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O objetivo do presente estudo foi de comparar o diâmetro dos cones acessórios FM EL calibrados por duas réguas calibradoras com seus respectivos diâmetros nominais. Foram calibrados 80 cones de guta percha FM EL (Odous de Deus) utilizando duas réguas calibradoras das seguintes marcas: Prisma e Denco. Os cones foram divididos em 8 grupos (n=10) de acordo com a régua e com o diâmetro em que foram calibrados. Posteriormente, os cones foram fotografados e tiveram seus D0 mensurados através do software ImageJ. Após a realização da análise estatística utilizando-se os testes de Normalidade de Shapiro-Wilk e o teste t Student (Distribuição Normal), obteve-se os seguintes resultados: não houve diferença estatisticamente significativa com o valor de referência na régua prisma nos cones 40, isto é, em todas as outras situações encontrou-se diferença com os valores de referência. Quando foram comparados os valores das medianas e desvio padrão das duas réguas calibradoras também houve diferença estatisticamente significativa (p<0,05) nos cones 25, 35 e 40. Os cones acessórios FM EL (Odous de Deus) calibrados com a régua Denco diferiram dos diâmetros nominais da régua. Assim, deve-se estar atento à exatidão e precisão desses instrumentos a fim de se evitar possíveis erros de mensuração e interpretação capazes de comprometer o êxito da obturação no tratamento endodôntico.

The aim of the present study was to compare the diameter of the FM EL accessory cones calibrated by two calibrating rulers with their respective nominal diameters 80 FM EL gutta percha cones (Odous of God) were calibrated using two calibrating culers of the following brands: Prisma and Denco. The cones were divided in 8 groups (n=10) according to the ruler and the diameter in which they were calibrated. Posteriorly, the cones were photographed and nad their D0 neasured through the software ImageJ. After performing the statistical analysis using the Shapiro- Wilk Normality tests and the Student T test (Normal Distribution), the following results were obtained: there was no stastistically significant difference with the reference value in the prism rule in the cones 40, that is, in all other situations, a difference was found with the reference values. When the median values and standard deviation of the two calibrating rulers were compared, there was also a statistically significant difference (p<0,05) in cones 25, 35 and 40. The FM EL (Odous of God) accessory cones calibrated eita the Denco ruler differed from the nominal diameters of the ruler. Thus, one must pay attention to the accuracy and precision of these instruments in order to avoid possible errors of measurement and interpretation capable of compromising the success of filling in endodontic treatment.

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Aim: The tensile strength and modulus of elasticity of gutta-percha cones can be chemically altered due to disinfectant solutions. Therefore, the aim of the present study was to compare tensile strength and elastic modulus of gutta-percha cones subjected to sodium hypochlorite (NaOCl) disinfection at different times. Materials and Methods: This in vitro and longitudinal experimental study consisted of 45 gutta-percha cones, divided equally into three groups: Group 1 (disinfection with 2.5% NaOCl), Group 2 (disinfection with 5.25% NaOCl), and control group. All groups were subdivided according to immersion times for 1, 5, and 10 minutes. Tensile strength and elastic modulus were measured with a universal testing machine. For comparing more than two independent groups, parametric analysis of variance test with Sheffe's post hoc was used and for multivariate analysis, and multivariate analysis of variance test based on Pillai's Trace was used. In all statistical analysis, a significance level P ≤ 0.05 was considered. Results: When comparing the tensile strength of gutta-percha cones, no significant differences were observed after being immersed at 1, 5, and 10 minutes in NaOCl 2.5% (P = 0.715) and 5.25% (P = 0.585). Regarding the elastic modulus, a significant decrease (P < 0.05) was observed in those that were immersed in NaOCl 2.5% and 5.25% for 1, 5, and 10 minutes. Furthermore, increased NaOCl concentration significantly reduced the elastic modulus (P < 0.001). However, there were no significant differences in tensile strength (P > 0.05) and elastic modulus (P > 0.05), when evaluating the interaction between NaOCl concentration and time. Conclusion: Increasing NaOCl concentration significantly reduced the modulus of elasticity without affecting the tensile strength of gutta-percha cones, regardless of immersion time. Furthermore, the interaction of time and NaOCl concentration did not significantly affect the tensile strength and elastic modulus.

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To evaluate the impact on the quality of filling with of low-fusion and conventional gutta-percha cones. Thirty-six maxillary canines were prepared and divided into three groups: I-conventional cone with Downpack at 200 °C at 4 mm from the WL; II-low-fusion cone with Downpack at 100 °C up to 4 mm from the WL; III-low-fusion cone with Downpack at 100 °C up to 7 mm from the WL. Temperature variations were measured in thirds on the external surface of the root. The bond strength was evaluated using the push-out test. The adhesive interface was analyzed by scanning electron microscopy. The bond strength and the temperature variation data were analyzed using analysis of variance and the failure type using the chi-square test. The low-fusion cone group with 7 mm Downpack showed higher bond strength (4.2 ± 2.7) compared with conventional cones (2.8 ± 1.6) and low-fusion cones with 4 mm Downpack (2.9 ± 1.6) (p < 0.05), with occurrence of a higher number of adhesive failures to the filling material and mixed failures. Relative to temperature variation, there was less temperature change in the apical third, in the low-fusion cone with Downpack 7 mm (1.0 ± 1.0) (p < 0.05). The use of low-fusion cones allowed the continuous wave condensation technique to be performed at a lower depth of Downpack at 100 °C at 7 mm, with less heating in the apical third, without compromising the quality of filling. Using gutta-percha cones with low fusion, which permits a lower condensation temperature and reduced Downpack depth, maintains the quality of filling, in order to minimize possible damage to the periapical tissues.

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This study aimed to compare the efficacy of 2.5% sodium hypochlorite (NaOCl), 2.5% calcium hypochlorite [Ca(OCl)2], and 2% chlorhexidine (CHX) in the rapid disinfection of gutta-percha cones contaminated with Candida albicans. The minimum inhibitory and minimum fungicidal concentrations of each solution for C. albicans were determined and the ability of each solution to destroy and inhibit biofilm in culture wells was tested. In addition, ninety-eight gutta-percha cones contaminated with the fungal suspension were disinfected according to the type of solution (2.5% NaOCl, 2.5% Ca(OCl)2 or 2% CHX) in its different application methods (without agitation, ultrasonic agitation or agitation with Easy Clean), and regarding the exposure time to each irrigating solution (1 or 5 min). Next, the samples were checked for turbidity and evaluation of viable colonies. The compounds that showed the best performance in biofilm destruction were NaOCl and Ca(OCl)2 at a concentration of 2xMIC (p < 0.001). Regarding inhibited biofilm, the only compound that was effective at all MIC concentrations tested was 2.5% Ca(OCl)2 (p < 0.0001). Regarding the viable colonies, all solutions were effective concerning the control group, for all application methods, in 1 and 5 min (p < 0.05). The densitometer reading showed that CHX was the only effective solution in all application methods performed (p < 0.05). The results demonstrate that all tested solutions were effective in the rapid decontamination of cones contaminated with C. albicans.

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Abstract This study aimed to compare the quality of root canal obturation (ratio of area occupied by gutta-percha (G), sealer (S), and presence of voids (V)) in different anatomical irregularities (intercanal communications, lateral irregularities, and accessory canals) located at different thirds of the root canal system of mandibular molar replicas. Sixty-seven 3D printed replicas of an accessed mandibular molar were prepared using ProGlider and ProTaper Gold rotatory systems. Three specimens were randomly selected to be used as controls and did not receive further treatment. The rest were randomly distributed in 4 experimental groups to be obturated using either cold lateral compaction (LC), continuous wave of condensation (CW), and core-carrier obturation (ThermafilPlus (TH) or GuttaCore (GC)) (n=16 per group). AHPlus® sealer was used in all groups. The three controls and a specimen from each experimental group were scanned using micro-computed tomography. The rest of the replicas were sectioned at the sites of anatomical irregularities and examined at 30× magnification. The G, S, and V ratios were calculated dividing the area occupied with each element by the total root canal area and then compared among groups using the Kruskal-Wallis test. Voids were present in all obturation techniques with ratios from 0.01 to 0.15. CW obtained a significantly higher G ratio in the irregularity located in the coronal third (0.882) than LC (0.681), TH (0.773), and GC (0.801) (p<0.05). TH and GC achieved significantly higher G ratios in those located in the apical third (p<0.05). The worst quality of obturation was observed in the loop accessory canal with all obturation techniques. Whitin the limitations of this study, it can be concluded that CW and core-carrier obturation are respectively the most effective techniques for obturating anatomical irregularities located in the coronal and the apical third.

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Introduction: This study aimed to evaluate if the apical patency can influence the adaptation of the master cone of gutta-percha, quantifying the volume of voids and areas at the last 2 mm of the working length (WL). Materials and Methods: Sixty distobuccal canals of extracted upper molars were divided into 3 groups (n=20) based on the patency length (A: passed 1 mm beyond the apical foramen (AF), B: at the AF and C: 1 mm short of the AF) with the Easy ProDesign Logic 25/0.01 file. Each group was subdivided into 2 subgroups (n=10) based on the WL established to prepare the root canal (1: 1 mm short of the AF or 2: at the AF) with the Easy ProDesign Logic 25/0.05 file. After the scan, void volumes were calculated from the last 2 mm of the WL and void areas at 0 mm, 1 mm and 2 mm of the last of WL, as well as the relation between the tip and taper of the master cone with the amount of void volume and areas. To investigate statistically significant differences, we used the Kruskal-Wallis statistical test. Results: There were more voids in volume when patency was achieved 1 mm beyond the AF and the root canal preparation was conducted at 1mm short of the AF (A1 group). Furthermore, the same group showed more voids areas, mainly on the last millimeter of WL. Conclusion: Achieving apical patency at 1 mm beyond the AF followed by instrumentation 1 mm short of the AF created more voids between the master gutta-percha cone and the root canal wall, especially on the last millimeter of WL.

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Objectives: This study was conducted to evaluate and compare the tip and taper compatibility of accessory gutta-percha points (AGPs) with various rotary and reciprocating instruments. Materials and Methods: Using a profile analyzer, tip and taper measurements were taken of 10 AGPs of each of the 14 models available from Odous de Deus and the 4 models available from Dentsply-Maillefer. Diameter measurements were taken at 1-mm intervals, from 3 mm from the tip (D3) to 16 mm. Results: Based on the mean values obtained, 3-dimensional (3D) models of the AGPs were drawn in Autodesk Fusion 360 and superimposed on 3D models of each instrument selected (Mtwo, Reciproc, RaCe, K3, and ProDesign Logic) to determine the compatibility between the instrument and the AGP. Data corresponding to the tips and tapers of the various AGPs, as well as the tip and taper differences between the AGPs and the instruments, were analyzed using descriptive statistics. The tapers of the AGPs were subject to the American National Standards Institute/American Dental Association No. 57 standard. The Odous de Deus extra-long medium and extra-long extra-medium AGPs were shown to be compatible with Mtwo, K3, and ProDesign Logic instruments with taper 0.06 and tip sizes 25 and 30, while the Dentsply fine and fine medium cones were compatible with Mtwo, RaCe, and K3 instruments with conicity of 0.04 and tip sizes 35 and 40. Conclusions: Both the Odous de Deus and Dentsply commercial brands included 2 AGP models with tip (D3) and taper compatibility with Mtwo, RaCe, K3, and/or Prodesign Logic instruments.

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A Odontologia Legal é a especialidade responsável por esclarecer demandas administrativas ou judiciais provenientes de diferentes esferas. Na esfera criminal, o processo de identificação humana engloba um conjunto de procedimentos que visam individualizar uma pessoa e, em diversas situações, a Odontologia Legal se destaca como um importante método de identificação humana. O objetivo desse trabalho foi apresentar um relato de caso em que a presença de um remanescente de guta-percha foi decisiva no processo de identificação humana. Após exame necroscópico em um cadáver e com a entrega do prontuário odontológico, foi realizada a comparação das informações recebidas do suspeito com aquelas coletadas no exame post mortem. Por meio dessa comparação, verificou-se que havia pontos coincidentes que permitiram que a identificação positiva da vítima fosse estabelecida, especialmente a presença, tanto no exame ante mortem quanto no post mortem, de um remanescente de guta-percha envolto por tecido cicatricial ósseo na região do dente 21. Os materiais dentários utilizados em procedimentos odontológicos, tais como o remanescente de guta-percha neste relato de caso pericial, são extremamente valiosos no processo de identificação humana

Forensic Dentistry is the specialty responsible for clarifying administrative or judicial demands from different spheres. In the criminal sphere, the human identification process encompasses a set of procedures that aim to individualize a person and, in several situations, and Forensic Dentistry stands out as an important method in human identification. The objective was to present a case report in which the presence of a gutta-percha remnant was important in the human identification process. After necroscopic examination and delivery of the dental records, the information received from the suspect was compared with that collected in the post mortem examination. Through this comparison, it was verified that there were coincident points that allowed the positive identification of the victim, especially the presence, both in the ante-mortem and in the post-mortem examination, of a gutta-percha remnant surrounded by bone scar tissue in the tooth region 21. Materials used in dental procedures, such as the gutta-percha remnant in this forensic case report, are extremely valuable in the human identification process

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The aim of this study was to evaluate the influence of surface topography of gutta-percha (GP) cones and plasticized disks of GP on the initial adhesion of Enterococcus faecalis (E. faecalis). The GP cones (Tanari and Dentsply brands) were cut 3 mm from the apical portion and fixed on a glass slide. To make the disks, the cones were thermoplasticized in standardized molds. The specimens were divided into groups according to the shape of the GP and the presence or absence of the bacteria. For contamination, the strain of E. faecalis (ATCC 29212) was used. The surface topography was analyzed using an atomic force microscope (AFM). The surface, roughness, and waviness parameters were evaluated by the Kruskal-Wallis and Dunn test. The comparison between disks and cones showed significant differences, where the cones were rougher, with a higher value attributed to the Dentsply cone (DC group). The same was observed for the waviness. After contamination, there was greater bacterial accumulation in cones, especially in their valleys, but both the surface and the topography became more homogeneous and smoother, with no differences between disks and cones of both brands. The topographic surface of the GP, at the micro and nanoscale, influences the initial adhesion of E. faecalis, with a greater tendency for contamination in regions associated with the presence of roughness and waviness. In this context, plasticization of GP is indicated, as it reduces surface irregularities compared to cones, contributing to less retention of bacteria.

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The aim of this study was to compare the capacity of two reciprocating NiTi instruments in removing gutta-percha/sealer material from simulated curved root canals (SCRC). The time required for filling material removal was also recorded. Twenty SCRCs were divided into two groups of 10 (n=10) samples each. In Group 1, the SCRC were prepared to a R25 Reciproc Blue instrument (RCPB; VDW, Munich, Germany). In Group 2 the SCRC were prepared to a Primary WaveOne Gold instrument (PWOG; Dentsply, Ballaigues, Switzerland). In both groups, the canals were filled with matched-taper single gutta-percha cones and AH Plus sealer. Filling materials were removed with R25 RCPB (Group 1) and PWOG (Group 2). The amount of remaining gutta-percha/sealer was calculated at three predetermined levels of evaluation located at 2, 6 and 10 mm from the WL and expressed in percentages. Canals re-treated with RCPB contained significantly less remaining gutta-percha/sealer compared tocanalspreparedwith PWOG (P=0.02). The RCPB instruments required significantly less time to complete the retreatment procedures (P<0.01). No unwinding or instrument separation was noted. RCPB instruments removed significantly more gutta-percha/sealer from simulated curved root canals than PWOG. However, neither of the tested instruments completely removed all filling materials.

El propósito del presente estudio fue comparar la capacidad de dos instrumentos de NiTi de movimiento reciproco para remover la obturación de gutapercha/sellador durante el retratamiento de conductos curvos simulados (SCRC). El tiempo requerido para la remoción del material fue también registrado. Se utilizaron veinte (n=20) SCRC divididos en dos grupos de diez especimenes (n=10) cada uno. En el Grupo 1 los SCRC se prepararon hasta un instrumento Reciproc Blue R25 (RCPB; VDW, Munich, Germany). En el Grupo 2, los SCRC se prepararon hasta un instrumento WaveOne Gold Primary (PWOG; Dentsply, Ballaigues, Switzerland). En ambos grupos los conductos se obturaron con cono único de gutapercha de conicidad creciente y el sellador AH Plus. La remoción de los materiales de obturación se realizó mediante los instrumentos RCPB R25 (Grupo 1) o PWOG (Grupo 2). La cantidad de gutapercha/sellador remanente se calculó en tres niveles de evaluaciónpredeterminadosubicados a 2, 6 y 10mm de la LT, y finalmente fue expresada en porcentajes. La cantidad de gutapercha/ sellador remanente en los SCRC retratados con RCPB fue significativamente menor en comparación con los que fueron retratados con PWOG (P=0.02). Los instrumentos RCPB requirieron un tiempo significativamente menor para completar el retratamiento (P<0.01). No se observaron deformaciones o separación de los instrumentos. Los instrumentos RCPB removieron una cantidad significativamente mayor de gutapercha/sellador que los instrumentos PWOG en conductos curvos simulados. Sin embargo, ninguno de los instrumentos ensayados removió completamente los materiales de obturación.

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The choice of the material used to fill screw access channels in implant-supported prostheses depends, in most cases, on operator's preference, without considering the susceptibility of biofilm colonization. Therefore, the aim of this study was to determine and compare the total amount of biofilm formed on different materials used to fill screw access channels in implant abutments. For this propose, titanium implant analogs were attached on abutments and divided into 5 groups: positive control (no filling material); negative control (closed with resin); and filled with cotton, gutta-percha, or polytetrafluoroethylene (PTFE). The analogs with attached abutments were then immersed in a brain heart infusion medium containing Candida albicans (strain 10231 from American Type Culture Collection [ATCC]) and incubated aerobically at 37°C with gentle agitation. After 15 days, materials were removed, and total viable biofilm on each material was quantified by methyl tetrazolium reduction assay at 490 nm. All experiments were performed in triplicate. Data were processed by IBM SPSS Statistic software using 1-way analysis of variance and Bonferroni post hoc tests to analyze differences between groups, with an overall significance level of P < .001. A significant difference was observed between cotton and gutta-percha (P < .017) and between cotton and PTFE (P < .025). However, there was no statistical difference between gutta-percha and PTFE (P > .050). Thus, this in vitro experiment showed that gutta-percha and PTFE presented lower biofilm formation compared with cotton when used to fill screw access channels. These results can provide a basis for future clinical studies that can be a guide to decreasing the occurrence of gaps and bacterial growth inside the implant/abutment attachment site. In addition, controlled in vivo studies are necessary to confirm the clinical viability of findings of this study.

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AIM: To assess the interfacial adaptation (gap-containing areas) of two root canal sealers (EndoSequence BC Sealer and AH Plus) to a conventional gutta-percha (GP) cone (GP-sealer interface) in oval-shaped canals filled using the single cone technique. METHODOLOGY: Sixteen teeth with oval-shaped canals were selected, scanned in a micro-computed tomographic device, and pair-matched according to the volume, aspect ratio and configuration of root canals. Root canals were then sequentially prepared with WaveOne Gold Primary and Large instruments, followed by filling with WaveOne Large GP points associated with either the premixed calcium-silicate EndoSequence BC Sealer or the epoxy resin-based AH Plus sealer (n = 8 per group) using the single cone technique. After 7 days stored in phosphate-buffered saline solution at 37°C, the specimens were rescanned and the reconstructed images segmented in order to differentiate the filling materials (sealer and GP cone) to the dentine. A total of 453 cross-sectional slices were assessed and categorized according to the presence or absence of gaps at the GP-sealer interface. Mann-Whitney U-test verified the differences between groups and were considered significant at alpha = 5%. RESULTS: Gaps were non-homogenously distributed in two-dimensional axial cross-section images and none of the specimens showed completely gap-free areas along the entire GP-sealer interface. Root canals filled with EndoSequence BC Sealer and AH Plus displayed 171 (37.75%) and 136 (30.02%) slices with gaps in the GP-sealer interface and these frequencies were statistically significant (p = .000). CONCLUSIONS: Although none of the specimens had a gap-free area along the entire GP-sealer interface, oval canals filled with AH Plus showed less gaps than the ones filled with EndoSequence BC Sealer.

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ABSTRACT The aim of this study was to compare the capacity of two reciprocating NiTi instruments in removing gutta-percha/sealer material from simulated curved root canals (SCRC). The time required for filling material removal was also recorded. Twenty SCRCs were divided into two groups of 10 (n=10) samples each. In Group 1, the SCRC were prepared to a R25 Reciproc Blue instrument (RCPB; VDW, Munich, Germany). In Group 2 the SCRC were prepared to a Primary WaveOne Gold instrument (PWOG; Dentsply, Ballaigues, Switzerland). In both groups, the canals were filled with matched-taper single gutta-percha cones and AH Plus sealer. Filling materials were removed with R25 RCPB (Group 1) and PWOG (Group 2). The amount of remaining gutta-percha/sealer was calculated at three predetermined levels of evaluation located at 2, 6 and 10 mm from the WL and expressed in percentages. Canals re-treated with RCPB contained significantly less remaining gutta-percha/sealer compared to canals prepared with PWOG (P=0.02). The RCPB instruments required significantly less time to complete the retreatment procedures (P<0.01). No unwinding or instrument separation was noted. RCPB instruments removed significantly more gutta-percha/sealer from simulated curved root canals than PWOG. However, neither of the tested instruments completely removed all filling materials.

RESUMEN El propósito del presente estudio fue comparar la capacidad de dos instrumentos de NiTi de movimiento reciproco para remover la obturación de gutapercha/sellador durante el retratamiento de conductos curvos simulados (SCRC). El tiempo requerido para la remoción del material fue también registrado. Se utilizaron veinte (n=20) SCRC divididos en dos grupos de diez especimenes (n=10) cada uno. En el Grupo 1 los SCRC se prepararon hasta un instrumento Reciproc Blue R25 (RCPB; VDW, Munich, Germany). En el Grupo 2, los SCRC se prepararon hasta un instrumento WaveOne Gold Primary (PWOG; Dentsply, Ballaigues, Switzerland). En ambos grupos los conductos se obturaron con cono único de gutapercha de conicidad creciente y el sellador AH Plus. La remoción de los materiales de obturación se realizó mediante los instrumentos RCPB R25 (Grupo 1) o PWOG (Grupo 2). La cantidad de gutapercha/sellador remanente se calculó en tres niveles de evaluación predeterminados ubicados a 2, 6 y 10 mm de la LT, y finalmente fue expresada en porcentajes. La cantidad de gutapercha/ sellador remanente en los SCRC retratados con RCPB fue significativamente menor en comparación con los que fueron retratados con PWOG (P=0.02). Los instrumentos RCPB requirieron un tiempo significativamente menor para completar el retratamiento (P<0.01). No se observaron deformaciones o separación de los instrumentos. Los instrumentos RCPB removieron una cantidad significativamente mayor de gutapercha/sellador que los instrumentos PWOG en conductos curvos simulados. Sin embargo, ninguno de los instrumentos ensayados removió completamente los materiales de obturación.

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AIM: To assess the influence of remnants of filling material on hydroxyl ion diffusion from calcium hydroxide (CH) paste, measured by the pH value, in retreated teeth. MATERIALS AND METHODS: A total of 120 single-rooted extracted teeth were prepared up to a size 35 hand file and filled. For retreatment, the specimens were divided into four groups (n = 20): ProTaper Universal Retreatment (PUR), PUR with additional instrumentation (PURA), Mtwo Retreatment (MTWR), and MTWR with additional instrumentation (MTWRA). Negative (NEG) and positive (POS) control groups were composed by 20 specimens each one. The specimens, except NEG, were filled with CH paste. The retreated groups were scanned using cone-beam computed tomography (CBCT) for analysis of filling remnants. The pH assessment was performed at the baseline, after seven, 21, 45, and 60 days of immersion in saline. Data were analyzed using Shapiro-Wilk and Levene's test, followed by a two-way analysis of variance (ANOVA) and Tukey's test. RESULTS: Additional instrumentation (PURA and MTWRA) were superior regarding removal of the filling material (p <0.05); however, without significant difference (p >0.05). The mean pH value in all groups increased (p <0.05). After 60 days, no statistical difference was observed among POS and PURA; and MTWR and MTWRA. There was less diffusion of hydroxyl ions when the amount of remnants was greater than 59%. CONCLUSION: Additional instrumentation improved the ability to remove filling material in both systems. All groups presented increasing pH; however, the higher the amount of remnants, the lower the diffusion of hydroxyl ions. CLINICAL SIGNIFICANCE: The amount of remnants allows less diffusion of calcium hydroxyl ions. Thus, additional instrumentation improves the ability to remove these materials.

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Aim:To demonstrate the effectiveness of disinfecting substances with 2% and 5% Sodium Hypochlorite and 2% Chlorhexidine Gluconate at each of the pre-established times of 0:15 and 0:30 seconds, and 1, 2, 5, and 10 minutes. Methods: This study selected 96 gutta-percha cones that were contaminated with Enterococcus Faecalis, dried and treated with the aforementioned substances and applied at pre-established times. Subsequently, these were transferred to sterile Brain Heart Infusion broth and placed in a bacteriological incubator at 37°C for 24 hours to evaluate microbial growth, as well as in a nutrient agar medium in Petri dishes. Half of the cone was transferred to individual filter paper packages and exposed to the environment in a dental clinic at Universidade José do Rosário Velano, for 7 days, with subsequent evaluation for microbial growth. The bacterial phenotype test was performed using Gram stain and growth in 6.5% saline solution. The results were submitted to statistical analysis using the Kruskal Wallis H test, with a significance level of 5%. Results:The substances were effective at all times tested and individual storage supported disinfection. In the statistics test, the p-value was greater than 0.05, as there was no variability in the data configurations. Conclusion: The disinfection of gutta-percha cones and individual storage was an effective protocol to be adopted with 2% and 5% Sodium Hypochlorite and 2% Chlorhexidine.

Objetivo: Demonstrar a eficácia de substâncias desinfetantes, Hipoclorito de Sódio a 2% e 5% e Gluconato de Clorexidina 2% em cada um dos tempos pré-estabelecidos de 15 e 30 segundos, e 1,2, 5 e 10 minutos.Métodos: Este estudo selecionou 96 cones de guta-percha,contaminados com Enterococcus Faecalis, secos e tratados com as substâncias citadas e aplicadas em tempos pré-estabelecidos. Posteriormente, estes foram transferidos para tubos contendo caldo Infusão Cérebro Coração estéril e colocados estufa bacteriológica a 37°C por 24 horas para avaliar o crescimento microbiano, também verificado em meio ágar nutriente em Placas de Petri. Metade dos cones foram transferidos para embalagens individuais de papel de filtro, e expostas ao ambiente da clínica odontológica da Universidade José do Rosário Velano por 7 dias, com posterior avaliação do crescimento microbiano. O teste do fenótipo bacteriano foi realizado pela coloração de Gram e crescimento em solução salina a 6,5%. Os resultados foram submetidos à análise estatística por meio do Teste H de Kruskal Wallis, com nível de significância de 5%.Resultados: As substâncias foram eficazes em todos os tempos testados e o armazenamento individual favoreceu a desinfecção. No teste estatístico, o valor de p foi maior que 0,05, pois não houve variabilidade nas configurações dos dados.Conclusão: A desinfecção dos cones com Hipoclorito de Sódio 2% e 5% e Clorexidina 2% a partir de 15 segundos, e o armazenamento individual foram protocolos eficazes para serem adotados.

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The objective of this research was to compare the solvent action of orange oil, eucalyptol and experimental lemongrass oil on gutta-percha cones for cold technique and thermoplastic technique. Methodology: The sample consisted of 80 gutta-percha cones No. 80 Endomedic and F3 ProTaper Universal. The cones were divided into 4 groups (n = 20) according to the type of solvent, and each one was divided into two subgroups (n = 10) according to the exposure time (5 min and 10 min). The cones were immersed in the respective solvent for 5 or 10 minutes. Then, they were washed with 5 ml of alcohol for 5 minutes and rinsed with water. Finally, after drying at room temperature for one hour, the solvent action was recorded in grams (g) of weight loss. The results were analyzed and compared using Tukey's test (p<0.05). Results: The Endomedic cone exposed for 5 min to eucalyptol obtained the lowest average weight (0.0625 ± 0.0009 g), followed by Lemongrass oil (0.0629 ± 0.0003 g) and orange oil (0.0629 ± 0.0010 g), although not a significant difference (p > 0.05) was found between each one. Conclusion: The experimental solvent of Lemongrass, eucalyptol and orange oil presented a similar solvent effect on the guta-percha cone for cold and thermoplastic technique. At a clinical level, the three solvents studied could be used in endodontic retreatment as alternative solutions with a similar solvent effect.

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Introdução: Os cones de guta percha são utilizados para obturação de canais radiculares, entretanto a desinfecção desses cones deve ser realizada previamente à obturação, evitando uma contaminação, uma vez que com o manuseio diário na clínica, pode ocorrer a proliferação de microrganismos. Objetivo: Relatar através de uma revisão de literatura a importância da desinfecção de cones de guta percha previamente á obturação dos canais radiculares, com diferentes soluções desinfetantes utilizadas na odontologia. Metodologia: Foi realizada uma busca em artigos de revisão de literatura nas bases de dados Pubmed, Scielo, Lilacs com diferentes anos de publicação, mas todos com enfoque principal no tema de desinfecção de cones de guta percha com diferentes soluções químicas. Foram utilizados os descritores guta percha, desinfecção, soluções químicas. Conclusão: Medidas de prevenção devem ser empregadas durante o atendimento odontológico, em especial durante a obturação dos canais radiculares, sendo a desinfecção dos cones de guta percha essencial(AU)

Introduction: Gutta percha points are used to fill root canals, however its disinfection must be performed prior to filling, avoiding contamination, since with daily handling in the proliferation of microorganisms can occur. Objective: To report through a literature review the importance of disinfecting gutta percha points prior to root canal filling, with different disinfectant solutions used in dentistry. Methodology: A search for literature review articles was carried out in the Pubmed, Scielo, Lilacs databases with different years of publications, but all with a main focus on the topic of disinfection of gutta-percha points with different chemical solutions. The keywords gutta percha, disinfection, chemical solutions were used. Conclusion: Preventive measures must be used during dental care, especially during root canal filling, with the disinfection of gutta percha points being an essential(AU)

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Foreign bodies in the ethmoid sinus are uncommon, and endodontic gutta percha displacement is even more rare. We present and discuss a case of displacement of gutta percha into the ethmoid sinus following root canal intervention of a maxillary first molar which presumably migrated through the maxillary sinus. There have been reports on the displacement of tooth roots or implants into the maxillary sinus. However, we know of only one such report on the migration of a gutta-percha point into the ethmoid sinus.

Los cuerpos extraños en el seno etmoidal son infrecuentes y el desplazamiento de la gutapercha endodóntica es aún más raro. Presentamos y analizamos un caso de desplazamiento de la gutapercha hacia el seno etmoidal después de la intervención del conducto radicular de un primer molar superior que se presume migró a través del seno maxilar. A pesar de que existen informes sobre el desplazamiento de raíces dentales o implantes hacia el seno maxilar, solo conocemos un informe de este tipo sobre la migración de un punto de gutapercha hacia el seno etmoidal.

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Objetivo: O objetivo do presente estudo é revisar a literatura sobre os tipos de guta-percha para obturação, as suas formas de apresentação e as características físico-químicas e biológicas do material. Métodos: Artigos publicados entre 1973 e 2016 foram selecionados nas bases de dados PubMed e Science Direct. Foram utilizados como descritores na língua inglesa "gutta-percha and obturation"; "cones of gutta-percha" e "obturation cones and endodontic". Revisão: A guta-percha é um material de origem orgânica que pode ser encontrado em duas formas cristalinas. Encontra-se, geralmente, na forma de cones compostos de guta-percha associada a outras substâncias. A guta-percha é considerada inerte e biocompatível, porque causa pouca reação alérgica e possui biodegradação lenta. Pode sofrer alterações físico-químicas quando utilizada em técnicas de termo-obturação, nas quais as altas temperaturas aceleram a sua degradação, comprometendo a sua função. Além disso, a sua degrada- ção precoce é também afetada pela presença ou ausência de lesões periapicais. Conclusão: Os cones de guta-percha ainda são considerados o material mais adequado e acessível para o preenchimento de canais radiculares (AU).

Objective: The objective of this study was to identify the types of gutta-percha for obturation and their forms of presentation, as well as physical-chemical and biological characteristics of the material. Materials and Methods: Articles were selected from the PubMed and Science Direct databases, published between 1973 and 2016. Were used as descriptors: "gutta-percha and obturation"; "cones of gutta-percha" and "obturation cones and endodontic". Review: Gutta-percha is a material of organic origin, which can be found in two crystalline forms. It is usually found in the form of cones composed of gutta-percha associated with other substances. Gutta-percha is considered inert and biocompatible, because it causes little allergic reaction and slow biodegradation. It can suffer physical-chemical changes when used in thermo-obturation techniques, where the high temperatures accelerate its degradation, compromising its function. In addition, its early degradation is also affected by the presence or absence of periapical lesions. Conclusion: Gutta-percha Cones are still considered the most suitable and accessible material for root canal filling

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AIM: To compare the bond strength of an epoxy resin-based sealer and two calcium silicate-based sealers (CSS) to gutta-percha discs using a new method. METHODOLOGY: Round discs of gutta-percha (n = 60), measuring 10 mm in diameter and 2 mm in thickness, were placed on a glass plate and a drop of each sealer (AH Plus, EndoSequence BC Sealer and EndoSeal MTA) was placed on their surface. Another identical disc was placed onto the first one and a standardized weight (0.0981 N) applied over them using a specially developed apparatus. Ten samples prepared for each sealer were submitted to a microshear bond strength test accomplished by a specially designed set-up coupled to a universal testing machine. The Kruskal-Wallis test followed by a post hoc procedure was used to compare groups considering the preliminary analysis of the raw data had indicated the nonadherence to a Gaussian distribution (Shapiro-Wilk, p < .05). Alpha error was set at 5%. RESULTS: Overall, no premature failure occurred. All sealers had some degree of adhesiveness to gutta-percha discs but with a significant difference amongst them (Kruskal-Wallis, p = .019). The epoxy resin-based sealer (AH Plus) had significantly higher median shear bond strength values (1.43 MPa; 1.40-1.83) compared to EndoSeal MTA (0.53 MPa; 0.46-0.73) (p = .021) and EndoSequence BC Sealer (0.45 MPa; 0.34-0.46) (p = .023), whilst the lowest median value was observed with EndoSequence BC Sealer (0.45 MPa; 0.34-0.46) (p < .05). CONCLUSIONS: CSS sealers had weaker bonding to gutta-percha when compared to the epoxy resin-based AH Plus sealer. The proposed methodology is an innovative and reproducible method for testing the bond strength of root canal sealers to gutta-percha.

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