RESUMO
MicroRNAs (miRNAs) are non-coding RNAs involved in the regulation of gene expression associated with cell differentiation, proliferation, adhesion, and important biological functions such as inflammation. miRNAs play roles associated with the pathogenesis of chronic degenerative disorders including cardiovascular diseases. Understanding the influence of miRNAs and their target genes can effectively streamline the identification of key biologically active pathways that are important in the development of vascular grafts through the tissue engineering of blood vessels. To determine miRNA expression levels and identify miRNA target genes and pathways with biological roles in scaffolds that have been repopulated with adipose-derived stem cells (ASCs) generated through tissue engineering for the construction of blood vessels. miRNA quantification assays were performed in triplicate to determine miRNA expression in a total of 20 samples: five controls (natural inferior vena cava), five scaffolds recellularized with ASCs and differentiated into the endothelium (luminal layer), five samples of complete scaffolds seeded with ASCs differentiated into the endothelium (luminal layer) and smooth muscle (extraluminal layer), and five samples of ASC without cell differentiation. Several differentially expressed miRNAs were identified and predicted to modulate target genes with roles in key pathways associated with angiogenesis, vascular system control, and endothelial and smooth muscle regulation, including migration, proliferation, and growth. These findings underscore the involvement of these pathways in the regulatory mechanisms that are essential for vascular scaffold production through tissue engineering. Our research contributes to the knowledge of miRNA-regulated mechanisms, which may impact the design of vascular substitutes, and provide valuable insights for enhancing clinical practice. The molecular pathways regulated by miRNAs in tissue engineering of blood vessels (TEBV) allowed us to elucidate the main phenomena involved in cellular differentiation to constitute a blood vessel, with the main pathways being essential for angiogenesis, cellular differentiation, and differentiation into vascular smooth muscle.
Assuntos
Diferenciação Celular , MicroRNAs , Engenharia Tecidual , Alicerces Teciduais , MicroRNAs/genética , MicroRNAs/metabolismo , Engenharia Tecidual/métodos , Humanos , Alicerces Teciduais/química , Diferenciação Celular/genética , Tecido Adiposo/metabolismo , Tecido Adiposo/citologia , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/crescimento & desenvolvimento , Regulação da Expressão Gênica , Neovascularização Fisiológica/genética , Células-Tronco/metabolismo , Células-Tronco/citologia , Proliferação de Células/genética , Transdução de SinaisRESUMO
BACKGROUND: The standard clinical treatment for hypoparathyroidism, replacement of calcium and vitamin metabolites (calcitriol), has been used for decades; however, evidence points to its inefficiency in acting on the pathophysiology of the disease, which may precipitate or aggravate conditions already related to hypoparathyroidism. Therapies based on recombinant human parathyroid hormone have emerged in recent years but still have low availability due to their high cost. Parathyroid allotransplantation (Pt-a) has been reported as a strategy for treating more severe cases. METHODS: This narrative review highlights relevant aspects of conventional permanent hypoparathyroidism treatment and provides a comprehensive and critical review of the reports of applications of Pt-a, especially those carried out in recent years. Particular focus is placed on the following key points: parathyroid immunogenicity, immunosuppression regimens (short-term or chronic), techniques to reduce the expression of immunogenic molecules, follow-up time, and reductions in calcium and vitamin D supplementation. CONCLUSION: Pt-a has been considered a safe and relatively low-cost therapy and is believed to have the potential to cure the disease, in addition to treating symptoms. However, there is considerable heterogeneity in treatment protocols; therefore, more studies are required to improve the standardization of the procedure and thus improve the consistency of outcomes.
Assuntos
Hipocalcemia , Hipoparatireoidismo , Humanos , Cálcio/uso terapêutico , Hipocalcemia/tratamento farmacológico , Hormônio Paratireóideo/uso terapêutico , Hipoparatireoidismo/tratamento farmacológico , Hipoparatireoidismo/diagnóstico , Glândulas Paratireoides/cirurgia , Calcitriol/uso terapêuticoRESUMO
Introdução: A enxertia homóloga de pele pode representar a diferença entre a vida e a morte de pacientes grandes queimados. Sua utilização consiste em um tratamento precioso quando não há a disponibilidade do enxerto autólogo. Os bancos de tecidos foram criados para realizar o processamento e armazenamento da pele alógena. O objetivo é analisar o perfil epidemiológico de doadores e receptores de pele do banco de pele do Hospital Universitário Evangélico Mackenzie (HUEM) e a sua produtividade, desde a inauguração em 2013 até 2019. Métodos: Consulta aos relatórios anuais do período de sete anos. Resultados: Captou-se a pele de 187 doadores, dos quais 61% eram homens e 39%, mulheres. A idade média foi de 41,07 anos. O número de doadores atingiu a média de 21,3 por ano. Foram coletados, no total, 201.000cm2 de tecido viável, que resultaram na produção de 3.770 lâminas de pele. Desde 2013, foram realizados no HUEM, 325 enxertos alógenos que beneficiaram 194 pessoas. A idade média dos pacientes que receberam a pele foi de 34,67 anos. A maior parte da pele captada, processada e armazenada pelo banco do HUEM (cerca 91%) foi utilizada em enxertias realizadas na própria instituição. Conclusão: O banco de pele do HUEM disponibilizou aloenxertos que beneficiaram 194 pessoas em 7 anos de funcionamento. Em sua maioria, os doadores e receptores eram do sexo masculino e tinham, aproximadamente, 40 anos de idade. O número de captações realizadas por este banco de pele foi compatível com o de outras instituições do Brasil
Introduction: Homologous skin grafting may represent the difference between the life and death of large burned patients. Its use consists of a precious treatment when there is no availability of autologous graft. The tissue banks were created to perform the processing and storage of the allogenous skin. The objective is to analyze the epidemiological profile of skin donors and recipients from the skin bank of the Hospital Universitário Evangélico Mackenzie (HUEM) and its productivity, from its inauguration in 2013 to 2019. Methods: Consultation of annual reports for the seven years. Results: The skin of 187 donors was captured, of which 61% were men and 39% were women. The mean age was 41.07 years. The number of donors averaged 21.3 per year. A total of 201,000cm2 of viable tissue were collected, which resulted in 3,770 skin slides. Since 2013, 325 allogenous grafts have been performed at HUEM that have benefited 194 people. The mean age of patients receiving the skin was 34.67 years. Most of the skin captured, processed, and stored by the HUEM bank (about 91%) was used in grafting carried out in the institution itself. Conclusion: The HUEM skin bank provided allografts that benefited 194 people in 7 years of operation. Most donors and recipients were male and approximately 40 years old. The number of captures performed by this skin bank was compatible with that of other institutions in Brazil.
RESUMO
The transplantation of tissues can save lives and re-establish vital functions, where no alternatives of comparable effectiveness exist. This has led to establishment of tissue transplantation as a successful practice worldwide; however, a great variability between countries remains in terms of donation levels, safety, quality of grafts and their efficacy. Tissue transplantation requires coordination of different agencies involved in the implementation of procurement, processing, storage and distribution of tissues and cells from different hospital units that perform surgical procedures with graft-type input requirements. This biomaterial-like requirement has led to the constant development of the area and today these graft products of human origin can be the starting point for new and more advanced biotechnological products. For long-term sustainability and successful transplantation units, a process management comparable to the pharmaceutical industry in terms of quality management systems must be established to produce safe and high-quality human-derived products. This review aims to update the current concepts of tissue transplant services for its application for developing countries using the current Chilean scenario as a case study. We summarize our findings proposing a set of guidelines/actions that should be followed to ensure smooth tissue transplant services implementations with high efficiency and safe use.
Assuntos
Obtenção de Tecidos e Órgãos , Transplantes , Países em Desenvolvimento , HumanosRESUMO
RESUMEN Introducción: Los objetivos de la reconstrucción auricular son mantener la permeabilidad del canal auditivo externo y restaurar la forma general y la proyección. Objetivo: caracterizar un caso clínico de reconstrucción postraumática de una pérdida parcial auricular, enfatizando en la importancia de la secuencia terapéutica empleada. Presentación del caso clínico: Acude a consulta externa un paciente masculino de 26 años. Refiere haber sido tratado en el cuerpo de guardia 10 días atrás, al haber perdido un fragmento de oreja por una mordida en una riña. Al examen físico se constata la existencia de una pérdida parcial irregular de la oreja derecha. Se decide esperar tres semanas, e indicar chequeo preoperatorio. Al cabo de este tiempo se realiza el primer tiempo reconstructivo quirúrgico, con injerto autólogo de cartílago auricular de la oreja izquierda, que fue colocado en forma de "bolsillo" en la región temporal. A las tres semanas del primer tiempo quirúrgico, se realiza, bajo anestesia local, el segundo tiempo reconstructivo con la liberación de la región auricular del "bolsillo" creado, el avance de un colgajo posterior y la colocación de un injerto libre de piel. El paciente refirió una gran satisfacción por los resultados estéticos obtenidos. Principales comentarios: se empleó una secuencia terapéutica ante un defecto traumático parcial del pabellón auricular en sus tercios superior y medio. Este tipo de secuela traumática requirió el empleo de procederes quirúrgicos en varios tiempos operatorios que garantizaran un buen aporte sanguíneo de los tejidos, ausencia de tensión y adecuados resultados estéticos finales(AU)
ABSTRACT Introduction: The aims of auricular reconstruction are to maintain the permeability of the external auditory canal and restore general shape and projection. Objective: Characterize a clinical case of posttraumatic reconstruction of a partial ear loss, highlighting the importance of the therapy sequence followed. Clinical case presentation: A male 26-year-old patient attends outpatient consultation. He reports that he was treated in the emergency service 10 days before upon having lost a section of his ear due to a bite in a fight. Physical examination reveals irregular partial loss of the right ear. It is decided to wait three weeks and indicate preoperative checkup. At the end of that period the first surgical reconstruction session is conducted, with autologous graft of auricular cartilage from the left ear, which was placed in the form of a "pocket" in the temporal region. Three weeks after the first surgical session, the second reconstruction is performed under local anesthesia, with release of the "pocket" from the auricular region, advancement of a posterior flap and placement of a free skin graft. The patient reported great satisfaction with the esthetic results obtained. Main remarks: A therapy sequence was followed in response to a partial traumatic defect of the outer ear in its upper and middle thirds. This type of traumatic sequel required the performance of surgical procedures at several operative moments, ensuring good blood supply to tissues, absence of tension and appropriate final esthetic results(AU)
Assuntos
Humanos , Masculino , Adulto , Retalhos Cirúrgicos/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Cartilagem da Orelha/transplante , Pavilhão Auricular/lesõesRESUMO
Long-term dietary and pharmacological treatments for obesity have been questioned, particularly in individuals with severe obesity, so a new approach may involve adipose tissue transplants, particularly autologous transplants. Thus, the aim of this study was to evaluate the metabolic effects of autologous subcutaneous adipose tissue (SAT) transplants into two specific intraabdominal cavity sites (omental and retroperitoneal) after 90 days. The study was performed using two different diet-induced obesity (DIO) rat models: one using a high-fat diet (HFD) and the other using a high-carbohydrate diet (HCHD). Autologous SAT transplant reduced hypertrophic adipocytes, improved insulin sensitivity, reduced hepatic lipid content, and fasting serum-free fatty acids (FFAs) concentrations in the two DIO models. In addition, the reductions in FFAs and glycerol were accompanied by a greater reduction in lipolysis, assessed via the phosphorylation status of HSL, in the transplanted adipose tissue localized in the omentum compared with that localized in the retroperitoneal compartment. Therefore, the improvement in hepatic lipid content after autologous SAT transplant may be partially attributed to a reduction in lipolysis in the transplanted adipose tissue in the omentum due to the direct drainage of FFAs into the liver. The HCHD resulted in elevated fasting and postprandial serum insulin levels, which were dramatically reduced by the autologous SAT transplant. In conclusion, the specific intraabdominal localization of the autologous SAT transplant improved the carbohydrate and lipid metabolism of adipose tissue in obese rats and selectively corrected the metabolic parameters that are dependent on the type of diet used to generate the DIO model.