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Polycyclic aromatic hydrocarbons (PAHs) are air pollutants generated mainly by fuel combustion, industry, and other anthropogenic sources. The level of these pollutants can be assessed by employing biomonitors, a cost-effective and less contaminating alternative than conventional methods. In the present study, we aimed to investigate whether spiders inhabiting areas around a major city like Córdoba, Argentina, adsorb and retain PAHs in their exoskeletons. Additionally, we aimed to determine if spiders' life traits influence their capacity to accumulate PAHs and explore potential relationships between PAH levels and the types of roads where they were collected. Specimens of a funnel-shaped web spider (Aglaoctenus lagotis) and an orb-weaver (Metepeira spp.) were collected from roadsides. Roads were classified into four categories based on their traffic intensity. Using high-performance liquid chromatography (HPLC), we identified 15 different PAHs. Both species exhibited varying concentrations of PAHs, although Metepeira spp. showed 15-18 times higher PAH levels compared to A. lagotis. Moreover, A. lagotis individuals living alongside highways accumulated up to six times more PAHs compared to those along other road types. These findings suggest that spiders' life traits may influence pollutant concentrations. Our study demonstrates that spiders near roads are exposed to and accumulate PAHs on their exoskeletons, likely sourced from petrogenic vehicular emissions, highlighting their value as biomonitors and emphasizing the need for mitigation measures to address air pollutants emitted from mobile sources.

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Bromeliads play a vital role in preserving biodiversity in the Neotropical region. To understand their impact on arthropod diversity in Brazil's semi-arid region, we studied the rupicolous bromeliad Encholirium spectabile. From 2011 to 2018, we observed the arthropod fauna in E. spectabile clumps, documenting the associated taxa, their abundance, and interactions. We also investigated how seasonality affects arthropod richness and composition during the dry and rainy seasons. Over the observation period, 15 orders and 57 arthropod families were recorded in association with E. spectabile. Insecta dominated, followed by predatory chelicerates. Eight usage categories were identified, with Shelter being the most prevalent, followed by Predators, Nesters, and Nectarivores. Significant differences in taxonomic richness were noted between rainy and dry seasons, with the rainy season exhibiting higher diversity. Seasonal variation was also observed in species composition. Clumps of E. spectabile emerged as crucial habitats for surrounding arthropod fauna. This research underscores the importance of non-phylotelm bromeliads, particularly in high abiotic stress environments like semi-arid regions. The taxonomic diversity observed aligns with findings from diverse environments, shedding light on the relevance of E. spectabile for associated arthropod fauna. These results prompt further exploration of non-phylotelm bromeliads in semi-arid settings, providing a fresh perspective on their significance in shaping arthropod communities.

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Mercury (Hg) is a chemical element that, depending on its concentration, may become toxic to living organisms due to the ability of Hg to bioaccumulate in food chains. In this study, we collected samples of soil, litter, and organisms in the Middle Araguaia floodplain, Brazil. Total mercury (THg) concentrations in litter were significantly higher (p < 0.0001) than that in soil, ranging from 10.68 ± 0.55 to 48.94 ± 0.13 and 20.80 ± 1.07 to 55 .19 ± 1.59 ng g-1, respectively. Total mercury concentration levels in soil showed a linear, inversely proportional relationship with soil organic matter (SOM) contents and soil pH, consistent with the geochemical behavior of chemical elements in flooded environments. Ten orders of organisms were identified, and the average THg concentrations determined in their bodies were up to 20 times higher than those in soil and litter. We found a significant linear relationship between the levels of THg in litter and those found in soil organisms, thereby allowing the prediction of THg concentration levels in soil organisms through the analysis of litter at the sample units. The different dynamics and feeding habits of soil organisms and the concentration of THg in these organisms may be influenced by the river's course. This study provides evidence of the bioaccumulation of THg in soil organisms in the floodplain of the Middle Araguaia River, an important river basin in the Brazilian savanna.

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The protection of soft-skinned fruits against Drosophila suzukii has relied primarily on the efficacy of a few synthetic molecules. Despite their short-term efficacy, these molecules can cause environmental pollution, unintendedly affect non-target organisms, and fail to provide sustainable control. The shortfalls of using synthetic pesticides warrant the search for alternatives, such as essential oils extracted from plants, with greater eco-friendlier properties. Here, we chemically characterized and evaluated the toxicity of the essential oil extracted from leaves of Ocotea indecora (Schott) Mez (Lauraceae) against D. suzukii via two exposure pathways (ingestion and contact). We also assessed the selectivity of the essential oil to two predatory natural enemies, Eriopis connexa and Chrysoperla externa and two pollinator bees, Apis mellifera and Partamona helleri. In addition, we conducted in silico predictions to investigate potential interactions between the major compound of the essential oil and the insects' transient receptor potential (TRP) channels. Our chromatographic analysis revealed sesquirosefuran (87%) as the major compound. Higher toxicity to adults of D. suzukii was observed in contact exposure (LC50 = 0.43 µL mL-1) compared to ingestion (LC50 = 0.72 µL mL-1). However, the essential oil did not cause mortality to the non-target organisms tested here, even when applied at 2.20 µL mL-1. Molecular predictions demonstrated that sesquirosefuran binds more stably to the TRP channels of D. suzukii than to those expressed in beneficial arthropods. Collectively, our findings provide the initial framework for the potential use of O. indecora essential oil as a sustainable alternative for managing D. suzukii infestations.

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Extracellular vesicles (EVs) are lipid-bilayered membrane-delimited particles secreted by almost any cell type, involved in different functions according to the cell of origin and its state. From these, cell to cell communication, pathogen-host interactions and modulation of the immune response have been widely studied. Moreover, these vesicles could be employed for diagnostic and therapeutic purposes, including infections produced by pathogens of diverse types; regarding parasites, the secretion, characterisation, and roles of EVs have been studied in particular cases. Moreover, the heterogeneity of EVs presents challenges at every stage of studies, which motivates research in this area. In this review, we summarise some aspects related to the secretion and roles of EVs from several groups of pathogens, with special focus on the most recent research regarding EVs secreted by extracellular protozoan parasites.

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Abstract Crotalaria (Fabaceae) occurs abundantly in tropical and subtropical regions and has about 600 known species. These plants are widely used in agriculture, mainly as cover plants and green manures, in addition to their use in the management of phytonematodes. A striking feature of these species is the production of pyrrolizidine alkaloids (PAs), secondary allelochemicals involved in plant defense against herbivores. In Crotalaria species, monocrotaline is the predominant PA, which has many biological activities reported, including cytotoxicity, tumorigenicity, hepatotoxicity and neurotoxicity, with a wide range of ecological interactions. Thus, studies have sought to elucidate the effects of this compound to promote an increase in flora and fauna (mainly insects and nematodes) associated with agroecosystems, favoring the natural biological control. This review summarizes information about the monocrotaline, showing such effects in these environments, both above and below ground, and their potential use in pest management programs.

Resumo Crotalaria (Linnaeus, 1753) (Fabaceae) ocorre abundantemente em regiões tropicais e subtropicais e tem cerca de 600 espécies conhecidas. Estas plantas são amplamente utilizadas na agricultura, principalmente como cobertura e adubos verdes, além da sua utilização no manejo de fitonematoides. Uma característica marcante destas espécies é a produção de alcalóides pirrolizidinicos (APs), aleloquímicos secundários envolvidos na defesa das plantas contra os herbívoros. Nas espécies de Crotalaria, a monocrotalina é a AP predominante, que tem muitas atividades biológicas relatadas, incluindo citotoxicidade, tumorigenicidade, hepatotoxicidade e neurotoxicidade, além de uma vasta gama de interações ecológicas. Assim, estudos têm procurado elucidar os efeitos desse composto para promover um incremento na flora e fauna (principalmente insetos e nematoides) associados aos agroecossistemas, favorecendo o controle biológico natural. Esta revisão compila informações sobre a monocrotalina, mostrando tais efeitos nesses ambientes, tanto acima como abaixo do solo e a sua potencial utilização em programas de manejo de pragas.

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Abstract Acacia mangium is a pioneer species with fast growth and frequently used in the recovery of degraded areas. The objectives were to evaluate insects and spiders, their ecological indices and interactions on A. mangium saplings in a tropical degraded area in recovering process. The experimental design was completely randomized with 24 replications, with treatments represented by the first and second years after A. mangium seedling planted. Numbers of leaves/branch, branches/sapling, and ground cover by A. mangium saplings, Hemiptera: Phenacoccus sp. and Pachycoris torridus; Hymenoptera: Tetragonisca angustula and Trigona spinipes, Brachymyrmex sp., Camponotus sp. and Cephalotes sp.; Blattodea: Nasutitermes sp. and Neuroptera: Chrysoperla sp.; abundance, species richness of pollinating insects, tending ants, and the abundance of Sternorrhyncha predators were greatest in the second year after planting. Numbers of Hemiptera: Aethalium reticulatum, Hymenoptera: Camponotus sp., Cephalotes sp., Polybia sp., T. angustula, T. spinipes, tending ants, pollinating insects, Sternorrhyncha predators and species richness of tending ants were highest on A. mangium saplings with greatest numbers of leaves or branches. The increase in the population of arthropods with ground cover by A. mangium saplings age increase indicates the positive impact by this plant on the recovery process of degraded areas.

Resumo Acacia mangium é uma espécie pioneira, de rápido crescimento e utilizada na recuperação de áreas degradadas. Os objetivos foram avaliar insetos e aranhas, seus índices ecológicos e interações com plantas de A. mangium em área tropical degradada em processo de recuperação. O delineamento experimental foi inteiramente casualizado com 24 repetições, com os tratamentos representados pelos primeiro e segundo anos após a plantio de A. mangium. Os números de folhas/galhos, galhos/plantas e cobertura do solo por plantas de A. mangium, de Hemiptera: Phenacoccus sp. e Pachycoris torridus; Hymenoptera: Tetragonisca angustula e Trigona spinipes, Brachymyrmex sp., Camponotus sp. e Cephalotes sp.; Blattodea: Nasutitermes sp. e Neuroptera: Chrysoperla sp.; a abundância, riqueza de espécies de insetos polinizadores, formigas cuidadoras e a abundância de predadores de Sternorrhyncha foram maiores no segundo ano após o plantio. Os números de Hemiptera: Aethalium reticulatum, Hymenoptera: Camponotus sp., Cephalotes sp., Polybia sp., T. angustula, T. spinipes, formigas cuidadoras, insetos polinizadores, predadores de Sternorrhyncha e a riqueza de espécies de formigas cuidadoras foram maiores em plantas de A. mangium com maior altura e número de folhas ou galhos. O aumento populacional de artrópodes e da cobertura do solo com o processo de envelhecimento das plantas de A. mangium indicam impacto positivo dessa planta na recuperação de áreas degradadas.

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Abstract Crotalaria (Fabaceae) occurs abundantly in tropical and subtropical regions and has about 600 known species. These plants are widely used in agriculture, mainly as cover plants and green manures, in addition to their use in the management of phytonematodes. A striking feature of these species is the production of pyrrolizidine alkaloids (PAs), secondary allelochemicals involved in plant defense against herbivores. In Crotalaria species, monocrotaline is the predominant PA, which has many biological activities reported, including cytotoxicity, tumorigenicity, hepatotoxicity and neurotoxicity, with a wide range of ecological interactions. Thus, studies have sought to elucidate the effects of this compound to promote an increase in flora and fauna (mainly insects and nematodes) associated with agroecosystems, favoring the natural biological control. This review summarizes information about the monocrotaline, showing such effects in these environments, both above and below ground, and their potential use in pest management programs.

Resumo Crotalaria (Linnaeus, 1753) (Fabaceae) ocorre abundantemente em regiões tropicais e subtropicais e tem cerca de 600 espécies conhecidas. Estas plantas são amplamente utilizadas na agricultura, principalmente como cobertura e adubos verdes, além da sua utilização no manejo de fitonematoides. Uma característica marcante destas espécies é a produção de alcalóides pirrolizidinicos (APs), aleloquímicos secundários envolvidos na defesa das plantas contra os herbívoros. Nas espécies de Crotalaria, a monocrotalina é a AP predominante, que tem muitas atividades biológicas relatadas, incluindo citotoxicidade, tumorigenicidade, hepatotoxicidade e neurotoxicidade, além de uma vasta gama de interações ecológicas. Assim, estudos têm procurado elucidar os efeitos desse composto para promover um incremento na flora e fauna (principalmente insetos e nematoides) associados aos agroecossistemas, favorecendo o controle biológico natural. Esta revisão compila informações sobre a monocrotalina, mostrando tais efeitos nesses ambientes, tanto acima como abaixo do solo e a sua potencial utilização em programas de manejo de pragas.

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Abstract Acacia mangium is a pioneer species with fast growth and frequently used in the recovery of degraded areas. The objectives were to evaluate insects and spiders, their ecological indices and interactions on A. mangium saplings in a tropical degraded area in recovering process. The experimental design was completely randomized with 24 replications, with treatments represented by the first and second years after A. mangium seedling planted. Numbers of leaves/branch, branches/sapling, and ground cover by A. mangium saplings, Hemiptera: Phenacoccus sp. and Pachycoris torridus; Hymenoptera: Tetragonisca angustula and Trigona spinipes, Brachymyrmex sp., Camponotus sp. and Cephalotes sp.; Blattodea: Nasutitermes sp. and Neuroptera: Chrysoperla sp.; abundance, species richness of pollinating insects, tending ants, and the abundance of Sternorrhyncha predators were greatest in the second year after planting. Numbers of Hemiptera: Aethalium reticulatum, Hymenoptera: Camponotus sp., Cephalotes sp., Polybia sp., T. angustula, T. spinipes, tending ants, pollinating insects, Sternorrhyncha predators and species richness of tending ants were highest on A. mangium saplings with greatest numbers of leaves or branches. The increase in the population of arthropods with ground cover by A. mangium saplings age increase indicates the positive impact by this plant on the recovery process of degraded areas.

Resumo Acacia mangium é uma espécie pioneira, de rápido crescimento e utilizada na recuperação de áreas degradadas. Os objetivos foram avaliar insetos e aranhas, seus índices ecológicos e interações com plantas de A. mangium em área tropical degradada em processo de recuperação. O delineamento experimental foi inteiramente casualizado com 24 repetições, com os tratamentos representados pelos primeiro e segundo anos após a plantio de A. mangium. Os números de folhas/galhos, galhos/plantas e cobertura do solo por plantas de A. mangium, de Hemiptera: Phenacoccus sp. e Pachycoris torridus; Hymenoptera: Tetragonisca angustula e Trigona spinipes, Brachymyrmex sp., Camponotus sp. e Cephalotes sp.; Blattodea: Nasutitermes sp. e Neuroptera: Chrysoperla sp.; a abundância, riqueza de espécies de insetos polinizadores, formigas cuidadoras e a abundância de predadores de Sternorrhyncha foram maiores no segundo ano após o plantio. Os números de Hemiptera: Aethalium reticulatum, Hymenoptera: Camponotus sp., Cephalotes sp., Polybia sp., T. angustula, T. spinipes, formigas cuidadoras, insetos polinizadores, predadores de Sternorrhyncha e a riqueza de espécies de formigas cuidadoras foram maiores em plantas de A. mangium com maior altura e número de folhas ou galhos. O aumento populacional de artrópodes e da cobertura do solo com o processo de envelhecimento das plantas de A. mangium indicam impacto positivo dessa planta na recuperação de áreas degradadas.

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The enzyme glucose-6-phosphate dehydrogenase (G6PDH) plays crucial roles in glucose homeostasis and the pentose phosphate pathway (PPP), being also involved in redox metabolism. The PPP is an important metabolic pathway that produces ribose and nicotinamide adenine dinucleotide phosphate (NADPH), which are essential for several physiologic and biochemical processes, such as the synthesis of fatty acids and nucleic acids. As a rate-limiting step in PPP, G6PDH is a highly conserved enzyme and its deficiency can lead to severe consequences for the organism, in particular for cell growth. Insufficient G6PDH activity can lead to cell growth arrest, impaired embryonic development, as well as a reduction in insulin sensitivity, inflammation, diabetes, and hypertension. While research on G6PDH and PPP has historically focused on mammalian models, particularly human disorders, recent studies have shed light on the regulation of this enzyme in arthropods, where new functions were discovered. This review will discuss the role of arthropod G6PDH in regulating redox homeostasis and immunometabolism and explore potential avenues for further research on this enzyme in various metabolic adaptations.

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BACKGROUND: Rickettsiae are obligate intracellular Gram-negative bacteria that are the causative agent of rickettsioses and are spread to vertebrate hosts by arthropods. There are no previous reports of isolation of Rickettsia amblyommatis for Colombia. METHODS: A convenience sampling was executed in three departments in Colombia for direct collection of adult ticks on domestic animals or over vegetation. Ticks were screened for the presence of Rickettsia spp. by real-time polymerase chain reaction (qPCR) amplifying the citrate synthase gene (gltA), and the positive sample was processed for isolation and further molecular characterization by conventional PCR. The absolute and relative frequencies were calculated for several tick species variables. All products from conventional PCR were further purified and sequenced by the Sanger technique. Representative sequences of 18 Rickettsia species were downloaded from GenBank. Consensus phylogenetic trees were constructed for the gltA, ompB, ompA, and htrA genes with 1000 replicates, calculating bootstrap values through the maximum likelihood method and the generalized time reversible substitution model in the MEGA 7.0 software program. RESULTS: One female Amblyomma mixtum collected on vegetation was amplified by qPCR (gltA), indicating a frequency of 1.6% (1/61) for Rickettsia spp. INFECTION: Sequence analysis of a rickettsial isolate from this tick in BLASTn showed 100% identity with gltA (340 base pairs [bp]), 99.87% for ompB (782 bp), 98.99% for htrA (497 bp), and 100% for ompA (488 bp) to R. amblyommatis. Concatenated phylogenetic analysis confirmed these findings indicating that the isolate is grouped with other sequences of Amblyomma cajennense complex from Panama and Brazil within the R. amblyommatis clade. CONCLUSIONS: This paper describes the isolation and early molecular identification of a R. amblyommatis strain from A. mixtum in Colombia.

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Coendou spinosus is a species of rodent popularly known as porcupine, it has a great ability to adapt to different habitats and is found in tropical forests in countries such as Bolivia, Brazil, Venezuela and Guianas. This mammal has already been identified as a reservoir of several pathogenic agents for humans and other animals and has a variety of ectoparasites, endoparasites and hemoparasites little studied and described. Due to this, the objective was to report the parasitism by Eutrichophilus cercolabes in C. spinosus in the central region of Rio Grande do Sul, southern Brazil. In total, 16 lice were found, one male and 15 females of the species. This is the first report of the parasitism of this Phthiraptera on C. spinosus in Rio Grande do Sul. The scarcity of reports on the taxonomy and biotic characteristics, as well as the vector capacity of pathogens of most species of ectoparasites of wild animals, highlights the need for further studies on the distribution of these arthropods in different regions and host species.

Coendou spinosus é uma espécie de roedor conhecida popularmente por porco-espinho, possui uma grande capacidade de adaptação a diferentes habitats sendo encontrada em florestas tropicais de países como Bolívia, Brasil, Venezuela e Guianas. Esse mamífero já foi identificado como reservatório de vários agentes patogênicos para o homem e outros animais e possui uma variedade de ectoparasitos, endoparasitos e hemoparasitos pouco estudados e descritos. Devido a isso, objetivou-se relatar o parasitismo por Eutrichophilus cercolabes em C. spinosus na região central do Rio Grande do Sul, Sul do Brasil. Ao total, foram encontrados 16 piolhos, sendo um macho e 15 fêmeas da espécie. Esse é o primeiro relato do parasitismo deste Phthiraptera em C. spinosus no Rio Grande do Sul. A escassez de relatos sobre a taxonomia e características bióticas, bem como a capacidade vetorial de patógenos da maioria das espécies de ectoparasitos de animais silvestres evidencia a necessidade da realização de mais estudos sobre a distribuição desses artrópodes em diferentes regiões e espécies hospedeiras.

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The fat body is responsible for a variety of functions related to energy metabolism in arthropods, by controlling the processes of de novo glucose production (gluconeogenesis) and glycogen metabolism. The rate-limiting factor of gluconeogenesis is the enzyme phosphoenolpyruvate carboxykinase (PEPCK), generally considered to be the first committed step in this pathway. Although the study of PEPCK and gluconeogenesis has been for decades restricted to mammalian models, especially focusing on muscle and liver tissue, current research has demonstrated particularities about the regulation of this enzyme in arthropods, and described new functions. This review will focus on arthropod PEPCK, discuss different aspects to PEPCK regulation and function, its general role in the regulation of gluconeogenesis and other pathways. The text also presents our views on potentially important new directions for research involving this enzyme in a variety of metabolic adaptations (e.g. diapause), discussing enzyme isoforms, roles during arthropod embryogenesis, as well as involvement in vector-pathogen interactions, contributing to a better understanding of insect vectors of diseases and their control.

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This work updates the invertebrate type specimen catalog published by Donoso et al. (2009). The catalog is increased by 2281 type specimens (from 454 species or subspecies) to a total of 4180 type specimens (from 770 species or subspecies) hosted at the Pontificia Universidad Católica del Ecuador and Escuela Politécnica Nacional natural history collections. The new material adds 307 holotypes, 1910 paratypes, and 64 allotypes. It provides original information from four phyla (Arthropoda, Mollusca, Nemata, and Platyhelminthes), eight classes, 21 orders, 73 families, and 156 genera. This updated catalog includes a map showing the type localities in the country, a list of the 71 new type specimens (from 23 species or subspecies) from other countries hosted at both museums, corrections to the previous catalog published by Donoso et al. (2009), and label information from each new specimen.

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La obtención de ADN de moscas de interés médico-legal es de relevancia para una variedad de aplicaciones. Aunque existen métodos de extracción comerciales de ADN, su uso rutinario es limitado, en algunos escenarios. En este contexto, el uso de métodos no comerciales constituye una alternativa; sin embargo, su optimización es clave para mejorar el flujo de trabajo y los resultados. Este trabajo evaluó el impacto de variaciones a un método de precipitación salina sobre la concentración y la pureza del ADN recuperado. No se encontraron diferencias significativas en la concentración de ADN extraído entre los diferentes tiempos de incubación, probados durante la fase de extracción, mientras que el incremento en el volumen de etanol absoluto, en la fase de precipitación de ADN, mejoró significativamente la concentración de ADN obtenido. Las modificaciones propuestas reducen el tiempo de ejecución y la concentración de ADN obtenido comparado con el protocolo original.

Obtaining DNA from flies of medico-legal interest is relevant for a variety of applications. Although commercial extraction methods offer optimal DNA, their routine use is limited in some settings. In this context, the use of non-commercial methods constitutes an alternative in laboratories with limited resources however, its optimization is key to improving the workflow and the results. This work evaluated the impact of variations to a saline precipitation method on the concentration and purity of the recovered DNA. No significant differences were found in the concentration of extracted DNA between the different incubation times tested during the extraction phase. In contrast, the increased volume of absolute ethanol in the DNA precipitation phase significantly improved the concentration of DNA obtained. The proposed modifications reduce the runtime and DNA concentration obtained compared with the original protocol.

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Infections caused by multidrug-resistant Acinetobacter baumannii (MDR-Ab) have become a public health emergency. Due to the small therapeutic arsenal available to treat these infections, health agencies have highlighted the importance of developing new antimicrobials against MDR-Ab. In this context, antimicrobial peptides (AMPs) stand out, and animal venoms are a rich source of these compounds. Here, we aimed to summarize the current knowledge on the use of animal venom-derived AMPs in the treatment of MDR-Ab infections in vivo. A systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The eight studies included in this review identified the antibacterial activity of eleven different AMPs against MDR-Ab. Most of the studied AMPs originated from arthropod venoms. In addition, all AMPs are positively charged and rich in lysine residues. In vivo assays showed that the use of these compounds reduces MDR-Ab-induced lethality and bacterial load in invasive (bacteremia and pneumonia) and superficial (wounds) infection models. Moreover, animal venom-derived AMPs have pleiotropic effects, such as pro-healing, anti-inflammatory, and antioxidant activities, that help treat infections. Animal venom-derived AMPs are a potential source of prototype molecules for the development of new therapeutic agents against MDR-Ab.

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The Caribbean enjoys a long-standing eminence as a popular tourist destination; however, over the years it has also amassed the sobriquet "arbovirus hotspot". As the planet warms and vectors expand their habitats, a cognizant working knowledge of the lesser-known arboviruses and the factors that influence their emergence and resurgence becomes essential. The extant literature on Caribbean arboviruses is spread across decades of published literature and is quite often difficult to access, and, in some cases, is obsolete. Here, we look at the lesser-known arboviruses of the insular Caribbean and examine some of the drivers for their emergence and resurgence. We searched the scientific literature databases PubMed and Google Scholar for peer-reviewed literature as well as scholarly reports. We included articles and reports that describe works resulting in serological evidence of the presence of arboviruses and/or arbovirus isolations in the insular Caribbean. Studies without serological evidence and/or arbovirus isolations as well as those including dengue, chikungunya, Zika, and yellow fever were excluded. Of the 545 articles identified, 122 met the inclusion criteria. A total of 42 arboviruses were identified in the literature. These arboviruses and the drivers that affect their emergence/resurgence are discussed.

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A 76-year literature survey and meta-analyses were carried out to recognize the trends, biases, and knowledge gaps of studies focusing on major groups of compounds of botanical origin, or phytochemicals, as insecticides. The survey found that the main phytochemicals prospected as insecticides belong to the following major chemical groups: terpenoids, terpenes, and carbonyl, all of which were tested, mainly against beetles (Coleoptera), caterpillars (i.e., larvae of Lepidoptera), and mosquitoes and other flies (i.e., Diptera). These studies are burgeoning at an exponential rate, with an evident focus on mortality endpoint estimates, but they are also neglecting sublethal assessments. China and India in Asia, as well as Brazil in the Americas, were responsible for most studies. The majority of the papers used stored grain insects as experimental models, which limits the applicability and representativeness of the findings. As a result, the main modes of exposure tested were fumigation and contact, which leads to the prevalence of estimates of lethal concentration in these studies. Therefore, a broader range of insect species deserves testing, with suitable modes of exposure identifying and characterizing the main molecules responsible for the insecticidal activity, which is seldom performed. Attention to these needs will circumvent current biases and allow the recognition of the main patterns of association between the origin and structure of phytochemicals and their insecticidal effects.

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Management programs and efficient techniques are necessary to recover degraded ecosystems. The sewage sludge is rich in nitrogen (N) and with the potential to fertilize Sapindus saponaria L. (Sapinales: Sapindaceae), used in the recovery of degraded areas; this can affect the insect fauna. The study's objective was to evaluate, for 24 months, the abundance of chewing insects, dipterans, pollinators, and predators on S. saponaria plants fertilized with or without dehydrated sewage sludge in a degraded area. The experimental design was completely randomized (with the same characteristics) with two treatments (with or without dehydrated sewage sludge) and 24 replicates, each with one plant. The abundance of Anastrepha sp. (Tephritidae), Cerotoma sp. (Chrysomelidae), Curculionidae, Musca domestica L. (Muscidae), Mantis religiosa L. (Mantodea: Mantidae), Oxyopidae, Salticidae, Tettigoniidae (Orthoptera), and Teudis sp. (Anyphaenidae) was higher on fertilized plants. The abundances of Teudis sp. and Tmarus sp. (Thomisidae) and M. religiosa and Teudis sp. were positively correlated with chewing insects and Diptera, respectively. The population increase of insects and spiders on S. saponaria plants fertilized with dehydrated sewage sludge (bigger crowns) has shown to be suitable for recovering degraded areas with a higher number of niches and better food quality, improving the ecological indices of the area.

A degradação de ecossistemas torna necessário programas de manejo e técnicas eficientes para recuperá-los. O lodo de esgoto é rico em nitrogênio e com potencial para adubar Sapindus saponaria L. (Sapinales: Sapindaceae), utilizada na recuperação de áreas degradadas, mas isto pode afetar a fauna de insetos. O objetivo foi avaliar, durante 24 meses, abundância de insetos mastigadores, dípteros, polinizadores e predadores em plantas de S. saponaria fertilizadas ou não com lodo de esgoto desidratado, em área degradada. O delineamento experimental foi inteiramente casualisado com dois tratamentos (com ou sem lodo desidratado de esgoto) e 24 repetições, cada uma com uma planta. A abundância de Anastrepha sp. (Tephritidae), Cerotoma sp. (Chrysomelidae), Curculionidae, Musca domestica L. (Muscidae), Mantis religiosa L. (Mantodea: Mantidae), Oxyopidae, Salticidae, Tettigoniidae (Orthoptera) e Teudis sp. (Anyphaenidae) foi maior em plantas adubadas. A abundância de Teudis sp. e Tmarus sp. (Thomisidae) e M. religiosa e Teudis sp. foi, positivamente, correlacionada com as de insetos mastigadores e Diptera, respectivamente. O aumento populacional de insetos e aranhas em plantas de S. saponaria, adubada com lodo desidratado de esgoto (maiores copas), mostra ser adequado para a recuperação de áreas degradadas, tendo essas plantas um maior número de nichos e com alimento de melhor qualidade, melhorando os índices ecológicos na área.

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