RESUMO

Antimicrobial resistance (AMR) is one of the main global health challenges. Anaerobic digestion (AD) can significantly reduce the burden of antibiotic resistance genes (ARGs) in animal manures. However, the reduction is often incomplete. The agronomic use of digestates requires assessments of their effects on soil ARGs. The objective of this study was to assess the effect of digestate on the abundance of ARGs and mobile genetic elements (MGEs) in the rhizosphere of ryegrass (Lolium perenne L.) and to determine whether half-dose replacement of digestate with urea (combined fertilizer) can be implemented as a safer approach while maintaining a similar biomass production. A greenhouse assay was conducted during 190 days under a completely randomized design with two experimental factors: fertilizer type (unfertilized control and fertilized treatments with equal N dose: digestate, urea and combined fertilizer) and sampling date (16 and 148 days after the last application). The results indicated that the digestate significantly increased the abundance of clinical class 1 integrons (intI1 gene) relative to the unfertilized control at both sampling dates (P < 0.05), while the combined fertilizer only increased them at the first sampling. Sixteen days after completing the fertilization scheme only the combined fertilizer and urea significantly increased the biomass production relative to the control (P < 0.05). Additionally, by the end of the assay, the combined fertilizer showed significantly lower levels of the macrolide-resistance gene ermB than digestate and a cumulative biomass similar to urea or digestate. Overall, the combined fertilizer can alleviate the burden of integrons and ermB while simultaneously improving biomass production.

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Mini-roses (Rosa chinensis Jacq.) is largely used in salty dishes and desserts. This study evaluated instrumental color, sugars, organic acids, phenolics, volatiles, and the indigenous microbiota (fungi and bacteria) in edible mini-roses farmed in discarded fruits biocompost and animal manure systems. A descriptive sensory analysis of flowers was also performed. Mini-roses farmed in biocompost had higher luminosity and intensity of instrumental red color, a higher concentration of phenolic compounds, including anthocyanins related to red color, and fructose than mini-roses farmed in animal manure (p < 0.05). Furthermore, mini-roses farmed in biocompost had higher concentrations of various volatiles (p < 0.05), including hexyl acetate and cis-3 -hexenyl butyrate related to the fruity aroma. Bacterial groups related to plant growth-promoting such as Stenotrophomonas and endophilic fungal groups such as Eurotiales sp, Pleosporales sp were found in higher abundance (p < 0.05) in mini-roses farmed in biocompost. Mini-rose farmed in biocompost also received higher score (p < 0.05) for fruity aroma and red color than mini-rose mini-roses farmed in animal manure. Results indicate that farming mini-roses using biocompost from discarded fruits impacts the synthesis of phenolics and volatiles, resulting in a more intense fruity aroma and red color. Findings also suggest that the microbiota of mini-roses farmed in biocompost or animal manure do not represent a major risk for the safety of these products.

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Composted sewage sludge (CSS) is an organic fertilizer that can be used as a source of micronutrients in agriculture. However, there are few studies with CSS to supply micronutrients for the bean crop. We aimed to evaluate micronutrient concentrations in the soil and their effects on nutrition, extraction, export, and grain yield in response to CSS residual application. The experiment was carried out in the field at Selvíria-MS, Brazil. The common bean cv. BRS Estilo was cultivated in two agricultural years (2017/18 and 2018/19). The experiment was designed in randomized blocks with four replications. Six different treatments were compared: (i) four increasing CSS rates, i.e., CSS5.0 (5.0 t ha-1 of applied CSS, wet basis), CSS7.5, CSS10.0, CSS12.5; (ii) a conventional mineral fertilizer (CF); (iii) a control (CT) without CSS and CF application. The available levels of B, Cu, Fe, Mn, and Zn were evaluated in soil samples collected in the 0-0.2 and 0.2-0.4 m soil surface horizons. The concentration, extraction, and export of micronutrients in the leaf and productivity of common beans were evaluated. The concentration of Cu, Fe, and Mn ranged from medium to high in soil. The available levels of B and Zn in the soil increased with the residual rates of CSS, which were statistically not different from the treatments with CF. The nutritional status of the common bean remained adequate. The common bean showed a higher requirement for micronutrients in the second year. The leaf concentration of B and Zn increased in the CSS7.5 and CSS10.0 treatments. There was a greater extraction of micronutrients in the second year. Productivity was not influenced by the treatments; however, it was higher than the Brazilian national average. Micronutrients exported to grains varied between growing years but were not influenced by treatments. We conclude that CSS can be used as an alternative source of micronutrients for common beans grown in winter.

RESUMO

En Perú, al 2019, solo el 6,4 % de los distritos disponen sus residuos urbanos en rellenos sanitarios, los cuales, están en situación de colapso, lo que exige buscar alternativas para mejorar la gestión de estos residuos y, frente a ello, el compostaje se perfila como una tecnología simple y económica, empleada para el tratamiento del componente orgánico, para reducir daños a la salud y al ambiente, sin embargo, la falta de criterios técnicos en su aplicación está afectando la calidad del compost final. El objetivo de esta investigación fue evaluar los criterios técnicos de procesamiento y la calidad del compost, a través de residuos sólidos urbanos en los distritos de la provincia Leoncio Prado. La investigación identificó los distritos que aplican el compostaje a sus residuos y evaluó la técnica de procesamiento, indicadores fisicoquímicos y determinó la calidad con base en normas técnicas internacionales. Los resultados muestran que seis de diez distritos aplican el compostaje y los indicadores fisicoquímicos contrastados con los criterios de calidad de la norma técnica chilena (NCH 2880), colombiana 5167 y de la OMS, corresponden a compost de calidad intermedia (Clase B). Los compost evaluados no representan riesgo ambiental y pueden ser utilizados como enmienda en la mejora del suelo y los cultivos con algunas restricciones, por presentar valores elevados de humedad, pH y bajos niveles de P, Ca, Mg y K.

At 2019, in Peru, only 6.4 % of the districts dispose of their urban waste in sanitary landfills, which are in a situation of collapse, therefore is needed looking for alternatives to improve the management of this waste, facing this, composting is emerging as a simple and economical technology used for the treatment of the organic component, thus, reducing damage to health and the environment, however, the lack of technical criteria in its application is affecting the quality of the final compost. The objective of this research was to evaluate the technical criteria for processing and the quality of compost based on municipal solid waste in the districts of Leoncio Prado province. The research identified the districts that apply composting to their waste and evaluated the processing technique, physicochemical indicators and determined the quality based on international technical standards. The results show that six out of ten districts apply composting, and the physicochemical indicators contrasted with the quality criteria of the Chilean technical standard (NCH 2880), Colombian 5167 and WHO, correspond to intermediate quality compost (Class B). The evaluated compost does not represent an environmental risk and can be used as an amendment in soil and crop improvement with some restrictions, since it has high moisture, pH and low levels of P, Ca, Mg and K.

RESUMO

Livestock waste is widely used in agriculture. Although they provide benefits to the soil, and consequently to plants, they have the potential to contaminate the environment, as they contain pathogenic microorganisms and determinants of antimicrobial resistance, if not properly managed. Therefore, this study aims to evaluate the effect of composting horse bedding and poultry litter in organic and conventional production systems on the occurrence of bacteria in the Enterobacteriales order and to identify their antimicrobial resistance profiles. Bacterial strains were isolated from Salmonella-Shigella and eosin methylene blue solid media from animal waste during the composting process that was conducted for 125 days. After isolation, the strains were identified by the MALDI-TOF technique; the disk diffusion test was then performed for phenotypic detection of antimicrobial resistance. A total of 158 bacterial strains were isolated during composting of three wastes. The Enterobacteriaceae family was the most abundant, whereas Proteus mirabilis and Escherichia coli were the species with the highest percentage in the wastes, which also exhibited a multi-resistance profile. Poultry litter showed a greater abundance of resistant bacteria than horse bedding did. Similarly, a greater number of resistant bacteria was detected in conventional poultry litter than in organic poultry litter. The results obtained reinforce that animal wastes are reservoirs of pathogenic bacteria that are resistant to antimicrobials and highlight the importance of developing management strategies that aim to reduce and/or eliminate these contaminants to guarantee their safe use in agriculture.

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Background: This work studied the use of the organic fertilizers DTPA-Fe and EDDS-Fe as iron chelates for solar driven photo-Fenton process at natural pH. This process was proposed to investigate its performance on removing a mixture of agrochemicals (propiconazole, imidacloprid and diuron) from pineapple processing wastewater to obtain a suitable effluent to be reused in the agricultural sector. Methods: Experiments were carried out in a solar simulator with a stirred cylindric photoreactor, with a volume of 150 mL and controlled temperature (20°C). The first set of experiments was carried out with ultrapure water to determine optimal iron and H 2O 2 concentrations. The second was performed with simulated wastewater of pineapple processing. Results: The optimized operational conditions for both iron complexes were 10 mg L -1 of Fe (III) and 25 mg L -1 of H 2O 2, since more than 80% of micropollutants (MP) (at an initial concentration of 1 mg L -1 of each compound) were removed in only 20 min with both DTPA-Fe and EDDS-Fe. The effect of organic matter and inorganic salts on radicals scavenging and chelates stability was also investigated in the experiments performed with synthetic pineapple processing wastewater. The results disclosed differences depending on the iron complex. Nitrites were the principal component influencing the tests carried out with EDDS-Fe. While carbonates at low concentration only significantly affected the experiments performed with DTPA-Fe, they were the major influence on the MPs removal efficiency decrease. In contrast, the presence of Ca 2+ and Mg 2+ only influence on this last one. Finally, the results of phytotoxicity disclosed the suitability of treated effluent to be reused in the agricultural sector.  Conclusions: This work demonstrated that solar powered photo-Fenton catalysed by iron fertilizer EDDS is a suitable technology for depolluting water streams coming from pineapple processing plants at circumneutral pH, and its subsequent reuse for crop irrigation.

RESUMO

ABSTRACT: Composting and vermicomposting before addition to the soil is a viable alternative to the disposal of cattle manure. However, this residue has been used in the untreated form for seedling production. This study evaluated the use of cattle manure in natura, and the organic composted or vermicomposted variants of cattle manure on substrates used in the production of Eucalyptus urograndis seedlings. The treatments consisted of substrates formulated by mixing the organic fertilizers with washed sand in 20, 40, 60, 80 or 100 (v/v) percentages, compared to a commercial substrate. The E. urograndis seedlings were grown in tubes in a greenhouse and evaluated after 120 days for shoot and root dry weights, plant height, stem diameter, shoot/root ratio, and Dickson quality index. The substrate formulated from a mixture of 80% (v/v) of cattle manure in natura and washed sand allows for better development in Eucalyptus seedlings. Substrate containing 100% bovine manure vermicompost or organic compost produced seedlings with lower quality than in natura bovine manure but superior to the commercial substrate. Due to the health risks associated with use of untreated bovine manure, organic compost and vermicompost are good alternatives for the production of E. urograndis seedlings.

RESUMO: Uma alternativa para destinar corretamente o esterco bovino é realizar a compostagem e/ou vermicompostagem e depois adicioná-lo ao solo como fonte de nutrientes às plantas. Porém, este resíduo tem sido utilizado na produção de mudas sem tratamento. O estudo teve por objetivo avaliar o uso de esterco bovino in natura, de composto orgânico e de vermicomposto, produzidos a partir do esterco bovino, em substratos para a produção de mudas de Eucalyptus urograndis. Os tratamentos constituíram-se dos substratos formulados pela mistura dos fertilizantes orgânicos com areia lavada nas porcentagens de 20, 40, 60, 80 e 100 (v/v), em comparação com um substrato comercial (SC). As mudas de Eucalyptus urograndis foram cultivadas em tubetes, em casa de vegetação, e avaliadas aos 120 dias quanto a massa seca da parte aérea e raiz, altura da planta, diâmetro do caule, relação parte aérea/raiz e índice de qualidade de Dickson. O substrato formulado pela mistura de 80% (v/v) de esterco bovino in natura com areia lavada proporcionou o melhor desenvolvimento das mudas de eucalipto. O substrato contendo 100% de vermicomposto de esterco bovino ou composto orgânico produziu mudas com qualidade inferior ao esterco bovino in natura, mas superior ao substrato comercial. Devido aos riscos à saúde associados ao uso de esterco bovino não tratado, o composto orgânico e o vermicomposto são boas alternativas para a produção de mudas de E. urograndis.

RESUMO

Composting and vermicomposting before addition to the soil is a viable alternative to the disposal of cattle manure. However, this residue has been used in the untreated form for seedling production. This study evaluated the use of cattle manure in natura, and the organic composted or vermicomposted variants of cattle manure on substrates used in the production of Eucalyptus urograndis seedlings. The treatments consisted of substrates formulated by mixing the organic fertilizers with washed sand in 20, 40, 60, 80 or 100 (v/v) percentages, compared to a commercial substrate. The E. urograndis seedlings were grown in tubes in a greenhouse and evaluated after 120 days for shoot and root dry weights, plant height, stem diameter, shoot/root ratio, and Dickson quality index. The substrate formulated from a mixture of 80% (v/v) of cattle manure in natura and washed sand allows for better development in Eucalyptus seedlings. Substrate containing 100% bovine manure vermicompost or organic compost produced seedlings with lower quality than in natura bovine manure but superior to the commercial substrate. Due to the health risks associated with use of untreated bovine manure, organic compost and vermicompost are good alternatives for the production of E. urograndis seedlings.

Uma alternativa para destinar corretamente o esterco bovino é realizar a compostagem e/ou vermicompostagem e depois adicioná-lo ao solo como fonte de nutrientes às plantas. Porém, este resíduo tem sido utilizado na produção de mudas sem tratamento. O estudo teve por objetivo avaliar o uso de esterco bovino in natura, de composto orgânico e de vermicomposto, produzidos a partir do esterco bovino, em substratos para a produção de mudas de Eucalyptus urograndis. Os tratamentos constituíram-se dos substratos formulados pela mistura dos fertilizantes orgânicos com areia lavada nas porcentagens de 20, 40, 60, 80 e 100 (v/v), em comparação com um substrato comercial (SC). As mudas de Eucalyptus urograndis foram cultivadas em tubetes, em casa de vegetação, e avaliadas aos 120 dias quanto a massa seca da parte aérea e raiz, altura da planta, diâmetro do caule, relação parte aérea/raiz e índice de qualidade de Dickson. O substrato formulado pela mistura de 80% (v/v) de esterco bovino in natura com areia lavada proporcionou o melhor desenvolvimento das mudas de eucalipto. O substrato contendo 100% de vermicomposto de esterco bovino ou composto orgânico produziu mudas com qualidade inferior ao esterco bovino in natura, mas superior ao substrato comercial. Devido aos riscos à saúde associados ao uso de esterco bovino não tratado, o composto orgânico e o vermicomposto são boas alternativas para a produção de mudas de E. urograndis.

Assuntos

RESUMO

Composting and vermicomposting before addition to the soil is a viable alternative to the disposal of cattle manure. However, this residue has been used in the untreated form for seedling production. This study evaluated the use of cattle manure in natura, and the organic composted or vermicomposted variants of cattle manure on substrates used in the production of Eucalyptus urograndis seedlings. The treatments consisted of substrates formulated by mixing the organic fertilizers with washed sand in 20, 40, 60, 80 or 100 (v/v) percentages, compared to a commercial substrate. The E. urograndis seedlings were grown in tubes in a greenhouse and evaluated after 120 days for shoot and root dry weights, plant height, stem diameter, shoot/root ratio, and Dickson quality index. The substrate formulated from a mixture of 80% (v/v) of cattle manure in natura and washed sand allows for better development in Eucalyptus seedlings. Substrate containing 100% bovine manure vermicompost or organic compost produced seedlings with lower quality than in natura bovine manure but superior to the commercial substrate. Due to the health risks associated with use of untreated bovine manure, organic compost and vermicompost are good alternatives for the production of E. urograndis seedlings.(AU)

Uma alternativa para destinar corretamente o esterco bovino é realizar a compostagem e/ou vermicompostagem e depois adicioná-lo ao solo como fonte de nutrientes às plantas. Porém, este resíduo tem sido utilizado na produção de mudas sem tratamento. O estudo teve por objetivo avaliar o uso de esterco bovino in natura, de composto orgânico e de vermicomposto, produzidos a partir do esterco bovino, em substratos para a produção de mudas de Eucalyptus urograndis. Os tratamentos constituíram-se dos substratos formulados pela mistura dos fertilizantes orgânicos com areia lavada nas porcentagens de 20, 40, 60, 80 e 100 (v/v), em comparação com um substrato comercial (SC). As mudas de Eucalyptus urograndis foram cultivadas em tubetes, em casa de vegetação, e avaliadas aos 120 dias quanto a massa seca da parte aérea e raiz, altura da planta, diâmetro do caule, relação parte aérea/raiz e índice de qualidade de Dickson. O substrato formulado pela mistura de 80% (v/v) de esterco bovino in natura com areia lavada proporcionou o melhor desenvolvimento das mudas de eucalipto. O substrato contendo 100% de vermicomposto de esterco bovino ou composto orgânico produziu mudas com qualidade inferior ao esterco bovino in natura, mas superior ao substrato comercial. Devido aos riscos à saúde associados ao uso de esterco bovino não tratado, o composto orgânico e o vermicomposto são boas alternativas para a produção de mudas de E. urograndis.(AU)

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The thermally dimorphic fungus Histoplasma capsulatum is the causative agent of histoplasmosis, one of the most prevalent endemic mycosis in the Americas. In tropical regions, agro-ecosystems require organic matter replacement, therefore, the use of organic fertilizers has increased disregarding the fact that certain number of such fertilizers might be contaminated with the fungus, and with their handling resulting in human cases and even outbreaks of histoplasmosis. Additionally, in Colombia, chicken manure is the most common raw material used in the production of organic fertilizers. In this work, we reported the isolation of this fungus from chicken manure, and genetically compared with 42 clinical isolates. The genetically compared environmental isolates grouped together with the clinical ones. Our result suggests that chicken manure may be one of H. capsulatum infection sources. Also, the phylogenetic analyses done with other H. capsulatum isolates indicate that the Colombian isolates are widely distributed in the relational tree thus reveling towards the great genetic diversity among the H. capsulatum Colombian isolates.

RESUMO

Acidobacteria is a predominant bacterial phylum in tropical agricultural soils, including sugarcane cultivated soils. The increased need for fertilizers due to the expansion of sugarcane production is a threat to the ability of the soil to maintain its potential for self-regulation in the long term, in witch carbon degradation has essential role. In this study, a culture-independent approach based on high-throughput DNA sequencing and microarray technology was used to perform taxonomic and functional profiling of the Acidobacteria community in a tropical soil under sugarcane (Saccharum spp.) that was supplemented with nitrogen (N) combined with vinasse. These analyses were conducted to identify the subgroup-level responses to chemical changes and the carbon (C) degradation potential of the different Acidobacteria subgroups. Eighteen Acidobacteria subgroups from a total of 26 phylogenetically distinct subgroups were detected based on high-throughput DNA sequencing, and 16 gene families associated with C degradation were quantified using Acidobacteria-derived DNA microarray probes. The subgroups Gp13 and Gp18 presented the most positive correlations with the gene families associated with C degradation, especially those involved in hemicellulose degradation. However, both subgroups presented low abundance in the treatment containing vinasse. In turn, the Gp4 subgroup was the most abundant in the treatment that received vinasse, but did not present positive correlations with the gene families for C degradation analyzed in this study. The metabolic potential for C degradation of the different Acidobacteria subgroups in sugarcane soil amended with N and vinasse can be driven in part through the increase in soil nutrient availability, especially calcium (Ca), magnesium (Mg), potassium (K), aluminum (Al), boron (B) and zinc (Zn). This soil management practice reduces the abundance of Acidobacteria subgroups, including those potentially involved with C degradation in this agricultural soil.

RESUMO

A ciclagem de resíduos de origem animal pelo processo de compostagem e sua transformação em insumos para produção vegetal além de minimizarem os impactos ambientais, podem ser uma importante fonte de renda ao produtor. Além disso, a compostagem desses resíduos produz um insumo agrícola com elevado potencial na produção de hortaliças e com possibilidade de associação com organismos de controle biológico, possibilitando a produção de um substrato ecologicamente correto, rico em nutrientes e protetor da saúde das plantas. Nesse sentido, o objetivo deste trabalho foi avaliar a sobrevivência do fungo Trichoderma spp. durante o processo de vermicompostagem, as alterações físico/químicas ocasionadas no substrato e o crescimento e desenvolvimento de plantas de alface cultivadas. Foram avaliados, como substratos base, resíduos da criação de ovinos; cavalos e coelhos, com ou sem a adição de Trichoderma spp. Em todos os tratamentos foram adicionadas minhocas da espécie Eisenia fetida. Os tratamentos foram arranjados em esquema fatorial 3x2 e o delineamento experimental foi o inteiramente casualizado, com 4 repetições O fungo Trichoderma spp. sobrevive durante o processo de vermicompostagem dos diferentes resíduos. A utilização de Trichoderma spp. não teve efeito nos teores de fósforo, magnésio e matéria orgânica, reduziu os valores de cálcio, elevou os teores de potássio para o substrato a base de coelho, aumentou a densidade seca do substrato base de resíduos de coelho e reduziu do substrato base de resíduos de cavalos. O substrato que proporcionou o melhor crescimento e desenvolvimento de plantas de alface foi o de resíduos de criação de cavalos, sendo favorecido pela presença de Trichoderma spp. no segundo ano de cultivo.(AU)

The intensification of agropastoral activity leads to a high production of waste. The composting of these residues produces an agricultural input with high potential in the production of vegetables and with the possibility of association with organisms of biological control, allowing the production of an ecologically correct substrate, rich in nutrients and protector of the health of the plants. In this sense, the objective of this work was to evaluate the survival of the fungus Trichoderma spp. During the vermicompost process,as the physical / chemical development caused not substrate and the growth and development of lettuce plants grown in the different treatments used. Basal substrates were obtained from the creation of three different animals: sheep, Horses and rabbits, whether or not containing added Trichoderma spp. In all treatments, worms of Eisenia foetida were added. Evaluations of fungus survival, physical and chemical analyzes of the substrate produced and evaluation without growth and development of lettuce plants were carried out. The treatments were arranged in a 3x2 factorial scheme and the experimental design was completely randomized, with 4 replicates. Data were analyzed using Sisvar® software, and as means averages compared by Scott Knott's test at 5% error probability. Trichoderma spp. It survives the vermicompost process by improving the development of lettuce plants when associated with farmsresidue. The use of Trichoderma spp. It reduced the calcium values of all substrates, raised a dry density of the base substrate of rabbit residues, and reduced the base substrate of farmyard residues. The substrate that provided the best growth and development of lettuce plants was the residues of rearing of horses, being favored by the presence of Trichoderma spp. in the second year.(AU)

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RESUMO

A ciclagem de resíduos de origem animal pelo processo de compostagem e sua transformação em insumos para produção vegetal além de minimizarem os impactos ambientais, podem ser uma importante fonte de renda ao produtor. Além disso, a compostagem desses resíduos produz um insumo agrícola com elevado potencial na produção de hortaliças e com possibilidade de associação com organismos de controle biológico, possibilitando a produção de um substrato ecologicamente correto, rico em nutrientes e protetor da saúde das plantas. Nesse sentido, o objetivo deste trabalho foi avaliar a sobrevivência do fungo Trichoderma spp. durante o processo de vermicompostagem, as alterações físico/químicas ocasionadas no substrato e o crescimento e desenvolvimento de plantas de alface cultivadas. Foram avaliados, como substratos base, resíduos da criação de ovinos; cavalos e coelhos, com ou sem a adição de Trichoderma spp. Em todos os tratamentos foram adicionadas minhocas da espécie Eisenia fetida. Os tratamentos foram arranjados em esquema fatorial 3x2 e o delineamento experimental foi o inteiramente casualizado, com 4 repetições O fungo Trichoderma spp. sobrevive durante o processo de vermicompostagem dos diferentes resíduos. A utilização de Trichoderma spp. não teve efeito nos teores de fósforo, magnésio e matéria orgânica, reduziu os valores de cálcio, elevou os teores de potássio para o substrato a base de coelho, aumentou a densidade seca do substrato base de resíduos de coelho e reduziu do substrato base de resíduos de cavalos. O substrato que proporcionou o melhor crescimento e desenvolvimento de plantas de alface foi o de resíduos de criação de cavalos, sendo favorecido pela presença de Trichoderma spp. no segundo ano de cultivo.

The intensification of agropastoral activity leads to a high production of waste. The composting of these residues produces an agricultural input with high potential in the production of vegetables and with the possibility of association with organisms of biological control, allowing the production of an ecologically correct substrate, rich in nutrients and protector of the health of the plants. In this sense, the objective of this work was to evaluate the survival of the fungus Trichoderma spp. During the vermicompost process,as the physical / chemical development caused not substrate and the growth and development of lettuce plants grown in the different treatments used. Basal substrates were obtained from the creation of three different animals: sheep, Horses and rabbits, whether or not containing added Trichoderma spp. In all treatments, worms of Eisenia foetida were added. Evaluations of fungus survival, physical and chemical analyzes of the substrate produced and evaluation without growth and development of lettuce plants were carried out. The treatments were arranged in a 3x2 factorial scheme and the experimental design was completely randomized, with 4 replicates. Data were analyzed using Sisvar® software, and as means averages compared by Scott Knott's test at 5% error probability. Trichoderma spp. It survives the vermicompost process by improving the development of lettuce plants when associated with farmsresidue. The use of Trichoderma spp. It reduced the calcium values of all substrates, raised a dry density of the base substrate of rabbit residues, and reduced the base substrate of farmyard residues. The substrate that provided the best growth and development of lettuce plants was the residues of rearing of horses, being favored by the presence of Trichoderma spp. in the second year.

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RESUMO

Resumen En suelos del Valle del Sinú Medio de mediana fertilidad se evaluó, en un cultivo de maíz hibrido, el efecto de varias fuentes de abonos orgánicos, cuyos tratamientos fueron los siguientes: T1 (Bocashi), T2 (compost con volteos semanales sin microorganismos eficientes (EM), T3 (compost con volteos semanales con EM), T4 (compost con volteos quincenales sin EM), T5 (compost con volteos quincenales con EM), TR (testigo relativo, con NPK en dosis de 100-40-30 kg.ha-1), y T0 (testigo absoluto, sin aplicación de abono ni fertilizante). La dosis de abono utilizado fue de 2 t.ha-1. Las variables evaluadas fueron rendimiento de grano y crecimiento del cultivo, de las cuales se estimaron los índices fisiológicos (TCA o tasa de crecimiento absoluto, TCR o tasa de crecimiento relativo y TAN o tasa de asimilación neta); el diseño experimental usado fue de bloques completos al azar. Se obtuvo que los mayores rendimientos de grano correspondieron a la fertilización química (6.86 t. ha-1) y a la fertilización con Bocashi (6.40 t. ha-1) sin diferencias estadísticas significativas entre estos dos tratamientos. Por otro lado, no se encontraron diferencias estadísticas significativas en los parámetros fisiológicos evaluados como altura de planta, número de hojas, masa seca y área foliar. Para este experimento se concluye que el Bocashi puede sustituir la fertilización química bajo las condiciones edafoclimáticas del Valle del Sinú Medio

Abstrac In soils of Valle de Sinú Medio of medium fertility, the effect of several sources of organic fertilizers was evaluated in a hybrid corn crop, whose treatments were the following:: T1 (Bocashi), T2 (compost with weekly mixture without effective microorganisms EM-); T3(compost weekly mixture with EM); T4 (compost with fortnightly mixture without MS); T5 (compost with fortnightly mixture with MS), T6 at doses 2 t.ha-1 for all treatments with organic fertilizers and the absolute check (T0) received no fertilizers, while the relative check (TR) was fertilized with NPK doses of 100-40-30 kg.ha-1. The evaluated variables were grain yield and crop growth, of which the physiological indices were estimated (TCA or absolute growth rate, TCR or relative growth rate and TAN or net assimilation rate); the experimental design used was randomized complete blocks. It was found that the highest grain yield corresponded to chemical fertilization (6.86 t ha-1) and fertilization with Bocashi (6.40 t ha-1) without significant statistical differences between these two treatments. On the other hand, no significant statistical differences were found in the physiological parameters evaluated as plant height, number of leaves, dry mass and foliar area. For this experiment it is concluded that the Bocashi can substitute the chemical fertilization under the edaphoclimatic conditions of the Valley of the Middle Sinú.

Resumo Em solos de Valle del Sinu Medio de fertilidade média, foi availado o efeito de diversas fontes de fertilizantes orgânicos em uma cultura de milho híbrido, cujos tratamentos foram os seguintes: T1 (Bocashi), T2 (compost semanal misturando sem microrganismos eficazes EM), T3 (compost semanal misturando mais MS), T4 (compost quinsenal misturado sem MS), T5 (compost quinzenal misturado com MS), em doses 2 t ha-1 para todas as tratamentos e tratamento control (T0) sem nenhum fertilizante ou adubos orgânicos, enquanto a tratamento control relativo (TR) só fertilizado com NPK en doses 100-40-30 kg ha-1. As variáveis avaliadas foram rendimento de grãos e crescimento da cultura da milho, das quais foram estimados os índices fisiológicos (TCA ou taxa de crescimento absoluto, TCR ou taxa de crescimento relativo e TAN ou taxa de assimilação líquida), o delineamento experimental utilizado foi em blocos inteiramente casualizado. Verificou-se que os maiores rendimentos de grãos corresponderam à adubação química (6,86 t ha-1) e a adubação com Bocashi (6,40 t ha-1), sem diferenças estatisticamente significativas entre os dois tratamentos. Por outro lado, não foram encontradas dife renças estatisticamente significantes nos parâmetros fisiológicos avaliados como altura de planta, número de folhas, massa seca e área foliar. Para este experimento conclui-se que o Bocashi pode substituir a adubação química sob as condições edafoclimáticas do Vale do Médio Sinú.

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Among the lighter elements having two or more stable isotopes (H, C, N, O, S), δ(15)N appears to be the most promising isotopic marker to differentiate plant products from conventional and organic farms. Organic plant products vary within a range of δ(15)N values of +0.3 to +14.6%, while conventional plant products range from negative to positive values, i.e. -4.0 to +8.7%. The main factors affecting δ(15)N signatures of plants are N fertilizers, biological N2 fixation, plant organs and plant age. Correlations between mode of production and δ(13)C (except greenhouse tomatoes warmed with natural gas) or δ(34)S signatures have not been established, and δ(2)H and δ(18)O are unsuitable markers due to the overriding effect of climate on the isotopic composition of plant-available water. Because there is potential overlap between the δ(15)N signatures of organic and conventionally produced plant products, δ(15)N has seldom been used successfully as the sole criterion for differentiation, but when combined with complementary analytical techniques and appropriate statistical tools, the probability of a correct identification increases. The use of organic fertilizers by conventional farmers or the marketing of organic produce as conventional due to market pressures are additional factors confounding correct identification. The robustness of using δ(15)N to differentiate mode of production will depend on the establishment of databases that have been verified for individual plant products.

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RESUMO A espécie Arrabidaea chica pertencente à família Bignoniaceae é também conhecida popularmente como crajiru. É Utilizada tradicionalmente no tratamento de enfermidades da pele, inflamações, infecções, dentre outros. O objetivo deste trabalho foi avaliar a produção de biomassa aérea, teor e rendimento de extrato de crajiru em função de diferentes fontes de adubo orgânico em Manaus, AM. As mudas foram formadas através de estacas e mantidas por 60 dias em casa de vegetação. O experimento foi conduzido em esquema fatorial 5x3 com delineamento em blocos casualizados onde os níveis dos fatores foram as diferentes fontes de adubo orgânico: composto (5,0 kg/m2), esterco de aves (3,0 kg/m2), casca de guaraná (4,0 kg/m2), esterco de gado (4,0 kg/m2) e controle (ausência), e os três morfotipos de crajiru. Obedeceu-se o espaçamento de 1,0x1,0 m entre planta e 2,0 m entre blocos. Após 240 dias foram avaliadas a produção de folhas e caules, relação folha/caule, teor de extratos hexânico concentrado em evaporador rotativo obtido pela fórmula: (massa do extrato/massa da amostra total)x100; e o rendimento de extrato estimado pela equação: (%extrato x massa seca total das folhas)/100. Os dados foram submetidos à análise de variância e as médias ao Teste de Tukey (p<0,05). As fontes de adubo orgânico promoveram melhor desenvolvimento das plantas de, exceto para o morfotipo 1. O esterco de aves proporcionou maior produção de biomassa, teor e rendimento de extratos. Dessa forma, sugere-se que a adubação orgânica propicia maior produção de biomassa, teor e rendimento de extratos de A. chica.

ABSTRACT The Arrabidaea chicaspecies belongs to the Bignoniaceae family and is also popularly known as crajiru. It is traditionally used to treat skin diseases, inflammations and infections. The objective of this study was to evaluate the biomass production, the content and the yield of crajiru extract in face of different sources of organic fertilizer in Manaus, AM. The seedlings were formed by cuttings and kept for 60 days in a greenhouse. The trial was conducted in a 5x3 factorial design with randomized block organization where the factor levels were different sources of organic fertilizer: compound (5.0 kg / m 2), chicken manure (3.0 kg / m 2), bark guarana (4.0 kg / m2), cattle manure (4.0 kg / m 2) and control (absence), and the three morphotypes of crajiru. It was obeyed the spacing between the plants (1,0 x1,0m) and the blocks (2,0m) . After 240 days it were evaluated the production of leaves and stems, the leaf / stem ratio, and the content of hexane extracts concentrated by rotary evaporation obtained by the following formula: (mass of extract / mass of total sample) x100; and the yield estimated by the equation extract: (% extract x total dry mass of leaves) / 100. The data were subjected to analysis of variance and means to the Tukey"s test (p <0.05). The sources of organic fertilizer promoted better development of plants, except for the morphotype 1. The poultry manure provided higher biomass, content and yield of extracts. Thus, it is suggested that the organic fertilizer provides greater biomass production, content and yield of A. chica extracts.

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A utilização de biofertilizante na agricultura é uma alternativa promissora aos fertilizantes minerais e de destino de dejetos. Objetivou-se neste estudo avaliar características morfológicas, produção de biomassa e teores de nutrientes foliares do milho e do milheto irrigados e adubados com biofertilizante suíno. Foram conduzidos dois experimentos, com milho e com milheto no período de junho a setembro de 2011 em um latossolo vermelho amarelo, em delineamento de blocos casualizados, com seis tratamentos e quatro repetições, a saber: doses de biofertilizante suíno para fornecer 0, 50, 100, 150 e 200 kg/ha de P2O5, e um tratamento adicional com adubo mineral NPK 4-14-8. Cada uma das doses de fósforo fornecida pelo biofertilizante suíno foi comparada, pelo teste de Dunett, ao tratamento adicional com adubo mineral, e a significância foi declarada quando P<0,05. Para as doses de fósforo fornecidas via biofertilizante suíno foram ajustadas equações de regressão. As plantas de milho e milheto responderam de forma linear, quanto à produção de biomassa, com o aumento das doses de biofertilizante suíno. Na dose de biofertilizante suíno que proporcionou a aplicação de 150 kg/ha de P2O5, a produção de biomassa para silagem e os teores foliares de nutrientes, tanto do milho quanto do milheto, foram semelhantes aos obtidos com a adubação mineral...

The use of biofertilizers in agriculture is a promising alternative to mineral fertilizers and as a destination of manure. The objective of this study was to evaluate the morphological characteristics, biomass production and leaf nutrient content of irrigated corn and millet fertilized with swine biofertilizer. Two experiments were conducted between June and September 2011 using corn and millet grown on a Red-Yellow Latosol in a randomized block design consisting of six treatments and four repetitions: swine biofertilizer doses to provide 0, 50, 100, 150 and 200 kg/ha P2O5 and one additional treatment using NPK 4-14-8 mineral fertilizer. Each dose of phosphorus provided by the swine biofertilizer was compared by the Dunnett test to the additional treatment with mineral fertilizer and significance was defined when P<0.05. Regression equations were fitted for the phosphorus doses provided by the swine biofertilizer. The biomass production of the corn and millet plants responded linearly to increasing doses of the swine biofertilizer. At the dose that provided the application of 150 kg/ha P2O5, silage production and leaf nutrient content of corn and millet were similar to those obtained with the mineral fertilizer...

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A utilização de biofertilizante na agricultura é uma alternativa promissora aos fertilizantes minerais e de destino de dejetos. Objetivou-se neste estudo avaliar características morfológicas, produção de biomassa e teores de nutrientes foliares do milho e do milheto irrigados e adubados com biofertilizante suíno. Foram conduzidos dois experimentos, com milho e com milheto no período de junho a setembro de 2011 em um latossolo vermelho amarelo, em delineamento de blocos casualizados, com seis tratamentos e quatro repetições, a saber: doses de biofertilizante suíno para fornecer 0, 50, 100, 150 e 200 kg/ha de P2O5, e um tratamento adicional com adubo mineral NPK 4-14-8. Cada uma das doses de fósforo fornecida pelo biofertilizante suíno foi comparada, pelo teste de Dunett, ao tratamento adicional com adubo mineral, e a significância foi declarada quando P<0,05. Para as doses de fósforo fornecidas via biofertilizante suíno foram ajustadas equações de regressão. As plantas de milho e milheto responderam de forma linear, quanto à produção de biomassa, com o aumento das doses de biofertilizante suíno. Na dose de biofertilizante suíno que proporcionou a aplicação de 150 kg/ha de P2O5, a produção de biomassa para silagem e os teores foliares de nutrientes, tanto do milho quanto do milheto, foram semelhantes aos obtidos com a adubação mineral...(AU)

The use of biofertilizers in agriculture is a promising alternative to mineral fertilizers and as a destination of manure. The objective of this study was to evaluate the morphological characteristics, biomass production and leaf nutrient content of irrigated corn and millet fertilized with swine biofertilizer. Two experiments were conducted between June and September 2011 using corn and millet grown on a Red-Yellow Latosol in a randomized block design consisting of six treatments and four repetitions: swine biofertilizer doses to provide 0, 50, 100, 150 and 200 kg/ha P2O5 and one additional treatment using NPK 4-14-8 mineral fertilizer. Each dose of phosphorus provided by the swine biofertilizer was compared by the Dunnett test to the additional treatment with mineral fertilizer and significance was defined when P<0.05. Regression equations were fitted for the phosphorus doses provided by the swine biofertilizer. The biomass production of the corn and millet plants responded linearly to increasing doses of the swine biofertilizer. At the dose that provided the application of 150 kg/ha P2O5, silage production and leaf nutrient content of corn and millet were similar to those obtained with the mineral fertilizer...(AU)

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A fast and accurate method based on ultrasound-assisted extraction in a cup-horn sonoreactor was developed to determine the total content of Cd, Co, Cr, Cu, Mn, Ni, Pb and Zn in organic fertilizers by fast sequential flame atomic absorption spectrometry (FS FAAS). Multivariate optimization was used to establish the optimal conditions for the extraction procedure. An aliquot containing approximately 120 mg of the sample was added to a 500 µL volume of an acid mixture (HNO3/HCl/HF, 5:3:3, v/v/v). After a few minutes, 500 µL of deionized water was added and eight samples were simultaneously sonicated for 10 min at 50% amplitude, allowing a sample throughput of 32 extractions per hour. The performance of the method was evaluated with a certified reference material of sewage sludge (CRM 029). The precision, expressed as the relative standard deviation, ranged from 0.58% to 5.6%. The recoveries of analytes were found to 100%, 109%, 96%, 92%, 101%, 104% and 102% for Cd, Cr, Cu, Mn, Ni, Pb and Zn, respectively. The linearity, limit of detection and limit of quantification were calculated and the values obtained were adequate for the quality control of organic fertilizers. The method was applied to the analysis of several commercial organic fertilizers and organic wastes used as fertilizers, and the results were compared with those obtained using the microwave digestion procedure. A good agreement was found between the results obtained by microwave and ultrasound procedures with recoveries ranging from 80.4% to 117%. Two organic waste samples were not in accordance with the Brazilian legislation regarding the acceptable levels of contaminants.

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Objetivo: Demostrar la presencia de Rotavirus en las diferentes fases de un proceso de compostaje: matrices utilizadas como materia prima, mezcla a compostar y producto terminado. Materiales y métodos. El Rotavirus se determinó durante los tres procesos de compostaje. La detección viral se realizó por inmunocromatografía, ELISA y RT- PCR. Resultados. Se evidenció presencia viral en el primer proceso de compostaje, ausencia viral en el segundo y en el tercer proceso de compostaje, se presentaron interferencias que dificultaron interpretar los resultados de la PCR, lo cual impidió llegar a un resultado concluyente de su presencia en el abono. Conclusiones. Los abonos orgánicos pueden ser portadores de virus motivo por el cual se deben implementar pruebas de calidad para evitar que este material contribuya con la diseminación viral. Dentro de estos abonos existen sustancias capaces de interferir en las pruebas de detección.

Objective. To show the presence of rotavirus in different stages of a composting process: matrices used as raw material, mixture to be composted and the final product. Materials and methods. Immunochromatography, ELISA and RT-PCR were used for viral detection. Results. Rotavirus was found in the first composting step, no virus was found in the second step, and some inhibitory substances were found in the third step that posed difficulties in interpreting the PCR results and therefore providing a concluding result on rotavirus presence in the final product. Conclusions. Organic fertilizers can be vectors of human pathogenic viruses; therefore quality control tests must be implemented to avoid further viral dissemination. There are inhibitory substances present in organic fertilizers capable of interfering with the detection tests.

Objetivo. Demonstrar a presença de Rotavírus nas diferentes fases de um processo de compostagem: matrizes utilizadas como matéria-prima, mistura de composto e produto final. Materiais e métodos: A determinação do Rotavírus foi realizada nos três processos de compostagem. A detecção viral foi realizada por imunocromatografia, ELISA e RT-PCR. Resultados. Evidenciou-se a presença viral no primeiro processo de compostagem, ausência de vírus no segundo, e no terceiro processo de compostagem, apresentaram-se interferências que dificultaram a interpre tação dos resultados da PCR, tornando impossível chegar a um resultado conclusivo de sua presença no adubo. Conclusões. Os adubos orgânicos podem abrigar o vírus é por isso que se devem implementar provas de qualidade para evitar que esse material contribuía com a disseminação viral. Dentro desses fertilizantes existem substâncias capazes de interferir nas provas de detecção.