RESUMO
OBJECTIVES: The establishment of animal models of xenotransplantation can contribute to the elucidation of the molecular pathogenesis of ameloblastic fibrodentinomas (AFD) and it also provides an opportunity for drug tests. We aimed to evaluate the possibility of AFD tumour growth in a patient-derived xenograft (PDX) model. In addition, we characterized the human tumour and the PDXs. MATERIALS AND METHODS: A sample of a recurrent AFD was obtained and two fragments were contralaterally implanted subcutaneously in an 8-week old female NUDE mouse. After 250 days, the PDXs were removed and submitted to histopathological and molecular analysis. Immunohistochemical reactions for Ki67 and the phosphorylated form of ERK1/2 were carried out in both, PDXs and human tumour, and the presence of BRAFV600E was assessed. RESULTS: From day 135 onwards, the PDXs presented a growth peak and remained stable until day 250. Histopathologically, the PDXs presented the same features of the patient's tumour. Tumour cells exhibited Ki67 and pERK1/2 immunoexpression in the patient's tumour and PDX. The AFD was wild-type for BRAFV600E. CONCLUSION: The PDX model recapitulated well the human tumour after a long implantation time, representing a possible model to study the AFD and other odontogenic tumours pathobiology.
Assuntos
Xenoenxertos , Tumores Odontogênicos , Animais , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Nus , Transplante HeterólogoRESUMO
O adenoma canalicular é uma neoplasia benigna incomum de glândulas salivares, que acomete principalmente as glândulas menores, com acentuada predileção pelo lábio superior. Essa neoplasia é mais prevalente em pacientes do sexo feminino, acima de 50 anos de idade. O objetivo deste trabalho é relatar um caso clínico de adenoma canalicular no lábio superior de uma paciente de 68 anos de idade, bem como rever a literatura pertinente. As características clínicas, hipóteses de diagnóstico, histopatologia, tratamento e prognóstico dessa neoplasia são apresentados e discutidos.
The canalicular adenoma is a rare benign salivary gland tumor that affects mainly the minor glands, with a marked predilection for the upper lip. This tumor is more prevalent in female patients above 50 years old. The objective of this study is to report on a case of canalicular adenoma in the upper lip of a 68 years old female patient and to review the pertinent literature. The clinical features, diagnostic hypotheses, histopathology, treatment and prognosis of this tumor are presented and discussed.