RESUMO

The Bougainvillea glabra or bougainvillea is a climbing plant native from South America belonging to the Nyctaginaceae family. The bougainvillea is recognized worldwide for its horticultural importance, due to the color of its bracts, commonly known as "flowers," made up of bracts, which are the striking parts, and the true flowers, which are white and small. Bougainvillea is widely known in traditional medicine to treat respiratory diseases such as cough, asthma, and bronchitis, gastrointestinal diseases, also for its antibacterial and insecticidal capacity. The antimicrobial potential of the involucre of this plant has not been studied, despite research showing a high phytochemical presence of secondary metabolites such as alkanes, phenols, terpenes, and betalains. This review compiles information about the traditional uses of B. glabra, its botanical description, ecological relevance, phytochemistry, antimicrobial and antibiofilm activity, such as the toxicology of bracts and flowers.

RESUMO

Stenocereus queretaroensis (F.A.C. Weber ex Mathes.) Buxb is a cactus that has long been used as a source food in central and northern México. Its fruits, commonly called pitayas, biosynthesize high amounts of betalains. These molecules are water-soluble nitrogenous compounds; that compared to other pigments, such as anthocyanins or carotenoids, stand out for their physicochemical stability in industrial processes. Due to genetic and environmental factors involved in the biosynthesis and accumulation of secondary metabolites in plants, we tested different stress-inducing agents (elicitor, osmotic, salt, and temperature) to induce betalains accumulation in cell culture from fruits of Stenocereus queretaroensis. This work aimed to understand stress conditions that induce the metabolic pathways required for the accumulation of betalains. The results show how betacyanin concentration increases under high sugar conditions, thus affecting the expression of L-DOPA 4, 5 dioxygenase resulting in a strong dark red coloration. This suggests this enzyme is part of a rate-limiting step in betalain production. In addition, we found that betalains accumulation occurs under particular stress conditions. Cells that have a high level of betacyanins show better resistance to stress in the cell culture, as well as an overall different behavior including cell aggregation and alterations in nuclear size. Together the results shown here may provide new strategies to manipulate and mass produce the pigments from Stenocereus queretaroensis in cell culture.

RESUMO

An improved methodology of achieving an accurate separation of pure fractions of betacyanins and betaxanthins from Ullucus tuberosus Caldas has been carried out. For that purpose, an in-depth chemical identification of each betalain fraction using HPLC-DAD-ESI-MS was developed. This procedure allowed to evaluate the fractionation efficiency and also identify a large number of betalains, most of which have not been described so far in this raw material: betanidin- and isobetanidin-5-O-(4'-O-malonyl-ß-glucoside), 2-decarboxy-phyllocactin, betanidin- and isobetanidin-6-O-(6'-O-feruloyl)-ß-glucoside (gomphrenin and isogomphrenin III), dehydro-phyllocactin and isophyllocactin, and arginine and glycine-betaxanthins (portulacaxanthin III). Moreover, the availability of pure betalain fractions by the proposed methodology permitted to establish the total betalain content and the antioxidant activity of both separated betacyanin and betaxanthin fractions of ulluco for the first time. The results suggest Ullucus tuberosus as an underutilized food bioactive source with a high concentration of total betaxanthins (21.8 µg indicaxanthin/g fresh ulluco) and betacyanins (44.5 µg betanin/g fresh ulluco), strongly correlated to the Folin-Ciocalteau reduction capacity.

Assuntos

RESUMO

Many native species from the Cactaceae family with occurrence in northeastern Brazil have been investigated targeting their chemical composition and functional properties as strategy of adding value to these species that are underexploited according to ethnobotanical studies. The xiquexique (Pilosocereus gounellei (A. Weber ex K. Schum.)) is one of these species that adapts in high temperatures and low rainfall environments and some local populations use it for several purposes, including animal feeding and medicinal. However, there is few studies regarding the physical and physicochemical characterization and on the compounds of functional interest in fruits of this species. In the context, the aim of this study was to evaluate the physical and physicochemical characteristics, bioactive compounds and antioxidant activity of the xiquexique fruit from plants occurring in three municipalities of the state of Paraíba, Brazil. The pulp of fruits from the municipalities of Boqueirão and Solânea presented lower acidity content, with higher SS/AT ratio and may be more suitable for fresh consumption. Furthermore, fruits from these municipalities can be also attractive for processing once they had larger sizes. Fruits of xiquexique exhibited high contents of functional compounds such as phenolic and betalains, which provided high antioxidant activity, with potential for adding value.(AU)

Diversas espécies nativas da família Cactaceae de ocorrência no Nordeste do Brasil têm sido estudadas quanto à qualidade, composição química e propriedades funcionais como estratégia de agregação de valor às essas espécies que são subutilizadas de acordo com estudos etnobotânicos. O xiquexique (Pilosocereus gounellei (A. Weber ex K. Schum.)) é uma dessas espécies que se adapta em ambientes com temperaturas elevadas e baixa pluviosidade e é utilizada pela população local para diversos usos, inclusive na alimentação animal e medicinal. Contudo, há escassez de estudos voltados para a determinação de compostos funcionais nos frutos desta espécie. Neste contexto, o objetivo deste trabalho foi avaliar as características físicas e físico-químicas, os compostos bioativos e a atividade antioxidante de frutos de xiquexique provenientes de três municípios do estado da Paraíba. A polpa dos frutos dos municípios de Boqueirão e Solânea apresentou conteúdo de acidez mais baixo, com relação SS/AT elevada, podendo se adequar para consumo fresco. Além disso, os frutos destes municípios por apresentarem maior tamanho podem também ser atrativos para o processamento. Os frutos de xiquexique apresentam altos conteúdos de compostos funcionais, a exemplo de compostos fenólicos e de betalaínas, o que proporcionou elevada atividade antioxidante, com potencial de agregação de valor.(AU)

Assuntos

RESUMO

Many native species from the Cactaceae family with occurrence in northeastern Brazil have been investigated targeting their chemical composition and functional properties as strategy of adding value to these species that are underexploited according to ethnobotanical studies. The xiquexique (Pilosocereus gounellei (A. Weber ex K. Schum.)) is one of these species that adapts in high temperatures and low rainfall environments and some local populations use it for several purposes, including animal feeding and medicinal. However, there is few studies regarding the physical and physicochemical characterization and on the compounds of functional interest in fruits of this species. In the context, the aim of this study was to evaluate the physical and physicochemical characteristics, bioactive compounds and antioxidant activity of the xiquexique fruit from plants occurring in three municipalities of the state of Paraíba, Brazil. The pulp of fruits from the municipalities of Boqueirão and Solânea presented lower acidity content, with higher SS/AT ratio and may be more suitable for fresh consumption. Furthermore, fruits from these municipalities can be also attractive for processing once they had larger sizes. Fruits of xiquexique exhibited high contents of functional compounds such as phenolic and betalains, which provided high antioxidant activity, with potential for adding value.

Diversas espécies nativas da família Cactaceae de ocorrência no Nordeste do Brasil têm sido estudadas quanto à qualidade, composição química e propriedades funcionais como estratégia de agregação de valor às essas espécies que são subutilizadas de acordo com estudos etnobotânicos. O xiquexique (Pilosocereus gounellei (A. Weber ex K. Schum.)) é uma dessas espécies que se adapta em ambientes com temperaturas elevadas e baixa pluviosidade e é utilizada pela população local para diversos usos, inclusive na alimentação animal e medicinal. Contudo, há escassez de estudos voltados para a determinação de compostos funcionais nos frutos desta espécie. Neste contexto, o objetivo deste trabalho foi avaliar as características físicas e físico-químicas, os compostos bioativos e a atividade antioxidante de frutos de xiquexique provenientes de três municípios do estado da Paraíba. A polpa dos frutos dos municípios de Boqueirão e Solânea apresentou conteúdo de acidez mais baixo, com relação SS/AT elevada, podendo se adequar para consumo fresco. Além disso, os frutos destes municípios por apresentarem maior tamanho podem também ser atrativos para o processamento. Os frutos de xiquexique apresentam altos conteúdos de compostos funcionais, a exemplo de compostos fenólicos e de betalaínas, o que proporcionou elevada atividade antioxidante, com potencial de agregação de valor.

Assuntos

Assuntos

RESUMO

Betalain encapsulation was performed by ionic gelation as a stabilization strategy for these natural pigments. Betalains were extracted from purple cactus fruits and encapsulated in calcium-alginate and in combination of calcium alginate and bovine serum albumin. Beads were characterised by scanning electron microscopy and thermal analysis using differential scanning calorimetry and thermogravimetry. Moisture sorption isotherms were determined. Bead morphology was affected by matrix composition. Pigments storage stability was evaluated at different equilibrium relative humidity and temperatures. Pigment composition of beads was determined by HPLC-MS-MS and degradation products were also analysed after storage; betalamic acid being the major one. Both types of matrices protected the encapsulated pigments, being their storage stability better at low relative humidity than that of the non-encapsulated control material. Antiradical activities of beads were proportional to remaining betalain contents. At high relative humidity, there was no protection and low storage stability was observed in the samples.

Assuntos

RESUMO

This research investigates effects of salicylic acid (an abiotic elicitor) on the antioxidant activity and betacyan production from leaves of Alternanthera tenella cultured in vitro was evaluated. Plants were grown in a liquid MS medium and vermiculite substrate. After 35 days salicylic acid was added to the medium. Content of betacyanins, total phenols and flavonoids and non-enzymatic antioxidant capacity were determined in leaves of A. tenella after 0, 12, 36 and 48h of treatment. After 36h, concentration of betacyanins and total phenols increased. On the other hand, the increase of the treatment time caused a slight decrease in total flavonoids and reduced the DPPH free radical activity. As result the antioxidant activity of the leaves of A. tenella is promoted by salicylic acid and can be attributed to the increase in betacyanin content, which are compounds with recognized antioxidant action.

Este trabalho investiga o efeito do ácido salicílico (um elicitor abiótico) sobre a atividade antioxidante e produção de betacianinas em folhas de Alternanthera tenella cultivada in vitro. As plantas foram cultivadas em meio MS líquido e vermiculita como substrato. Após 35 dias, o ácido salicílico foi adicionado ao meio. Conteúdo de betacianinas, fenóis totais e flavonoides e a capacidade antioxidante não-enzimática foi determinada em folhas de A. tenella após 0, 12, 36 e 48h de tratamento. Após 36h, a concentração de betacianinas e fenóis totais aumentaram. Em contrapartida, o aumento no tempo de exposição causou uma ligeira diminuição nos teores de flavonoides totais e inibição da atividade do radical livre DPPH. Como resultado, a atividade antioxidante de folhas de A. tenella é promovida pelo ácido salicílico e pode ser atribuída ao aumento do conteúdo de betacianina, os quais são compostos com ação antioxidante reconhecida.

RESUMO

This research investigates effects of salicylic acid (an abiotic elicitor) on the antioxidant activity and betacyan production from leaves of Alternanthera tenella cultured in vitro was evaluated. Plants were grown in a liquid MS medium and vermiculite substrate. After 35 days salicylic acid was added to the medium. Content of betacyanins, total phenols and flavonoids and non-enzymatic antioxidant capacity were determined in leaves of A. tenella after 0, 12, 36 and 48h of treatment. After 36h, concentration of betacyanins and total phenols increased. On the other hand, the increase of the treatment time caused a slight decrease in total flavonoids and reduced the DPPH free radical activity. As result the antioxidant activity of the leaves of A. tenella is promoted by salicylic acid and can be attributed to the increase in betacyanin content, which are compounds with recognized antioxidant action.(AU)

Este trabalho investiga o efeito do ácido salicílico (um elicitor abiótico) sobre a atividade antioxidante e produção de betacianinas em folhas de Alternanthera tenella cultivada in vitro. As plantas foram cultivadas em meio MS líquido e vermiculita como substrato. Após 35 dias, o ácido salicílico foi adicionado ao meio. Conteúdo de betacianinas, fenóis totais e flavonoides e a capacidade antioxidante não-enzimática foi determinada em folhas de A. tenella após 0, 12, 36 e 48h de tratamento. Após 36h, a concentração de betacianinas e fenóis totais aumentaram. Em contrapartida, o aumento no tempo de exposição causou uma ligeira diminuição nos teores de flavonoides totais e inibição da atividade do radical livre DPPH. Como resultado, a atividade antioxidante de folhas de A. tenella é promovida pelo ácido salicílico e pode ser atribuída ao aumento do conteúdo de betacianina, os quais são compostos com ação antioxidante reconhecida.(AU)

Assuntos