RESUMO
Whole-mounted Malpighian tubules of larvae from two meliponid bee species fixed in acetic acid-ethanol showed apositive cytoplasmic staining with Schiff reagent when submitted to the Feulgen reaction in which acid hydrolysiswas done in 4 M HCl at room temperature. The ability of various treatments applied before the Feulgen acid hydrolysisto abolish this cytoplasmic staining was examined. The aldehyde groups of phospholipids present in the cytoplasm ofthe Malpighian tubules were blocked or removed by sequential treatment with 5% sodium borohydride and acetonechloroform(1:1, v/v) for 15 min each prior to HCl hydrolysis. This treatment is recommended in order to abolish thecytoplasmic (plasmal) reaction and to allow the reliable quantification of DNA by the Feulgen reaction and thediscrimination of nuclear phenotypes in the Malpighian tubules of meliponid bees.
Assuntos
Animais , Aldeídos/isolamento & purificação , DNA , Fosfolipídeos/isolamento & purificação , Lipídeos de Membrana , Ploidias , Túbulos de Malpighi/anatomia & histologia , Túbulos de Malpighi/fisiologia , Abelhas/anatomia & histologia , Abelhas/ultraestrutura , Hidrólise , Microscopia EletrônicaRESUMO
The pollen grains collected by Melipona seminigra merrillae, Melipona compressipes manaosensis, Melipona seminigra seminigra, Frieseomelitta sp. and Scaptotrigona sp. were obtained directly from the corbiculae of the worker bees during one year. Identification of the pollen grains showed that Scaptotrigona sp. was the most generalist bee, followed by Frieseomelitta sp., which together collected more pollen taxa than the three species of Melipona. Pollen from three plant species: Miconia myrianthera, Myrcia amazonica e Tapirira guianensis -were collected by this five bee species. Regarding pollen morphology, no significant correlations were found that could indicate a preference for certain pollen grain forms, external structures or sizes of grains. All five meliponids collected pollen of several forms and sizes. It is indicative that the bees collected pollen in accordance to protoplasmic nutrients. Biochemical analyses of some pollen types carried on the corbiculae of the bees showed a high concentration of total soluble sugars, reductive sugars and starch.
Durante um ano, o pólen transportado por Melipona compressipes manaosensis, Melipona seminigra merrillae, Melipona seminigra seminigra, Frieseomelitta sp. e Scaptotrigona sp. foi coletado diretamente das corbículas das operárias. Feita a identificação dos grãos de pólen constatou-se que a abelha mais generalista foi Scaptotrigona sp., seguida de Frieseomelitta sp., as quais juntas coletaram mais tipos polínicos que as três espécies de meliponas. Duas espécies de plantas: Miconia myrianthera e Myrcia amazonica, tiveram seu pólen coletado pelas cinco espécies de meliponíneos durante o ano todo. Quanto à morfologia do pólen, não houve correlação significativa que comprovasse que as abelhas coletassem o pólen de acordo com a forma, ornamentação e o tamanho dos grãos. As cinco espécies de meliponíneos coletaram pólen de diversas formas e tamanhos. Tudo indica que as abelhas coletam o pólen de acordo com os nutrientes contidos no seu protoplasma. Análises bioquímicas de alguns tipos polínicos encontrados nas corbículas das abelhas apresentaram alto teor de açúcares solúveis totais, açúcares redutores e amido.
RESUMO
The pollen grains collected by Melipona seminigra merrillae, Melipona compressipes manaosensis, Melipona seminigra seminigra, Frieseomelitta sp. and Scaptotrigona sp. were obtained directly from the corbiculae of the worker bees during one year. Identification of the pollen grains showed that Scaptotrigona sp. was the most generalist bee, followed by Frieseomelitta sp., which together collected more pollen taxa than the three species of Melipona. Pollen from three plant species: Miconia myrianthera, Myrcia amazonica e Tapirira guianensis -were collected by this five bee species. Regarding pollen morphology, no significant correlations were found that could indicate a preference for certain pollen grain forms, external structures or sizes of grains. All five meliponids collected pollen of several forms and sizes. It is indicative that the bees collected pollen in accordance to protoplasmic nutrients. Biochemical analyses of some pollen types carried on the corbiculae of the bees showed a high concentration of total soluble sugars, reductive sugars and starch.
Durante um ano, o pólen transportado por Melipona compressipes manaosensis, Melipona seminigra merrillae, Melipona seminigra seminigra, Frieseomelitta sp. e Scaptotrigona sp. foi coletado diretamente das corbículas das operárias. Feita a identificação dos grãos de pólen constatou-se que a abelha mais generalista foi Scaptotrigona sp., seguida de Frieseomelitta sp., as quais juntas coletaram mais tipos polínicos que as três espécies de meliponas. Duas espécies de plantas: Miconia myrianthera e Myrcia amazonica, tiveram seu pólen coletado pelas cinco espécies de meliponíneos durante o ano todo. Quanto à morfologia do pólen, não houve correlação significativa que comprovasse que as abelhas coletassem o pólen de acordo com a forma, ornamentação e o tamanho dos grãos. As cinco espécies de meliponíneos coletaram pólen de diversas formas e tamanhos. Tudo indica que as abelhas coletam o pólen de acordo com os nutrientes contidos no seu protoplasma. Análises bioquímicas de alguns tipos polínicos encontrados nas corbículas das abelhas apresentaram alto teor de açúcares solúveis totais, açúcares redutores e amido.