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The Physalis genus is composed of species with economic and medicinal importance, its cultivation is expanding in Brazil, and is needed research on the performance of species in different regions of the country. Thus, the objective was to characterize the growth, development and yield of Physalis angulata and Physalis ixocarpa species cultivated in three sowing seasons in Bahia's semiarid region. The days after sowing were determined for the occurrence of vegetative and reproductive stages, and the length and diameter of the main branch were evaluated weekly. At the end of cultivation, the number and total weight of fruits per plant were determined. The plants sown in April showed the best results, with acceleration in flowering, higher productivity and growth close to other producing regions, which is the best period for the cultivation of species. P. angulata is a more tolerant species and can be sown at different periods without compromising yield. High temperatures combined with scarcity of rainfall compromise the development of plants of both species of Physalis.

O gênero Physalis é composto por espécies de importância econômica e medicinal e seu cultivo está em expansão no Brasil, sendo necessárias pesquisas sobre o desempenho de espécies em diferentes regiões do país. Assim, o objetivo desse trabalho é caracterizar o crescimento, desenvolvimento e produtividade das espécies de Physalis angulata e Physalis ixocarpa cultivadas em três épocas de semeadura na região semiárida da Bahia. Os números de dias após a semeadura foram determinados para a ocorrência dos estágios vegetativo e reprodutivo, e o comprimento e o diâmetro do ramo principal foram avaliados semanalmente. Ao final do cultivo, foram determinados o número e o peso total de frutos por planta. As plantas semeadas em abril apresentaram os melhores resultados, com aceleração da floração, maior produtividade e crescimento próximo a outras regiões produtoras, sendo o melhor período para o cultivo das espécies. P. angulata é uma espécie mais tolerante e pode ser semeada em diferentes períodos sem comprometer o rendimento. Altas temperaturas combinadas com a escassez de chuvas comprometem o desenvolvimento das plantas de ambas as espécies de Physalis.

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The Physalis genus is composed of species with economic and medicinal importance, its cultivation is expanding in Brazil, and is needed research on the performance of species in different regions of the country. Thus, the objective was to characterize the growth, development and yield of Physalis angulata and Physalis ixocarpa species cultivated in three sowing seasons in Bahia's semiarid region. The days after sowing were determined for the occurrence of vegetative and reproductive stages, and the length and diameter of the main branch were evaluated weekly. At the end of cultivation, the number and total weight of fruits per plant were determined. The plants sown in April showed the best results, with acceleration in flowering, higher productivity and growth close to other producing regions, which is the best period for the cultivation of species. P. angulata is a more tolerant species and can be sown at different periods without compromising yield. High temperatures combined with scarcity of rainfall compromise the development of plants of both species of Physalis.(AU)

O gênero Physalis é composto por espécies de importância econômica e medicinal e seu cultivo está em expansão no Brasil, sendo necessárias pesquisas sobre o desempenho de espécies em diferentes regiões do país. Assim, o objetivo desse trabalho é caracterizar o crescimento, desenvolvimento e produtividade das espécies de Physalis angulata e Physalis ixocarpa cultivadas em três épocas de semeadura na região semiárida da Bahia. Os números de dias após a semeadura foram determinados para a ocorrência dos estágios vegetativo e reprodutivo, e o comprimento e o diâmetro do ramo principal foram avaliados semanalmente. Ao final do cultivo, foram determinados o número e o peso total de frutos por planta. As plantas semeadas em abril apresentaram os melhores resultados, com aceleração da floração, maior produtividade e crescimento próximo a outras regiões produtoras, sendo o melhor período para o cultivo das espécies. P. angulata é uma espécie mais tolerante e pode ser semeada em diferentes períodos sem comprometer o rendimento. Altas temperaturas combinadas com a escassez de chuvas comprometem o desenvolvimento das plantas de ambas as espécies de Physalis.(AU)

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Physalins are seco-steroids with a variety of pharmacological activities already described. In this study the pharmacological properties of a standardized concentrated ethanolic extract from Physalis angulata (CEEPA), rich in physalins B, D, F and G, were studied in models of pain and inflammation in mice. Inflammatory mediators were measured by radioimmunoassay and Real-Time PCR in mice paws after the CFA stimuli. Systemic administration of CEEPA produced antinociceptive effect on the writhing test and formalin test. In the writhing test, physalins B, D, F and G showed that the antinociceptive effect of CEEPA is more potent than that of these purified compounds. In addition, CEEPA reduced the levels of TNF-α, IL-1ß, COX-2 and iNOS mRNA in the CFA-induced paw inflammation. Likewise, CEEPA decreased the TNF-α, IL-1ß and PGE2 paw levels. In conclusion, CEEPA induces antinociceptive and anti-inflammatory effects, with improved pharmacological potency relative to pure physalins, associated to modulation of cytokine and cyclooxygenase pathways.

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The present work aimed to analyze the mineral nutrition of Physalis angulata L. under stress by aluminum in the nutrient solution. The treatments consisted of five different concentrations of aluminum in the nutrient solution (0, 0.04, 0.08, 0.12, and 0.16 mmol L-1) in the AlCl3 form. The plants were exposed to Al for 30 days. Subsequently, nutritional and aluminum analyses were performed on plant tissue. The data were submitted to analysis of variance (p < 0.05), and, in case of significance, the regression study was performed as well as hierarchical cluster analysis (HCA) and principal component analysis (PCA) were used. The formation of four groups occurred, where we can observe the similarity and differences in the treatments between them. The separation of the treatments into groups reflected the heterogeneity of the treatments about the aluminum levels in the nutrient solution, evidencing its phytotoxicity level in Physalis angulata plants. Among the analyzed variables, P, K, Ca, Mg, Fe, Mo, and Zn were the most influential ones demonstrated by principal component analysis (PCA). The stress of 0.16 mmol L-1 of Al increased the phosphorus contents in the stems and roots and the potassium, copper, and molybdenum contents in all parts of the plants. In contrast, Al reduced the levels of calcium, magnesium, iron, and zinc in P. angulata plants. Iron being the micronutrient that showed the largest reduction, followed by zinc in the leaves. The highest levels of aluminum were found in the roots.

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The use of natural products by communities from the Colombian Caribbean region to treat health issues, together with biodiversity and geographical features, constitute a great scenery to develop new therapies based on ethnopharmacological heritage. Here, we investigated the anti-inflammatory potential of 10 commonly used plants in Colombian folk medicine, evaluating their effect on nitric oxide (NO) production by LPS-stimulated RAW 264.7 macrophages. The most active plant was evaluated in vivo using 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema, along with its effect on the production of pro-inflammatory mediators in vitro. The extract of Physalis angulata L. calyces showed the highest activity. This extract was fractionated and its dichloromethane fraction (DF) was the most active in vitro, inhibiting the production of NO, prostaglandin E2 (PGE2), interleukin (IL)-1ß, IL-6, tumor necrosis factor (TNF)-α and monocyte chemotactic protein (MCP)-1 (CCL2). In vivo, DF showed a significant inhibition of ear edema and myeloperoxidase (MPO) activity, with evident reduction of the leukocyte infiltration into tissue. Our results support the ethnopharmacological use of the selected plants in folk medicine. P. angulata dichloromethane fraction represents a promising source of pharmacological compounds with great potential therapeutic use to treat inflammatory illness.

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BACKGROUND: Topical corticosteroids have been the most commonly prescribed drugs to treat skin inflammation, but their uses can lead to several adverse effects. Nowadays, new pharmacological strategies have been evaluated to improve dermatologic efficacy and reduce adverse effects, including natural products. OBJECTIVES: The aim of this study was to evaluate and compare the effects of a plant sterol standardized supercritical CO2 phytopharmaceutical of Physalis angulata L. with hydrocortisone on the immune and inflammatory mediators, and skin repair components production. Moreover, we studied effects of both products on the skin microcirculation and temperature in a double-blind placebo-controlled clinical trial. METHODS: Both products were evaluated on the immune (IL-6, IL-10, INF-γ, TNF-α, and IL-1α), inflammatory (COX-2, LOX, PLA2 , PGE2 , LTB4 , histamine, and NF-κB), and repair components (TGF-ß, GM-CSF, collagen, and GAG) production on human keratinocytes and fibroblast in non-stimulated and LPS-stimulated conditions. Indeed, in a randomized double-blind placebo-controlled clinical trial, we evaluated the effects of the both creams on the skin microcirculation and temperature using laser Doppler and infrared thermometer, respectively. RESULTS: Physalis angulata acted on the skin, modulating immune status and inflammatory response producing corticoid-like effects, but different of hydrocortisone, increased skin repair factors. The effects of phytopharmaceutical cream in the clinical trial promoted a better reduction in skin microcirculation and temperature than hydrocortisone. CONCLUSIONS: Taken together, the results indicate that sterol standardized CO2 supercritical preparation of P angulata is a new and innovative phytopharmaceutical with multiple pharmacological effects potentially useful as human skin protective product, particularly against cutaneous inflammatory disorders.

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OBJECTIVE: In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. METHODS: L. infantum promastigotes were treated with 50 and 100 µg/mL AEPa for 72 h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. RESULTS: Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50) = 65.9 µg/mL; selectivity index (SI) = 22.1) and amastigotes (IC50 = 37.9 µg/mL; SI = 38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment (IC50 = 0.2 µg/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa (100 µg/mL) promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000 µg/mL). CONCLUSION: AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

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The aim of this study was to evaluate the changes in quality of fruits of two species of physalis (P. angulata and P. pubescens) harvested from family farmer orchards in different maturity stages, comparing with fully ripen fruits to those of the commercial species (P. peruviana) of similar maturity. The experiment was conducted in a completely randomized design, in five maturity stages for P. angulata and in four for P. pubescens. Data were submitted to variance analysis and means of the maturity stages compared by the Tukey test at 5 % probability. For the comparison of fully ripen fruits of P. peruviana with the two species produced in different locations were used six replications and the means compared by Dunnetts test at 5 % probability. The fruit diameter varied from 15.1 to 18.0 mm that classifies it as of caliber B. During maturation the color of the fruit evolved from green to totally yellow (P. angulata) and to yellow-brown with purplish features (P. pubescens). The soluble solids (SS) contents of the fruits of P. angulata were superior to those of P. pubescens and the commercial species. Comparing with commercial species, the SS/AT ratio was higher in locally produced fruits, indicating more palatable fruits. Fruits of P. angulata present favorable characteristics for fresh consumption, with potential for extensive cultivation and trade in family horticulture.(AU)

O objetivo deste estudo foi avaliar as mudanças na qualidade de frutos de duas espécies de fisális (P. angulata e P. pubescens) colhidas de hortas de agricultores familiares em diferentes estádios de maturação, comparando os frutos completamente maduros aos da espécie comercial (P. peruviana) de maturidade similar. O experimento foi conduzido em delineamento inteiramente casualizado, em cinco estádios de maturação para P. angulata e em quatro para P. pubescens. Os dados foram submetidos a análise de variância e as médias dos estádios de maturação comparadas pelo teste de Tukey a 5 % de probabilidade. Na comparação dos frutos maduros da P. peruviana com os das espécies colhidas de diferentes localidades, foram utilizadas seis repetições, com as médias comparadas pelo teste de Dunnett, a 5% de probabilidade. O diâmetro do fruto variou de 15,1 a 18,0 mm que o classifica como de calibre B. Durante a maturação a coloração do fruto evoluiu do verde para completamente amarela (P. angulata) e para amarelo pardo com traços arroxeados (P. pubescens). O teor de sólidos solúveis (SS) de frutos de P. angulata foi superior aos da P. pubescens e da espécie comercial. Comparando com a espécie comercial, a relação SS/AT foi superior em frutos das espécies localmente produzidas, indicando frutos mais palatáveis. Assim, os frutos de P. angulata apresentam características favoráveis para o consumo fresco, com potencial para amplo cultivo e comercialização na horticultura familiar.(AU)

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The aim of this study was to evaluate the changes in quality of fruits of two species of physalis (P. angulata and P. pubescens) harvested from family farmer orchards in different maturity stages, comparing with fully ripen fruits to those of the commercial species (P. peruviana) of similar maturity. The experiment was conducted in a completely randomized design, in five maturity stages for P. angulata and in four for P. pubescens. Data were submitted to variance analysis and means of the maturity stages compared by the Tukey test at 5 % probability. For the comparison of fully ripen fruits of P. peruviana with the two species produced in different locations were used six replications and the means compared by Dunnett’s test at 5 % probability. The fruit diameter varied from 15.1 to 18.0 mm that classifies it as of caliber B. During maturation the color of the fruit evolved from green to totally yellow (P. angulata) and to yellow-brown with purplish features (P. pubescens). The soluble solids (SS) contents of the fruits of P. angulata were superior to those of P. pubescens and the commercial species. Comparing with commercial species, the SS/AT ratio was higher in locally produced fruits, indicating more palatable fruits. Fruits of P. angulata present favorable characteristics for fresh consumption, with potential for extensive cultivation and trade in family horticulture.

O objetivo deste estudo foi avaliar as mudanças na qualidade de frutos de duas espécies de fisális (P. angulata e P. pubescens) colhidas de hortas de agricultores familiares em diferentes estádios de maturação, comparando os frutos completamente maduros aos da espécie comercial (P. peruviana) de maturidade similar. O experimento foi conduzido em delineamento inteiramente casualizado, em cinco estádios de maturação para P. angulata e em quatro para P. pubescens. Os dados foram submetidos a análise de variância e as médias dos estádios de maturação comparadas pelo teste de Tukey a 5 % de probabilidade. Na comparação dos frutos maduros da P. peruviana com os das espécies colhidas de diferentes localidades, foram utilizadas seis repetições, com as médias comparadas pelo teste de Dunnett, a 5% de probabilidade. O diâmetro do fruto variou de 15,1 a 18,0 mm que o classifica como de calibre B. Durante a maturação a coloração do fruto evoluiu do verde para completamente amarela (P. angulata) e para amarelo pardo com traços arroxeados (P. pubescens). O teor de sólidos solúveis (SS) de frutos de P. angulata foi superior aos da P. pubescens e da espécie comercial. Comparando com a espécie comercial, a relação SS/AT foi superior em frutos das espécies localmente produzidas, indicando frutos mais palatáveis. Assim, os frutos de P. angulata apresentam características favoráveis para o consumo fresco, com potencial para amplo cultivo e comercialização na horticultura familiar.

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Leishmaniasis are a neglected group of emerging diseases that have been found in 98 countries and are caused by protozoa of the genus Leishmania. The therapy for leishmaniasis causes several side effects and leads to drug-resistant strains. Natural products from plants have exhibited activities against Leishmania in various experimental models. Physalis angulata is a widely used plant in popular medicine, and in the literature it has well-documented leishmanicidal activity. However, its mechanism of action is still unknown. Thus, this study aims to evaluate the mechanism driving the leishmanicidal activity of an aqueous extract of P. angulata root (AEPa). AEPa was effective against both promastigotes and intracellular amastigote forms of Leishmania amazonensis. This effect was mediated by an increase of reactive oxygen species (ROS), but not of nitric oxide (NO). The increased production of ROS induces cell death by phenotypes seems by apoptosis cell death in Leishmania, but not autophagy or necrosis. In addition, morphological analysis of macrophages showed that AEPa induced a high number of cytoplasmic projections, increased the volume of cytoplasm and number of vacuoles, caused cytoskeleton alterations and resulted in high spreading ability. AEPa also promoted superoxide anion (O2(-)) production in both uninfected macrophages and those infected with Leishmania. Therefore, these results revealed that AEPa causes cell death by phenotypes seems by apoptosis cell death in L. amazonensis and modulates macrophage activation through morphofunctional alterations and O2(-) generation to induce Leishmania death.

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BACKGROUND: The current treatment of Chagas disease, endemic in Latin America and emerging in several countries, is limited by the frequent side effects and variable efficacy of benznidazole. Natural products are an important source for the search for new drugs. AIM/HYPOTHESIS: Considering the great potential of natural products as antiparasitic agents, we investigated the anti-Trypanosoma cruzi activity of a concentrated ethanolic extract of Physalis angulata (EEPA). METHODS: Cytotoxicity to mammalian cells was determined using mouse peritoneal macrophages. The antiparasitic activity was evaluated against axenic epimastigote and bloodstream trypomastigote forms of T. cruzi, and against amastigote forms using T. cruzi-infected macrophages. Cell death mechanism was determined in trypomastigotes by flow cytometry analysis after annexin V and propidium iodide staining. The efficacy of EEPA was examined in vivo in an acute model of infection by monitoring blood parasitaemia and survival rate 30 days after treatment. The effect against trypomastigotes of EEPA and benznidazole acting in combination was evaluated. RESULTS: EEPA effectively inhibits the epimastigote growth (IC50 2.9 ± 0.1 µM) and reduces bloodstream trypomastigote viability (EC50 1.7 ± 0.5 µM). It causes parasite cell death by necrosis. EEPA impairs parasite infectivity as well as amastigote development in concentrations noncytotoxic to mammalian cells. In mice acutely-infected with T. cruzi, EEPA reduced the blood parasitaemia in 72.7%. When combined with benznidazole, EEPA showed a synergistic anti-T. cruzi activity, displaying CI values of 0.8 ± 0.07 at EC50 and 0.83 ± 0.1 at EC90. CONCLUSION: EEPA has antiparasitic activity against T. cruzi, causing cell death by necrosis and showing synergistic activity with benznidazole. These findings were reinforced by the observed efficacy of EEPA in reducing parasite load in T. cruzi-mice. Therefore, this represents an important source of antiparasitic natural products.

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RESUMO A espécie Physalis angulata L., pertencente à família Solanaceae, tem despontado como uma planta extremamente promissora para uso medicinal, em razão da produção do composto fisalina. No entanto, aspectos importantes do seu cultivo ainda permanecem pouco conhecidos. Assim, objetivou-se avaliar a influencia do fósforo para o seu crescimento. O experimento foi conduzido em Latossolo Amarelo de textura média, submetido a três doses de P: 8, 16 e 64 mg kg-1 de P. Usou-se um delineamento experimental inteiramente casualizado. Foram realizadas duas colheitas: aos 30 e 42 dias após o plantio. Verificou-se que as plantas cultivadas sob deficiência (8 mg kg-1 de P no solo) apresentaram 26,3 folhas, enquanto as cultivadas com a maior dose (64 mg kg-1 de P no solo) produziram 80,17 folhas. Aumento semelhante (201%) foi obtido para a área foliar, cujos valores variaram de 436,4 cm2a 1.313,9 cm2. Sob deficiência, a produção da massa seca total foi de apenas 1,72g, enquanto para a dose de 64 mg kg-1 de P no solo esse valor foi de 7,58g, significando um aumento de 340%. Plantas cultivadas com 8 mg kg-1 de P no solo não apresentaram flores ou frutos; já para o tratamento em que se utilizou 64 mg kg-1 de P no solo, o início do florescimento antecedeu ao das plantas cultivadas com 16 mg kg-1 de P no solo. A razão de área foliar e a área foliar específica foram maiores para as plantas cultivadas sob deficiência, enquanto a taxa de crescimento relativo e a taxa assimilatória líquida não foram estatisticamente influenciadas. Conclui-se que a deficiência de P no solo pode comprometer sobremaneira o crescimento das plantas de Physalis. Além disso, como a deficiência de P reduziu sensivelmente a produção de folhas, órgão responsável pela produção da fisalina, também é possível concluir que a deficiência de P pode, mesmo que indiretamente, reduzir a concentração desse importante composto na planta.

ABSTRACT The specie Physalis angulata L., who belongs to the Solanaceae family, has stand out as an extremely promising plant in terms of medical use, due to the production of the physalin compound. However, important aspects of its production still remain unknown. Thus the objective of this work was to assess the influence of phosphorus for the growth of these species. The experiment was performed with three doses of P (8, 16 e 64 mg kg-1 de P), in a completely randomized experimental design. Two harvests were performed: at 30 and 42 days after planting. The deficiency of P reduced the number of leaves and leaf area. The total dry matter (DM), as well as a MS from all parts of the plant, was significantly higher for plants grown under the highest levels of phosphorus. Plants grown with 8 mg P kg-1 showed no flowers or fruit; but, for the treatment which used 64 mg of P kg-1 the beginning of flowering happened before than with the plants grown with 16mg. The ratio of the leaf area and the specific leaf area were higher for plants grown under stress, while the values of the relative growth rate and the net assimilatory rate were not statistically influenced. We conclude that the deficiency of P in soil may considerably affect the growth of Physalis plants. Moreover, since the deficiency of P significantly reduced the leaf production, which is responsible for creating the physalin, it is also possible to conclude that P deficiency may, even indirectly, reduce the concentration of this important compound in the plant.

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UNLABELLED: Antileishmanial in vitro tests, as well as Ames and micronucleus assays were performed with a concentrated ethanolic extract of Physalis angulata (EEPA) RESULTS: EEPA did not present mutagenic effect in Salmonella typhimurium strains at concentration reaching 3000 µg/plate and did not induce mutagenic effects after two oral administrations with a 24h interval at a dose level of 2000 mg/kg. EEPA presented antileishmanial activity and presented an IC50 value of 5.35 ± 2.50 µg/mL and 4.50 ± 1.17 µg/mL against Leishmania amazonensis and Leishmania braziliensis promastigotes, respectively. In the cytotoxicity test against macrophages, the EEPA had a LC50 of 6.14 ± 0.59 µg/mL. Importantly, the IC50 against L. amazonensis intracellular amastigotes was 1.23 ± 0.11 µg/mL. CONCLUSION: EEPA extract is non-mutagenic and presented a promising pharmacological effect against Leishmania parasites.

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Physalis angulata is a specie of the Solanaceae family, which edible fruit is used in several countries of tropical and subtropical regions of the world as medicinal and fruit-tree. This review shows research over the last 30 years, about traditional uses, chemical constituents and pharmacology of this specie. The studies related to traditional uses show that P. angulata is known for its antimalarial, anti-inflammatory and post-partum treating properties. It presents the different pharmacological experiments in vitro and in vivo models that have been made, also the identification of phytochemical constituents with medicinal importance, the main being physalins and withanolides. Pharmacological studies have shown antiparasitic, anti-inflammatory, antimicrobial, antinociceptive, antimalarial, antileishmanial, immunosuppressive, antiasthmatic diuretic, and antitumor activities, thus validating its traditional uses and demonstrating the great potential of this specie for further development within the pharmaceutical industry.

Physalis angulata, es una especie de la familia Solanaceae, de frutos comestibles, que en diferentes países de regiones tropicales y subtropicales del mundo utilizan como medicinal y frutal. La presente revisión muestra las investigaciones realizadas durante los últimos 30 años, sobre los usos tradicionales, componentes químicos y farmacología de esta especie. Los estudios referidos a los usos tradicionales, muestran que la especie es conocida por propiedades antimaláricas, antiinflamatorias y en el tratamiento de postparto. Se muestran los diferentes experimentos farmacológicos de ensayos in vitro y modelos in vivo que se han realizado, asimismo la identificación de sus constituyentes fitoquímicos con importancia medicinal, siendo los principales las fisalinas y los witanólidos. Los estudios farmacológicos revelan que tiene actividad antiparasitaria, antiinflamatoria, antimicrobiana, antinociceptiva, antimalárica, antileishmania, inmunosupresor, antiasmático, diurético y antitumoral, validando de esta manera sus usos tradicionales y demostrando el gran potencial que tiene esta especie para un mayor desarrollo dentro de la industria farmacéutica.

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Extratos e frações de frutos e raízes de Physalis angulata L. (Solanaceae) foram ensaiados para encontrar atividade antimicrobiana. Aplicando o método de difusão em agar, todas as amostras foram testadas contra Staphylococcus aureus ATCC 6538. O extrato etanólico dos frutos apresentou atividade antibacteriana, a qual teve a atividade fototóxica estimada em cobaias quando expostas a luz ultravioleta, e não foram observados eritemas. Esses dados impulsionaram a pesquisar diferentes formas de obtenção de extratos da planta, com o objetivo de preparar formulações com atividade anti-séptica, que possam se apresentar mais eficazes e seguras, quando aplicadas no tratamento de doenças infecciosas.

Extracts and fractions of Physalis angulata L. prepared from fruits and roots were assayed to find out antimicrobial activity. Using the agar diffusion method all samples were tested against Staphylococcus aureus ATCC 6538. The ethanolic extract of the fruits displayed bacterial activity. Phototoxic property was estimated with guinea pigs when they were exposed to ultraviolet light, no erythemas were observed. These data encouraged us to look for different forms of extracts wich could be applied as a safe and effective antiseptic product.

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Neste trabalho realizou-se uma caracterização morfoanatômica comparativa, entre as folhas de Nicandra physalodes (L.) Gaertn. e Physalis angulata L. (Solanaceae), espécies de uso na medicina popular, cujas folhas e raízes são indicadas como diurético e antitérmico. As identificações botânicas e as morfodiagnoses macroscópicas foram realizadas após estudos morfológicos. Para as morfodiagnoses microscópicas realizaram-se cortes paradérmicos e transversais das folhas (lâmina e pecíolo), clarificados e posteriormente corados com safranina e safrablue. Além dos caracteres morfológicos da flor e do fruto, as duas espécies diferem pelos seguintes caracteres: vascularização da nervura principal em forma de arco em N. physalodes, e semicircular em P. angulata; drusas no mesofilo de P. angulata; tricomas glandular-estipitados com a glândula apical bisseriada em N. physalodes. As duas espécies distinguem-se principalmente pela morfologia da flor e fruto e pela anatomia do mesofilo e vascularização da nervura principal.

In this work a morphoanatomical comparative characterization was carried out between the leaves of Nicandra physalodes (L.) Gaertn. and Physalis angulata L. (Solanaceae) species used in folk medicine, whose leaves are indicated as diuretic and febrifuge. The botanical identification and macroscopical morphodiagnosis were made after morphological studies. The microscopical morphodiagnosis were carried out by paradermic and transversal cuts of the leaves (blade and petiole), clarified and coloured with safranin and astrablue. Moreover the morphological characters of the flower and fruit, of the two species can be distinguished by the following characters: N. physalodes has the bundle vascular of the middle vein in arc format; P. angulata has the vascular bundle of the middle vein in a semi-circle format; druses in the mesophyll of P. angulata; andthe presence of trichomes glandular-estipitate with bisseriate head in N. physalodes. The morphology of flower and fruit and the anatomy of mesophyll, the vascular bundles of middle vein are the principal distinctive characters between them.