RESUMO
The immobilization of TiO2-SiO2 (TSO) materials on seed mats stands as a practical way to help the germination and early growth of tomato plants (Solanum Lycopersicum). Mesoporous materials are functionalized with triethanolamine (TEA) and loaded with the biocide molecule of carvacrol (CAR). The effect of CAR on the parameters of germination percentage, germination time, root, shoot length, and chlorophyll content of seeds and/or tomato seedlings are investigated. The germination experiments were carried out using seed mats coated with the TSO materials, also TSO powdered materials were put directly on the tomato seeds to study their effect on germination. Direct deposition of TSO composites achieved the complete germination and longer shoots due to the cooperative interactions among nanomaterials, carvacrol, and the tomato seed. However, the handling of the seeds and the detrimental effect of powder in the germination system made difficult the application with agricultural purposes. The plastic seed mats provide a practical system with lower germination, but more homogenous growth of root/shoot is possible. Surprisingly, in this methodology the carvacrol presents a detrimental effect on germination due to less interaction with the seeds. The handling of seeds and recover of the nanomaterials and its reuse are advantages of the plastic seed mats, which together with less wastage of seeds suggest a potential use in agriculture. The as-synthetized TSO NPs, together with the functionalization of triethanolamine and carvacrol used to promote the health germination of the seeds, allows the control of the time for seed germination, germination %, and length for the root/shoot of seed tomato germination. The immobilization of mesoporous materials results in an alternative to help the germination and early growth of agricultural plants searching to avoid the lixiviation of nanomaterials to the environment.
Assuntos
Germinação , Solanum lycopersicum , Dióxido de Silício , SementesRESUMO
Agriculture needs to decrease the use of agrochemicals due to their high toxicity and adopt new strategies to achieve sustainable food production. Therefore, nanoparticles (NPs) and plant growth-promoting bacteria (PGPB) have been proposed as viable strategies to obtain better crop yields with less environmental impact. Here, we describe the effect of silica nanoparticles (SiO2-NPs) on survival, antioxidant enzymatic activity, phosphate solubilization capacity, and gibberellin production of Bacillus cereus-Amazcala (B.c-A). Moreover, the effect of the co-application of SiO2-NPs and B.c-A on seed germination, physiological characteristics, and antioxidant enzymatic activity of chili pepper plants was investigated under greenhouse conditions. The results indicated that SiO2-NPs at 100 ppm enhanced the role of B.c-A as PGPB by increasing its phosphate solubilization capacity and the production of GA7. Moreover, B.c-A catalase (CAT) and superoxide dismutase (SOD) activities were increased with SiO2-NPs 100 ppm treatment, indicating that SiO2-NPs act as a eustressor, inducing defense-related responses. The co-application of SiO2-NPs 100 ppm and B.c-A improved chili pepper growth. There was an increase in seed germination percentage, plant height, number of leaves, and number and yield of fruits. There was also an increase in CAT and PAL activities in chili pepper plants, indicating that bacteria-NP treatment induces plant immunity.
RESUMO
In recent years, much attention has been given to nanoparticles (NPs) due to their many possible applications, and as research has progressed, these NPs have become valuable tools for medical purposes. Among many different types of NPs, silica nanoparticles (SiO2NPs) have been specifically evaluated for medical purposes and have also been used in many different types of products. Although SiO2NPs have already been applied and are believed to be nontoxic, there is still a concern regarding possible adverse effects that may be triggered after SiO2NP exposure. Therefore, in the present study, we employed a recommended cell line (BALB/c 3T3) for the toxicity evaluation to investigate the cytotoxic effects of SiO2NPs produced by chemical synthesis at a laboratory scale. First, we employed OECD guideline 129 in order to evaluate cytotoxicity effects and also estimate the starting doses for acute oral systemic toxicity tests. We evaluated the cytotoxic effects of two types of SiO2NPs (nonfluorescent and fluorescent) and found that they were not significantly different (IC50 = 1986.39 ± 237 µg/mL and IC50 = 1861.13 ± 186.72 µg/mL, respectively). Then, we used the predicted LD50 of both types of SiO2NPs to suggest that they could be categorized as GHS category 4 substances. By ultrastructural evaluation, we found that SiO2NPs are internalized by 3 T3 cells and are located in vacuole-like structures with no other significant changes in cell structure. We also found that SiO2NPs lead to cell necrosis in a dose-dependent manner.