RESUMO

Reducing phosphate fertilizer inputs while increasing food nutritional quality has been posited as a major challenge to decrease human undernourishment and ensure food security. In this context, quinoa has emerged as a promising crop due to its ability to tolerate different stress conditions and grow in marginal soils with low nutrient content, in addition to the exceptional nutritional quality of its grains. However, there is scarce information about the phosphorus acquisition capacity of quinoa roots. This work aimed to provide new insights into P acquisition and functional root traits, such as root biomass, rhizosphere pH, carboxylate exudation, and acid phosphatase activity of thirty quinoa genotypes grown under P limiting conditions (7 mg P kg-1). Significant genotypic variation was observed among genotypes, with average P accumulation ranging from 1.2 to 11.8 mg. The shoot biomass production varied more than 14 times among genotypes and was correlated with the P accumulation on shoots (r = 0.91). Despite showing high variability in root traits, only root biomass production highly correlated with P acquisition (r = 0.77), suggesting that root growth/morphology rather than the measured biochemical activity possesses a critical role in the P nutrition of quinoa.

RESUMO

The interaction of the alternative oxidase (AOX) pathway with nutrient metabolism is important for understanding how respiration modulates ATP synthesis and carbon economy in plants under nutrient deficiency. Although AOX activity reduces the energy yield of respiration, this enzymatic activity is upregulated under stress conditions to maintain the functioning of primary metabolism. The in vivo metabolic regulation of AOX activity by phosphorus (P) and nitrogen (N) and during plant symbioses with Arbuscular mycorrhizal fungi (AMF) and Rhizobium bacteria is still not fully understood. We highlight several findings and open questions concerning the in vivo regulation of AOX activity and its impact on plant metabolism during P deficiency and symbiosis with AMF. We also highlight the need for the identification of which metabolic regulatory factors of AOX activity are related to N availability and nitrogen-fixing legume-rhizobia symbiosis in order to improve our understanding of N assimilation and biological nitrogen fixation.

Assuntos

RESUMO

Objetive: Arsenic (As) and fluoride (F) are found in groundwater and soils around the world, causing different problems to crops. Because these elements compete against phosphorus (P) in soils and plants, their relationship is complex. The aim of this work was to study the oxidative stress of soybean plants subjected to different concentrations of As and F, and the effect of P. METHODS: The following 10 treatments were carried out in each of two soils with different P content: three As levels (low 10 mg As kg-1, medium 50 mg As kg-1 and high 100 mg As kg-1), three F levels (low 160 mg F kg-1, medium 250 mg F kg-1 and high 500 mg F kg-1) and three As + F levels (same concentrations), and the control treatment (soil with the background As and F concentrations) Lipid peroxidation, chlorophyll, gluthatione contents and antioxidant enzymes activities were determination. RESULTS: Increased lipid peroxidation and alterations in glutathione content, catalase, superoxide dismutase and peroxidase activities as well as in chlorophyll content revealed that As causes higher oxidative stress in plants grown in soils with low P content. CONCLUSION: Stress parameters in F treatments were less affected. Plants grown in soils enriched with P revealed a decrease in the toxic effects caused by As and F.

Assuntos

RESUMO

In Brazil, the campos rupestres occur over the Brazilian shield, and are characterized by acidic nutrient-impoverished soils, which are particularly low in phosphorus (P). Despite recognition of the campos rupestres as a global biodiversity hotspot, little is known about the diversity of P-acquisition strategies and other aspects of plant mineral nutrition in this region. To explore nutrient-acquisition strategies and assess aspects of plant P nutrition, we measured leaf P and nitrogen (N) concentrations, characterized root morphology and determined the percentage arbuscular mycorrhizal (AM) colonization of 50 dominant species in six communities, representing a gradient of soil P availability. Leaf manganese (Mn) concentration was measured as a proxy for carboxylate-releasing strategies. Communities on the most P-impoverished soils had the highest proportion of nonmycorrhizal (NM) species, the lowest percentage of mycorrhizal colonization, and the greatest diversity of root specializations. The large spectrum of leaf P concentration and variation in root morphologies show high functional diversity for nutritional strategies. Higher leaf Mn concentrations were observed in NM compared with AM species, indicating that carboxylate-releasing P-mobilizing strategies are likely to be present in NM species. The soils of the campos rupestres are similar to the most P-impoverished soils in the world. The prevalence of NM strategies indicates a strong global functional convergence in plant mineral nutrition strategies among severely P-impoverished ecosystems.

Assuntos

RESUMO

Common bean (Phaseolus vulgaris L.) is frequently grown in weathered soils with low phosphorus (P) availability, and this is one of the main limitations on its production. This study aimed to assess 20 common bean genotypes in a hydroponic system to select the best P concentration for inducing nutritional deficiency and to classify the genotypes in terms of nutrient utilization efficiency. The concentrations of P applied were 8.00, 4.00, 2.00 and 0.05 mg L¹. At 21 days, in the plot subjected to an application of the most severe stress, the 0.05 mg L¹ dose of P, had smaller plant size and early leaf abscission was observed. The 4.00 mg L¹ dose of P was the most efficient in inducing stress for discrimination of cultivars in terms of efficiency of use of P. The following genotypes: IAPAR 81, Carioca Comum, IAC Carioca Tybatã, IAC Imperador and G 2333 stood out as being efficient and responsive to P, while the two cultivars DOR 364 and Jalo Precoce were the most inefficient and unresponsive.(AU)

Assuntos

RESUMO

Common bean (Phaseolus vulgaris L.) is frequently grown in weathered soils with low phosphorus (P) availability, and this is one of the main limitations on its production. This study aimed to assess 20 common bean genotypes in a hydroponic system to select the best P concentration for inducing nutritional deficiency and to classify the genotypes in terms of nutrient utilization efficiency. The concentrations of P applied were 8.00, 4.00, 2.00 and 0.05 mg L¹. At 21 days, in the plot subjected to an application of the most severe stress, the 0.05 mg L¹ dose of P, had smaller plant size and early leaf abscission was observed. The 4.00 mg L¹ dose of P was the most efficient in inducing stress for discrimination of cultivars in terms of efficiency of use of P. The following genotypes: IAPAR 81, Carioca Comum, IAC Carioca Tybatã, IAC Imperador and G 2333 stood out as being efficient and responsive to P, while the two cultivars DOR 364 and Jalo Precoce were the most inefficient and unresponsive.

Assuntos

RESUMO

O pinhão manso tem atraído bastante interesse nos últimos anos por apresentar elevado potencial de fornecer óleo para produção de biocombustível e isso tem provocado a expansão rápida das áreas de cultivo em todo mundo. Este trabalho teve como objetivo avaliar a influência da irrigação com água de diferentes condutividades elétricas (CEa) e doses de fósforo sobre os componentes de produção de pinhão manso durante o terceiro ano de produção. Usou-se o delineamento em blocos casualizados em um fatorial (5x2) com quatro repetições, sendo cinco níveis de salinidade (CEa - 0,6 testemunha; 1,2; 1,8; 2,4 e 3,0dS m-1) da água de irrigação e duas doses de P2O5 (135 e 200g planta 1 ano-1), em que a água de 0,6dS m-1 refere-se à água de abastecimento e à dose de 135g planta-1, a recomendação para o pinhão manso. As plantas foram cultivadas em vasos com capacidade de 200L e irrigados a cada três dias. O número de dias para inflorescência, número de cachos planta-1, produtividade de sementes e o teor de óleo das sementes de pinhão manso foram afetados negativamente pelo o aumento da salinidade da água de irrigação. A cultura do pinhão manso irrigado com água com condutividade elétrica de 1,3dS m-1 reduz 10% da produtividade das sementes e consequentemente do teor de óleo em suas sementes. Somente o número de dias para inflorescência foi afetado pelas doses de fósforo. Verificou-se correlação positiva e significativa entre o peso de 100 sementes e o teor de óleo das sementes que, respectivamente, foram 90,26g e 36,39% no tratamento irrigado com a água de menor condutividade elétrica.

The physic nut has attracted considerable interest in recent years because of the high potential to provide oil for biofuel production and this has caused the rapid expansion of cultivated area worldwide. This research aimed to evaluate the influence of irrigation with water of different electrical conductivity (ECw) and two doses of phosphorus on the production components of physic nut during the third year of production. A randomized block design in a factorial (5x2) was adopted with four repetitions, with five levels of salinity (ECw - 0.6 control; 1.2; 1.8; 2.4 and 3.0dS m-1) of the irrigation water and two doses of P2O5 (135 and 200g plant-1 year-1), in which the water of 0.6dS m-1 refers to municipal supply water and the dose of 135g plant-1 is the recommended dose for physic nut. Plants were cultivated in recipient of 200L and irrigated at intervals of three days. The number of days for inflorescence, number of clusters plant-1, grain yield and oil content of physic nut seeds were negatively affected by the increasing salinity of the irrigated water. The cultivation of physic nut irrigated with electrical conductivity of 1.3dS m-1 reduces the productivity by 10% and consequently the seed oil content. Only the number of days for inflorescence was affected by phosphorus doses. A positive and significant correlation between weight of 100 seeds and oil content of seeds was observed and in the treatment with the lowest ECw the values obtained were respectively 90.26g and 36.39%.

RESUMO

The physic nut has attracted considerable interest in recent years because of the high potential to provide oil for biofuel production and this has caused the rapid expansion of cultivated area worldwide. This research aimed to evaluate the influence of irrigation with water of different electrical conductivity (ECw) and two doses of phosphorus on the production components of physic nut during the third year of production. A randomized block design in a factorial (5x2) was adopted with four repetitions, with five levels of salinity (ECw - 0.6 control; 1.2; 1.8; 2.4 and 3.0dS m-1) of the irrigation water and two doses of P2O5 (135 and 200g plant-1 year-1), in which the water of 0.6dS m-1 refers to municipal supply water and the dose of 135g plant-1 is the recommended dose for physic nut. Plants were cultivated in recipient of 200L and irrigated at intervals of three days. The number of days for inflorescence, number of clusters plant-1, grain yield and oil content of physic nut seeds were negatively affected by the increasing salinity of the irrigated water. The cultivation of physic nut irrigated with electrical conductivity of 1.3dS m-1 reduces the productivity by 10% and consequently the seed oil content. Only the number of days for inflorescence was affected by phosphorus doses. A positive and significant correlation between weight of 100 seeds and oil content of seeds was observed and in the treatment with the lowest ECw the values obtained were respectively 90.26g and 36.39%.

O pinhão manso tem atraído bastante interesse nos últimos anos por apresentar elevado potencial de fornecer óleo para produção de biocombustível e isso tem provocado a expansão rápida das áreas de cultivo em todo mundo. Este trabalho teve como objetivo avaliar a influência da irrigação com água de diferentes condutividades elétricas (CEa) e doses de fósforo sobre os componentes de produção de pinhão manso durante o terceiro ano de produção. Usou-se o delineamento em blocos casualizados em um fatorial (5x2) com quatro repetições, sendo cinco níveis de salinidade (CEa - 0,6 testemunha; 1,2; 1,8; 2,4 e 3,0dS m-1) da água de irrigação e duas doses de P2O5 (135 e 200g planta 1 ano-1), em que a água de 0,6dS m-1 refere-se à água de abastecimento e à dose de 135g planta-1, a recomendação para o pinhão manso. As plantas foram cultivadas em vasos com capacidade de 200L e irrigados a cada três dias. O número de dias para inflorescência, número de cachos planta-1, produtividade de sementes e o teor de óleo das sementes de pinhão manso foram afetados negativamente pelo o aumento da salinidade da água de irrigação. A cultura do pinhão manso irrigado com água com condutividade elétrica de 1,3dS m-1 reduz 10% da produtividade das sementes e consequentemente do teor de óleo em suas sementes. Somente o número de dias para inflorescência foi afetado pelas doses de fósforo. Verificou-se correlação positiva e significativa entre o peso de 100 sementes e o teor de óleo das sementes que, respectivamente, foram 90,26g e 36,39% no tratamento irrigado com a água de menor condutividade elétrica.

RESUMO

The physic nut has attracted considerable interest in recent years because of the high potential to provide oil for biofuel production and this has caused the rapid expansion of cultivated area worldwide. This research aimed to evaluate the influence of irrigation with water of different electrical conductivity (ECw) and two doses of phosphorus on the production components of physic nut during the third year of production. A randomized block design in a factorial (5x2) was adopted with four repetitions, with five levels of salinity (ECw - 0.6 control; 1.2; 1.8; 2.4 and 3.0dS m-1) of the irrigation water and two doses of P2O5 (135 and 200g plant-1 year-1), in which the water of 0.6dS m-1 refers to municipal supply water and the dose of 135g plant-1 is the recommended dose for physic nut. Plants were cultivated in recipient of 200L and irrigated at intervals of three days. The number of days for inflorescence, number of clusters plant-1, grain yield and oil content of physic nut seeds were negatively affected by the increasing salinity of the irrigated water. The cultivation of physic nut irrigated with electrical conductivity of 1.3dS m-1 reduces the productivity by 10% and consequently the seed oil content. Only the number of days for inflorescence was affected by phosphorus doses. A positive and significant correlation between weight of 100 seeds and oil content of seeds was observed and in the treatment with the lowest ECw the values obtained were respectively 90.26g and 36.39%.

O pinhão manso tem atraído bastante interesse nos últimos anos por apresentar elevado potencial de fornecer óleo para produção de biocombustível e isso tem provocado a expansão rápida das áreas de cultivo em todo mundo. Este trabalho teve como objetivo avaliar a influência da irrigação com água de diferentes condutividades elétricas (CEa) e doses de fósforo sobre os componentes de produção de pinhão manso durante o terceiro ano de produção. Usou-se o delineamento em blocos casualizados em um fatorial (5x2) com quatro repetições, sendo cinco níveis de salinidade (CEa - 0,6 testemunha; 1,2; 1,8; 2,4 e 3,0dS m-1) da água de irrigação e duas doses de P2O5 (135 e 200g planta 1 ano-1), em que a água de 0,6dS m-1 refere-se à água de abastecimento e à dose de 135g planta-1, a recomendação para o pinhão manso. As plantas foram cultivadas em vasos com capacidade de 200L e irrigados a cada três dias. O número de dias para inflorescência, número de cachos planta-1, produtividade de sementes e o teor de óleo das sementes de pinhão manso foram afetados negativamente pelo o aumento da salinidade da água de irrigação. A cultura do pinhão manso irrigado com água com condutividade elétrica de 1,3dS m-1 reduz 10% da produtividade das sementes e consequentemente do teor de óleo em suas sementes. Somente o número de dias para inflorescência foi afetado pelas doses de fósforo. Verificou-se correlação positiva e significativa entre o peso de 100 sementes e o teor de óleo das sementes que, respectivamente, foram 90,26g e 36,39% no tratamento irrigado com a água de menor condutividade elétrica.

RESUMO

Composition and the role of root flavonoids in the regulation of mycorrhizal symbiosis are still poorly understood. Several flavonoids stimulate spore germination, mycelia growth and root colonization by arbuscular mycorrhizal fungi (AMF), and both root colonization and flavonoid composition are affected by plant nutritional status. Effects of AMF on the occurrence and content of aromatic secondary metabolites in the roots of passion fruit seedlings grown under two levels of phosphorus (P) fertilization (10 and 50 mg kg-1 of phosphorus) was studied. Seedlings were inoculated with Glomus clarum and a population of native fungi from a passion fruit plantation. Methanolic extracts of passion fruit seedlings roots were analyzed by high performance liquid chromatography (HPLC). It was recorded the occurrence of several compounds, possibly flavonoids, with seven major peaks. The root contents of the compound with a retention time of 4.5 minutes, varied in response to the root colonization by different mycorrhizal fungi, and the contents of two compounds with retention times of 3.4 and 18.9 minutes varied due to the poor plant growth and nutritional status. Passion fruit seedlings have several aromatic compounds, and their contents were correlated with root colonization by different mycorrhizal fungi, the reduced seedling growth due to nutritional stress, and/or the plant defense responses to the fungi.

Os flavonóides nas raízes e seu papel na regulação da simbiose com fungos micorrízicos não são bem conhecidos. Vários flavonóides estimulam a germinação de esporos, crescimento micelial e colonização micorrízica. Ambos, a colonização micorrízica e a composição de flavonóides nas raízes são afetados pelo estado nutricional da planta. Avaliou-se o efeito de fungos micorrízicos arbusculares sobre a ocorrência e concentração de substâncias, possivelmente metabólitos aromáticos secundários pertencentes à classe dos flavonóides, em raízes de mudas de maracujazeiro amarelo, crescidas sob dois níveis de fósforo (10 e 50 mg kg-1). As mudas foram inoculadas com Glomus clarum e uma população nativa de fungos micorrízicos de um plantio de maracujá do município de São João da Barra, RJ. Extratos de raízes em metanol foram analisados por cromatografia líquida de alta eficiência. Os cromatogramas mostraram a ocorrência de diversos compostos, possivelmente da classe dos flavonóides, diferenciados no cromatograma em sete picos principais. Foi observado que os conteúdos nas raízes do composto com tempo de retenção de 4,5 minutos, variou em função da colonização micorrízica pelas diferentes espécies de fungos e o conteúdo dos dois compostos com tempo de retenção de 3,4 e 18,9 minutos, variou com o baixo crescimento da planta e o estresse nutricional. Mudas de maracujazeiro apresentam diversos compostos aromáticos, possivelmente da classe dos flavonóides, e o seu conteúdo varia com a colonização radicular por diferentes espécies de fungos micorrízicos, o baixo crescimento da planta devido a estresse nutricional de fósforo e/ou a resposta de defesa da planta ao fungo.

RESUMO

Composition and the role of root flavonoids in the regulation of mycorrhizal symbiosis are still poorly understood. Several flavonoids stimulate spore germination, mycelia growth and root colonization by arbuscular mycorrhizal fungi (AMF), and both root colonization and flavonoid composition are affected by plant nutritional status. Effects of AMF on the occurrence and content of aromatic secondary metabolites in the roots of passion fruit seedlings grown under two levels of phosphorus (P) fertilization (10 and 50 mg kg-1 of phosphorus) was studied. Seedlings were inoculated with Glomus clarum and a population of native fungi from a passion fruit plantation. Methanolic extracts of passion fruit seedlings roots were analyzed by high performance liquid chromatography (HPLC). It was recorded the occurrence of several compounds, possibly flavonoids, with seven major peaks. The root contents of the compound with a retention time of 4.5 minutes, varied in response to the root colonization by different mycorrhizal fungi, and the contents of two compounds with retention times of 3.4 and 18.9 minutes varied due to the poor plant growth and nutritional status. Passion fruit seedlings have several aromatic compounds, and their contents were correlated with root colonization by different mycorrhizal fungi, the reduced seedling growth due to nutritional stress, and/or the plant defense responses to the fungi.

Os flavonóides nas raízes e seu papel na regulação da simbiose com fungos micorrízicos não são bem conhecidos. Vários flavonóides estimulam a germinação de esporos, crescimento micelial e colonização micorrízica. Ambos, a colonização micorrízica e a composição de flavonóides nas raízes são afetados pelo estado nutricional da planta. Avaliou-se o efeito de fungos micorrízicos arbusculares sobre a ocorrência e concentração de substâncias, possivelmente metabólitos aromáticos secundários pertencentes à classe dos flavonóides, em raízes de mudas de maracujazeiro amarelo, crescidas sob dois níveis de fósforo (10 e 50 mg kg-1). As mudas foram inoculadas com Glomus clarum e uma população nativa de fungos micorrízicos de um plantio de maracujá do município de São João da Barra, RJ. Extratos de raízes em metanol foram analisados por cromatografia líquida de alta eficiência. Os cromatogramas mostraram a ocorrência de diversos compostos, possivelmente da classe dos flavonóides, diferenciados no cromatograma em sete picos principais. Foi observado que os conteúdos nas raízes do composto com tempo de retenção de 4,5 minutos, variou em função da colonização micorrízica pelas diferentes espécies de fungos e o conteúdo dos dois compostos com tempo de retenção de 3,4 e 18,9 minutos, variou com o baixo crescimento da planta e o estresse nutricional. Mudas de maracujazeiro apresentam diversos compostos aromáticos, possivelmente da classe dos flavonóides, e o seu conteúdo varia com a colonização radicular por diferentes espécies de fungos micorrízicos, o baixo crescimento da planta devido a estresse nutricional de fósforo e/ou a resposta de defesa da planta ao fungo.

RESUMO

To evaluate the effect of phosphorus supplementation on 32P uptake by red blood cells in vitro as diagnosis of subclinical P deficiency, twelve calves averaging 191 kg were separated from the herd for 12 weeks. During the first 6 weeks, the animals were maintained on a pasture without phosphorus supplementation. During the next 6-week period, the animals were supplemented with a commercial mineral salt with 6.0% total P. Four ml of freshly drawn whole blood were obtained at 2-week intervals and incubated with 1.85 MBq 32P. After incubation at 38°C for a 3-hour period, the cells were separated from the plasma and washed three times with an isotonic saline solution and their radioactivity was determined through the Cerenkov effect. Effects of treatment upon the levels of P blood plasma, blood glucose and P excretion were also investigated and these parameters increased significantly with P supplementation. The average rates of 32P uptake by the erythrocytes were 7.04 ± 1.20 and 5.91 ± 1.24% (P 0,05), for treatments without and with P supplementation, respectively. The method showed to be suitable as an additional parameter to estimate the P status in ruminants.

Com o objetivo de avaliar o efeito da suplementação de P sobre a incorporação de 32P pelos eritrócitos de bovinos como método para diagnóstico da deficiência subclínica desse mineral, doze bezerros mestiços pesando em média 191 kg foram separados do rebanho por 12 semanas. Durante as 6 primeiras semanas os animais consumiram apenas a pastagem, sem receber suplementação mineral. Após esse período, os animais foram suplementados com uma mistura mineral comercial contendo 6,0% P por mais 6 semanas, permanecendo na mesma pastagem. Amostras de sangue foram coletadas a cada 2 semanas. Quatro ml de sangue total foram incubados a 38°C com 1,85 MBq de 32P por 3 horas. As células foram separadas do plasma por centrifugação e lavadas com solução salina por 3 vezes. A radioatividade nas células foi detectada através do efeito Cerenkov. Os efeitos dos tratamentos foram também investigados sobre os níveis de P e glucose no plasma e P nas fezes. Houve aumento significativo nesses parâmetros à medida em que os animais foram suplementados. As taxas de incorporação de 32P pelos eritrócitos tiveram valores médios de 7,04 ± 1,20 e 5,91 ± 1,24% (P 0,05), para as fases sem e com suplementação de P, respectivamente. O método mostrou ser promissor como um parâmetro adicional na avaliação do estado nutricional de P em ruminantes.

RESUMO

To evaluate the effect of phosphorus supplementation on 32P uptake by red blood cells in vitro as diagnosis of subclinical P deficiency, twelve calves averaging 191 kg were separated from the herd for 12 weeks. During the first 6 weeks, the animals were maintained on a pasture without phosphorus supplementation. During the next 6-week period, the animals were supplemented with a commercial mineral salt with 6.0% total P. Four ml of freshly drawn whole blood were obtained at 2-week intervals and incubated with 1.85 MBq 32P. After incubation at 38°C for a 3-hour period, the cells were separated from the plasma and washed three times with an isotonic saline solution and their radioactivity was determined through the Cerenkov effect. Effects of treatment upon the levels of P blood plasma, blood glucose and P excretion were also investigated and these parameters increased significantly with P supplementation. The average rates of 32P uptake by the erythrocytes were 7.04 ± 1.20 and 5.91 ± 1.24% (P 0,05), for treatments without and with P supplementation, respectively. The method showed to be suitable as an additional parameter to estimate the P status in ruminants.

Com o objetivo de avaliar o efeito da suplementação de P sobre a incorporação de 32P pelos eritrócitos de bovinos como método para diagnóstico da deficiência subclínica desse mineral, doze bezerros mestiços pesando em média 191 kg foram separados do rebanho por 12 semanas. Durante as 6 primeiras semanas os animais consumiram apenas a pastagem, sem receber suplementação mineral. Após esse período, os animais foram suplementados com uma mistura mineral comercial contendo 6,0% P por mais 6 semanas, permanecendo na mesma pastagem. Amostras de sangue foram coletadas a cada 2 semanas. Quatro ml de sangue total foram incubados a 38°C com 1,85 MBq de 32P por 3 horas. As células foram separadas do plasma por centrifugação e lavadas com solução salina por 3 vezes. A radioatividade nas células foi detectada através do efeito Cerenkov. Os efeitos dos tratamentos foram também investigados sobre os níveis de P e glucose no plasma e P nas fezes. Houve aumento significativo nesses parâmetros à medida em que os animais foram suplementados. As taxas de incorporação de 32P pelos eritrócitos tiveram valores médios de 7,04 ± 1,20 e 5,91 ± 1,24% (P 0,05), para as fases sem e com suplementação de P, respectivamente. O método mostrou ser promissor como um parâmetro adicional na avaliação do estado nutricional de P em ruminantes.