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1.
Emerg Infect Dis ; 30(12)2024 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-39374598

RESUMO

A febrile man in Italy who had traveled to Cuba in July 2024 was diagnosed with Oropouche fever. Reverse transcription PCR detected prolonged shedding of Oropouche virus RNA in whole blood, serum, urine, and semen. Sixteen days after symptom onset, replication-competent virus was detected in semen, suggesting risk for sexual transmission.

2.
Mem. Inst. Oswaldo Cruz ; 104(7): 992-997, Nov. 2009. ilus, tab
Artigo em Inglês | LILACS | ID: lil-534164

RESUMO

The positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin punctures of the ulcerated lesions and lymph nodes for in vitro cultures, which were processed under field conditions at the local health centre. Skin lesion biopsies were tested at a reference laboratory using kinetoplastid DNA (kDNA)-PCR to detect DNA. The median time required to obtain a positive culture from the skin samples was seven days and the contamination rate of the samples was 1.8 percent. The positivities of the cultures from skin lesions, kDNA-PCR and the combination of the two methods were 78.2 percent (95 percent CI: 73-82.6 percent), 89.3 percent (95 percent CI: 85.1-92.4 percent) and 97.1 percent (95 percent CI: 94.5-98.5 percent). We conclude that parasite culture is a feasible method for the detection of Leishmania in field conditions and that the combination of culture and PCR has a potential role for the diagnosis of CL in candidates for clinical trials.


Assuntos
Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Técnicas de Cultura de Células/métodos , DNA de Cinetoplasto/genética , Leishmania braziliensis , Leishmaniose Cutânea/diagnóstico , Reação em Cadeia da Polimerase/métodos , Brasil , Distribuição de Qui-Quadrado , Leishmania braziliensis/genética , Leishmania braziliensis/isolamento & purificação , Linfonodos/parasitologia , Análise de Sequência de DNA , Úlcera Cutânea/parasitologia , Adulto Jovem
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