RESUMO

ABSTRACT: The present study evaluated the efficiency of a protocol for micropropagation of stem apexes and nodal segments of basil 'Grecco a Palla' in various concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA). A completely randomized design was used with six treatments distributed in five replications. A medium without growth regulators favored the survival of Ocimumbasilicum stem apexes inoculated in vitro, and thereby promoted the sprouting of explants, whereas, for nodal segments, it was necessary to use regulators, and the concentration of 0.5 mg.L−1 BAP 0.0 mg.L−1 of IBA was more beneficial for the species.

RESUMO: O objetivo deste trabalho foi avaliar a eficiência de um protocolo de micropropagação de ápices caulinares e segmentos nodais da cultivar de manjericão (Ocimum basilicum L.) 'Grecco a palla' em diferentes concentrações de BAP (6-benzilaminopurina) e de AIB (ácido 3-indol butírico). Foi utilizado delineamento experimental inteiramente casualizado, com seis tratamentos distribuídos em cinco repetições. Para ápices caulinares, meio sem a adição de reguladores de crescimento favoreceu a sobrevivência de ápices caulinares de O. basilicum inoculados in vitro, promovendo a brotação dos explantes. Enquanto que para segmentos nodais houve necessidade do uso de reguladores, sendo que a concentração de 0,5 mg.L-1 de BAP e 0,0 mg.L-1 de AIB foi mais benéfica para a espécie.

RESUMO

ABSTRACT Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich's experimental tumor (EET) in mice. Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax. Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract. Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.

RESUMO

Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box-Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.

Assuntos

RESUMO

Apergillus carbonarius and Aspergillus niger are the principal fungi that attack table grapes, and they are responsible for producing and contaminating these fruits with ochratoxin A. Packaging containing essential oils from Ocimum gratissimum L. and Ocimum basilicum L. encapsulated in poly(lactic acid) (PLA) nanofibers were produced, the antifungal and antiocratoxigenic activities against A. carbonarius and A. niger were evaluated in vitro and in vivo, and the effect of these packages on the quality of table grapes was determined. The nanofibers were produced by the Solution Blow Spinning technique and characterized by Scanning Electron Microscopy and Thermogravimetric Analysis. Fungal contamination and ochratoxin A production were significantly controlled by PLA nanofibers containing the essential oils and the physicochemical parameters of the grapes were preserved, preserving the quality and the shelf life of the fruit. Therefore, the active packaging developed herein has potential and may be suitable for application in fruits.

Assuntos

RESUMO

ABSTRACT: Tissue culture is an important technique for assessing the influence of light on plant growth. This study evaluated the in vitro cultivation of Ocimum basilicum cultivars under different light sources. First, an experiment was conducted to evaluate the effect of fluorescent and LED (Light Emitting Diode) light sources (yellow, blue, green, and red) on five basil cultivars (Cinnamon, Grecco a Palla, Italian Large, Limoncino, and Maria Bonita), followed by, another experiment, with two LED lights (Growlux and Blue). In vitro basil plants did not exhibit good growth under yellow lamps. The size of the roots of Cinnamon, Grecco a Palla and Limoncino cultivars under the two light conditions were similar. Moreover, the luminous condition of the Growlux lamps generated a greater quantity of leaves and fresh, and dry weight than blue LED lamps for Cinnamon, Grecco a Palla and Limoncino cultivars. Thus, it was concluded that the Growlux lamps promoted a greater amount of basil biomass.

RESUMO: A cultura de tecidos é uma técnica importante para avaliar a influência da luz no desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o cultivo in vitro de cultivares de Ocimum basilicum sob diferentes fontes de luz. Primeiramente, foi avaliado o efeito de fontes de luz fluorescentes e LEDs (Diodo emissores de luz amarela, azul, verde e vermelha) em cinco cultivares de manjericão (Cinnamon, Grecco a Palla, Italian Large, Limoncino e Maria Bonita); e posteriormente o efeito de duas lâmpadas LED (Growlux e Azul). As lâmpadas amarelas não proporcionaram um bom desenvolvimento das plantas de manjericão in vitro. Verificou-se que o tamanho das raízes e massa fresca nas cultivares Cinnamon, Grecco a Palla e Limoncino nas duas condições luminosas foram semelhantes. A condição luminosa das lâmpadas Growlux promoveu maior número de folhas, massa fresca e seca quando comparada com lâmpadas LED azuis nas cultivares Cinnamon, Grecco a Palla e Limoncino. Assim, concluiu-se que as lâmpadas Growlux promovem uma maior quantidade de biomassa de manjericão.

RESUMO

Tissue culture is an important technique for assessing the influence of light on plant growth. This study evaluated the in vitro cultivation of Ocimum basilicum cultivars under different light sources. First, an experiment was conducted to evaluate the effect of fluorescent and LED (Light Emitting Diode) light sources (yellow, blue, green, and red) on five basil cultivars (Cinnamon, Grecco a Palla, Italian Large, Limoncino, and Maria Bonita), followed by, another experiment, with two LED lights (Growlux and Blue). In vitro basil plants did not exhibit good growth under yellow lamps. The size of the roots of Cinnamon, Grecco a Palla and Limoncino cultivars under the two light conditions were similar. Moreover, the luminous condition of the Growlux lamps generated a greater quantity of leaves and fresh, and dry weight than blue LED lamps for Cinnamon, Grecco a Palla and Limoncino cultivars. Thus, it was concluded that the Growlux lamps promoted a greater amount of basil biomass.

A cultura de tecidos é uma técnica importante para avaliar a influência da luz no desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o cultivo in vitro de cultivares de Ocimum basilicum sob diferentes fontes de luz. Primeiramente, foi avaliado o efeito de fontes de luz fluorescentes e LEDs (Diodo emissores de luz amarela, azul, verde e vermelha) em cinco cultivares de manjericão (Cinnamon, Grecco a Palla, Italian Large, Limoncino e Maria Bonita); e posteriormente o efeito de duas lâmpadas LED (Growlux e Azul). As lâmpadas amarelas não proporcionaram um bom desenvolvimento das plantas de manjericão in vitro. Verificou-se que o tamanho das raízes e massa fresca nas cultivares Cinnamon, Grecco a Palla e Limoncino nas duas condições luminosas foram semelhantes. A condição luminosa das lâmpadas Growlux promoveu maior número de folhas, massa fresca e seca quando comparada com lâmpadas LED azuis nas cultivares Cinnamon, Grecco a Palla e Limoncino. Assim, concluiu-se que as lâmpadas Growlux promovem uma maior quantidade de biomassa de manjericão.

Assuntos

RESUMO

Las plantas medicinales se han utilizado tradicionalmente en la medicina popular debido a sus reconocidos efectos curativos naturales. Se estima que alrededor de dos tercios de la población mundial utilizan la medicina tradicional para sus necesidades médicas primarias. La albahaca (Ocimum basilicum L.) es uno de los principales cultivos herbáceos del mundo que ha mostrado componentes que pueden ser beneficiosos para el tratamiento de enfermedades cardiovasculares, trastornos inflamatorios y disminución del riesgo de cáncer. Este artículo presenta una revisión acerca de la planta y semillas de albahaca desde el 2010 a la fecha, con el objetivo de identificar la composición química (macronutrientes-proteínas, lípidos, carbohidratos; compuestos volátiles y polifenoles) y sus beneficios para la salud en función de la evidencia en humanos, modelo in vivo e in vitro. La literatura reciente muestra que las hojas y semillas de albahaca son una buena fuente de ácidos grasos α-linolénico, aceite y polifenoles con propiedades antioxidantes y antiinflamatorias que afectaría de forma favorable en la salud, restaurando la homeostasis en diversas patologías. Sin embargo, hasta la fecha, no se han dilucidado por completo los mecanismos moleculares involucrados.

Medicinal plants have traditionally been used in folk medicine for their natural healing effects. It is estimated that around two-thirds of the world's population uses traditional medicine for their primary medical needs. Basil (Ocimum basilicum) is one of the main herbal crops in the world that has shown components that can be beneficial for the treatment of cardiovascular diseases, inflammatory disorders, and decreased risk of cancer. This article presents a review of the state of the art about the basil plant and seeds from 2010 to date, with the aim of identifying the chemical composition (macronutrients-proteins, lipids, carbohydrates; volatile compounds and polyphenols) and its benefits on health based on evidence in humans, in vivo and in vitro models. Recent literature shows that basil leaves and seeds are a good source of α-linolenic fatty acids, essential oils and polyphenols with antioxidant and anti-inflammatory properties that would have a favorable impact on health, restoring homeostasis in various pathologies. However, to date, the molecular mechanisms involved have not been fully elucidated.

RESUMO

Poly(lactic acid) (PLA) nanofibres containing different proportions of the essential oils from Ocimum basilicum L. and Ocimum gratissimum L. were prepared by solution blow spinning method. The essential oils were extracted by hydrodistillation and characterized by gas chromatography. MEV, contact angle, DSC and FTIR were used to characterize the nanofibres. The effect of bioative nanofibres on the growth of the fungus and on the production of ochratoxin A were evaluated using the fumigation test. Linalool, 1·8-cineole and camphor were the principal components of the essential oil from O. basilicum, and eugenol was the principal constituent in the oil from O. gratissimum. An increase in the average diameter of the nanofibres was observed with the addition of the essential oils. The essential oils acted as a plasticizer, resulting in a reduction in the crystallinity of the PLA. The encapsulation of essential oils in PLA nanofibres was verified by FTIR. An effective antifungal and antimicotoxygenic activity against Aspergillus ochraceus and Aspergillus westerdjikiae was observed for the bioative nanofibres. These results confirm the potential of PLA nanofibres containing the essential oils for the control of toxigenic fungi that cause the deterioration of food and are harmful to human health.

Assuntos

RESUMO

Considering the growing water crisis, using wastewater helps reduce the pressure on good-quality water consumption. In this context, this study aimed to evaluate the growth, production, and essential oil content of Alfavaca Basilicão and Grecco a Palla basil genotypes with a DFT (deep flow technique) hydroponic system adapted to PVC tubes at different recirculation intervals of nutrient solutions prepared with treated domestic effluents. The experiment was performed in a greenhouse with Alfavaca Basilicão and Grecco a Palla basil genotypes analyzed individually. It was a completely randomized design with four replicates in a 2 x 3 factorial scheme: two types of water (public-supply water and wastewater) and three recirculation intervals of nutrient solutions (2, 4, and 6 hours). At 35 days after transplanting, the study evaluated plant height, stem diameter, shoot fresh mass, shoot dry mass, water consumption, water use efficiency, and essential oil content and yield. The types of water did not significantly influence the variables of growth, production, and essential oil content of the basil genotypes. Basil cultivation with the DFT system in tubes is technically feasible with nutrient solutions prepared with treated domestic effluents and recirculation every 2 and 6 hours for Grecco a Palla and Alfavaca Basilicão genotypes, respectively. The means of essential oil content were 1.6 and 1.7% (v/m) for Alfavaca Basilicão and Grecco a Palla genotypes, respectively.

Assuntos

RESUMO

Basil (Ocimum basilicumL.) is a medicinal species used in several areas, such as food, medicines and cosmetics, and the understanding of its physiological behavior under environmental conditions is of paramount importance for the improvement of cultivation methods. The objective of this study was to evaluate the influence of different water availability under physiological, biochemical and metabolic characteristics, in three distinct genotypes: 'Alfavaca basilicão', 'Gennaro de menta' and 'Grecco à palla', during two different phenological stages (vegetative and reproductive). It was found that the water deficit promotes physiological changes to tolerate water stress, and the studied genotypes have different routes to achieve this physiological tolerance, which culminates in a distinct accumulation of metabolites in plants, and can be considered interesting if the final product is the production of essential oils.

La albahaca (Ocimum basilicum L.) es una planta medicinal utilizada en varias áreas: alimenticia, medicinal e industria cosmética; es de suma importancia el entendimiento de su comportamiento fisiológico bajo diferentes condiciones ambientales con el fin de mejorar los procesos del cultivo. El objetivo de este estudio fue evaluar la influencia de diferentes disponibilidades hídricas en las características fisiológicas, bioquímicas y metabólicas en tres genotipos de albahaca: "Alfavaca basilicão", "Gennaro de menta" y "Grecco à palla" durante dos etapas fenológicas (vegetativa y reproductiva). Fue encontrado que el déficit hídrico promueve cambios fisiológicos con el fin de tolerar el estrés hídrico. Los genotipos estudiados presentaron diferentes rutas para alcanzar esta tolerancia fisiológica, la cual culmina con distintas acumulaciones de metabolitos en las plantas, y puede ser considerado interesante si el producto final es la producción de aceites esenciales.

Assuntos

RESUMO

Basil (Ocimum basilicum L.) is found worldwide and is used in the food, pharmaceutical, and cosmetic industries; however, the nutritional and functional properties of the seeds are scarcely known. Basil seeds contain high concentrations of proteins (11.4-22.5 g/100 g), with all the essential amino acids except S-containing types and tryptophan; dietary fiber (soluble and insoluble) ranging from 7.11 to 26.2 g/100 g lipids, with linoleic (12-85.6 g/100 g) and linolenic fatty acids (0.3-75 g/100 g) comprising the highest proportions; minerals, such as calcium, potassium, and magnesium, in high amounts; and phenolic compounds, such as orientine, vicentine, and rosmarinic acid. In addition, their consumption is associated with several health benefits, such as the prevention of type-2 diabetes, cardio-protection, antioxidant and antimicrobial effects, and anti-inflammatory, antiulcer, anticoagulant, and anti-depressant properties, among others. The focus of this systematic review was to study the current state of knowledge and explore the enormous potential of basil seeds as a functional food and source of functional ingredients to be incorporated into foods.

RESUMO

BACKGROUND: The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. METHODS: Fresh blossoms were extracted at low temperature (0 °C) using a watery solvent. Human MCF7 breast cancer cells were then treated with 3 separate fluctuated concentrations of 0, 50, 150 and 250 µg/mL for 24 and 48 h. RESULTS: The outcomes demonstrated the presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids and volatile oils and nonappearance of Terpinoids and alkaloids. Contrastingly, frail presence of steroids in basil blossoms aqueous concentrate was noted. In addition, the results from a phytochemical subjective examination of basil (Ocimum basilicum L.) blossoms aqueous extract demonstrated that most of the credited natural impacts containing more remarkable contents of antioxidants and anticancer compounds in basil blossoms aqueous extract. Moreover, the restraint of glucose take-up was alleviated mediated by a dose-dependent manner in MCF7 cells with basil (Ocimum basilicum L.) blossoms aqueous extract inducted for 24 h, resulting in mitochondrial fission. CONCLUSION: This is the first study that shows the impact of the aqueous extract of basil (Ocimum basilicum L.) blossoms was extracted at low temperature (0℃/6 h) underlined high amounts of flavonoids and phenolic compounds bearing more anticancer and antioxidant activities compared to another aqueous extract (using boiled water solvent) and alcoholic extracts.

Assuntos

RESUMO

BACKGROUND: The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. METHODS: Fresh blossoms were extracted at low temperature (0 °C) using a watery solvent. Human MCF7 breast cancer cells were then treated with 3 separate fluctuated concentrations of 0, 50, 150 and 250 µg/mL for 24 and 48 h. RESULTS: The outcomes demonstrated the presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids and volatile oils and nonappearance of Terpinoids and alkaloids. Contrastingly, frail presence of steroids in basil blossoms aqueous concentrate was noted. In addition, the results from a phytochemical subjective examination of basil (Ocimum basilicum L.) blossoms aqueous extract demonstrated that most of the credited natural impacts containing more remarkable contents of antioxidants and anticancer compounds in basil blossoms aqueous extract. Moreover, the restraint of glucose take-up was alleviated mediated by a dose-dependent manner in MCF7 cells with basil (Ocimum basilicum L.) blossoms aqueous extract inducted for 24 h, resulting in mitochondrial fission. CONCLUSION: This is the first study that shows the impact of the aqueous extract of basil (Ocimum basilicum L.) blossoms was extracted at low temperature (0°C/6 h) underlined high amounts of flavonoids and phenolic compounds bearing more anticancer and antioxidant activities compared to another aqueous extract (using boiled water solvent) and alcoholic extracts.

Assuntos

RESUMO

Sweet basil (Ocimum basilicum L.) is one of the most produced aromatic herbs in the world, exploiting hydroponic systems. It has been widely assessed that macronutrients, like nitrogen (N) and sulfur (S), can strongly affect the organoleptic qualities of agricultural products, thus influencing their nutraceutical value. In addition, plant-growth-promoting rhizobacteria (PGPR) have been shown to affect plant growth and quality. Azospirillum brasilense is a PGPR able to colonize the root system of different crops, promoting their growth and development and influencing the acquisition of mineral nutrients. On the bases of these observations, we aimed at investigating the impact of both mineral nutrients supply and rhizobacteria inoculation on the nutraceutical value on two different sweet basil varieties, i.e., Genovese and Red Rubin. To these objectives, basil plants have been grown in hydroponics, with nutrient solutions fortified for the concentration of either S or N, supplied as SO4 2- or NO3 -, respectively. In addition, plants were either non-inoculated or inoculated with A. brasilense. At harvest, basil plants were assessed for the yield and the nutraceutical properties of the edible parts. The cultivation of basil plants in the fortified nutrient solutions showed a general increasing trend in the accumulation of the fresh biomass, albeit the inoculation with A. brasilense did not further promote the growth. The metabolomic analyses disclosed a strong effect of treatments on the differential accumulation of metabolites in basil leaves, producing the modulation of more than 400 compounds belonging to the secondary metabolism, as phenylpropanoids, isoprenoids, alkaloids, several flavonoids, and terpenoids. The primary metabolism that resulted was also influenced by the treatments showing changes in the fatty acid, carbohydrates, and amino acids metabolism. The amino acid analysis revealed that the treatments induced an increase in arginine (Arg) content in the leaves, which has been shown to have beneficial effects on human health. In conclusion, between the two cultivars studied, Red Rubin displayed the most positive effect in terms of nutritional value, which was further enhanced following A. brasilense inoculation.

RESUMO

Aromatization of extra-virgin olive oil (EVOO) with aromatic plants is commonly used to enrich the oil with aromatic and antioxidant compounds. Ultrasound can be an alternative to accelerate this process. The objective of this work was to determine if ultrasound is able to accelerate EVOO aromatization with rosemary and basil and how it affects the migration of volatile and other compounds, the oxidative stability and the antioxidant capacity of the aromatized products. Ultrasound parameters (amplitude, time, and temperature of extraction) were optimized for each herb with central composite designs. Free fatty acid, peroxide value, K232, K270, ΔK, fatty acid profile, total phenolics, antioxidant capacity, polar compounds, oxidative stability and volatile compounds profile were evaluated in all samples. Physical effects of ultrasound on the herbs were observed by scanning electron microscopy. In the optimization, variables related to the oxidative processes were minimized and compounds migration and oxidative stability were maximized. Results were 70.09% amplitude, 36.6 min and 35 °C for rosemary and 95.98% amplitude, 9.9 min and 30 °C for basil. These conditions were compared to 7 and 15 days of conventional maceration (CM). Aromatization of EVOO with rosemary, both by ultrasound assisted maceration (UAM) or CM, improved total phenolics, terpenes, esters, ketones, stability and induction times, as well as decreased the values for the quality parameters. The use of UAM accelerated the process to 37 min. However, aromatization with basil by CM increased the values for the quality parameters and reduced the total phenolics, the antioxidant capacity and the induction and stability times. UAM with basil reached better results than those observed for CM, in only 10 min. In conclusion, rosemary is more appropriate than basil for EVOO aromatization, and UAM was the best choice to accelerate the processes when compared to CM.

Assuntos

RESUMO

Essential oils have emerged as an alternative to synthetic insecticides in the control of stored grain pests. The toxicity and repellency of the essential oils of four basil cultivars and three basil hybrids and the monoterpenes linalool, citral, and (E)-methyl cinnamate were evaluated in the stored grain pests Callosobruchus maculatus and Sitophillus zeamais. The essential oils of the cultivar Genovese and the hybrid 'Genovese' x 'Maria Bonita' were more toxic to C. maculatus. Conversely, the essential oils of the cultivar Sweet Dani and the hybrid 'Cinnamom' x 'Maria Bonita' were more toxic to S. zeamais. Among the monoterpenes, (E)-methyl cinnamate was the most toxic to both pests, taking 0.14 and 0.34 µ L.mL-1 to kill 50% of the C. maculatus and S. zeamais populations, respectively. All essential oils from cultivars, hybrids, and monoterpenes were repellent to S. zeamais, except for (E)-methyl cinnamate. For C. maculatus, this effect was lower, being citral the most repellent compound. Results demonstrate the insecticidal potential of the essential oil of O. basilicum and its monoterpenes in the control of stored grain pests.

Os óleos essenciais surgem como alternativa aos inseticidas sintéticos no controle das pragas de grãos armazenados. A toxicidade e a repelência dos óleos essenciais de quatro cultivares e três híbridos de manjericão e dos monoterpenos linalol, citral e (E)-cinamato de metila foram avaliadas nas pragas de grãos armazenados Callosobruchus maculatus e Sitophillus zeamais. Os óleos essenciais da cultivar Genovese e do híbrido 'Genovese' x 'Maria Bonita' foram mais tóxicos para C. maculatus. Já para S. zeamais, os óleos essenciais das cultivar Sweet Dani e do híbrido 'Cinnamom' x 'Maria Bonita' apresentaram maior toxicidade. Dentre os monoterpenos, o (E)-cinamato de metila foi o mais tóxico para ambas as pragas. Foram necessários 0,14 e 0,34 µ L.mL-1 para matar 50% da população de C. maculatus e S. zeamais. Todos os óleos essenciais das cultivares, dos híbridos e dos monoterpenos foram repelentes a S. zeamais, com exceção do (E)-cinamato de metila. Já para C. maculatus, este efeito foi reduzido, sendo o citral o composto mais repelente. Nos resultados demonstram o potencial inseticida dos óleos essenciais de O. basilicum e seus monoterpenos para o controle de pragas de grãos armazenados.

Assuntos

RESUMO

Natural antimicrobials, known as phytobiotics, are used in bacterial infections. The objective of this study was to evaluatethe phytobiotic activity, in vitro and in vivo, of an extract and an essential oil of Piper auritum and Ocimum basilicum on avian Eschericia coli serotype O2 in broiler chickens experimentally infected. For the in vitro test, extracts at 4, 8, 12 and 16% in water-based solvent or alcohol at 70% were prepared from leaves of both plants. In the essential oils, solvents at 10% were used. A concentration of 1×108 CFU mL-1 of bacteria was seeded and on each sense disc, 25 µL of the extract or essential oil were poured, except on the positive or negative control. The diameter of the inhibition zone (DIZ) of bacterial growth was measured. In the in vivo test, 40 chickens were inoculated, by intratracheal route, with a bacterial suspension of 1 × 108 CFU mL-1. E. col was identified and isolated from the organs; mortality, morbidity and relative weight of the organs were measured, and postmortem lesions and histopathologic findings were observed. A completely randomized design and the Kruskal-Wallis test for data analysis was used. By increasing the concentration of the extract, DIZ was greater; at the time of slaughter, differences in body weight (p 0.05) were found and the majority of lesions were observed in lungs. It is concluded that leave extracts of P. auritum and O. basilicum had phytobiotic activity on E. col serotype O2.

Assuntos

RESUMO

Natural antimicrobials, known as phytobiotics, are used in bacterial infections. The objective of this study was to evaluatethe phytobiotic activity, in vitro and in vivo, of an extract and an essential oil of Piper auritum and Ocimum basilicum on avian Eschericia coli serotype O2 in broiler chickens experimentally infected. For the in vitro test, extracts at 4, 8, 12 and 16% in water-based solvent or alcohol at 70% were prepared from leaves of both plants. In the essential oils, solvents at 10% were used. A concentration of 1×108 CFU mL-1 of bacteria was seeded and on each sense disc, 25 µL of the extract or essential oil were poured, except on the positive or negative control. The diameter of the inhibition zone (DIZ) of bacterial growth was measured. In the in vivo test, 40 chickens were inoculated, by intratracheal route, with a bacterial suspension of 1 × 108 CFU mL-1. E. col was identified and isolated from the organs; mortality, morbidity and relative weight of the organs were measured, and postmortem lesions and histopathologic findings were observed. A completely randomized design and the Kruskal-Wallis test for data analysis was used. By increasing the concentration of the extract, DIZ was greater; at the time of slaughter, differences in body weight (p 0.05) were found and the majority of lesions were observed in lungs. It is concluded that leave extracts of P. auritum and O. basilicum had phytobiotic activity on E. col serotype O2.(AU)

Assuntos

RESUMO

O manjericão (Ocimum basilicum L.) é uma erva com ampla aplicação na culinária e relevante valor nutricional. Neste contexto, este trabalho determinou a composição centesimal e a capacidade antioxidante de folhas de manjericão orgânico. Foi determinada a seguinte composição centesimal em base seca: proteínas 43,57 ± 0,35 g/100g, lipídios 17,58 ± 1,05 g/100g, carboidratos 17,14 ± 0,51 g/100g e cinzas 21,70 ± 0,08 g/100g. A capacidade antioxidante foi determinada pela quantificação dos fenólicos totais (18,10±0,17 mg AGE/g) e do percentual de desativação do radical DPPH (89,16%). Teores de clorofila e carotenoides totais também foram quantificados. Os resultados apontam o potencial nutricional do manjericão analisado, onde a presença de compostos bioativos pode caracterizar a atividade antioxidante observada.

Assuntos

RESUMO

O manjericão (Ocimum basilicum L.) é uma erva com ampla aplicação na culinária e relevante valor nutricional. Neste contexto, este trabalho determinou a composição centesimal e a capacidade antioxidante de folhas de manjericão orgânico. Foi determinada a seguinte composição centesimal em base seca: proteínas 43,57 ± 0,35 g/100g, lipídios 17,58 ± 1,05 g/100g, carboidratos 17,14 ± 0,51 g/100g e cinzas 21,70 ± 0,08 g/100g. A capacidade antioxidante foi determinada pela quantificação dos fenólicos totais (18,10±0,17 mg AGE/g) e do percentual de desativação do radical DPPH (89,16%). Teores de clorofila e carotenoides totais também foram quantificados. Os resultados apontam o potencial nutricional do manjericão analisado, onde a presença de compostos bioativos pode caracterizar a atividade antioxidante observada.(AU)

Assuntos