RESUMO
BACKGROUND Diabetes is a metabolic disorder caused by defects in insulin production and activity. During disease progression, changes in lipid peroxidation cause structural modifications via production of free radicals. Fangchinoline is a well-known alkaloid present in Stephaniae tetrandrine S. Moore, which has demonstrated antioxidant, anticancer, and anti-inflammatory activities. RESULTS The present study analyzed the anti-diabetic and antioxidant effects of fangchinoline in male rats with streptozotocin-induced diabetes. Rats were divided into the following groups: normal control, diabetic, diabetic + fangchinoline 100 mg/kg, diabetic + fangchinoline 200 mg/kg and diabetic + glibencla mide 600 mg/kg. The treatment was administered orally for 45 consecutive days. Lipid peroxidation was substantially increased by >50% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. However, fangchinoline supplementation significantly reduced lipid peroxidation to near normal levels. Reactive oxygen species levels were substantially increased by >500% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. Fangchinoline supplementation reduced reactive oxygen species to near normal levels. Fangchinoline supplementation significantly improved superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels in diabetic rats. Total hexoses, sialic acid, hexosamines, and fucose were increased in diabetic rats, whereas fangchinoline supplementation significantly reduced these total hexoses, sialic acid, hexosamines, and fucose to near normal levels CONCLUSIONS Supplementation with fangchinoline led to significant attenuation of the levels of lipid peroxidation, ROS, and glycoprotein components such as total hexoses, hexosamines, sialic acid, and fucose, while improving antioxidant marker levels
Assuntos
Animais , Masculino , Ratos , Benzilisoquinolinas/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Espécies Reativas de Oxigênio , EstreptozocinaRESUMO
The present work was aimed at studying the antifertility potential of the commonly used herb, rosemary in the male albino rats using electron microscopy as the method of investigation. Ethanolic extract of the rosmary prepared and administered orally in two different doses for a period of three months to the animals. At the end of the experiment animals were sacrificed and testes removed. Sections for the electrone microscopy prepared and changes were observed. The present results showed evident microscopic changes in the testis of the animals received higher dose of the drug. Most of the seminiferous tubules were compressed, having irregular basement membrane and devoid of any spermatogenic cells. The present work revealed a clear morphological evidence of the dose dependent antifertility potential of the rosemary in the male albino rats.
El trabajo tuvo como objetivo estudiar el potencial anti-fertilidad de la hierba de uso común, el romero, en ratas albinas macho utilizando microscopía electrónica como método de investigación. El extracto etanólico del romero se preparó y administró por vía oral a los animales en dos dosis diferentes durante un período de tres meses. Los animales experimentales se sacrificaron y se retiraron sus testículos. Se prepararon secciones para microscopía electrónica y se observaron los cambios. Los resultados mostraron cambios microscópicos evidentes en los testículos de los animales que recibieron una dosis mayor del medicamento. La mayoría de los túbulos seminíferos se observaron comprimidos, con una membrana basal irregular y carente de células espermatogénicas. El presente trabajo revela una clara evidencia morfológica de una posible anti-fertilidad dependiente de la dosis del romero administrada en las ratas albinas macho.