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Understanding leaf generation dynamics, their seasonal changes, and their responses to nitrogen fertilization (NF) is key to improving pasture utilization efficiency. The objectives of this research were to determine structural and morphogenetic variables underlying changes in herbage mass on a set of Paspalum notatum genotypes. Ten P. notatum genotypes were evaluated in experimental plots following a completely randomized block design under a split-plot arrangement for two N-rates during four periods. Increased herbage mass (HM) after N-fertilization was explained by a higher tiller density (TD) (41.8%) and tiller weight (TW) (22.1%). The increment of TW after NF was due to the increase in leaf blade length (LBL) and width (LBW). During the flowering season, NF increases the reproductive tiller density by 262.5%. Seasonal variation in HM was mainly explained by changes in LBL that modified TW. Morphogenetic traits differed between genotypes of different growth habits; therefore, different management practices are suggested. The average increase in leaf elongation rate in response to NF was about 36.7%, generating longer leaves despite reductions in leaf elongation time (LET). The depletion in LBL and consequently in TW and HM during the autumn was attributed to the reduction in LET.

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Climate change is a major constraint on the sustainability of the humid tropics, maintaining ecosystem services, food production, and social functioning. Humid tropics play an essential role in C storage and greenhouse gas (GHG) emission reduction. Unfortunately, unplanned economic exploration, human occupation, and lack of knowledge of techniques to maintain ecosystem services negatively affect the humid tropics. In this study, we focused on the mechanisms of GHG emissions, C storage, and their mitigation strategies. This review indicated technologies that can be adopted by farmers in humid tropics to maintain or increase their capacity to store C stocks and reduce GHG emissions. The adoption of climate-smart agriculture technologies and the regulation of ecosystem services markets will accelerate the progress of preserving the humid tropics. Improved management practices, such as proper N fertilizer management and the introduction of N2-fixing legumes, can increase soil C sequestration, providing economic and environmental trade-offs associated with these management strategies. Public and private investments toward knowledge dissemination and technology adoption regarding GHG emissions reduction and soil C storage are needed to allow humid tropics to maintain their critical function of generating environmental and societal benefits.

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Vineyard soils normally do not provide the amount of nitrogen (N) necessary for red wine production. Traditionally, the N concentration in leaves guides the N fertilization of vineyards to reach high grape yields and chemical composition under the ceteris paribus assumption. Moreover, the carryover effects of nutrients and carbohydrates stored by perennials such as grapevines are neglected. Where a well-documented database is assembled, machine learning (ML) methods can account for key site-specific features and carryover effects, impacting the performance of grapevines. The aim of this study was to predict, using ML tools, N management from local features to reach high berry yield and quality in 'Alicante Bouschet' vineyards. The 5-year (2015-2019) fertilizer trial comprised six N doses (0-20-40-60-80-100 kg N ha-1) and three regimes of irrigation. Model features included N dosage, climatic indices, foliar N application, and stem diameter of the preceding season, all of which were indices of the carryover effects. Accuracy of ML models was the highest with a yield cutoff of 14 t ha-1 and a total anthocyanin content (TAC) of 3900 mg L-1. Regression models were more accurate for total soluble solids (TSS), total titratable acidity (TTA), pH, TAC, and total phenolic content (TPC) in the marketable grape yield. The tissue N ranges differed between high marketable yield and TAC, indicating a trade-off about 24 g N kg-1 in the diagnostic leaf. The N dosage predicted varied from 0 to 40 kg N ha-1 depending on target variable, this was calculated from local features and carryover effects but excluded climatic indices. The dataset can increase in size and diversity with the collaboration of growers, which can help to cross over the numerous combinations of features found in vineyards. This research contributes to the rational use of N fertilizers, but with the guarantee that obtaining high productivity must be with adequate composition.

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Snap bean is a vegetable crop presenting high requirements of mineral nutrients, such as nitrogen (N). However, studies on the response of this crop to N fertilizationare scarce, mainly with determinate growth habit genotypes, making it difficult to manage thisnutrient supply. The aim of this study was to evaluate the nitrogen nutrition and the productive performance of snap bean withdeterminate growth habit as a function of N sources and rates. The experiment was carried out inpots, under greenhouse conditions, inaEutrophicRed Oxisolwith 36.60 g dm-3of soil organic matter content.The cultivar ‘Macarrãorasteiro’TopSeedwas grownin a completely randomized design at factorial scheme 3x4, with four replications. It was studied three sources of N (urea, ammonium sulfate and sulfammo) and four rates (0, 40, 80 and 120 kg N ha-1), applied20% at sowing time, 40% at 12 days after emergence (DAE) and 40% at 20 DAE.It was evaluatedthe N content of the index leafandtheNaccumulationinshoots, the plant height at the endof the cycle and the number, total fresh mass, mean fresh mass and mean length of pods.The N content of the index leaf and N accumulation in shoots were linearly increased by the N rates, regardless of the N source, while the plant height and the pod’s characteristics werenotinfluenced by anyof the factors studied.

O feijão-vagem é uma hortaliça que apresenta elevada demanda por nutrientes minerais, dentre eles o nitrogênio (N). No entanto, são escassos os estudos de resposta da cultura à adubação nitrogenada, principalmente para cultivares de crescimento determinado, dificultando o manejo de fornecimento do nutriente. O objetivo deste trabalho foi avaliar a nutrição nitrogenada e o desempenho produtivo de feijão-vagem de crescimento determinado em função de fontes e doses de N. O experimento foi conduzido em vasos, sob condições de casa de vegetação, em Latossolo Vermelhoeutróficocom teor de matéria orgânica de 36,60 g dm-3. A variedade ‘Macarrão rasteiro’ TopSeedfoi cultivada em delineamento experimental inteiramente casualizado com esquema fatorial 3x4, com quatro repetições. Estudaram-se três fontes de N (ureia, sulfato de amônio e sulfammo) e quatro doses (0, 40, 80 e 120 kg N ha-1), parceladas 20% na semeadura, 40% aos 12 dias após a emergência (DAE) e 40% aos 20 DAE das plântulas. Foram avaliados o teor de N na folha-índice, o acúmulo de N na parte aérea, a altura das plantas ao final do ciclo, o número, a massa fresca total, a massa fresca médiae o comprimento médio das vagens. O teor foliar e o acúmulo de N na parte aérea das plantas foi aumentado linearmente com as doses, independente da fonte utilizada, enquanto a altura das plantas e as características relacionadas às vagens não foram influenciadas por nenhum dos fatores estudados.

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Snap bean is a vegetable crop presenting high requirements of mineral nutrients, such as nitrogen (N). However, studies on the response of this crop to N fertilizationare scarce, mainly with determinate growth habit genotypes, making it difficult to manage thisnutrient supply. The aim of this study was to evaluate the nitrogen nutrition and the productive performance of snap bean withdeterminate growth habit as a function of N sources and rates. The experiment was carried out inpots, under greenhouse conditions, inaEutrophicRed Oxisolwith 36.60 g dm-3of soil organic matter content.The cultivar ‘MacarrãorasteiroTopSeedwas grownin a completely randomized design at factorial scheme 3x4, with four replications. It was studied three sources of N (urea, ammonium sulfate and sulfammo) and four rates (0, 40, 80 and 120 kg N ha-1), applied20% at sowing time, 40% at 12 days after emergence (DAE) and 40% at 20 DAE.It was evaluatedthe N content of the index leafandtheNaccumulationinshoots, the plant height at the endof the cycle and the number, total fresh mass, mean fresh mass and mean length of pods.The N content of the index leaf and N accumulation in shoots were linearly increased by the N rates, regardless of the N source, while the plant height and the pods characteristics werenotinfluenced by anyof the factors studied.(AU)

O feijão-vagem é uma hortaliça que apresenta elevada demanda por nutrientes minerais, dentre eles o nitrogênio (N). No entanto, são escassos os estudos de resposta da cultura à adubação nitrogenada, principalmente para cultivares de crescimento determinado, dificultando o manejo de fornecimento do nutriente. O objetivo deste trabalho foi avaliar a nutrição nitrogenada e o desempenho produtivo de feijão-vagem de crescimento determinado em função de fontes e doses de N. O experimento foi conduzido em vasos, sob condições de casa de vegetação, em Latossolo Vermelhoeutróficocom teor de matéria orgânica de 36,60 g dm-3. A variedade ‘Macarrão rasteiro TopSeedfoi cultivada em delineamento experimental inteiramente casualizado com esquema fatorial 3x4, com quatro repetições. Estudaram-se três fontes de N (ureia, sulfato de amônio e sulfammo) e quatro doses (0, 40, 80 e 120 kg N ha-1), parceladas 20% na semeadura, 40% aos 12 dias após a emergência (DAE) e 40% aos 20 DAE das plântulas. Foram avaliados o teor de N na folha-índice, o acúmulo de N na parte aérea, a altura das plantas ao final do ciclo, o número, a massa fresca total, a massa fresca médiae o comprimento médio das vagens. O teor foliar e o acúmulo de N na parte aérea das plantas foi aumentado linearmente com as doses, independente da fonte utilizada, enquanto a altura das plantas e as características relacionadas às vagens não foram influenciadas por nenhum dos fatores estudados.(AU)

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The application of new agricultural technologies to attain sustainable production systems is necessary. The use of plant growth-promoting bacteria to improve plant growth and health has been studied for decades. This work aimed to isolate diazotrophic endophytic bacteria associated with sweet sorghum plants and study the interaction of their inoculation in combination with chemical N-fertilization on different sorghum cultivars. A bacterial collection of 181 isolates was constructed and characterized in vitro and in vivo. From that, the strains Enterobacter sp. UYSB89 and Kosakonia sp. UYSB139 were nifH+, produce IAA, defined as true endophytes and able to promote growth of two sweet sorghum under greenhouse conditions. The evaluated cultivars responded differentially to bacterial inoculation, the nitrogen fertilization doses and their interaction. Thus, plant growth is a multifactorial consequence of the interrelation between crop practices and the plant genotypes. This knowledge is a valuable factor in terms of understanding plant-bacteria endophyte interactions to preserve environmental sustainability during the implementation of agronomic practices.

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Foliar fungal diseases may cause important losses on yield and quality of wheat (Triticum aestivum L.). They may impact crop growth rate differently, modifying nitrogen (N) dynamics and carbohydrate accumulation in the grain. The relationship between N and carbohydrates accumulation determines the grain protein concentration, which impacts the gluten concentration and rheological properties of the wheat flour. In addition, types of fungicides and N fertilization can influence the intensity of foliar diseases and have an effect on the milling and end-use quality, depending on the bread-making aptitude of the genotypes, the nutritional habit of the pathogen involved, the amount and time of infection, environmental factors, and interactions between these factors. In that way, N fertilization may modify the severity of the diseases according to the nutritional habit of the pathogen involved. Some fungicides, such as strobilurins and carboxamides, produce high levels of disease control and prolong the healthy leaf area duration, which translates into important yield responses, potentially compromising the grain protein concentration by additional carbohydrate production, with consequences in the bread-making quality. Furthermore, infections caused by biotrophic pathogens can be more damaging to N deposition than to dry matter accumulation, whereas the reverse has been generally true for diseases caused by necrotrophic pathogens. The time of infection could also affect yield components and N dynamics differentially. Early epidemics may reduce the number of grains per area and the N remobilization, whereas late epidemics may affect the thousand kernel weight and mainly the N absorption post-flowering. A review updating findings of the effects of infections caused by foliar fungal pathogens of different nutritional habits and the incidence of several factors modifying these effects on the above-ground biomass generation, N dynamics, protein and gluten concentration, milling, rheological properties, loaf volume, and other quality-related traits is summarized. Three main pathogens in particular, for which recent information is available, were taken as representative of biotrophic (Puccinia triticina), necrotrophic (Pyrenophora tritici-repentis), and hemibiotrophic (Zymoseptoria tritici) nutritional habit, and some general models of their effects are proposed. New challenges for researchers to minimize the impact of foliar diseases on end-use quality are also discussed.

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Soybean [Glycine max (L.) Merr.] grain yield is closely associated with the level of optimal nitrogen (N) supply, especially during the reproductive stages. Foliar fertilization with low rates of N have been considered as a strategy for furnishing additional N and enhancing grain yields. Field studies using 15N tracer were conducted over two growing seasons to investigate the impact of foliar N fertilization on grain yield, plant N content, the amount of N derived from fertilizer (NDFF) and N recovery efficiency (NRE). Four foliar N rates (0, 1300, 2600 and 3900 g ha−1) were supplied by two equal split applications at the R1 and R3 stages. Foliar N fertilization of soybean canopies did not affect grain yield, grain N content, shoot N content nor plant N content. Total NDFF was increased from 0.7 to 2.0 kg ha−1 across the N rates. Nonetheless, NRE was unaffected by foliar N fertilization, which averaged 53 %. Soybean plants allocated the same amount of N fertilizer to both grains and shoots. No significant effects of low rate foliar N fertilization were registered on soybean grain yield nor plant N content, despite considerable N fertilizer recovery by plant organs.(AU)

RESUMO

Soybean [Glycine max (L.) Merr.] grain yield is closely associated with the level of optimal nitrogen (N) supply, especially during the reproductive stages. Foliar fertilization with low rates of N have been considered as a strategy for furnishing additional N and enhancing grain yields. Field studies using 15N tracer were conducted over two growing seasons to investigate the impact of foliar N fertilization on grain yield, plant N content, the amount of N derived from fertilizer (NDFF) and N recovery efficiency (NRE). Four foliar N rates (0, 1300, 2600 and 3900 g ha−1) were supplied by two equal split applications at the R1 and R3 stages. Foliar N fertilization of soybean canopies did not affect grain yield, grain N content, shoot N content nor plant N content. Total NDFF was increased from 0.7 to 2.0 kg ha−1 across the N rates. Nonetheless, NRE was unaffected by foliar N fertilization, which averaged 53 %. Soybean plants allocated the same amount of N fertilizer to both grains and shoots. No significant effects of low rate foliar N fertilization were registered on soybean grain yield nor plant N content, despite considerable N fertilizer recovery by plant organs.

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Low efficiency of nitrogen from fertilizers is a major concern worldwide, threatening the sustainability of sugarcane production. The N use efficiency (NUE) by sugarcane can be improved by adopting better fertilizing management practices, reducing environmental impacts. This work evaluated the effects of varying N rates and time of application on stalks and sugar yield in ratoon harvested early in the crop season. The experimental design was a randomized block in a 2 × 4 factorial design and a control (no N) with five replications, including two application times (45 or 90 DAH days after harvest) and four N rates (50, 100, 150, or 200 kg N ha−1). The time of N fertilizer application promoted differences in stalk yield, as the cumulative yield of two harvests was increased by 8 % (15 Mg ha−1) at 45 DAH when compared to the application at 90 DAH. The application performed at 45 DAH augmented sugar yield by 10 % (2.8 Mg ha−1 of sugar) in relation to 90 DAH. The N rates that promoted the highest sugarcane yield were, respectively, 122 and 144 kg N ha−1 in the first and second crop cycles. The average economical rates obtained for the first and second agricultural cycles were, respectively, 104 and 127 kg N ha−1, demonstrating that the gains by applying high amounts of fertilizers (rates above 150 kg N ha−1) may not cover the investment.(AU)

RESUMO

Low efficiency of nitrogen from fertilizers is a major concern worldwide, threatening the sustainability of sugarcane production. The N use efficiency (NUE) by sugarcane can be improved by adopting better fertilizing management practices, reducing environmental impacts. This work evaluated the effects of varying N rates and time of application on stalks and sugar yield in ratoon harvested early in the crop season. The experimental design was a randomized block in a 2 × 4 factorial design and a control (no N) with five replications, including two application times (45 or 90 DAH – days after harvest) and four N rates (50, 100, 150, or 200 kg N ha−1). The time of N fertilizer application promoted differences in stalk yield, as the cumulative yield of two harvests was increased by 8 % (15 Mg ha−1) at 45 DAH when compared to the application at 90 DAH. The application performed at 45 DAH augmented sugar yield by 10 % (2.8 Mg ha−1 of sugar) in relation to 90 DAH. The N rates that promoted the highest sugarcane yield were, respectively, 122 and 144 kg N ha−1 in the first and second crop cycles. The average economical rates obtained for the first and second agricultural cycles were, respectively, 104 and 127 kg N ha−1, demonstrating that the gains by applying high amounts of fertilizers (rates above 150 kg N ha−1) may not cover the investment.

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Factors regulating fine-root growth are poorly understood, particularly in fruit tree species. In this context, the effects of N addition on the temporal and spatial distribution of fine-root growth and on the fine-root turnover were assessed in irrigated sweet cherry trees. The influence of other exogenous and endogenous factors was also examined. The rhizotron technique was used to measure the length-based fine-root growth in trees fertilized at two N rates (0 and 60 kg ha(-1)), and the above-ground growth, leaf net assimilation, and air and soil variables were simultaneously monitored. N fertilization exerted a basal effect throughout the season, changing the magnitude, temporal patterns and spatial distribution of fine-root production and mortality. Specifically, N addition enhanced the total fine-root production by increasing rates and extending the production period. On average, N-fertilized trees had a length-based production that was 110-180% higher than in control trees, depending on growing season. Mortality was proportional to production, but turnover rates were inconsistently affected. Root production and mortality was homogeneously distributed in the soil profile of N-fertilized trees while control trees had 70-80% of the total fine-root production and mortality concentrated below 50 cm depth. Root mortality rates were associated with soil temperature and water content. In contrast, root production rates were primarily under endogenous control, specifically through source-sink relationships, which in turn were affected by N supply through changes in leaf photosynthetic level. Therefore, exogenous and endogenous factors interacted to control the fine-root dynamics of irrigated sweet cherry trees.

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O nitrogênio (N), além de ser um dos macronutrientes mais absorvidos pelas plantas, é o que mais limita a produção de girassol. O objetivo deste trabalho foi avaliar os teores de nutrientes nas folhas, nos grãos e a exportação de nutrientes em função de níveis de N na cultura do girassol. Foi adotado o delineamento experimental em blocos casualizados constituído por cinco tratamentos e quatro repetições: T1 - 50 kg ha-1 de N dividido em 10, 20 e 20 kg ha-1; T2 - 70 kg ha-1 de N dividido em 10, 30 e 30 kg ha-1; T3 - 90 kg ha-1 de N dividido em 10, 40 e 40 kg ha-1; T4 - 110 kg ha-1 de N dividido em 10, 50 e 50 kg ha-1; T5 - 130 kg ha-1 de N dividido em 10, 60 e 60 kg ha-1. Essas aplicações parceladas foram efetuadas quando na semeadura e em cobertura aos 34 e 48 dias após a semeadura. Os parâmetros avaliados foram teores de nutrientes nas folhas, teores de nutrientes nos grãos e a exportação de nutrientes. Nas condições experimentais verificou-se que os teores foliares adequados de N foram adquiridos na dose acima de 110 kg ha-1 de N. Com o aumento da quantidade de N fornecido na cultura do girassol foi exportado mais este elemento, porém não na mesma proporção de aumento da dose. A menor dose de N promoveu os menores índices de exportação de P, K, Mg, S, B, Cu, Mn e Zn, enquanto que o aumento da dose deste nutriente diminuiu a exportação de Ca.

Nitrogen (N), besides being one of the macro more absorbed by plants, is the that most limits the production of sunflower. The aim of this study was to evaluate the nutrient content in leaves, grains and nutrient export due to nitrogen levels in sunflower cultivation. A randomized experimental block design consisting of five treatments and four replications: T1 - 50 kg N ha-1 divided by 10, 20 and 20 kg ha-1, T2 - 70 kg N ha-1 divided by 10, 30 and 30 kg ha-1 ; T3 - 90 kg N ha-1 divided by 10, 40 and 40 kg ha-1 , T4 - 110 kg N ha-1 divided by 10, 50 and 50 kg ha-1 , T5 - 130 kg N ha- 1 divided by 10, 60 and 60 kg ha-1 . These applications were made in installments when sowing and 34 and 48 days after sowing. The parameters were evaluated in leaf nutrient content, nutrient content in grains and nutrient export. Under the experimental conditions it was found that the optimum foliar N were purchased at a dosage above 110 kg ha-1 N. With the increasing amount of N supplied in the sunflower crop was exported over this element, but not at the same rate of increase in dosage. The lowest dose of N the lowest rates of export of P, K, Mg, S, B, Cu, Mn and Zn, while increasing the dose of this nutrient decreased the export of Ca.

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Response of wheat to Azospirillum brasilense Sp-248 inoculation with different N-fertilizer levels using seawater irrigation was investigated. All inoculated treatments increased plant height, shoot and root dry weight, and tiller number in compared with uninoculated treatments. Yield parameters measured were also increased due to the inoculation. In terms of the effect of saline irrigation, there were no significant differences in growth and yield parameters in plants treated with tap water and others irrigated with 8.0% seawater concentration. This would indicate a relatively high tolerance of A. brasilense to saline irrigation and its ability to reduce the deleterious effects of saline on growth by increasing the plant's adaptation. However, increasing the seawater concentration in the irrigation water to 16.0% significantly decreased all tested parameters. Inoculation treatments generally increased NPKCa contents and decreased sodium ratio of the grains in compared with the uninoculated treatments. Overall results clearly revealed that the Azospirillum inoculation saved about 20 units of N-fertilizer and that saving was made economically feasible by decreasing the chemical fertilizers needed, improving the nitrogen content and counteracting the effects of salinity.