RESUMO
This study aimed to evaluate the effects of dietary supplementation pelleted Tenebrio molitor (TM) powder with chokeberry by-product (CBP) meal on duck production and meat quality traits. A total of 180 0-day-old Pekin ducks were allocated randomly between three dietary groups viz., control, Treatment 1, and Treatment 2, with three pens per group and twenty birds per pen, characterized by increasing levels of pelleted TM powder with CBP meal in concentration of 0%, 1.5%, and 3%, respectively, and reared until 42 days of age in a completely randomized design. On day 42, the ducks were sacrificed, and breast and thigh muscles were used for meat quality evaluations. Feeding pelleted TM powder with CBP meal resulted in a significant difference in final body weight, weight gain and feed conversion ratio (p<0.05). Additionally, a significant difference was observed in the pH of the breast meat samples (p<0.05). As the only significant difference between the breast and thigh meat, 1,1-diphenyl-2-picryhydrazyl radical scavenging was influenced significantly by the dietary inclusion of pelleted TM powder with CBP meal (p<0.05). However, the values of thiobarbituric acid reactive substances and meat color in both breast and thigh meat were insignificant (p>0.05) different among all groups. To conclude, the inclusion of up to 3 % of pelleted TM powder with CBP meal in the diets of ducks did improve the growth production and antioxidant characteristics effects, which could decrease TBARS and reflected by changes in 1,1-diphenyl-2- picrylhydrazyl free DPPH radical scavenging values.(AU)
Assuntos
Pele , Tenebrio/anatomia & histologia , Carne/análise , PatosRESUMO
Background: Reference values are very important in clinical management of diseased animal. The mutton quality characteristics not only could reflect the breed of sheep, but also allow for a selection of improved meat quality in the breed analyzed with more attractive sensory attributes. The aim of this study was to establish reference values for commonly used hematologic and biochemical parameters and evaluating the amino acid and fatty acid composition of meat quality traits of Tibetan sheep.Materials, Methods & Results: A total of 80 Tibetan sheep were randomly selected. Blood samples were collected used for hematological and biochemical analysis. The contents of mineral elements in serum were also measured. The animals were slaughtered according to commercial procedures. The mutton samples were removed from the Longissimus dorsi (LD) muscles used for amino acid and fatty acid composition analyzed. We have established a locally relevant reference parameters for commonly used hematological and biochemical tests. Most hematological and serum biochemical values were similar to those of yaks and camels, but the Hemoglobin (HGB) concentration was a little higher. The concentrations of Cu, Zn and Se were remarkably lower than critical values in the present study. LD muscle in Tibetan sheep had a higher proportion of total PUFA. We found a large amount of Glu, Lys, Asp, Leu and Arg in the LD muscle of Tibetan sheep. Discussion: Hematological and biochemical parameters are useful tools in measuring the physiological status of animals because they may provide information for diagnosis and prognosis of diseases. Tibetan sheep has survived for millions of generations in this low oxygen condition on the plateau. Tibetan sheep must have evolved exceptional mechanisms to adapt to this extremely inhospitable habitat. The study about Tibetan sheep will undoubtedly facilitate the discovery of potential molecular mechanisms of high-altitude adaptation.[...]
Assuntos
Animais , Aminoácidos/análise , Análise de Alimentos , Carne/análise , Ovinos/sangue , Qualidade dos Alimentos , Ácidos Graxos/análise , Padrões de Referência , TibetRESUMO
Background: Reference values are very important in clinical management of diseased animal. The mutton quality characteristics not only could reflect the breed of sheep, but also allow for a selection of improved meat quality in the breed analyzed with more attractive sensory attributes. The aim of this study was to establish reference values for commonly used hematologic and biochemical parameters and evaluating the amino acid and fatty acid composition of meat quality traits of Tibetan sheep.Materials, Methods & Results: A total of 80 Tibetan sheep were randomly selected. Blood samples were collected used for hematological and biochemical analysis. The contents of mineral elements in serum were also measured. The animals were slaughtered according to commercial procedures. The mutton samples were removed from the Longissimus dorsi (LD) muscles used for amino acid and fatty acid composition analyzed. We have established a locally relevant reference parameters for commonly used hematological and biochemical tests. Most hematological and serum biochemical values were similar to those of yaks and camels, but the Hemoglobin (HGB) concentration was a little higher. The concentrations of Cu, Zn and Se were remarkably lower than critical values in the present study. LD muscle in Tibetan sheep had a higher proportion of total PUFA. We found a large amount of Glu, Lys, Asp, Leu and Arg in the LD muscle of Tibetan sheep. Discussion: Hematological and biochemical parameters are useful tools in measuring the physiological status of animals because they may provide information for diagnosis and prognosis of diseases. Tibetan sheep has survived for millions of generations in this low oxygen condition on the plateau. Tibetan sheep must have evolved exceptional mechanisms to adapt to this extremely inhospitable habitat. The study about Tibetan sheep will undoubtedly facilitate the discovery of potential molecular mechanisms of high-altitude adaptation.[...](AU)
Assuntos
Animais , Ovinos/sangue , Carne/análise , Ácidos Graxos/análise , Aminoácidos/análise , Qualidade dos Alimentos , Análise de Alimentos , Tibet , Padrões de ReferênciaRESUMO
Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study. g.244T>C located at 5'untranslated region (5'UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA. Conclusions Analysis results showed that these three SNPs were significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs). We suggested that CDIPT gene may have potential effects on BMTs and MQTs and can be used for marker-assisted selection.
Assuntos
Animais , Polimorfismo Genético , Bovinos/genética , CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferase/genética , Polimorfismo de Fragmento de Restrição , Expressão Gênica , Indústria da Carne , Análise de Sequência de DNA , Eletroforese em Gel de Ágar , CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferase/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Genótipo , Carne/análiseRESUMO
Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data. Conclusions: The results showed that the G264C SNP and C762T SNP of bovine CMKLR1 were significantly associated with backfat thickness (BFT) and water holding capacity (WHC), respectively.