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The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium (Bacillus cabrialesii subsp. cabrialesii TE3T, Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha-1) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123-225 kg N ha-1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha-1 (6.0 vs. 7.1 ton ha-1, 0 kg N ha-1 added, 123 kg N ha-1 in the soil) and of 2.0 ton ha-1 (5.9 vs. 7.9 ton ha-1, 120 kg N ha-1 added, 104 kg N ha-1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha-1) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha-1 was applied compared to when wheat was fully fertilized (240 kg N ha-1). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat.

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Nitrogen (N), the most important macro-nutrient for plant growth and development, is a key factor that determines crop yield. Yet its excessive applications pollute the environment and are expensive. Hence, studying nitrogen use efficiency (NUE) in crops is fundamental for sustainable agriculture. Here, an association panel consisting of 123 flax accessions was evaluated for 21 NUE-related traits at the seedling stage under optimum N (N+) and N deficiency (N-) treatments to dissect the genetic architecture of NUE-related traits using a multi-omics approach integrating genome-wide association studies (GWAS), transcriptome analysis and genomic selection (GS). Root traits exhibited significant and positive correlations with NUE under N- conditions (r = 0.33 to 0.43, p < 0.05). A total of 359 QTLs were identified, accounting for 0.11% to 23.1% of the phenotypic variation in NUE-related traits. Transcriptomic analysis identified 1034 differentially expressed genes (DEGs) under contrasting N conditions. DEGs involved in N metabolism, root development, amino acid transport and catabolism and others, were found near the QTLs. GS models to predict NUE stress tolerance index (NUE_STI) trait were tested using a random genome-wide SNP dataset and a GWAS-derived QTLs dataset. The latter produced superior prediction accuracy (r = 0.62 to 0.79) compared to the genome-wide SNP marker dataset (r = 0.11) for NUE_STI. Our results provide insights into the QTL architecture of NUE-related traits, identify candidate genes for further studies, and propose genomic breeding tools to achieve superior NUE in flax under low N input.

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Sugarcane (Saccharum spp.) is an important crop for sugar and bioethanol production worldwide. To maintain and increase sugarcane yields in marginal areas, the use of nitrogen (N) fertilizers is essential, but N overuse may result in the leaching of reactive N to the natural environment. Despite the importance of N in sugarcane production, little is known about the molecular mechanisms involved in N homeostasis in this crop, particularly regarding ammonium (NH4 +), the sugarcane's preferred source of N. Here, using a sugarcane bacterial artificial chromosome (BAC) library and a series of in silico analyses, we identified an AMMONIUM TRANSPORTER (AMT) from the AMT2 subfamily, sugarcane AMMONIUM TRANSPORTER 3;3 (ScAMT3;3), which is constitutively and highly expressed in young and mature leaves. To characterize its biochemical function, we ectopically expressed ScAMT3;3 in heterologous systems (Saccharomyces cerevisiae and Arabidopsis thaliana). The complementation of triple mep mutant yeast demonstrated that ScAMT3;3 is functional for NH3/H+ cotransport at high availability of NH4 + and under physiological pH conditions. The ectopic expression of ScAMT3;3 in the Arabidopsis quadruple AMT knockout mutant restored the transport capacity of 15N-NH4 + in roots and plant growth under specific N availability conditions, confirming the role of ScAMT3;3 in NH4 + transport in planta. Our results indicate that ScAMT3;3 belongs to the low-affinity transport system (Km 270.9 µM; Vmax 209.3 µmol g-1 root DW h-1). We were able to infer that ScAMT3;3 plays a presumed role in NH4 + source-sink remobilization in the shoots via phloem loading. These findings help to shed light on the functionality of a novel AMT2-type protein and provide bases for future research focusing on the improvement of sugarcane yield and N use efficiency.

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Ammonium (NH4+) is an essential nitrogen source for plants, but excessive exposure can trigger stress responses that vary among and within different plant species. This study investigated the phenotypic variations in response to ammonium nutrition in five oil palm genotypes seedlings. Nitrate nutrition was used as a reference for a non-stressful condition, and three different nitrogen concentrations (5, 10, and 15 mM) were examined. Control groups without external nitrogen application were included for each genotype. Several parameters were analyzed, including plant growth, root length, gas exchange, fluorescence, chlorophyll, reducing sugars, amino acids, proteins, and nitrogen uptake. The results revealed a significant genotype effect, particularly between the interspecific OxG hybrid and the Elaeis guinensis genotypes. Ammonium nutrition increased shoot growth in all genotypes compared to nitrate nutrition. Additionally, there was a trend towards increased primary root length, amino acids, proteins, and nitrogen uptake under ammonium supply. These findings are promising, particularly considering the recommendation to use ammonium with inhibitors for environmental sustainability.

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Wheat is one of the staple foods of the global population due to its adaptability to a wide range of environments. Nitrogen is one of the crucial limiting factors in wheat production and is considered a challenge to food security. Therefore, sustainable agricultural technologies such as seed inoculation with plant growth-promoting bacteria (PGPBs) can be adopted to promote biological nitrogen fixation (BNF) for higher crop productivity. In this context, the objective of the current study was to evaluate the effects of nitrogen fertilization and seed inoculations with Azospirillum brasilense, Bacillus subtilis and A. brasilense + B. subtilis on agronomic and yield attributes, grain yield, grain N accumulation, N use efficiency and applied N recovery in Brazilian Cerrado, which consists of gramineous woody savanna. The experiment was carried out in two cropping seasons in Rhodic Haplustox soil under a no-tillage system. The experiment was designed in a randomized complete block in a 4 × 5 factorial scheme, with four replications. The treatments consisted of four seed inoculations (control-without inoculation, inoculation with A. brasilense, B. subtilis and A. brasilense + B. subtilis) under five N doses (0, 40, 80, 120 and 160 kg ha-1, applied from urea) at the wheat tillering stage. Seed co-inoculation with A. brasilense + B. subtilis increased grain N accumulation, number of spikes m-1, grains spike-1 and grain yield of wheat in an irrigated no-tillage system of tropical savannah, regardless of the applied N doses. Nitrogen fertilization at a dose of 80 kg ha-1 significantly increased grain N accumulation and number of grains spikes-1 and nitrogen use efficiency. Recovery of applied N was increased with inoculation of B. subtilis and co-inoculation of A. brasilense + B. subtilis at increasing N doses. Therefore, N fertilization can be reduced by the inclusion of co-inoculation with A. brasilense + B. subtilis in the cultivation of winter wheat under a no-tillage system of Brazilian Cerrado.

RESUMO

AMMONIUM TRANSPORTER/METHYLAMMONIUM PERMEASE/RHESUS (AMT) family members transport ammonium across membranes in all life domains. Plant AMTs can be categorized into AMT1 and AMT2 subfamilies. Functional studies of AMTs, particularly AMT1-type, have been conducted using model plants but little is known about the function of AMTs from crops. Sugarcane (Saccharum spp.) is a major bioenergy crop that requires heavy nitrogen fertilization but depends on a low carbon-footprint for competitive sustainability. Here, we identified and functionally characterized sugarcane ScAMT2;1 by complementing ammonium uptake-defective mutants of Saccharomyces cerevisiae and Arabidopsis thaliana. Reporter gene driven by the ScAMT2;1 promoter in A. thaliana revealed preferential expression in the shoot vasculature and root endodermis/pericycle according to nitrogen availability and source. Arabidopsis quadruple mutant plants expressing ScAMT2;1 driven by the CaMV35S promoter or by a sugarcane endogenous promoter produced significantly more biomass than mutant plants when grown in NH4 + and showed more 15N-ammonium uptake by roots and nitrogen translocation to shoots. In A. thaliana, ScAMT2;1 displayed a Km of 90.17 µM and Vmax of 338.99 µmoles h-1 g-1 root DW. Altogether, our results suggest that ScAMT2;1 is a functional high-affinity ammonium transporter that might contribute to ammonium uptake and presumably to root-to-shoot translocation under high NH4 + conditions.

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The sunflower is an oilseed species, rustic, cultivated in different agroecosystems. The responses of cultivars to nitrogen fertilization vary according to the cultivar and environment. Therefore, this research aimed to evaluate the response of biometric characters of sunflower cv. Embrapa 122-V2000 under N doses. The experiment was carried out in a randomized block design with five treatments (0, 40, 80, 120 and 160 kg N ha-1) and four blocks. All characters were influenced (p<0.05) by N doses, fitting to the quadratic model. The maximum values of plant height (1.68 m), stem diameter (22.71 mm), leaf area (423.41 cm2), capitulum diameter (15.22 cm), dry biomass yield (6,075.13 kg DM ha-1) and grains (2,982.89 kg ha-1), N use efficiency from biomass (80.3 kg kg-1) and grains (43.27 kg kg-1) close to 90 kg N ha-1, this dose being recommended for the edaphoclimatic conditions of the Agreste Meridional Region of Pernambuco, Brazil.(AU)

O girassol é uma espécie oleaginosa, rústica, cultivada em diferentes agroecossistemas. As respostas dos cultivares às adubações nitrogenadas variam de acordo com o cultivar e o ambiente. Portanto, esta pesquisa objetivou avaliar a resposta de caracteres biométricos do girassol cv. Embrapa 122-V2000 sob doses de N. O experimento foi conduzido no delineamento em blocos casualizados com cinco tratamentos (0, 40, 80, 120 e 160 kg N ha-1) e quatro blocos. Todos os caracteres foram influenciados (p<0,05) pelas doses de N, ajustando-se ao modelo quadrático. Os valores máximos de altura da planta (1,68 m), diâmetro do colmo (22,71 mm), área foliar (423,41 cm2), diâmetro do capítulo (15,22 cm), produtividade de biomassa seca (6.075,13 kg MS ha-1) e de grãos (2.982,89 kg ha-1), eficiência do uso do N da biomassa (80,3 kg kg-1) e dos grãos (43,27 kg kg-1) próximo aos 90 kg N ha-1, sendo esta dose recomendada para as condições edafoclimáticas da região Agreste Meridional de Pernambuco, Brasil.(AU)

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KEY MESSAGE: Nitrate uptake in sugarcane roots is regulated at the transcriptional and posttranscriptional levels based on the physiological status of the plant and is likely a determinant mechanism for discrimination against nitrate. Sugarcane (Saccharum spp.) is one of the most suitable energy crops for biofuel feedstock, but the reduced recovery of nitrogen (N) fertilizer by sugarcane roots increases the crop carbon footprint. The low nitrogen use efficiency (NUE) of sugarcane has been associated with the significantly low nitrate uptake, which limits the utilization of the large amount of nitrate available in agricultural soils. To understand the regulation of nitrate uptake in sugarcane roots, we identified the major canonical nitrate transporter genes (NRTs-NITRATE TRANSPORTERS) and then determined their expression profiles in roots under contrasting N conditions. Correlation of gene expression with 15N-nitrate uptake revealed that under N deprivation or inorganic N (ammonium or nitrate) supply in N-sufficient roots, the regulation of ScNRT2.1 and ScNRT3.1 expression is the predominant mechanism for the modulation of the activity of the nitrate high-affinity transport system. Conversely, in N-deficient roots, the induction of ScNRT2.1 and ScNRT3.1 transcription is not correlated with the marked repression of nitrate uptake in response to nitrate resupply or high N provision, which suggested the existence of a posttranscriptional regulatory mechanism. Our findings suggested that high-affinity nitrate uptake is regulated at the transcriptional and presumably at the posttranscriptional levels based on the physiological N status and that the regulation of NRT2.1 and NRT3.1 activity is likely a determinant mechanism for the discrimination against nitrate uptake observed in sugarcane roots, which contributes to the low NUE in this crop species.

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High crop yields are generally associated with high nitrogen (N) fertilizer rates. A growing tendency that is urgently demanding the adoption of precision technologies that manage N more efficiently, combined with the advances of crop genetics to meet the needs of sustainable farm systems. Among the plant traits, stem architecture has been of paramount importance to enhance harvest index in the cereal crops. Nonetheless, the reduced stature also brought undesirable effect, such as poor N-uptake, which has led to the overuse of N fertilizer. Therefore, a better understanding of how N signals modulate the initial and late stages of stem development might uncover novel semi-dwarf alleles without pleiotropic effects. Our attempt here is to review the most recent advances on this topic.

RESUMO

Nitrogen is the main limiting nutrient after carbon, hydrogen and oxygen for photosynthetic process, phyto-hormonal, proteomic changes and growth-development of plants to complete its lifecycle. Excessive and inefficient use of N fertilizer results in enhanced crop production costs and atmospheric pollution. Atmospheric nitrogen (71%) in the molecular form is not available for the plants. For world's sustainable food production and atmospheric benefits, there is an urgent need to up-grade nitrogen use efficiency in agricultural farming system. The nitrogen use efficiency is the product of nitrogen uptake efficiency and nitrogen utilization efficiency, it varies from 30.2 to 53.2%. Nitrogen losses are too high, due to excess amount, low plant population, poor application methods etc., which can go up to 70% of total available nitrogen. These losses can be minimized up to 15-30% by adopting improved agronomic approaches such as optimal dosage of nitrogen, application of N by using canopy sensors, maintaining plant population, drip fertigation and legume based intercropping. A few transgenic studies have shown improvement in nitrogen uptake and even increase in biomass. Nitrate reductase, nitrite reductase, glutamine synthetase, glutamine oxoglutarate aminotransferase and asparagine synthetase enzyme have a great role in nitrogen metabolism. However, further studies on carbon-nitrogen metabolism and molecular changes at omic levels are required by using "whole genome sequencing technology" to improve nitrogen use efficiency. This review focus on nitrogen use efficiency that is the major concern of modern days to save economic resources without sacrificing farm yield as well as safety of global environment, i.e. greenhouse gas emissions, ammonium volatilization and nitrate leaching.

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The use of Azospirillum brasilense has the potential to improve plant nitrogen (N) use efficiency, while a better understanding of alternative management practices with inoculation is necessary. The aim was to examine the effects of the leaf application of A. brasilense in association with nitrogen fertilization on the wheat crop. The experiment was conducted in Lidianópolis, Paraná, Brazil, in a completely randomized block design with four replications. The treatments included four doses of A. brasilense for leaf application (0, 200, 400, and 600 ml ha-1) and four doses of N (0, 40, 80, and 120 kg N ha-1). The nutritional content, yield components, quality, and yield of the wheat crop were evaluated. There was no interaction among the factors, nor did the treatments have an isolated effect on spike length, the number of spikelets per spike, spikes per m2, thousand grain weight, and test weight. However, doses of A. brasilense increased calcium and magnesium absorption at 283 and 380 ml ha-1, respectively. Similarly, nitrogen application increased the content of calcium, magnesium, and copper in the leaf at 61, 47, and 49 kg N ha-1, respectively. Nitrogen also increased the number of grains per spike and yield at 56 and 54 kg N ha-1, respectively. Yield correlated with the number of grains per spike and the manganese and copper content in the leaf.

O uso de Azospirillum brasilense apresenta potencial em melhorar a eficiência de uso do nitrogênio (N), sendo necessário melhor compreensão de formas alternativas de inoculação, visto que o tratamento químico de sementes pode comprometer a eficiência das bactérias. O objetivo foi avaliar os efeitos do A. brasilense aplicado via foliar associado a adubação nitrogenada na cultura do trigo. O experimento foi implantado em Lidianópolis, no estado do Paraná, Brasil, em blocos completos com tratamentos ao acaso e quatro repetições, sendo os tratamentos: quatro doses de A. brasilense via foliar (0, 200, 400 e 600 ml ha-1) e quatro doses de N (0, 40, 80 e 120 kg N ha-1). Foram avaliados os teores nutricionais, componentes de rendimento, qualidade e produtividade. Não houve interação entre os fatores, tampouco efeito isolado dos tratamentos para comprimento da espiga, número de espiguetas por espiga, espigas por m², massa de mil grãos e peso de hectolitro. Todavia as doses de A. brasilense, aumentaram a absorção de Ca e Mg até a dose de 283 e 380 ml ha-1, respectivamente. De modo similar, o N aplicado aumentou os teores foliares de cálcio, magnésio, além de cobre até a dose de 61, 47 e 49 kg ha-1 de N, respectivamente. O N também incrementou o número de grãos por espiga e a produtividade até a dose 56 e 54 kg N ha-1, respectivamente, porém a eficiência de uso

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O objetivo do presente estudo foi avaliar a resposta agronômica e econômica da pastagem de aveia preta (cv. Embrapa 29 Garoa) submetida a diferentes fontes e doses de fertilização nitrogenada. O delineamento experimental utilizado foi o de blocos ao acaso, organizado em esquema fatorial: duas fontes de Nitrogênio x cinco doses de fertilização x três cortes. Trezentos resultados obtidos demonstraram que a cultura da aveia preta responde em potencial produtivo a partir de diferentes fontes e doses de fertilização nitrogenada, impactando de maneira diferenciada sobre o produto animal. A escolha da dose de fertilização dentro das distintas fontes de Nitrogênio depende da eficiência de utilização do Nitrogênio em produção de forragem e a sua conversão em produção animal, aliado à remuneração do produto e custos envolvidos com a implantação da pastagem. A aplicação de ureia como fonte de Nitrogênio proporcionou maior produção de matéria seca por corte na cultura de aveia preta quando comparado ao sulfato de amônio. As maiores eficiências de utilização de nitrogênio foram obtidas com a aplicação de 45 kg ha-1 de ureia e sulfato de amônio. A cultura de aveia preta responde biologicamente até a dose de fertilização nitrogenada de 90 kg de N ha-1. As aplicações de 45 kg de N ha-1 na forma de ureia e 23 kg ha-1 de sulfato de amônio foram economicamente viáveis nas condições em que o estudo foi realizado.(AU)

The objective of this study was to evaluate the agronomic and economic response of black oat pasture (cv. Embrapa 29 Garoa) submitted to different nitrogen fertilization sources and doses. The experimental design used was a randomized block design, arranged in a factorial scheme: two sources of nitrogen x five fertilization doses x three cuts. Three hundred results obtained demonstrated that black oat crop responds in productive potential from different sources and doses of nitrogen fertilization, impacting differently on the animal product. The choice of fertilization dose within the different sources of nitrogen will depend on the efficiency of nitrogen utilization in fodder production and its conversion to animal production, coupled with the product remuneration and costs involved with pasture implantation. The application of urea as nitrogen source provided greater production of dry matter per cut in black oat culture when compared to ammonium sulfate. The highest efficiencies in the use of nitrogen were obtained by applying 45 kg ha-1 of urea and ammonium sulfate. Black oat culture responds biologically to the nitrogen fertilization dose of 90 kg N ha-1. The applications of 45 kg of N ha-1 in the form of urea and 23 kg ha-1 of ammonium sulfate were economically feasible under the conditions in which the study was carried out.(AU)

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RESUMO

O objetivo do presente estudo foi avaliar a resposta agronômica e econômica da pastagem de aveia preta (cv. Embrapa 29 Garoa) submetida a diferentes fontes e doses de fertilização nitrogenada. O delineamento experimental utilizado foi o de blocos ao acaso, organizado em esquema fatorial: duas fontes de Nitrogênio x cinco doses de fertilização x três cortes. Trezentos resultados obtidos demonstraram que a cultura da aveia preta responde em potencial produtivo a partir de diferentes fontes e doses de fertilização nitrogenada, impactando de maneira diferenciada sobre o produto animal. A escolha da dose de fertilização dentro das distintas fontes de Nitrogênio depende da eficiência de utilização do Nitrogênio em produção de forragem e a sua conversão em produção animal, aliado à remuneração do produto e custos envolvidos com a implantação da pastagem. A aplicação de ureia como fonte de Nitrogênio proporcionou maior produção de matéria seca por corte na cultura de aveia preta quando comparado ao sulfato de amônio. As maiores eficiências de utilização de nitrogênio foram obtidas com a aplicação de 45 kg ha-1 de ureia e sulfato de amônio. A cultura de aveia preta responde biologicamente até a dose de fertilização nitrogenada de 90 kg de N ha-1. As aplicações de 45 kg de N ha-1 na forma de ureia e 23 kg ha-1 de sulfato de amônio foram economicamente viáveis nas condições em que o estudo foi realizado.

The objective of this study was to evaluate the agronomic and economic response of black oat pasture (cv. Embrapa 29 Garoa) submitted to different nitrogen fertilization sources and doses. The experimental design used was a randomized block design, arranged in a factorial scheme: two sources of nitrogen x five fertilization doses x three cuts. Three hundred results obtained demonstrated that black oat crop responds in productive potential from different sources and doses of nitrogen fertilization, impacting differently on the animal product. The choice of fertilization dose within the different sources of nitrogen will depend on the efficiency of nitrogen utilization in fodder production and its conversion to animal production, coupled with the product remuneration and costs involved with pasture implantation. The application of urea as nitrogen source provided greater production of dry matter per cut in black oat culture when compared to ammonium sulfate. The highest efficiencies in the use of nitrogen were obtained by applying 45 kg ha-1 of urea and ammonium sulfate. Black oat culture responds biologically to the nitrogen fertilization dose of 90 kg N ha-1. The applications of 45 kg of N ha-1 in the form of urea and 23 kg ha-1 of ammonium sulfate were economically feasible under the conditions in which the study was carried out.

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The use of Azospirillum brasilense has the potential to improve plant nitrogen (N) use efficiency, while a better understanding of alternative management practices with inoculation is necessary. The aim was to examine the effects of the leaf application of A. brasilense in association with nitrogen fertilization on the wheat crop. The experiment was conducted in Lidianópolis, Paraná, Brazil, in a completely randomized block design with four replications. The treatments included four doses of A. brasilense for leaf application (0, 200, 400, and 600 ml ha-1) and four doses of N (0, 40, 80, and 120 kg N ha-1). The nutritional content, yield components, quality, and yield of the wheat crop were evaluated. There was no interaction among the factors, nor did the treatments have an isolated effect on spike length, the number of spikelets per spike, spikes per m2, thousand grain weight, and test weight. However, doses of A. brasilense increased calcium and magnesium absorption at 283 and 380 ml ha-1, respectively. Similarly, nitrogen application increased the content of calcium, magnesium, and copper in the leaf at 61, 47, and 49 kg N ha-1, respectively. Nitrogen also increased the number of grains per spike and yield at 56 and 54 kg N ha-1, respectively. Yield correlated with the number of grains per spike and the manganese and copper content in the leaf.(AU)

O uso de Azospirillum brasilense apresenta potencial em melhorar a eficiência de uso do nitrogênio (N), sendo necessário melhor compreensão de formas alternativas de inoculação, visto que o tratamento químico de sementes pode comprometer a eficiência das bactérias. O objetivo foi avaliar os efeitos do A. brasilense aplicado via foliar associado a adubação nitrogenada na cultura do trigo. O experimento foi implantado em Lidianópolis, no estado do Paraná, Brasil, em blocos completos com tratamentos ao acaso e quatro repetições, sendo os tratamentos: quatro doses de A. brasilense via foliar (0, 200, 400 e 600 ml ha-1) e quatro doses de N (0, 40, 80 e 120 kg N ha-1). Foram avaliados os teores nutricionais, componentes de rendimento, qualidade e produtividade. Não houve interação entre os fatores, tampouco efeito isolado dos tratamentos para comprimento da espiga, número de espiguetas por espiga, espigas por m², massa de mil grãos e peso de hectolitro. Todavia as doses de A. brasilense, aumentaram a absorção de Ca e Mg até a dose de 283 e 380 ml ha-1, respectivamente. De modo similar, o N aplicado aumentou os teores foliares de cálcio, magnésio, além de cobre até a dose de 61, 47 e 49 kg ha-1 de N, respectivamente. O N também incrementou o número de grãos por espiga e a produtividade até a dose 56 e 54 kg N ha-1, respectivamente, porém a eficiência de uso(AU)

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Grazing ruminant systems can be sustainably intensified by improving efficiency while reducing their environmental impact. The objective of the present study was to examine the potential of pastures differing in water-soluble carbohydrates (WSC) and crude protein (CP) contents to affect milk production and composition as well as the behaviour of cows grazing perennial ryegrass (PRG) swards. By modifying the nitrogen (N) fertilisation rate (83 and 250 kg/ha per year) and the defoliation frequency (two or three leaves per tiller) in combination with cultivar selection (high-sugar vs. standard cultivars), we obtained two swards differing in WSC and CP contents. The two contrasting swards were each grazed by six dairy cows in nine daily strips in autumn. Pasture samples were collected to determine herbage mass and quality. Cow behaviour was recorded by direct observation. Herbage offered and apparently consumed were similar between swards (averaging 37.3 and 18.2 kg/cow, respectively), although the residual was lower in the high-sugar sward (1735 vs. 2143 kg/ha). Cows spent less time grazing in the high-sugar sward (66.9% v. 71.6%), but the rumination times was similar (14.6%). Milk production and composition were similar between groups, suggesting that high-quality pastures would require a greater difference in nutritional composition to affect animal performance.

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Plants from the Proteaceae family can thrive in old, impoverished soil with extremely low phosphorus (P) content, such as those typically found in South Western Australia (SWA) and South Africa. The South Western (SW) Australian Proteaceae species have developed strategies to deal with P scarcity, such as the high capacity to re-mobilize P from senescent to young leaves and the efficient use of P for carbon fixation. In Southern South America, six Proteaceae species grow in younger soils than those of SWA, with a wide variety of climatic and edaphic conditions. However, strategies in the nutrient use efficiency of Southern South (SS) American Proteaceae species growing in their natural ecosystems remain widely unknown. The aim of this study was to evaluate nutrient resorption efficiency and the photosynthetic nutrients use efficiency by SS American Proteaceae species, naturally growing in different sites along a very extensive latitudinal gradient. Mature and senescent leaves of the six SS American Proteaceae species (Embothrium coccineum, Gevuina avellana, Orites myrtoidea Lomatia hirsuta, L. ferruginea, and L. dentata), as well as, soil samples were collected in nine sites from southern Chile and were subjected to chemical analyses. Nutrient resorption (P and nitrogen) efficiency in leaves was estimated in all species inhabiting the nine sites evaluated, whereas, the photosynthetic P use efficiency (PPUE) and photosynthetic nitrogen (N) use efficiency (PNUE) per leaf unit were determined in two sites with contrasting nutrient availability. Our study exhibit for the first time a data set related to nutrient use efficiency in the leaves of the six SS American Proteaceae, revealing that for all species and sites, P and N resorption efficiencies were on average 47.7 and 50.6%, respectively. No correlation was found between leaf nutrient (P and N) resorption efficiency and soil attributes. Further, different responses in PPUE and PNUE were found among species and, contrary to our expectations, a higher nutrient use efficiency in the nutrient poorest soil was not found. We conclude that SS American Proteaceae species did not show a general pattern in the nutrient use efficiency among them neither with others Proteaceae species reported in the literature.

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AIMS: Symbiotic nitrogen fixation (SNF) contributes to improve grain yield under nitrogen (N) deficiency. Climbing beans are known to be superior to bush beans in their potential for SNF. The main objectives of this study were to: (i) quantify genotypic differences in SNF ability of climbing beans using 15N natural abundance method; (ii) identify climbing bean genotypes that combine high SNF ability with high yield potential that could serve as parents in the breeding program; and (iii) test whether δ15N in seed can be used instead of δ15N in shoot for estimating SNF ability. METHODS: 98 Climbing bean genotypes were evaluated for SNF ability in terms of nitrogen derived from the atmosphere (%Ndfa). Field trials were conducted at two locations in Colombia. RESULTS: Significant genotypic differences were observed in SNF ability. Good yielding lines with 4.6 t ha-1 fixed as much as 60% of their N (up to 92 kg of N fixed ha-1) without application of N fertilizer to soil. CONCLUSIONS: Based on evaluations from both locations, seven climbing bean lines (ENF 235, ENF 234, ENF 28, ENF 21, MAC 27, CGA 10 and PO07AT49) were identified as promising genotypes. Seed samples can be used to determine SNF ability, to select for genotypes with superior SNF ability.

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ABSTRACT Corn grain yield, nitrogen (N) fertilizer efficiency and distribution to corn alone and three forms of corn and palisadegrass (Urochloa spp.) intercropping implantation was investigated. A field experiment with 15N labeling fertilizer was performed in randomized block design. No form of palisadegrass intercropping implantation affected corn grain yield, total N accumulation and N use efficiency (NUE), which were 8.7 t ha-1, 205 kg ha-1 and 37% respectively. The palisadegrass produced on average 1.9 t of dry mass, absorbing a maximum of 6 kg ha-1 or 5.5% of N fertilizer during corn growing. Furthermore, the palisadegrass did not affect N fertilizer distribution in soil-plant system, in which 28.2% was recovered in the soil and 40.4% in the plants (corn + palisadegrass). The results show that for the three intercropping implantation methods the palisadegrass did not compete with corn for N fertilizer.

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RESUMO

The production capacity of green and dry mass of the entire plant, efficiency of N conversion, apparent N recovery and the chemical composition of cultivar Mulato II was evaluated under a system of cuts and nitrogen doses. The assay, conducted in the municipality of Goiânia, GO, Brazil, had a totally randomized 2 x 4 factorial design (2 height cuts, 0.40 and 0.50 m and 4 nitrogen doses), with three replications and subdivided subplots. Treatments comprised four N doses (0, 50, 100 and 150 kgha-1 N, with urea as nitrogen source). There was no significant interaction (p>0.05) between N doses and cut heights for the variables productivity of green (PGM) and dry (PDM) mass, nitrogen use efficiency (NUE) and apparent N recovery (ANR), which were neither affected (p<0.05) by N doses nor by evaluated cut heights. Average productivity reached 59,450 kgha-1 (PGV) and 10,367 kgha-1 (PDM) and it was produced an average of 19.62 kg of DM per kg of N, with a mean 56.00% recovery. N doses and cut heights did not affect (p>0.05) DM rates of the plant, whilst mean dry matter rate was 17.49%. CP rates were affected (p<0.05) by N doses (0, 50, 100 and 150 kgha-1) and cut heights (0.40 and 0.50 m) and by the interaction of these factors. CP rates of the entire plant hybrid Brachiaria cv. Mulato II increased (p<0.05) due to N doses through an increasing linear relationship. Since there was a significant effect (p<0.05) with regard to cut height and CP rates decreased with height increase. NDF rates were significantly influenced by N doses (p<0.05) and by cut heights (p<0.05), with significance for the interaction (p<0.05) of over 100 kgha-1 N doses only. No significant interaction (p>0.05) occurred in ADF rates among the variables analyzed. ADF contents were influenced by N supply (p<0.05) with decreasing quadratic regression as N doses increased.

O presente trabalho de pesquisa teve como objetivo avaliar o potencial produtivo de massa verde e seca da planta inteira, a eficiência de conversão do N, a recuperação aparente do N, bem como a composição bromatológica do cultivar Mulato II, sob regime de cortes e submetido a doses de nitrogênio, no município de Goiânia, GO, Brasil. Utilizou-se um delineamento experimental inteiramente casualizado com arranjo fatorial 2 X 4 (2 alturas de corte (0,40 e 0,50 m) X 4 doses de nitrogênio) com três repetições e parcelas subdivididas. Os tratamentos foram constituídos por quatro doses de N (0, 50, 100 e 150 kgha-1 de N) (sendo a fonte ureia). Não ocorreu interação significativa (p>0,05) entre doses de N e alturas de corte para as variáveis produtividades de massa verde (PMV) e massa seca (PMS), eficiência de conversão aparente de nitrogênio (ECAN) e recuperação aparente de nitrogênio (RAN), que não foram influenciadas (p<0,05) pelas doses de N, nem em função das alturas de corte avaliadas. A média de produtividade encontrada foi de 59.450 kgha-1 (PMV) e 10.367 kgha-1 (PMS), produzindo em média 19,62 kg de MS para cada kg de N aplicado, com uma recuperação média de 56,00%. As doses de N aplicadas e as alturas de corte não influenciaram (p>0,05) os teores de MS da planta. A média do teor de matéria seca encontrada foi de 17,49%. Os teores de PB foram influenciados (p<0,05) pelas doses de N (0, 50, 100 e 150 kgha-1) e alturas de corte (0,40 e 0,50 m) bem como a interação desses fatores. Os teores de proteína bruta (PB) da planta inteira Brachiaria híbrida cv. Mulato II aumentaram (p<0,05) em função das doses de N, apresentando uma relação linear crescente. Quanto à altura de corte, ocorreu também efeito significativo (p<0,05), evidenciando que os teores de PB diminuíram com o aumento da altura. Os teores de FDN foram influenciados significativamente pelas doses de N (p<0,05) e pelas alturas de corte (p<0,05), apresentando significância para a interação (p<0,05) apenas nas doses acima de 100 kgha-1 de N. Para os teores de FDA não houve interação significativa (p>0,05) entre as variáveis analisadas. O conteúdo de FDA foi influenciado pelo fornecimento de N (p<0,05), apresentando regressão quadrática decrescente com o aumento das doses de N.

Assuntos