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Β-lactamases-producing Escherichia coli are a widely distributed source of antimicrobial resistance (AMR), for animals and humans. Little is known about the sensitivity profile and genetic characteristics of E. coli strains isolated from domestic cats. We report a cross-sectional study that evaluated E. coli strains isolated from domestic cats in Panama. For this study the following antibiotics were analyzed: ampicillin, amoxicillin-clavulanate cefepime, cefotaxime, cefoxitin, ceftazidime, aztreonam, imipenem, gentamicin, kanamycin, streptomycin, tetracycline, ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, and chloramphenicol. The data obtained were classified as resistant, intermediate, or sensitive. MDR strains were established when the strain presented resistance to at least one antibiotic from three or more antimicrobial classes. Forty-eight E. coli isolates were obtained, of which 80% presented resistance to at least one of the antibiotics analyzed, while only 20% were sensitive to all (p = 0.0001). The most common resistance was to gentamicin (58%). Twenty-nine percent were identified as multidrug-resistant isolates and 4% with extended spectrum beta-lactamase phenotype. The genes blaTEM (39%), blaMOX(16%), blaACC (16%) and blaEBC (8%) were detected. Plasmid-mediated resistance qnrB (25%) and qnrA (13%) are reported. The most frequent sequence types (STs) being ST399 and we reported 5 new STs. Our results suggest that in intestinal strains of E. coli isolated from domestic cats there is a high frequency of AMR.

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Introduction: The nematode Gurltia paralysans is a neglected angio-neurotropic parasite causing chronic meningomyelitis in domestic cats (Felis catus) as well as wild felids of the genus Leopardus in South America. Adult G. paralysans nematodes parasitize the leptomeningeal veins of the subarachnoid space and/or meningeal veins of the spinal cord parenchyma. The geographic range of G. paralysans encompasses rural and peri-urban regions of Chile, Argentina, Uruguay, Colombia and Brazil. Methods: This case report presents clinical and pathological findings of a G. paralysans-infected cat suffering from severe thrombophlebitis and meningomyelitis resulting in ambulatory paraparesis. Neurological examination of affected cat localized the lesions at the thoracolumbar (T3-L3) and lumbosacral (L4-Cd4) segments. Molecular and morphological characteristics of extracted nematodes from parasitized spinal cord veins confirmed G. paralysans. Additionally, data obtained from a questionnaire answered by cat owners of 12 past feline gurltiosis cases (2014-2015) were here analyzed. Questionnaire collected data on age, gender, geographic location, type of food, hunting behavior, type of prey, and other epidemiological features of G. paralysans-infected cats. Results and Discussion: Data revealed that the majority of cats originated from rural settlements thereby showing outdoor life styles with hunting/predatory behaviors, being in close contact to wild life [i.e. gastropods, amphibians, reptiles, rodents, birds, and wild felids (Leopardus guinia)] and with minimal veterinary assistance. Overall, this neglected angio-neurotropic G. paralysans nematode still represents an important etiology of severe thrombophlebitis and meningomyelitis of domestic cats living in endemic rural areas with high biodiversity of definitive hosts (DH), intermediary (IH), and paratenic hosts (PH). The intention of this study is to generate awareness among veterinary surgeons as well as biologists on this neglected feline neuroparasitosis not only affecting domestic cats but also endangered wild felid species of the genus Leopardus within the South American continent.

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Leptospirosis is a bacterial zoonosis that affects both humans and animals worldwide. Currently, it is known that cats may be susceptible to infection. This study aims to investigate the presence of anti-Leptospira spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-Leptospira spp. antibodies, while Leptospira spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecular diagnostic results and the assessed variables, including clinical data and laboratory results of cats testing positive. This study provides insight into the occurrence of Leptospira spp. infection and leptospiruria in cats treated at a veterinary teaching hospital in southern Brazil.

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The aim of this study was to detect molecularly vector borne pathogens (VBPs) in domiciled cats tested for Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV). Blood samples (n = 119) were analyzed microscopically and molecularly through PCR and sequenced for the detection of the following pathogens: piroplasmids., Bartonella henselae, Cytauxzoon felis, Ehrlichia canis, Leishmania spp., hemotropic Mycoplasma spp., Trypanosoma spp., and Ricketssia spp. Animals were also serological assessed for detection of antibodies against FIV and FeLV. Out of all animals, 20.16% (24/119) tested positive for at least one VBPs at molecular examination. Conversely, no animal resulted positive at microscopic analysis. The most prevalent pathogen was hemotropic Mycoplasma haemofelis (8.40%; 10/119), followed by Candidatus Mycoplasma haemominutum (5.88%; 7/119), E. canis (5.04%; 6/119), C. felis (0.84%; 1/119) and B. henselae (0.84%; 1/119). One animal (0.84%; 1/119) was co-infected with. E. canis and B. henselae. A total of 5.88% (7/119) and 1.68% (2/119) tested positive for FIV and FeLV, respectively. Data of this study demonstrate that owned cats can be at risk of hemotropic Mycoplasma spp., E. canis, C. felis and B. henselae. Therefore, preventive measures against vectors of these pathogens should be implemented in order to reduce the risk of exposition and consequently infection. Additionally, aggressive behaviors among cats should be avoided, especially because hemotropic Mycoplasma spp. may be transmitted through the bite of animals.

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Parasites of the genus Sarcocystis can infect several species of animals and cause multiple diseases such as equine protozoal myeloencephalitis. Felines are considered hosts of this protozoa; therefore, the present study aimed to detect anti-Sarcocystis spp.-specific antibodies in domestic cats that were under clinical evaluation, using the indirect immunofluorescence antibody test. Anti-Sarcocystis-specific immunoglobulin Gs were detected in 24 out of 497 (4.82%) cat serum samples. These findings support the fact that natural Sarcocystis infections do occur in cats. Furthermore, it highlights the importance of domestic cats as both intermediate and definitive hosts in the Sarcocystis life cycle, maintaining the parasite and serving as a source of infection for various other animals. To the best of our knowledge, this is the first study to identify antibodies against the genus Sarcocystis in cats from a region in southern Brazil.

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Two cases of lagochilascariasis minor in domestic cats from Costa Rica within a period of two months are recorded for the first time in Central America. Clinical findings included purulent discharge and a tumor in the left ear in one of the cats, whereas the other cat had an ulcerated cervical lesion. Both patients underwent surgical procedures during which nematode worms were collected and analyzed. The collected nematodes were identified using a combination of morphological and molecular assays, which revealed a 99.1% similarity in the cytochrome oxidase subunit 1 with L. minor from Mexico. The lack of information on this parasitosis, as well as the enormous harm it does to animal and human hosts, highlights the need for more research and awareness in Costa Rica and Central America. Furthermore, the unexpected occurrence of these instances in the same location emphasizes the imminent zoonotic risk to humans and the active circulation of the parasite.

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O nematoide Lagochilascaris sp. pertencente à família Ascarididae é um parasito cujo o hospedeiro definitivo pode ser o gato. Considerada uma zoonose rara na medicina veterinária, a lagoquilascaríase é responsável por causar lesões luminais e possui um tropismo particular pela região cervical e cabeça. A infecção ocorre através da ingestão da carne de animais silvestres mal cozida pelo homem e através da caça pelos felinos. Este trabalho tem como objetivo realizar um levantamento dos casos de lagoquilascaríase ocorridos em gatos domésticos nas cidades de Bento Gonçalves, Farroupilha e Caxias do Sul, localizadas na região da Serra Gaúcha. Foi realizada uma pesquisa através de questionário encaminhado por e-mail, durante os meses de abril a julho de 2020, a 44 médicos veterinários dos municípios de Bento Gonçalves, Farroupilha e Caxias do Sul, sendo 13 (29,54%) veterinários de Bento Gonçalves, 10 (22,73%) de Farroupilha e 21 (47,73%) de Caxias do Sul. Destes profissionais, 17 (38,63%) já atenderam casos de animais parasitados por este nematódeo; 9 (20,45%) em Bento Gonçalves; 3 (6,81%) em Farroupilha e 5 (11,36%) em Caxias do Sul. No total foram atendidos 36 casos de lagoquilascaríase no período de 2016 a 2020, sendo que a maior ocorrência foi na cidade de Bento Gonçalves, com 25 casos, seguida por Caxias do Sul com 6 casos e Farroupilha com apenas 5 casos relatados. Apesar da literatura considerar a lagoquilascaríase uma doença rara, este levantamento mostra que há vários casos acontecendo na espécie felina na Serra Gaúcha.

The nematode Lagochilascaris sp. belonging to the family Ascarididae is a parasite whose definitive host may be the cat. Considered a rare zoonosis in veterinary medicine, lagochilascariasis is responsible for causing luminal lesions and has a particular tropism for the cervical region and head. Infection occurs through the ingestion of undercooked meat from wild animals by humans and through hunting by felines. This work aims to carry out a survey of cases of lagochilascariasis that occurred in domestic cats in the cities of Bento Gonçalves, Farroupilha and Caxias do Sul, located in the Serra Gaúcha region. A survey was carried out through a questionnaire sent by email, during the months of April to July 2020, to 44 veterinarians from the municipalities of Bento Gonçalves, Farroupilha and Caxias do Sul, of which 13 (29.54%) were veterinarians from Bento. Gonçalves, 10 (22.73%) from Farroupilha and 21 (47.73%) from Caxias do Sul. Of these professionals, 17 (38.63%) have already treated cases of animals parasitized by this nematode; 9 (20.45%) in Bento Gonçalves; 3 (6.81%) in Farroupilha and 5 (11.36%) in Caxias do Sul. In total, 36 cases of lagochilascariasis were treated in the period from 2016 to 2020, with the highest occurrence being in the city of Bento Gonçalves, with 25 cases, followed by Caxias do Sul with 6 cases and Farroupilha with only 5 reported cases. Although the literature considers lagochilascariasis a rare disease, this survey shows that there are several cases happening in feline species in Serra Gaúcha.

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Muchas patologías pueden afectar a los felinos, lo que determina de forma rutinaria la necesidad de atención veterinaria. El entorno en el que se inserta el gato es extremadamente importante, y en la mayoría de las clínicas veterinarias y hospitales donde estos animales se mantienen en el hospital, las condiciones no son ideales. El enriquecimiento ambiental es una herramienta que busca mejorar la calidad de vida del animal, posibilitando así la anticipación de la recuperación y consecuentemente un mejor pronóstico para el animal. Con el fin de minimizar un posible nivel de estrés que el entorno hospitalario puede determinar al animal, se realizó un estudio experimental en la Clínica Veterinaria Unigran en Dourados - MS, en el que 3 gatos sanos (2 machos y 1 hembra) con un aproximado Los tres años de edad fueron mantenidos en un ambiente de internación para realizar un estudio de comportamiento de los mismos mediante grabaciones de video y posterior análisis mediante etograma. Los videos se realizaron en dos momentos, el primero fue el cubículo de hospitalización sin enriquecimiento ambiental, mientras que el segundo fue introduciendo elementos (arenero, cama y juguetes) como enriquecimiento. El enriquecimiento ambiental ayudó moderadamente a algunos animales, a reducir el tiempo dedicado a algunas de sus conductas incluidas en la forma de estrés, así como también se observó qu

Many pathologies can affect felines, which routinely determines the need for veterinary care. The environment in which the cat is inserted is extremely important, and in most veterinary clinics and hospitals where these animals are kept in hospital, there are less than ideal conditions. Environmental enrichment is a tool that seeks to improve the quality of life of the animal, thus enabling the anticipation of recovery and consequently better prognosis for the animal. In order to minimize a possible level of stress that the hospital environment can determine to the animal, an experimental study was carried out at the Unigran Veterinary Clinic in Dourados - MS, in which 3 healthy cats (2 males and 1 female) with an approximate age of three years they were kept in an inpatient environment to conduct a behavioral study of them through video recordings and subsequent analysis using ethogram. The videos were made in two moments, the first being the hospitalization cubicle without environmental enrichment, while the second was introducing items (sandbox, bed and toys) as enrichment. Environmental enrichment helped moderately some animals, in reducing the time spent on some of their behaviors included in the form of stress, as well as it was observed that animals in general at the time when the management of environmental enrichment was carried out had greater interaction with the hum

Muitas patologias podem acometer felinos, que corriqueiramente determina a necessidade de atendimento veterinário. O ambiente no qual o gato está inserido adquire extrema importância, sendo que na maioria das clínicas e hospitais veterinários em que estes animais são mantidos em internação há condições aquém das ideais. O enriquecimento ambiental, constitui uma ferramenta que busca melhorar a qualidade de vida do animal, possibilitando assim a antecipação da recuperação e consequentemente melhores prognósticos ao animal. Para minimizar um possível nível de estresse que o ambiente de internação pode determinar ao animal, foi realizado um estudo experimental na Clínica Veterinária da Unigran em Dourados - MS, no qual 3 gatos saudáveis (2 machos e 1 fêmea) com idade aproximada de três anos foram mantidos em ambiente de internação para se realizar estudo comportamental dos mesmos por meio de gravações em vídeos e posterior análise utilizando-se etograma. Os vídeos eram efetuados em dois momentos, sendo no primeiro a baia de internação sem enriquecimento ambiental, enquanto no segundo eram introduzidos ítens (caixa de areia, cama e brinquedos) como enriquecimento. O enriquecimento ambiental ajudou de forma moderada alguns animais, na diminuição do tempo despendidos de alguns de seus comportamentos compreendidos na forma de estresse bem como observou-se que os animais de uma maneira

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Muitas patologias podem acometer felinos, que corriqueiramente determina a necessidade de atendimento veterinário. O ambiente no qual o gato está inserido adquire extrema importância, sendo que na maioria das clínicas e hospitais veterinários em que estes animais são mantidos em internação há condições aquém das ideais. O enriquecimento ambiental, constitui uma ferramenta que busca melhorar a qualidade de vida do animal, possibilitando assim a antecipação da recuperação e consequentemente melhores prognósticos ao animal. Para minimizar um possível nível de estresse que o ambiente de internação pode determinar ao animal, foi realizado um estudo experimental na Clínica Veterinária da Unigran em Dourados - MS, no qual 3 gatos saudáveis (2 machos e 1 fêmea) com idade aproximada de três anos foram mantidos em ambiente de internação para se realizar estudo comportamental dos mesmos por meio de gravações em vídeos e posterior análise utilizando-se etograma. Os vídeos eram efetuados em dois momentos, sendo no primeiro a baia de internação sem enriquecimento ambiental, enquanto no segundo eram introduzidos ítens (caixa de areia, cama e brinquedos) como enriquecimento. O enriquecimento ambiental ajudou de forma moderada alguns animais, na diminuição do tempo despendidos de alguns de seus comportamentos compreendidos na forma de estresse bem como observou-se que os animais de uma maneira geral no momento em que se realizava o manejo de enriquecimento ambiental possuiam maior interação com o ser humano e desta maneira pode-se recomendar o uso de formas que possam tornar o ambiente mais próximo ao encontrado no domicílio do felino.(AU)

Many pathologies can affect felines, which routinely determines the need for veterinary care. The environment in which the cat is inserted is extremely important, and in most veterinary clinics and hospitals where these animals are kept in hospital, there are less than ideal conditions. Environmental enrichment is a tool that seeks to improve the quality of life of the animal, thus enabling the anticipation of recovery and consequently better prognosis for the animal. In order to minimize a possible level of stress that the hospital environment can determine to the animal, an experimental study was carried out at the Unigran Veterinary Clinic in Dourados - MS, in which 3 healthy cats (2 males and 1 female) with an approximate age of three years they were kept in an inpatient environment to conduct a behavioral study of them through video recordings and subsequent analysis using ethogram. The videos were made in two moments, the first being the hospitalization cubicle without environmental enrichment, while the second was introducing items (sandbox, bed and toys) as enrichment. Environmental enrichment helped moderately some animals, in reducing the time spent on some of their behaviors included in the form of stress, as well as it was observed that animals in general at the time when the management of environmental enrichment was carried out had greater interaction with the human being and in this way it can be recommended the use of ways that can make the environment closer to that found in the feline's home.(AU)

Muchas patologías pueden afectar a los felinos, lo que determina de forma rutinaria la necesidad de atención veterinaria. El entorno en el que se inserta el gato es extremadamente importante, y en la mayoría de las clínicas veterinarias y hospitales donde estos animales se mantienen en el hospital, las condiciones no son ideales. El enriquecimiento ambiental es una herramienta que busca mejorar la calidad de vida del animal, posibilitando así la anticipación de la recuperación y consecuentemente un mejor pronóstico para el animal. Con el fin de minimizar un posible nivel de estrés que el entorno hospitalario puede determinar al animal, se realizó un estudio experimental en la Clínica Veterinaria Unigran en Dourados - MS, en el que 3 gatos sanos (2 machos y 1 hembra) con un aproximado Los tres años de edad fueron mantenidos en un ambiente de internación para realizar un estudio de comportamiento de los mismos mediante grabaciones de video y posterior análisis mediante etograma. Los videos se realizaron en dos momentos, el primero fue el cubículo de hospitalización sin enriquecimiento ambiental, mientras que el segundo fue introduciendo elementos (arenero, cama y juguetes) como enriquecimiento. El enriquecimiento ambiental ayudó moderadamente a algunos animales, a reducir el tiempo dedicado a algunas de sus conductas incluidas en la forma de estrés, así como también se observó que los animales en general en el momento en que se realizaba el manejo del enriquecimiento ambiental tenían mayor interacción con el ser humano y de esta forma se puede recomendar que el uso de formas que puedan acercar el entorno al que se encuentra en el hogar del felino.(AU)

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It is estimated that a quarter of all feline neoplasms affect the integumentary system. Cytological examination is of admirable importance for detection of neoplasms in companion animals, being considered a simple and non-invasive method. To date, there are limited information regarding the prevalence of feline skin cancer as well as the use of cytopathology as a diagnostic tool. In this sense, the study aimed to characterize cutaneous and subcutaneous tumors in 192 cats from the archives of a private clinic in São Paulo-SP, Brazil, specialized in the care of felines. Data regarding the animal (breed definition, sex, and age group) and tumor characteristics (biological behavior, cytomorphological category of neoplasm, and cytological suggestion for neoplasm) were obtained in a five-year period. A 95% confidence interval (CI) was estimated to verify the statistical difference between the animal and tumor characteristics. The chi-square test was carried out to verify the association between the tumor biological behavior and animal variables, as well as other tumor characteristics. Most cats were mixed breed and aged 11 to 15 years. Malignant tumors were predominant. A statistical association was observed between cytomorphological category of neoplasm and biological behavior (p<0.001). Mesenchymal (24.5%) and epithelial tumors (25%) presented the highest frequency among those benign and malignant, respectively. The cytological suggestion for neoplasm was also statistically associated with biological behavior (p<0.001). The highest percentage of occurrence of benign and malignant tumors consisted of lipomas (22.9%) and sarcomas (19.3%), respectively.(AU)

Estima-se que um quarto de todas as neoplasias felinas afetam o sistema tegumentar. O exame citológico é impor-tante para detecção de neoplasias em animais de companhia, sendo considerado um método simples e não invasivo. Até o momento, há informações limitadas sobre a prevalência das neoplasias cutâneas felinas, bem como o uso da citopatologia como ferramenta diagnóstica. Nesse sentido, o estudo teve como objetivo caracterizar neoplasias cutâneas e subcutâneas em 192 gatos do arquivo de uma clínica veterinária privada da cidade de São Paulo-SP, Brasil, especializada no atendimento de feli-nos. Dados referentes ao animal (raça, sexo e faixa etária) e características das neoplasias (comportamento biológico, categoria citomorfológica e sugestão citológica) foram obtidos em um período de cinco anos. Foi estimado um intervalo de confiança (IC) de 95% para verificar a diferença estatística entre as características do animal e da neoplasia. O teste do qui-quadrado foi realizado para verificar a associação entre o comportamento biológico da neoplasia e as variáveis dos animais, além de outras características neoplásicas. A maioria dos gatos era sem definição racial e com idade entre 11 e 15 anos. As neoplasias malignas foram predominantes. Foi observada associação estatística entre a categoria citomorfológica e o comportamento biológico da neoplasia (p<0,001). Neoplasias mesenquimais (24,5%) e epiteliais (25%) apresentaram a maior frequência entre os benignos e malignos, respectivamente. A sugestão citológica para neoplasia também foi estatisticamente associada ao comportamento biológico (p<0,001). O maior percentual de ocorrência de tumores benignos e malignos consistiu em lipomas (22,9%) e sar-comas (19,3%), respectivamente.(AU)

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Rabies is an anthropozoonosis that presents approximately 100% lethality and expensive preventive assistance. Many human post-exposure anti-rabies treatments indicated for people at risk of exposure to rabies virus, usually due to some aggression by a mammalian animal, are instituted annually. This study aimed to evaluate the initial care of post-exposure prophylaxis of human rabies in Belo Horizonte between 2007 and 2016, involving accidents with domestic cats. It was found 71.1% agreement between the treatments instituted by health services and the protocol of the Ministry of Health. Treatments classified as insufficient totaled 18.3% and excessive, 10.6%. The animals were healthy in 68% and observable in 68.4% of the cases. Treatments involving the use of anti-rabies vaccine totaled 87.7%. The most frequent treatment adopted by the health services was observing the animals associated with the human anti-rabies vaccination in 56.7% of the cases. Imperfections in the filling of the notifications must be revised to enable improvements for future analyses. As for human prophylactic anti-rabies care, forming a multidisciplinary health team including veterinarians would be well on the way to improving the service offered.(AU)

A raiva é uma antropozoonose que apresenta aproximadamente 100% de letalidade e alto custo na assistência pre-ventiva. É instituído anualmente, um elevado número de tratamento antirrábico humano pós-exposição, o qual é indicado para pessoas que se encontram sob risco de exposição ao vírus rábico, geralmente devido a alguma agressão por animal mamí-fero. Este trabalho teve como objetivo avaliar o atendimento inicial da profilaxia pós-exposição da raiva humana, em Belo Horizonte, entre os anos de 2007 a 2016, envolvendo acidentes com gatos domésticos. Encontrou-se 71,1% de concordân-cia entre os tratamentos instituídos pelos serviços de saúde e o protocolo do Ministério da Saúde. Tratamentos classificados como insuficientes totalizaram 18,3% e excessivos 10,6%. Os animais eram sadios em 68% e observáveis em 68,4% dos casos. Tratamentos que envolviam o uso de vacina antirrábica totalizaram 87,7%. O tratamento adotado pelos serviços de saúde com maior frequência foi a observação do animal associada à vacinação antirrábica humana em 56,7% dos casos. É necessário que imperfeições no preenchimento das notificações sejam revistas de forma a possibilitar melhorias para análises futuras. Quanto ao atendimento antirrábico profilático humano, a formação de uma equipe multidisciplinar de saúde com inclusão de médi-cos veterinários seria uma forma de aprimorar o serviço oferecido.(AU)

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Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in domestic and wild cats. However, little is known about natural viral infections of domestic cats, although their importance for modelling disease spread, informing strategies for managing positive human-animal relationships and disease prevention. Here, we describe the SARS-CoV-2 infection in a household of two human adults and sibling cats (one male and two females) using real-time RT-PCR, an ELISA test, viral sequencing, and virus isolation. On May 5th, 2020, the cat-owners tested positive for SARS-CoV-2. Two days later, the male cat showed mild respiratory symptoms and tested positive. Four days after the male cat, the two female cats became positive, asymptomatically. Also, one human and one cat showed antibodies against SARS-CoV-2. All cats excreted detectable SARS-CoV-2 RNA for a shorter duration than humans and viral sequences analysis confirmed human-to-cat transmission. We could not determine if cat-to-cat transmission also occurred.

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Landscape anthropization has been identified as one of the main drivers of pathogen emergence worldwide, facilitating pathogen spillover between domestic species and wildlife. The present study investigated Carnivore protoparvovirus-1 infection using molecular methods in 98 free-ranging wild guignas (Leopardus guigna) and 262 co-occurring owned, free-roaming rural domestic cats. We also assessed landscape anthropization variables as potential drivers of infection. Protoparvovirus DNA was detected in guignas across their entire distribution range, with observed prevalence of 13.3% (real-time PCR) and 9% (conventional PCR) in guignas, and 6.1% (conventional PCR) in cats. Prevalence in guigna did not vary depending on age, sex, study area or landscape variables. Prevalence was higher in juvenile cats (16.7%) than in adults (4.4%). Molecular characterization of the virus by amplification and sequencing of almost the entire vp2 gene (1,746 bp) from one guigna and five domestic cats was achieved, showing genetic similarities to canine parvovirus 2c (CPV-2c) (one guigna and one cat), feline panleukopenia virus (FPV) (one cat), CPV-2 (no subtype identified) (two cats), CPV-2a (one cat). The CVP-2c-like sequence found in a guigna clustered together with domestic cat and dog CPV-2c sequences from South America, suggesting possible spillover from a domestic to a wild species as the origin of infection in guigna. No clinical signs of disease were found in PCR-positive animals except for a CPV-2c-infected guigna, which had haemorrhagic diarrhoea and died a few days after arrival at a wildlife rescue centre. Our findings reveal widespread presence of Carnivore protoparvovirus-1 across the guigna distribution in Chile and suggest that virus transmission potentially occurs from domestic to wild carnivores, causing severe disease and death in susceptible wild guignas.

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RESUMO

This study aimed to determine the erythrocyte phenotypes of the feline AB system and to check the presence of antigens other than those present in the feline AB system in domestic cats from Ilhéus-Itabuna microregion, Bahia, Brazil. Three-hundred feline blood samples were collected at the Veterinary Hospital of the "Universidade Estadual de Santa Cruz" (UESC) and in home visits to perform blood phenotyping using the tube-method testing. The reverse phenotyping was made between cats that tested phenotype B with blood samples of cats that tested phenotype A to confirm the blood phenotype B. The cross-tested among cats with phenotype A was made in order to verify the presence of different antigens of AB system in this blood phenotype. The results underwent macroscopic and microscopic analyses. Among the 300 animals tested, regarding breed, 290 were mixed-breed cats and among the remaining ten, five were Persians, four Siamese, and one Angora. 297 (99%) presented with phenotype A (including all the breeding cats) and three (1%) with phenotype B, and all this cats were mixed-breed cats. None (0%) of the cats showed the phenotype AB. All phenotype B bloods reacted to reverse phenotyping with phenotype A, confirming the phenotype B of these cats. All phenotype A bloods were compatible among each other, so no further erythrocyte antigens were detected through this test. The mother of one of the phenotype B cats was identified and had phenotype A, demonstrating phenotype A parents with phenotype B offspring. This finding indicates heterozygosis in the studied population. This data enable to conclude that the studied population presented different erythrocyte phenotypes, subsequently highlighting the importance of conducting phenotype analyses in these animals before performing blood transfusion to avoid serious hemolytic complications associated with incompatibility.(AU)

O objetivo deste estudo foi determinar a frequência dos fenótipos eritrocitários do sistema AB felino e verificar a presença de outros antígenos, não pertencentes ao sistema AB felino, em gatos domésticos das cidades de Ilhéus e Itabuna, Bahia, Brasil. Amostras de sangue de 300 gatos foram coletadas no Hospital Veterinário da Universidade Estadual de Santa Cruz (UESC) e em visitas domiciliares para realizar a fenotipagem sanguínea usando o método de tubo. A fenotipagem reversa foi realizada em gatos que testaram o fenótipo B com amostras que testaram o fenótipo A, para confirmação do fenótipo sanguíneo. O teste cruzado foi realizado entre gatos do fenótipo A, para pesquisar a presença de diferentes antígenos do sistema AB dentro desse fenótipo sanguíneo. Os resultados foram submetidos a análises macroscópicas e microscópicas. Dos 300 animais testados, 110 eram machos e 190 fêmeas, e suas idades variaram de cinco meses à 15 anos. Sobre as raças, 290 eram gatos sem raça definida e dos 10 restantes, cinco eram Persas, quatro eram Siameses e um Angorá. 297 (99%) apresentaram fenótipo A (incluindo todos os gatos de raça) e três (1%) tiveram fenótipo B, sendo todos esses gatos sem raça definida. Nenhum (0%) dos gatos apresentou fenótipo AB. Todos os sangues com fenótipo B reagiram na fenotipagem reversa com o fenótipo A, confirmando o fenótipo B desses gatos. Todos os sangues com fenótipo A foram compatíveis entre si, portanto nenhum antígeno eritrocitário adicional foi detectado através deste teste. A genitora de um dos gatos com fenótipo B, foi encontrada e a mesma possuía fenótipo A, demonstrando pais com fenótipo A e cria com fenótipo B. Esse achado indica heterozigose na população estudada. Esses dados levam à conclusão de que diferentes fenótipos eritrocitários estão presentes na população estudada e destacam a importância da realização de testes fenotípicos nesses animais antes dos procedimentos de transfusão, a fim de evitar complicações hemolíticas graves decorrentes do envolvimento de animais incompatíveis.(AU)

Assuntos

RESUMO

This study aimed to determine the erythrocyte phenotypes of the feline AB system and to check the presence of antigens other than those present in the feline AB system in domestic cats from Ilhéus-Itabuna microregion, Bahia, Brazil. Three-hundred feline blood samples were collected at the Veterinary Hospital of the "Universidade Estadual de Santa Cruz" (UESC) and in home visits to perform blood phenotyping using the tube-method testing. The reverse phenotyping was made between cats that tested phenotype B with blood samples of cats that tested phenotype A to confirm the blood phenotype B. The cross-tested among cats with phenotype A was made in order to verify the presence of different antigens of AB system in this blood phenotype. The results underwent macroscopic and microscopic analyses. Among the 300 animals tested, regarding breed, 290 were mixed-breed cats and among the remaining ten, five were Persians, four Siamese, and one Angora. 297 (99%) presented with phenotype A (including all the breeding cats) and three (1%) with phenotype B, and all this cats were mixed-breed cats. None (0%) of the cats showed the phenotype AB. All phenotype B bloods reacted to reverse phenotyping with phenotype A, confirming the phenotype B of these cats. All phenotype A bloods were compatible among each other, so no further erythrocyte antigens were detected through this test. The mother of one of the phenotype B cats was identified and had phenotype A, demonstrating phenotype A parents with phenotype B offspring. This finding indicates heterozygosis in the studied population. This data enable to conclude that the studied population presented different erythrocyte phenotypes, subsequently highlighting the importance of conducting phenotype analyses in these animals before performing blood transfusion to avoid serious hemolytic complications associated with incompatibility.(AU)

O objetivo deste estudo foi determinar a frequência dos fenótipos eritrocitários do sistema AB felino e verificar a presença de outros antígenos, não pertencentes ao sistema AB felino, em gatos domésticos das cidades de Ilhéus e Itabuna, Bahia, Brasil. Amostras de sangue de 300 gatos foram coletadas no Hospital Veterinário da Universidade Estadual de Santa Cruz (UESC) e em visitas domiciliares para realizar a fenotipagem sanguínea usando o método de tubo. A fenotipagem reversa foi realizada em gatos que testaram o fenótipo B com amostras que testaram o fenótipo A, para confirmação do fenótipo sanguíneo. O teste cruzado foi realizado entre gatos do fenótipo A, para pesquisar a presença de diferentes antígenos do sistema AB dentro desse fenótipo sanguíneo. Os resultados foram submetidos a análises macroscópicas e microscópicas. Dos 300 animais testados, 110 eram machos e 190 fêmeas, e suas idades variaram de cinco meses à 15 anos. Sobre as raças, 290 eram gatos sem raça definida e dos 10 restantes, cinco eram Persas, quatro eram Siameses e um Angorá. 297 (99%) apresentaram fenótipo A (incluindo todos os gatos de raça) e três (1%) tiveram fenótipo B, sendo todos esses gatos sem raça definida. Nenhum (0%) dos gatos apresentou fenótipo AB. Todos os sangues com fenótipo B reagiram na fenotipagem reversa com o fenótipo A, confirmando o fenótipo B desses gatos. Todos os sangues com fenótipo A foram compatíveis entre si, portanto nenhum antígeno eritrocitário adicional foi detectado através deste teste. A genitora de um dos gatos com fenótipo B, foi encontrada e a mesma possuía fenótipo A, demonstrando pais com fenótipo A e cria com fenótipo B. Esse achado indica heterozigose na população estudada. Esses dados levam à conclusão de que diferentes fenótipos eritrocitários estão presentes na população estudada e destacam a importância da realização de testes fenotípicos nesses animais antes dos procedimentos de transfusão, a fim de evitar complicações hemolíticas graves decorrentes do envolvimento de animais incompatíveis.(AU)

Assuntos

RESUMO

ABSTRACT: This study aimed to determine the erythrocyte phenotypes of the feline AB system and to check the presence of antigens other than those present in the feline AB system in domestic cats from Ilhéus-Itabuna microregion, Bahia, Brazil. Three-hundred feline blood samples were collected at the Veterinary Hospital of the Universidade Estadual de Santa Cruz (UESC) and in home visits to perform blood phenotyping using the tube-method testing. The reverse phenotyping was made between cats that tested phenotype B with blood samples of cats that tested phenotype A to confirm the blood phenotype B. The cross-tested among cats with phenotype A was made in order to verify the presence of different antigens of AB system in this blood phenotype. The results underwent macroscopic and microscopic analyses. Among the 300 animals tested, regarding breed, 290 were mixed-breed cats and among the remaining ten, five were Persians, four Siamese, and one Angora. 297 (99%) presented with phenotype A (including all the breeding cats) and three (1%) with phenotype B, and all this cats were mixed-breed cats. None (0%) of the cats showed the phenotype AB. All phenotype B bloods reacted to reverse phenotyping with phenotype A, confirming the phenotype B of these cats. All phenotype A bloods were compatible among each other, so no further erythrocyte antigens were detected through this test. The mother of one of the phenotype B cats was identified and had phenotype A, demonstrating phenotype A parents with phenotype B offspring. This finding indicates heterozygosis in the studied population. This data enable to conclude that the studied population presented different erythrocyte phenotypes, subsequently highlighting the importance of conducting phenotype analyses in these animals before performing blood transfusion to avoid serious hemolytic complications associated with incompatibility.

RESUMO: O objetivo deste estudo foi determinar a frequência dos fenótipos eritrocitários do sistema AB felino e verificar a presença de outros antígenos, não pertencentes ao sistema AB felino, em gatos domésticos das cidades de Ilhéus e Itabuna, Bahia, Brasil. Amostras de sangue de 300 gatos foram coletadas no Hospital Veterinário da Universidade Estadual de Santa Cruz (UESC) e em visitas domiciliares para realizar a fenotipagem sanguínea usando o método de tubo. A fenotipagem reversa foi realizada em gatos que testaram o fenótipo B com amostras que testaram o fenótipo A, para confirmação do fenótipo sanguíneo. O teste cruzado foi realizado entre gatos do fenótipo A, para pesquisar a presença de diferentes antígenos do sistema AB dentro desse fenótipo sanguíneo. Os resultados foram submetidos a análises macroscópicas e microscópicas. Dos 300 animais testados, 110 eram machos e 190 fêmeas, e suas idades variaram de cinco meses à 15 anos. Sobre as raças, 290 eram gatos sem raça definida e dos 10 restantes, cinco eram Persas, quatro eram Siameses e um Angorá. 297 (99%) apresentaram fenótipo A (incluindo todos os gatos de raça) e três (1%) tiveram fenótipo B, sendo todos esses gatos sem raça definida. Nenhum (0%) dos gatos apresentou fenótipo AB. Todos os sangues com fenótipo B reagiram na fenotipagem reversa com o fenótipo A, confirmando o fenótipo B desses gatos. Todos os sangues com fenótipo A foram compatíveis entre si, portanto nenhum antígeno eritrocitário adicional foi detectado através deste teste. A genitora de um dos gatos com fenótipo B, foi encontrada e a mesma possuía fenótipo A, demonstrando pais com fenótipo A e cria com fenótipo B. Esse achado indica heterozigose na população estudada. Esses dados levam à conclusão de que diferentes fenótipos eritrocitários estão presentes na população estudada e destacam a importância da realização de testes fenotípicos nesses animais antes dos procedimentos de transfusão, a fim de evitar complicações hemolíticas graves decorrentes do envolvimento de animais incompatíveis.

RESUMO

Resumen Otodectes cynotis es uno de los ácaros responsables de causar dermatosis parasitaria en el pabellón auricular en animales de compañía, lo que les produce prurito intenso e incluso complicaciones óticas por falta del tratamiento adecuado. Su prevalencia en felinos domésticos puede llegar al 37 y el 85 % de los casos de otitis externa. Son variados los productos que pueden utilizarse para el tratamiento de este ácaro, aunque algunos no declaran en su etiquetado su uso en determinadas especies. Por otra parte, los datos disponibles sobre la eficacia comparada de los diversos fármacos utilizados son escasos. Los fármacos más utilizados son selamectina, fipronil, tiabendazol, ivermectina, entre otros. El objetivo de este estudio fue comparar la eficacia acaricida de tres fármacos utilizados en el tratamiento de otoacariasis por Otodectes cynotis, empleando ivermectina 1 %, fipronil 10 % y selamectina 6 % en gatos domésticos de Puerto Montt. Se revisaron 117 gatos domésticos, de los cuales 60 presentaron otocariasis positiva a Otodectes cynotis. Estos 60 pacientes fueron distribuidos en cuatro grupos de 15 individuos cada uno: grupo A, tratados con ivermectina al 1 %, vía ótica; grupo B, tratados con fipronil al 10 %, vía ótica; grupo C, tratados con selamectina al 6 %, vía tópica (spot-on), y grupo D o grupo control, al que se le aplicó un placebo de glicerina, vía ótica. Se realizaron controles a los 7, 14, 21 y 28 días posteriores a la administración del fármaco. El análisis estadístico demostró leves diferencias significativas entre los grupos A, B y C, con el grupo D. Estos resultados sugieren que los tratamientos para la otocariasis provocada por Otodectes cynotis, con ivermectina, fipronil y selamectina en las concentraciones, dosis y vías de administración anteriormente mencionadas, presentan la misma eficacia, pero con diferencias en el valor por tratamiento.

Abstract Otodectes cynotis is one the mite causing parasitic dermatosis in the ear pinna of pets. It produces an intense itching and even some ear complications in the absence of an adequate treatment. The prevalence in domestic cats may reach to 37% and accounts for 85 % of the external otitis cases. Products for treating this mite problem are assorted, even though some of them do not state in the labels whether they are intended to any specific species. On the other hand, there is scarce data available on the compared efficacy of the different drugs used for treatment. Mostly used drugs include selamectin, fipronil, thiabendazole, ivermectin, among others. This study aims to compare the anti-mite efficacy of three drugs in the treatment of otoacariasis caused by Otodectes cynotis, administering ivermectin 1%, fipronil 10%, and selamectin 6% to domestic cats in Puerto Montt. One hundred and seventeen domestic cats were examined and 60 were positive to otoacariasis caused by Otodectes cynotis. These 60 patients were divided into four groups with 15 subjects each: group A, ivermectin 1%, ear drops; group B, fipronil 10%, ear drops; group C, selamectin 6%, topical (spot-on); and group D or control group that received a glycerin placebo as ear drops. Checkups were carried out on days 7, 14, 21 and 28 after applying the drugs. The statistical analysis showed significant mild differences between A, B and C groups and the D group. These results suggest that the treatments with ivermectin, fipronil and selamectin in the concentrations, dosages and administration routes indicated above to solve the otoacariasis caused by Otodectes cynotis are equally efficacious, but show some differences in the specific value of each treatment.

RESUMO

Felid alphaherpesvirus 1 (FeHV-1) and feline calicivirus (FCV) affect cats worldwide. The aim of this study was to evaluate the frequency of occurrence of FeHV-1 and FCV in cats with clinical signs of respiratory, oral and/or ocular disease. Samples were collected from cats cared for in veterinary ambulatory and clinics and submitted to molecular detection and viral isolation. Of the 49 cats evaluated, 45 (92%) were positive for at least one of the viruses; 82% (40/49) were positive for FeHV-1 and 41% (20/49) for FCV. Of these, 31% (15/49) were coinfection cases. For FeHV-1, 45% (18/40) of the cats tested were positive from the collection of eye swab, and the same percentage (9/20) was obtained for the FCV by the oral swab. FeHV-1 and/or FCV were isolated in 35% (17/49) of the samples. The main clinical sign observed was ocular secretion in 71% (35/49) of cats, characterized as mild serous, purulent or serosanguineous, and in some cases associated with ocular injury and marked chemosis. Our findings demonstrate the high occurrence of FeHV-1 and FCV in domestic cats in southern Brazil and indicate that measures should be implemented to improve the diagnostic, prevention and management against of these important diseases.(AU)

Alphaherpesvírus felídeo 1 (FeHV-1) e calicivírus felino (FCV) afetam gatos mundialmente. O objetivo deste estudo foi identificar a frequência de ocorrência de FeHV-1 e FCV em gatos com sinais clínicos de doença respiratória, oral e/ou ocular. Amostras foram coletadas de gatos atendidos em ambulatório e clínicas veterinárias e submetidas à detecção molecular e isolamento viral. Dos 49 gatos avaliados, 45 (92%) foram positivos para ao menos um dos vírus; 82% (40/49) foram positivos para o FeHV-1 e 41% (20/49) para o FCV. Destes, 31% (15/49) foram casos de coinfecção. Para o FeHV-1, 45% (18/40) dos gatos foram positivos na coleta do swab ocular, e o mesmo percentual (9/20) foi obtido para o FCV a partir do swab oral. FeHV-1 e/ou FCV foram isolados em 35% (17/49) das amostras. O principal sinal clínico observado foi secreção ocular em 71% (35/49) dos gatos, caracterizada como serosa, purulenta ou serossanguinolenta e, em alguns casos, associada à lesão e quemose. Nossos resultados demonstram a alta ocorrência de FeHV-1 e FCV em gatos domésticos na região Sul do Brasil e indicam que devem ser implementadas medidas para melhorar o diagnóstico, a prevenção e o manejo contra essas importantes doenças.(AU)

Assuntos

RESUMO

Felid alphaherpesvirus 1 (FeHV-1) and feline calicivirus (FCV) affect cats worldwide. The aim of this study was to evaluate the frequency of occurrence of FeHV-1 and FCV in cats with clinical signs of respiratory, oral and/or ocular disease. Samples were collected from cats cared for in veterinary ambulatory and clinics and submitted to molecular detection and viral isolation. Of the 49 cats evaluated, 45 (92%) were positive for at least one of the viruses; 82% (40/49) were positive for FeHV-1 and 41% (20/49) for FCV. Of these, 31% (15/49) were coinfection cases. For FeHV-1, 45% (18/40) of the cats tested were positive from the collection of eye swab, and the same percentage (9/20) was obtained for the FCV by the oral swab. FeHV-1 and/or FCV were isolated in 35% (17/49) of the samples. The main clinical sign observed was ocular secretion in 71% (35/49) of cats, characterized as mild serous, purulent or serosanguineous, and in some cases associated with ocular injury and marked chemosis. Our findings demonstrate the high occurrence of FeHV-1 and FCV in domestic cats in southern Brazil and indicate that measures should be implemented to improve the diagnostic, prevention and management against of these important diseases.(AU)

Alphaherpesvírus felídeo 1 (FeHV-1) e calicivírus felino (FCV) afetam gatos mundialmente. O objetivo deste estudo foi identificar a frequência de ocorrência de FeHV-1 e FCV em gatos com sinais clínicos de doença respiratória, oral e/ou ocular. Amostras foram coletadas de gatos atendidos em ambulatório e clínicas veterinárias e submetidas à detecção molecular e isolamento viral. Dos 49 gatos avaliados, 45 (92%) foram positivos para ao menos um dos vírus; 82% (40/49) foram positivos para o FeHV-1 e 41% (20/49) para o FCV. Destes, 31% (15/49) foram casos de coinfecção. Para o FeHV-1, 45% (18/40) dos gatos foram positivos na coleta do swab ocular, e o mesmo percentual (9/20) foi obtido para o FCV a partir do swab oral. FeHV-1 e/ou FCV foram isolados em 35% (17/49) das amostras. O principal sinal clínico observado foi secreção ocular em 71% (35/49) dos gatos, caracterizada como serosa, purulenta ou serossanguinolenta e, em alguns casos, associada à lesão e quemose. Nossos resultados demonstram a alta ocorrência de FeHV-1 e FCV em gatos domésticos na região Sul do Brasil e indicam que devem ser implementadas medidas para melhorar o diagnóstico, a prevenção e o manejo contra essas importantes doenças.(AU)

Assuntos