RESUMO
This study explores the biotechnological potential of lignocellulolytic fungi collected in an oak forest. Fungal collections were obtained from natural reserves located in Boyacá-Colombia, ranging from 2700 to 3000 m.a.s.l. Twenty-three strains were isolated on malt agar, molecular characterization was performed, and ligninolytic and cellulolytic enzymatic activities were screened. Several white-rot fungi of biotechnological importance were identified as follows: Trametes sp., Trametes versicolor, Trametes villosa, Pycnoporus sanguineus, Bjerkandera adjusta, Lentinula boryana, Panus conchatus, Antrodia neotropica, Brunneoporus malicola, Laetiporus gilbertsonii, Stereum sp., Ganoderma sp., and Dichomitus sp. The strains T. versicolor 0554 and 0583, T. villosa 0562, and B. adusta 0556 showed the highest response in the qualitative enzymatic assays. These strains were used to determine their ability to decolorate the dyes aniline blue and Congo red, and it was found that T. villosa 0562 reached a level of decolorization close to 90% after 48 h of submerged culture. The fungal strains obtained here could offer alternatives to develop a process to accomplish sustainable development objectives.
Assuntos
Trametes , Madeira , Colômbia , Corantes , Florestas , Madeira/microbiologiaRESUMO
BACKGROUND: An ethnomycological study was conducted to describe the fungus concept and the traditional fungus classification system for the Nahuas of San Isidro Buensuceso, in central Mexico. The study which provides information on the co-existence of various forms of classification, based on both cultural and biological characteristics. METHODS: The research included conducting community interviews and forest forays in the company of mushroom pickers. The triad technique, pile sorting, and fresh mushroom sampling methods were used. Traditional names were analyzed to describe the Nahua classification system for fungi. RESULTS AND CONCLUSION: The triad technique with non-utilitarian stimuli allowed the fungi to be identified as an independent group of plants and animals. The Nahua people of San Isidro classify fungi primarily based on their use, where they grow, and by humoral characteristics. The analysis of the names revealed a classification based on the criteria proposed by Brent Berlin. This study identified the detailed knowledge of fungi in this Nahua community. The criteria used for the recognition of the species are very reliable, since they use organoleptic, ecological, phenological, and morphological characteristics.
Assuntos
Agaricales/classificação , Classificação/métodos , Conhecimento , Etnicidade , Humanos , México , Micologia , Terminologia como AssuntoRESUMO
Resumen Objetivo. Evaluar la potencial actividad degradadora de la cepa comercial Pleurotus ostreatus sobre petróleo crudo como única fuente de carbono. Método. Se empleó un inóculo de 10mg del hongo en medio mínimo líquido de sales con volúmenes de 0,5% y 1% de petróleo en agitación constante durante 21 días a temperatura ambiente, registrando peso seco a los 0, 8,15 y 21 días. Resultados. Se obtuvo un máximo de biomasa 45mg para 0,5% de 39mg para 1%. Esto sugiere que el hongo sufre una adaptación fisiológica para utilizar el petróleo crudo para su crecimiento.
Abstract Objectives. To evaluate the potential crude oil-degrading activity of Pleurotus ostreatus using it as the sole source of carbon and energy for growth. Methods. An inoculum of 10 mg of the fungus in the minimal liquid of salt with 0.5% or 1% crude oil in constant agitation for 21 days was used. Dry weight at 0, 8, 15 and 21 days was obtained. Results. The maximum biomass with a value of 45mg of 0.5% oil treatment registered while the 1% oil treatment reached a value of 39mg. These values suggest physiological adaptation of the fungus to crude oil as its sole carbon source for growth.
Assuntos
Humanos , Técnicas In Vitro , Petróleo , Pleurotus , HidrocarbonetosRESUMO
A lo largo de la historia numerosos investigadores han intentado estudiar la sociología de los hongos, también llamada micosociología que se refiere al estudio y la clasificación de las comunidades fúngicas, sus interrelaciones y su dependencia del medio. La mayoría de estos estudios han sido realizados en Europa, donde destacan los trabajos realizados por Darimont (1973) y García Bona (1977). Son pocos los estudios de carácter micosociológico que describen la interacción de ciertos macromicetos con especies de Nothofagus de América del Sur, destacando los de Godeas et al. (1993 a, b, c) en bosques de Nothofagus de Tierra del Fuego y el de Valenzuela et al. (1998). En esta revisión, se exponen los comienzos históricos de la micosociología, su evolución a lo largo de los años y cómo su enfoque ayuda a comprender el funcionamiento de las comunidades fúngicas. También, se destaca la importancia que tiene para nuestro país realizar estudios micosociológicos, especialmente, en bosques nativos dominados por Nothofagus.
Over time many researchers have tried to study the sociology of fungi, or mycosociology, meaning the study and classification of fungal communities, their inter-relations and their dependence on the medium. The majority of these studies have been carried out in Europe, notably the works of Darimont (1973) and García Bona (1977). There are few mycosociological studies which describe the interaction of certain macromycetes with South American Nothofagus species. Important studies are Godeas et al. (1993 a, b, c), in Nothofagus forests in Tierra del Fuego, and Valenzuela et al. (1998). This review examine the historical beginnings of mycosociology, its evolution over time and how its focus helps to understand the functioning of fungal communities. The autor also stress the importance for Chile of carrying out mycosociological studies, especially in native forests dominated by Nothofagus.