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This study aimed to evaluate the influence of medium voltage electrical stimulation (ES) at three different intensities, 200 V (Treatment 200 V, T200), 300 V (Treatment 200 V, T300), and 400 V (Treatment 400 V, T400) on the initial pH decline in post mortem muscle and the quality parameters on M. longissimus thoracis - Nellore beef, both throughout the ageing process and during frozen storage. The colour, cooking loss, and shear force parameters for samples of aged beef were determined. Additional parameters, like thaw loss, pH, and lipid oxidation were also analyzed for the frozen storage. The shear force and cooking loss decreased and colour parameters increased in Nellore beef ES compared with CON on ageing time (14 days). At frozen storage, quality parameters like pH, a*, and b* were reduced over time, and no negative effect on lipid oxidation was found. Electrical stimulation at 200 V demonstrated effectiveness for decreasing shear force to Nellore beef (M. longissimus thoracis) during frozen storage. The application of medium voltage electrical stimulation can contribute to improved quality and tenderness in Nellore beef, both during ageing and frozen storage conditions.

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Bos taurus indicus temperament is variable and affects beef tenderization. Our objective was to investigate temperament and performance of non­castrated Nellore and identify groups based on Longissimus lumborum (LL) pH decline as well as beef characteristics produced by those groups. We investigated 94 animals with a subset of carcasses (n = 24) selected based on LL pH at 24 h postmortem (pm) to represent two groups: resistant to pH decline (> 5.8 called pH­Res; n = 10) and normal (< 5.7 called pH­Nor; n = 14). Steaks were fabricated from the LL muscle and randomly assigned to aging (2, 7, 14, and 21 days). Sarcomere length, cooking loss, myofibrillar fragmentation index (MFI), and Warner­Bratzler shear force (WBSF) were determined. Data on temperament were investigated in a multivariate approach, while beef data were compared between groups using the analysis of variance. Rectal temperature at the beginning of the finishing phase and total weight gain were greater and related to animals in the pH­Res group (p < 0.01 and p = 0.03, respectively). Temperature and pH decline curves, sarcomere length, and cooking loss revealed that pH­Res produced beef with lower quality compared to the pH­Nor group. Results for MFI and WBSF did not show differences between groups within each time pm; however, overall steaks from pH­Res were tougher (p = 0.06). Incidence of LL pH between 5.8 and 5.9 at 24 h pm did not compromise the tenderization rate or extension; however, it affected the water holding capacity in this population of Nellore cattle.(AU)

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The study investigated meat quality of bulls fed concentrate feeds and hay. The treatments were hay ad libitum + dried cafeteria leftover 4 kg DM d-1(D1); hay ad libitum+ wheat bran 4 kg DM d-1(D2); hay ad libitum+ 4 maize grain 4 kg DM d-1(D3); hay ad libitum+ mix 4 kg DM d-1(1:1, wheat bran to maize grain, respectively (D4)); hay ad libitum+ scrambled whole groundnut 4 kg DM d-1(D5); and hay ad libitum+ mix of each ingredient 4 kg DM d-1(D6)). Samples from longissimus lumborum muscle were taken in triplicate. Beef from bulls fed D5 had highest (p < 0.05) protein and fat than those fed other treatments. However, bulls finished in D3 had similar fat to those fed with whole ground nut. Highest meat tenderness (p < 0.05) recorded at 24thfollowed by 16thd than those aged on other periods. Beef from D6 produced lean meat, which is acceptable to consumer and market demand than D3, produced carcass with highest fat coverage This study confirmed that meat from D6 had an acceptable quality attribute suggesting the breed could serve as a potential source in red meat industry.(AU)

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1. Sausages were used to assess the effects of chicken meat replacement by spent laying hen meat on the quality parameters and sensorial properties. 2. Five formulations were developed: control (CON) containing 100% chicken meat and 4 levels of chicken meat replacement with spent laying hen meat: 25% (T25), 50% (T50), 75% (T75) and 100% (T100). 3. Appearance, texture, succulence, flavour and overall consumer acceptance were analysed. Parameters such as moisture, ash, fat and protein content, pH, cooking loss and colour parameters (lightness (L*), redness (a*), yellowness (b*)) were analysed, as well as the texture properties (hardness, elasticity, cohesiveness and chewiness). 4. Statistical analysis did not reveal significant (P > 0.05) differences in chemical composition and colour parameters among formulations. On the other hand, CON treatment showed the lowest (P < 0.05) cooking loss (20.45%) that increased as spent laying hen meat increased in the formulation (24.92% vs. 27.65% vs. 28.12% vs. 33.05%, for T25, T50, T75 and T100 batches, respectively). 5. Regarding textural parameters, T75 and T100 formulations presented higher (P < 0.05) hardness and chewiness compared to the other ones. 6. Concerning to sensorial characteristics, the T100 formulation presented the lowest average scores (P < 0.05) for all attributes studied. However, the other batches (T25, T50 and T75) did not show significant differences for appearance, texture, succulence, flavour and overall acceptability attributes compared with the CON formulation. 7. The results indicated that the substitution of up to 75% of chicken meat by spent laying hen meat did not decrease the sensory acceptance of the sausages by consumers, demonstrating that this level of substitution in sausages allows better use of spent laying hen meat.

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The aim of the present study was to verify the effect of selenomethionine (SM) supplementation in the diet of chickens on performance, carcass yield, apparent retention, meat quality, and selenium (Se) deposition in tissues. In the first experiment, 2,100 day-old male chicks from the Hubbard Flex strain were randomly distributed in 84 plots with 12 treatments and 7 replicates. The treatments consisted of SM (1,600 ppm) supplementation at levels of 0.3 and 0.5 ppm in substitution of sodium selenite (45.7%) in different preslaughter phases. In the second experiment, 224 day-old male chicks from Hubbard Flex strain were randomly distributed in 28 metabolic cages. Poultry were distributed in 4 treatments with 7 replicates (8 poultry) in the experimental period from 1 to 21 D and experimental plot with 4 poultry aged from 22 to 42 D. Treatments consisted of 4 SM addition levels (0.3, 0.4, 0.5, and 0.6 ppm). In both experiments, the performance (1 to 21 and 1 to 42 D), carcass yield and cuts, apparent retention of Se (33 to 35 D), physical and chemical characteristics of the breast meat were evaluated: objective color, drip loss (DL), cooking loss (CL), pH, peroxide value, and Se deposition in tissues. In experiment I, it was found that SM at 0.3 ppm improved the weight gain and feed conversion of 1 to 42 D. The use of SM at 0.5 ppm resulted in lower DL and CL. The highest Se deposition in muscles was obtained using the SM at 0.5 ppm of 1 to 42 D. Using the SM at 0.5 ppm, only in the last week there was a deposition similar to the use of SM at 0.3 ppm of 1 to 42 D. In experiment II, it can be observed that increased SM levels provided lower DL and lower pH values. Se deposition in tissues of broiler chickens increased linearly at the SM level from 0.3 to 0.6 ppm.

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Abstract The aim of this work was to study the myofibril proteins and collagen fraction changes in broiler chickens PSE (pale, soft, exudative) meat during ageing and their relationship to meat quality. The results presented an increase of myofibril proteins and collagen solubility promoted by the enhanced proteases activities during storage. Ultramicroscopically, the PSE meat samples revealed intracellularly a sarcomere super contraction and lacunas within the A and I bands while Z-lines appeared very dense and fragmented in comparison to normal samples. This observation was noticed already at 4h storage while extracellularly collagen fibrils decreased visually within the endomysium only after 24h of conditioning. These results influenced the quality as the PSE meat presented better functional properties at the first hours of conditioning before further proteins degradation by proteases. Thereafter, at the later ageing stage a further disintegration of the abnormal meat structure would affect the meat functional properties.

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This study was carried out to evaluate the effects of different levels of standardized ileal digestible (SID) methionine + cystine (Met + Cys) and vitamin B6 supplementation on the carcass characteristics and longissimus lumborum (LL) quality in gilts from 75 to 100 kg. Fifty-six gilts were used (Talent x Topigs 20), with an initial average body weight of 75.06 ± 1.68 kg, allotted in a completely randomized block design arranged in a 2×4 factorial scheme, composed of two vitamin B6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met + Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions (P > 0.05) between vitamin B6 supplementation and SID Met + Cys levels were observed. The levels of SID Met + Cys and vitamin B6 supplementation did not affect the carcass characteristics. Thawing loss increased linearly, and a quadratic effect was observed for cooking loss and shear force of the LL when dietary SID Met + Cys levels increased. The highest cooking loss (27.29%) and shear force (21.58 N) were estimated at 0.528 and 0.539% SID Met + Cys levels, respectively. The dietary SID Met + Cys requirement for gilts (75100 kg) did not exceed 10.60 g/day (0.37%), based on carcass characteristics and meat quality parameters, and was not affected by vitamin B6 supplementation.(AU)

O objetivo foi avaliar os efeitos de níveis de metionina+cistina (Met+Cis) digestíveis e da suplementação de vitamina B6 sobre as características de carcaça e qualidade de carne de fêmeas suínas, dos 75 aos 100 kg. Foram utilizados 56 fêmeas suínas (Talent x Topigs 20), com peso médio inicial de 75,06 ± 1,68 kg; distribuídas em um delineamento experimental de blocos casualizados, num esquema fatorial 2×4, constituído de dois níveis suplementares de vitamina B6 (1,58 e 3,58 mg/kg) e quatro níveis de Met+Cis digestíveis (0,370; 0, 470; 0, 570 e 0, 670%), com sete repetições e um animal por unidade experimental. Não foram observadas interações (P > 0,05) entre suplementação de vitamina B6 e os níveis de Met+Cis digestíveis estudados. Os níveis de Met+Cis digestíveis e a suplementação de vitamina B6 não afetaram as características de carcaça. Foi observado um aumento linear na perda de líquido por descongelamento e uma resposta quadrática para a perda de líquido por cocção e força de cisalhamento em função dos níveis de Met+Cis digestíveis. A maior perda por cocção (27,29%) e força de cisalhamento (21,58 N) foram estimados para os níveis de 0,528 e 0,539%, respectivamente. Conclui-se que a exigência de Met+Cis digestível para fêmeas suínas, dos 75 aos 100 kg, é de no máximo 10,60 g/dia (0,370%), com base nas características de carcaça e nos parâmetros de qualidade de carne, não...(AU)

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This study was carried out to evaluate the effects of different levels of standardized ileal digestible (SID) methionine + cystine (Met + Cys) and vitamin B6 supplementation on the carcass characteristics and longissimus lumborum (LL) quality in gilts from 75 to 100 kg. Fifty-six gilts were used (Talent x Topigs 20), with an initial average body weight of 75.06 ± 1.68 kg, allotted in a completely randomized block design arranged in a 2×4 factorial scheme, composed of two vitamin B6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met + Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions (P > 0.05) between vitamin B6 supplementation and SID Met + Cys levels were observed. The levels of SID Met + Cys and vitamin B6 supplementation did not affect the carcass characteristics. Thawing loss increased linearly, and a quadratic effect was observed for cooking loss and shear force of the LL when dietary SID Met + Cys levels increased. The highest cooking loss (27.29%) and shear force (21.58 N) were estimated at 0.528 and 0.539% SID Met + Cys levels, respectively. The dietary SID Met + Cys requirement for gilts (75100 kg) did not exceed 10.60 g/day (0.37%), based on carcass characteristics and meat quality parameters, and was not affected by vitamin B6 supplementation.

O objetivo foi avaliar os efeitos de níveis de metionina+cistina (Met+Cis) digestíveis e da suplementação de vitamina B6 sobre as características de carcaça e qualidade de carne de fêmeas suínas, dos 75 aos 100 kg. Foram utilizados 56 fêmeas suínas (Talent x Topigs 20), com peso médio inicial de 75,06 ± 1,68 kg; distribuídas em um delineamento experimental de blocos casualizados, num esquema fatorial 2×4, constituído de dois níveis suplementares de vitamina B6 (1,58 e 3,58 mg/kg) e quatro níveis de Met+Cis digestíveis (0,370; 0, 470; 0, 570 e 0, 670%), com sete repetições e um animal por unidade experimental. Não foram observadas interações (P > 0,05) entre suplementação de vitamina B6 e os níveis de Met+Cis digestíveis estudados. Os níveis de Met+Cis digestíveis e a suplementação de vitamina B6 não afetaram as características de carcaça. Foi observado um aumento linear na perda de líquido por descongelamento e uma resposta quadrática para a perda de líquido por cocção e força de cisalhamento em função dos níveis de Met+Cis digestíveis. A maior perda por cocção (27,29%) e força de cisalhamento (21,58 N) foram estimados para os níveis de 0,528 e 0,539%, respectivamente. Conclui-se que a exigência de Met+Cis digestível para fêmeas suínas, dos 75 aos 100 kg, é de no máximo 10,60 g/dia (0,370%), com base nas características de carcaça e nos parâmetros de qualidade de carne, não...

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ABSTRACT Recent research on Vitamin K has shown its importance in maintaining vascular and bone health. Brazilian food composition tables do not show phylloquinone content in national foods. These data are needed to obtain more reliable results in nutritional status assessment studies of individuals in relation to this vitamin as studies have shown a geographical influence in food phylloquinone content. This study aims to determine phylloquinone (Vitamin K1) levels in its most important source: dark green leaved vegetables. Several varieties of vegetables were purchased directly from CEAGESP (General Warehouse Company of São Paulo) at different times. Phylloquinone was extracted using organic solvents and quantified by High Performance Liquid Chromatography - HPLC. Results show the concentrations of phylloquinone in commonly consumed foodstuffs. In general, results showed variations with data from literature on the amount of Vitamin K in the plants analysed.

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This study assessed the effects of different levels of oldman saltbush hay and ageing time on the physical characteristics of Santa Inês lamb meat. Sixty shoulders from 32 male lambs fed with 30, 40, 50 or 60% oldman saltbush hay for 60 days were vacuum-packaged and stored in a refrigerator at 0 ± 1°C for 0, 7 or 14 days of ageing. The shear force, cooking loss and water holding capacity were 3.06kgf cm-2, 37.28% and 76.71%, respectively, and there were no significant changed by studied factors (P>0.05).

Objetivou-se com este trabalho avaliar o efeito da inclusão de diferentes níveis de feno de erva-sal e tempo de maturação sobre as características físicas da carne de cordeiros Santa Inês. Sessenta paletas apartir de 32 cordeiros machos, alimentados com 30, 40, 50 e 60% de feno de erva-sal por 60 dias foram embaladas a vácuo e mantidas sob refrigeração a 0±1oC por 0, 7 e 14 dias de maturação. A força de cisalhamento, perda por cocção e capacidade de retenção de água não foram influenciados (P>0,05) pelos fatores estudados, apresentando valores médios de 3,06kgf cm-2, 37,28% e 76,71%, respectivamente.

RESUMO

This study assessed the effects of different levels of oldman saltbush hay and ageing time on the physical characteristics of Santa Inês lamb meat. Sixty shoulders from 32 male lambs fed with 30, 40, 50 or 60% oldman saltbush hay for 60 days were vacuum-packaged and stored in a refrigerator at 0 ± 1°C for 0, 7 or 14 days of ageing. The shear force, cooking loss and water holding capacity were 3.06kgf cm-2, 37.28% and 76.71%, respectively, and there were no significant changed by studied factors (P>0.05).(AU)

Objetivou-se com este trabalho avaliar o efeito da inclusão de diferentes níveis de feno de erva-sal e tempo de maturação sobre as características físicas da carne de cordeiros Santa Inês. Sessenta paletas apartir de 32 cordeiros machos, alimentados com 30, 40, 50 e 60% de feno de erva-sal por 60 dias foram embaladas a vácuo e mantidas sob refrigeração a 0±1oC por 0, 7 e 14 dias de maturação. A força de cisalhamento, perda por cocção e capacidade de retenção de água não foram influenciados (P>0,05) pelos fatores estudados, apresentando valores médios de 3,06kgf cm-2, 37,28% e 76,71%, respectivamente.(AU)

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Estudos sobre a vitamina K têm evidenciado seu papel na manutenção da saúde óssea e vascular. A atuação desta vitamina em tecidos extra-hepáticos não é considerada na recomendação dietética da DRI de 2001, sendo reconhecidas necessidades maiores para função óssea em pessoas acima dos 60 anos e em indivíduos que fazem uso de medicamentos antivitamina K. Visando colaborar com pesquisas futuras, onde o conhecimento do teor de vitamina K1 é relevante, além de contribuir com dados para Tabela Brasileira de Composição de Alimentos - TBCA-USP, o presente trabalho foi conduzido para determinação do teor de filoquinona (vitamina K1) de hortaliças consumidas na cidade de São Paulo. As hortaliças analisadas foram escolhidas após a conclusão de um inquérito alimentar realizado, com adultos e idosos, num projeto de pesquisa desenvolvido junto ao Departamento de Alimentos e Nutrição Experimental da FCF/USP visando a análise da ingestão diária de vitamina K. Desta forma, foram analisadas as hortaliças mais consumidas por esses grupos, submetidas ou não ao processo de cocção. Estas amostras foram adquiridas diretamente na CEAGESP - Companhia de Entrepostos e Armazéns Gerais de São Paulo. As metodologias empregadas na determinação da filoquinona (vitamina K1) foram extração e purificação pelo método líquido-líquido utilizando solventes orgânicos e quantificação através da Cromatografia Líquida de Alta Eficiência (CLAE). Os resultados mostraram que as amostras de hortaliças de colorações verdes mais escuras apresentaram os maiores teores da vitamina como: salsa 491,05 µg/100g; espinafre 375,01 µg/100g, quando comparados com as amostras de colorações verdes mais claras as quais como é o caso da alface americana 113,42 µg/100g e a couve-flor 32,55 µg/100g. A maioria das hortaliças analisadas apresentaram variações entre as diferentes épocas do ano e quando comparadas com dados da literatura

Studies on vitamin K have shown its role in maintaining bone and vascular health. The role of this vitamin in extrahepatic tissues is not considered in the dietary recommendation of DRI 2001. Increased need for vitamin K intake is recognized for the bone function in people over age 60 and individuals who use anti-vitamin K drugs. Aiming at supporting future research, where knowledge of vitamin K1 is relevant, and contribute data to the Brazilian Table of Food Composition - TBCA-USP, this study was conducted to determine the level of phylloquinone (vitamin K1) of vegetables consumed in the city of São Paulo. The vegetables studied were chosen after completion of a nutrition survey carried out with adults and elderly people, a research project developed by the Department of Food and Experimental Nutrition FCF / USP aimed at analyzing the daily intake of vitamin K. Therefore, we analyzed the most consumed vegetables by these groups submitted or not to the cooking process. These samples were acquired directly at CEAGESP - General Warehouses Company of São Paulo. The methodologies employed in the determination of phylloquinone (vitamin K1) were extraction and purification method using liquid-liquid organic solvents followed by High Performance Liquid Chromatography (HPLC). The results showed that the samples of darker green vegetables showed the highest levels of vitamin such as parsley (491.05 µg/100g) and spinach (375.01 µg/100g), when compared with samples of lighter coloration (e.g. 113.42 µg/100g lettuce, and 32.55 µg/100g cauliflower). Most vegetables analyzed showed variation in results when compared to the different seasons and the literature data

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Estudou-se o efeito da recuperação da condição corporal (CC), da estimulação elétrica (EE) e da maturação (M) sobre as características da carcaça e da carne de ovelhas Santa Inês, de descarte por idade, abatidas após o desmame das crias ou após período de recuperação da CC. Avaliaram-se as características da carcaça; a força de cisalhamento (FC); a perda por cocção (PC) e os parâmetros de cor (L*, a* e b*) e efetuou-se a avaliação sensorial da maciez do músculo Longissimus. Concluiu-se que a recuperação da CC melhorou as características da carcaça e clareou a cor da carne, todavia não afetou sua maciez. A EE e a M melhoraram a maciez, reduzindo a FC em 44 a 45% na carne maturada por 7 dias e em 51 a 59% na maturada por 14 dias e entre 32 a 33% na não maturada. A EE clareou a carne, enquanto a M a escureceu, acentuando a coloração vermelha amarronzada e acentuou as perdas por cocção, de 15,2% (sem maturação) a 19,6% e 23,1%, respectivamente, para 7 e 14 dias de M. A avaliação sensorial para maciez corroborou os dados da avaliação instrumental, que indicaram a efetividade da EE na melhoria da maciez da carne.(AU)

Effects of body condition (BC) recovery, carcass electrical stimulation (ES) and meat aging time (AT) were studied on the carcass and loin in Santa Ines ewes culled by age and slaughtered immediately after pup weaning or after BC recovery period. Carcass characteristics, shear force (SF), cooking loss (CL), meat color (L*, a*, b*) and sensory characteristics of Longissimus muscle were evaluated. It was concluded that BC recovery improved carcass characteristics and meat color brightness, however did not affect the tenderness. The ES and AT improved tenderness, reducing SF in 44 - 45% and in 51 - 59%, respectively, for 7 or 14 days of aging and 32 - 33% in non aged meat. ES affected the color parameters, clearing the meat. AT darkened and accentuated the brownish red color of meat and increased the cooking losses, from 15.2% (non aged) to 19.6% and 23.1%, respectively, for 7 and 14 days of AT. The sensory evaluation for tenderness corroborated the findings of the instrumental evaluation, which confirmed the effectiveness of ES in improving meat tenderness.(AU)

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Estudou-se o efeito da recuperação da condição corporal (CC), da estimulação elétrica (EE) e da maturação (M) sobre as características da carcaça e da carne de ovelhas Santa Inês, de descarte por idade, abatidas após o desmame das crias ou após período de recuperação da CC. Avaliaram-se as características da carcaça; a força de cisalhamento (FC); a perda por cocção (PC) e os parâmetros de cor (L*, a* e b*) e efetuou-se a avaliação sensorial da maciez do músculo Longissimus. Concluiu-se que a recuperação da CC melhorou as características da carcaça e clareou a cor da carne, todavia não afetou sua maciez. A EE e a M melhoraram a maciez, reduzindo a FC em 44 a 45% na carne maturada por 7 dias e em 51 a 59% na maturada por 14 dias e entre 32 a 33% na não maturada. A EE clareou a carne, enquanto a M a escureceu, acentuando a coloração vermelha amarronzada e acentuou as perdas por cocção, de 15,2% (sem maturação) a 19,6% e 23,1%, respectivamente, para 7 e 14 dias de M. A avaliação sensorial para maciez corroborou os dados da avaliação instrumental, que indicaram a efetividade da EE na melhoria da maciez da carne.

Effects of body condition (BC) recovery, carcass electrical stimulation (ES) and meat aging time (AT) were studied on the carcass and loin in Santa Ines ewes culled by age and slaughtered immediately after pup weaning or after BC recovery period. Carcass characteristics, shear force (SF), cooking loss (CL), meat color (L*, a*, b*) and sensory characteristics of Longissimus muscle were evaluated. It was concluded that BC recovery improved carcass characteristics and meat color brightness, however did not affect the tenderness. The ES and AT improved tenderness, reducing SF in 44 - 45% and in 51 - 59%, respectively, for 7 or 14 days of aging and 32 - 33% in non aged meat. ES affected the color parameters, clearing the meat. AT darkened and accentuated the brownish red color of meat and increased the cooking losses, from 15.2% (non aged) to 19.6% and 23.1%, respectively, for 7 and 14 days of AT. The sensory evaluation for tenderness corroborated the findings of the instrumental evaluation, which confirmed the effectiveness of ES in improving meat tenderness.

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The glutathione peroxidase (GSH-Px) is selenium-containing enzyme, which catalyses the reduction of hydrogen peroxides and lipid hydroperoxides preventing the oxidative damages in animal tissues. The selenium (Se) deficiency increases the need for vitamin E. The aim of this study was to investigate the effect of dietary supplementation of chelated Se on the poultry meat quality. Fourteen Cobb chicks with 1 day of age were divided into two groups: C-Control group (without supplementation) and S-Supplemented group (with 0.03mg of chelated Se.kg-1 of feed). Birds at 42 days of age were slaughtered and boneless skinless chicken thighs were collected and stored at 18ºC for 30 days. Thighs were analyzed for the cooking loss, lipid oxidation by Thiobarbituric-Acid-Reactive Substances (TBARS) and GSH-Px activity by coupled assay procedure recording the NADPH (nicotinamide adenine dinucleotide phosphate reduced) oxidation. Samples of Supplemented group presented 33.8% less water after cooking (p ? 0.05) and were 23.6% (p ? 0.10) less oxidized when compared with samples of group Control. The GSH-Px activity of thighs did not differ significantly (p> 0.05) between groups Supplemented and Control. The supplementation with 0.03mg of Se in the kg of feed improved the meat quality by reducing the lipid oxidation and cooking loss, however did not increase the GSH-Px activity.

O selênio (Se) é componente da enzima glutationa peroxidase (GSH-Px) que catalisa a redução de peróxido de hidrogênio e peróxido lipídico, prevenindo danos oxidativos nos tecidos animais. A deficiência de Se aumenta a necessidade de vitamina E pelos animais. O objetivo deste trabalho foi avaliar o efeito da suplementação de selênio quelatado sobre a qualidade da carne de frango. Os 14 pintinhos de corte da linhagem Cobb de 1 dia de idade foram divididos em 2 grupos: C-Controle (sem suplementação) e S-Suplementado com 0,03mg Se quelatado. kg-1 de ração. Os frangos com 42 dias de idade foram abatidos e as sobrecoxas desossadas foram coletadas e armazenadas por 30 dias a 18C. As sobrecoxas foram avaliadas quanto a perda por cozimento (PPC), oxidação lipídica pelo método de Substâncias Reativas ao Ácido Tiobarbitúrico (TBARS) e atividade da enzima GSH-Px pela oxidação do NADPH (Nicotinamida Adenina Dinucleotídeo Fosfato Reduzido). As sobrecoxas de frangos do grupo Suplementado perderam 33,8% menos de água após cozimento (p?0,05) e apresentaram-se 23,6% menos oxidadas (p?0,10) quando comparadas com as sobrecoxas do grupo Controle. A atividade da GSH-Px das sobrecoxas não diferiu significativamente (p>0,05) entre os grupos Suplementado e Controle. A suplementação com 0,03mg de selênio quelatado por kg de ração promoveu melhora na qualidade da carne com redução na PPC e na oxidaç

RESUMO

The glutathione peroxidase (GSH-Px) is selenium-containing enzyme, which catalyses the reduction of hydrogen peroxides and lipid hydroperoxides preventing the oxidative damages in animal tissues. The selenium (Se) deficiency increases the need for vitamin E. The aim of this study was to investigate the effect of dietary supplementation of chelated Se on the poultry meat quality. Fourteen Cobb chicks with 1 day of age were divided into two groups: C-Control group (without supplementation) and S-Supplemented group (with 0.03mg of chelated Se.kg-1 of feed). Birds at 42 days of age were slaughtered and boneless skinless chicken thighs were collected and stored at 18ºC for 30 days. Thighs were analyzed for the cooking loss, lipid oxidation by Thiobarbituric-Acid-Reactive Substances (TBARS) and GSH-Px activity by coupled assay procedure recording the NADPH (nicotinamide adenine dinucleotide phosphate reduced) oxidation. Samples of Supplemented group presented 33.8% less water after cooking (p ? 0.05) and were 23.6% (p ? 0.10) less oxidized when compared with samples of group Control. The GSH-Px activity of thighs did not differ significantly (p> 0.05) between groups Supplemented and Control. The supplementation with 0.03mg of Se in the kg of feed improved the meat quality by reducing the lipid oxidation and cooking loss, however did not increase the GSH-Px activity.

O selênio (Se) é componente da enzima glutationa peroxidase (GSH-Px) que catalisa a redução de peróxido de hidrogênio e peróxido lipídico, prevenindo danos oxidativos nos tecidos animais. A deficiência de Se aumenta a necessidade de vitamina E pelos animais. O objetivo deste trabalho foi avaliar o efeito da suplementação de selênio quelatado sobre a qualidade da carne de frango. Os 14 pintinhos de corte da linhagem Cobb de 1 dia de idade foram divididos em 2 grupos: C-Controle (sem suplementação) e S-Suplementado com 0,03mg Se quelatado. kg-1 de ração. Os frangos com 42 dias de idade foram abatidos e as sobrecoxas desossadas foram coletadas e armazenadas por 30 dias a 18C. As sobrecoxas foram avaliadas quanto a perda por cozimento (PPC), oxidação lipídica pelo método de Substâncias Reativas ao Ácido Tiobarbitúrico (TBARS) e atividade da enzima GSH-Px pela oxidação do NADPH (Nicotinamida Adenina Dinucleotídeo Fosfato Reduzido). As sobrecoxas de frangos do grupo Suplementado perderam 33,8% menos de água após cozimento (p?0,05) e apresentaram-se 23,6% menos oxidadas (p?0,10) quando comparadas com as sobrecoxas do grupo Controle. A atividade da GSH-Px das sobrecoxas não diferiu significativamente (p>0,05) entre os grupos Suplementado e Controle. A suplementação com 0,03mg de selênio quelatado por kg de ração promoveu melhora na qualidade da carne com redução na PPC e na oxidaç