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The objective of this study was to evaluate the effect of sweet potato vines as a source of fiber on the plasma parameters in healthy rabbits. For this, 15 rabbits were allocated into five groups, and each group was fed a different diet for a period of 46 days. The diets were as follows: diet without sweet potato vines (0SPV), and experimental diets with 25%, 50%, 75% and 100% of sweet potato vines in replacement of alfalfa hay respectively (25SPV, 50SPV, 75SPV and 100SPV). Triglycerides and VLDL-cholesterol concentrations were approximately 53% lower in rabbits fed the 100SPV than in rabbits fed 0SPV. In addition, aspartate aminotransferase (AST) and total-cholesterol concentrations were lower approximately 40% and 14%, respectively, in rabbits fed the 100SPV than in rabbits fed 0SPV. No significant differences were found among HDL-cholesterol, glucose, proteins, albumin and alanine aminotransferase (ALT) concentrations of the five groups. These results demonstrate that the consumption of sweet potato vines reduces the triglycerides, VLDLcholesterol and total cholesterol while maintains HDL-cholesterol levels. Therefore, sweet potato vines consumption may be another option to prevent coronary heart diseases in rabbits(AU)

O objetivo deste estudo foi avaliar com base no plasma sanguíneo o efeito do baraço de batata-doce como fonte de fibra. Para tal, dividiuse 15 coelhos em cinco grupos, onde cada grupo foi alimentado com uma dieta diferente por 46 dias. As dietas foram: sem baraço de batata-doce (0SPV), e dietas experimentais com 25%, 50%, 75% e 100% de baraço de batata-doce em substituição ao feno de alfafa, respectivamente (25SPV, 50SPV, 75SPV e 100SPV). As concentrações de triglicérides e VLDL-colesterol foram aproximadamente 53% menores em coelhos alimentados com a dieta 100SPV do que os coelhos alimentados com 0SPV. Além disso, as concentrações de aspartato aminotransferase (AST) e colesterol total foram inferiores em aproximadamente 40% e 14%, respectivamente, nos coelhos alimentados com 100 SPV do que em coelhos alimentados com 0SPV. Não foram encontradas diferenças significativas entre as concentrações colesterol-HDL, glicose, proteínas, albumina e alanina aminotransferase (ALT) dos cinco grupos. Estes resultados demonstram que o consumo de baraço de batata-doce reduz os triglicerídeos, colesterol VLDL e colesterol total, enquanto mantém os níveis de colesterol HDL. Portanto, o baraço de batata-doce pode ser uma opção viável para prevenir doenças coronarianas(AU)

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The aim of this study was to evaluate the histopathological changes in a model of New Zealand white rabbits after the ocular instillation of a fixed combination containing 0.09 % xanthan gum and 0.1 % chondroitin sulfate for 15 days. This was a preclinical study which compared the previously described combination with placebo on an animal model of sixteen New Zealand white rabbits. The intervention consisted of the instillation of one drop of a fixed combination containing 0.09 % xanthan gum and 0.1 % chondroitin sulfate on the right eye of each rabbit 6 times per day (every 2 h) in a 12-hour period for 15 days. The left eyes were used as control. Assessments on the anterior and posterior segment, ocular signs, and specific ocular surface characteristics were conducted at days 0, 1, 2, 4, 7, 10, and 15. Histopathological studies of the corneal and conjunctival epithelium were performed at the end of the intervention. No significant changes were observed during the assessments of the anterior and posterior segments. No differences in the number of goblet cells between groups were reported. The histopathological study of the corneal basement membrane's thickness, stromal, and conjunctival epithelium did not show significant changes between groups. The use of a fixed-dose combination containing 0.09 % xanthan gum and 0.1 % chondroitin sulfate on the ocular surface of New Zealand white rabbits every 2 h for 15 days causes no histopathological changes in anterior/posterior segments, nor a decrease in the number of goblet cells compared with placebo.

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The origin and distribution of the femoral nerves in both antimeres were studied in 30 New Zealand rabbits (15 males and 15 females). The specimens were collected after natural death and fixed with 10% formaldehyde solution. In males, the femoral nerve originated from the ventral branches of the fourth, sixth and seventh lumbar spinal nerves in seven animals (46.67%), in two animals (13.33%) from the ventral branches of the fifth and sixth lumbar spinal nerves, in one animal (6.67%) from the ventral branch of the fifth lumbar spinal nerve, in three animals (20%) from the ventral branches of the fifth, sixth and seventh lumbar spinal nerves, in one animal (6.67%) from the ventral branches of the fourth and fifth lumbar spinal nerves, and in one animal (6.67%) from the ventral branches of the fifth and seventh lumbar spinal nerves. In females, the femoral nerve originated from the ventral branches of the fourth, sixth and seventh lumbar spinal nerves in four animals (26.67%), in two cases (13.33%) from the ventral branches of the fifth and sixth lumbar spinal nerves, in one animal (6.67%) from the ventral branch of the fifth lumbar spinal nerve, in three animals (20%) from the ventral branches of the sixth and seventh lumbar spinal nerves, in four animals (26.67%) from the ventral branches of the fifth, sixth and seventh lumbar spinal nerves and in one animal (6.67%) from the ventral branches of the fourth and seventhlumbar spinal nerves. In all animals the femoral nerves were distributed in different branches to the major and minor psoas, femoral quadriceps, sartorius and pectinius muscles.

Se estudió el origen y distribución del nervio femoral de ambos antímeros en 30 conejos neozelandeses, 15 machos y 15 hembras. Los animales fueron recolectados después de su muerte natural y se fijaron en formaldehído al 10%. En los machos, el nervio femoral se originó a partir de los ramos ventrales del cuarto, sexto y séptimo nervios espinales lumbares en siete casos (46,67%); en tres casos (20%) desde los ramos ventrales del quinto, sexto y séptimo nervios espinales lumbares; en dos casos (13,33%) desde los ramos ventrales del quinto y sexto nervios espinales lumbares, mientras que en tres animales (n=1 respectivamente), desde los ramos ventrales del quinto nervio espinal lumbar (6,67%), los ramos ventrales del cuarto y quinto nervios lumbares espinales (6,67%) y desde los ramos ventrales del quinto y séptimo nervios espinales lumbares. En las hembras, el nervio femoral se originó a partir de los ramos ventrales del cuarto, sexto y séptimo nervios espinales en cuatro casos (26,67%); en otros cuatro casos (26,67%) desde los ramos ventrales del quinto, sexto y séptimo nervios espinales lumbar, en tres casos (20%) desde los ramos ventrales del sexto y séptimo nervios espinales lumbares, en dos casos (13,33%) desde los ramos ventrales del quinto y sexto nervios espinales, y en dos animales (n=1, respectivamente) procedían desde los ramos ventrales del quinto nervio espinal lumbar (6,67%) y de los ramos ventrales del cuarto y séptimo nervios espinales lumbares (6,67%). Los nervios femorales en todos los animales estaban distribuidos en diversos ramos de los músculos psoas mayor y menor, cuádriceps femoral sartorios y pectinatos.

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The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.(AU)

O objetivo deste estudo foi estabelecer valores de referência para testes diagnósticos oftálmicos em coelhos da raça Nova Zelândia (Oryctolagus cuniculus). 22 coelhos, machos, adultos foram utilizados. Foi mensurada a produção lacrimal através do teste lacrimal de Shirmer 1 (TLS1) e da Tira endodôntica de papel absorvente (EAPPTT) de duas marcas comerciais distintas. Tonometria de aplanação, identificação da microbiota conjuntival, , citologia e histologia conjuntival também foram realizadas. A média e desvio padrão do TLS1, EAPPTT1, EAPPTT2 e pressão intraocular foi 7,27±2,51 mm/min, 12,43±1,69 mm/min, 15,24±2,07 mm/min e 12,89±2,80 mmHg, respectivamente. Staphylococcus epidermidis, Staphylococcus sp. e Bacillus sp. mostraram-se predominantes. A citologia conjuntival evidenciou a presença de células epiteliais colunares, células escamosas superficiais queratinizadas, linfócitos, heterofilos, células sanguíneas, muco e bactérias. A histologia revelou epitélio estratificado caracterizado pela presença de células epiteliais colunares com grande número de células caliciformes. Os achados deste estudo poderão ser utilizados com fins terapêuticos ou experimentais.(AU)

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The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.

O objetivo deste estudo foi estabelecer valores de referência para testes diagnósticos oftálmicos em coelhos da raça Nova Zelândia (Oryctolagus cuniculus). 22 coelhos, machos, adultos foram utilizados. Foi mensurada a produção lacrimal através do teste lacrimal de Shirmer 1 (TLS1) e da Tira endodôntica de papel absorvente (EAPPTT) de duas marcas comerciais distintas. Tonometria de aplanação, identificação da microbiota conjuntival, , citologia e histologia conjuntival também foram realizadas. A média e desvio padrão do TLS1, EAPPTT1, EAPPTT2 e pressão intraocular foi 7,27±2,51 mm/min, 12,43±1,69 mm/min, 15,24±2,07 mm/min e 12,89±2,80 mmHg, respectivamente. Staphylococcus epidermidis, Staphylococcus sp. e Bacillus sp. mostraram-se predominantes. A citologia conjuntival evidenciou a presença de células epiteliais colunares, células escamosas superficiais queratinizadas, linfócitos, heterofilos, células sanguíneas, muco e bactérias. A histologia revelou epitélio estratificado caracterizado pela presença de células epiteliais colunares com grande número de células caliciformes. Os achados deste estudo poderão ser utilizados com fins terapêuticos ou experimentais.

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O objetivo do estudo foi realizar uma avaliação histomorfométrica dos tecidos formados nos alvéolos de extração de coelhos, preenchidos com dois diferentes tipos de biomateriais, em diferentes períodos de cicatrização. Material e Métodos: foram utlizados 14 coelhos da espécie Nova Zelândia, onde foram extraídos os dois molares do mesmo lado da mandíbula de cada animal. Um alvéolo foi preenchido com osso mineral bovino (Bio-Oss, grupo A) e o outro com uma hidroxiapatita sintética (Alobone poros, grupo B). Após três meses, sete animais foram sacrificados e os outros após seis meses. Em seguida, os espécimes foram preparados para análise histomorfométrica. Resultados: em ambos os grupos foram observados histologicamente um íntimo contato dos biomateriais com o tecido ósseo e a presença de novo tecido ósseo, biomateriais remanescentes, tecido conjuntivo e outros tecidos. Os valores da análise histomorfométrica, de formação do novo tecido ósseo para o grupo A e o grupo B, respectivamente, foram: 28,72% ± 11,81% e 30,69% ± 19,65% no período de três meses, e 23,37% ± 19,02% e 25,78% ± 22,75% no período de seis meses. Foram comparados todos os tecidos formados nos espécimes de três e seis meses entre os grupos A e B, e entre o mesmo grupo nos diferentes períodos de tempo. Não houve diferença estatisticamente signifi cante em nenhuma das comparações. Conclusão: baseado nos resultados, os biomateriais mostraram resultados histológicos e histomorfométricos semelhantes em alvéolos de extração de coelhos...

The aim of this study was to perform an histomorphometric evaluation of tissue formed using the rabbit extraction socket model, with application of two different biomaterials over two different post-surgical follow-up periods. Materials and Methods: fourteen male, New Zealand rabbits were used in this study. Two molars were extracted from the same side of the jaw on each rabbit, having one socket filled with bovine bone mineral (group A) and the other with a synthetic hydroxyapatite (group B). In each group, half of the animals were sacrificed at 3 and 6 months of healing. All samples were prepared for histomorphometric evaluation. Results: in both groups, direct contact of biomaterial with bone tissue was observed, and the presence of new bone, remaining biomaterials, fi brous tissue, and other tissues was demonstrated. Histomorphometric values for new bone tissue formation in groups A and B were 28.72% ± 11.81% and 30.69% ± 19.65%, respectively, at 3 months of healing and 23.37% ± 2.19% and 25.78% ± 22.75%, respectively, at 6 months of healing time. No statistically signifi cant differences were seen for intra and intergroup comparisons at three- and six-month healing periods. Conclusions: these results suggest that the two biomaterials used in this study have similar histologic and histomorphometric results using the rabbit extraction socket model...

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OBJECTIVE: USING BIOMECHANICAL STUDIES, THIS RESEARCH AIMS TO COMPARE HIP CAPSULORRHAPHY IN RABBITS, CARRIED OUT WITH TWO DIFFERENT TECHNIQUES: capsulorrhaphy with simple sutures and with anchors. METHOD: Thirteen New Zealand Albino (Oryctolaguscuniculus) male rabbits, twenty-six hip joints, were used. First, a pilot project was performed with three rabbits (six hip joints). This experiment consisted of ten rabbits divided into two groups: group 1 underwent capsulorrhaphy on both right and left hips with simple suture using polyglycolic acid absorbable thread, and group 2 underwent capsulorrhaphy with titanium anchors. After a four-week postoperative period, the animals were euthanized and the hip joints were frozen. On the same day of the biomechanical studies, after the hip joints were previously unfrozen, the following parameters were evaluated: rigidity, maximum force, maximum deformity and energy. RESULTS: There was no relevant statistical difference in rigidity, maximum force, maximum deformity and energy between the simple suture and anchor groups. CONCLUSION: Through biomechanical analyses, using parameters of rigidity, maximum force, maximum deformity and energy, it has been shown that capsulorrhaphy with simple suture and with anchors has similar results in rabbit hip joints. Level of Evidence II, Prospective Comparative Study .

RESUMO

OBJETIVO: Por meio de ensaios biomecânicos, comparar as capsulorrafias com sutura simples e com âncoras, em quadris de coelhos. MÉTODO: Foram utilizados 13 coelhos, 26 quadris, todos machos da raça Nova Zelândia albinos (Oryctolaguscuniculus). Inicialmente, realizamos um projeto piloto em três coelhos (seis quadris). Este experimento constou de 10 coelhos, divididos em 2 grupos: o Grupo 1 submetido à capsulorrafia (quadris direito e esquerdo) com sutura simples utilizando fio absorvível de ácido poliglicólico e o Grupo 2 submetido a capsulorrafia (quadris direito e esquerdo) com âncora de titânio. Após o período de quatro semanas de operados, todos animais foram submetidos à eutanásia e seus quadris congelados. Após um descongelamento prévio das peças, no mesmo dia das análises biomecânicas, foram avaliados os parâmetros de rigidez, força máxima, deformidade máxima e energia. RESULTADOS: Não houve diferença estatisticamente significante em relação à força no limite de proporcionalidade, rigidez e força máxima entre os grupos com sutura simples e com âncora. CONCLUSÃO: Por meio dos ensaios biomecânicos, tendo como parâmetro a rigidez, a força máxima, a deformidade máxima e a energia, ficou demonstrado que as capsulorrafias em quadris de coelhos com sutura simples e com âncora são semelhantes entre si. Nível de Evidência II, Estudo Prospectivo Comparativo.

OBJECTIVE: Using biomechanical studies, this research aims to compare hip capsulorrhaphy in rabbits, carried out with two different techniques: capsulorrhaphy with simple sutures and with anchors. METHOD: Thirteen New Zealand Albino (Oryctolaguscuniculus) male rabbits, twenty-six hip joints, were used. First, a pilot project was performed with three rabbits (six hip joints). This experiment consisted of ten rabbits divided into two groups: group 1 underwent capsulorrhaphy on both right and left hips with simple suture using polyglycolic acid absorbable thread, and group 2 underwent capsulorrhaphy with titanium anchors. After a four-week postoperative period, the animals were euthanized and the hip joints were frozen. On the same day of the biomechanical studies, after the hip joints were previously unfrozen, the following parameters were evaluated: rigidity, maximum force, maximum deformity and energy. RESULTS: There was no relevant statistical difference in rigidity, maximum force, maximum deformity and energy between the simple suture and anchor groups. CONCLUSION: Through biomechanical analyses, using parameters of rigidity, maximum force, maximum deformity and energy, it has been shown that capsulorrhaphy with simple suture and with anchors has similar results in rabbit hip joints. Level of Evidence II, Prospective Comparative Study.

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