RESUMO
Fish from both aquaculture and wild capture are exposed to veterinary and medicinal antibiotics (ABs). This study explored the occurrence and probable source of 46 antibiotic residues in muscle of farmed salmon and wild trout from Chile. Results showed that at least one AB was detected in all studied samples. Diverse patterns were observed between farmed and wild specimens, with higher ABs concentrations in wild fish. Considering antimicrobial resistance, detected ABs corresponded to the categories B (Restrict), C (Caution) and D (Prudence) established by Antimicrobial Advice Ad Hoc Expert Group (European Medicines Agency). Multivariate statistic was used to verify differences between farmed and wild populations, looking for the probable source of ABs as well. Principal components analysis (PCA) revealed that ciprofloxacin, moxifloxacin, enrofloxacin, amoxicillin, penicillin G, oxolinic acid, sulfamethoxazole, trimethoprim and clarithromycin were associated with wild samples, collected during the cold season. Conversely, norfloxacin, sulfaquinoxaline, sulfadimethoxine, nitrofurantoin, nalidixic acid, penicillin V, doxycycline, flumequine, oxacillin, pipemidic acid and sulfamethizole were associated with wild samples collected during the warm season. All farmed salmon samples were associated with ofloxacin, tetracycline, cephalexin, erythromycin, azithromycin, roxithromycin, sulfabenzamide, sulfamethazine, sulfapyridine, sulfisomidin, and sulfaguanidine. In addition, linear discriminant analysis showed that the AB profile in wild fish differ from farmed ones. Most samples showed ABs levels below the EU regulatory limit for edible fish, except for sulfaquinoxaline in one sample. Additionally, nitrofurantoin (banned in EU) was detected in one aquaculture sample. The differences observed between farmed and wild fish raise questions on the probable source of ABs, either aquaculture or urban anthropic activities. Further research is necessary for linking the ABs profile in wild fish with the anthropic source. However, to our knowledge, this is the first report showing differences in the ABs profile between wild and aquaculture salmonids, which could have both environmental and health consequences.
Assuntos
Antibacterianos , Salmonidae , Animais , Aquicultura , Chile , SalmãoRESUMO
To evaluate the physiological state of the wild fish inhabiting the Biobío River in South-Central Chile, susceptible to the chemical contamination from different sources, biochemical and physiological biomarkers were applied to wild fish Percilia irwini and Trichomycterus areolatus in situ. Fish caught in the Biobío river in low, medium, and high anthropic impacts areas, with different pollution degrees along the river. Ethoxyresorufin O-O deethylase (EROD) activity was evaluated in fish liver. Length, weight, Gonad weight and Liver weight, Physiological Index, and gill and liver histopathology were conducted. Physicochemical parameters (pH, Temperature, Conductivity, and TDS) were measured at each sampling site. The results indicated a deteriorating condition in the biological parameters of both species in a high anthropic zone. Fishes show an increase in physiological indices and EROD liver activity, agreeing with previous studies supporting evidence of reproductive change development as we move downstream the river. Also, an increase in histopathological lesions towards the lower third stretch of the Biobío River. The Integrated Index of Physiological Biomarkers (IPBR) indicated that sites located in the high impact area (P. irwini: BC: 4.09; RC: 3.38; PC: 3.50; SJ: 2.34 and T. areolatus BC: 6.06, PC: 5.37; SJ: 5.42) have the most detrimental environmental quality, compared to reference area. The integrated biomarker analysis demonstrates that the alterations observed are related to the high anthropic activity levels downstream from the sites with the least intervention, demonstrating that the IPBR used is a complementary tool for studies of the Environmental Effects Monitoring approach.
Assuntos
Monitoramento Ambiental , Poluentes Químicos da Água , Animais , Biomarcadores , Chile , Peixes , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Bioactive substances in the Biobio River Basin in Chile were examined by deploying Semi Permeable Membrane Devices (SPMDs) upstream and downstream of 4 pulp mill effluent discharges. Androgenic and estrogenic activity of SPMD extracts were then evaluated using in vitro fish sex steroid receptor binding assays. The results indicated the occurrence of estrogenic type compounds associated with one of the mill discharges. A significant correlation among the presence of these compounds, an increase in gonadosomatic index GSI and induction of hepatic EROD activity of two native fish species was observed. However, no significant presence of mature oocytes in female gonads was detected. Although EROD induction was observed in sites impacted by mill effluents, an increase of its activity occurred towards the downstream areas, suggesting other non-mill sources. More research is needed to understand the environmental changes in context of the new technological improvements in treatment systems to MBBR (Moving Bed Biofilm Reactor) recently implemented by the pulp mill industries.
Assuntos
Disruptores Endócrinos/toxicidade , Peixes/metabolismo , Resíduos Industriais/efeitos adversos , Receptores Androgênicos/metabolismo , Receptores de Estrogênio/metabolismo , Rios/química , Poluentes Químicos da Água/toxicidade , Animais , Reatores Biológicos , Chile , Citocromo P-450 CYP1A1/metabolismo , Disruptores Endócrinos/análise , Monitoramento Ambiental/métodos , Feminino , Peixes/crescimento & desenvolvimento , Resíduos Industriais/análise , Indústrias , Ligantes , Fígado/efeitos dos fármacos , Fígado/enzimologia , Reprodução/efeitos dos fármacos , Estações do Ano , Poluentes Químicos da Água/análise , Purificação da Água/métodosRESUMO
Abstract The fish stocking in captivity for later release into natural water bodies has historically been applied in Brazil, as a measure to mitigate impacts caused by dams. However, the released fish are adapted to the hatchery environment, which is totally different from the natural, which results in low post-release survival rates and ineffectiveness of stocking as a management measure. Worldwide, several studies have demonstrated the existence of large phenotypic differences between farmed and wild fish, but in Brazil, there is a great need for this kind of study. Based on this, our objective here was to verify if hatchery fish differ morphologically from its wild counterparties, at the same size class. Hence, a population of young Prochilodus lineatus raised in a fish farming station was compared with another from marginal lagoons of the Grande river. The results indicated that the fish farming resulted in morphologically different individuals of P. lineatus and a less diverse population, compared with wild fish. Measures such as environmental enrichment of hatchery tanks should be studied as a way to increase environmental heterogeneity and increase the morphological variability of the fingerlings produced.
Resumo A estocagem de peixes em cativeiro para posterior soltura em corpos d’água naturais tem sido historicamente aplicada no Brasil, como medida mitigadora de impactos causados por barramentos. Entretanto, os peixes soltos são adaptados ao ambiente de cultivo que é totalmente diferente do natural, o que resulta em pequenas taxas de sobrevivência pós-soltura e ineficácia das estocagens como medida de manejo. Em escala mundial, diversos estudos já evidenciaram a existência de grandes diferenças fenotípicas entre peixes cultivados e selvagens, mas no Brasil há grande carência de estudos deste tipo. O objetivo do presente estudo foi verificar se peixes cultivados divergem morfologicamente de indivíduos da mesma espécie provenientes de ambiente natural. Para isto, uma população de jovens de Prochilodus lineatus oriunda de uma estação de piscicultura foi comparada com outra da mesma espécie e classe de tamanho oriunda de lagoas marginais do Rio Grande. Os resultados encontrados indicaram que o cultivo em piscicultura resulta em indivíduos de P. lineatus morfologicamente diferentes e em uma população menos variada se comparada com os selvagens. Como forma de aumentar a heterogeneidade ambiental e aumentar a variabilidade morfológica dos alevinos produzidos, devem ser estudadas medidas como o enriquecimento ambiental de tanques de piscicultura.
Assuntos
Animais , Caraciformes/anatomia & histologia , Aquicultura , Brasil , Caraciformes/fisiologia , Pesqueiros , RiosRESUMO
Abstract The fish stocking in captivity for later release into natural water bodies has historically been applied in Brazil, as a measure to mitigate impacts caused by dams. However, the released fish are adapted to the hatchery environment, which is totally different from the natural, which results in low post-release survival rates and ineffectiveness of stocking as a management measure. Worldwide, several studies have demonstrated the existence of large phenotypic differences between farmed and wild fish, but in Brazil, there is a great need for this kind of study. Based on this, our objective here was to verify if hatchery fish differ morphologically from its wild counterparties, at the same size class. Hence, a population of young Prochilodus lineatus raised in a fish farming station was compared with another from marginal lagoons of the Grande river. The results indicated that the fish farming resulted in morphologically different individuals of P. lineatus and a less diverse population, compared with wild fish. Measures such as environmental enrichment of hatchery tanks should be studied as a way to increase environmental heterogeneity and increase the morphological variability of the fingerlings produced.(AU)
Resumo A estocagem de peixes em cativeiro para posterior soltura em corpos dágua naturais tem sido historicamente aplicada no Brasil, como medida mitigadora de impactos causados por barramentos. Entretanto, os peixes soltos são adaptados ao ambiente de cultivo que é totalmente diferente do natural, o que resulta em pequenas taxas de sobrevivência pós-soltura e ineficácia das estocagens como medida de manejo. Em escala mundial, diversos estudos já evidenciaram a existência de grandes diferenças fenotípicas entre peixes cultivados e selvagens, mas no Brasil há grande carência de estudos deste tipo. O objetivo do presente estudo foi verificar se peixes cultivados divergem morfologicamente de indivíduos da mesma espécie provenientes de ambiente natural. Para isto, uma população de jovens de Prochilodus lineatus oriunda de uma estação de piscicultura foi comparada com outra da mesma espécie e classe de tamanho oriunda de lagoas marginais do Rio Grande. Os resultados encontrados indicaram que o cultivo em piscicultura resulta em indivíduos de P. lineatus morfologicamente diferentes e em uma população menos variada se comparada com os selvagens. Como forma de aumentar a heterogeneidade ambiental e aumentar a variabilidade morfológica dos alevinos produzidos, devem ser estudadas medidas como o enriquecimento ambiental de tanques de piscicultura.(AU)
Assuntos
Animais , Caraciformes/anatomia & histologia , Caraciformes/crescimento & desenvolvimento , Pesqueiros , Variação Biológica da PopulaçãoRESUMO
We present the results of a field study that evaluates whether exposure to anthropogenic pollution impacts immunological and health-state parameters of wild marine fish during the breeding and non-breeding periods. We assessed aspects of innate immunity (bactericidal capacity, bacterial agglutination, and leukocyte profile) and general health-related parameters (neutrophil to lymphocyte ratio, hematocrit, and condition factor) in the Patagonian rockfish (Sebastes oculatus) sampled from polluted (exposed) and reference (control) sites during winter (i.e., coolest temperatures and active reproductive period) and in summer (i.e., warmest temperatures and non-reproductive period). Results showed lower bactericidal competence, hematocrit, and condition factor in fish from exposed sites independently of season, whereas lymphocytes were higher and monocytes lower at the exposed site only during summer. Moreover, fish sampled during winter displayed lower bactericidal competence, hematocrit, and condition factor than those sampled in summer independently of site, whereas the opposite pattern was found for bacterial agglutination. These results could be explained by life-history theory, which predicts a re-allocation of resources between reproduction and other physiological functions (including immunity) during the most energetically demanding season. The present results show an alteration in immunological and health-state parameters of wild marine fish exposed to anthropogenic pollution independently of season, which could potentially result in higher susceptibility to disease and in turn population decline.
Assuntos
Bass/imunologia , Testes de Aglutinação , Animais , Anticorpos/sangue , Argentina , Bass/sangue , Tamanho Corporal , Poluição Ambiental/efeitos adversos , Escherichia coli/imunologia , Hematócrito , Imunidade Inata , Contagem de Leucócitos , Estações do Ano , Poluentes Químicos da Água/efeitos adversosRESUMO
Abstract The fish stocking in captivity for later release into natural water bodies has historically been applied in Brazil, as a measure to mitigate impacts caused by dams. However, the released fish are adapted to the hatchery environment, which is totally different from the natural, which results in low post-release survival rates and ineffectiveness of stocking as a management measure. Worldwide, several studies have demonstrated the existence of large phenotypic differences between farmed and wild fish, but in Brazil, there is a great need for this kind of study. Based on this, our objective here was to verify if hatchery fish differ morphologically from its wild counterparties, at the same size class. Hence, a population of young Prochilodus lineatus raised in a fish farming station was compared with another from marginal lagoons of the Grande river. The results indicated that the fish farming resulted in morphologically different individuals of P. lineatus and a less diverse population, compared with wild fish. Measures such as environmental enrichment of hatchery tanks should be studied as a way to increase environmental heterogeneity and increase the morphological variability of the fingerlings produced.
Resumo A estocagem de peixes em cativeiro para posterior soltura em corpos dágua naturais tem sido historicamente aplicada no Brasil, como medida mitigadora de impactos causados por barramentos. Entretanto, os peixes soltos são adaptados ao ambiente de cultivo que é totalmente diferente do natural, o que resulta em pequenas taxas de sobrevivência pós-soltura e ineficácia das estocagens como medida de manejo. Em escala mundial, diversos estudos já evidenciaram a existência de grandes diferenças fenotípicas entre peixes cultivados e selvagens, mas no Brasil há grande carência de estudos deste tipo. O objetivo do presente estudo foi verificar se peixes cultivados divergem morfologicamente de indivíduos da mesma espécie provenientes de ambiente natural. Para isto, uma população de jovens de Prochilodus lineatus oriunda de uma estação de piscicultura foi comparada com outra da mesma espécie e classe de tamanho oriunda de lagoas marginais do Rio Grande. Os resultados encontrados indicaram que o cultivo em piscicultura resulta em indivíduos de P. lineatus morfologicamente diferentes e em uma população menos variada se comparada com os selvagens. Como forma de aumentar a heterogeneidade ambiental e aumentar a variabilidade morfológica dos alevinos produzidos, devem ser estudadas medidas como o enriquecimento ambiental de tanques de piscicultura.
Assuntos
Infecções por Alphavirus/veterinária , Alphavirus/isolamento & purificação , Doenças dos Peixes/virologia , Lagos , Infecções por Alphavirus/diagnóstico , Infecções por Alphavirus/epidemiologia , Infecções por Alphavirus/virologia , Animais , Chile/epidemiologia , Estudos Transversais , Feminino , Doenças dos Peixes/diagnóstico , Doenças dos Peixes/epidemiologia , Masculino , SalmãoRESUMO
Nearly 2,500 fries of Brycon cephalus with a standard length from 0.8 to 5.0cm were collected in Catalão Lake, on the right bank of Negro River and close to Marchantaria Island in Solimões River, in the State of Amazonas. A sample of 136 fishes was taken for parasitological examination and the remaining were placed in tanks at a fish fanning station for 30 days. Of the 136 fishes 49 (36%) were infected by trematodes metacercariae, 32 (23,5%) by the nematode Spirocamallanus inopinatus, 26 (19%) by the monogenoidea Jainus amazonensis, 3 (2%) by the protozoan Trichodina sp., 1 (0,7%) by the acanthocephala Echinorhynchus sp. and 2 (1,5%) by stages of cestoda larval. After 30 days a sample of 60 fish from the fish kept in the tanks was examined: 42 (70%) were infected by J. amazonensis, 14 (23%) by S. inopinatus, 9 (15%) by trematode metacercariae and 2 (3%) by Echinorhynchus sp. The highest average intensity and abundance values were observed related to J. amazonensis (342 and 65), followed by S. inopinatus (1 and 0,3), Trematoda (5 and 2), Cestoda (70 and 1), Echinorhynchus sp. (1 and 0,007) and Trichodina sp. (13 and 0,3). At the fish farming station the average intensity and abundance values were also higher for J. amazonensis (222 and 115) in relation to the other groups. On the other hand, J. amazonensis presented the highest average intensity and the lowest prevalence values in the wild. There was a variation on the parasitological fauna and infestation indexes after 30 days of permanence at the fish farming station.
Aproximadamente 2500 alevinos de matrinchã com comprimento padrão de 0,8 a 5,0cm foram coletados no lago Catalão, margem direita do rio Negro e no rio Solimões, próximo a ilha da Marchantaria, Estado do Amazonas. Uma subamostra de 136 peixes foi retirada para exames parasitológicos e o restante foi colocado em tanques em uma estação de piscicultura, durante 30 dias. Após esse período uma segunda amostra foi retirada e examinada. Dos 136 peixes: 49 (36%) estavam parasitados com metacercárias de trematódeos; 32 (23,5%) com nematóides Spirocamallanus inopinatus; 26 (19%) com monogenóideos Jainus amazonensis; 3 (2%) com protozoários Trichodina sp.; 1 (0,7%) com acantocéfalo Echinorhynchus sp. e 2 (1,5%) com estágios larvais de cestóides. Dos peixes mantidos nos tanques, após 30 dias foi examinada uma subamostra de 60 peixes: 42 (70%) estavam parasitados com J. amazonensis; 14 (23%) com S. inopinatus; 9 (15%) com metacercárias de trematódeos e 2 (3%) com Echinorhynchus sp. A intensidade média e abundância média foram maiores em J. amazonensis (342 e 65), seguido por cestóides (70 e 1), Trichodina sp. (13 e 0,3), trematódeos (5 e 2), S. inopinatus (1 e 0,3) e Echinorhynchus sp. (1 e 0,007). Na estação de piscicultura a intensidade média e abundância média também foram maiores para o J. amazonensis (222 e 115) em relação aos demais grupos. A espécie J. amazonensis apresentou maior intensidade média e menor prevalência na natureza. Houve variação na composição da fauna parasitógica e nos índices de infestação, após 30 dias de permanência na estação de piscicultura.
RESUMO
Nearly 2,500 fries of Brycon cephalus with a standard length from 0.8 to 5.0cm were collected in Catalão Lake, on the right bank of Negro River and close to Marchantaria Island in Solimões River, in the State of Amazonas. A sample of 136 fishes was taken for parasitological examination and the remaining were placed in tanks at a fish fanning station for 30 days. Of the 136 fishes 49 (36%) were infected by trematodes metacercariae, 32 (23,5%) by the nematode Spirocamallanus inopinatus, 26 (19%) by the monogenoidea Jainus amazonensis, 3 (2%) by the protozoan Trichodina sp., 1 (0,7%) by the acanthocephala Echinorhynchus sp. and 2 (1,5%) by stages of cestoda larval. After 30 days a sample of 60 fish from the fish kept in the tanks was examined: 42 (70%) were infected by J. amazonensis, 14 (23%) by S. inopinatus, 9 (15%) by trematode metacercariae and 2 (3%) by Echinorhynchus sp. The highest average intensity and abundance values were observed related to J. amazonensis (342 and 65), followed by S. inopinatus (1 and 0,3), Trematoda (5 and 2), Cestoda (70 and 1), Echinorhynchus sp. (1 and 0,007) and Trichodina sp. (13 and 0,3). At the fish farming station the average intensity and abundance values were also higher for J. amazonensis (222 and 115) in relation to the other groups. On the other hand, J. amazonensis presented the highest average intensity and the lowest prevalence values in the wild. There was a variation on the parasitological fauna and infestation indexes after 30 days of permanence at the fish farming station.
Aproximadamente 2500 alevinos de matrinchã com comprimento padrão de 0,8 a 5,0cm foram coletados no lago Catalão, margem direita do rio Negro e no rio Solimões, próximo a ilha da Marchantaria, Estado do Amazonas. Uma subamostra de 136 peixes foi retirada para exames parasitológicos e o restante foi colocado em tanques em uma estação de piscicultura, durante 30 dias. Após esse período uma segunda amostra foi retirada e examinada. Dos 136 peixes: 49 (36%) estavam parasitados com metacercárias de trematódeos; 32 (23,5%) com nematóides Spirocamallanus inopinatus; 26 (19%) com monogenóideos Jainus amazonensis; 3 (2%) com protozoários Trichodina sp.; 1 (0,7%) com acantocéfalo Echinorhynchus sp. e 2 (1,5%) com estágios larvais de cestóides. Dos peixes mantidos nos tanques, após 30 dias foi examinada uma subamostra de 60 peixes: 42 (70%) estavam parasitados com J. amazonensis; 14 (23%) com S. inopinatus; 9 (15%) com metacercárias de trematódeos e 2 (3%) com Echinorhynchus sp. A intensidade média e abundância média foram maiores em J. amazonensis (342 e 65), seguido por cestóides (70 e 1), Trichodina sp. (13 e 0,3), trematódeos (5 e 2), S. inopinatus (1 e 0,3) e Echinorhynchus sp. (1 e 0,007). Na estação de piscicultura a intensidade média e abundância média também foram maiores para o J. amazonensis (222 e 115) em relação aos demais grupos. A espécie J. amazonensis apresentou maior intensidade média e menor prevalência na natureza. Houve variação na composição da fauna parasitógica e nos índices de infestação, após 30 dias de permanência na estação de piscicultura.