RESUMO

Here we describe a mass spectrometry-based proteomics workflow to discovery proteins differentially regulated in brains collected postmortem from mental, neurological, or substance abuse disorders (MNS) patients. One way to maximize protein detection is to carry out enrichment of cellular compartments such as the nucleus, mitochondria and cytosol. Subcellular fractionation improves proteome coverage and may shed light on the role of these organelles in the pathophysiology of MNS.

Assuntos

Encefalopatias/genética , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Proteômica/métodos , Encefalopatias/patologia , Núcleo Celular/genética , Núcleo Celular/patologia , Humanos , Proteoma/genética , Frações Subcelulares/patologia