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OBJECTIVE: Patients with Richieri-Costa-Pereira syndrome (RCPS) present severe craniofacial alterations and frequently require orthodontic and surgical procedures. Thus, this study aims to describe the craniofacial relationships in patients with RCPS. DESIGN: Panoramic radiographs and lateral cephalometric teleradiographs of 7 patients with RCPS and 7 age- and sex-matched nonsyndromic patients were analyzed. Cephalometric measurements were used to determine the size of apical bases, the relationship between them, the pattern of craniofacial growth, and the facial heights of the patients. Interobservers' concordance was verified by intraclass coefficient. For comparison between the groups, paired t test was employed. P values <.05 indicated statistical significance. RESULTS: Average age of patients with RCPS was 18.5 years. Six patients were female. All patients with RCPS had Pierre-Robin sequence while 2 also presented cleft mandible. Most patients with RCPS had missing lower central incisors (100%), lower lateral incisors (85.7%), lower second premolars (85.7%), and/or upper lateral incisors (57.1%). Concordance between observers was excellent for all cephalometric measurements (0.87-0.99). Patients with RCPS presented severe craniofacial alterations when compared to control group: sella-nasion-B point (SNB) angle (73.8o ± 4.86o vs 78.85o ± 4.53o, P = .029), maxillary length (7.89 cm ± 0.58 cm vs 16.36 cm ± 0.75 cm, P = .001), mandibular length (9.90 cm ± 0.46 cm vs 20.61 cm ± 0.45 cm, P = .001), upper anterior face height (5.41 cm ± 0.50 cm vs 9.40 cm ± 0.47 cm, P = .001), lower anterior face height (5.48 cm ± 0.75 cm vs 11.66 cm ± 0.55 cm, P = .001), and posterior face height (6.70 cm ± 0.33 cm vs 13.65 cm ± 1.06 cm, P = .001). There was no difference in SNB, A point-nasion-B point, pogonion-nasion-B point, and mandibular place angles between the groups (P > .05). CONCLUSION: Patients with RCPS present deficient development of maxilla and mandible when compared with nonsyndromic patients.
Assuntos
Pé Torto Equinovaro , Deformidades Congênitas da Mão , Síndrome de Pierre Robin , Adolescente , Feminino , Humanos , MaxilaRESUMO
Resumo O objetivo deste artigo é revisitar um autor e uma autora na perspectiva de analisar em dois textos as narrativas construídas, No primeiro, da elaboração em medicina social das teorias sociológicas e, no segundo, da pesquisa qualitativa em saúde. Toma-se como objeto uma dimensão macro das narrativas, no sentido dado por Maines das possibilidades de construção de uma sociologia das narrativas e de uma narrativa da sociologia. Os textos analisados são da década de 1980, de autoria de JCM Pereira e MCS Minayo.
Abstract The purpose of this article is to revisit two authors with the intent to analyze the narratives constructed in two texts. In the first one, the narrative analyzed was the one pertaining to the elaboration of sociological theories in social medicine, and in the second, it was the one related to qualitative research in Health. The macro dimension of narratives, in the sense given by Maines of the possibilities of building a sociology of narratives and a narrative of sociology, will be the object of this study. The texts analyzed were written in the 1980s by JCM Pereira and MCS Minayo.
Assuntos
Humanos , Medicina Social , Narração , Teoria Social , Pesquisa QualitativaRESUMO
RESUMO: O presente artigo é resultado de uma pesquisa que teve como objetivo investigar, em uma perspectiva de educação comparada, a circularidade de um pensamento educacional e modelos de formação docente envolvendo o Curso Normal (1951-1957) e os Cursos de Especialização de Professores de Deficientes Auditivos (1957-1972/1981-1985), mantidos pelo Instituto Nacional de Surdos-Mudos (INSM)/Instituto Nacional de Educação de Surdos (INES) e os cursos congêneres oferecidos pelo Instituto Jacob Rodrigues Pereira - IJRP (1952-1956/1961-1963/1982-1984), em Portugal. A investigação foi de cunho qualitativo e as fontes consultadas foram: textos legislativos, anteprojetos, regulamentos, regimentos, grades curriculares, fichas de matrículas e históricos das ex-normalistas e ex-cursistas, fichas funcionais de professores, periódicos, livros, manuais, ordens de serviço, relatórios e anais de Campanha. Esses documentos foram encontrados no Acervo Histórico, nos Arquivos corrente e permanente do INES, no Brasil, e no Centro de Documentação, Acervo Histórico e Biblioteca do Centro Cultural da Casapiano e na Biblioteca Nacional de Portugal, em Lisboa. Também houve a produção de entrevistas semiestruturadas com doze docentes, entre ex-normalistas, ex-cursistas e ex-professores dos cursos investigados, à luz da metodologia da História Oral, por esta contribuir para a compreensão dos processos histórico-sociais. A pesquisa identificou elementos que evidenciaram a circulação de um pensamento educacional e modelos de formação docente, na área do ensino de surdos, que conectaram e entrelaçaram, de forma indireta e direta, as instituições pesquisadas, refletindo realidades globais que foram fruto de como os agentes escolares/institucionais se relacionaram, compartilhando conhecimentos, calcados na matriz oralista, que ultrapassaram as escalas nacionais.
ABSTRACT: The present paper is the result of a research that had the objective to investigate, in a comparative education perspective, educational mindsets and teacher qualification models involving the Escola Normal [Undergraduate Teacher-training School] (1951-1957) and the Cursos de Especialização de Professores de Deficientes Auditivos [Specialization Programs for Teachers of Students with Hearing Impairment] (1957-1972/1981-1985), offered by the Instituto Nacional dos Surdos-Mudos (INSM)/Instituto Nacional de Educação de Surdos (INES), as well as by their counterpart institutions -Instituto Jacob Rodrigues Pereira (IJRP) (1952-1956/1961-1963/1982-1984), in Portugal. This qualitative research has used as sources legislative texts, preliminary drafts, regulations, by-laws, syllabuses, student registration forms, school transcripts of ex-training-teachers, teacher employment and performance records, journals, books, manuals, work orders, reports and Campaign records found. These documents were found in the Historical Collection, INES current and permanent Archives, in Brazil, as well as in the Document Centre, Historical Collection and Library of the Cultural Centre of Casapiano and the National Library in Lisbon, Portugal. Semi-structured interviews were also conducted with twelve ex-training-teachers and teachers of the investigated institutions and programs, in the light of the Oral History methodology, for it is believed to provide qualitative interpretations of historical and social processes. The research identified elements that evidenced the interplay between their similar educational mindsets and teacher qualification models in the field of teaching students with hearing impairment, that have directly or indirectly connected and intermeshed the above mentioned institutions, and reflect global realities that resulted from the way through which institutional/school agents interacted and shared knowledge, embedded in the oral history matrix, that went beyond national borders.
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Richieri-Costa-Pereira syndrome is a rare autosomal recessive acrofacial dysostosis that has been mainly described in Brazilian individuals. The cardinal features include Robin sequence, cleft mandible, laryngeal anomalies and limb defects. A biallelic expansion of a complex repeated motif in the 5' untranslated region of EIF4A3 has been shown to cause this syndrome, commonly with 15 or 16 repeats. The only patient with mild clinical findings harbored a 14-repeat expansion in 1 allele and a point mutation in the other allele. This proband is described here in more details, as well as is his affected sister, and 5 new individuals with Richieri-Costa-Pereira syndrome, including a patient from England, of African ancestry. This study has expanded the phenotype in this syndrome by the observation of microcephaly, better characterization of skeletal abnormalities, less severe phenotype with only mild facial dysmorphisms and limb anomalies, as well as the absence of cleft mandible, which is a hallmark of the syndrome. Although the most frequent mutation in this study was the recurrent 16-repeat expansion in EIF4A3, there was an overrepresentation of the 14-repeat expansion, with mild phenotypic expression, thus suggesting that the number of these motifs could play a role in phenotypic delineation.
Assuntos
Pé Torto Equinovaro/genética , RNA Helicases DEAD-box/genética , Fator de Iniciação 4A em Eucariotos/genética , Deformidades Congênitas da Mão/genética , Laringe/fisiopatologia , Deformidades Congênitas dos Membros/genética , Síndrome de Pierre Robin/genética , Adolescente , Adulto , Alelos , Brasil/epidemiologia , Criança , Pé Torto Equinovaro/epidemiologia , Pé Torto Equinovaro/fisiopatologia , Expansão das Repetições de DNA/genética , Inglaterra/epidemiologia , Extremidades/fisiopatologia , Feminino , Genótipo , Deformidades Congênitas da Mão/epidemiologia , Deformidades Congênitas da Mão/fisiopatologia , Humanos , Laringe/anormalidades , Deformidades Congênitas dos Membros/fisiopatologia , Masculino , Fenótipo , Síndrome de Pierre Robin/epidemiologia , Síndrome de Pierre Robin/fisiopatologia , Mutação Puntual/genética , Adulto JovemRESUMO
Species in the genus Calonectria (Hypocreales) represent an important group of plant pathogenic fungi that cause serious losses to plant crops in tropical and subtropical climates. Calonectria leaf blight is currently one of the main impediments to Eucalyptus cultivation in Brazil, and various species of Calonectria have been associated with this disease. Since most previous identifications were solely based on morphological characters, much of the published literature needs to be re-evaluated. The aim of this study was thus to identify and determine the phylogenetic relationships among species that occur in the Eucalyptus growing regions of Brazil by using partial sequences of the ß-tubulin, calmodulin, translation elongation factor 1-α and histone H3 gene regions. Based on extensive collections from soil and infected eucalypt leaf samples from plantations, phylogenetic inference revealed the Ca. pteridis complex to be the most common species complex present in Eucalyptus plantations in Brazil. By elucidating taxa in the Ca. pteridis, Ca. cylindrospora and Ca. candelabra species complexes, 20 novel Calonectria species were identified, and a new name in Calonectria provided for Cylindrocladium macrosporum as Ca. pseudopteridis.
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Richieri-Costa and Pereira syndrome, described in 1992, comprises short stature, Robin sequence, cleft mandible, limb malformations, and short larynx, deformed or lack of epiglottis, and abnormal aryepiglottic folds. There are 32 reported cases, only one described outside Brazil. We describe a 4-month-old boy with the most severe phenotype yet reported.
Assuntos
Pé Torto Equinovaro/patologia , Deformidades Congênitas da Mão/patologia , Síndrome de Pierre Robin/patologia , Adolescente , Adulto , Brasil , Pé Torto Equinovaro/diagnóstico por imagem , Feminino , Deformidades Congênitas da Mão/diagnóstico por imagem , Humanos , Lactente , Masculino , Fenótipo , Síndrome de Pierre Robin/diagnóstico por imagem , Tomografia Computadorizada por Raios XRESUMO
Artículo que presenta una discusión que busca justificar la presencia de aves silvestres dentro de la ciudad de Pereira, enfáticamente las especies de aves rapaces, migratorias o bajo algún grado de amenaza, buscando ampliar la discusión de resultados presentada en el artículo predecesor llamado "Revisión a la diversidad ornitológica de Pereira". Se registraron catorce(14) especies de aves rapaces (es decir pertenecientes a las familias Cathartidae, Pandionidae, Accipitridae y Falconidae) las cuales deben su presencia a que no dependen de bosque y pueden sobrevivir en zonas urbanas; veintiséis (26) especies migratorias de las cuales, a excepción de tres especies consideradas raras, todas son comunes o bastantes comunes y la mayoría frecuenta áreas abiertas; siete (7)especies bajo algún grado de amenaza(amenaza que se debe principalmente a la destrucción de su hábitat) consideradas de por sí como raras y locales y cuatro (4) especies endémicas de las cuales solo una es considerada como rara, todas las demás son comunes y dentro de la ciudad encuentran los diversos tipos de hábitat requeridos por estas especies. Se discute y concluye que en general las especies abordadas son de áreas abiertas o no dependen de bosques, tolerantes a ecosistemas intervenidos y/o presentan cierta plasticidad de nicho con sus necesidades de hábitat y son capaces de encontrar sus requerimientos ecológicos dentro de las zonas urbanas. Así mismo que la heterogeneidad de hábitats y la estructura de vegetación variada de las zonas urbanas contribuyen a su riqueza de especies.
The article presents a discussion that seeks to justify the presence of wild birds in the city of Pereira -emphatically birds of prey, migratory birds, or birds under some degree of threat- seeking to broaden the discussion of results presented in the previous article "Revision to Ornithological Diversity in Pereira." Fourteen (14) species of birds of prey (this is to say belonging to the families Cathartidae, Pandionidae, Accipitridae and Falconidae) which owe their presence to the fact that they do not depend on forests and can survive in urban areas; twenty-six (26) migratory species which, except for three species considered rare, all are common or fairly common and most frequent in open areas; seven (7) species under some degree of threat (threat mainly due to habitat destruction) considered as rare and local; and four (4) endemic species of which only one is considered rare; all others are common and within the city they find the different types of habitat they require. It is discussed and concluded that in general the species dealt with belong to open areas and do not depend on forests, are tolerant to intervened ecosystems and/or have some niche plasticity with their habitat needs and are able to find their ecological requirements in urban areas. Also that the habitat heterogeneity and the varied vegetation structure in urban areas contribute to the richness of species.
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Humanos , Animais , Aves , Aves Predatórias , Ameaças , Locusta migratoriaRESUMO
Introducción: La infección VIH/SIDA continúa siendo un grave problema de salud pública mundial. Colombia es uno de los países considerablemente afectado en Latinoamérica, con municipios de alta incidencia, entre los cuales se encuentra Pereira. Materiales y métodos: Evaluación de los principales indicadores epidemiológicos (estimaciones de incidencia, mortalidad y letalidad) de la infección VIH/SIDA, período 1998-2011, Municipio Pereira, Risaralda, Colombia. Resultados: Durante el período se registraron 1471 casos (promedio 105,1±27,6/año, intervalo 51-159); tendencia al incremento (r2=0,3028; p=0,0415), especialmente entre 2006-2011 (r2=0,845; p=0,0095). La incidencia osciló entre 11,8 (2001) a 34,6 (2011) casos/100.000 hab (IC95% 29,0-40,0), sin tendencia al incremento entre 1998-2005 (r2=0,0351; p=0,6567), y aumentando significativamente de 2006-2011 (r2=0,8318; p=0,0113). Se registraron 631 muertes (promedio 45,1±8,9/año, intervalo 32-64), sin variación significativa entre 1998-2005 (r2=0,042; p=0,6262); de 2006-2011 se redujeron significativamente (r2=0,7016; p=0,0374). La mortalidad osciló entre 7,0 (2010) a 14,3 (2006) muertes/100.000 hab (IC95% 11,0-18,0), disminuyendo significativamente de 2006-2011 (r2=0,7251; p=0,0314). La letalidad en el período osciló entre 21,9% (2010) a 88,2% (2001) (IC95% 78,41-98,06). Discusión: A pesar que la incidencia del VIH/SIDA aun va en aumento, la letalidad y la mortalidad van disminuyendo progresivamente. Esto obedece a múltiples factores que incluyen mejor cobertura diagnóstica y terapéutica, mayor acceso al sistema de salud, más disponibilidad de información, manejo multidisciplinario apropiado y mejor capacitado. Sin embargo, en promoción y prevención aun quedan grandes retos por asumir, que incluyen más participación comunitaria en los procesos de acceso a la información, de vigilancia epidemiológica, de evaluación en la calidad de la prestación y satisfacción en el uso de servicios relacionados al programa de VIH/SIDA.
Introduction: HIV infection/AIDS continue to be a highly relevant global public health problem. Colombia is one country, in Latinamerica, with a considerable epidemiology, with high incidence municipalities; among those is included Pereira. Materials and methods: Assessment of main epidemiological indicators (estimations of incidence, mortality and lethality) of HIV infection/AIDS, period 1998-2011, Municipality Pereira, Risaralda, Colombia. Results: During the period a total of 1471 cases were recorded (mean 105.1±27.6/year, range 51-159); increasing (r2=0.3028; p=0.0415), specially between 2006-2011 (r2=0.845; p=0.0095). Incidence oscillated between 11.8 (2001) to 34.6 (2011) cases/100,000 pop (95%CI 29.0-40.0), without a significant increase between 1998-2005 (r2=0.0351; p=0.6567), and increasing significantly between 2006-2011 (r2=0.8318; p=0.0113). A total of 631 deaths were recorded (mean 45.1±8.9/year, range 32-64), no significant variation between 1998-2005 (r2=0.042; p=0.6262); from 2006-2011 were significantly reduced (r2=0.7016; p=0.0374). Mortality oscillated from 7.0 (2010) to 14.3 (2006) deaths/100,000 pop (95%CI 11.0-18.0), significantl decreasing between 2006-2011 (r2=0.7251; p=0.0314). Lethality (CFR) in the period oscillated from 21.9% (2010) to 88.2% (2001) (95%CI 8.41-98.06). Discussion: Although HIV/AIDS incidence is increasing, lethality and mortality are progressively decreasing. This could be related to multiple factors that include a better diagnostic and therapeutic coverage, higher access to the health care system, more information availability, as well appropriate and skilled multidisciplinary management. However, at promotion and prevention there are still great challenges to achieve, that include more communitary participation in the processes of information access, epidemiological surveillance, quality assessment at the use of health care services related to HIV/AIDS, among others.
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Humanos , HIV , Epidemiologia , Síndrome da Imunodeficiência Adquirida , Vigilância em Desastres , ColômbiaRESUMO
Objetivou-se, no presente trabalho, através de correlação de Pearson e análise de trilha, identificar variáveis para caracterizar porta-enxertos ananizantes para a cultura da pereira (Pyrus communis L.). Neste experimento foram utilizadas 49 plantas de pereira, plantadas nos canteiros do Departamento de Fitotecnia da FAEM/UFPel. As plantas foram avaliadas na época do seu crescimento vegetativo, segundo parâmetros descritos em instruções do Ministério da Agricultura, Pecuária e Abastecimento. Pela correlação de Pearson, as variáveis VP, NRP, HCP e FCNPRCL se destacaram. Na análise de trilha, a variável número de lenticelas obteve maior efeito positivo sobre VP, NRP e FCNPRCL, a variável ramificação do ramo demonstrou-se com efeito positivo sobre HCP, sendo essas duas variáveis consideradas eficazes no processo de seleção de porta-enxerto juntamente com as variáveis básicas.
The objective of this work was to identify, through the Pearson correlation and path analysis, variables to characterize rootstocks suitable for the cultivation of the pear (Pyrus communis L.). In this experiment 49 pear specimens were used, planted in flowerbeds at the Phytotechny Department. The plants were evaluated at the time of growth, according to parameters described in the instructions of the Ministério da Agricultura, Pecuária e Abastecimento. According to the Pearson correlation, the variables VP, NRP, ETA and FCNPRCL stood out. In path analysis, the variable number of lenticels had a more positive effect on VP, NRP and FCNPRCL, variable branch of the industry had a more positive effect on ETA. Both variables were considered effective in the process of selection of rootstock together with the basic variables.
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O artigo descreve a história da pereirina, um alcaloide extraído das cascas da Geissospermum vellosii Allemão, uma espécie de planta brasileira da família Apocynaceae, conhecida popularmente como Pau-pereira. A classificação botânica desta espécie e o emprego da pereirina na medicina popular foram as razões que acirraram a disputa entre os farmacêuticos brasileiros e estrangeiros pela glória de sua descoberta no Brasil do século XIX. Neste estudo constatou-se que a glória desta descoberta é do farmacêutico brasileiro Ezequiel Corrêa dos Santos e desta maneira, a pereirina é o primeiro alcaloide isolado no Brasil. As cascas do Pau-pereira foram, durante dois séculos, um importante remédio no combate a diversas doenças, devido às propriedades terapêuticas dos alcaloides presentes nas cascas da espécie e atualmente pesquisas estão sendo realizadas para comprovação científica de sua eficácia no combate a doenças virais como Aids, herpes e hepatite C.
This article describes the history of pereirina, an alkaloid extracted from Geissospermum vellosii Allemão barks, a Brazilian plant of Apocynaceae's family, popularly known as "pau-pereira". The botanical classification of this species and the use of pereirina in popular medicine were reasons for controversies and irritated disputes for the glory of the discovery among Brazilians and foreigners pharmacists. For two centuries, the pau-pereira's barks were important remedy to combat various diseases, because the therapeutic properties of alkaloids present in the barks of the species. The currently researches are being carried out for scientific proof of its effectiveness in combating viral diseases such as AIDS, herpes and hepatitis C.
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Este trabalho reproduz e comenta importante fonte primária muito citada nos estudos em história da saúde no Brasil, mas pouco conhecida em sua integralidade. Trata-se do discurso do médico Miguel Pereira, proferido em outubro de 1916, e no qual se referiu à zona rural do país como 'um imenso hospital'. A interpretação consagrada dessa fonte é a de que sua repercussão teve persistente e decisiva influência em políticas públicas de saúde na primeira metade do século XX. Neste texto, aprofundo o conhecimento das circunstâncias históricas que geraram a elaboração dessa expressão-síntese dos 'males do Brasil', sobretudo no que se refere às discussões, então em curso, a respeito do recrutamento militar obrigatório e do Exército como 'escola de civismo'.
The article reproduces and comments on an important primary source that is often cited in studies on Brazil's history of health but which few are familiar with in its entirety: the October 1916 speech by physician Miguel Pereira, in which he referred to rural Brazil as an "enormous hospital." The standard interpretation is that this source had a steady, decisive influence on public health policies in the first half of the twentieth century. In this text, I endeavor to deepen our knowledge of the historical circumstances which produced this expression that synthesized the 'evils of Brazil', especially in regard to the era's discussion of mandatory military recruitment and of the army as a 'civics school'.
Assuntos
Humanos , Médicos , Zona Rural , Saúde Pública/história , Discurso , Política de Saúde/história , Saneamento Rural , Brasil , Doença de Chagas , História do Século XXRESUMO
The aim of this work was to evaluate the genotypic fidelity of pear shoots (Pyrus communis L.) cultivar Carrick regenerated in vitro, using RAPD markers. Genomic DNA was extracted from leaves regenerated from pear shoots submitted to different treatments and from micropropagated matrix plants (control plant), using the protocol descripted by FERREIRA & GRATTAPAGLIA (1996). For primer selection, the Kits OPAN, OPA, and OPF (Operon Technologies, Inc.) were used. Seven primers were chosen: OPAN-03, OPAN-14, OPAN-15, OPAN-16, OPA-02, OPA-08 and OPF-04. Amplified products were submitted to horizontal electrophoresis in 1.2% agarose gel, stained with ethidium bromide and visualized under UV light. The result was documented by using a Polaroid Camera. The absence or addition of bands, compared to the control plant pattern was considered somaclonal variation. The seven primers generated 66 fragments with 100% monomorphics bands indicating that none of the primers used was able to detect somaclonal variation on the regenerated shoots.
O objetivo deste trabalho foi avaliar a fidelidade genotípica de brotações de pereira (Pyrus communis L.) cultivar Carrick, regeneradas in vitro, utilizando marcadores RAPDs. O DNA genômico foi extraído de folhas oriundas das brotações de pereira regeneradas a partir de diferentes tratamentos e de plantas matrizes micropropagadas (planta controle), utilizando-se o protocolo descrito por FERREIRA & GRATTAPAGLIA (1996). Para triagem dos primers foram utilizados os kits OPAN, OPA e OPF (Operon Technologies, Inc.) e, destes, foram escolhidos sete primers: OPAN-03, OPAN-14, OPAN-15, OPAN-16, OPA-02, OPA-08 e OPF-04. A separação dos produtos da amplificação foi realizada através de eletroforese horizontal em gel de agarose 1,2%, corado com brometo de etídio. Após a corrida, os géis foram visualizados sobre um transiluminador de luz ultravioleta e fotografados com câmara Polaroid para registro dos dados. A ausência ou adição de uma ou mais bandas comparativamente ao padrão da planta matriz (planta controle) foi considerado variação somaclonal. Dos 66 fragmentos produzidos pelos sete primers, observou-se 100% de bandas monomórficas, indicando que nenhum dos primers utilizados detectou variação somaclonal nas brotações regeneradas.
RESUMO
The aim of this work was to evaluate the genotypic fidelity of pear shoots (Pyrus communis L.) cultivar Carrick regenerated in vitro, using RAPD markers. Genomic DNA was extracted from leaves regenerated from pear shoots submitted to different treatments and from micropropagated matrix plants (control plant), using the protocol descripted by FERREIRA & GRATTAPAGLIA (1996). For primer selection, the Kits OPAN, OPA, and OPF (Operon Technologies, Inc.) were used. Seven primers were chosen: OPAN-03, OPAN-14, OPAN-15, OPAN-16, OPA-02, OPA-08 and OPF-04. Amplified products were submitted to horizontal electrophoresis in 1.2% agarose gel, stained with ethidium bromide and visualized under UV light. The result was documented by using a Polaroid Camera. The absence or addition of bands, compared to the control plant pattern was considered somaclonal variation. The seven primers generated 66 fragments with 100% monomorphics bands indicating that none of the primers used was able to detect somaclonal variation on the regenerated shoots.
O objetivo deste trabalho foi avaliar a fidelidade genotípica de brotações de pereira (Pyrus communis L.) cultivar Carrick, regeneradas in vitro, utilizando marcadores RAPDs. O DNA genômico foi extraído de folhas oriundas das brotações de pereira regeneradas a partir de diferentes tratamentos e de plantas matrizes micropropagadas (planta controle), utilizando-se o protocolo descrito por FERREIRA & GRATTAPAGLIA (1996). Para triagem dos primers foram utilizados os kits OPAN, OPA e OPF (Operon Technologies, Inc.) e, destes, foram escolhidos sete primers: OPAN-03, OPAN-14, OPAN-15, OPAN-16, OPA-02, OPA-08 e OPF-04. A separação dos produtos da amplificação foi realizada através de eletroforese horizontal em gel de agarose 1,2%, corado com brometo de etídio. Após a corrida, os géis foram visualizados sobre um transiluminador de luz ultravioleta e fotografados com câmara Polaroid para registro dos dados. A ausência ou adição de uma ou mais bandas comparativamente ao padrão da planta matriz (planta controle) foi considerado variação somaclonal. Dos 66 fragmentos produzidos pelos sete primers, observou-se 100% de bandas monomórficas, indicando que nenhum dos primers utilizados detectou variação somaclonal nas brotações regeneradas.