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Some Escherichia coli serotypes are important human pathogens causing diarrhea or in some cases, life threatening diseases. E. coli is also a typical indicator microorganism, routinely used for assessing the microbiological quality of water especially to indicate fecal contamination. The soil is a sink and route of transmission to water and food resources and it is thus important to understand the survival of enterotoxigenic E. coli strains in soil. This study monitored the survival of six E. coli strains in sandy and loam soil. Furthermore, since biochar is a commonly used soil conditioner, the study investigated the impact of biochar amendment (15%) on the survival of the E. coli strains in (biochar-amended) sandy and loam soils. Addition of biochar affected the physicochemical properties of both soils, altering potassium levels, calcium, magnesium, sodium as well as levels of other metal ions. It increased the organic matter of loam soil from 44 g/dm3 to 52 g/dm3, and increased the pH of both sandy and loam soils. Survival and persistence of the E. coli strains generally varied according to soil type, with strains generally surviving better (P ≤ 0.05) in loam soil compared to in sandy soil. In loam soil and biochar amended loam soils, E. coli strains remained culturable until the 150th day with counts ranging between 3.00 and 5.94 ± 0.04 log CFU/g. The effects of biochar on the physicochemical properties of soil and the response of the E. coli strains to biochar amendment was variable depending on soil type.

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INTRODUCTION: Solar disinfection (SODIS) is an effective method for microbiologic inactivation of contaminated water using ultraviolet rays at low elevations. The aim of this study was to determine the effectiveness of SODIS at higher elevations. METHODS: The ability of SODIS to inactivate Escherichia coli bacteria was evaluated at an altitude of ≥1600 m using Nalgene bottles, disposable plastic water bottles, and Ziploc plastic bags. Bacterial viability was determined through measurement of colony forming units (CFUs). Decreases in CFUs were determined at each time point relative to those at the baseline, and a multivariable regression analysis was used to assess significant changes in CFUs. RESULTS: Bacterial CFUs in exposed containers decreased by >5 log after 6 h of exposure to sunlight. In contrast, the CFUs remained nearly unchanged in unexposed containers, showing a mean decrease of 0.3 log. By 2 h, bacterial inactivation at high altitudes was 1.7-fold greater than that at lower altitudes (P<0.05). By 6 h, nearly all bacteria were inactivated at high or low altitudes. At 6 h, no statistical difference was observed in the efficiency of inactivation between elevations. Compared with Nalgene bottles, plastic bottles had a 1.4-fold greater decrease in CFUs (P<0.05). No statistical difference in bacterial inactivation was found between plastic bottles and plastic bags. CONCLUSIONS: At high altitudes, SODIS is an effective method for inactivating E coli. Further research investigating other microorganisms is warranted to determine whether SODIS is suitable for disinfecting contaminated water at high altitudes.

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BACKGROUND: Fluoride is an inorganic element, which can be found in high concentrations in groundwater. Its consumption and exposure have consequences on human health. The objective of this study was to evaluate fluoride exposure and develop a health risk assessment in children from an urban area with hydrofluorosis in Mexico. METHODS: Water fluoride levels in active wells were provided by the Water State Agency and divided into three zones: agriculture zone (Zone A), metallurgical zone (Zone B), and industrial zone (Zone C). Urinary fluoride levels were determined by potentiometric method using an ion-selective electrode. Health risk assessment was performed through Monte Carlo model analysis and hazard quotient was calculated. RESULTS: According to fluoride well concentration, all zones have high concentration especially Zone B (2.55 ± 0.98 mg/L). Urinary fluoride concentrations were highest in children in Zone B (1.42 ± 0.8 mg/L). The estimated median daily intake dose of fluoride was 0.084 mg/Kg-day for the children living in zone B. The highest mean HQ value was to Zone B (1.400 ± 0.980), followed by Zone C (0.626 ± 0.443). CONCLUSION: The levels of fluoride exposure registered are a potential risk to generate adverse health effects in children in the San Luis Potosi metropolitan area.

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RESUMO O Plano de Segurança da Água (PSA) contempla, entre outras etapas, a identificação e o acompanhamento de eventos perigosos e pode garantir qualidade hídrica aos consumidores. A ruralidade abrange cerca de 15% da população brasileira e o acompanhamento técnico é insuficiente para atender às demandas locais. Nesse contexto, o principal objetivo foi elaborar um modelo conceitual de PSA para monitoramento de riscos à contaminação da água na área rural, contemplando eventos desde a captação até a distribuição de água em comunidades rurais. A metodologia consistiu em caracterização de eventos perigosos, concepção do modelo computacional, consulta a especialistas e calibração e aplicação do modelo em propriedades rurais. Entre os resultados, tem-se um software elaborado em Python contendo 109 eventos direcionados ao abastecimento rural. O programa analisou a precariedade no entorno da captação de água e as condições de limpeza das propriedades estudadas. Os resultados indicaram que os riscos mais elevados, em sua maioria, são provenientes do uso de fossa negra, descarte irregular de resíduos sólidos, lançamento de esgoto doméstico a céu aberto e criação de animais nas proximidades da captação de água. Não há softwares disponíveis para mensuração de riscos ao abastecimento de água em áreas rurais e o modelo proposto pode ser utilizado para avaliação da salubridade rural no contexto nacional. Dessa forma, o software subsidia o monitoramento dos recursos hídricos e amplia a capacidade de gerenciamento no setor.

ABSTRACT The Water Safety Plan (WSP) includes, among other steps, the identification and monitoring of dangerous events can guarantee water quality to consumers. Rurality covers about 15% of the Brazilian population and technical support is insufficient to assist local demands. In this context, the main objective was to develop a conceptual WSP model for monitoring water contamination risks in the rural area, contemplating events from water catchment to water distribution in rural communities. The methodology consisted of the characterization of dangerous events, conception of the computational model, specialists consultation, and model calibration and application in rural properties. Among the results, there is a software elaborated in Python containing 109 events aimed at rural supply. The program analyzed the precariousness around the water catchment and the clean conditions of the studied properties. These results indicated the highest risks, for the most part, come from the use of cesspool, irregular disposal of solid waste, domestic sewage discharge, and animal beeding nearby water catchment. There is no software available to measure water supply risks in rural areas and the proposed model can be used for environmental health evaluatiation in the national context. In this way, the software can support water resource monitoring and expands the manageability in the sector.

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Access to safe drinking water is limited in many isolated areas, such as the Amazon where Indigenous peoples frequently reside. Identifying safe forms of drinking water accepted by the communities could have positive health benefits for Indigenous peoples. Many Amazon Indigenous peoples traditionally prepare and consume a fermented beverage called masato, which is frequently the only form of water consumption. Despite its widespread consumption and evidence of the health benefits of fermentation, masato remains poorly investigated. We partnered with a Shawi Indigenous community in the Peruvian Amazon to conduct participatory photography to research masato preparation, and to characterize key cultural features and to assess the presence of total and fecal coliform bacteria by using a membrane filter technique. Pictures show that masato preparation is a key part of cultural practices and that there are clear gender roles in the preparation process. We found that 100% of communal water sources (26/26) were contaminated with coliform bacteria; by contrast, fewer, 18% of masato samples (2/11), were positive for coliform. This exploratory study suggests that fermented beverages like masato merit further investigation as they represent an Indigenous method to improve water quality in Amazonian communities where water safety cannot be assured.

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The water-safety plan (WSP) approach integrates risk assessment and management practices into a drinking-water supply system (DWSS), ensuring water quality from the catchment to the consumer. This research was oriented toward the development and implementation of a WSP in the DWSS for the city of Santiago de Cali, Colombia. We adapted the methodological stages of the WSP established by the World Health Organization: (i.) assemble a WSP team, (ii.) describe the existing DWSS, (iii.) identify hazards and hazardous events, (iv.) evaluate risks, and (v.) plan risk management. In this methodological structure, we propose the use of different risk assessment tools at each step of DWSS, which can be implemented in water systems of different sizes. The results of our study allowed the identification of hazardous events that may compromise the effectiveness of the WSP: Of the 103 events found, 16 were related to the catchment, 40 to treatment processes, 27 to the distribution system, and 9 to consumers, while the remaining 11 were associated with organizational, planning, contingency, and emergency factors. We found that the most critical components of the DWSS are the distribution system and the consumers, with 29.6% and 33.3% of the hazardous events classified as at a very high risk level, respectively. Clearly, improvement and support programs for the WSP were needed to optimize existing control measures and corrective actions and to evaluate new ones to reduce risk levels. The treatment processes and distribution system steps were shown as the main barriers of the DWSS mainly they are under governance of water company, unlike the catchment and consumers which presented higher levels of risk because in these steps the water company has less control and influence. Our research confirms that the WSP is an important tool for decision-making by water-service companies-improving their administrative, financial, organizational, and operational management. It also shows that it is essential that their senior management and other stakeholders be part of the WSP team at each stage of implementation of the DWSS.

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Knowledge of the quantity and quality of the material lost by soil erosion due to soil management is a basic need to identify land management zones in catchments. The aim of this study was to investigate the influence of soil management on the quantity and quality of soil material lost by erosion in the Posses sub-watershed, Municipality of Extrema, State of Minas Gerais, Brazil. Water and sediments lost by natural rainfall erosion were sampled from erosion plots located on a Red-Yellow Argisol (PVA) under the following systems: bare soil, subsistence farming (maize/beans/pumpkin/jack-beans/fallow), degraded pasture, well-managed pasture, and reforestation set up in 2013; and in a Litholic Neosol (RL): reforestation set up in 2008, bare soil, and native forest. Ca, Mg, K, P, N and soil organic matter (SOM) contents were determined in sediment and soil samples (at 0-5 cm depth) for the determination of the runoff enrichment ratios. Management influences soil losses more so than water losses. Minor losses were found in reforestation set up in 2013 (soil); in well-managed pasture (water); and in reforestations (nutrients and SOM). These losses tend to stability with time. The general sequence of nutrient losses was N > Ca > Mg > K > P in PVA; and N > Ca > K > Mg > P in RL. Loss rates of SOM and N followed the order: bare soil > subsistence farming > degraded pasture > well-managed pasture >reforestation, in PVA; and bare soil > native forest > reforestation, in Argisol. Reforestation and wellmanagementpasture are effective conservation strategies in order to lower the erosion process in thePosses sub-watershed. Soil losses, as well as nutrients and organic matter losses were more influencedby soil management than water losses. The safeguarding native forest under Argisol is essential to theconservation of this pedoenvironment, especially in steep slopes.(AU)

O conhecimento da quantidade e qualidade de material perdido por erosão hídrica em função do manejo do solo constitui uma necessidade básica para a definição de zonas de manejo em áreas de recarga de água. O objetivo desse trabalho foi verificar a influência do manejo do solo sobre a quantidade e qualidade de material perdido por erosão hídrica na sub-bacia hidrográfica das Posses, Extrema-MG. Para isso, foram coletados água e sedimentos erodidos através de parcelas de erosão, instaladas, sob chuva natural, em Argissolo Vermelho-Amarelo (PVA) nos manejos: solo descoberto, rotação cultural (milho/feijão-de-porco/pousio), pastagens degradada e bem manejada, e reflorestamento implantado em 2013; e em Neossolo Litólico (RL) nos manejos: reflorestamento implantado em 2008, solo descoberto e mata nativa. Nas amostras de sedimentos e de solos (coletadas na profundidade de 0-5 cm) foram determinados os teores de Ca, Mg, K, P, N (NT) e de matéria orgânica do solo (MOS) e com isso, calculada a taxa de enriquecimento de enxurrada. O manejo exerce maior influência nas perdas de solo do que nas perdas de água. Menores perdas foram encontradas no reflorestamento implantado em 2013 (solo), pasto bem manejado (água) e reflorestamentos (nutrientes e matéria orgânica). Há uma tendência de estabilização dessas perdas com o tempo de implantação dos sistemas. A tendência geral das perdas de nutrientesno PVA foi NT > Ca > Mg > K > P; e no Argisol foi de NT > Ca > K > Mg > P. As taxas de perdasde MOS e NT seguem a ordem: solo descoberto > rotação cultural > pasto degradado > pasto bemmanejado > reflorestamento, no PVA; e solo descoberto > mata nativa > reflorestamento, no Argisol. Oreflorestamento e o manejo das pastagens são estratégias conservacionistas que minimizam as perdaspor erosão hídrica na sub-bacia das Posses, Extrema-MG. O manejo exerce maior influencia nas perdas de solo e de materiais a ele relacionados do que nas perdas de água. A manutenção da mata nativa sob...(AU)

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Knowledge of the quantity and quality of the material lost by soil erosion due to soil management is a basic need to identify land management zones in catchments. The aim of this study was to investigate the influence of soil management on the quantity and quality of soil material lost by erosion in the Posses sub-watershed, Municipality of Extrema, State of Minas Gerais, Brazil. Water and sediments lost by natural rainfall erosion were sampled from erosion plots located on a Red-Yellow Argisol (PVA) under the following systems: bare soil, subsistence farming (maize/beans/pumpkin/jack-beans/fallow), degraded pasture, well-managed pasture, and reforestation set up in 2013; and in a Litholic Neosol (RL): reforestation set up in 2008, bare soil, and native forest. Ca, Mg, K, P, N and soil organic matter (SOM) contents were determined in sediment and soil samples (at 0-5 cm depth) for the determination of the runoff enrichment ratios. Management influences soil losses more so than water losses. Minor losses were found in reforestation set up in 2013 (soil); in well-managed pasture (water); and in reforestations (nutrients and SOM). These losses tend to stability with time. The general sequence of nutrient losses was N > Ca > Mg > K > P in PVA; and N > Ca > K > Mg > P in RL. Loss rates of SOM and N followed the order: bare soil > subsistence farming > d

O conhecimento da quantidade e qualidade de material perdido por erosão hídrica em função do manejo do solo constitui uma necessidade básica para a definição de zonas de manejo em áreas de recarga de água. O objetivo desse trabalho foi verificar a influência do manejo do solo sobre a quantidade e qualidade de material perdido por erosão hídrica na sub-bacia hidrográfica das Posses, Extrema-MG. Para isso, foram coletados água e sedimentos erodidos através de parcelas de erosão, instaladas, sob chuva natural, em Argissolo Vermelho-Amarelo (PVA) nos manejos: solo descoberto, rotação cultural (milho/feijão-de-porco/pousio), pastagens degradada e bem manejada, e reflorestamento implantado em 2013; e em Neossolo Litólico (RL) nos manejos: reflorestamento implantado em 2008, solo descoberto e mata nativa. Nas amostras de sedimentos e de solos (coletadas na profundidade de 0-5 cm) foram determinados os teores de Ca, Mg, K, P, N (NT) e de matéria orgânica do solo (MOS) e com isso, calculada a taxa de enriquecimento de enxurrada. O manejo exerce maior influência nas perdas de solo do que nas perdas de água. Menores perdas foram encontradas no reflorestamento implantado em 2013 (solo), pasto bem manejado (água) e reflorestamentos (nutrientes e matéria orgânica). Há uma tendência de estabilização dessas perdas com o tempo de implantação dos sistemas. A tendência geral das perdas de nutrien

RESUMO

Knowledge of the quantity and quality of the material lost by soil erosion due to soil management is a basic need to identify land management zones in catchments. The aim of this study was to investigate the influence of soil management on the quantity and quality of soil material lost by erosion in the Posses sub-watershed, Municipality of Extrema, State of Minas Gerais, Brazil. Water and sediments lost by natural rainfall erosion were sampled from erosion plots located on a Red-Yellow Argisol (PVA) under the following systems: bare soil, subsistence farming (maize/beans/pumpkin/jack-beans/fallow), degraded pasture, well-managed pasture, and reforestation set up in 2013; and in a Litholic Neosol (RL): reforestation set up in 2008, bare soil, and native forest. Ca, Mg, K, P, N and soil organic matter (SOM) contents were determined in sediment and soil samples (at 0-5 cm depth) for the determination of the runoff enrichment ratios. Management influences soil losses more so than water losses. Minor losses were found in reforestation set up in 2013 (soil); in well-managed pasture (water); and in reforestations (nutrients and SOM). These losses tend to stability with time. The general sequence of nutrient losses was N > Ca > Mg > K > P in PVA; and N > Ca > K > Mg > P in RL. Loss rates of SOM and N followed the order: bare soil > subsistence farming > degraded pasture > well-managed pasture >reforestation, in PVA; and bare soil > native forest > reforestation, in Argisol. Reforestation and wellmanagementpasture are effective conservation strategies in order to lower the erosion process in thePosses sub-watershed. Soil losses, as well as nutrients and organic matter losses were more influencedby soil management than water losses. The safeguarding native forest under Argisol is essential to theconservation of this pedoenvironment, especially in steep slopes.

O conhecimento da quantidade e qualidade de material perdido por erosão hídrica em função do manejo do solo constitui uma necessidade básica para a definição de zonas de manejo em áreas de recarga de água. O objetivo desse trabalho foi verificar a influência do manejo do solo sobre a quantidade e qualidade de material perdido por erosão hídrica na sub-bacia hidrográfica das Posses, Extrema-MG. Para isso, foram coletados água e sedimentos erodidos através de parcelas de erosão, instaladas, sob chuva natural, em Argissolo Vermelho-Amarelo (PVA) nos manejos: solo descoberto, rotação cultural (milho/feijão-de-porco/pousio), pastagens degradada e bem manejada, e reflorestamento implantado em 2013; e em Neossolo Litólico (RL) nos manejos: reflorestamento implantado em 2008, solo descoberto e mata nativa. Nas amostras de sedimentos e de solos (coletadas na profundidade de 0-5 cm) foram determinados os teores de Ca, Mg, K, P, N (NT) e de matéria orgânica do solo (MOS) e com isso, calculada a taxa de enriquecimento de enxurrada. O manejo exerce maior influência nas perdas de solo do que nas perdas de água. Menores perdas foram encontradas no reflorestamento implantado em 2013 (solo), pasto bem manejado (água) e reflorestamentos (nutrientes e matéria orgânica). Há uma tendência de estabilização dessas perdas com o tempo de implantação dos sistemas. A tendência geral das perdas de nutrientesno PVA foi NT > Ca > Mg > K > P; e no Argisol foi de NT > Ca > K > Mg > P. As taxas de perdasde MOS e NT seguem a ordem: solo descoberto > rotação cultural > pasto degradado > pasto bemmanejado > reflorestamento, no PVA; e solo descoberto > mata nativa > reflorestamento, no Argisol. Oreflorestamento e o manejo das pastagens são estratégias conservacionistas que minimizam as perdaspor erosão hídrica na sub-bacia das Posses, Extrema-MG. O manejo exerce maior influencia nas perdas de solo e de materiais a ele relacionados do que nas perdas de água. A manutenção da mata nativa sob...

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Monitoring of progress towards the Millennium Development Goal (MDG) drinking water target relies on classification of water sources as "improved" or "unimproved" as an indicator for water safety. We adjust the current Joint Monitoring Programme (JMP) estimate by accounting for microbial water quality and sanitary risk using the only-nationally representative water quality data currently available, that from the WHO and UNICEF "Rapid Assessment of Drinking Water Quality". A principal components analysis (PCA) of national environmental and development indicators was used to create models that predicted, for most countries, the proportions of piped and of other-improved water supplies that are faecally contaminated; and of these sources, the proportions that lack basic sanitary protection against contamination. We estimate that 1.8 billion people (28% of the global population) used unsafe water in 2010. The 2010 JMP estimate is that 783 million people (11%) use unimproved sources. Our estimates revise the 1990 baseline from 23% to 37%, and the target from 12% to 18%, resulting in a shortfall of 10% of the global population towards the MDG target in 2010. In contrast, using the indicator "use of an improved source" suggests that the MDG target for drinking-water has already been achieved. We estimate that an additional 1.2 billion (18%) use water from sources or systems with significant sanitary risks. While our estimate is imprecise, the magnitude of the estimate and the health and development implications suggest that greater attention is needed to better understand and manage drinking water safety.

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Water from the cup filler of dental chair units (CFDC) was observed to contain sphingomonads, environmental mycobacteria and methylobacteria, among other minor bacteria. Some of the bacteria detected are recognized opportunistic pathogens. Some of these, tended to persist over time.

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Water from the cup filler of dental chair units (CFDC) was observed to contain sphingomonads, environmental mycobacteria and methylobacteria, among other minor bacteria. Some of the bacteria detected are recognized opportunistic pathogens. Some of these, tended to persist over time.

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Water from the cup filler of dental chair units (CFDC) was observed to contain sphingomonads, environmental mycobacteria and methylobacteria, among other minor bacteria. Some of the bacteria detected are recognized opportunistic pathogens. Some of these, tended to persist over time.