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The genus Elasmoderus belongs to the family Tristiridae, an orthopteran group that is distributed across the transitional region of South America and represented in the Andean areas of Peru, Argentina, and Chile. The species of Elasmoderus have morphological adaptations that make them especially suited for surviving in extreme arid environments and are an endemic and eremic group of the north-central region of Chile. On the basis of field samplings, direct observations, and a comprehensive literature review, we collected information about the genus Elasmoderus. The objectives of this research were: (i) to provide updated information on the geographical distribution range of the species of the genus Elasmoderus, and (ii) to collect and synthesize the most relevant information on the natural history and ecology of this orthopteran group as a basis for future long-term studies of its populations. Although this genus is underrepresented compared to other orthopteran families, it has developed interesting adaptations to extreme arid environments that remain unstudied to this date. E. lutescens is known to be one of the species with a wider latitudinal distribution, mostly on the coast and longitudinal valley of Atacama. E. minutus has a more restricted distribution, whereas E. wagenknechti is concentrated in coastal and interior environments of the Coquimbo region, an area where it reaches high population densities, thus affecting crops and natural vegetation and polluting water sources. Our findings underscore the importance of conducting comprehensive research on native insect groups that are poorly known but crucial for arid and semiarid ecosystems. These data will serve as a starting point for conducting long-term studies on this orthopteran group to gain a better understanding of the importance and role of these species in the semiarid ecosystems of northern Chile.

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In recent times, insects have gained attention because of their nutritional characteristics as well as the environmental advantages of their production. In this research, the effect of the diet of grasshoppers (Sphenarium purpurascens) under controlled conditions on their chemical and nutritional content was studied. The insects were divided into two groups: maize leaf-fed grasshoppers (MFG) and soy sprout-fed grasshoppers (SFG). To evaluate the changes in composition, chemical analysis (protein, fiber, fat, ashes, and chitin) was carried out in triplicate according to AOAC procedures, and a Student's t-test was used to determine any significant differences. The results showed a higher content of crude protein, in vitro protein digestibility percentage, and sum of non-essential amino acids (NEAAs) in the MFG samples compared with the SFG samples. The total dietary fiber, insoluble dietary fiber, soluble dietary fiber, sum of the EAA, non-essential amino acid percentage (EAA%), and biological value percentage (BV%) were higher in the SFG than the MFG, while in the amino acid profile and chitin content, no significant differences were obtained, although an increase in oleic acid in the SFG was observed. In FTIR, a ß-sheet appeared in the SFG, which could be related to the low in vitro protein digestibility. The use of a soy sprout diet caused changes in the chemical composition and nutritional content of grasshoppers. This represents an opportunity to improve their nutritional value for commercial interests.

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Chromosomal inversions are known to play roles in adaptation and differentiation in many species. They involve clusters of correlated genes (i.e., loci in linkage disequilibrium, LD) possibly associated with environmental variables. The grasshopper "species complex" Trimerotropis pallidipennis comprises several genetic lineages distributed from North to South America in arid and semi-arid high-altitude environments. The southernmost lineage, Trimerotropis sp., segregates for four to seven putative inversions that display clinal variation, possibly through adaptation to temperate environments. We analysed chromosomal, mitochondrial and genome-wide single nucleotide polymorphism data in 19 Trimerotropis sp. populations mainly distributed along two altitudinal gradients (MS and Ju). Populations across Argentina comprise two main chromosomally and genetically differentiated lineages: one distributed across the southernmost border of the "Andes Centrales," adding evidence for a differentiation hotspot in this area; and the other widely distributed in Argentina. Within the latter, network analytical approaches to LD found three clusters of correlated loci (LD-clusters), with inversion karyotypes explaining >79% of the genetic variation. Outlier loci associated with environmental variables mapped to two of these LD-clusters. Furthermore, despite the complex geographical history indicated by population genetic analyses, the clines in inversion karyotypes have remained stable for more than 20 generations, implicating their role in adaptation and differentiation within this lineage. We hypothesize that these clines could be the consequence of a coupling between extrinsic postzygotic barriers and spatially varying selection along environmental gradients resulting in a hybrid zone. These results provide a framework for future investigations about candidate genes implicated in rapid adaptation to new environments.

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The Batrachideinae subfamily is a well-defined and taxonomically stable taxon, with a pantropical distribution and an important representation in the Neotropical region. In this study, the characters and distributions of the genera and species are discussed based on a morphological analysis and a key to genera of the American Batrachideinae is also provided. New synonymies are established: Tettigidea Scudder, 1862 = Eotetrix Gorochov, 2012 syn. nov.; Batrachidea mucronata (Saint-Fargeau Serville, 1825) = Tettigidea granulosa Bruner, 1913 syn. nov.; Batrachidea planus (Hancock, 1907) = Batrachidea brevis (Hancock, 1909) syn. nov.; Tettigidea lateralis (Say, 1824) = Tettigidea nicaraguae Bruner, 1895 syn. nov.; Tettigidea lateralis (Say, 1824) = Tettigidea annulipes Bruner, 1910 syn. nov.; Tettigidea cuspidata Scudder, 1875 = Tettigidea corrugata Bruner, 1910 syn. nov.; Tettigidea cuspidata Scudder, 1875 = Tettigidea multicostata Bolívar, 1887 syn. nov.; Tettigidea chapadensis Bruner, 1910 = Tettigidea costalis Bruner, 1910 syn. nov.; Tettigidea chapadensis Bruner, 1910 = Tettigidea hancocki Bruner, 1910 syn. nov.; Tettigidea intermedia Bruner, 1910 = Tettigidea subatera Bruner, 1910 syn. nov.; Tettigidea scudderi Bolívar, 1887 = Tettigidea steinbachi Bruner, 1920 syn. nov. Three species are transferred from the genus Tettigidea to the genus Paxilla Bolívar, 1887: Paxilla mexicana (Hancock, 1915) comb. nov., Paxilla tecta (Morse, 1900) comb. nov., Paxilla nigra (Morse, 1900) comb. nov.; Tettigidea unicornis (Gorochov, 2012) comb. nov. is transferred from the genus Eotetrix Gorochov, 2012 to Tettigidea, and Batrachidea planus (Hancock, 1907) comb. nov. from Tettigidea to Batrachidea Serville, 1838. Five new species groups (lateralis, armata, cuspidata, paratecta and scudderi) are recognized in the genus Tettigidea. Neotype of Tettigidea lateralis lateralis is designated. Keys to the Batrachideinae genera and species of the most diverse American genera (Tettigidea and Paxilla) are also provided.

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The present study deals with the taxonomical analysis of the edible insects commercialized in the state of Morelos, Mexico. We have recorded two species under the order Orthoptera and four species under Hemiptera-Heteroptera. Our work revealed that grasshoppers and small hemipterans known as 'jumiles' are the two main insects sold in the markets of the state of Morelos. We also discuss insects' prices and their economic importance for the livelihood improvement of the local people connected to the edible insect trade.

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Abstract Borellia bruneri, a common grasshopper in much of the grasslands of Argentina and Uruguay, is considered, according to the categories widely accepted for defining the pest status of grasshopper species, a "Frequent plague of importance". In order to determine fundamental aspects of its biology and reproduction, three cohorts of B. bruneri were monitored under controlled conditions (30º C, 14L: 10D, 40% RH). The total duration of nymphal development was 50.6 days, both males and females having five nymphal instars. There was a significant difference in the duration of the different stages within each cohort. In the three cohorts, the first instar duration (12.87 days) was longer than the rest, approximately 5.6 days more than the second that was the shortest (7.26 days). The average longevity of female adults was 56.6 days, and in males, 54.4 days. The number of egg-pods per female was 3.5 and the amount of eggs per egg-pod was 10.8. Mean fecundity was 37.9 eggs per female with an oviposition rate of 1.20 eggs/female/day. Finally, knowing the life cycle of B. bruneri is relevant in order to optimize the control measures for this species.

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To be able to foretell which species will be at risk of extinction from possible expansion of agriculture into natural areas, we need to determine how land use affects animal groups especially insects with different life history traits. Intuitively, we can predict that the proportion of specialist versus generalist grasshoppers and widespread versus localised species can be an indicator of change in vegetation patterns in a landscape. As a result, the study examined the relationship between the extent of range distribution and mobility of grasshoppers and land-use type in the Cape Floristic Region (CFR) biodiversity hotspot. It aimed at examining grasshopper assemblage composition in association with different land uses. We specifically related the number and type of sites occupied by grasshopper species to their mobility and distribution type to find out which characters improve species survival under highly modified habitats. Grasshopper assemblages were dominated by highly mobile generalists that tolerated transformed landscapes, and mostly geographically widespread. This contrasts with the low- to medium-mobile, range-restricted specialists, which make up close to 30% of the total collection, with a lower tolerance for anthropogenically modified landscapes in the CFR. This study also reveal that the fynbos biome is an important habitat for low-mobility and range-restricted species, and hence require conscious conservation efforts to conserve such species to prevent possible future biotic erosion and homogenisation in this hotspot. This result can be used to assess the possible extent of biotic erosion or otherwise in order to recommend better conservation efforts in the CFR.

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A checklist of the type specimens of 273 species of Caelifera (Orthoptera) deposited in the Museu Nacional, Universidade de Rio de Janeiro (MNRJ), Rio de Janeiro, Brazil is provided.

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Species of Temnomastax have wide morphological similarities, and differentiation is usually based on male and female genitalia. In this study, we tested whether morphometric differences contribute to differentiation of species of Temnomastax, proposed an identification key for males, and morphometrically studied the sexual dimorphism of Temnomastax hamus and Temnomastax ricardoi. Analysis was performed using 204 specimens belonging to six species; fifteen morphometric variables were used. We used MANOVA and ANOVA to test the morphometric differences among species and Discriminant Analysis for sexual distinction. Temnomastax sp. nov. 1 had the highest values in ⅓ of all analyzed variables used for morphometric distinction of males, followed by Temnomastax latens, T. ricardoi and Temnomastax sp. nov. 2, and T. hamus and Temnomastax tigris. Two groups were formed by the MANOVA, with length of body, length of hind femur, and length of tegmen as the main variables that distinguished them. Overall, females of T. hamus and T. ricardoi presented higher averages for measurement values used in morphometric distinction between sexes than conspecific males and based on discriminant analysis we found significant differences between sexes. There are significant differences in morphometric variations and the ratio between body length and tegmen length provided reliable evidence for differentiation among species, which can be used as a valid tool that complements the identification of Temnomastax species.

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Identification Orphulellini grasshoppers (Acrididae: Gomphocerinae) species has been difficult due to high polymorphism rate. Orphulella Giglio-Tos, 1894 is a genus with widespread geographical distribution and poor descriptions. Orphulella punctata (De Geer, 1773) has an extensive record of occurrence and available information about the phallic complex, however, there is poor data describing other parts of the male reproductive tract. The objective of this study was characterizes the internal organs of the male reproductive system and spermatozoa of O. punctata. Orphulella punctata testes are of Fountain type, each having only four follicles. Spermatozoa into the seminal vesicle are arranged in bundles with c.a. 2320 µm length, with a nucleus 110 µm long. The spermatozoa are covered by a glycocalyx, the nucleus is cylindrical with condensed chromatin and connected to the flagellum by a dense and lamellar centriole adjunct. The axoneme have 9 + 9 + 2 pattern and present two symmetrical mitochondrial derivatives. A fibrous net and two flat membranous cisternae fill the space between the axoneme and mitochondrial derivatives. This is the first description of the reproductive system of a Gomphocerinae representative.

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Temnomastax spielmanni Olivier, 2014 n. syn. is proposed as new synonym of Eumastacops nemorivaga Rehn & Rehn, 1942. New distribution records in Peru and Colombia are provided for the following four species: Eumastax nigrovittata Descamps, 1979; Paramastax nigra (Scudder, 1875); Pseudomastax nigroplagiata Descamps, 1970; and Pseudomastax personata (Bolívar, 1881). New information on the distribution of Paramastax flavovittata Descamps, 1973 and E. nemorivaga is added, resulting in the expansion of the geographic distribution. Photographs of all studied species are provided.

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In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3 (+) blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3 (+) blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2, S9 and S10 autosomes in R. nordestinum. In R. mariajoseae, the 5S rDNA sites were localized in the in L1 and L2 bivalents and in the X chromosome. In R. nordestinum, the 5S genes were located in the L2, L3, M4 and M5 pairs. In both species the histone H4 genes were present in a medium size bivalent. Together, these data evidence a great variability of chromosome markers and show that the 18S and 5S ribosomal genes are dispersed in the Radacridium genome without a significant correlation.

RESUMO

In Argentina, the grasslands of Pampas region comprise approximately 15% of the country. As in other grasslands of the world, grasshoppers are among the most important native herbivores. Their economic importance has been recognized in Argentina since the mid to late nineteenth century, since outbreaks of different species have become recurrent phenomena. Therefore, the main objective of this work was to study their diversity and distribution in grasslands of the Southern Pampas region (Laprida county, Buenos Aires province), as one of the most affected areas. The study was conducted during five seasons (2005-10). Sampling sites were represented by the most common plant communities in this area, classified in four categories: native grasslands, disturbed grasslands, implanted pastures and halophilous grasslands. The samplings were conducted from mid-spring to early autumn, with five or six samples per season. We estimated the following population descriptors: species richness (S), eveness (E), dominance (J), and diversity index (H´). In order to evaluate the similitude of the grasshopper communities present in the different plant communities, we used qualitative and quantitative coefficients of similitude. A total of 22 species of grasshoppers were collected, of which 21 belong to the family Acrididae. The subfamily Melanoplinae was the most diverse with eight species. The largest species richness was recorded in native grasslands (18). The different communities of grasshoppers had similar indices of evenness and dominance (p>0.05). Considering all plant communities, the average value of Shannon-Wiener index was 1.58±0.075. There was a positive correlation between evenness index and species richness (p<0.05). The diversity index H’ was different between plants communities (p<0.05), and it was higher in the disturbed grassland (1.75±0.096, p<0.05) than in the halophilous grasslands (1.34±0.12). Native and disturbed grasslands had a higher plant richness than halophilous grasslands and implanted pastures (p<0.05). There was a positive relationship between plant richness and grasshoppers species richness, and diversity of grasshoppers. According to the qualitative indices applied, the similitude between different grasshopper communities was higher than 60%. In general, the species that had a higher frequency of occurrence showed greater abundance and distribution. Covasacris pallidinota, Dichroplus elongatus, D. maculipennis, Borellia bruneri and B. pallid were the most widely distributed species, most of them (12) showed a restricted distribution and few (five) an intermediate distribution.

Los acridios son componentes nativos de los pastizales, dichos sistemas han sido modificados debido a la intensificación de las actividades agrícola-ganaderas y por lo tanto se considera importante estudiar las comunidades de acridios asociadas. Se estudió la diversidad y distribución de acridios en el Sur de la región pampeana a través de muestreos realizados en las comunidades vegetales más representativas del partido de Laprida, provincia de Buenos Aires, durante cinco temporadas (2005-2010). Se recolectaron 22 especies. La subfamilia Melanoplinae fue la más diversa (ocho especies). La mayor cantidad de especies se observó en los pastizales nativos (18). La diversidad en los pastizales alterados (1.75±0.096) fue superior (p<0.05) a la de los pastizales halófilos (1.34±0.12). La riqueza de plantas en los pastizales nativos y los pastizales alterados fue superior (p<0.05) a la de las demás comunidades. Se registro una relación positiva entre la riqueza de plantas y las variables riqueza y diversidad de acridios. La similitud entre las comunidades de acridios estimada por los índices cualitativos fue superior al 60%. En general, las especies que tuvieron una mayor frecuencia de aparición, mostraron una mayor abundancia y distribución. Covasacris pallidinota, Dichroplus elongatus, D. maculipennis, Borellia bruneri y B. pallida fueron las de mayor distribución, cinco especies tuvieron una distribución intermedia y la mayoría (12) una distribución restringida.

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Variação do peso fresco em Cornops aquaticum (Bruner) (Orthoptera, Acrididae) associado a Eichhornia azurea (Sw) Kunth (Pontederiaceae) em uma baía no Pantanal de Poconé, Mato Grosso. Cornops aquaticum (Bruner, 1906) (Orthoptera, Acrididae) desenvolve seu ciclo de vida sobre macrófitas aquáticas da família Pontederiaceae. Como os gafanhotos são capazes de responder às mudanças sazonais, a alternância de períodos que ocorre no Pantanal pode refletir em sua biologia. Este estudo foi desenvolvido no Pantanal de Poconé - MT, com o objetivo de avaliar possíveis variações no peso fresco dos adultos e ninfas de C. aquaticum. Durante o período de março/2006 a fevereiro/2007 coletaram-se mensalmente, 50 indivíduos de C. aquaticum. Um total de 600 indivíduos foi avaliado, sendo 43,5 por cento adultos e 56,5 por cento ninfas. Os maiores valores de peso fresco total ocorreram nos meses de setembro (9,106g; 0,182g/indivíduo) e outubro/2006 (8,865g; 0,177g/indivíduo) e os menores em março/2006 (3,413g; 0,068g/indivíduo). Nos indivíduos adultos os maiores pesos frescos foram registrados em setembro/2006 (8,680g; 0,223g/indivíduo) e outubro/2006 (8,654g; 0,234g/indivíduo), no final do período de seca, e o menor em março/2006 (1,792g; 0,138g/indivíduo), durante o período de cheia. As ninfas tiveram o maior peso fresco em abril/2006 (2,913g; 0,076g/indivíduo) início da vazante, enquanto o menor peso fresco ocorreu em outubro/2006 (0,211g; 0,016g/indivíduo) início da enchente. Apenas a variação no peso fresco médio das fêmeas foi significativa (f = 6,43; p = 0,001), com os maiores registros durante o período de enchente, o que pode evidenciar uma estratégia reprodutiva.

Cornops aquaticum (Bruner, 1906) (Orthoptera, Acrididae) develops its life cycle on aquatic macrophyte of the Pontederiaceae family. As grasshoppers are able to respond to the seasonal changes, the alternation of periods that occurs in the Pantanal may reflect in their biology. This study was carried out in the Pantanal of Poconé- MT, with the aim of evaluating possible variations in the fresh weight of the adults and nymphs of C. aquaticum. From March/2006 to February/2007, 50 individuals of C. aquaticum were collected each month. A total of 600 individuals was assessed, 43.5 percent of which were adults and 56.5 percent nymphs. The highest values of total fresh weight occurred in September (9.106g; 0.182g/individual) and October/2006 (8.865g; 0.177g/individual) and the lowest in March/2006 (3.413g; 0.068g/individual). Among the adult individuals the highest fresh weight was registered in September/2006 (8.680g; 0.223g/individual) and October/2006 (8.654g; 0.234g/individual), at the end of the dry period, and the lowest in March/2006 (1.792g; 0.138g/individual) during the flood period. The nymphs had the highest fresh weight in April/2006 (2.913g; 0.076g/individual) at the beginning of the draining period, whereas the lowest fresh weight occurred in October/2006 (0.211g; 0.016g/individual) at the beginning of the flood period. Only the variation in the average fresh weight of females was significant (f = 6.43; p = 0.001) with the highest recordings occurring during the flood period, what may show a reproductive strategy.

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A cladistic analysis of the relationships among the species of the South American genera of the group Sitalces (Sitalces Stål, 1878, Eusitalces Bruner, 1911, Parasitalces Bruner, 1911, Psilocirtus Bruner, 1911, Liebermannacris Costa & Carvalho, 2006, Robustusacris Costa & Carvalho, 2006, Arimacris Costa & Carvalho, 2006, Salvadoracris Costa & Carvalho, 2006 and Caruaruacris Costa & Carvalho, 2006) is presented. The analysis included 14 species with three outgroups and 34 morphological characters. The monophyly of the group is supported by three sinapomorphies with 100% consistency and retention indices: prozona larger than the metazona, posterior border of the pronotum not angulated and supranal plate shorter than cerci. The analysis resulted in a single cladogram: (P. olivaceus (E. vittatus (L. dorsualis; L. punctifrons) (R. balzapambae (A. trinitatis (S. nigritus; C. bivittatus) (S. volxemi (P. vulneratus; P. sexnotata)))))). Biogeographic comments are presented and related with pre-established standards.

Uma análise cladística do relacionamento entre as espécies sul-americanas do grupo Sitalces (Sitalces Stål, 1878, Eusitalces Bruner, 1911, Parasitalces Bruner, 1911, Psilocirtus Bruner, 1911, Liebermannacris Costa & Carvalho, 2006, Robustusacris Costa & Carvalho, 2006, Arimacris Costa & Carvalho, 2006, Salvadoracris Costa & Carvalho, 2006 e Caruaruacris Costa & Carvalho, 2006) é apresentada. A análise incluiu 14 espécies com três grupos-externos e 34 caracteres morfológicos. A monofilia do grupo é suportada por três sinapomorfias com índices de consistência e retenção de 100%: prozona maior que a metazona, borda posterior do pronoto não angulada e placa supranal mais curta em relação aos cercos. A análise resultou em um único cladograma: (P. olivaceus (E. vittatus (L. dorsualis; L. punctifrons) (R. balzapambae (A. trinitatis (S. nigritus; C. bivittatus) (S. volxemi (P. vulneratus; P. sexnotata)))))). Comentários biogeográficos são apresentados e relacionados com padrões pré-estabelecidos.

RESUMO

A cladistic analysis of the relationships among the species of the South American genera of the group Sitalces (Sitalces Stål, 1878, Eusitalces Bruner, 1911, Parasitalces Bruner, 1911, Psilocirtus Bruner, 1911, Liebermannacris Costa & Carvalho, 2006, Robustusacris Costa & Carvalho, 2006, Arimacris Costa & Carvalho, 2006, Salvadoracris Costa & Carvalho, 2006 and Caruaruacris Costa & Carvalho, 2006) is presented. The analysis included 14 species with three outgroups and 34 morphological characters. The monophyly of the group is supported by three sinapomorphies with 100% consistency and retention indices: prozona larger than the metazona, posterior border of the pronotum not angulated and supranal plate shorter than cerci. The analysis resulted in a single cladogram: (P. olivaceus (E. vittatus (L. dorsualis; L. punctifrons) (R. balzapambae (A. trinitatis (S. nigritus; C. bivittatus) (S. volxemi (P. vulneratus; P. sexnotata)))))). Biogeographic comments are presented and related with pre-established standards.

Uma análise cladística do relacionamento entre as espécies sul-americanas do grupo Sitalces (Sitalces Stål, 1878, Eusitalces Bruner, 1911, Parasitalces Bruner, 1911, Psilocirtus Bruner, 1911, Liebermannacris Costa & Carvalho, 2006, Robustusacris Costa & Carvalho, 2006, Arimacris Costa & Carvalho, 2006, Salvadoracris Costa & Carvalho, 2006 e Caruaruacris Costa & Carvalho, 2006) é apresentada. A análise incluiu 14 espécies com três grupos-externos e 34 caracteres morfológicos. A monofilia do grupo é suportada por três sinapomorfias com índices de consistência e retenção de 100%: prozona maior que a metazona, borda posterior do pronoto não angulada e placa supranal mais curta em relação aos cercos. A análise resultou em um único cladograma: (P. olivaceus (E. vittatus (L. dorsualis; L. punctifrons) (R. balzapambae (A. trinitatis (S. nigritus; C. bivittatus) (S. volxemi (P. vulneratus; P. sexnotata)))))). Comentários biogeográficos são apresentados e relacionados com padrões pré-estabelecidos.

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The sporulation of the fungi Metarhizium anisopliae var. acridum and Beauveria bassiana in cadavers of the grasshopper Rhammatocerus schistocercoides was studied in dry and humid environments. Both fungi were equally virulent against R. schistocercoides. However, internally, M. anisopliae produced more conidia than B. bassiana at 53% and 75% relative humidity. Externally, there was no sporulation at 53% and 75% RH, and M. anisopliae produced more conidia than B. bassiana at 100% RH.

A esporulação dos fungos Metarhizium anisopliae var. acridum e Beauveria bassiana em cadáveres do gafanhoto Rhammatocerus schistocercoides foi estudada em ambientes seco e úmido. As duas espécies de patógenos foram igualmente patogênicas a R. schistocercoides. Entretanto, internamente, M. anisopliae produziu mais conídios que B. bassiana a 53% e 75% de umidade relativa. Externamente, não houve esporulação a 53% e 75% de umidade relativa e M. anisopliae produziu mais conídios que B. bassiana a 100%.