RESUMO

Fear conditioning evokes a physiologic release of glucocorticoids that assists learning. As a cochaperone in the glucocorticoid receptor complex, FKBP51 modulates stress-induced glucocorticoid signaling and may influence conditioned fear responses. This study combines molecular and behavioral approaches to examine whether locally reducing FKBP51 expression in the ventral hippocampus is sufficient to affect fear-related behaviors. We hypothesized that reducing FKBP51 expression in the VH would increase glucocorticoid signaling to alter auditory fear conditioning. Adult male rats were injected with an adeno-associated virus (AAV) vector expressing short hairpin - RNAs (shRNA) targeting FKBP5 into the ventral hippocampus to reduce FKBP5 levels or a control AAV. Infusion of FKBP5-shRNA into the ventral hippocampus decreased auditory fear acquisition and recall. Although animals injected with FKBP5-shRNA showed less freezing during extinction recall, the difference was due to a reduced fear recall rather than improved extinction. Reducing ventral hippocampus FKBP51 did not affect exploratory behavior in either the open field test or the elevated zero maze test but did increase passive behavior in the forced swim test, suggesting that the reduction in auditory fear recall was not due to more active responses to acute stress. Furthermore, lower ventral hippocampus FKBP51 levels did not alter corticosterone release in response to restraint stress, suggesting that the reduced fear recall was not due to lower corticosterone release. Our findings suggest FKBP51 in the ventral hippocampus plays a selective role in modulating fear-learning processes and passive behavioral responses to acute stress rather than hypothalamic-pituitary-adrenal axis reactivity or exploratory responses.

Assuntos

Medo , Hipocampo , Proteínas de Ligação a Tacrolimo , Animais , Masculino , Medo/fisiologia , Proteínas de Ligação a Tacrolimo/metabolismo , Proteínas de Ligação a Tacrolimo/genética , Hipocampo/metabolismo , Ratos , Corticosterona/metabolismo , Corticosterona/sangue , Ratos Sprague-Dawley , RNA Interferente Pequeno/metabolismo , RNA Interferente Pequeno/genética , Receptores de Glucocorticoides/metabolismo , Extinção Psicológica/fisiologia

RESUMO

Contextual fear conditioning is a protocol used to assess associative learning across species, including fish. Here, our goal was to expand the analysis of behavioral parameters that may reflect aversive behaviors in a contextual fear conditioning protocol using adult zebrafish (Danio rerio) and to verify how such parameters can be modulated. First, we analyzed the influence of an aversive stimulus (3 mild electric shocks for 5 s each at frequencies of 10, 100 or 1000 Hz) on fish behavior, and their ability to elicit fear responses in the absence of shock during a test session. To confirm whether the aversive responses are context-dependent, behaviors were also measured in a different experimental environment in a test session. Furthermore, we investigated the effects of dizocilpine (MK-801, 2 mg/kg, i.p.) on fear-related responses. Zebrafish showed significant changes in baseline activity immediately after shock exposure in the training session, in which 100 Hz induced robust contextual fear responses during the test session. Importantly, when introduced to a different environment, animals exposed to the aversive stimulus did not show any differences in locomotion and immobility-related parameters. MK-801 administered after the training session reduced fear responses during the test, indicating that glutamate NMDA-receptors play a key role in the consolidation of contextual fear-related memory in zebrafish. In conclusion, by further exploring fear-related behaviors in a contextual fear conditioning task, we show the effects of different shock frequencies and confirm the importance of context on aversive responses for associative learning in zebrafish. Additionally, our data support the use of zebrafish in contextual fear conditioning tasks, as well as for advancing pharmacological studies related to associative learning in translational neurobehavioral research.

Assuntos

Comportamento Animal , Condicionamento Clássico , Maleato de Dizocilpina , Eletrochoque , Antagonistas de Aminoácidos Excitatórios , Medo , Peixe-Zebra , Animais , Medo/efeitos dos fármacos , Medo/fisiologia , Maleato de Dizocilpina/farmacologia , Condicionamento Clássico/efeitos dos fármacos , Condicionamento Clássico/fisiologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Comportamento Animal/efeitos dos fármacos , Masculino , Aprendizagem por Associação/efeitos dos fármacos , Aprendizagem por Associação/fisiologia , Feminino , Locomoção/efeitos dos fármacos

RESUMO

Sepetiba Bay, located in southwestern Rio de Janeiro state, in southeastern Brazil, is a region of extreme anthropogenic impact, and is home to a large population of Guiana dolphins, which face increasing and cumulative pressures on their physical health. Cetacean body condition provides a useful indicator for the evaluation of the conservation status of marine mammals. Given this, the present study quantified the proportion of dolphins with different body condition scores and assessed temporal variation in these scores between 2017 and 2022 through the analysis of photographic records. We analyzed the photographs and identified the individuals using FinFindR and classified each individual based on its apparent body condition. A total of 29,737 photographs were taken during the study, and 79 individuals were identified, of which 68.35% were in poor condition. The evidence suggests that the Guiana dolphins are in relatively poor condition overall, possibly reflecting the cumulative impact of human activities in Sepetiba Bay.

RESUMO

Instrumental appetitive extinction involves the reduction of a previously reinforced response when its occurrence is no longer rewarded. Two experiments with terrestrial toads (Rhinella arenarum) tested whether the occurrence of a nonreinforced response is necessary for response extinction by varying the time of exposure to nonrewarded goal-box stimuli across groups. In Experiment 1, toads that received the same acquisition training (15 sessions, 1 session/day, 300 s of access to water in the goal box) were randomly assigned to two groups. In Group 600 (n=12), animals spent 600 s in the goal box in 8 daily extinction sessions (water present but inaccessible). In Group 0 (n=11), toads performed the runway response (i.e., walking from the start to the goal box) but were removed as soon as they entered the goal box, thus having minimal exposure to nonrewarded goal-box stimuli. The runway response was weakened in Group 600 across extinction trials, but exhibited little change in Group 0. In Experiment 2, toads were randomly assigned to two groups after the same acquisition training. Group 0 (n=7) was treated the same as Group 0 in the previous experiment. In Group RI (retention interval, n=7), toads remained in their home cage for 13 days. Finally, all animals received 4 extinction sessions with 300 s in the empty goal box. There was little behavioral change in Group 0 during the 13 sessions with minimal exposure to the goal box. In extinction, both groups reduced their runway response at similar rates. Although the procedures were instrumental, extinction of the runway response in toads can be accounted for in terms of a Pavlovian approach response to stimuli paired with reward and nonreward in the goal box.

Assuntos

Condicionamento Clássico , Condicionamento Operante , Extinção Psicológica , Animais , Extinção Psicológica/fisiologia , Condicionamento Clássico/fisiologia , Condicionamento Operante/fisiologia , Recompensa , Masculino , Reforço Psicológico , Feminino , Comportamento Animal/fisiologia , Bufonidae/fisiologia , Bufo arenarum/fisiologia

RESUMO

INTRODUCTION: The aim of this study was to evaluate the acute effects of Pilates exercises on flexibility and performance in practitioners of an extreme conditioning program (ECP). The hypothesis adopted was that Pilates could activate deeper muscles and improve the stability of core muscles. METHODS: This was an observational cross-sectional study with individuals practicing an ECP submitted to a Pilates method (PM) exercise session. The Wells bench test was used to assess flexibility, while performance was evaluated using the Wall Ball exercise commonly performed by ECP practitioners. The perception of effort was measured using the Borg scale. RESULTS: A single session of PM exercises was able to significantly improve flexibility immediately after the exercises (p < 0.001) and performance in one of the movements of the ECP (p < 0.001), as well as decrease the perception of effort. CONCLUSION: An acute intervention with Pilates exercises promoted improvement in performance of the CrossFit Wall Ball exercise.

Assuntos

Técnicas de Exercício e de Movimento , Humanos , Técnicas de Exercício e de Movimento/métodos , Estudos Transversais , Masculino , Adulto , Feminino , Adulto Jovem , Amplitude de Movimento Articular/fisiologia , Força Muscular/fisiologia , Músculo Esquelético/fisiologia

RESUMO

The aim of this systematic review was to assess the effects of plyometric-jump training (PJT) on the physical fitness of youth with cerebral palsy (CP) compared with controls (i.e., standard therapy). The PRISMA 2020 guidelines were followed. Eligibility was assessed using the PICOS approach. Literature searches were conducted using the PubMed, Web of Science, and SCOPUS databases. Methodological study quality was assessed using the PEDro scale. Data were meta-analyzed by applying a random-effects model to calculate Hedges' g effect sizes (ES), along with 95% confidence intervals (95% CI). The impact of heterogeneity was assessed (I2 statistic), and the certainty of evidence was determined using the GRADE approach. Eight randomized-controlled studies with low-to-moderate methodological quality were included, involving male (n = 225) and female (n = 138) youth aged 9.5 to 14.6 years. PJT interventions lasted between 8 and 12 weeks with 2-4 weekly sessions. Compared with controls, PJT improved the muscle strength (ES = 0.66 [moderate], 95% CI = 0.36-0.96, p < 0.001, I2 = 5.4%), static (ES = 0.69 [moderate], 95% CI= 0.33-1.04, p < 0.001, I2 = 0.0%) and dynamic balance (ES = 0.85 [moderate], 95% CI = 0.12-1.58, p = 0.023, I2 = 81.6%) of youth with CP. Therefore, PJT improves muscle strength and static and dynamic balance in youth with CP compared with controls. However, more high-quality randomized-controlled trials with larger sample sizes are needed to provide a more definitive recommendation regarding the use and safety of PJT to improve measures of physical fitness.

RESUMO

Social insects live in communities where cooperative actions heavily rely on the individual cognitive abilities of their members. In the honey bee (Apis mellifera), the specialization in nectar or pollen collection is associated with variations in gustatory sensitivity, affecting both associative and non-associative learning. Gustatory sensitivity fluctuates as a function of changes in motivation for the specific floral resource throughout the foraging cycle, yet differences in learning between nectar and pollen foragers at the onset of food collection remain unexplored. Here, we examined nectar and pollen foragers captured upon arrival at food sources. We subjected them to an olfactory proboscis extension reflex (PER) conditioning using a 10% sucrose solution paired (S10%+P) or unpaired (S10%) with pollen as a co-reinforcement. For non-associative learning, we habituated foragers with S10%+P or S10%, followed by dishabituation tests with either a 50% sucrose solution paired (S50%+P) or unpaired (S50%) with pollen. Our results indicate that pollen foragers show lower performance than nectar foragers when conditioned with S10%. Interestingly, performance improves to levels similar to those of nectar foragers when pollen is included as a rewarding stimulus (S10%+P). In non-associative learning, pollen foragers tested with S10%+P displayed a lower degree of habituation than nectar foragers and a higher degree of dishabituation when pollen was used as the dishabituating stimulus (S10%+P). Altogether, our results support the idea that pollen and nectar honey bee foragers differ in their responsiveness to rewards, leading to inter-individual differences in learning that contribute to foraging specialization.

Assuntos

Comportamento Alimentar , Aprendizagem , Néctar de Plantas , Pólen , Recompensa , Animais , Abelhas/fisiologia , Pólen/fisiologia , Comportamento Alimentar/fisiologia , Aprendizagem/fisiologia , Flores/fisiologia , Sacarose/metabolismo

RESUMO

SUMMARY: The purpose of this study was to compare the effects of different training programs based on small-sided games (SSG) and high-intensity interval training (HIIT) on the body composition of female handball players. A total of 45 female handball players (age: 16.13±0.89 years; training experience: 6.18±1.33 years) were assigned to three groups: game group (GG; N = 15), running group (RG; N = 15), and control group (CON; N = 15). The training programs lasted eight weeks, with two training sessions per week. The GG performed three vs. three small-sided games on a 20 m x 20 m court; the RG performed 15 s HIIT running followed by 15 s of active recovery; the CON group maintained regular handball training. Regardless of the group assignment, all participants were measured for each of the following variables: Body height, body mass, fat-free mass, fat mass, muscle mass, intracellular water, and extracellular water. The results demonstrate significant differences (p0.01) in muscle mass. The GG showed significant (p<0.001) greater improvement in these body composition variables than RG and CON. Body composition variables improved with GG, moving from 2.04 % in fat mass to 1.3 % in extracellular water. In the RG, results show significant improvements (p<0.001) only in intra- and extracellular water. The CON shows improvements in fat-free mass, muscle mass, intracellular water, and extracellular water. To conclude, eight weeks of SSG in handball provide better changes in body composition compared to HIIT 15 s - 15 s and handball training alone.

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El propósito de este estudio fue comparar los efectos de diferentes programas de entrenamiento basados en juegos reducidos (SSG) y entrenamiento interválico de alta intensidad (HIIT) sobre la composición corporal de jugadoras de balonmano. Un total de 45 jugadoras de balonmano (edad: 16,13 ± 0,89 años; experiencia de entrenamiento: 6,18 ± 1,33 años) fueron asignadas a tres grupos: grupo de juego (GG; N = 15), grupo de carrera (RG; N = 15) y grupo de control (CON; N = 15). Los programas de formación duraron ocho semanas, con dos sesiones de formación por semana. El GG realizó partidos reducidos de tres contra tres en una cancha de 20 x 20 m; el RG realizó 15 s de carrera HIIT seguidos de 15 s de recuperación activa; El grupo CON mantuvo un entrenamiento regular de balonmano. Independientemente de la asignación de grupo, a todos los participantes se les midió cada una de las siguientes variables: altura corporal, masa corporal, masa libre de grasa, masa grasa, masa muscular, agua intracelular y agua extracelular. Los resultados demuestran diferencias significativas (p0,01) en la masa muscular. El GG mostró una mejora significativa (p<0,001) mayor en estas variables de composición corporal que RG y CON. Las variables de composición corporal mejoraron con GG, pasando de un 2,04 % en masa grasa a un 1,3 % en agua extracelular. En el RG, los resultados mostraron mejoras significativas (p<0,001) sólo en el agua intra y extracelular. El CON indicó mejoras en la masa magra, la masa muscular, el agua intracelular y el agua extracelular. En conclusión, ocho semanas de SSG en balonmano proporcionan mejores cambios en la composición corporal en comparación con el HIIT de 15 s - 15 s y el entrenamiento de balonmano solo.

Assuntos

Humanos , Masculino , Adolescente , Composição Corporal , Exercício Físico , Atletas , Treinamento Intervalado de Alta Intensidade

RESUMO

Objetivo: Analizar los factores condicionantes de las varices en miembros inferiores y el nivel de medidas preventivas en profesionales de enfermería del Hospital San Juan de Dios ­Pisco 2022. Material y métodos: Estudio cuantitativo, descriptivo, no experimental y transversal, con muestra de 92 profesionales de enfermería, con técnica de encuesta y como instrumento un cuestionario validado. Resultados: El 58,7% tuvo regular proporción de factores condicionantes de las várices, 47,8% con alta proporción, y 19,5% con baja proporción, por dimensiones se halló en cuanto a factores no modificables que el 55,4% tenía antecedentes familiares, 68,5% tuvo más de dos embarazos, y 79,3% con labor asistencial mayor de 5 años en factores modificables el 90,2% permanece 5 o más horas de pie, y 55,4% no realiza actividad física al menos 30 minutos diario. Las medidas preventivas fueron regulares en el 69,6%, buenas 20,7% y deficientes en el 9,7%, por dimensiones la alimentación fue mayormente regular 71,7%, actividad física deficiente 67,4%, y cuidados personales regulares 56,5%. Conclusiones: Los factores condicionantes de las várices son de regular proporción en los profesionales de enfermería del Hospital San Juan de Dios de Pisco, y las medidas preventivas son con mayor predominio regulares, existiendo brechas significativas que muestran la necesidad de mejorar las medidas preventivas a un nivel bueno para hacer frente a la gran proporción de factores condicionantes de las várices en este grupo de la población.

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Objective: Analyze the determining factors of varicose veins in the lower limbs and the level of preventive measures in nursing professionals at the San Juan de Dios Hospital -Pisco 2022. Material and methods: Quantitative, descriptive, non-experimental and cross-sectional study, with a sample of 92 nursing professionals, using a survey technique and a validated questionnaire as an instrument. Results: 58.7% had a regular proportion of conditioning factors for varicose veins, 47.8% with a high proportion, and 19.5% with a low proportion, by dimensions it was found in terms of non-modifiable factors that 55.4% had a family history, 68.5% had more after two pregnancies, and 79.3% with care work for more than 5 years, in non-modifiable factors, 90.2% remain 5 or more hours on their feet, and 55.4% do not perform physical activity for at least 30 minutes daily. Preventive measures were regular in 69.6%, good in 20.7% and deficient in 9.7%. By dimensions, food was mostly regular 71.7%, physical activity deficient 67.4%, and regular personal care 56.5%. Conclusions: The conditioning factors of varicose veins are of a regular proportion in nursing professionals at the San Juan de Dios Hospital in Pisco, and preventive measures are more prevalently regular, with significant gaps showing the need to improve preventive measures at a good level to deal with the large proportion of conditioning factors of varicose veins in this population group.

RESUMO

AIM: This study investigated the effectiveness of a drug-modified tissue conditioner in an animal model of denture stomatitis. METHODS AND RESULTS: Wistar rats wore a Candida albicans-contaminated palatal device for 4 days. Next, nystatin (Nys) or chlorhexidine (Chx) were added to a tissue conditioner in their raw or ß-cyclodextrin-complexed (ßCD) forms at their minimum inhibitory concentrations. As controls, one group was not subjected to any procedure (NC), one group used sterile devices, one group had denture stomatitis but was not treated (DS), and another had the devices relined with the tissue conditioner without the addition of any drug (Soft). After 4 days of treatment, treatment effectiveness was assessed visually, histologically, and through CFU count, and myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) assays. Rats from the Soft, Nys, Nys:ßCD, and Chx groups presented a significant decrease in the microbial load compared with the untreated group. Treatment groups showed lower MPO and NAG activity compared to the non-treated group. CONCLUSIONS: The addition of antifungals to a soft tissue conditioner can be a promising approach for denture stomatitis treatment.

Assuntos

Antifúngicos , Candida albicans , Clorexidina , Nistatina , Ratos Wistar , Estomatite sob Prótese , Animais , Estomatite sob Prótese/microbiologia , Estomatite sob Prótese/tratamento farmacológico , Ratos , Antifúngicos/uso terapêutico , Antifúngicos/farmacologia , Nistatina/farmacologia , Nistatina/uso terapêutico , Clorexidina/farmacologia , Candida albicans/efeitos dos fármacos , Modelos Animais de Doenças , Masculino , Contagem de Colônia Microbiana , Testes de Sensibilidade Microbiana , Candidíase Bucal/tratamento farmacológico , Candidíase Bucal/microbiologia , Peroxidase/metabolismo , Acetilglucosaminidase/metabolismo , beta-Ciclodextrinas

RESUMO

Objectives: Primary graft failure (pGF) after hematopoietic stem-cell transplant is associated with considerable morbidity and mortality. The incidence in haplo-HSCT has been reported to be between 0% and 30%. In 2018, we identified a pGF incidence of 35% in our pediatric haplo-HSCT recipients with hematologic malignancies, which motivated us to enact changes to the conditioning regimen.Methods: We performed a single-center prospective, pre-post study of consecutive patients under 16 years with hematologic malignancies, from January 2015 to December 2022 who received a haplo-HSCT. Twenty-six pediatric patients received a haplo-HSCT before September 2018 (G1) and 36 patients after (G2). The main conditioning regimen for G1 was myeloablative with Flu/Cy/Bu, and for G2 the main regimen was reduced intensity Flu/Cy/Mel/TBI2.Results: Nine patients (35%) in G1 had primary graft failure, while in G2 there were no patients with pGF. The median follow-up for G1 was 15.9 months, and for G2 was 24.8 months, with an estimated overall survival at 12 months of 63% (95% CI 47-76) versus 85% (95% CI 73-93), and at 24 months of 47% (95% CI 31-64) versus 70% (95% CI 54-82) respectively (p = .007).Conclusion: After September 2018 conditioning regimen modifications were implemented with the objective of reducing primary failure, consisting mainly of switching from busulfan to melphalan as the alkylating agent of choice, and adding, when clinically possible TBI. Primary failure has been significantly reduced in our institution since then.

Assuntos

Neoplasias Hematológicas , Melfalan , Humanos , Criança , Estudos Prospectivos , Transplante Haploidêntico , Bussulfano

RESUMO

This paper addresses the critical challenge of detecting, separating, and classifying partial discharges in substations. It proposes two solutions: the first involves developing a signal conditioning system to reduce the sampling requirements for PD detection and increase the signal-to-noise ratio. The second approach uses machine learning techniques to separate and classify PD based on features extracted from the conditioned signal. Three clustering algorithms (K-means, Gaussian Mixture Model (GMM), and Mean-shift) and the Support Vector Machine (SVM) method were used for signal separation and classification. The proposed system effectively reduced high-frequency components up to 50 MHz, improved the signal-to-noise ratio, and effectively separated different sources of partial discharges without losing relevant information. An accuracy of up to 93% was achieved in classifying the partial discharge sources. The successful implementation of the signal conditioning system and the machine learning-based signal separation approach opens avenues for more economical, scalable, and reliable PD monitoring systems.

RESUMO

Reduced-intensity conditioning (RIC) regimens have the potential to decrease toxicities related to hematopoietic stem cell transplantation (HCT) in patients with sickle cell disease (SCD). While initial results may have been acceptable in adults and young adults, there are no well-established strategies in children with SCD. Here, it is described the clinical course of two children with symptomatic SCD who have successfully undergone HSCT using Fludarabin-based conditioning.

RESUMO

The enigmatic benefits of acute limb ischemic preconditioning (IP) in enhancing muscle force and exercise performance have intrigued researchers. This study sought to unravel the underlying mechanisms, focusing on increased neural drive and the role of spinal excitability while excluding peripheral factors. Soleus Hoffmann (H)-reflex /M-wave recruitment curves and unpotentiated supramaximal responses were recorded before and after IP or a low-pressure control intervention. Subsequently, the twitch interpolation technique was applied during maximal voluntary contractions to assess conventional parameters of neural output. Following IP, there was an increase in both maximum normalized force and voluntary activation (VA) for the plantar flexor group, with negligible peripheral alterations. Greater benefits were observed in participants with lower VA levels. Despite greater H-reflex gains, soleus volitional (V)-wave and sEMG amplitudes remained unchanged. In conclusion, IP improves muscle force via enhanced neural drive to the muscles. This effect appears associated, at least in part, to reduced presynaptic inhibition and/or increased motoneuron excitability. Furthermore, the magnitude of the benefit is inversely proportional to the skeletal muscle's functional reserve, making it particularly noticeable in under-recruited muscles. These findings have implications for the strategic application of the IP procedure across diverse populations.

Assuntos

Precondicionamento Isquêmico , Músculo Esquelético , Masculino , Humanos , Eletromiografia/métodos , Músculo Esquelético/fisiologia , Contração Muscular/fisiologia , Neurônios Motores/fisiologia , Contração Isométrica/fisiologia , Reflexo H/fisiologia , Estimulação Elétrica

RESUMO

The aim of this study was to verify post-activation performance enhancement (PAPE) in jumping and sprinting after two endurance volume-equated running protocols with different intensities, in runners vs. active individuals. Nine recreational runners (age: 34.5 ± 9.3 years, body mass: 73.1 ± 11.9 kg, body height: 1.76 ± 0.06 m, 17.4 ± 4.4 %body fat; maximum aerobic speed [MAS]: 16.4 ± 1.0 km•h-1), and 9 active individuals (age: 34.1 ± 9.4 years; body mass: 83.2 ± 7.7 kg; body height: 1.79 ± 0.06 m; 25.6 ± 5.4 %body fat; MAS: 13.3 ± 1.2 km•h-1) volunteered for participation. The evaluations were performed over three days as follows: 1) anthropometric measures, physical fitness tests, and the University of Montreal Track Test (UMTT) to determine MAS and the distance to be covered in the running protocols; 2 and 3) the countermovement jump (CMJ) and the flying 20-m sprint (SPRINT) were assessed pre- and post-running at 70% of MAS or a time trial race (TTR), equated by volume and completed in random order. A three-way ANOVA (time*group*running) was performed to analyze the PAPE effects. The results showed a time effect (F = 10 .716; p < 0.01) and a group*running interaction (F = 12.094; p < 0.01) for the CMJ, indicating that active individuals demonstrated PAPE after running at 70% of MAS, while for runners both running interventions (70% of MAS and TTR) induced PAPE in CMJ performances. For the SPRINT, a time*group interaction (F = 4.790; p = 0.044) and a group effect were observed, with runners showing greater SPRINT performances than active individuals. From the current results, it can be suggested that training background and intensity can modulate PAPE responses in jumping and sprinting after volume-equated running protocols at different intensities.

RESUMO

ABSTRACTObjetives: Omega-3 (n3) fatty acids have been studied as an option to alleviate the harmful effects of obesity. However, its role in obesity-related behavioral changes is still controversial. This study aimed to evaluate the effects of n3 on behavior and neuroinflammation in obese animals. Methods: Male Wistar rats were divided into four groups: control diet (CT), CT+n3, cafeteria diet (CAF), and CAF+n3. Diet was administered for 13 weeks, and n3 was supplemented during the last 5 weeks. Metabolic and biochemical parameters were evaluated, as well as anxiety-like behaviors. Immunoblots were conducted in the animals' cerebral cortex and hippocampus to assess changes in neuroinflammatory markers.Results: CAF-fed animals showed higher weight gain, visceral adiposity, fasting glucose, total cholesterol, triglycerides, and insulin levels, and n3 improved the lipid profile and restored insulin sensitivity. CAF-fed rats showed anxiety-like behaviors in the open field and light-dark box tasks but not in the contextual aversive conditioning. Omega-3 did not exert any effect on these behaviors. Regarding neuroinflammation, diet and supplementation acted in a region-specific manner. In the hippocampus, CAF reduced claudin-5 expression with no effect of n3, indicating a brain-blood barrier disruption following CAF. Furthermore, in the hippocampus, the glial fibrillary acidic protein (GFAP) and toll-like receptor 4 (TLR-4) were reduced in treated obese animals. However, n3 could not reverse the TLR-4 expression increase in the cerebral cortex.Discussion: Although n3 may protect against some neuroinflammatory manifestations in the hippocampus, it does not seem sufficient to reverse the increase in anxiolytic manifestations caused by CAF.

Assuntos

Ácidos Graxos Ômega-3 , Receptor 4 Toll-Like , Ratos , Masculino , Animais , Ratos Wistar , Doenças Neuroinflamatórias , Obesidade/etiologia , Obesidade/metabolismo , Dieta , Ácidos Graxos Ômega-3/farmacologia , Ansiedade/etiologia , Ansiedade/prevenção & controle , Suplementos Nutricionais

RESUMO

Few studies have addressed the role of reduced-intensity conditioning (RIC) and non-myeloablative (NMA) regimens in older adults with Philadelphia acute lymphoblastic leukemia (Ph + ALL). The objective of this current study was to compare the outcomes of RIC/NMA versus TBI-based myeloablative (MAC) regimens in Ph + ALL patients older than 40 years old who underwent hematopoietic cell transplantation (HCT) in CR1. We used a freely available database from the CIBMTR. Transplants were performed between 2013 and 2017. With a median follow-up of 37.6 months, we have included 629 patients. We used propensity score weighting. Three-year OSs were 64% in the TBI-MAC group and 66% in the RIC/NMA group. OS was not different (HR = 0.92; p = 0.69). Three-year relapse incidences were 21.6% and 27.6% in the TBI-MAC and RIC/NMA groups. RIC/NMA was not associated with an increase in relapse rate (HR 1.02; p = 0.91). Three-year NRMs were 24.3% in the TBI-MAC group and 20.3% in the RIC/NMA group. RIC/NMA was not associated with superior NRM (HR 0.88; p = 0.57). In summary, we have shown that RIC/NMA regimens achieve outcomes comparable to TBI-based MAC in Ph+ ALL older patients in CR1 who may tolerate a TBI-based MAC regimen.

Assuntos

Transplante de Células-Tronco Hematopoéticas , Leucemia-Linfoma Linfoblástico de Células Precursoras , Adulto , Idoso , Humanos , Doença Aguda , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Recidiva , Condicionamento Pré-Transplante , Pessoa de Meia-Idade , Bases de Dados Factuais

RESUMO

As condições psicopatológicas decorrentes de acontecimentos traumáticos vêm progressivamente recebendo interpretações de natureza neurocientífica. Com isso, experiências humanas devastadoras são reduzidas ao funcionamento perturbado de sistemas neurofisiológicos atribuídos às respostas de estresse. Através de revisão narrativa, este artigo procura explorar algumas das condições epistemológicas elementares ao surgimento de uma teoria neurocientífica do estresse traumático, demonstrando a solidariedade que ela conserva com a teoria evolucionária e com a pesquisa do condicionamento clássico em animais. Espera-se que este trabalho possa salientar algumas das repercussões éticas da negligência dos fatores sociais e culturais nas interpretações dos fenômenos secundários ao traumatismo

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Psychopathological conditions resulting from traumatic events have increasingly received neuroscientific interpretations, reducing the complexity of devastating human experiences to the disturbed functioning of neurophysiological systems attributed to stress responses. This narrative review explores some epistemological conditions essential to fashioning a neuroscientific theory of traumatic stress, showing the solidarity it maintains with evolutionary theory and with research on classical conditioning in animals. We hope this work can highlight some of the ethical repercussions in neglecting social and cultural factors when interpreting secondary trauma phenomena

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Las condiciones psicopatológicas resultantes de eventos traumáticos han recibido progresivamente interpretaciones neurocientíficas. Como resultado, las devastadoras experiencias humanas vieron reducidas su complejidad al funcionamiento perturbado de los sistemas neurofisiológicos atribuidos a las respuestas al estrés. A partir de una revisión narrativa, este artículo buscará explorar algunas de las condiciones epistemológicas esenciales para el surgimiento de una teoría neurocientífica del estrés traumático, demostrando la solidaridad que mantiene con la teoría evolutiva y con la investigación sobre el condicionamiento clásico en animales. Se espera que este trabajo pueda resaltar algunas de las repercusiones éticas de descuidar los factores sociales y culturales en la interpretación de los fenómenos secundarios de trauma

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Les conditions psychopathologiques résultant d'événements traumatiques ont progressivement reçu des interprétations neuroscientifiques, en réduisant la complexité des expériences humaines dévastatrices au fonctionnement perturbé des systèmes neurophysiologiques attribué aux réponses au stress. Cette revue narrative explore quelques conditions épistémologiques essentielles pour élaborer une théorie neuroscientifique du stress traumatique, démontrant la solidarité qu'elle entretient avec la théorie évolutionniste et avec les recherches sur le conditionnement classique. On espère que ce travail pourra mettre en évidence certaines des répercussions éthiques associées à la négligence des facteurs sociaux et culturels dans l'interprétation des phénomènes traumatiques secondaires

Assuntos

Transtornos de Estresse Pós-Traumáticos/etiologia , Condicionamento Clássico , Trauma Psicológico/etiologia , Neurociências

RESUMO

The effect of lactate minimum speed (LMS)-guided training on horses' homeostasis is still unknown. Thus, this study aimed to evaluate the effect of an LMS-guided training program on the fluid, electrolyte and acid-base status of horses. Ten untrained Arabian horses were submitted to an LMS test on a treadmill before and after six weeks of training. The training intensity was 80% of the LMS in the first three sessions and 100% of the LMS in the other sessions. The venous blood was collected before (T-1) and after (T-2) training at rest, during and after the LMS test for lactate, pH, pCO2, HCO3-, and electrolyte measurements. The LMS and strong ion difference (SID4) were calculated. A mild increase in the mean values (p > 0.05) was observed at rest in T-2 in comparison with T-1 in the following variables: pH (from 7.436 ± 0.013 to 7.460 ± 0.012), pCO2 (from 42.95 ± 1.58 to 45.06 ± 0.81 mmHg), HCO3- (from 27.01 ± 1.02 to 28.91 ± 0.86 mmol/L), and SID4 (from 33.42 ± 1.45 to 35.06 ± 2.94 mmol/L). During T-2, these variables were more stable than during T-1. Despite the improvement in fitness, the LMS did not indicate a significant difference (from 5.40 ± 0.55 to 5.52 ± 0.20 m/s). The results confirmed that the LMS-guided training program had a positive impact on the horses' acid-base status, although some adaptations are still required to improve their fitness.