RESUMO

Abstract The prevalence of gingivitis is substantial within the general population, necessitating rigorous oral hygiene maintenance. Objective This study assessed a Garcinia indica (GI) fruit extract-based mouthrinse, comparing it to a 0.1% turmeric mouthrinse and a 0.2% Chlorhexidine (CHX) mouthrinse. The evaluation encompassed substantivity, staining potential, antimicrobial efficacy and cytocompatibility. Methodology The study employed 182 tooth sections. For antimicrobial analysis, 64 extracted human teeth coated with a polymicrobial biofilm were divided into four groups, each receiving an experimental mouthrinse or serving as a control group with distilled water. Microbial reduction was assessed through colony forming units (CFU). Substantivity was evaluated on 54 human tooth sections using a UV spectrophotometer, while staining potential was examined on 64 tooth sections. Cytocompatibility was tested using colorimetric assay to determine non-toxic levels of 0.2% GI fruit extract, 0.1% Turmeric, and 0.2% CHX. Results Data were analysed with one-way ANOVA (α=0.05). Cell viability was highly significant (p<0.001) in the 0.2% GI group (64.1±0.29) compared to 0.1% Turmeric (40.2±0.34) and 0.2% CHX (10.95±1.40). For antimicrobial activity, both 0.2% GI (20.18±4.81) and 0.2% CHX (28.22±5.41) exhibited no significant difference (P>0.05) at end of 12 hours. However, 0.1% Turmeric showed minimal CFU reduction (P<0.001). Substantivity results at 360 minutes indicated statistically significant higher mean release rate in 0.1%Turmeric (12.47±5.84 ) when compared to 0.2% GI (5.02±3.04) and 0.2% CHX (4.13±2.25) (p<0.001). The overall discoloration changes (∆E) were more prominent in the 0.2% CHX group (18.65±8.3) compared to 0.2% GI (7.61±2.4) and 0.1% Turmeric (7.32±4.9) (P<0.001). Conclusion This study supports 0.2% GI and 0.1% Turmeric mouth rinses as potential natural alternatives to chemical mouth rinses. These findings highlight viability of these natural supplements in oral healthcare.

RESUMO

OBJECTIVE: To investigate the effect of fluoride-containing whitening products on sound enamel and on artificial caries lesions during a cariogenic challenge. MATERIALS AND METHODS: Bovine enamel specimens (n = 120) with three areas [non-treated sound enamel (NSE), treated sound enamel (TSE), and treated artificial caries lesion (TACL)] were randomly assigned to the four groups: whitening mouthrinse (WM: 2.5% hydrogen peroxide-100 ppm F-), placebo mouthrinse (PM: 0% hydrogen peroxide-100 ppm F-), whitening gel (WG: 10% carbamide peroxide-1130 ppm F-), and deionized water (negative control; NC). The treatments (2 min for WM, PM, and NC, and 2 h for WG) were carried out during a 28-day pH-cycling model (6 × 60 min demineralization/day). Relative surface reflection intensity (rSRI) and transversal microradiography (TMR) analyses were performed. Fluoride uptake (surface and subsurface) was measured in additional enamel specimens. RESULTS: For TSE, a higher value of rSRI was observed in WM (89.99% ± 6.94), and a greater decrease in rSRI was observed for WG and NC, and no sign of mineral loss was verified for all groups (p > 0.05). For TACL, rSRI significantly decreased after pH-cycling for all experimental groups with no difference between them (p < 0.05). Higher amounts of fluoride were found in WG. WG and WM exhibited intermediate values of mineral loss, similar to PM. CONCLUSIONS: The whitening products did not potentialize the enamel demineralization under a severe cariogenic challenge, and they did not exacerbate mineral loss of the artificial caries lesions. CLINICAL RELEVANCE: Low concentrated hydrogen peroxide whitening gel and mouthrinse containing fluoride do not intensify the progression of caries lesions.

Assuntos

Cárie Dentária , Desmineralização do Dente , Animais , Bovinos , Cariostáticos/farmacologia , Cariostáticos/uso terapêutico , Cárie Dentária/patologia , Suscetibilidade à Cárie Dentária , Esmalte Dentário , Fluoretos/farmacologia , Peróxido de Hidrogênio/farmacologia , Concentração de Íons de Hidrogênio , Minerais/farmacologia , Antissépticos Bucais/farmacologia , Desmineralização do Dente/patologia , Remineralização Dentária

RESUMO

ABSTRACT: Background: Recurrent aphthous stomatitis (RAS) is the most common oral ulceration. Its prevalence in the general population varies between 5% and 60%, and during the acute period, it causes pain and interferes with basic activities, such as eating, swallowing and talking. Dentoxol® is a medical mouthrinse that cleans, moisturizes and lubricates the mouth, mechanically stimulating local epithelial regeneration. The aim of this study was to evaluate the efficacy of Dentoxol® in improving the general state of patient with minor RAS using two different treatment schemes. Material and methods: Thirty-nine patients with RAS were recruited in a prospective observational pilot study. Two dosing regimens, 5 ml of Dentoxol® twice daily and 5 ml of Dentoxol® three times daily were evaluated. Results: Efficacy to improve the general state was significant superior in "Three time daily" group compared with "twice daily" at 72 h (66% vs 33% respectively). No pain was reported in approximately 90% of cases at 96 h of use in both group without significant differences between the groups in any evaluation time-point. Conclusion: Despite the limitations of these preliminary data, Dentoxol® shows promising beneficial properties for the management of minor RAS.

Assuntos

Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Estomatite Aftosa/tratamento farmacológico , Antissépticos Bucais/uso terapêutico , Recidiva , Projetos Piloto , Estudos Prospectivos , Úlceras Orais/tratamento farmacológico

RESUMO

The aim of this study was to compare the antibacterial effect of eight different commercial (MWs) on two Streptococcus mutans (SM) strains by using the agar well diffusion method. Eight commercial MWs were selected, all of them combined several ingredients in different concentrations, the main active ingredients were: Chlorhexidine gluconate, cetylpyridinium chloride, sodium fluoride, zinc lactate, vitamin B5 and super-oxidized water. The SM strains were extracted from Cultiloops® and incubated for 24 hours at 37 °C. The antimicrobial activity was evaluated using the agar well diffusion method. The inhibition zones were measured using an electronic digital caliper. The R© software was used to perform the statistical analysis using Kruskall-Wallis test and Dunn's multiple comparisons test. Seven commercial formulas demonstrated inhibitory effect over both SM strains. Only the MW containing super-oxidized water did not exhibit antibacterial activity. Higher inhibitory effect was observed in the chlorhexidine gluconate formula (27.38 ± 0.98 mm and 31.52 ± 0.64 mm). No statistically significant differences were observed when comparing formulas containing chlorhexidine gluconate in combination with other active ingredients. Seven MWs showed antibacterial activity except super-oxidized water formula. MWs containing chlorhexidine gluconate demonstrated the best effect against SM. However, no statistically significant differences were found when comparing formulas using exclusively chlorhexidine gluconate or combined with other antiseptics. Future research must be performed, focused on developing new MWs with similar antibacterial effects to chlorhexidine, but free of side effects, particularly in long-term treatments.

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El objetivo de este estudio fue comparar el efecto antibacteriano de ocho colutorios comerciales en la proliferación de dos cepas de Streptococcus mutans (SM) mediante el método de difusión de pozos de agar. Se seleccionaron ocho colutorios comerciales, todos ellos combinados con varios ingredientes en diferentes concentraciones, los principales ingredientes activos fueron: gluconato de clorhexidina, cloruro de cetilpiridinio, fluoruro de sodio, lactato de zinc, vitamina B5 y agua superoxidada. Las cepas SM se extrajeron de Cultiloops® y se incubaron durante 24 horas a 37 °C. La actividad antimicrobiana se evaluó mediante el método de difusión de placa de agar. Las zonas de inhibición se midieron utilizando un calibre digital electrónico. Se utilizó el software R © para realizar el análisis estadístico mediante la prueba de Kruskall-Wallis y la prueba de comparaciones múltiples de Dunn. Siete fórmulas comerciales demostraron efecto inhibidor sobre ambas cepas SM. Solo el colutorio que contenía agua superoxidada no mostró actividad antibacteriana. Se observó un mayor efecto inhibidor en las fórmulas congluconato de clorhexidina (27,38 ± 0,98 mm y 31,52 ± 0,64 mm). No se observaron diferencias estadísticamente significativas al comparar fórmulas que contienen gluconato de clorhexidina en combinación con otros ingredientes activos. Siete MW mostraron actividad antibacteriana excepto la fórmula de agua superoxidada. Los colutorios que contienen gluconato de clorhexidina mostraron el mejor efecto contra SM. Sin embargo, no se encontraron diferencias estadísticamente significativas al comparar fórmulas que combinaron con otros principios activos. Se deben realizar investigaciones, enfocadas en el desarrollo de nuevos colutorios con efectos antibacterianos similares a la clorhexidina, pero libres de efectos secundarios, particularmente en tratamientos a largo plazo.

RESUMO

To evaluate the effect of herbal mouthrinses as an adjuvant to oral hygiene on dental plaque and gingival inflammation in subjects with gingivitis. Searches were conducted in the PubMed/MEDLINE, Cochrane-CENTRAL, EMBASE, Web of Science, LILACS/BIREME, Clinical Trials Registry and grey literature for Randomised Clinical Trials (RCTs) published up to April 2018 without language restrictions. From 4,013 paper found, 20 studies met the eligibility criteria and were included. The herbal mouthrinses achieved significant reductions in dental plaque and gingival inflammation compared to placebo rinses. Five herbal products (Camelia sinensis, Azadirachta indica, Anacardium occidentale Linn, Schinus terebinthifolius and Curcuma longa) showed better results than chlorhexidine in dental plaque and gingival inflammation reductions. However, the unclear risk of bias of most included RCTs precludes definitive conclusions. Therefore, it is necessary to improve the design of future RCT in other reduced potential bias that may affect the degree of precision of treatment outcomes in order to evaluate the effect size and clinical relevance of herbal mouthrinses.

Assuntos

Placa Dentária , Gengivite , Clorexidina/uso terapêutico , Placa Dentária/tratamento farmacológico , Placa Dentária/prevenção & controle , Gengivite/tratamento farmacológico , Gengivite/prevenção & controle , Humanos , Inflamação , Antissépticos Bucais/uso terapêutico

RESUMO

OBJECTIVES: 1. To evaluate the use of fluoridated mouthrinses before or after toothbrushing on erosive tooth wear. 2. To compare the anti-erosive effect of the combination toothpaste and mouthrinse containing fluoride, with or without stannous chloride. DESIGN: Enamel and dentin specimens were randomly distributed into groups (n = 10 of each substrate/group): B-brushing, B + R-brushing + rinsing, and R + B-rinsing + brushing. The treatments were performed using a fluoride toothpaste (BF: 1400 ppm fluoride, as amino fluoride-AmF) combined or not with a fluoride mouthrinse (RF: 250 ppm fluoride, as AmF and sodium fluoride-NaF) or fluoride and stannous toothpaste (BF+Sn: 1400 ppm fluoride, as AmF and NaF, 3500 ppm stannous, as stannous chloride-SnCl2 and 0.5% chitosan) combined or not with fluoride and stannous mouthrinse (RF+Sn: 500 ppm fluoride, as AmF and NaF, 800 ppm stannous, as SnCl2). As control, brushing was performed with artificial saliva (BC). Specimens were submitted to a 5-day erosive-abrasive cycling model. Treatments were performed twice daily. Surface loss (SL) was determined by optical profilometry. Data were analyzed by ANOVA and Games-Howell tests (α = 0.05). RESULTS: For enamel, RF+BF and RF+Sn+BF+Sn presented significantly lower SL than the control, with RF+BF being significantly lower than RF+Sn+BF+Sn. For dentin, BC had the lowest SL, not differing from BF+Sn+RF+Sn, RF+Sn+BF+Sn and BF. Groups RF+BF and BF+RF showed highest SL, not differing from BF+Sn and BF+Sn. CONCLUSIONS: For enamel, the use of a mouthrinse before brushing was able to reduce erosive wear for both fluoride and stannous products. For dentin, the use of stannous-containing products, irrespective of the order of application, presented superior effects.

Assuntos

Fluoretos , Abrasão Dentária , Erosão Dentária , Desgaste dos Dentes , Escovação Dentária , Humanos , Fluoreto de Sódio , Cremes Dentais

RESUMO

RESUMEN: El objetivo de este estudio fue evaluar el efecto de un enjuague oral de extracto acuoso de Rosmarinus Officinalis (romero) sobre la cicatrización de heridas de mucosa oral en sujetos sanos. Veintiocho estudiantes, de sexo masculino, edad promedio de 22 años, fueron reclutados para un estudio de doble ciego en la Facultad de Odontología de la Universidad de Concepción, Chile. Se creó una herida estándar de 3 mm de diámetro en la mucosa palatina, que fue fotografiada durante un período de 21 días para determinar su tamaño por fotoplanimetría. Los voluntarios recibieron enjuagues orales para ser utilizados a diario desde el día de creación de la herida, 3 veces al día, y fueron divididos en grupo control (enjuague placebo) y grupo experimental (enjuague de extracto de romero). El extracto acuoso de Romero fue analizado por HPLC, mostrando un alto contenido de carnosol (83 %) y ácido rosmarínico (65 %). Se observó una disminución significativa en el tamaño de la herida del grupo tratado con enjuague de romero comparado con el tratado con enjuague placebo al día 3 de cicatrización (20 %, p=0,046, Mann-Whitney). Posteriormente no se encontraron diferencias, completándose la cicatrización antes del día 21 para ambos grupos. Los resultados sugieren que el enjuagatorio de extracto acuoso de romero favorece la cicatrización temprana de las heridas, lo que puede deberse a su alto contenido de carnosol y ácido rosmarínico que han demostrado estimular la cicatrización y poseer actividad antimicrobiana.

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ABSTRACT: The objective of the study was to evaluate the effect of a mouthrinse containing an aqueous extract of Rosmarinus officinalis (rosemary) on healing wounds of the oral mucosa in healthy subjects. Twenty Eight healthy, non-smoking male students, mean age 22 years, were recruited for a double-blind study at the School of Dentistry of the University of Concepción, Chile. A standard 3mm wound was created on the palate with a punch biopsy. The wounds were photographed to assess healing for 21 days. The subjects were instructed to use a mouthrinse 3 times daily for 21 days, starting the day of wounding, and were divided into a control group (placebo) and an experimental group (rosemary extract). The aqueous rosemary extract used for the mouthrinse had a high content of carnosol (83 %) and rosmarinic acid (65 %) as determined by HPLC. We observed a 20 % reduction in wound size by day 3 in the rosemary mouthrinse group as compared to the placebo mouthrinse group (p=0.04, Mann Whitney). Thereafter, no significant differences in wound closure were observed. Healing was completed by day 21 in both groups. The results suggest that the mouthrinse containing the aqueous rosemary extract stimulates early healing of mucosal wounds. This could be due to the high content of carnosol and rosmarinic acid, which are known for their healing and antimicrobial properties.

Assuntos

Humanos , Masculino , Adulto Jovem , Rosmarinus/química , Mucosa Bucal/lesões , Cicatrização , Chile , Estatísticas não Paramétricas , Antissépticos Bucais/uso terapêutico

RESUMO

RESUMEN: Numerosos estudios confirman la efectividad de los enjuagatorios orales sobre la viabilidad de los microorganismos que producen gingivitis y halitosis, pero poco se conoce sobre la influencia de los mismos en el medio ambiente oral. El objetivo del siguiente trabajo fue analizar In vivo e In vitro el efecto de enjuagatorios orales sobre la saliva total no estimulada. Se trabajó con saliva de individuos sanos. Para el estudio in vivo se recogieron las muestras antes y después del enjuague oral a diferentes tiempos (1, 5, 10, 15, 30, 45 y 60 minutos). Para el ensayo in vitro, se incubó la saliva con igual volumen de la solución enjuagatoria a 37 ºC con agitación a diferentes tiempos (1, 5, 10 y 15 minutos). Se determinó pH inmediatamente recogidas las muestras. Posteriormente fueron centrifugadas y determinados flujo salival y proteínas totales. La separación de proteínas por electroforesis en SDS-PAGE se realizó solo en el ensayo in vivo. Los resultados mostraron que los enjuagatorios fluorurados poco alteran la fisiología oral a través de flujo salival, pH y proteínas totales. La combinación fluoruro de sodio/xilitol produjo mayor estimulación del flujo salival. La mezcla de aceites esenciales provocó un incremento del flujo salival y de pH, redujo el contenido de proteínas totales, evidenciando por SDS-PAGE que las comprometidas fueron particularmente las de mediano y bajo peso molecular. Clorhexidina debido a su elevada sustantividad, incrementó significativamente flujo salival y pH in vivo. In vitro, fuera del medioambiente oral, los enjuagatorios estudiados ejercieron un efecto similar sobre proteínas totales. Los enjuagatorios de uso frecuente alteraron parámetros salivales, por lo que podría estudiarse la acción que ejercen sobre otros componentes de la saliva con actividad biológica importante en cavidad oral.

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ABSTRACT: Numerous studies confirm the effectiveness of mouthwashes on the viability of microorganisms that produce gingivitis and halitosis, but little is known about their influence on the oral environment. The objective of the following work was to analyze In vivo and In vitro the effect of mouthwashes on total non-stimulated saliva. We worked with saliva from healthy individuals. For In vivo study, samples were collected before and after oral rinsing at different times (1, 5, 10, 15, 30, 45 and 60 minutes). For the In vitro assay, the saliva was incubated with equal volume of the rinse solution at 37 ° C with shaking at different times (1, 5, 10 and 15 minutes). PH was determined immediately collected samples. Subsequently they were centrifuged and determined salivary flow and total proteins. Separation of proteins by SDS-PAGE electrophoresis was performed only in the In vivo assay. The results showed that fluoridated rinses hardly alter oral physiology through salivary flow, pH and total proteins. The combination of sodium fluoride / xylitol produced greater stimulation of salivary flow. The mixture of essential oils caused an increase in salivary flow and pH, reduced the total protein content, evidencing by SDS-PAGE that those involved were particularly those of medium and low molecular weight. Chlorhexidine due to its high substantivity, significantly increased salivary flow and pH In vivo. In vitro, outside the oral environment, the rinses studied had a similar effect on total proteins. Rinses used frequently altered salivary parameters, so that the action they exert on other components of saliva with important biological activity in the oral cavity could be studied.

Assuntos

Humanos , Adulto , Pessoa de Meia-Idade , Saliva/efeitos dos fármacos , Gengivite , Antissépticos Bucais/farmacologia , Técnicas In Vitro , Halitose

RESUMO

Introduction: mechanical hygiene is still the best method forthe maintenance of oral health and prevention of biofilm formation. However, mouthrinses have been increasingly used as an adjunct support especially for post-operatory conditions or when periodontal conditions require. In this way, a number of mouthrinses are available, presenting different active ingredients with predominant antimicrobial action. Objectives: the aim of the present study was to evaluate the topical action of a green-tea extract-based mouthwash on gingival tissue. Materials and Methods: a longitudinal double-blind comparison was performed with two groups of patients clinically evaluated and diagnosed as suffering from gingivitis (n= 40). The patients were divided into two groups, according to the mouthrinse used: GT - green tea (concentration of 20mg/ml), and P - placebo, 0.9% saline solution. Clinical examination of the mucosa, tooth staining, plaque and gingival indices were peformed before and 15 days after the continuous use of the products twice a day. Results: no mucosal or tasting alterations were found, neither tooth staining regardless the mouthrinse used. Statistical assessment did not detect differences on gingival indexes between the groups before and after mouthrinses use; however, plaque indexes were significant lower in patients of GT group, compared to the P group. Conclusions: the use of phytotherapic products with active ingredients should be investigated for biofilm control, responsible for the onset and progression of periodontal disease, as well as other various oral pathologies. Considering the period of evaluation pro-posed in this study, relevant decrease in initial phase of plaque for-mation could be observed with the green tea mouthrinse. Further long-term evaluation studies should be carried-out in order to eluci-date its continuous effects on oral structures.

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Introdução: higiene mecânica ainda é o melhor método para a manutenção da saúde bucal e prevenção da formação de biofilmes. No entanto, enxagues têm sido cada vez mais usado como um suporte auxiliar especialmente para as condições pós-operatórias ou quando as condições periodontais exigem. Desta forma, um número de bochechos estão disponíveis, apresentando diferentes ingredientes ativos com predominante ação antimicrobiana. Objetivos: o objetivo do presente estudo foi avaliar a ação tópica de um bochecho à base de extrato de chá verde no tecido gengival. Materiais e Métodos: a comparação longitudinal, duplo-cego, foi realizada com dois grupos de pacientes clinicamente avaliados e diagnosticados como portadores de gengivite (n = 40). Os pacientes foram divididos em dois grupos, de acordo com o enxaguatório oral usado: CV - chá verde (concentração de 20 mg / ml), e P - placebo, solução salina a 0,9%. O exame clínico da mucosa, coloração dos dentes, índice de placa e índice gengival foram realizados antes e 15 dias após o uso contínuo dos produtos duas vezes por dia. Resultados: nenhuma alteração na mucosa ou de degustação foi encontrada, assim como não foi identificado modificação na coloração do dente. A Avaliação estatística não detectou diferenças no índice gengival entre os grupos antes e depois de usar enxaguatórios. No entanto, índice de placa inferior foi menor de forma significativa nos pacientes do grupo CV, em comparação com o grupo P. Conclusões: o uso de produtos fitoterápicos com ingredientes ativos deve ser investigado para o controle de biofilme, responsável pelo aparecimento e progressão da doença periodontal. Considerando o período de avaliação proposto, a redução correspondente na fase inicial da formação de placa pôde ser observada com o enxaguatório à base de chá verde. Além disso, estudos de avaliação de longo prazo devem ser realizados, a fim de elucidar seu efeito contínuo em longo prazo sobre as estruturas orais.

Assuntos

Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Gengivite/tratamento farmacológico , Antissépticos Bucais/análise , Antissépticos Bucais/uso terapêutico , Extratos Vegetais/uso terapêutico , Método Duplo-Cego , Camellia sinensis/efeitos adversos

RESUMO

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...

Assuntos

Animais , Antissépticos Bucais/administração & dosagem , Cavalos , Lippia , Mucosa Bucal/lesões , Óleos Voláteis/administração & dosagem , Doenças Periodontais/terapia , Doenças Periodontais/veterinária

RESUMO

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...(AU)

Assuntos

Animais , Cavalos , Lippia , Óleos Voláteis/administração & dosagem , Antissépticos Bucais/administração & dosagem , Mucosa Bucal/lesões , Doenças Periodontais/terapia , Doenças Periodontais/veterinária

RESUMO

This study sought to evaluate the clinical effect of a mouthrinse containing 0.3% chlorine dioxide (ClO2) in reducing oral volatile sulfur compounds (VSC). Halitosis was induced by L-cysteine in 11 volunteers, and 4 solutions were compared: a test solution containing 0.3% ClO2, 0.07% cetylpyridinium chloride (CPC), and 0.05% sodium fluoride; a placebo; a solution containing 0.05% CPC; and a control solution of 0.2% chlorhexidine gluconate (CHX). VSC levels were assessed using a Halimeter, and 6 measurements were made from baseline to 3 hours postrinse. The VSC reduction rate of the test mouthrinse was superior to the placebo and the CPC solution. There was no difference between the test solution and the CHX solution in VSC reduction rates immediately postrinse, or at 2 and 3 hours postrinse; both solutions were statistically superior to the placebo and the CPC solution.

Assuntos

Halitose , Antissépticos Bucais , Cetilpiridínio/administração & dosagem , Humanos , Antissépticos Bucais/uso terapêutico , Fluoreto de Sódio/uso terapêutico , Compostos de Enxofre

RESUMO

Natural products have emerged as an effective and low-cost alternative for treating various diseases of the oral cavity. This study aimed to evaluate, through a systematic literature review, if there is scientific evidence ensuring the safe and effective use of natural product(s)-containing mouthwashes as adjunctive treatment of biofilm-induced gingivitis. Searches were conducted in the databases Medline, SciELO, LILACS and Cochrane Library, by using combinations of the key words gingivitis/natural products/phytotherapy/mouthwash, in English, Portuguese and Spanish. Studies published until September 2010 were considered. Four examiners analyzed independently: study design and phase, methodological quality (Jadad scale - JE), experimental product and its concentration, dosing interval and time of usage, as well as employed statistical analysis and clinical outcome of interest. From the 503 articles found, 08 were included in the final review as phase II, controlled, randomized and blind clinical trials, scoring 4 (25%) and 5 (75%) in JE. The main natural products assessed were: Azadirachta indica, Garcinia mangostana, Lippia sidoides, Salvadora persica and Sesamum indicum whose concentration, dosing interval, time of usage and adverse effects varied according to each study. The Plaque and Gingival Index were most employed, as well as α = 5% and paired t, Student's t, Wilcoxon and Mann-Whitney tests. A total of 62.5% and 50% of the products significantly reduced supragingival biofilm and gingivitis, respectively. Mouthwashes containing the essential oil from the leaves of L. sidoides (1%) and the extract from the leaves of A. indica (25%) can be indicated as adjunctive treatment of biofilm-induced gingivitis.

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Os produtos naturais têm surgido como alternativa eficaz e de baixo custo para o tratamento de várias doenças da cavidade oral. Objetivou-se avaliar, a partir de revisão sistemática da literatura, se há evidências científicas garantindo a utilização segura e eficaz de antissépticos bucais contendo produto(s) natural(is) como tratamento adjuvante da gengivite induzida por biofilme. Foram realizadas buscas nas bases de dados Medline, SciELO, LILACS e Cochrane Library, através de combinações usando as palavras-chave gengivite/produtos naturais/ fitoterápicos/bochechos, em Inglês, Português e Espanhol. Consideraram-se os estudos publicados até setembro de 2010. Quatro examinadores analisaram separadamente: desenho e fase do estudo, qualidade metodológica (escala de Jadad - EJ), produto experimental e a concentração, intervalo de administração e tempo de uso, bem como a análise estatística empregada e os resultados clínicos de interesse. Foram encontrados 503 artigos dos quais 08 foram incluídos na revisão final como sendo ensaios clínicos fase II, controlados, randomizados e cegos, marcando 4 (25%) e 5 (75%) na EJ. Os principais produtos naturais avaliados foram Azadirachta indica, Garcinia mangostana, Lippia sidoides, Salvadora persica e Sesamum indicum, cuja concentração, intervalo de administração, tempo de uso, e efeitos adversos, variaram de acordo com cada estudo. Índice de placa e Índice Gengival foram os mais utilizados, bem como α=5% e testes t-pareado, t-Student, Wilcoxon e Mann-Whitney. 62,5% e 50% dos produtos reduziram significativamente a presença de bioflme supragengival e gengivite, respectivamente. Os colutórios contendo o óleo essencial das folhas de L. sidoides (1%) e o extrato das folhas de A. indica (25%) podem ser indicados como tratamento adjuvante da gengivite induzida por biofilme.

Assuntos

Antissépticos Bucais/análise , Produtos Biológicos/farmacologia , Biofilmes/classificação , Gengivite/patologia

RESUMO

Purpose: Antiseptic mouthrinses containing chlorhexidine (CHX) seem to be the most effective chemical agents for plaque control. The CHX concentration is usually 0.12% or 0.20%, but formulations with lower concentrations of CHX (0.05%) in combination with other active agents such as sodium fluoride (0.05%) or cetyl pyridinium chloride (0.05%) (Cpc) are available. The aim of this study was to compare plaque formation at 24 hours after the use of 0.1% CHX mouthrinse-gel and mouthrinses containing 0.12% and 0.05% CHX plus other active agents. Methods: A controlled, randomized, double-blind, crossover clinical trial was designed. Thirty subjects underwent four consecutive experimental phases with four treatments: CHX 0.1% + hydroxymethylcellulose 2.5% (HMC), CHX 0.12% + alcohol, CHX 0.12% + 0.05% sodium fluoride, and CHX 0.05% + 0.05% Cpc. On the day of study, the subjects discontinued all other oral hygiene habits and were randomly assigned for treatment with the experimental mouthwash. Each experimental phase was preceded by a 28-day washout period. Plaque formation was recorded after one undisturbed day. Results: Formulations of CHX 0.12% with alcohol and sodium fluoride and CHX 0.1% + HMC 2.5% reduced de novo plaque formation to a greater extent than the mouthwash with CHX 0.05% + Cpc (P < 0.05). Conclusion: The 0.1% CHX gel presents an anti-plaque efficacy similar to that of mouthwashes containing 0.12% CHX and other active agents, and was more effective at inhibiting plaque formation than the mouthwash containing 0.05% CHX with Cpc.

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Objetivo: Enxaguatórios contendo clorexidina (CHX) parecem ser os mais eficazes agentes químicos para controle de placa. A concentração de CHX geralmente é de 0.12% ou 0.20%, mas formulações de clorexidina em concentrações mais baixas (0.05%) com outros agentes ativos, tais como fluoreto de sódio (0.05%), cloreto de cetilpiridínio (0.05%) estão disponíveis atualmente. Este estudo teve por objetivo comparar a eficácia de gel de clorexidina a 0.1% com enxaguatórios contendo 0.12% e 0.05% CHX e outros agentes ativos, em relação ao crescimento de placa bacteriana em 24 horas. Metodologia: Um ensaio clínico com delineamento cruzado, randomizado, controlado e duplo-cego foi concebido. Trinta sujeitos foram submetidos a quatro fases experimentais consecutivas com quatro tratamentos (bochechos): CHX 0.1% + HMC 2.5%, 0.12% CHX + álcool, CHX 0.12% + 0.05% de fluoreto de sódio e CHX 0.05% + 0.05% cloreto de cetilpiridínio. No dia de estudo, os indivíduos interromperam todas as medidas de higiene oral e foram tratados os bochechos experimentais aleatoriamente. Cada fase experimental foi precedida por um período de 28 dias de washout. A formação de placa foi registrada depois de um dia sem disturbio de formação. Resultados: As formulações de CHX 0.12% com álcool e fluoreto de sódio e gel foram eficientes em retardar a formação de placa e foram superiores (P < 0,05) para a CHX 0.05% com cloreto de cetilpiridínio. Conclusão: O gel de clorexidina 0,1% para bochechos apresenta uma eficácia antiplaca semelhante aos bochechos de 0.12% CHX com outros agentes ativos, sendo superior aos bochechos de clorexidina a 0.05% com cloreto de cetilpiridínio.

Assuntos

Humanos , Masculino , Feminino , Antissépticos Bucais/uso terapêutico , Clorexidina/administração & dosagem , Placa Dentária/prevenção & controle , Método Duplo-Cego

RESUMO

This blind and randomized study tested in vitro, using validated protocols, the anticaries potential of an experimental fluoride mouthrinse. One-hundred enamel slabs, half sound and half with caries-like lesions (carious), all with known surface microhardness (SMH), were submitted to 3 treatment groups: A) a placebo mouthrinse (negative control); B) a positive control mouthrinse containing 0.05 percent NaF; and C) an experimental formulation containing 0.05 percent NaF and cetylpyridinium chloride as an antibacterial substance. To evaluate the formation of F products on enamel, sound (n=10) and carious (n=10) slabs were treated with the formulations during 10 min and loosely and firmly-bound F formed in enamel were determined after extraction with alkali and acid, respectively. To evaluate the inhibition of enamel demineralization, sound enamel slabs (n=10) were treated with the mouthrinse formulations 2x/day during 1 min and subjected to a pH-cycling regimen simulating a cariogenic challenge (demineralization). To evaluate enamel remineralization, the carious slabs (n=10) were submitted to the treatments 3x/day and subjected to a pH-cycling model simulating a remineralizing condition. After 8 days, enamel SMH was determined again and the percentage of SMH loss or SMH recovery was calculated for the sound and carious slabs, respectively. The experimental formulation was superior to the negative control (p<0.05) and equivalent to the positive control (p>0.05) in the formation of F products in enamel, and in the inhibition of enamel demineralization and enhancement of remineralization. These data suggest that the tested experimental fluoride mouthrinse has anticaries potential.

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Este estudo in vitro, cego e randomizado avaliou o potencial anticárie de uma formulação experimental de enxaguatório fluoretado (F), usando protocolos previamente validados. Blocos de esmalte (120), 60 hígidos e 60 com lesão artificial de cárie, com microdureza de superfície (MDS) conhecida, foram submetidos a 3 grupos de tratamentos: A) Enxaguatório placebo (controle negativo); B) Controle positivo contendo NaF 0,05 por cento e C) Uma formulação experimental contendo NaF 0,05 por cento e cloreto de cloreto de cetil-piridínio como antibacteriano. Para testar a capacidade de formação de produtos fluoretados no esmalte, blocos hígidos (n=10) e cariados (n=10) foram tratados durante 10 min e os fluoretos fracamente e fortemente ligados formados foram determinados após extração com álcali e ácido, respectivamente. Para avaliar a inibição da desmineralização do esmalte, os blocos hígidos (n=10) foram tratados com as formulações 2 x/dia durante 1 min e submetidos a um modelo de ciclagem de pH, simulando o processo de desmineralização. Para avaliar a ativação da remineralização do esmalte, os blocos com lesões de cárie (n=10) foram tratados 3 x/dia e submetidos a um modelo de ciclagens de pH, simulando uma condição de remineralização. Após 8 dias, a MDS do esmalte foi novamente determinada e as porcentagens de sua redução ou aumento foram calculadas, respectivamente para os blocos hígidos ou com lesão de cárie. A formulação experimental foi superior ao controle negativo (p<0,05) e equivalente ao positivo (p>0,05), na formação de produtos fluoretados no esmalte e na inibição de sua desmineralização e ativação da remineralização. Conclui-se que o enxaguatório experimental apresenta fluoreto ativo com potencial para controlar cárie dental.

Assuntos

Animais , Bovinos , Cariostáticos/uso terapêutico , Cárie Dentária/prevenção & controle , Esmalte Dentário/efeitos dos fármacos , Fluoretos/uso terapêutico , Antissépticos Bucais/uso terapêutico , Antibacterianos/uso terapêutico , Química Farmacêutica , Fluoreto de Cálcio/análise , Cariostáticos/análise , Cetilpiridínio/uso terapêutico , Cárie Dentária/patologia , Esmalte Dentário/patologia , Fluoretos/análise , Dureza , Concentração de Íons de Hidrogênio , Placebos , Distribuição Aleatória , Método Simples-Cego , Fluoreto de Sódio/uso terapêutico , Fatores de Tempo , Remineralização Dentária

RESUMO

The antimicrobial activity of Curcuma zedoaria (Christm) Roscoe extract against some oral microorganisms was compared with the antimicrobial activity of five commercial mouthrinses in order to evaluate the potential of the plant extract to be incorporated into formulas for improving or creating antiseptic activity. The in vitro antimicrobial efficacy of plant extracts and commercial products were evaluated against Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans using a linear regression method to evaluate the microbial reduction obtained in function of the exposure time, considering as effectiveness a 99.999% reduction in count of standardized microbial populations within 60 seconds. The results showed that the antimicrobial efficacy of Curcuma zedoaria (Christm) Roscoe extract was similar to that of commercial products, and its incorporation into a mouthrinse could be an alternative for improving the antimicrobial efficacy of the oral product.

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A atividade antimicrobiana do extrato de Curcuma zedoaria (Christm) Roscoe contra algumas bactérias da microbiota bucal foi comparada com a atividade antimicrobiana de cinco anti-sépticos comerciais, a fim de avaliar o potencial do extrato vegetal de ser incorporado em formulações com a finalidade de melhorar ou conferir atividade anti-séptica. A eficácia antimicrobiana in vitro do extrato vegetal e produtos comerciais foi avaliada frente a Streptococcus mutans,Enterococcus faecalis,Staphylococcus aureus e Candida albicans, utilizando o método de regressão linear para avaliar a redução microbiana obtida em função do tempo de exposição, considerando como eficácia a redução de 99,999% na contagem de população microbiana padronizada em 60 segundos. Os resultados demonstraram que a eficácia antimicrobiana do extrato de Curcuma zedoaria (Christm) Roscoe foi similar a de produtos comerciais e que sua incorporação em anti-sépticos bucais pode ser uma alternativa para aumentar a eficácia antimicrobiana de produtos para uso oral.