RESUMO
BACKGROUND: Sugarcane silage has been increasing as a feed in the tropics by dairy farmers. However, sugarcane normally had high yeast population that leads to intense alcoholic fermentation and excessive Dry-Matter (DM) loss during ensilage and after air exposure, as well. There are several patents that have recently shown the benefits of applying Lactobacillus buchneri in forage preservation. OBJECTIVE: This study aimed to investigate the changes in pH, DM, Water-Soluble Carbohydrates (WSC) and fermentation end product concentrations that occur in sugarcane silage with or without inoculation with L. buchneri after 45 days of ensiling. METHODS: Sugarcane plants were harvested with approximately 16 months of growth and chopped at 2 cm. Four strains of wild L. buchneri (56.1, 56.4, 56.9 and 56.26) and the commercial inoculant "Lalsil Cana" were evaluated. For all treatments, the theoretical application rate was 1.0 × 106 colony- forming units (cfu) per g of fresh weight. Data from the silo openings were analysed as a completely randomized design, with four replicates per treatment (inoculants). RESULTS: The treatment with L. buchneri affected the DM content, pH, Lactic Acid Bacteria (LAB) population, DM recovery, and concentrations of WSC, lactic acid, acetic acid and ethanol of sugarcane silage after 45 days of ensiling. Yeasts and molds populations and the concentrations of propionic and butyric acids were not affected by the treatments. CONCLUSION: Lactobacillus buchneri 56.1 and 56.4 are considered the most suitable strains for improving the fermentation of sugarcane silage and thus are potential inoculants for silage production. At present, we are preparing the patent application.
Assuntos
Ração Animal/análise , Indústria de Laticínios/métodos , Fermentação , Lactobacillus , Saccharum/microbiologia , Silagem/microbiologia , Ração Animal/microbiologia , Fungos , Lactobacillus/classificação , Patentes como Assunto , Especificidade da Espécie , LevedurasRESUMO
The goal was to evaluate the chemical composition and fermentation pattern of elephant grass (Pennisetum purpureum Schum. cv. Roxo) silage with different levels of soybean hulls or rice bran. Two trials were conducted, comprising of a completely randomized design, with four replicates each. Treatments consisted on the addition of 0%, 5%, 10%, and 15% of soybean hulls or rice bran to unwilted green elephant grass forage. Large PVC silos were used adopting a density of 600 kg of green mass m-3. The silos were opened 40 days after ensiling. The results revealed that the inclusion of 10% soybean hulls increased elephant grass forage dry matter (DM) content to 31%, but did not alter the water soluble carbohydrate (WSC) content or buffering capacity. The resultant silages exhibited good fermentation patterns in terms of pH (less than 3.97) and NH3-N (4.07% total N) levels. The inclusion of rice bran increased both DM and WSC content in the forage, improving the fermentation pattern of silages (P < 0.05). This too was verified by a pH lower than 3.92 and a maximum NH3-N of 4.23% of the total N. The inclusion of 10% rice bran to the elephant grass improved the nutritional value of the forage to be ensiled and, hence, of the produced silage.(AU)
Objetivou-se avaliar a composição bromatológica e o padrão de fermentação da silagem de capim-elefante (Pennisetum purpureum Schum. cv. Roxo) aditivada com níveis de casca de soja e de farelo de arroz. Foram realizados dois experimentos para avaliar o uso de aditivos na ensilagem de capim elefante. Utilizou-se delineamento experimental inteiramente casualizado, com quatro repetições. Os tratamentos consistiram na adição de 0, 5, 10 e 15% do aditivo na massa verde de forragem de capim-elefante picada e sem emurchecimento. Foi utilizado silos de PVC, adotando densidade de 600 kg de massa verde m-3, com abertura do silo 40 dias após a ensilagem. A inclusão de 10% de casca de soja aumentou o teor de matéria seca (MS) da forragem de capim-elefante para 31%, porém não alterou os teores de carboidratos solúveis (CHOS) e poder tampão. As silagens de capim-elefante obtidas apresentaram bom padrão fermentativo, com valores de pH inferior a 3,97 e de N-NH3, 4,07% do N total. A inclusão de farelo de arroz proporcionou melhoria no padrão fermentativo das silagens produzidas, verificado pelo pH inferior a 3,92 e N-NH3 máximo de 4,23% do N total. A inclusão de 10% de farelo de arroz na ensilagem do capim-elefante melhorou o valor nutritivo da forragem a ser ensilada e, consequentemente, da silagem produzida.(AU)
Assuntos
Pennisetum , Silagem , FermentaçãoRESUMO
The goal was to evaluate the chemical composition and fermentation pattern of elephant grass (Pennisetum purpureum Schum. cv. Roxo) silage with different levels of soybean hulls or rice bran. Two trials were conducted, comprising of a completely randomized design, with four replicates each. Treatments consisted on the addition of 0%, 5%, 10%, and 15% of soybean hulls or rice bran to unwilted green elephant grass forage. Large PVC silos were used adopting a density of 600 kg of green mass m-3. The silos were opened 40 days after ensiling. The results revealed that the inclusion of 10% soybean hulls increased elephant grass forage dry matter (DM) content to 31%, but did not alter the water soluble carbohydrate (WSC) content or buffering capacity. The resultant silages exhibited good fermentation patterns in terms of pH (less than 3.97) and NH3-N (4.07% total N) levels. The inclusion of rice bran increased both DM and WSC content in the forage, improving the fermentation pattern of silages (P < 0.05). This too was verified by a pH lower than 3.92 and a maximum NH3-N of 4.23% of the total N. The inclusion of 10% rice bran to the elephant grass improved the nutritional value of the forage to be ensiled and, hence, of the produced silage.
Objetivou-se avaliar a composição bromatológica e o padrão de fermentação da silagem de capim-elefante (Pennisetum purpureum Schum. cv. Roxo) aditivada com níveis de casca de soja e de farelo de arroz. Foram realizados dois experimentos para avaliar o uso de aditivos na ensilagem de capim elefante. Utilizou-se delineamento experimental inteiramente casualizado, com quatro repetições. Os tratamentos consistiram na adição de 0, 5, 10 e 15% do aditivo na massa verde de forragem de capim-elefante picada e sem emurchecimento. Foi utilizado silos de PVC, adotando densidade de 600 kg de massa verde m-3, com abertura do silo 40 dias após a ensilagem. A inclusão de 10% de casca de soja aumentou o teor de matéria seca (MS) da forragem de capim-elefante para 31%, porém não alterou os teores de carboidratos solúveis (CHOS) e poder tampão. As silagens de capim-elefante obtidas apresentaram bom padrão fermentativo, com valores de pH inferior a 3,97 e de N-NH3, 4,07% do N total. A inclusão de farelo de arroz proporcionou melhoria no padrão fermentativo das silagens produzidas, verificado pelo pH inferior a 3,92 e N-NH3 máximo de 4,23% do N total. A inclusão de 10% de farelo de arroz na ensilagem do capim-elefante melhorou o valor nutritivo da forragem a ser ensilada e, consequentemente, da silagem produzida.
Assuntos
Fermentação , Pennisetum , SilagemRESUMO
The water soluble carbohydrates (WSC) glucose, fructose, and sucrose are well-known to the great public, but fructans represent another type of WSC that deserves more attention given their prebiotic and immunomodulatory properties in the food context. Although the occurrence of inulin-type fructo-oligosaccharides (FOS) was proposed in the fruit of some banana accessions, little or no information is available neither on the exact identity of the fructan species, nor on the fructan content in different parts of banana plants and among a broader array of banana cultivars. Here, we investigated the WSC composition in leaves, pulp of ripe fruits and rhizomes from mature banana plants of 11 accessions (I to XI), including both cultivated varieties and wild Musa species. High performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD) showed the presence of 1-kestotriose [GF2], inulobiose [F2], inulotriose [F3], 6-kestotriose and 6G-kestotriose (neokestose) fructan species in the pulp of mature fruits of different accessions, but the absence of 1,1-nystose and 1,1,1 kestopentaose and higher degree of polymerization (DP) inulin-type fructans. This fructan fingerprint points at the presence of one or more invertases that are able to use fructose and sucrose as alternative acceptor substrates. Quantification of glucose, fructose, sucrose and 1-kestotriose and principal component analysis (PCA) identified related banana groups, based on their specific WSC profiles. These data provide new insights in the biochemical diversity of wild and cultivated bananas, and shed light on potential roles that fructans may fulfill across species, during plant development and adaptation to changing environments. Furthermore, the promiscuous behavior of banana fruit invertases (sucrose and fructose as acceptor substrates besides water) provides a new avenue to boost future work on structure-function relationships on these enzymes, potentially leading to the development of genuine banana fructosyltransferases that are able to increase fructan content in banana fruits.
RESUMO
Sugar cane silages are characterized by extensive yeast activity, alcohol production and great dry matter - DM - losses. Better knowledge of the fermentation process is fundamental to the development of efficient ensilage techniques for this forage. This study evaluates temporal changes in chemical composition, DM losses and epiphytic microflora in sugar cane silage. Mature sugar cane, variety RB835486 (12 months of vegetative growth), was hand harvested, processed in a stationary chopper and ensiled in 20-L plastic buckets provided with valves for gas release and a device for effluent collection. Laboratory silos were kept at ambient temperature and sampled after ½, 1, 2, 3, 7, 15, 45, 90, 120 and 180 days. Ethanol concentration reached 6.4% in DM after 15 days of ensilage, followed by 71% water soluble carbohydrates - WSCs - disappearance. Gas and total DM losses reached a plateau on day 45 (16% and 29% of DM, respectively). Yeast count was higher on the second day (5.05 log cfu g-1). Silage pH declined to below 4.0 on the third day. Effluent yield was negligible (20 kg t-1). DM content in the forage decreased (35% to 26%) from day 0 to day 45. The increase in ethanol concentration showed an opposite trend to WSCs and true in vitro dry matter digestibility reductions in the silage. Developing methods to control yeasts, most probably through the use of additives, will enable more efficient production of sugar cane silage by farmers.
Silagens de cana-de-açúcar caracterizam-se pela extensa atividade de leveduras, alto teor de álcool e grandes perdas de matéria seca - MS. Conhecer melhor o processo fermentativo é fundamental para o desenvolvimento de técnicas eficientes de ensilagem da cana. Este trabalho avalia a mudança temporal na composição química, nas perdas de MS e na microflora epífita nestas silagens. Cana-de-açúcar (RB835486) foi colhida manualmente (12 meses de crescimento), picada em picadora estacionária e ensilada em baldes de plástico de 20 L com válvulas para gases e aparato para colheita de efluentes. Os silos laboratoriais foram mantidos sob temperatura ambiente e amostrados após ½, 1, 2, 3, 7, 15, 45, 90, 120 e 180 dias. Etanol atingiu 6,4% na MS no 15º dia após ensilagem, seguido pelo desaparecimento de 71% dos carboidratos solúveis - CHOs. As perdas gasosas e a perda total de MS estabilizaram-se após 45 dias (16% e 29% da MS). A contagem de leveduras foi máxima no segundo dia (5,05 log ufc g-1). O pH atingiu nível abaixo de 4,0 no terceiro dia. A produção de efluentes foi insignificante (20,1 kg t-1). O teor de MS da forragem decresceu (35% para 26%) do dia 0 ao 45º dia. O padrão de variação na concentração de etanol foi inverso à concentração de CHOs e à redução da digestibilidade da silagem. O desenvolvimento de métodos de controle das leveduras, provavelmente com o uso de aditivos, melhorará a eficiência no uso de silagens de cana-de-açúcar pelos pecuaristas.
RESUMO
Sugar cane silages are characterized by extensive yeast activity, alcohol production and great dry matter - DM - losses. Better knowledge of the fermentation process is fundamental to the development of efficient ensilage techniques for this forage. This study evaluates temporal changes in chemical composition, DM losses and epiphytic microflora in sugar cane silage. Mature sugar cane, variety RB835486 (12 months of vegetative growth), was hand harvested, processed in a stationary chopper and ensiled in 20-L plastic buckets provided with valves for gas release and a device for effluent collection. Laboratory silos were kept at ambient temperature and sampled after ½, 1, 2, 3, 7, 15, 45, 90, 120 and 180 days. Ethanol concentration reached 6.4% in DM after 15 days of ensilage, followed by 71% water soluble carbohydrates - WSCs - disappearance. Gas and total DM losses reached a plateau on day 45 (16% and 29% of DM, respectively). Yeast count was higher on the second day (5.05 log cfu g-1). Silage pH declined to below 4.0 on the third day. Effluent yield was negligible (20 kg t-1). DM content in the forage decreased (35% to 26%) from day 0 to day 45. The increase in ethanol concentration showed an opposite trend to WSCs and true in vitro dry matter digestibility reductions in the silage. Developing methods to control yeasts, most probably through the use of additives, will enable more efficient production of sugar cane silage by farmers.
Silagens de cana-de-açúcar caracterizam-se pela extensa atividade de leveduras, alto teor de álcool e grandes perdas de matéria seca - MS. Conhecer melhor o processo fermentativo é fundamental para o desenvolvimento de técnicas eficientes de ensilagem da cana. Este trabalho avalia a mudança temporal na composição química, nas perdas de MS e na microflora epífita nestas silagens. Cana-de-açúcar (RB835486) foi colhida manualmente (12 meses de crescimento), picada em picadora estacionária e ensilada em baldes de plástico de 20 L com válvulas para gases e aparato para colheita de efluentes. Os silos laboratoriais foram mantidos sob temperatura ambiente e amostrados após ½, 1, 2, 3, 7, 15, 45, 90, 120 e 180 dias. Etanol atingiu 6,4% na MS no 15º dia após ensilagem, seguido pelo desaparecimento de 71% dos carboidratos solúveis - CHOs. As perdas gasosas e a perda total de MS estabilizaram-se após 45 dias (16% e 29% da MS). A contagem de leveduras foi máxima no segundo dia (5,05 log ufc g-1). O pH atingiu nível abaixo de 4,0 no terceiro dia. A produção de efluentes foi insignificante (20,1 kg t-1). O teor de MS da forragem decresceu (35% para 26%) do dia 0 ao 45º dia. O padrão de variação na concentração de etanol foi inverso à concentração de CHOs e à redução da digestibilidade da silagem. O desenvolvimento de métodos de controle das leveduras, provavelmente com o uso de aditivos, melhorará a eficiência no uso de silagens de cana-de-açúcar pelos pecuaristas.