, o aumento do rendimento foi fator de contribuição significativo para o crescimento da produção de maçã em 17 províncias, enquanto a expansão da área cultivada de maçã foi fator de contribuição significativo nas outras 6 províncias; o baricentro da área cultivada e a produção de maçãs se deslocaram para o Sudoeste em 506,63 quilômetros e 574,12 quilômetros respectivamente. O baricentro da produção de maçãs mudou gradualmente para o platô de Loess. Para estabilizar o fornecimento efetivo de maçã e manter a segurança industrial, os formuladores de políticas devem atribuir importância ao papel fundamental do progresso tecnológico no desenvolvimento da indústria da maçã e colocar em jogo o progresso tecnológico, os efeitos econômicos, sociais e ambientais da indústria da maçã aglomeração, otimizando o layout da produção da maçã e fortalecendo a orientação política e as medidas regulatórias.
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Postharvest processing of maca (Lepidium meyenii Walp., Brassicaceae), a traditional high-altitude Andean root crop, involves slow field drying prior to milling into flour. The progressive tissue dehydration and release of hydrolytic enzymes and substrates from cellular compartments results in the slow accumulation of free monosaccharides, fatty acids and amino acids. A more complex, and faster, kinetic profile is that of glucosinolate breakdown. A number of reactive transient and stable accumulation products are generated during drying, some of which have noteworthy bioactive properties. Among these are macamides, inhibitors of endocannabinoid neurotransmitter degradation in mammalian nervous systems. They result from the condensation of benzyl amine, a glucosinolate hydrolysis product, with free fatty acids released from lipid hydrolysis. Recent research has focused on developing drying processes under controlled conditions that can modulate the biochemistry of glucosinolate hydrolysis to optimize the content of bioactive compounds in the root flour. Low temperature (35 °C) oven-drying of shredded maca roots under controlled air flow generates benzyl amine as primary accumulation product, accounting for up to 94% of hydrolyzed glucosinolate in the flour. Kinetic evidence suggests that both deaminated benzenoids and macamides are allocated from the benzylamine pool through amine oxidase activity or condensation with free fatty acids, accounting for the remaining hydrolyzed glucosinolate (<5%). These activities determine the allocation to either one of these pathways. Later stages of dehydration result in shifts in the molar ratios of deaminated benzenoids, the accumulation of benzoic acid esters and benzyl alcohol. We propose that these are the result of changes in the rates of the reductive and oxidative half-reactions of endogenous aldehyde dehydrogenases. It is the ratio of benzylamine deamination to amide formation that determines the eventual yields of macamides in relation to benzenoids and their esters in maca flour.
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Lepidium , Animais , Dessecação , Farinha , Glucosinolatos , Extratos VegetaisRESUMO
Maca (Lepidium meyenii) is a herbaceous plant grown at over 4,000 m in Peru. It has been studied worldwide for its properties on fertility. Previous studies have assessed maca effects on semen quality, but there is need of randomised, double-blind trials in order to make clinical decisions. The objective of this study was to assess the effect of maca on seminal parameters in infertile adult men. This is a double-blind, randomised, placebo-controlled pilot trial in which sixty-nine patients diagnosed with mild asthenozoospermia and/or mild oligozoospermia were supplied by maca (n = 35) or placebo (n = 34) (2 g/day) for a period of 12 weeks. When compared patients treated with maca and patients treated with placebo, there were no significant differences in semen volume (2.95 ± 0.52 vs. 2.90 ± 0.52; p = .392), sperm motility (22.34 ± 2.22 vs. 23.05 ± 2.22; p = .462) and normal sperm morphology (7.89 ± 1.89 vs. 7.04 ± 2.28; p = .801), but there was a significant difference in sperm concentration (15.04 ± 5.61 vs. 10.16 ± 3.59, respectively; p = .011). In conclusion, patients treated with 2 g of maca for a period of 12 weeks showed a significant improvement in seminal concentration compared with patients treated with placebo. There were no significant differences in semen volume, sperm mobility and morphology when compared both groups.
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Lepidium , Adulto , Humanos , Masculino , Peru , Extratos Vegetais/uso terapêutico , Análise do Sêmen , Contagem de Espermatozoides , Motilidade dos EspermatozoidesRESUMO
ABSTRACT This study describes the development of mixed flour added of maca for gluten-free bread (GFB) employing a Simplex Centroid Design. Four pseudo-components were evaluated in order to improve the GFB specific volume and texture profile analysis: maca (MC; 0.25 to 0.75), potato starch (PS; 0.25 to 0.75), sweet manioc flour (SEM; 0 to 0.25), and sour manioc flour (SOM; 0 to 0.25). The best GFB formulation - by desirability results - was evaluated regarding physio-chemical, microbiological aspects, and sensory acceptance. The responses specific volume and hardness were adjusted with a special cubic model (R2= 0.973 and 0.913), while cohesiveness, springiness and chewiness was adjusted with a linear model. The significant contribution of maca into GFB dough was evident in the models, and the desirability results indicated an appropriate mixed flour composed by MC (0.25), PS (0.5), and SEM (0.25). The GFB elaborated with this mixed flour generated a product well accepted (acceptability index higher than 73.9%) for attributes of aroma, colour, texture, flavour, and overall acceptance. The values of physio-chemical parameters obtained in the present article was in the range described for GFB in the literature. In addition, the processing method applied allowed for obtaining a GFB that was microbiologically stable.
RESUMEN Este estudio describe el desarrollo de la harina mezclada (MF) agregada de maca para la elaboración de pan sin gluten (GFB) empleando un Diseño Centroide Simplex. Se evaluaron cuatro pseudo-componentes para mejorar el volumen específico y el perfil de textura de GFB: maca (MC; 0,25 a 0,75), almidón de patata (PS; 0,25 a 0,75), harina de yuca dulce (SEM; 0 a 0,25), y harina de yuca agrio (SOM; 0 a 0,25). La mejor formulación de GFB - por la deseabilidad - se evaluó en relación con los aspectos fisicoquímicos, microbiológicos, y sensoriales. El volumen específico y la dureza se ajustaron a un modelo cúbico especial (R2= 0,975 y 0,913). La cohesión, elasticidad y masticabilidad se ajustaron a un modelo lineal. La contribución de la maca a la masa de GFB fue evidente en los modelos, y los resultados indican una MF apropiada compuesta por MC (0,25), PS (0.5) y SEM (0,25). El GFB elaborado con esta MF genera un producto aceptado (índice de aceptabilidad mayor que 73,9%) para aroma, color, textura, sabor y aceptación general. Los parámetros físico-químicos obtenidos estaban en el rango descrito para GFB en la literatura y GFB se mostró microbiológicamente estable.
Assuntos
Pão/análise , Doença Celíaca , Lepidium , Dieta Livre de Glúten , Paladar , Solanum tuberosum , Pão/microbiologia , ManihotRESUMO
Benign prostatic hyperplasia (BPH) is a pathology characterised by an increase in prostate size associated with low urinary tract symptoms. Finasteride (F), a 5a-reductase inhibitor, is the standard treatment for BPH reducing prostate weight but also sexual desire. The Peruvian plant known as Red Maca (RM) (Lepidium meyenii) inhibits BPH in rats and mice. The aim of the study was to assess the inflammatory effect of RM and finasteride in rats with testosterone enanthate (TE)-induced BPH. Thirty rats were divided into 5 groups: Control, TE (50 mg/rat), TE + F (0.6 mg/kg), and two groups of TE + RM 40/80 (40 or 80 mg). After treatments, tumour necrosis factor alpha (TNFa), interleukin 4 (IL4) and interferon gamma (INFg) as well as testosterone and oestradiol were evaluated and inflammatory cells (neutrophils, mast cells and lymphocytes) in prostate were quantified. Red Maca and finasteride treatments decreased inflammatory cells counts in prostate, inhibiting TNFa by different pathways. Finasteride increased IL4 whereas Red Maca increased INFg. In conclusion, data suggest that finasteride acts on Th2 response by increasing IL4 in prostate, while Red Maca acts on Th1 response mediated by INFg.
Assuntos
Lepidium/química , Extratos Vegetais/farmacologia , Próstata/efeitos dos fármacos , Hiperplasia Prostática/tratamento farmacológico , Transdução de Sinais/efeitos dos fármacos , Inibidores de 5-alfa Redutase/farmacologia , Inibidores de 5-alfa Redutase/uso terapêutico , Animais , Modelos Animais de Doenças , Finasterida/farmacologia , Finasterida/uso terapêutico , Humanos , Interferon gama/metabolismo , Interleucina-4/metabolismo , Masculino , Extratos Vegetais/uso terapêutico , Próstata/citologia , Próstata/imunologia , Próstata/patologia , Hiperplasia Prostática/induzido quimicamente , Hiperplasia Prostática/imunologia , Hiperplasia Prostática/patologia , Ratos , Transdução de Sinais/imunologia , Testosterona/análogos & derivados , Testosterona/toxicidade , Células Th1/efeitos dos fármacos , Células Th1/imunologia , Células Th1/metabolismo , Células Th2/efeitos dos fármacos , Células Th2/imunologia , Células Th2/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Maca has shown broad application prospects as a new food resource. The function of maca is directly affected by its geographical origin, and an effective method for studying traceability must be established. This study aimed to discriminate maca from different regions at large scales (Peru and China) and small scales (Yunnan, Xinjiang, and Tibet in China) through geographical authentication using the stable isotope ratio (SIR) (δ13C, δ15N, δ2H and δ18O) and mineral elemental fingerprints combined with chemometrics. For Peruvian and Chinese maca, overall discriminatory accuracy of 100% and 96.2% were obtained by applying SIR and the mineral element analysis, respectively. For maca obtained from different Chinese regions, the order of the discriminatory accuracy was mineral elements (80.2%) = SIR combined with mineral elements (80.2%) > SIR (71.9%). K, B, Mn, Fe, Mo, Cd and As were identified as the main discriminating indicators for identifying maca from different Chinese regions.
Assuntos
Lepidium/química , Minerais/análise , China , Geografia , Isótopos/análise , PeruRESUMO
Maca( Lepidium meyenii) known as the " national treasure of Peru" and " South American ginseng",is annual or biennial herbs of the genus Lepidium in Cruciferae. It mainly contains proteins,amino acids,polysaccharides,alkaloids( including:macamides,imidazoles,hydroxypyridines,carbazoles,organic amines and so on),glucosinolates,macaenes,thioethylurea,sterols and other chemical constituents. In recent years,more and more studies have found that it could treat osteoporosis and improve prostatehyperplasia,and possessed anti-cancer,female climacteric syndrome,rheumatism,antioxidant and other pharmacological effects. In this paper,the chemical constituents and bioactivity of Maca were reviewed,which could provide the basis for the further development and utilization of Maca.
Assuntos
Asteraceae , Lepidium , Extratos Vegetais , Antioxidantes , PeruRESUMO
ABSTRACT Macamides and Macaenes are the bioactive marker compounds in maca (Lepidium meyenii Walp., Brassicaceae) tuber. To simultaneously quantify these two types of compound, HPLC method was studied. To distinguish and group the growing regions of different maca samples, Hierarchical cluster analysis, a chemometric method, was applied to analyze the HPLC data. The calibration curves obtained using the HPLC method showed satisfactory linearity with determination coefficients >0.9998. The precision and repeatability relative standard deviation values were <4%, and the accuracy relative standard deviation value was <5%. The limits of detection was <0.1 µg/ml and the limit of quantification was <0.3 µg/ml. Our HPLC method was successfully used for the separation and determination of macamides and macaenes in Maca within 45 min, i.e., two macaenes (9-oxo-10E,12Z-octadecadienoic acid and 9-oxo-10E,12E-octadecadienoic acid) and five macamides (N-benzyl-9-oxo-10E,12Z-octadecadienamide, N-benzyl-9-oxo-10E,12E-octadecadienamide, N-benzyl-9Z,12Z,15Z-octadecatrienamide, N-benzyl-9Z,12Z-octadecadienamide and N-benzyl-hexadecanamide). The HPLC method was applied to analyze and quantify the seven compounds in thirty maca samples with different colors and origins. The origins of all the maca samples were distinguished and grouped using hierarchical cluster analysis of the HPLC data. Accordingly, the metabolism of macaenes and macamides in maca post-harvest processing has also been proposed. The HPLC method is efficient to simultaneously quantify the macamides and macaenes in maca. Analyzing the HPLC data using hierarchical cluster analysis can distinguish maca growing origins.
RESUMO
Lepidium meyenii is widely used as a food supplement because of its medicinal properties and nutritional value. Several studies have described the identification of its metabolites and LC-HRMS analysis is one of the most frequently used analytical tool to assess plant extract metabolome. Nevertheless, proper and reliable metabolite identification is essential for such metabolomics studies. Although HRMS distinguishes even minor changes in the metabolites structures, highly specialized secondary metabolites might not be present on available databases imposing serious obstacles for metabolite identification This review discusses the structural diversity and biosynthetic aspects of 101 compounds previously identified in L. meyenii. More importantly, we have compiled the available information on expected and experimental high-resolution masses of L. meyenii compounds. This will help upcoming metabolomics studies not only of L. meyenii but also other plant species by providing the necessary tools to perform proper and reliable identification of their bioactive compounds.
Assuntos
Lepidium/química , Compostos Fitoquímicos/biossíntese , Compostos Fitoquímicos/química , Extratos Vegetais/química , Metaboloma , Metabolômica , Estrutura Molecular , Valor Nutritivo , Metabolismo SecundárioRESUMO
This study aimed to describe and discuss the results of an experiment carried out in two stages with pregnant cows fed 25kg/apple pomace/day. The first stage involved 16 pregnant Holstein Friesian cows divided into four groups: Group 0 - Control (5 cows); Group I - 1 month-gestation (4 cows); Group II - 3 month-gestation (4 cows); Group III - 6 month-gestation (3 cows) and was performed from September to December 2015. The second stage comprised 12 pregnant Holstein Friesian cows divided into three groups: Group 0 - Control (6 cows), Group I - 1 month-gestation (3 cows), and Group II - 3 month-gestation (3 cows) and was conducted from April 2016 to February 2017. All study animals received apple pomace at a dose of 25kg/day. As for the first experiment stage, a cow in Group III bred a calf with complete absence of the coccygeal vertebrae and tail, slight bending of the hind limbs, scoliosis in the thoracic spine, and limited mobility. At 30 days, it presented with diarrhea and underdevelopment, and was euthanized for necropsy. At gross examination, malformations were observed in the thoracic spine, coxofemural joint, and genitourinary tract. Regarding the second experiment stage, a cow in Group I gave birth to a calf with curved pelvic and thoracic limbs with thick joints and flattening hooves. Microscopic examination of the femur showed disorganized, irregular hypertrophic zone and scarce growth zone, in addition to primary spongy zone with short, slightly mineralized trabeculae. Samples of the apple pomace used in this study were frozen and sent for laboratory evaluation of pesticide residues, which showed a positive result for the fungicide carbendazim.(AU)
O presente trabalho tem por finalidade descrever e discutir os resultados do experimento realizado em vacas prenhes que foram alimentadas com 25kg/dia de bagaço de maçã. Experimentos foram conduzidos em duas etapas, a primeira no ano de 2015, de setembro a dezembro onde foram utilizadas 16 vacas prenhes da raça holandês. Estas foram divididas em quatro grupos: Grupo 0, Controle (5 vacas); Grupo I, 1 mês gestação (4 vacas); Grupo II, 3 meses gestação (4 vacas); Grupo III, 6 meses gestação (3 vacas). A segunda etapa foi realizada em abril de 2016 a fevereiro de 2017. Foram utilizadas 12 vacas prenhes da raça holandês, divididas em três grupos: Grupo 0, Controle (6 vacas); Grupo I, 1 mês gestação (3 vacas); Grupo II, 3 meses gestação (3 vacas). Todas as vacas receberam bagaço de maçã na dose de 25kg/dia/vaca. Para o primeiro experimento, uma vaca do Grupo III pariu uma bezerra, com ausência completa das vértebras coccígeas e cauda, encurvamento leve dos membros posteriores, escoliose na coluna torácica e dificuldade de locomoção. Decorridos 30 dias do nascimento, manifestou diarreia e pouco desenvolvimento, sendo eutanasiada para necropsia. Na macroscopia, havia malformações na coluna torácica, articulação coxofemoral e no aparelho urogenital. Em relação ao segundo experimento uma vaca do Grupo I pariu uma bezerra com membros pélvicos e torácicos, curvos e com articulações consideravelmente grossas e "achinelamento" de cascos. Na microscopia do fêmur foi observado na placa epifisária, zona hipertrófica desorganizada, irregular e zona de crescimento escassa. Na zona esponjosa primária observou-se trabéculas curtas e pouco mineralizadas. Amostras do bagaço de maçã utilizado na experimentação foram congeladas e enviadas para avaliação de resíduos agrotóxicos, onde foi encontrado resultado positivo para o fungicida carbendazim.(AU)