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1.
Biochem Biophys Rep ; 40: 101831, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39398538

RESUMO

Obesity is a major public health problem and is a major contributor to the development of insulin resistance. In previous studies we observed that single-wavelength red or infrared photobiomodulation (PBM) improved insulin signaling in adipocytes and skeletal muscle of mice fed a high-fat diet, but information about the combination of different wavelengths, as well as the effect of different light doses (J/cm2) is lacking. Therefore, the aim of this study was to investigate the effects of different doses of dual-wavelength PBM on insulin signaling in muscle cell, and explore potential mechanisms involved. Mouse myoblasts (C2C12) were differentiated into myotubes and cultured in palmitic acid, sodium oleate and l-carnitine (PAL) to induce insulin resistance high or in glucose medium (CTRL). Then, they received SHAM treatment (lights off, 0 J/cm2) or PBM (660 + 850 nm; 2, 4 or 8 J/cm2). PAL induced insulin resistance (assessed by Akt phosphorylation at ser473), attenuated maximal citrate synthase activity, and increased the phosphorylation of c-Jun NH(2) terminal kinase (JNK) (T183/Y185). PBM at doses of 4 or 8 J/cm2 reversed these PAL-induced responses. Furthermore, at doses of 2, 4 or 8 J/cm2, PBM reversed the increase in mitofusin-2 content induced by PAL. In conclusion, the combination of dual-wavelength red and infrared PBM at doses of 4 and 8 J/cm2 improved intracellular insulin signaling in musculoskeletal cells, and this effect appears to involve the modulation of mitochondrial function and the attenuation of the activation of stress kinases.

2.
Water Res ; 266: 122322, 2024 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-39213680

RESUMO

Ultraviolet light-emitting diodes (UV-LEDs) have demonstrated the ability to inactivate microorganisms in water, offering an environmentally safer alternative to the conventional mercury lamp, in UV applications. While several studies have explored the microbiological effect of UVC-LEDs (200nm-280nm), limited information exists regarding their effects on waters with critical qualities. These critical qualities encompass bacteria, viruses, and protozoa - drinking water quality indicators defined by the World Health Organization for small water systems. For the first time, this work reports on the Escherichia coli, PhiX-174, MS2, and Cryptosporidium oocysts inactivation using a bench-scale UVC-LED (280 nm) water disinfection system. UV doses at a wavelength of 280 nm (UV280) of up to 143.4 mJ/cm2 were delivered under two quality-critical water conditions: filtered water (UV transmittance at 280 nm - UVT280 90.2 %) and WHO challenge water (UVT 15.7 %). Results revealed microbiological reductions dependent on exposure time and UVT. For UV280 dose of 16.1 mJ/cm2, 2.93-3.70 log E. coli reductions were observed in UVT 90.2 % and 15.7 %, 3.49-4.21 log for PhiX-174, 0.63-0.78 log for MS2, and 0.02-0.04 log for Cryptosporidium oocysts. Significantly higher UV280 doses of 143.4 mJ/cm2 led to reductions of 3.94-5.35 log for MS2 and 0.42-0.46 log for Cryptosporidium oocysts. Statistical analysis revealed that the sensitivity among the organisms to UV280 exposure was E. coli = PhiX-174 > MS2 >> Cryptosporidium oocysts. Although experiments with WHO challenge water posed greater challenges for achieving 1 log reduction compared to filtered water, this difference only proved statistically significant for PhiX-174 and MS2 reductions. Overall, UVC-LED technology demonstrated notable efficacy in microbiological inactivation, achieving significant reductions based on WHO scheme of evaluation for POU technologies in both bacteria and viruses even in critical-quality waters. The findings emphasize the potential for extending the application of UVC-LED as a viable solution for household water treatment.

3.
Photochem Photobiol ; 2024 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-38888236

RESUMO

This research investigated the duration of the influence of red light-emitting diodes (LED, 630 nm; output power: 2452.5 mW; laser beam: 163.5 cm2; irradiance: 15 mW/cm2; radiant exposure: 4 J/cm2) on different periods after irradiation (6, 12, 24, 48, and 72 h) on adipose-derived mesenchymal stem cells' (AdMSCs) metabolism and paracrine factors. AdMSCs were irradiated three times every 48 h. Twenty-four hours after the last irradiation, there was a higher MTT absorbance, followed by a decrease after 48 h. The cells' secretome showed increased levels of IL-6 and VEGF after 12 and 24 h, but this was reversed after 48 h. Additionally, LED irradiation resulted in higher levels of nitrite and did not affect oxidative stress markers. LED irradiation had significant effects on AdMSCs after 24 h compared to other groups and its control group.

4.
J Conserv Dent Endod ; 27(4): 408-413, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38779204

RESUMO

Aims: The objective of the study was to evaluate two translucent resin composite systems for customizing light-polymerized fiber posts with light-emitting diode (LED) curing units regarding adhesion using conventional cement at 24 h and 6 months. Settings and Design: This was an experimental in vitro study. Methods: Forty roots were prepared and divided into four groups (n = 10): ZV-Z350 resin and LED Valo; ZR-Z350 resin and LED Radii-Cal; OV-Opallis resin and LED Valo; and OR-Opallis resin and LED Radii-Cal. The fiber post was customized and cemented with conventional resin cement and was photoactivated by two different sources. Statistical Analysis Used: The data were subjected to two-way ANOVA and Tukey's post hoc tests (P = 0.05). The data regarding the pattern of adhesive failures were described in terms of the frequency of occurrence in each third of the postspace. Results: Both at 24 h and 6 months, bond strength among groups was similar, regardless of the analyzed postspace 3rd (P > 0.05). Adhesive failure Type 4 was the most frequent in all thirds. Conclusions: Customizing the fiber post with Z350 and Opallis has the same effect on bond strength and adhesive failure pattern, regardless of the LED curing units used for photopolymerization.

5.
Parasitol Res ; 123(4): 194, 2024 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-38656453

RESUMO

Light-Emitting Diodes (LEDs) have been effective light sources in attracting Anopheles mosquitoes, but the broad-spectrum white light, even with a wide-ranging application in lighting, have not been evaluated yet. In this study, the white light was field evaluated against the green one in the light trapping of anopheline mosquitoes by using two non-suction Silva traps and two CDC-type suction light traps. Anopheline mosquitoes were captured for two 21-night periods of collecting (2022 and 2023). In the first period, two LEDs were used per Silva trap, but three were used in the second one to increase the luminance/illuminance at traps. A CDC-type suction light trap equipped with an incandescent lamp was used in 2022 and a CDC-type suction light trap equipped with a 6 V-white light (higher luminance/illuminance) in 2023. A total of eight species and 3,289 specimens were captured in both periods. The most frequent species were Anopheles triannulatus s.l., An. goeldii, An. evansae and An. argyritarsis. In 2022, white LEDs were less attractive to anopheline mosquitoes than the other light sources, but without statistical difference among treatments (F = 2.703; P = 0.0752; df = 2). In 2023, even with an increased luminance/illuminance at traps, no statistical difference was found between the two LED-baited Silva traps (F = 6.690; P = 0.0024; df = 2), but rather between the 6 V-white-baited CDC-type suction light trap and green-baited Silva traps. Due to some drawbacks and the lower abundance of individuals caught by using white LEDs, the narrow-banded green LEDs is preferable to white ones for attracting anophelines.


Assuntos
Anopheles , Luz , Controle de Mosquitos , Animais , Anopheles/fisiologia , Anopheles/efeitos da radiação , Controle de Mosquitos/métodos , Controle de Mosquitos/instrumentação
6.
Lasers Med Sci ; 39(1): 70, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-38378954

RESUMO

Inflammatory bowel diseases (IBD) are chronic and multifactorial diseases characterized by dysfunction of the intestinal mucosa and impaired immune response. Data show an important relationship between intestine and respiratory tract. The treatments of IBD are limited. Photobiomodulation (PBM) is an effective anti-inflammatory therapy. Our objective was to evaluate the repercussion of IBD as well as its treatment with PBM on pulmonary homeostasis. Male Wistar rats were submitted to IBD induction by acetic acid and treated or not with PBM. Rats were irradiated with red LED on both right and left sides of the ventral surface and beside the external anal region during 3 consecutive days (wavelenght 660 nm, power 100 mw, total energy 15 J and time of irradiation 150 s per point). Our results showed that IBD altered pulmonary homeostasis, since we observed an increase in the histopathological score, in myeloperoxidase activity (MPO), in mast cell degranulation, and in the release and gene expression of cytokines. We also showed that PBM treatment reduced biomarkers of IBD and reverted all augmented parameters in the lung, restoring its homeostasis. Thus, we confirm experimentally the important gut-lung axis and the role of PBM as a promising therapy.


Assuntos
Doenças Inflamatórias Intestinais , Terapia com Luz de Baixa Intensidade , Ratos , Masculino , Animais , Ratos Wistar , Terapia com Luz de Baixa Intensidade/métodos , Doenças Inflamatórias Intestinais/radioterapia , Antioxidantes , Pulmão
7.
J Biophotonics ; 17(5): e202300501, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38262071

RESUMO

CONTEXT: Photobiomodulation therapy (PBMT) has been widely used to improve strength, fatigue resistance and increase muscle mass in healthy individuals. These effects could help critically ill patients admitted to intensive care units (ICUs) who show reduced mobility and muscle strength. ICU-acquired weakness lessens overall health and increases the patient's length of stay in the ICU. OBJECTIVE: This study evaluated the effects of PBMT using low intensity light-emitting diodes (LEDs) on the mobility and muscle strength (functional capacity) and length of stay of patients admitted to hospital ICU. METHODS: This randomized, triple-blind, sham-controlled trial was conducted in a hospital ICU. Sixty patients were randomly assigned to two equal groups: (a) PBMT and (b) Sham. PBMT was applied daily to patients until their discharge from the ICU, using a flexible neoprene array of 264 LEDs (120 at 635 nm, 1.2 mW each; 144 at 880 nm, 15 mW each) for 90s (207.36 Joules) at each site. Ten sites were located bilaterally on the thighs, legs, arms, and forearms ventrally and dorsally, 15 min totaling 2,073.6 Joules per session. Outcomes were length of stay (in h) until discharge from the ICU, muscle strength by the Medical Research Council (MRC) score and handgrip dynamometry (HGD), patient mobility by Intensive Care Unit Mobility Scale (IMS) and the Simplified Acute Physiology Score 3 (SAPS 3) for predicting mortality of patients admitted to the ICU. RESULTS: PBMT reduced the average length of stay in the ICU by ~30% (p = 0.028); increased mobility (IMS: 255% vs. 110% p = 0.007), increased muscle strength (MRC: 12% vs. -9% p = 0.001) and HGD (34% vs. -13% p < 0.001), and the SAPS3 score was similar (p > 0.05). CONCLUSION: The results suggest that daily PBMT can reduce the length of stay of ICU patients and increase muscle strength and mobility.


Assuntos
Unidades de Terapia Intensiva , Tempo de Internação , Terapia com Luz de Baixa Intensidade , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Força Muscular , Adulto , Idoso , Músculo Esquelético/fisiologia , Músculo Esquelético/efeitos da radiação
8.
BMC Complement Med Ther ; 24(1): 3, 2024 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-38167061

RESUMO

BACKGROUND: Blue light exposure is known to induce reactive oxygen species (ROS) production and increased endoplasmic reticulum stress, leading to apoptosis of photoreceptors. Maqui berry (Aristotelia chilensis) is a fruit enriched in anthocyanins, known for beneficial biological activities such as antioxidation. In this study, we investigated the effects of Maqui berry extract (MBE) and its constituents on the subcellular damage induced by blue light irradiation in mouse retina-derived 661W cells. METHODS: We evaluated the effects of MBE and its main delphinidins, delphinidin 3-O-sambubioside-5-O-glucoside (D3S5G) and delphinidin 3,5-O-diglucoside (D3G5G), on blue light-induced damage on retinal cell line 661W cells. We investigated cell death, the production of ROS, and changes in organelle morphology using fluorescence microscopy. The signaling pathway linked to stress response was evaluated by immunoblotting in the whole cell lysates or nuclear fractions. We also examined the effects of MBE and delphinidins against rotenone-induced mitochondrial dysfunction. RESULTS: Blue light-induced cell death, increased intracellular ROS generation and mitochondrial fragmentation, decreased ATP-production coupled respiration, caused lysosomal membrane permeabilization, and increased ATF4 protein level. Treatment with MBE and its main constituents, delphinidin 3-O-sambubioside-5-O-glucoside and delphinidin 3,5-O-diglucoside, prevented these defects. Furthermore, MBE and delphinidins also protected 661W cells from rotenone-induced cell death. CONCLUSIONS: Maqui berry may be a useful protective agent for photoreceptors against the oxidative damage induced by exposure to blue light.


Assuntos
Antocianinas , Elaeocarpaceae , Animais , Camundongos , Antocianinas/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Frutas , Luz Azul , Rotenona , Elaeocarpaceae/metabolismo , Glucosídeos , Organelas/metabolismo
9.
Clin Ophthalmol ; 18: 215-225, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38283180

RESUMO

Photobiomodulation (PBM) is a type of phototherapy that employs light-emitting diodes (LEDs) or low-power lasers to selectively administer specific wavelengths of visible light, ranging from 500 to 1000 nm, including near-infrared (NIR) wavelengths. LEDs are advantageous compared to lasers due to their ability to treat large areas at a lower cost, lack of tissue damage potential in humans, and reduced risk of eye-related accidents. The ophthalmology community has recently taken interest in PBM as a promising novel approach for managing various retinal conditions such as age-related macular degeneration, retinopathy of prematurity, retinitis pigmentosa, diabetic retinopathy, Leber's hereditary optic neuropathy, amblyopia, methanol-induced retinal damage, and potentially others. This review critically assesses the existing body of research on PBM applications in the retina, focusing on elucidating the underlying mechanisms of action and evaluating the clinical outcomes associated with this therapeutic modality.

10.
Lasers Med Sci ; 39(1): 34, 2024 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-38231300

RESUMO

PURPOSE: Long-term human immunodeficiency virus (HIV)-infected patients are considered at higher risk for osteoporosis. Among the various causes that lead these patients to lower bone health, there is the use of antiretroviral drugs (ARVs), especially protease inhibitors (PI), such as ritonavir (RTV). In this context, emerge the potential benefits of LED therapy, whose effects on bone cells are currently being extensively studied, showing a modulation in cell differentiation. However, it remains unclear if photobiostimulation might interfere with RTV effects on osteoblast differentiation. METHODS: In the present study, we investigated the effects of red LED (625 nm) irradiation (15 mW/cm2, 0.2 J/cm2, and 8 mW/cm2, 0.12 J/cm2) on osteoblast cell line MC3T3-E1 treated with RTV (2.5, 5, and 10 µg/mL). RESULTS: Our results indicated that red LED irradiation was able to reverse, or at least minimize, the deleterious effects of RTV on the osteoblasts. Neither the ARV treatments 5 and 10 µg/mL (104.4% and 95.01%) nor the LED protocols (100.3% and 105.7%) statistically altered cell viability, assessed by the MTT assay. Also, the alkaline phosphatase activity and mineralization showed a decrease in osteoblast activity followed by ARV exposure (39.3-73%), which was attenuated by LED in more than 70% with statistical significance (p < 0.05). CONCLUSION: In conclusion, photobiostimulation with red LED at 625 nm was associated with improved beneficial biological effects as a potential inducer of osteogenic activity on RTV-affected cells. This is the first study that investigated the benefits of red LED irradiation over ARV-treated in vitro osteoblasts.


Assuntos
Infecções por HIV , Ritonavir , Humanos , Ritonavir/efeitos adversos , Luz Vermelha , Osteoblastos , Osteócitos , Infecções por HIV/tratamento farmacológico
11.
J Food Sci ; 89(1): 552-565, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38078775

RESUMO

Although ultraviolet-C light-emitting diode (UVC-LED) has proven antimicrobial effectiveness doses needed to reach it cause adverse effects on the physicochemical quality of fish, and thus, optimization studies are crucial to boost its industrial application. This study aimed to identify optimal UVC-LED conditions for maximum shelf life extension with the least possible quality changes of refrigerated stored tilapia fillets from a central composite rotatable design (CCRD). UVC-LED powers (1, 1.38, and 1.58 mW/cm2 ) and times (500, 1800, and 2700 s) were set on the CCRD, which generated 11 treatments, including three replicate experiments. Treatments were analyzed for total aerobic psychrotrophic count, lipid oxidation, instrumental color, and texture parameters on days 0, 2, 4, 7, 11, and 14. The UVC-LED affected shelf life and physicochemical parameters in a nonlinear fashion. UVC-LED-treated fish had increased shelf life by 2.80-4.76 days and increase or decrease in lipid oxidation (0.025-0.276 mg of malondialdehyde [MDA]/kg), total color change (∆E = 3.47-9.06), and hardness (1.31-8.51 N) over the refrigerated storage depending on specific UVC-LED conditions applied. The optimal UVC-LED condition was 0.97 mW/cm2 with 2503.6 s (2428.50 mJ/cm2 ), which increased the fillet's shelf life by 2.5-fold (2 days) while maintaining quality closer to the original throughout refrigerated storage, resulting in ∆E < 5, an increase of only 0.05 mg of MDA/kg, and preservation of the decrease in hardness by 3.38 N compared to its control counterparts. Therefore, it represents an eco-friendly technology that can easily scaled industrially to enhance the sustainable fish production chain. PRACTICAL APPLICATION: The high fish perishability is a global concern due to food safety risks and waste generation impacting the environment adversely, especially nowadays, where fish production and consumption have increased, and there are more evident efforts to sustainable production. UVC-LED is an eco-friendly technology with proven antimicrobial effectiveness but doses needed to reach this effect enhance oxidative degradation. Despite that, optimization studies concerning the maximum shelf life extension while retaining the physicochemical quality of refrigerated stored fish are a gap in the literature and a barrier to its industrial application. Our findings are helpful in sustainably enhancing the fish production chain.


Assuntos
Anti-Infecciosos , Ciclídeos , Animais , Conservação de Alimentos/métodos , Vácuo , Lipídeos
12.
Foodborne Pathog Dis ; 21(3): 168-173, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38090762

RESUMO

Ultraviolet-C light-emitting diode (UVC-LED) and ultrasound (US) are two nonthermal technologies with the potential to destroy pathogens. However, little is known about their effectiveness in strains with a history of heat resistance. Thus, this study aimed to evaluate the phenotype and genotype of heat-resistant extraintestinal pathogenic Escherichia coli (ExPEC) with heat resistance genes after the application of US, UVC-LED, and UVC-LED+US. For this, two central composite rotatable designs were used to optimize the UVC-LED and US conditions in four ExPEC isolated from beef. From the genome of these isolates obtained in a previous study, possible genes for UVC resistance were analyzed. Results showed that US was ineffective in reducing >0.30 log colony-forming unit/mL, and that when used after UVC-LED, it showed a nonsynergic or antagonistic effect. Also, UVC-LED had the greatest effect at the maximum dose (4950 mJ/cm2 from 1.65 mW/cm2 for 50 min). However, the strains showed some recovery after that, which could be implicated in the expression of genes included in SOS system genes, some others present in the transmissible Locus of Stress Tolerance (trxBC and degP), and others (terC). Thus, ExPEC can overcome the conditions used in this study for US, UVC-LED, and UVC-LED+US, probably due to the history of resistance to other cellular damage. The result of this study will contribute to future studies that aim to find better treatment conditions for each food product.


Assuntos
Infecções por Escherichia coli , Escherichia coli Extraintestinal Patogênica , Animais , Bovinos , Escherichia coli Extraintestinal Patogênica/genética , Temperatura Alta , Genótipo , Fenótipo
13.
Parasitol Res ; 123(1): 9, 2023 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-38052759

RESUMO

Phlebotomine sand flies are crepuscular and nocturnal small dipteran insects in the family Psychodidae. Several disease agents, including Leishmania parasites, are transmitted to humans and other vertebrate hosts by the bite of an infected female sand fly. As part of leishmaniasis surveillance programs, light traps have been routinely used in sand fly collections. In this context, new trapping devices are always being required to improve vector monitoring. Here, the efficiency of a new suction light trap, named Silva suction trap or SS trap, was field evaluated in collecting sand flies. Two SS traps, one with green (520 nm, 15,000 mcd) and the other with white (wide spectrum, 18,000 mcd) LEDs, and one CDC-type trap were deployed in a rural forested environment. A total of 4686 phlebotomine sand flies were captured. The most frequent species were females of the Ps. Chagasi series (77.8%) followed by males of Ps. wellcomei (11.6%), Nyssomyia whitmani (3.3%), and Bichromomyia flaviscutellata (2.4%). The CDC-type light trap collected 101.9 ± 20.89 sand flies and 14 species, followed by the white-baited SS trap (87.78 ± 16.36, 14), and the green-baited SS trap (70.61 ± 14.75, 15), but there were no statistically significant differences among traps. A discussion on the considerable advantages of the use of SS traps over CDC traps is included. In this study, the Silva suction trap proved to be efficient and can be an alternative to CDC traps for monitoring adult phlebotomine sand fly populations.


Assuntos
Leishmania , Leishmaniose , Phlebotomus , Psychodidae , Humanos , Masculino , Animais , Feminino , Sucção
14.
Artigo em Inglês | MEDLINE | ID: mdl-37831245

RESUMO

This manuscript critically reviews the state of the art on the application of photo-Fenton processes irradiated by light-emitting diode arrays (LED) with a focus on the removal of contaminants of emerging concern (CEC) from aqueous matrices. LEDs are clean, low-cost radiation sources with longer lifespan compared to mercury lamps. This study covers the influence of LED sources, wavelengths, and dose upon CEC removal, and the potential for disinfection, abatement of antibiotic-resistant bacteria (ARB), and genes (ARG). The bibliographic search was performed in Scopus database using keyword combinations and resulted in a portfolio containing 52 relevant articles published between 2010-2023. According to reviewed papers, LED photoreactor design has evolved in the past decade aiming to improve CEC degradation in aqueous matrices while reducing construction and operation costs, and energy consumption. Among several reactors (annular, fluidized bed, parallel plate, wireless, pathway systems, and microreactor) surveyed for their performance and scalability, LED chips and strips are particularly suitable for application due to their wide emission angle (≈120°) and small size (mm2), which allow for, respectively, efficient illumination coverage and flexible arrangement and design. LED microreactors are very efficient in the degradation of contaminants and scalable with reduced area requirements. Although most studies were performed in synthetic solutions and at laboratory scale, the externally LED irradiated cylindrical reactor was successful for application in full-scale municipal water treatment plants. Future studies should focus on evaluating CEC removal in wastewater using scalable devices for continuous operation of solar photo-Fenton at night.

15.
Lasers Med Sci ; 38(1): 173, 2023 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-37530880

RESUMO

To evaluate the effects of photobiomodulation on the physical performance of healthy women, considering the menstrual cycle. 27 physically active healthy women (age 25.68 ± 3.99 years; mass 63.76 ± 12.77 kg; height 1.65 ± 0.59 cm) during the initial follicular phase (FF1 and FF2) of the menstrual cycle underwent performance evaluations, through a supramaximal test, subjective perception of exertion, blood lactate, and evaluations in the isokinetic dynamometer. Photobiomodulation (PBM) (200J) and Sham (0J) therapy were applied 10 min before the performance evaluations on the quadriceps femoris, hamstrings, and triceps surae muscles. A significance level of 5% was adopted and the effect size was calculated by Cohen's d. It was not possible to observe a significant difference (p > 0.05) in any of the performance variables evaluated in the comparison between groups, only small effects for total distance, final subjective perception of exertion, lactate peak and lactate delta in the PBM group. PBM did not improve muscle performance, resistance to fatigue, perceived exertion, and blood lactate concentrations during a predominantly anaerobic test in healthy women during the FF of the menstrual cycle.


Assuntos
Fase Folicular , Terapia com Luz de Baixa Intensidade , Humanos , Feminino , Adulto Jovem , Adulto , Método Duplo-Cego , Ciclo Menstrual , Ácido Láctico , Desempenho Físico Funcional
16.
Lasers Med Sci ; 38(1): 159, 2023 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-37442837

RESUMO

The aim of this study was to evaluate the influence of IR (λ850 ± 10 nm) and violet (λ405 ± 10 nm) LED phototherapy on total mast cells counts and its ability to influence mast cell degranulation. For this, 27 Wistar rats were used and were randomly distributed into three groups: control, IR LED, and violet LED. When indicated, irradiation done and they were sacrificed, had their tongue removed immediately, 20-min, 45-min, and 2-h after irradiation. Samples were processed to wax, cut, and stained with Toluidine Blue. Intact and degranulated mast cells were counted under light microscopy, and statistical analysis was carried out. In the superficial connective tissue and muscular tissues, violet LED light caused a significant increase in both total number and degranulated mast cells when compared to the control group immediately after irradiation. The degranulation indexes were higher in the groups irradiated with Violet light, both in superficial connective tissue and muscular tissues in relation to the timing. Irradiation with IR LED caused immediate increase in the total number and degranulated of mast cells when compared to the control group only in the superficial connective tissue. In all times observed, the highest total amount of mast cells was seen immediately after irradiation, except in the muscular tissue, which presented the highest amount after 20-min. It was concluded that IR and violet LED light were able to increase the number of mast cells and inducing degranulation in oral mucosa. However, considering that violet LED light can be harmful in periodontal disease, it seems that the use of IR LED light could be the best option in Dentistry.


Assuntos
Degranulação Celular , Mastócitos , Animais , Ratos , Degranulação Celular/efeitos da radiação , Modelos Animais de Doenças , Mastócitos/efeitos da radiação , Fototerapia , Ratos Wistar
17.
Lasers Med Sci ; 38(1): 163, 2023 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-37464155

RESUMO

To review the effects of photobiomodulation therapy (PBMT) on pain intensity and disability in people with plantar fasciitis (PF) when compared with control conditions, other interventions, and adjunct therapies. Systematic searches were conducted in five database randomized controlled trials (RCT). We only included randomized controlled trials (RCTs) in adults with PF that compared PBMT to placebo, as well as RCTs that compared PBMT to other interventions; and as an adjunct to other therapies. The methodological quality and certainty were assessed through PEDro Scale and GRADE approach, respectively. The data of comparison were pooled and a meta-analysis was conducted when possible. Nineteen RCTs involving 1089 participants were included in this review. PBMT alone (MD = - 22.02 [- 35.21 to - 8.83]) or with exercise (MD = - 21.84 [- 26.14 to - 17.54]) improved pain intensity in short-term treatment. PBMT was superior to (extracorporeal shock wave therapy) EWST for relief of pain (MD = - 20.94 [- 32.74 to - 9.13]). In the follow-up, PBMT plus exercise had a superior to exercise therapy alone (MD = - 18.42 [- 26.48 to - 10.36]). PBMT may be superior to (ultrasound therapeutic) UST in medium- and long-term follow-ups for disability, but can be not clinically relevant. There is uncertainty that PBMT is capable of promoting improvement in disability. PBMT when used with adjuvant therapy does not enhance outcomes of interest. PBMT improves pain intensity with or without exercise. PBMT has been shown to be superior to ESWT for pain relief, but not superior to other interventions for pain intensity and disability. The evidence does not support PBMT as an adjunct to other electrotherapeutic modalities.


Assuntos
Tratamento por Ondas de Choque Extracorpóreas , Fasciíte Plantar , Terapia com Luz de Baixa Intensidade , Adulto , Humanos , Medição da Dor , Fasciíte Plantar/radioterapia , Dor/tratamento farmacológico
18.
Photobiomodul Photomed Laser Surg ; 41(6): 277-282, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37335619

RESUMO

Objective: This study analyzed the histological and immunohistochemical changes in hair follicles submitted to epilation with light-emitting diode (LED). Background: The use of specific wavelengths of LED leads to the absorption of photons by chromophore tissues, enabling different photophysical and photochemical events, bringing therapeutic benefits such as removing body hair. Methods: The sample included five participants, with phototypes II-V, divided into two groups. The volunteers received a session of epilation with the Holonyak® device on the pubic region and right groin, whereas the contralateral side was kept as a control. An energy of 10 J and a cooling temperature of -5°C were used, after which the pain provoked by the equipment was questioned using the analogue pain scale. After 45 days, the punching procedure was performed in the region where skin samples were taken for histological and immunohistochemical analysis. Results: For all phototypes, in the treated area, the follicles and sebaceous glands were in a stage of involution, showing perifollicular inflammatory infiltrate with changes suggestive of apoptosis. The apoptosis process was confirmed by the increase in markers cytokeratin-18 and cleaved caspase 3, in addition to the reduced expression of Blc-2, and the lower cell proliferation (Ki67), reinforcing the action of LED based on the definite involution and resorption of the follicle, through macrophages (CD68) triggered by the inflammatory process. Conclusions: The preliminary results of this study found relevant histological changes and immunohistochemical markers in the epilation process, which may indicate the efficacy of LED in permanent hair removal.


Assuntos
Remoção de Cabelo , Humanos , Remoção de Cabelo/métodos , Projetos Piloto , Pele , Folículo Piloso
19.
Lasers Med Sci ; 38(1): 85, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36920639

RESUMO

To evaluate the effects of Light-Emitting Diode (LED) irradiation on the expression of thermogenesis and lipogenesis-associated markers in adipose tissue and metabolic parameters of obese mice. Twenty-four male mice were divided into four groups: i) ST fed standard diet; ii) HCD fed hyperglycemic diet; iii) LED + I fed hyperglycemic diet and irradiated with LED in the interscapular region; iv) LED + A fed hyperglycemic diet and irradiated with LED in the abdominal region. The first phase of the study comprehended the induction of obesity for 12 weeks. Next, the animals were submitted to six irradiation sessions (days 1, 3, 7, 10, 14, and 21) using a 660-nm LED (5.77 J/cm2 at 48,1 mW/cm2). Anthropometric, biochemical, and histological parameters and the expression of thermogenesis and lipogenesis-associated markers were assessed in adipose tissue. There was diminished weight gain between the HCD and LED + A groups (ST: 0.37 ± 0.65; HCD: 3.10 ± 0.89; LED + I: -1.26 ± 0.83; LED + A: -2.07 ± 1.27 g; p < 0.018). There was a 33.3% and 23.8% reduction in epidydimal adipose tissue weight and a 25% and 10.7% in the visceral adiposity for the LED + I and LED + A groups, respectively, when compared with HCD. There was a decreased accumulation of fat droplets in adipose tissue in LED + A and LED + I groups. Additionally, LED irradiation was associated with increased mRNA expression of uncoupling protein 1 (UCP1) in the brown adipose tissue (ST: 2.27 ± 0.19; HCD: 1.54 ± 0.12; LED + I: 2.44 ± 0.22; p = 0.014) and decreased fatty acid synthetase (FAS) expression in epidydimal adipose tissue (ST: 0.79 ± 0.13; HCD: 1.59 ± 0.13; LED + A: 0.85 ± 0.04; p = 0.0008). LED treatment improved anthropometric parameters, possibly associated with the histological alterations, thermogenesis and lipogenesis markers in white adipose tissue, and expression modulation in brown adipose tissue.


Assuntos
Dieta Hiperlipídica , Lipogênese , Masculino , Animais , Camundongos , Lipogênese/genética , Camundongos Obesos , Tecido Adiposo/metabolismo , Tecido Adiposo Marrom/metabolismo , Tecido Adiposo Marrom/patologia , Termogênese , Camundongos Endogâmicos C57BL
20.
Biomedicines ; 11(2)2023 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-36831092

RESUMO

Background: Resin composites undergo a certain degree of shrinkage when light-cured with different light sources available on the market, resulting in microleakage of dental restorations. The aim of the present study was to assess microleakage of class II restorations with bulk-fill resin composites cured with LED (light-emitting diode) and QTH (quartz tungsten-halogen light) units, both in cervical and occlusal areas of cavity preparations. Materials and Methods: In the present in vitro experimental study, a total of 30 human molar teeth were used, in which 60 class II cavities were prepared (mesial and distal) and restored with Filtek bulk fill resin composite. Restorations were equally distributed in 3 groups according to type of curing light: A, QTH (Litex 680A Dentamerica®); B, LED (Bluephase N® 3rd generation); and C, LED (Valo® 3rd generation). Each group was further subdivided into subgroups 1 and 2 according to IV-A or IV-B resin composite color. Restored teeth were subjected to 20,000 thermal cycles between 5° and 55 °C, then immersed in 1M silver nitrate solution for 24 h. Subsequently, the teeth were sectioned mesiodistally to obtain samples for observation under stereomicroscope in order to determine microleakage degree. Kruskal-Wallis H and Mann-Whitney U statistical tests were applied with a significance level of 5% (p < 0.05). Results: No statistically significant differences were found in the degree of microleakage of bulk-fill resin composites light-cured with LED and QTH units for both occlusal (p > 0.05) and cervical areas (p > 0.05). Additionally, no significant differences were found when comparing microleakage between occlusal and cervical areas (p > 0.05), regardless of lamp type. In addition, significant differences in microleakage degree were found between bulk-fill resins with IV-A and IV-B shades when they were light-cured with QTH at cervical level (p = 0.023). However, there were no significant differences when comparing these bulk-fill resin composite shades at occlusal level with LED (p > 0.05) and QTH (p > 0.05) units. Conclusions: Class II restorations with bulk-fill resin composite in IV-A and IV-B shades light-cured with third generation LED lamp and QTH showed no significant differences in microleakage when compared in both occlusal and cervical areas. On the other hand, significantly more microleakage was found at the cervical level when a darker shade of resin composite was used and light-cured with the QTH unit.

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