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J Med Virol ; 87(11): 1881-9, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25963945

RESUMO

World group A rotavirus (RVA) surveillance data provides useful estimates of the disease burden, however, indigenous population might require special consideration. The aim of this study was to describe the results of G- and P-types from Brazilian native children ≤ 3 years. Furthermore, selected strains have been analyzed for the VP7, VP6, VP4, and NSP4 encoding genes in order to gain insight into genetic variability of Brazilian strains. A total of 149 samples, collected during 2008-2012, were tested for RVA using ELISA and PAGE, following by RT-PCR and sequencing. RVA infection was detected in 8.7% of samples (13/149). Genotype G2P[4] was detected in 2008 and 2010, G8P[6] in 2009, and G3P[8] in 2011. The phylogenetic analysis of the VP7 and VP4 genes grouped the Brazilian G2P[4] and G3P[8] strains within the lineages currently circulating in humans worldwide. However, the phylogenetic analysis of the VP6 and NSP4 from the Brazilian G2P[4] strains, and the VP7 and NSP4 from the Brazilian G3P[8] strains suggest a distant common ancestor with different animal strains (bovine, caprine, and porcine). The epidemiological and genetic information obtained in the present study is expected to provide an updated understanding of RVA genotypes circulating in the native infant population, and to formulate policies for the use of RVA vaccines in indigenous Brazilian people. Moreover, these results highlight the great diversity of human RVA strains circulating in Brazil, and an in-depth surveillance of human and animal RVA will lead to a better understanding of the complex dynamics of RVA evolution.


Assuntos
Genótipo , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/genética , Brasil , Pré-Escolar , Análise por Conglomerados , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Evolução Molecular , Variação Genética , Humanos , Lactente , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Grupos Populacionais , Rotavirus/química , Rotavirus/isolamento & purificação , Análise de Sequência de DNA , Homologia de Sequência , Proteínas Virais/genética
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