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The hypothesis was tested that the performance of lactating ewes is affected by the supplementation level and pasture management. Two supplementation levels (0.5 and 1.0% of body weight, BW) and two pasture managements (mowed and non-mowed) were tested. Forty adult ewes (2 years old) with an average weight at lambing of 62.97 ± 7.0 kg (day 0) and an average the body condition score of 2.5 points (day 0) were evaluated. Verminosis was monitored with periodic deworming. The number of eggs per gram of feces (EPG) of ewes at lambing was used as a covariate for performance assessments. Ewes lost an average of 7.5 kg over the 90 days post-partum (12% BW). The weaning rate was 53%. The body condition of the ewes was influenced by the post-partum period. The ewes mobilized their body reserves in the first 30 days of lactation. Mowing management negatively affected the nutritive value of the forage. Supplementation with 0.5% BW was sufficient for nutritional management post-partum. Pasture management (mowed vs. not mowed) cannot prevent post-partum weight loss. Supplementation levels and pasture management altered the morphological and chemical components of the pasture.

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The aim was to evaluate the animal response and the chemical and physical changes of free-choice mineral mixtures fed to grazing cattle. Growing beef cattle were fed either powder (POW) or agglomerated (AGL) mineral mixtures in three different experiments (Exp.), carried out in pastures of Brachiaria grass. In Exp. 1 and 2, the mineral mixtures were disposed in unsheltered troughs (POWun vs. AGLun), being delivered once (D0, Exp.1) or twice (D0 and D8, Exp. 2), throughout 14-day periods. In Exp. 3, POWun and AGLun were additionally compared to POW in sheltered troughs (POWshe), and the mineral mixtures were disposed in D0, throughout 21-day periods. Non-consumed supplement was removed and sampled on D14 (Exp. 1 and 2) or D21 (Exp. 3). Evaluations included average daily body weight gain (ADG), daily disappearance of the supplement (DSD), penetration force of the supplement mass, faecal chemical composition and serum levels of Ca, P and Mg. In Exp. 1, no effects were observed on ADG and faecal mineral concentrations, however, changes in mineral concentrations and a 40% reduction in Na concentration in the supplement were observed, compared to the initial concentration. AGLun had a lower penetration force. In Exp. 2, there were no effects on DSD and faecal mineral concentrations. POWun showed a smaller reduction in Na content compared to AGLun, and AGLun showed lower penetration force. In Exp. 3, the treatments did not affect ADG, but there was a trend towards higher DSD and serum phosphorus (P) concentration for AGLun (p = 0.08). Higher faecal Na concentration was observed for AGLun and higher Na concentration occurred in non-consumed mixture of POWshe. Mineral supplements offered in uncovered troughs showed altered chemical and physical characteristics, with possible effects on supplement intake. However, the general changes are unlikely to alter animal performance.

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Soybean-maize are cultivated in different management systems, such as no-tillage and pastures, which presents potential to add organic residues, and it can potentially impacts the soil microbial community present in these systems. Thus, this study aimed to examine the effects of different soybean-maize management practices on the diversity and composition of soil microbial communities. Specifically, 16 S rRNA amplicon sequencing was used to investigate whether the use of pasture species in a fallowing system influences microbial communities in a soybean-maize rotation system, as compared to conventional tillage and no-tillage systems. The results indicate that the inclusion of the pasture species Urochloa brizantha in soybean-maize management systems leads to distinct responses within the soil microbial community. It was found that different soybean-maize management systems, particularly those with U. brizantha, affected the microbial community, likely due to the management applied to this pasture species. The system with 3 years of fallowing before soybean-maize showed the lowest microbial richness (∼2000 operational taxonomic units) and diversity index (∼6.0). Proteobacteria (∼30%), Acidobacteria (∼15%), and Verrucomicrobia (∼10%) were found to be the most abundant phyla in the soil under tropical native vegetation, while soils under cropland had an increased abundance of Firmicutes (∼30% to ∼50%) and Actinobacteria (∼30% to ∼35%). To summarize, this study identified the impacts of various soybean-maize management practices on the soil microbial community and emphasized the advantages of adding U. brizantha as a fallow species.

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To gain insights into the forage morphological and anatomical characteristics in a silvopastoral system (SPS) with Bolsa de Pastor (Zeyheria tuberculosa) and palisadegrass 'Marandu' (Urochloa brizantha) monoculture (MONO). The SPS was established through natural regeneration of the tree species. Treatments were a SPS and MONO distributed in a completely randomized design with six replicates and repeated measures were the harvest periods. Response variables were morpho-physiological and anatomical characteristicss: green: dead material ratio, leaf blade: stem+sheath ratio, leaf area index, chlorophyll and carotenoid concentrations, proportions of non-lignified and achlorophyllous areas, lignified areas in stems, proportions of non-lignified and achlorophyllous areas, lignified and chlorophyllous areas in leaves, as well as cell length in longitudinal section of stem. Morpho-physiological patterns were altered (p < 0.05) under natural shading conditions due to higher photosynthetic efficiency in the SPS. There was no effect (p > 0.05) of the systems on anatomical patterns, proportions of non-lignified and achlorophyllous, lignified and chlorophyllous tissues, these proportions were influenced only by the periods of the year, both for stems and leaves. Cells of the internodes of the grasses of the studied systems had the same length. The SPS alters morpho-physiological characteristics of palisadegrass and increases the concentration of chlorophyll a and b.(AU)

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The introduction of a forage legume into a tropical pasture should decrease the need for N fertilizer, provided biological N2 fixation (BNF) contributes enough to compensate for exported N. Macrotyloma axillare (perennial horsegram) is a suitable legume for composing mixed pastures, and our hypothesis is that the isolation of indigenous rhizobia from roots and rhizosphere is the way of achieving an efficient inoculant to maximize BNF to the legume. Nodules and rhizosphere soil taken from M. axillare grown in a mixed pasture with palisade grass were sampled and used in a trap host assay using Leonard jars containing a mixture of vermiculite and sand. A total of ten bacteria were initially isolated using this technique. The isolates were then used in two experiments to evaluate the inoculation responses on the perennial horsegram in greenhouse conditions to which nodulation, plant growth, and shoot N accumulation were measured. Phylogenetic analyses based on 16S rRNA and recA placed all strains within genus Bradyrhizobium, some of them not previously described. The best strain provided more than 120 nodules and more than 65 mg of nodules per plant. Strain BR14182 was considered the most promising given the high dry matter and N accumulation in plant shoots. This study provides the first analysis of Bradyrhizobium diversity nodulating M. axillare in Brazil and provided evidence of the role of inoculation in incrementing the plant-rhizobium symbiosis in a forage legume.

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The objective of this study was to investigate the influence of timing of paddock allocation (AM or PM) in tropical grass on nutritive value of the herbage, dry matter intake (DMI), milk yield and composition, ruminal fermentation, nitrogen excretion, and enteric CH4 emissions of dairy cows. Twenty cows were grouped in pairs and randomly distributed within pair to one of two treatments. PM herbage had greater contents of dry matter (DM), soluble carbohydrates, starch, and nonfibrous carbohydrate to protein ratio and lower contents of neutral detergent fiber and acid detergent fiber. There was no treatment effect on DMI, N excretion, milk yield, and CH4 emissions. However, milk protein and casein yields tended to be greater for PM than AM, while milk urea nitrogen concentration was lower for PM than AM. The increase in nutritive value of the afternoon relative to the morning herbage within the framework of this study was not large enough to increase DMI and milk yield or to decrease CH4 emission intensity by the dairy cows as hypothesized. The findings indicate that PM treatment can be a simple and useful grazing strategy that results in an herbage harvest with greater nutritional value and in lower excretion of urea N into milk.

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This study examined the effects of annual nitrogen (N) rates of 100 and 200 kg ha-¹ on the morphogenetic and structural traits of Ipyporã grass (Brachiaria brizantha × Brachiaria ruziziensis) under intermittent grazing during the seasons of the year. The experiment was laid out in a randomized-block design with two treatments and three area replicates. Ipyporã grass pastures were grazed by beef cattle. Morphogenetic and structural traits of the forage canopy were evaluated using the marked-tiller technique. Forage accumulation and tiller density (TD) were also evaluated. Significant interaction effects (P < 0.05) between N rates and seasons were observed for the rest period, leaf lifespan (LLS), phyllochron, stem elongation rate (SER) and final leaf length (FLL). During winter, the rest period was shorter (40 vs. 50 days) in the pastures fertilized with 200 kg ha-¹ N. Conversely, in winter, the pastures fertilized with 100 kg ha-¹ N showed higher LLS, SER and phyllochron values, but lower FLL values. There was no isolated effect of N rates (P > 0.05) on forage accumulation rate; leaf appearance, elongation or senescence rates; leaf:stem ratio; or TD. However, the effect of seasons was significant for these variables, with the highest values occurring during spring and summer. The use of 200 kg ha-¹ N in Ipyporã grass pastures reduces the seasonality of forage production.

O objetivo foi avaliar os efeitos das doses anuais de 100 e 200 kg ha-¹ de nitrogênio (N) sobre as características morfogênicas e estruturais do capim-ipyporã, em lotação intermitente durante as estações do ano. O delineamento experimental foi em blocos ao acaso, com dois tratamentos e três repetições de área. Os pastos de capim-ipyporã foram submetidos ao pastejo por bovinos de corte. Foram avaliadas as características morfogênicas e estruturais do dossel forrageiro por meio da técnica de perfilhos marcados. Também foi avaliado o acúmulo de forragem e a densidade populacional de perfilhos (DPP). Foi observada interação significativa (P < 0,05) das doses de N e estações do ano para o período de descanso (PD), duração de vida das folhas (DVF), filocrono, taxa de alongamento de colmos (TAlC) e comprimento final das folhas (CFF). Durante o inverno, o PD foi menor (40 vs 50 dias) nos pastos adubados com 200 kg ha-¹ de N. Em contra partida, no inverno, os pastos adubados com 100 kg ha-¹ de N apresentaram maiores valores para DVF, filocrono e TAlC, e menores para o CFF. Não foi observado efeito isolado de doses de N (P > 0,05) para as taxas de acúmulo de forragem; de aparecimento, alongamento e de senescência de folhas, relação folha:colmo e para a DPP. No entanto, o efeito das estações foi significativo para estas variáveis, com os maiores valores observados durante a primavera e verão. O uso de 200 kg ha-¹ de N para pastos de capim-ipyporã promove redução da estacionalidade de produção da forrageira.

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This study evaluated the effect of increased energy via supplementation on the performance, ingestive behavior, nutrient digestibility, and nitrogen metabolism of grazing heifers fed tropical forage in the rainy-dry transition season. Treatments consisted of mineral supplementation ad libitum (control) and multiple supplements formulated to provide different energy levels and the same amount of protein (300 g CP animal d-1) and were denominated as low (LE; 340 g TDN animal d-1), medium (ME; 780 g TDN animal d-1) and high (HE; 1220 g TDN animal d-1) energy. Animals supplemented with ME, and HE had a greater average daily gain in relation to the control treatment, with an increase of 41 and 46%, respectively. Greater values for total apparent digestibility of neutral detergent fiber were observed for the treatment HE. Lesser values of urinary urea N were observed for the control and HE treatments. Our results define the use of energy levels in the supplement as a tool for pasture management. If the purpose of the production system is to enhance forage intake, the option is to supply supplements with less energy levels. In contrast, if the purpose is to increase the stocking rate, supplements with greater energy levels should be used.(AU)

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A infestação de capim-annoni (Eragrostis plana Ness) nas áreas de pastejo em campo nativo ameaça a preservação do Bioma Pampa e a atividade pecuária no Rio Grande do Sul (RS). O objetivo deste trabalho foi avaliar a influência de tratamentos de adubação e calagem sobre a competitividade de espécies forrageiras do campo nativo com o capim-annoni, em área infestada pela invasora. Foi realizado um experimento em área de campo nativo infestada com capim-annoni no Centro de Pesquisa do DDPA/SEAPDR em Viamão - RS, sem uso de herbívoros domésticos, para avaliação do efeito dos tratamentos sem interferência do pastejo seletivo. Foram avaliados quatro tratamentos: TO sem adubação e calagem: T1 ­ apenas calagem: T2 - com adubação (nitrogênio, fósforo e potássio) e calagem; T3 - adubação com o dobro da dose de fósforo, mais potássio e calagem. Foram acompanhadas a evolução da composição botânica e o desenvolvimento das espécies do campo nativo e do capim-annoni por dois anos, pela avaliação da cobertura vegetal do solo e da produção de massa seca, como também as modificações da fertilidade do solo. Os resultados de produção de massa seca mostraram que a adubação nitrogenada do tratamento T2 beneficiou o crescimento do campo nativo e desfavoreceu o capim-annoni. Com a adubação nitrogenada do tratamento T2, espécies de gramíneas cespitosas do campo nativo atingiram maior altura e provavelmente competiram com o capim-annoni por meio de sombreamento, resultando na redução do crescimento da invasora.

The invasion of tough lovegrass (Eragrostis plana Ness) in native grassland grazing areas threatens the preservation of the Pampa biome as well as livestock farming in Rio Grande do Sul (RS), Brazil. The objective of the present study was to evaluate the influence of fertilization and liming treatments on the competitiveness of native grassland forage species with tough lovegrass, in an area infested by the invader. An experiment was carried out in a native grassland area infested with tough lovegrass that was not subjected to domestic herbivores at the DDPA/SEAPDR Research Center in Viamão - RS, to evaluate the effects of the treatments without interference from selective grazing. The following four treatments were assessed: TO - no fertilization or liming: T1 - liming only; T2 - fertilization (nitrogen, phosphorus and potassium) and liming; and T3 - fertilization with double the phosphorus dose, plus potassium and liming. The evolution of botanical composition and development of native grassland species and tough lovegrass were monitored for two years, by evaluating soil vegetal coverage and dry mass production, as well as modifications of soil fertility. The dry mass production results showed that nitrogen fertilization from T2 treatment benefited the growth of native grassland species and disfavored tough lovegrass. With nitrogen fertilization from T2 treatment, native grassland tufted grass species grew taller and probably competed with tough lovegrass through shading, resulting in reduced growth of the invader.

La infestación del capim annoni (Eragrostis plana Ness) en áreas de pastoreo en pastizales nativos amenaza la preservación del Bioma Pampa y la actividad ganadera en Rio Grande do Sul (RS), Brasil. El objetivo de este trabajo fue evaluar la influencia de los tratamientos de fertilización y encalado sobre la competitividad de las especies forrajeras del campo nativo con el capim annoni, en un área infestada por el invasor. Se realizó un experimento en un área de campo nativo infestado con capim annoni en el Centro de Investigación del DDPA/SEAPDR en Viamão - RS, sin el uso de herbívoros domésticos, para evaluar el efecto de los tratamientos sin la interferencia del pastoreo selectivo. Se evaluaron cuatro tratamientos: TO sin fertilización y encalado; T1 sólo encalado; T2 con fertilización (nitrógeno, fósforo y potasio) y encalado; T3 - fertilización con doble dosis de fósforo, más potasio y encalado. La evolución de la composición botánica y el desarrollo de las especies del campo nativo y del capim annoni fueron seguidos durante dos años, mediante la evaluación de la cobertura vegetal del suelo y la producción de masa seca, así como las modificaciones en la fertilidad del suelo. Los resultados de la producción de masa seca mostraron que la fertilización nitrogenada del tratamiento T2 benefició el crecimiento del campo nativo y perjudicó al capim annoni. Con la fertilización nitrogenada del tratamiento T2, las especies gramíneas cespitosas del campo nativo alcanzaron una mayor altura y probablemente compitieron con el capim annoni mediante el sombreado, lo que resultó en la reducción del crecimiento de la planta invasora.

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<b>Background and Objective:</b> Forage production in the tropics is generally asymmetrically distributed. Hence the need to use more complex models, especially when multiple comparisons are made and there are very large deviations from normality. The objective of this research is to fit a Generalized Additive Model for Location, Scale and Shape (GAMLSS) model on accumulated dry matter data from <i>Brachiaria brizantha</i> using a model selection algorithm. <b>Materials and Methods:</b> A Box-Cox Power Exponential (BCPE) distribution was adjusted on the dry matter from <i>Brachiaria brizantha</i> data implementing GAMLSS in R (programming language). The accumulated dry matter data for <i>B. brizantha</i> were obtained from a study carried out on a farm in the state of Portuguesa, Venezuela. The explanatory covariate x was the interval between cuts (21, 28, 35 and 42 days). <b>Results:</b> The dependent variable (dry matter) exhibited both skewness and kurtosis. GAMLSS allowed flexible modeling of both the distribution of the dry matter yield from <i>B. brizantha</i> and the dependence of all the parameters of the distribution on intervals between cuttings. For the dry matter yield from <i>B. brizantha</i>, which exhibited skewness and leptokurtosis, the BCPE distribution, provided the best fit. <b>Conclusion:</b> The interval between cuttings showed an effect that is reflected in the average yield of dry matter from <i>B. brizantha</i>. The interval between cuts affected the skewness and the kurtosis of the distribution.

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Soils provide a broad set of vital ecosystem services and sustains the production of food and fibers, balancing the ecosystem. Thus, from the perspective of soil quality, it is defined as an ability to balance within the ecosystem to sustain biological productivity, promoting the health of plants and animals, being evaluated by traditional indicators as physical, chemical and biological indicators, so the present work aims to estimate the soil quality index using multivariate models using soil biological attributes and validation with growth variables of the bioindicator plant. The study was developed in the agricultural area in P. Prudente, SP, the points collected were georeferenced, collections in depth of 0 -20 cm, microbiological analysis, microbial carbon and nitrogen biomass, dehydrogenase, respiration and microbial coefficient, having a bioindicator plant curly lettuce (Lucy Brown) as a validator of the soil. The results were discovered using the PCA model for the identification of autos vectors and autos values, grouping and identifying their collinearities, linear regression, r-pearson validation and cluster heuristic analysis. The microbial attributes and the bioindicator plant discriminated the agricultural areas evaluated with establishment and validation of SQI. The metabolic coefficient and N of the microbial biomass dissipation of the highest covariance values by multivariate analysis. The reforestation area with native species (SQI0.782%) and the livestock crop integration system (SQI0.765%) were evaluated as areas with better soil quality.

O solo fornece o conjunto de serviços ambientais vitaise dinâmico que condiciona e sustenta a produção de alimentos e fibras, balanceando o ecossistema, dessa forma, sob a ótica da qualidade do solo é definida como a capacidade de se equilibrar dentro do ecossistema para sustentar a produtividade biológica, promovendo a saúde das plantas e animais, sendo avaliada pelos indicadores tradicionais como indicadores físicos, químicos e biológicos, assim o presente trabalho tem como objetivo estimar índice qualidade do solo com uso de modelos multivariados utilizando-se de atributos biológicos do solo e validação com variáveis de crescimento da planta bioindicadora. O estudo foi desenvolvido na área agrícola da universidade do Oeste Paulista, campus II, os pontos coletados foram georreferenciados, coletatos em profundidade de 0 –20 cm, realizado as análises microbiológicas, biomassa microbiana do carbono e nitrogênio, desidrogenase, respiração e coeficiente microbiano, tendo a planta bioindicadora alface crespa (Lucy Brown) como validador do solo. Os resultados foram submetidos ao modelo de PCA para a identificação dos autos vetores e autos valores agrupando e identificando suas colinearidades, regressão linear, validação r-pearson e análise heurística de cluster. Os atributos microbianos e a planta bioindicadora discriminaram as áreas agrícolas avaliadas com atribuição e validação de SQI. O coeficiente metabólico e N da biomassa microbiana apresentaram os maiores valores de covariância pela análise multivariada. A área de reflorestamentocom espécies nativas (SQI0,782%) e o sistema de integração lavoura pecuária (SQI0,765%) foram consideradas as áreas com melhor qualidade de solo.

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Soils provide a broad set of vital ecosystem services and sustains the production of food and fibers, balancing the ecosystem. Thus, from the perspective of soil quality, it is defined as an ability to balance within the ecosystem to sustain biological productivity, promoting the health of plants and animals, being evaluated by traditional indicators as physical, chemical and biological indicators, so the present work aims to estimate the soil quality index using multivariate models using soil biological attributes and validation with growth variables of the bioindicator plant. The study was developed in the agricultural area in P. Prudente, SP, the points collected were georeferenced, collections in depth of 0 -20 cm, microbiological analysis, microbial carbon and nitrogen biomass, dehydrogenase, respiration and microbial coefficient, having a bioindicator plant curly lettuce (Lucy Brown) as a validator of the soil. The results were discovered using the PCA model for the identification of autos vectors and autos values, grouping and identifying their collinearities, linear regression, r-pearson validation and cluster heuristic analysis. The microbial attributes and the bioindicator plant discriminated the agricultural areas evaluated with establishment and validation of SQI. The metabolic coefficient and N of the microbial biomass dissipation of the highest covariance values by multivariate analysis. The reforestation area with native species (SQI0.782%) and the livestock crop integration system (SQI0.765%) were evaluated as areas with better soil quality.(AU)

O solo fornece o conjunto de serviços ambientais vitaise dinâmico que condiciona e sustenta a produção de alimentos e fibras, balanceando o ecossistema, dessa forma, sob a ótica da qualidade do solo é definida como a capacidade de se equilibrar dentro do ecossistema para sustentar a produtividade biológica, promovendo a saúde das plantas e animais, sendo avaliada pelos indicadores tradicionais como indicadores físicos, químicos e biológicos, assim o presente trabalho tem como objetivo estimar índice qualidade do solo com uso de modelos multivariados utilizando-se de atributos biológicos do solo e validação com variáveis de crescimento da planta bioindicadora. O estudo foi desenvolvido na área agrícola da universidade do Oeste Paulista, campus II, os pontos coletados foram georreferenciados, coletatos em profundidade de 0 –20 cm, realizado as análises microbiológicas, biomassa microbiana do carbono e nitrogênio, desidrogenase, respiração e coeficiente microbiano, tendo a planta bioindicadora alface crespa (Lucy Brown) como validador do solo. Os resultados foram submetidos ao modelo de PCA para a identificação dos autos vetores e autos valores agrupando e identificando suas colinearidades, regressão linear, validação r-pearson e análise heurística de cluster. Os atributos microbianos e a planta bioindicadora discriminaram as áreas agrícolas avaliadas com atribuição e validação de SQI. O coeficiente metabólico e N da biomassa microbiana apresentaram os maiores valores de covariância pela análise multivariada. A área de reflorestamentocom espécies nativas (SQI0,782%) e o sistema de integração lavoura pecuária (SQI0,765%) foram consideradas as áreas com melhor qualidade de solo.(AU)

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus – ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

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The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

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The work herein reported closes the evaluation of the role of kikuyu grass in small-scale dairy systems in the highlands of Mexico. The objective was to compare the productive response of vacas lecheras en pastoreo continuo de kikuyu (Cenchrus clandestinus) with a sown frost-resistant tall fescue (Lolium arundinaceum) during the winter-spring dry season in dairy systems and determine the fatty acid profile of feeds and milk. An on-farm double cross-over experiment with three periods the 14 days each was undertaken with eight Holstein cows randomly assigned to treatments sequence. Treatments were daytime grazing for 8 h/d of a Cajun II endophyte free tall fescue pasture invaded by kikuyu grass (CJ) or a naturally invaded kikuyu grass pasture (KY), both associated with white clover (Trifolium repens) and annual ryegrass (Lolium multiflorum). Cows were supplemented in pens with 6.0 kg DM/cow/day with maize silage and 4.6 kg DM/cow/day of commercial concentrate. The fatty acid profiles of feeds and milk were determined by gas chromatography. There were differences (P<0.05) for net herbage accumulation and chemical composition between pastures, but not for in vitro digestibility or estimated metabolizable energy. In animal variables, protein content in milk was higher in KY (P<0.05). There were significant differences (P<0.05) among experimental periods for milk fat content and milk urea nitrogen with the highest values in Period 3. Pasture DM intake was lowest (P<0.05) in Period 3. In terms of fatty acid content, there were significant interactions (P<0.05) for vaccenic acid (C18:1t11) and linoleic acid (C18:2c9c12) with the highest values in Period 3. Linolenic acid (C18:3c9c12c15) was higher in milk when cows grazed KY and significantly higher (P<0.05) in Period 3. It is concluded that kikuyu pastures complemented with maize silage and concentrates in winter-spring perform as tall fescue pastures in the season of herbage scarcity. Milk from cows grazing kikuyu grass pastures complemented with maize silage and concentrates has a higher content of linolenic fatty acid and an atherogenic index favorable for human health.

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Grazing in small-scale dairy systems reduces costs and enhances sustainability. However, binary pastures (BP) have low persistency, which may be increased by including more species given their flexibility to withstand different agroecological and management situations. The objective was to assess a multi-species (MS) pasture of perennial ryegrass (RG), tall fescue (TF), bromegrass (BG), red clover (RC) and white clover (WC) in comparison to a BP of perennial ryegrass (RG) - WC grazed by six Holstein dairy cows during the dry season in an on-farm double cross-over experiment, with three 14-day each experimental periods for animal variables, and a split-plot design for pasture variables at 3 cow/ha. There were differences (P < 0.05) between pastures for sward height (MS 5.0 vs. BP 4.0 ± 0.10 cm, both MS and BP) and net herbage accumulation (MS 902 vs BP 228 ± 172.4 kg DM/ha, both MS and BP) and differences (P < 0.05) for chemical composition among periods. There were differences (P < 0.01) among periods for estimated DM intake, but no differences (P > 0.05) for milk yield (mean 16.8 kg/cow/day), milk fat or protein content (mean 31.8 and 28.8 g/kg). Multi-species pastures are a viable alternative for SSDS in the highlands of central Mexico, being more resistant to harsh environment and to weed and kikuyu grass invasion.

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As plantas tóxicas causam grandes prejuízos econômicos para a pecuária em diversas regiões do Brasil, provocando, até mesmo, a morte de animais. Poucas são as informações disponíveis sobre as intoxicações por plantas em bovinos, no estado de Minas Gerais. O estudo sistemático das espécies tóxicas, em regiões com poucas pesquisas, aumenta, consideravelmente, os dados sobre plantas tóxicas de interesse pecuário. Sendo assim, o objetivo deste estudo foi realizar um levantamento das plantas tóxicas para bovinos nas propriedades rurais do município de São João Evangelista, Minas Gerais. Para isso, foram visitadas, aleatoriamente, 20propriedades rurais do município e três formulários foram aplicados aos produtores rurais, sob a forma de entrevista, sendo que as informações obtidas, por meio dos formulários, foram analisadas quanti e qualitativamente. Também, foram coletadas amostras de solos para análise das características químicas do local incidente da planta tóxica. Com a análise, observou-se que a incidência de plantas tóxicas para bovinos atinge55% das propriedades rurais e em 60% das propriedades há histórico de casos de intoxicação em bovinos. Seis espécies de plantas tóxicas incidem nas pastagens das propriedades rurais, a Pteridium aquilinum, a Palicourea marcgravii, a Lantana camara, a Asclepias curassavica, a Ricinus communis e a Palicourea grandiflora. A espécie Pyrostegia venusta foi relatada como planta tóxica para bovinos e a P. marcgravii associada à maior quantidade de mortes de bovinos no município. Observou-se também, que as espécies de plantas tóxicas possuem a capacidade de desenvolvimento em condições de acidez do solo e de baixa fertilidade e em pastagens bem manejadas com boa fertilidade.

Toxic plants cause important economic damage to livestock in several regions of Brazil, even causing the death of animals. Little information is available about plant poisoning in cattle in the State of Minas Gerais. The systematic study of toxic species in regions with little research considerably increases the data on toxic plants of livestock interest. The objective of this study was to carry out a survey of toxic plants for cattle in rural properties in the municipality of São João Evangelista, Minas Gerais. Twenty rural properties in the municipality were visited at random. Three forms were applied to rural producers in the form of an interview. The information obtained through the forms were analyzed quantitatively and qualitatively. Soil samples were collected to analyze the chemical characteristics of the incident site of the toxic plant. The incidence of toxic plants for cattle affects 55% of rural properties and in 60% of properties there is a history of cases of intoxication in cattle. Six species of toxic plants affect the pastures of rural properties, being Pteridium aquilinum, Palicourea marcgravii, Lantana camara, Asclepias curassavica, Ricinus communis and Palicourea grandiflora. The species Pyrostegia venusta has been reported as a toxic plant for cattle. Palicourea marcgravii is associated with the highest number of cattle deaths in the municipality. Toxic plant species the capacity to establish themselves in conditions of soil acidity and low fertility, and in well-managed pastures with good fertility.

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Climate change (CC) affects food production, mainly those based on livestock systems. Producers must identify adaptation strategies to ensure the production, during periods of drought, and lack of forage. Besides contributing to CC, high emissions of ruminal methane (CH4) are energy loss potentially usable for livestock production. The objective was to estimate in vitroruminal gas production (RGP) and determine the CH4emissions from silages. Treatments were made with forage of Cenchrus purpureusmixed with Tithonia diversifoliaT1= C.purpureusat 100%; T2= C.purpureus/ T.diversifoliain 33/67 percent ratio; T3= C.purpureus/ T.diversifolia67/33; and T4= T.diversifoliaat 100%. Samples of silages were analyzed, andthey were inoculated with strains of Lactobacillus paracasei(T735); then they were fermented in vacuum-sealed bags for 67 days. RGP and CH4 were measured at 2, 4, 8, 12, 18, 24, 30, 36, and 48 hours. Additionally, modeling of CH4 production kinetics was conducted, using different equations. The results indicate that the highest cumulative CH4 production was for T1. This kinetics was represented using the Gompertz model. In conclusion, the inclusion of T.diversifoliato C.purpureussilages contributes to the decrease of methane at the ruminal level, which constitutes an adaptation practice at climate change.

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Hypomagnesemia is a major cause of death in grazing beef cows in countries of the Southern Hemisphere such as Argentina, Australia and New Zealand. Here we review the literature on hypomagnesemia in beef cattle and describe an outbreak in Uruguay. The disease occurred in late autumn, affecting 6 to 11-year-old Aberdeen Angus and Hereford x Aberdeen Angus lactating multiparous cows in good body condition, grazing on natural grasslands that had been improved by fertilization and sowing of seeds of ryegrass and oat. Approximately 40 out of 225 cows were affected and 24 (10.7%) died. A presumptive diagnosis of hypomagnesemia was established based on sudden death, acute neuromuscular clinical signs, epidemiological data, and the response to Mg administration. The diagnosis was confirmed by detecting low Mg concentrations in serum (0.47-0.57mmol/L), vitreous humor (0.47-0.80mmol/L), aqueous humor (0.66mmol/L) and cerebrospinal fluid (0.59mmol/L). The largest component of the diet corresponding to fast-growing and exuberant forages of ryegrass and oat had high concentrations of K (3.48%), N (4.38%) and P (0.94%), suggesting secondary hypomagnesemia. In addition, the K/(Ca+Mg) ratio was 2.38 in forages of ryegrass and oat (reference value: 2.2), and 0.15 in the soil (reference value: 0.09), which represent risk factors for hypomagnesemia. In conclusion, hypomagnesemia is an important cause of mortality in beef cattle grazing improved natural grasslands in Uruguay and it can be easily prevented by correct seasonal Mg supplementation.(AU)

A hipomagnesemia é uma das principais causas de morte em vacas de corte em pastagem em países do Hemisfério Sul, como Argentina, Austrália e Nova Zelândia. Aqui, revisamos a literatura sobre hipomagnesemia em bovinos de corte e descrevemos um surto no Uruguai. A doença ocorreu no final do outono, afetando vacas Aberdeen Angus e Aberdeen Angus x Hereford de 6-11 anos de idade em boas condições corporais, lactantes, multíparas e pastando em campos naturais que foram melhorados com fertilização e plantio de azevém e aveia. Aproximadamente 40 de 225 vacas foram afetadas e 24 (10,7%) morreram. O diagnóstico presuntivo de hipomagnesemia foi estabelecido com base na morte súbita, sinais clínicos neuromusculares agudos, dados epidemiológicos e a resposta à administração de Mg. O diagnóstico foi confirmado pela detecção de baixos níveis de Mg no soro (0,47-0,57mmol/L), humor vítreo (0,47-0,80mmol/L), humor aquoso (0,66mmol/L) e líquido cefalorraquidiano (0,59mmol/L). O componente mais importante da dieta correspondia a pastagens de azevém e aveia de rápido crescimento, as quais apresentaram altas concentrações de K (3,48%), N (4,38%) e P (0,94%), que sugerem hipomagnesemia secundária. Além disso, a relação K/(Ca+Mg) foi de 2,38 em forragens de azevém e aveia (valor de referência: 2,2) e 0,15 no solo (valor de referência: 0,09), que representam fatores de risco para hipomagnesemia. Em conclusão, a hipomagnesemia é uma causa importante de mortalidade em gado de corte em pastagens naturais melhoradas no Uruguai e pode ser facilmente prevenida pela correta suplementação sazonal de Mg.(AU)

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