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INTRODUCTION: In some studies, the peritoneal solute transfer rate (PSTR) through the peritoneal membrane has been related to an increased risk of mortality. It has been observed in the literature that those patients with rapid diffusion of solutes through the peritoneal membrane (high/fast transfer) and probably those with high average transfer characterized by the Peritoneal Equilibrium Test (PET) are associated with higher mortality compared to those patients who have a slow transfer rate. However, some authors have not documented this fact. In the present study, we want to evaluate the (etiological) relationship between the characteristics of peritoneal membrane transfer and mortality and survival of the technique in an incident population on peritoneal dialysis in RTS Colombia during the years 2007-2017 using a competing risk model. MATERIALS AND METHODS: A retrospective cohort study was carried out at RTS Colombia in the period between 2007 and 2017. In total, there were 8170 incident patients older than 18 years, who had a Peritoneal Equilibration Test (PET) between 28 and 180 days from the start of therapy. Demographic, clinical, and laboratory variables were evaluated. The (etiological) relationship between the type of peritoneal solute transfer rate at the start of therapy and overall mortality and technique survival were analyzed using a competing risk model (cause-specific proportional hazard model described by Royston-Lambert). RESULTS: Patients were classified into four categories based on the PET result: Slow/Low transfer (16.0%), low average (35.4%), high average (32.9%), and High/Fast transfer (15.7%). During follow-up, with a median of 730 days, 3025 (37.02%) patients died, 1079 (13.2%) were transferred to hemodialysis and 661 (8.1%) were transplanted. In the analysis of competing risks, adjusted for age, sex, presence of DM, HTA, body mass index, residual function, albumin, hemoglobin, phosphorus, and modality of PD at the start of therapy, we found cause-specific HR (HRce) for high/fast transfer was 1.13 (95% CI 0.98-1.30) p = 0.078, high average 1.08 (95% CI 0.96-1.22) p = 0.195, low average 1.09 (95% CI 0.96-1.22) p = 0.156 compared to the low/slow transfer rate. For technique survival, cause-specific HR for high/rapid transfer of 1.22 (95% CI 0.98-1.52) p = 0.66, high average HR was 1.10 (95% CI 0.91-1.33) p = 0.296, low average HR of 1.03 (95% CI 0.85-1.24) p = 0.733 compared with the low/slow transfer rate, adjusted for age, sex, DM, HTA, BMI, residual renal function, albumin, phosphorus, hemoglobin, and PD modality at start of therapy. Non-significant differences. CONCLUSIONS: When evaluating the etiological relationship between the type of peritoneal solute transfer rate and overall mortality and survival of the technique using a competing risk model, we found no etiological relationship between the characteristics of peritoneal membrane transfer according to the classification given by Twardowski assessed at the start of peritoneal dialysis therapy and overall mortality or technique survival in adjusted models. The analysis will then be made from the prognostic model with the purpose of predicting the risk of mortality and survival of the technique using the risk subdistribution model (Fine & Gray).

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This work presents an experimental evaluation of a Venturi and Venturi-Vortex microbubble aeration system, taking as input variables the water-air flow ratio, water renewal time and area-volume ratio of the water tank. The aeration process response variables are defined in terms of oxygen transfer and aeration efficiency through the standard volumetric mass transfer coefficient (KLa20), standard oxygen transfer rate (SOTR), and standard aeration efficiency (SAE). Two methods of air injection were analyzed: 1. Air injection in the throat chamber of the Venturi generator; 2. air supplying in the suction side of the hydraulic pump of the aeration system. Experimental results indicate that the water renewal time variable (RT) is a statistically significant factor with respect to the KLa20, which can be maximized by decreasing RT. The effects of the variable flow ratio (FR) are greater than the effects of renewal time and area-volume ratio (AVR) concerning SOTR and SAE, indicating a maximum response with a minimum flow ratio, using the Venturi-Vortex microbubble generator. When the flow ratio decreases, the air flow increases, generating and transferring a greater amount of microbubbles (MB) into the water. It was found that increasing the air flow produced an increase in the standard oxygen transfer rate SOTR and standard aeration efficiency SAE. Results allow concluding that the injection of the air flow from the suction side of the pump promotes the generation of microbubbles (MB) for a maximum air flow allowed by the system. SOTR and SAE could be maximized whit the flow ratio factor and the Venturi-Vortex generator, supplying air flow from the suction side of the hydraulic pump.

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This study evaluated carbon dynamics in a carbonate microbasin (Bule stream), located in an important metallogenetic area (QF) in Brazil. River water collections were performed on a base and high flow. The microbasin showed high DIC, with HCO3- being the main component, followed by CO2. The pCO2 showed high values, indicating fluxes in the water → air direction. The degassing was 683.4 mmol m-2 day-1 (average at low flow) and 43.4 mmol m-2 day-1 (average at high flow). The fluvial flow was 9 to 31 kg km-2 day-1 of HCO3- and 1.4 to 0.7 kg km-2 day-1 of CO2, respectively, at the base and high flow. Considering the importance of the lithological unit of the Minas Supergroup for the QF, the total flow of CO2 released by all the basins that compose such units was estimated. The estimated values were 1.3 × 1012 mmol day-1 in low flow and 8.4 × 1010 mmol day-1 in high flow. The data obtained in this study were the first in the QF region. The Bule stream is an environmental protection area, free from contamination, considered a background for other drainage basins inserted in the QF. The results obtained reinforce the importance of hydrographic microbasins influence on the river carbon fluxes in a regional and global context.

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Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitute for microbiological Luria Broth (LB) medium for the growth of B. circulans E9 and the production of IAA. In Erlenmeyer flasks with pea fluor medium (PYM), the maximum production of IAA was 7.81 ± 0.16 µg mL-1, while in microbiological LB medium, it was 3.73 ± 0.15 µg mL-1. In addition, an oxygen transfer rate (OTR) of 1.04 kg O2 m-3 d-1 allowed the highest bacterial growth (19.3 ± 2.18 × 1010 CFU mL-1) and IAA production (10.7 µg mL-1). Consequently, the OTR value from the flask experiments was used to define the conditions for the operation of a 1 L stirred tank bioreactor. The growth and IAA production of B. circulans cultured in a bioreactor with PYM medium were higher (8 and 1.6 times, respectively) than those of bacteria cultured in Erlenmeyer flasks. IAA produced in a bioreactor by B. circulans was shown to induce the root system in Arabidopsis thaliana, similar to synthetic IAA. The results of this study demonstrate that PYM medium may be able to be used for the mass production of B. circulans E9 in bioreactors, increasing both bacterial growth and IAA production. This low-cost medium has the potential to be employed to grow other IAA-producing bacterial species.

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The assessment of heat transfer is a complex task, especially for operations in the oil and gas industry, due to the harsh and flammable workspace. In light of the limitations of conventional sensors in harsh environments, this paper presents a fiber Bragg grating (FBG)-based sensor for the assessment of the heat transfer rate (HTR) in different liquids. To better understand the phenomenon of heat distribution, a preliminary analysis is performed by constructing two similar scenarios: those with and without the thermal insulation of a styrofoam box. The results indicate the need for a minimum of thermal power to balance the generated heat with the thermal losses of the setup. In this minimum heat, the behavior of the thermal distribution changes from quadratic to linear. To assess such features, the estimation of the specific heat capacity and the thermal conductivity of water are performed from 3 W to 12 W, in 3 W steps, resulting in a specific heat of 1.144 cal/g °C and thermal conductivity of 0.5682 W/m °C. The calibration and validation of the HTR sensor is performed in a thermostatic bath. The method, based on the temperature slope relative to the time curve, allowed for the measurement of HTR in water and Kryo 51 oil, for different heat insertion configurations. For water, the HTR estimation was 308.782 W, which means an uncertainty of 2.8% with the reference value of the cooling power (300 W). In Kryo 51 oil, the estimated heat absorbed by the oil was 4.38 kW in heating and 718.14 kW in cooling.

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This paper presents the implementation of nonlinear canonical correlation analysis (NLCCA) approach to detect steady-state visual evoked potentials (SSVEP) quickly. The need for the fast recognition of proper stimulus to help end an SSVEP task in a BCI system is justified due to the flickering external stimulus exposure that causes users to start to feel fatigued. Measuring the accuracy and exposure time can be carried out through the information transfer rate-ITR, which is defined as a relationship between the precision, the number of stimuli, and the required time to obtain a result. NLCCA performance was evaluated by comparing it with two other approaches-the well-known canonical correlation analysis (CCA) and the least absolute reduction and selection operator (LASSO), both commonly used to solve the SSVEP paradigm. First, the best average ITR value was found from a dataset comprising ten healthy users with an average age of 28, where an exposure time of one second was obtained. In addition, the time sliding window responses were observed immediately after and around 200 ms after the flickering exposure to obtain the phase effects through the coefficient of variation (CV), where NLCCA obtained the lowest value. Finally, in order to obtain statistical significance to demonstrate that all approaches differ, the accuracy and ITR from the time sliding window responses was compared using a statistical analysis of variance per approach to identify differences between them using Tukey's test.

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BACKGROUND: Alginates are polysaccharides used in a wide range of industrial applications, with their functional properties depending on their molecular weight. In this study, alginate production and the expression of genes involved in polymerization and depolymerization in batch cultures of Azotobacter vinelandii were evaluated under controlled and noncontrolled oxygen transfer rate (OTR) conditions. RESULTS: Using an oxygen transfer rate (OTR) control system, a constant OTR (20.3 ± 1.3 mmol L 1 h 1 ) was maintained during cell growth and stationary phases. In cultures subjected to a controlled OTR, alginate concentrations were higher (5.5 ± 0.2 g L 1 ) than in cultures under noncontrolled OTR. The molecular weight of alginate decreased from 475 to 325 kDa at the beginning of the growth phase and remained constant until the end of the cultivation period. The expression level of alyA1, which encodes an alginate lyase, was more affected by OTR control than those of other genes involved in alginate biosynthesis. The decrease in alginate molecular weight can be explained by a higher relative expression level of alyA1 under the controlled OTR condition. CONCLUSIONS: This report describes the first time that alginate production and alginate lyase (alyA1) expression levels have been evaluated in A. vinelandii cultures subjected to a controlled OTR. The results show that automatic control of OTR may be a suitable strategy for improving alginate production while maintaining a constant molecular weight.

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Alginates can be used to elaborate hydrogels, and their properties depend on the molecular weight (MW) and the guluronic (G) and mannuronic (M) composition. In this study, the MW and G/M ratio were evaluated in cultures of Azotobacter vinelandii to 3 and 30 L scales at different oxygen transfer rates (OTRs) under diazotrophic conditions. An increase in the maximum OTR (OTRmax) improved the alginate production, reaching 3.3 ± 0.2 g L-1. In the cultures conducted to an OTR of 10.4 mmol L-1 h-1 (500 rpm), the G/M increased during the cell growth phase and decreased during the stationary phase; whereas, in the cultures at 19.2 mmol L-1 h-1 was constant throughout the cultivation. A higher alginate MW (520 ± 43 kDa) and G/M ratio (0.86 ± 0.01) were obtained in the cultures conducted at 10.4 mmol L-1 h-1. The OTR as a criterion to scale up alginate production allowed to replicate the concentration and the alginate production rate; however, it was not possible reproduce the MW and G/M ratio. Under a similar specific oxygen uptake rate (qO2) (approximately 65 mmol g-1 h-1) the alginate MW was similar (approximately 365 kDa) in both scales. The evidences revealed that the qO2 can be a parameter adequate to produce alginate MW similar in two bioreactor scales. Overall, the results have shown that the alginate composition could be affected by cellular respiration, and from a technological perspective the evidences contribute to the design process based on oxygen consumption to produce alginates defined.

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Azotobacter vinelandii produces the linear exopolysaccharide alginate, a compound of significant biotechnological importance. The biosynthesis of alginate in A. vinelandii and Pseudomonas aeruginosa has several similarities but is regulated somewhat differently in the two microbes. Here, we show that the second messenger cyclic dimeric GMP (c-di-GMP) regulates the production and the molecular mass of alginate in A. vinelandii The hybrid protein MucG, containing conserved GGDEF and EAL domains and N-terminal HAMP and PAS domains, behaved as a c-di-GMP phosphodiesterase (PDE). This activity was found to negatively affect the amount and molecular mass of the polysaccharide formed. On the other hand, among the diguanylate cyclases (DGCs) present in A. vinelandii, AvGReg, a globin-coupled sensor (GCS) DGC that directly binds to oxygen, was identified as the main c-di-GMP-synthesizing contributor to alginate production. Overproduction of AvGReg in the parental strain phenocopied a ΔmucG strain with regard to alginate production and the molecular mass of the polymer. MucG was previously shown to prevent the synthesis of high-molecular-mass alginates in response to reduced oxygen transfer rates (OTRs). In this work, we show that cultures exposed to reduced OTRs accumulated higher levels of c-di-GMP; this finding strongly suggests that at least one of the molecular mechanisms involved in modulation of alginate production and molecular mass by oxygen depends on a c-di-GMP signaling module that includes the PAS domain-containing PDE MucG and the GCS DGC AvGReg.IMPORTANCE c-di-GMP has been widely recognized for its essential role in the production of exopolysaccharides in bacteria, such as alginate produced by Pseudomonas and Azotobacter spp. This study reveals that the levels of c-di-GMP also affect the physical properties of alginate, favoring the production of high-molecular-mass alginates in response to lower OTRs. This finding opens up new alternatives for the design of tailor-made alginates for biotechnological applications.

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A new method for CO2 recovery was proposed for cultivation of different microalgae. First, a chemical fixation, where CO2 was injected in alkalinized vinasse to form (bi)carbonate salts, was performed. In addition, biological fixation with CO2-enriched air injection was also accomplished for evaluation of the most promising results. Two bioreactor systems, a stirred-tank reactor and a bubble column reactor, were employed. A higher carbon transfer rate (43.35 g.L-1.h-1) was achieved in the bubble column reactor using NaOH-alkalinized vinasse, along with reductions of the chemical oxygen demand (COD), biological oxygen demand (BOD) and turbidity (TD). This allowed the cultivation of microalgae and cyanobacteria at vinasse concentrations between 70 and 100%, reaching a biomass production of 2.25 g.L-1 in 15 days of culture. The viability of chemical CO2 fixation together with the use of 100% treated vinasse from a bioethanol production unit for microalgae cultivation has been demonstrated in a successfully integrated biorefinery approach.

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Charge hopping based on Marcus theory is often used to predict charge carrier mobilities in organic crystals, although it is known to systematically underestimate the values. Here we show that this deficiency may lie on a fundamental aspect of quantum statistical averages, rather than on the approximation itself. Under adequate Boltzmann weighing procedure used to evaluate electron and hole transfer integrals, a kinetic Monte Carlo model is employed to describe mobilities in an azacene derivative. The values are in good agreement with experimental data suggesting that the evaluation of transfer integrals may be the weak link in hopping transport models.

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BACKGROUND: In Streptomyces, understanding the switch from primary to secondary metabolism is important for maximizing the production of secondary metabolites such as antibiotics, as well as for optimizing recombinant glycoprotein production. Differences in Streptomyces lividans bacterial aggregation as well as recombinant glycoprotein production and O-mannosylation have been reported due to modifications in the shake flask design. We hypothetized that such differences are related to the metabolic switch that occurs under oxygen-limiting conditions in the cultures. RESULTS: Shake flask design was found to affect undecylprodigiosin (RED, a marker of secondary metabolism) production; the RED yield was 12 and 385 times greater in conventional normal Erlenmeyer flasks (NF) than in baffled flasks (BF) and coiled flasks (CF), respectively. In addition, oxygen transfer rates (OTR) and carbon dioxide transfer rates were almost 15 times greater in cultures in CF and BF as compared with those in NF. Based on these data, we obtained respiration quotients (RQ) consistent with aerobic metabolism for CF and BF, but an RQ suggestive of anaerobic metabolism for NF. CONCLUSION: Although the metabolic switch is usually related to limitations in phosphate and nitrogen in Streptomyces sp., our results reveal that it can also be activated by low OTR, dramatically affecting recombinant glycoprotein production and O-mannosylation and increasing RED synthesis in the process.

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Este estudo teve como objetivo investigar a tolerância ou susceptibilidade das espécies de capim-colchão (Digitaria ciliaris, D. horizontalis e D. nuda) a herbicidas inibidores do fotossistema II (FSII), por meio da técnica da fluorescência, utilizando a taxa de transferência de elétrons do FSII como indicador. Para isso, foi conduzido um experimento em esquema fatorial 3x6, com três espécies de capim-colchão e seis tratamentos (ametryn, hexazinone, diuron+hexazinone, amicarbazone, diuron, tebuthiuron e testemunha) aplicados em pós-emergência. Foi monitorada a taxa de transporte de elétrons (ETR) do FSII em intervalos de tempo crescente e aferida a massa seca das plantas aos 21 dias após a aplicação. A partir dos dados da ETR, calculou-se um valor numérico representativo da taxa de inativação da ETR. Os resultados demonstraram que todas as espécies de capim-colchão estudadas são susceptíveis aos herbicidas ametryn e hexazinone (valores da taxa de inativação da ETR superiores a 10.000). Os herbicidas diuron e tebuthiuron apresentaram menores taxas de inativação da ETR para a D. nuda (3.585 e 3.497, respectivamente) e maiores para as espécies D. ciliaris e D. horizontalis (acima de 10.000), enquanto que o herbicida amicarbazone apresentou valor intermediário para D. nuda (7.967). O monitoramento da ETR foi eficiente para verificar a atuação dos herbicidas nas diferentes espécies estudadas.

This study aimed to investigate the tolerance or susceptibility of crabgrass species (Digitaria ciliaris, D. horizontalis and D. nuda) to herbicides that target photosystem II (PSII), by fluorescence technique using the electron transport rate (ETR) through PSII as an indicator. An experiment was conducted under a factorial arrange (3x6), with three species of crabgrass and six treatments (ametryn, hexazinone, diuron+hexazinone, amicarbazone, diuron, tebuthiuron and control) applied in post-emergence. The ETR through PSII was monitored over time and the plants dry weight measured at 21 days after application. A numerical value of the ETR inactivation was calculated from the data collected. The results showed that the three crabgrass species studied are susceptible to ametryn and hexazinone (ETR inactivation value higher than 10,000). Moreover, diuron and tebuthiuron provided lower ETR inactivation for D. nuda (3,585 and 3,497, respectively) and higher rates for D. horizontalis and D. ciliaris (more than 10,000), whereas amicarbazone showed intermediate inactivation rate for D. nuda (7,967). Monitoring the ETR showed to be an efficient form to verify the herbicides performance in the different species studied here.

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Este estudo teve como objetivo investigar a tolerância ou susceptibilidade das espécies de capim-colchão (Digitaria ciliaris, D. horizontalis e D. nuda) a herbicidas inibidores do fotossistema II (FSII), por meio da técnica da fluorescência, utilizando a taxa de transferência de elétrons do FSII como indicador. Para isso, foi conduzido um experimento em esquema fatorial 3x6, com três espécies de capim-colchão e seis tratamentos (ametryn, hexazinone, diuron+hexazinone, amicarbazone, diuron, tebuthiuron e testemunha) aplicados em pós-emergência. Foi monitorada a taxa de transporte de elétrons (ETR) do FSII em intervalos de tempo crescente e aferida a massa seca das plantas aos 21 dias após a aplicação. A partir dos dados da ETR, calculou-se um valor numérico representativo da taxa de inativação da ETR. Os resultados demonstraram que todas as espécies de capim-colchão estudadas são susceptíveis aos herbicidas ametryn e hexazinone (valores da taxa de inativação da ETR superiores a 10.000). Os herbicidas diuron e tebuthiuron apresentaram menores taxas de inativação da ETR para a D. nuda (3.585 e 3.497, respectivamente) e maiores para as espécies D. ciliaris e D. horizontalis (acima de 10.000), enquanto que o herbicida amicarbazone apresentou valor intermediário para D. nuda (7.967). O monitoramento da ETR foi eficiente para verificar a atuação dos herbicidas nas diferentes espécies estudadas.(AU)

This study aimed to investigate the tolerance or susceptibility of crabgrass species (Digitaria ciliaris, D. horizontalis and D. nuda) to herbicides that target photosystem II (PSII), by fluorescence technique using the electron transport rate (ETR) through PSII as an indicator. An experiment was conducted under a factorial arrange (3x6), with three species of crabgrass and six treatments (ametryn, hexazinone, diuron+hexazinone, amicarbazone, diuron, tebuthiuron and control) applied in post-emergence. The ETR through PSII was monitored over time and the plants dry weight measured at 21 days after application. A numerical value of the ETR inactivation was calculated from the data collected. The results showed that the three crabgrass species studied are susceptible to ametryn and hexazinone (ETR inactivation value higher than 10,000). Moreover, diuron and tebuthiuron provided lower ETR inactivation for D. nuda (3,585 and 3,497, respectively) and higher rates for D. horizontalis and D. ciliaris (more than 10,000), whereas amicarbazone showed intermediate inactivation rate for D. nuda (7,967). Monitoring the ETR showed to be an efficient form to verify the herbicides performance in the different species studied here.(AU)

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