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Food bioactive peptides are well recognized for their health benefits such as antimicrobial, antioxidant, and antihypertensive benefits, among others. Their drug-like behavior has led to their potential use in targeting skin-related aging factors like the inhibition of enzymes related with the skin-aging process. In this study, canary seed peptides (CSP) after simulated gastrointestinal digestion (<3 kDa) were fractioned by RP-HPLC and their enzyme-inhibition activity towards elastase and tyrosinase was evaluated in vitro. CSP inhibited elastase (IC50 = 6.2 mg/mL) and tyrosinase (IC50 = 6.1 mg/mL), while the hydrophobic fraction-VI (0.2 mg/mL) showed the highest inhibition towards elastase (93%) and tyrosinase (67%). The peptide fraction with the highest inhibition was further characterized by a multilevel in silico workflow, including physicochemical descriptor calculations, antioxidant activity predictions, and molecular dynamics-ensemble docking towards elastase and tyrosinase. To gain insights into the skin permeation process during molecular dynamics simulations, based on their docking scores, five peptides (GGWH, VPPH, EGLEPNHRVE, FLPH, and RPVNKYTPPQ) were identified to have favorable intermolecular interactions, such as hydrogen bonding of polar residues (W, H, and K) to lipid polar groups and 2-3 Å van der Waals close contact of hydrophobic aliphatic residues (P, V, and L). These interactions can play a critical role for the passive insertion of peptides into stratum corneum model skin-membranes, suggesting a promising application of CSP for skin-aging treatments.
Assuntos
Monofenol Mono-Oxigenase , Phalaris , Simulação de Dinâmica Molecular , Elastase Pancreática , Sementes , Antioxidantes/farmacologiaRESUMO
ABSTRACT Objective: To identify the frequency and factors associated to incontinence associated dermatitis in elderly people. Methods: Cross-sectional study with 202 elderly patients admitted to a university hospital between September 2017 and January 2018. Data collection included: cognitive screening, sociodemographic and clinical questionnaire. It was performed exploratory and descriptive analysis, where prevalence and ratios (PR) were calculated. Results: Prevalence of injury was 9.4%, age range 70-79 years (13.5%), African American (21.4%), hospitalization period ≥ 21 days (44.4%), in use of nasogastric tube (33.3%), medical device (11.3%), restricted mobility (18.5%). It was associated with hospitalization period, use of nasogastric tube, restricted mobility, medical devices, and cognition. Conclusion: It was confirmed an average frequency of incontinence associated dermatitis in elderly patients and association to factors such as hospitalization period, immobility, cognition, use of nasogastric tube and devices. It must be highlighted the importance of prevention measures, early detection, assessment and monitoring of this type of injury.
RESUMEN Objetivo: Identificar la prevalencia y los factores relacionados a la dermatitis relacionada a la incontinencia en ancianos. Métodos: Estudio transversal, con 202 ancianos internados en un hospital universitario, entre septiembre de 2017 a enero de 2018. La recogida de datos contempló: rastreo cognitivo, cuestionario sociodemográfico y clínico. Se realizó análisis exploratorio y descriptivo, siendo calculadas las prevalencias y razones de prevalencia (RP). Resultados: Prevalencia de la lesión ha sido de 9,4%, franja etaria 70-79 años (13,5%), raza negra (21,4%), tiempo de internación de ≥ 21 días (44,4%), en uso de sonda nasoenteral (33,3%), dispositivo médico (11,3%), movilidad restricta (18,5%). Se presentó relacionada con el tiempo de internación, sonda nasoenteral, movilidad restricta, dispositivos médicos y cognición. Conclusión: Se verificó la media prevalencia de dermatitis relacionada a la incontinencia en ancianos internados y relación a los factores: tiempo de internación, inmovilidad, cognición, uso de sonda nasoenteral y dispositivos. Se destaca la importancia de acciones de prevención, identificación precoz, evaluación y monitoreo de ese tipo de lesión.
RESUMO Objetivo: Identificar a prevalência e os fatores associados à dermatite associada à incontinência em idosos. Métodos: Estudo transversal, com 202 idosos internados em um hospital universitário, entre setembro de 2017 a janeiro de 2018. A coleta de dados contemplou: rastreio cognitivo, questionário sociodemográfico e clínico. Realizou-se análise exploratória e descritiva, sendo calculadas as prevalências e razões de prevalência (RP). Resultados: Prevalência da lesão foi de 9,4%, faixa etária 70-79 anos (13,5%), raça negra (21,4%), tempo de internação de ≥ 21 dias (44,4%), em uso de sonda nasoenteral (33,3%), dispositivo médico (11,3%), mobilidade restrita (18,5%). Apresentou-se associada com o tempo de internação, sonda nasoenteral, mobilidade restrita, dispositivos médicos e cognição. Conclusão: Verificou-se a média prevalência de dermatite associada à incontinência em idosos internados e associação aos fatores: tempo de internação, imobilidade, cognição, uso de sonda nasoenteral e dispositivos. Destaca-se a importância de ações de prevenção, identificação precoce, avaliação e monitoramento desse tipo de lesão.
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OBJECTIVE: High-frequency ultrasound is a non-invasive tool used in skin ageing research to assess dermis thickness and echogenicity. This study evaluated the reliability of a range of high-frequency ultrasound parameters and tested their correlation with age and a validated clinical scale for the assessment of forearm skin photoageing; the difference between two body sites according to environmental exposition patterns was also investigated. METHODS: Twenty-three volunteers aged 28-82 years were divided into three groups according to forearm photoageing degree. A 20 MHz ultrasound unit was used to obtain cross-sectional images of the skin by two trained investigators on two different sites: the dorsal forearm (chronically photoexposed skin) and the proximal medial arm (non-photoexposed skin). Several echogenicity parameters were studied for each skin compartment: total dermis, upper dermis and lower dermis, and the ratio between upper and lower dermis. RESULTS: The intraclass correlation coefficient (for complete agreement) between investigators was higher for upper and total dermis echogenicity measures compared with the lower dermis. At the non-photoexposed site, the upper and lower dermis parameter ratio was better correlated with age. At the photoexposed area, total dermis parameters demonstrated higher correlations with clinical score. CONCLUSION: The authors discuss the choice of parameters for forearm photoageing assessment using high-frequency ultrasound.