RESUMO
Herbicide-resistant oilseed rape (Brassica napus) cultivation in our country entails the risk of gene transfer to related wild species. One of these species is the wild turnip (B. rapa), an important weed of winter crops widely distributed in the Pampas region. Despite hybridization risks, Clearfield ® oilseed rape is available in Argentina. In 2008, a B. rapa population, which was sympatric to an imidazolinone-resistant and a conventional oilseed rape cultivar, was located on a farm in the main cropping area of the country. Herbicide-resistant individuals were found in the progeny of this population in a herbicide screening test. Therefore, a molecular characterization using cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR) markers was conducted on these plants to determine their hybrid nature and to establish the origin of the imidazolinone resistance trait. The results of this study, along with information of field records, confirmed that the resistant plants were first generation interspecific hybrids. Imidazolinone resistance had been effectively transferred from the herbicide-resistant oilseed rape, even in the particular situation of pollen competition. Oilseed rape resistant cultivars are becoming more common in the country. So, considering that seed loss and crop volunteers are common in these species, it is crucial to avoid the dispersion of new resistant weed biotypes as they reduce the effectiveness of chemical control technologies.
Assuntos
Brassica napus , Brassica rapa , Argentina , Brassica napus/genética , Brassica rapa/genética , Monitoramento Ambiental , Humanos , Plantas Geneticamente ModificadasRESUMO
Wild turnip (Brassica rapa) is a common weed and a close relative to oilseed rape (Brassica napus). The Clearfield® production system is a highly adopted tool which provides an alternative solution for weed management, but its efficiency is threatened by gene transfer from crop to weed relatives. Crop-weed hybrids with herbicide resistance were found in the progeny of a B. rapa population gathered from a weedy stand on the borders of an oilseed rape (B. napus) imidazolinone (IMI)-resistant crop. Interspecific hybrids were confirmed by morphological traits in the greenhouse and experimental field, survival after imazethapyr applications, DNA content through flow cytometry, and pollen viability. The transference of herbicide resistance was demonstrated even in a particular situation of pollen competition between both an herbicide-resistant crop and a non-resistant crop. However, IMI resistance was not found in further generations collected at the same location. These results verify gene transmission from oilseed rape to B. rapa in the main crop area in Argentina where resistant and susceptible varieties are found and seed loss and crop volunteers are common. Hybridization, introgression, and herbicide selection would be associated with the loss of effectiveness of IMI technology.