RESUMO
Background: Ovarian enlargement in mares can either be a physiological or pathological condition associated with neoplastic or non-neoplastic causes. Neoplasia is more prominent in elderly than young horses. Granulosa cell tumors are the most common neoplastic cause of ovarian enlargement, and thecoma is the rarest. Thecoma is a benign ovarian stromal neoplasia originating from the sexual cords of the embryonic gonad and can affect the production of steroid hormones, leading to changes in behavior and the estrous cycle. If only one ovary is affected, after unilateral ovariectomy, usually the mare can still be used for reproductive purposes. Herein, we report a case of ovarian thecoma in an elderly Crioulo mare. Case: A 15-year-old Crioulo mare was evaluated due to progressive weight loss. The mare presented a low body score, and on physical examination, all parameters were within reference limits. Hematological examination revealed anemia, hyperfibrinogenemia, and neutrophilia. Serum biochemistry examination were within the physiological limits. Rectal palpation revealed an increase in volume in the left ovary (4.3 × 2.9 × 3 cm), smooth consistency and no associated pain in either of the ovaries. Rectal ultrasonography revealed a heterogeneous pattern, suggesting a neoplasm. As a treatment modality and for definitive diagnosis, the mare underwent a unilateral flank ovariectomy laparoscopically in the quadrupedal position. On histopathological evaluation of the left ovary, the sample showed cysts associated with proliferating spindle cells arranged in a solid arrangement, occasionally forming eddies, in addition to hemosiderosis, with clear cytoplasm and lipid vacuoles. Macroscopically, the ovary presented with a regular surface, smooth to firm consistency, a homogeneous brownish appearance with blackened foci, and brownish fluid-filled cysts. These findings confirmed thecoma of the left ovary in the mare. Discussion: Ovarian enlargement in mares can be associated to the presence of neoplasia. Clinical signs of ovarian neoplasms can be nonspecific; therefore, to achieve a definitive diagnosis, it is imperative to evaluate and compare the patient's history, physical examination, and results of complementary examinations. Excessive production of hormones lead to behavioral changes in mares with ovarian tumors, such as masculinized or aggressive behavior, and anestrus persistence. Thecomas may or may not interfere with steroid production and levels. On ultrasound examination, ovarian neoplasms may present similar echogenicity patterns. Histopathological evaluation is the best diagnostic approach after an ovariectomy. The prognosis of ovariectomy is considerably successful with the mare regaining reproductive health, although some mares may become infertile, with a small, inactive contralateral ovary. Thecoma is a rare neoplasm in horses; however, owing to its potential to cause altered reproductive functions, it should be considered as a differential diagnosis in mares with increased ovarian volume.
Assuntos
Animais , Feminino , Tumor da Célula Tecal/patologia , Células Tecais/citologia , CavalosRESUMO
BACKGROUND: Chronic lung diseases are characterized by airway inflammation and remodelling of the lung parenchyma that triggers considerable impairment of respiratory function. OBJECTIVE: In this study, two compounds belonging to the N-acylhydrazone class were evaluated, aiming to identify new therapeutic agents for pulmonary inflammatory diseases. MATERIALS AND METHODS: The acute toxicity of 2-cyano-N'-(3-ethoxy-4-hydroxybenzylidene)- acetohydrazide (JR-12) and N'-benzylidene-2-cyano-3-phenylacrylohydrazide (JR09-Bz) was evaluated. Afterwards, they were tested in models of ovalbumin (OVA)-induced allergic asthma and pleurisy, bleomycin-induced pulmonary fibrosis, in addition to mucolytic activity. RESULTS AND DISCUSSION: The compounds did not show toxicity at the dose of 2,000 mg/kg, and no animal died. On OVA-induced pleurisy, animals treated with JR-12 or JR09-Bz at a dose of 10 mg/kg (orally) showed significant inhibition of the leukocyte infiltrate in the bronchoalveolar lavage by 62.5% and 61.5%, respectively, compared to the control group. The compounds JR-12 and JR09-Bz were also active in blocking the allergic asthmatic response triggered by OVA, reducing the leukocyte infiltrate by 73.1% and 69.8%, respectively. Histopathological changes and mast cell migration in treated animals with JR-12 or JR09-Bz were similar to treatment with the reference drugs dexamethasone and montelukast. JR-12 and JR09-Bz also reversed airway remodeling in animals on the bleomycin-induced fibrosis model compared to the control group. Furthermore, it was observed that N-arylhydrazone derivatives showed expectorant and mucolytic activities, increasing mucus secretion by 45.6% and 63.8% for JR-12 and JR09-Bz, respectively. CONCLUSION: Together, the results show that JR-12 and JR09-Bz showed promising activity against airway inflammation, as well as low toxicity.
Assuntos
Asma , Pleurisia , Pneumonia , Animais , Camundongos , Ovalbumina/efeitos adversos , Expectorantes/efeitos adversos , Líquido da Lavagem Broncoalveolar , Modelos Animais de Doenças , Asma/tratamento farmacológico , Asma/patologia , Pneumonia/induzido quimicamente , Pneumonia/tratamento farmacológico , Pulmão/patologia , Alérgenos , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Dexametasona , Pleurisia/tratamento farmacológico , Pleurisia/patologia , Bleomicina/efeitos adversos , Camundongos Endogâmicos BALB C , CitocinasRESUMO
The objective of this study was to evaluate the acute toxicity of formalin and its level of therapeutic safety in early stages of Lophiosilurus alexandri. Experiment 1, larvae 7 days after hatching (DAH) were exposed to 43.2, 86.4, 172.8, 345.6, 691.2, 1404.0â¯mg/L of formalin. Experiment 2, juveniles with 22 DAH exposed to 54, 108, 216, 432, 648â¯mg/L. Experiment 3, 45 DAH exposed to 86.4, 172.8, 345.6, 691.2, 1036.8â¯mg/L. The experiments had a control without addition of formalin and all were carried out in duplicate. The LC50-12â¯h were: Experiment 1â¯=â¯108.86â¯mg/L; 2: 152.74â¯mg/L; 3: 244.38â¯mg/L of formalin. The respective safety levels were: Experiment 1â¯=â¯66.22â¯mg/L (1â¯h), 10.89â¯mg/L (12â¯h); 2â¯=â¯49.17â¯mg/L (2â¯h), 15.27â¯mg/L (12â¯h); 3â¯=â¯68.89â¯mg/L (2â¯h), 24.44â¯mg/L (12â¯h). The results showed that the developmental stage influenced the sensitivity of animals to formalin.
Assuntos
Peixes-Gato/fisiologia , Formaldeído/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Desinfetantes/toxicidade , Água Doce , Larva , Dose Letal MedianaRESUMO
Objetivo: Analisar biópsias de pele de pacientes com suspeita de hanseníase e LTA atendidos no serviço de dermatologia de um hospital público de Recife-PE. Métodos: Trata-se de um estudo de caráter retrospectivo, transversal, descritivo, qualitativo no qual foram analisadas biópsias de pele de pacientes com suspeita de hanseníase e/ou LTA, atendidos no serviço de dermatologia do Hospital das Clínicas, Recife-PE, no período de janeiro de 2016 a abril de 2018. Resultados: Um total de 46 pacientes com suspeita de hanseníase e/ou LTA foi atendido, as idades variaram entre 13 a 90 anos, 22 pacientes eram do sexo feminino e 24 do sexo masculino. Dentre esses 46 pacientes, havia 25 (54%) com suspeita de leishmaniose, sendo 10 (40%) confirmados com positividade para Leishmania braziliensis pela PCR, e 12 (26%) confirmados para hanseníase. Conclusão: A análise histopatológica contribuiu para confirmação e diagnóstico diferencial entre hanseníase e leishmaniose tegumentar, além de auxiliar na diferenciação com outras doenças de características clínicas semelhantes.
Objective: To analyze the skin biopsies of patients with suspicion of leprosy and LTA treated at the dermatology department of a public hospital in Recife-PE. Methods: This was a cross-sectional, descriptive and qualitative retrospective study of skin biopsies of patients with suspected leprosy and/or ACL, treated at the dermatology department of the Hospital das Clínicas, Recife, period from January 2016 to April 2018. Results: A total of 46 patients with suspected leprosy and/or LTA were attended, ages ranged from 13 to 90 years, 22 were female and 24 were male. Among these 46 patients, 25 (54%) with suspected leishmaniasis, with 10 (40%) confirmed positive for Leishmania braziliensis by PCR, and 12 (26%) confirmed for leprosy. Conclusion: The histopathological analysis contributed for confirmation and differential diagnosis of leprosis and cutaneous leishmaniasis, moreover it served to help in the differentiation of other diseases with similar clinical characteristics
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Biópsia , Leishmaniose Cutânea/diagnóstico , Diagnóstico Diferencial , Hanseníase/diagnósticoRESUMO
O carcinoma de glândulas hepatoides é uma neoplasia maligna, de origem epitelial, que acomete cães machos inteiros e com idade avançada. O presente trabalho relata o caso de dois caninos machos, o primeiro da raça Teckel e o segundo sem raça definida, 13 anos e 15 anos de idade respectivamente, o segundo apresentando sintomas de hematúria e piúria, com histórico de ectoparasitismo e que apresentavam nódulo em região de prepúcio. Foram realizados exames pré-operatórios e de pesquisa de metástase os quais não indicaram nada digno de nota. Houve o direcionamento para a realização de excisão cirúrgica dos nódulos os quais foram encaminhados para histopatologia e tiveram como diagnóstico morfológico o carcinoma de glândulas hepatoides.
Hepatoid gland carcinoma is a malignant neoplasm of epithelial origin that affects whole male dogs with advanced age. The present study reports the case of two male canines, the first of the Teckel breed and the second of no defined breed, 13 years and 15 years of age respectively, the second presenting symptoms of hematuria and pyuria, with a history of ectoparasitism and who presented with a nodule in foreskin region. Preoperative and metastatic research examinations were performed which indicated nothing of note. The patients were referred for surgical excision of the nodules, which were referred for histopathology and their morphological diagnosis was carcinoma of the hepatoid glands.
Assuntos
Animais , Cães , Piúria/veterinária , Carcinoma/veterinária , Doenças do Cão , Prepúcio do Pênis/anormalidadesRESUMO
Objetivo: evaluar las causas de muerte fetal, mediante antecedentes clínicos maternos,análisis de los hallazgos de la autopsia fetal y estudio de la placenta. Materiales y Métodos: se realizó un análisis retrospectivo de 332 muertes fetales durante el periodo comprendido de 2005-2009 ocurridas en el Hospital Universitario de Santander (HUS)Bucaramanga, Colombia. Se incluyeron análisis histopatológicos de la placenta y el feto. Se planteó la siguiente clasificación de las causas de muerte fetal 1) Hipoxia fetal intrínseca 2) Anomalías congénitas 3) Infecciones ascendentes 4) Traumatismos del parto 5) Hidrops fetal. Se determinaron como no clasificables a fetos macerados. Se establecieron subgrupos de análisis de la población como edad materna, edad fetal y se analizan los datos mediante porcentajes y promedios. Resultados: el promedio de edad materna fue de 25,9 años, el promedio de edad gestacional fue de 28,9 semanas. Se conoció el tipo de muerte en el 87,96% de los casos. Las causas más frecuentes de muerte fueron la hipoxia fetal intrínseca 46,67%: insuficiencia placentaria 17,5%, desprendimiento placentario 6,6%, infarto placentario 6,3%, HTA materna 4,5%. Anomalías congénitas 18,07%, infección bacteriana ascendente 16,26%, causa desconocida 12,04%. En gestaciones entre 20-29 semanas la principal causa de muerte fue patología placentaria (43.97%); en cuanto a 37-42 semanas la principal causa fue las anomalías congénitas (5,1%). Conclusiones: el presente estudio muestra que las principales causas de óbito fetal están vinculadas con patologías que pueden ser detectadas y manejadas de manera adecuada y expone la importancia de la autopsia fetal sumadoal estudio anatomo patológico de la placenta a la hora de determinar dichas causas.
Objective: to determine the causes of fetal death through maternal medical records review, analysis of the fetal autopsy findings and pathological examination of the placenta. Materials and Methods: a retrospective study of 322 fetal deaths was madeduring the period of 2005-2009 occurred in the Hospital Universitario de Santander(HUS), Bucaramanga, Colombia. Histopathological analysis of the fetus and placenta were included. The following classification was proposed in regards to the causes offetal death: 1. Intrisec fetal hypoxia 2. Congenital abnormalities 3. Ascending Infections4. Trauma during birth 5. Fetal hydrops. Macerated fetuses were considered not classified. The establishment of Subgroups analyzing population such as maternalage, gestational age. Results: a mean maternal age was 25,9 years and meangestational age: 28,9 weeks. The most frequent causes of death found where intrinsicfetal hypoxia 46.67%, placental insufficiency 17,5%, abruptio 6.6%, placental infarction6,3%, Maternal Hypertension 4,5%, congenital abnormalities 18,07%, ascendingbacterial infection 16,26%, unknown cause 12,04%. In pregnancies between 20-29weeks the main cause of death was the placental disease and in 37-42 weeks was congenital anomalies (5.1%). Data were analyzed using percentages and averages. Conclusion: the present study shows that the main causes of stillbirth are linked topathologies that can be detected and properly handled, and exposes the importanceof the fetal autopsy added to the anatomical pathology study of the placenta when determining these causes.
Assuntos
Causas de Morte , Morte Fetal , PatologiaRESUMO
El Angiomixoma agresivo es una neoplasia de diferenciación incierta descrita por primera vez por Steeper y Rosai (1983), que tiene predilección por la región pélvica y perineal de mujeres entre la 3ra y la 6ta década de la vida, afectando con menor frecuencia al sexo masculino, con casos descritos de localización escrotal. En Venezuela hasta la fecha, no hay casos informados de este tumor en pacientes masculinos. Presentamos un caso de localización pélvica, confirmado mediante estudios de inmuno-histoquímica en un varón de 27 años de edad con una recidiva luego de 3 años de la exéresis inicial.
Aggressive angiomyxoma is a neoplasm of uncerta in differentiation first described by Steeper and Rosai (1983), which has a predilection for the pelvic and perineal region of females between the 3rd and 6th decade of life, affecting males less frequently, with reported cases of scrotal location. In Venezuela, to date, no cases were reported in male patients. A case of pelvic location we presented, confirmed by immunohistochemistry studies in a male of 27 years-old with a relapse after three years of the initial excision.
Assuntos
Humanos , Masculino , Adulto , Mixoma/cirurgia , Mixoma/diagnóstico , Neoplasias Pélvicas/diagnóstico , Técnicas Histológicas/métodos , OncologiaRESUMO
A avaliação do curso da infecção por Toxoplasma gondii em camundongos alimentados com micotoxinas, utilizando a histopatologia e imuno-histoquímica, demonstrou a ocorrência de lesões mais acentuadas nos animais infectados e expostos às toxinas. Diferentes quadros de inflamação e necrose encefálica e hepática mostraram que a associação entre parasitismo e a ingestão das micotoxinas causou agravamento nas lesões. A fumonisina B1(FB1, isolada ou associada a outras micotoxinas) foi responsável por alterações celulares como gigantismo nuclear, hipertrofia celular e células atípicas em tecido hepático. A FB1 foi também relacionada com quadros de desmielinização em tecido cerebral. A presença de cistos ou formas livres da cepa ME-49 de T. gondii foi constatada tanto em animais controle quanto nos alimentados com micotoxinas, mostrando que a cepa do parasita, nesta linhagem C57BL/6 de camundongos, apresenta formas características de fase aguda e crônica da infecção.
The course of toxoplasmosis in mice fed with mycotoxins was evaluated by histopatology and immunohistochemistry. The most severe lesions was seen in animals infected by T. gondii and fed on mycotoxins, when they were compered with control animals. Encephalitis, hepatitis, necrosis foci in the brain and liver showed that the association parasite infection and micotoxicosis aggravated the lesions. The fumonisin B1(isolated or in adiccion to others mycotoxins) was responsable for cellular alteration like nuclear enlargement, cellular hypertrophy, and atypical cells in hepatic tissue. This mycotoxin was also related to brain desmyelinization. The presence of cysts or free parasites was observed in control and intoxicated animals. This result showed that ME49 strain of T. gondii, in C57BL/6 mice, can display typical forms of acute and chronic infection.
Assuntos
Cobaias , Camundongos/classificação , Imuno-Histoquímica/métodos , Patologia , Dieta , Doenças Parasitárias , Inflamação/complicações , Micotoxinas/efeitos adversos , Toxoplasma/patogenicidadeRESUMO
A avaliação do curso da infecção por Toxoplasma gondii em camundongos alimentados com micotoxinas, utilizando a histopatologia e imuno-histoquímica, demonstrou a ocorrência de lesões mais acentuadas nos animais infectados e expostos às toxinas. Diferentes quadros de inflamação e necrose encefálica e hepática mostraram que a associação entre parasitismo e a ingestão das micotoxinas causou agravamento nas lesões. A fumonisina B1(FB1, isolada ou associada a outras micotoxinas) foi responsável por alterações celulares como gigantismo nuclear, hipertrofia celular e células atípicas em tecido hepático. A FB1 foi também relacionada com quadros de desmielinização em tecido cerebral. A presença de cistos ou formas livres da cepa ME-49 de T. gondii foi constatada tanto em animais controle quanto nos alimentados com micotoxinas, mostrando que a cepa do parasita, nesta linhagem C57BL/6 de camundongos, apresenta formas características de fase aguda e crônica da infecção.(AU)
The course of toxoplasmosis in mice fed with mycotoxins was evaluated by histopatology and immunohistochemistry. The most severe lesions was seen in animals infected by T. gondii and fed on mycotoxins, when they were compered with control animals. Encephalitis, hepatitis, necrosis foci in the brain and liver showed that the association parasite infection and micotoxicosis aggravated the lesions. The fumonisin B1(isolated or in adiccion to others mycotoxins) was responsable for cellular alteration like nuclear enlargement, cellular hypertrophy, and atypical cells in hepatic tissue. This mycotoxin was also related to brain desmyelinization. The presence of cysts or free parasites was observed in control and intoxicated animals. This result showed that ME49 strain of T. gondii, in C57BL/6 mice, can display typical forms of acute and chronic infection.(AU)
Assuntos
Cobaias , Patologia , Imuno-Histoquímica/métodos , Camundongos/classificação , Toxoplasma/patogenicidade , Dieta , Micotoxinas/efeitos adversos , Inflamação/complicações , Doenças ParasitáriasRESUMO
OBJETIVE: An experimental study was done to evaluate the effect of nicotine on the healing of abdominal wall. METHODS: Sexteen Wistar rats weighing 210± 8g were randomly separated into two groups of eight rats each. In the group A Nicotine (Nicotinel Ò) was implanted in the back subcutaneous 5mg/Kg each two days, begining at the fifth preoperative day. A 5cm median laparotomy was done, sutured with nylon 5-0 and the rats were observed in individual cages. The group B (control) didnt use nicotine. A multiperfurated silicone tube was implanted subcutaneously near the abdominal sutured lesion. In the tenth postoperative day 1ml of serous liquid was harvested from the silicone tube and a pO2 dosage was done. The rats were killed with overdose of anesthetic. A 2cm width transversal segment of abdominal wall was submited to tensile strength test by a tensiometer. A biopsy of the sutured area including muscle, aporeurosis and peritoneum was processed for histopatologic analysis by a digitalised system. The data were processed by the ANOVA and Newman-Keuls tests, considering significant the differences when p 0,05. RESULTS: In the group A the pO2 on the tissue serous liquid reached 17,75± 3,4 mmHg and in the group B (control) the pO2 = 40,75± 6,4 mmHg (p 0,01). The tensile strength reached 728,5± 161,75gf in the group A and 1241,6± 232gf in the control one (p 0,01).The mean density of the histopatologic elements was 105± 17,1 in group A and 146,2± 8,8 in group B (p 0,01). CONCLUSION: after evaluation of tissue pO2 , tensiometry and histopatology, the data permited to conclude that the nicotine has a deleterious effect on the healing the abdominal wall of rats.
OBJETIVO: Avaliar o efeito da nicotina na cicatrização da camada musculoaponeurótica da parede abdominal. MÉTODOS: Estudo experimental em que foram usados 16 ratos da raça Wistar pesando em média 210± 8g, separados aleatoriamente em 2 grupos de 8. Nos animais do grupo A foi implantado disco de nicotina (Nicotinel Ò) na dose de 5mg/Kg de peso/dia no subcutâneo da região dorsal, trocado a cada dois dias, a partir do 5º dia antes da operação em que foi feita laparotomia mediana de 5 cm, até o 10º dia de observação. No grupo B (controle) foram usados discos de celulose com o mesmo diâmetro. Tubo de silicone multiperfurado foi implantado no subcutâneo a 1cm da lesão da parede abdominal. A camada musculoaponeurótica e a pele foram suturadas com fio de nylon 5-0. No 10º dia pós-operatório foi colhido 1ml de líquido seroso do tubo de silicone por punção percutânea para dosagem de pO2 e os animais receberam dose letal de anestésico. Foi ressecado um segmento da camada musculoaponeurótica com 2cm de largura para tensiometria, em seguida processado e corado em HE e tricrômico de Masson para análise quantitativa dos dados histopatológicos em sistema digitalizado. A análise estatística foi feita pelo ANOVA e teste Newman-Keuls, com significância 0,05. RESULTADOS: No grupo A a pO2 do líquido tecidual atingiu o valor 17,75± 3,4 mmHg e no grupo B (controle) a pO2 = 40,75± 6,4 mmHg (p 0,01). A resistência à tensão apresentou o valor de 728,5± 161,75gf no grupo A e 1241,6± 232gf no grupo de controle (p 0,01), coincidindo com os achados da pO2. A densidade média dos elementos histopatológicos estudados foi de 105± 17,1 nos animais do grupo A e 146,2± 8,8 no grupo B (p 0,01). CONCLUSÃO: após avaliação da pO2 tecidual, tensiometria e histopatologia, concluiu-se que a nicotina por via subcutânea exerce efeito deletério sobre a cicatrização de lesões da parede abdominal de ratos.
RESUMO
OBJETIVE: An experimental study was done to evaluate the effect of nicotine on the healing of abdominal wall. METHODS: Sexteen Wistar rats weighing 210± 8g were randomly separated into two groups of eight rats each. In the group A Nicotine (Nicotinel Ò) was implanted in the back subcutaneous 5mg/Kg each two days, begining at the fifth preoperative day. A 5cm median laparotomy was done, sutured with nylon 5-0 and the rats were observed in individual cages. The group B (control) didnt use nicotine. A multiperfurated silicone tube was implanted subcutaneously near the abdominal sutured lesion. In the tenth postoperative day 1ml of serous liquid was harvested from the silicone tube and a pO2 dosage was done. The rats were killed with overdose of anesthetic. A 2cm width transversal segment of abdominal wall was submited to tensile strength test by a tensiometer. A biopsy of the sutured area including muscle, aporeurosis and peritoneum was processed for histopatologic analysis by a digitalised system. The data were processed by the ANOVA and Newman-Keuls tests, considering significant the differences when p 0,05. RESULTS: In the group A the pO2 on the tissue serous liquid reached 17,75± 3,4 mmHg and in the group B (control) the pO2 = 40,75± 6,4 mmHg (p 0,01). The tensile strength reached 728,5± 161,75gf in the group A and 1241,6± 232gf in the control one (p 0,01).The mean density of the histopatologic elements was 105± 17,1 in group A and 146,2± 8,8 in group B (p 0,01). CONCLUSION: after evaluation of tissue pO2 , tensiometry and histopatology, the data permited to conclude that the nicotine has a deleterious effect on the healing the abdominal wall of rats.
OBJETIVO: Avaliar o efeito da nicotina na cicatrização da camada musculoaponeurótica da parede abdominal. MÉTODOS: Estudo experimental em que foram usados 16 ratos da raça Wistar pesando em média 210± 8g, separados aleatoriamente em 2 grupos de 8. Nos animais do grupo A foi implantado disco de nicotina (Nicotinel Ò) na dose de 5mg/Kg de peso/dia no subcutâneo da região dorsal, trocado a cada dois dias, a partir do 5º dia antes da operação em que foi feita laparotomia mediana de 5 cm, até o 10º dia de observação. No grupo B (controle) foram usados discos de celulose com o mesmo diâmetro. Tubo de silicone multiperfurado foi implantado no subcutâneo a 1cm da lesão da parede abdominal. A camada musculoaponeurótica e a pele foram suturadas com fio de nylon 5-0. No 10º dia pós-operatório foi colhido 1ml de líquido seroso do tubo de silicone por punção percutânea para dosagem de pO2 e os animais receberam dose letal de anestésico. Foi ressecado um segmento da camada musculoaponeurótica com 2cm de largura para tensiometria, em seguida processado e corado em HE e tricrômico de Masson para análise quantitativa dos dados histopatológicos em sistema digitalizado. A análise estatística foi feita pelo ANOVA e teste Newman-Keuls, com significância 0,05. RESULTADOS: No grupo A a pO2 do líquido tecidual atingiu o valor 17,75± 3,4 mmHg e no grupo B (controle) a pO2 = 40,75± 6,4 mmHg (p 0,01). A resistência à tensão apresentou o valor de 728,5± 161,75gf no grupo A e 1241,6± 232gf no grupo de controle (p 0,01), coincidindo com os achados da pO2. A densidade média dos elementos histopatológicos estudados foi de 105± 17,1 nos animais do grupo A e 146,2± 8,8 no grupo B (p 0,01). CONCLUSÃO: após avaliação da pO2 tecidual, tensiometria e histopatologia, concluiu-se que a nicotina por via subcutânea exerce efeito deletério sobre a cicatrização de lesões da parede abdominal de ratos.
RESUMO
Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.
Seis suínos mestiços Landrace-Large White, com aproximadamente 120 dias de idade, foram inoculados, via endovenosa, com taquizoitos das cepas AS 28, LIV-IV e N de Toxoplasma gondii e analisados quanto aos aspectos clínico, sorológico, parasitológico e histopatológico. Para a avaliação das técnicas de diagnóstico post-mortem, os animais foram sacrificados e necropsiados nas fases aguda (n=3) e crônica (n=3) da infecção. Tecidos formolizados e parafinados foram submetidos às técnicas de Hematoxilina-Eosina (H.E.), Imunofluorescência Indireta (RIFI) e Peroxidase Anti-Peroxidase (PAP). A H.E. revelou-se eficiente na análise das alterações morfológicas dos diferentes tecidos, mas não se mostrou útil para detectar T. gondii. A RIFI e a PAP revelaram o parasito na intimidade dos tecidos, sendo que a última mostrou-se mais eficiente, uma vez que, além de demonstrar parasitas, permitiu a análise das alterações morfológicas dos órgãos estudados.
RESUMO
Six 120 days old Landrace/Large-white swines with were inoculated with taquizoites of the AS 28, LIV-IV and N Toxoplasma gondii strains and analysed under clinical, sorological, parasitological and histopathological aspects. In order to evaluate some postmortem diagnostic techniques, three swines were sacrificed in the acute phase and three swines in the chronic phase of the infection. Formalin-fixed and parafin-embedded tissues were submitted to Eosin-Hematoxilin, Indirect Immunofluorescence (IFA) and Peroxidase Anti-Peroxidase (PAP) techniques. The Eosin-Hematoxilin showed to be efficient in the analysis of the morphological alterations in the tissues, but did not detect any form of the parasite. Both, IFA and PAP revealed taquizoites of T. gondii, although the last one showed to be more effective because it also allowed an analysis of the morphological tissue alterations.
Seis suínos mestiços Landrace-Large White, com aproximadamente 120 dias de idade, foram inoculados, via endovenosa, com taquizoitos das cepas AS 28, LIV-IV e N de Toxoplasma gondii e analisados quanto aos aspectos clínico, sorológico, parasitológico e histopatológico. Para a avaliação das técnicas de diagnóstico post-mortem, os animais foram sacrificados e necropsiados nas fases aguda (n=3) e crônica (n=3) da infecção. Tecidos formolizados e parafinados foram submetidos às técnicas de Hematoxilina-Eosina (H.E.), Imunofluorescência Indireta (RIFI) e Peroxidase Anti-Peroxidase (PAP). A H.E. revelou-se eficiente na análise das alterações morfológicas dos diferentes tecidos, mas não se mostrou útil para detectar T. gondii. A RIFI e a PAP revelaram o parasito na intimidade dos tecidos, sendo que a última mostrou-se mais eficiente, uma vez que, além de demonstrar parasitas, permitiu a análise das alterações morfológicas dos órgãos estudados.