RESUMO

In folk medicine, Vismia guianensis is used to treat skin diseases and mycoses in the Amazon region. We evaluated the anti-Candida activity of the hydroalcoholic extract from the leaves of Vismia guianensis (EHVG). HPLC-PDA and FIA-ESI-IT-MSn were used to chemically characterize EHVG. The anti-Candida activity was determined in vitro by the minimum inhibitory concentrations (MIC) against Candida glabrata (ATCC-2001); Candida albicans (ATCC-90028, ATCC-14053, and ATCC-SC5314), and C. albicans clinical isolates. EHVG effects on adhesion, growth, and biofilm formation were also determined. Molecular docking was used to predict targets for EHVG compounds. The main compounds identified included anthraquinone, vismione D, kaempferol, quercetin, and vitexin. EHVG was fungicidal against all tested strains. C. albicans ATCC 14053 and C. glabrata ATCC 2001 were the most sensitive strains, as the extract inhibited their virulence factors. In silico analysis indicated that vismione D presented the best antifungal activity, since it was the most effective in inhibiting CaCYP51, and may act as anti-inflammatory and antioxidant agent, according to the online PASS prediction. Overall, the data demonstrate that EHVG has an anti-Candida effect by inhibiting virulence factors of the fungi. This activity may be related to its vismione D content, indicating this compound may represent a new perspective for treating diseases caused by Candida sp.

RESUMO

Background: Laparoscopic ovariectomy it is used more than 20 years in veterinary medicine, however, information of this technique in Brazil are rare. Also, the cost for his execution are high, specially the ovarian hemostasis procedure, that depends on highly specialized surgical instruments. The goal of this study is show the results achieved during laparoscopic ovariectomy in mares using a device to apply and cut tie-wraps, developed by the authors and that is in a patent process. Materials, Methods & Results: Four mares with ovarian pathologies were selected for a standing unilateral ovariectomy procedure and access to cavity occurred through three laparoscopic portals. The vessels and ligament were dissected to allow positioning of a polyamide tie-wrap by the device for cutting and applying those seals. Occlusion was made by pushing, and cutting through a rotation on the tie latch. Then, the mesovarium was cut with laparoscopic scissors and ovary removed by a communication between two accesses. Total surgical time and the equipment handling were noted. The average duration of surgical procedures was 80 ± 12.35 min and device handling time since its external preparation, application and cutting the polyamide tie-wraps was 3.4 ± 2.63 min. Only in one of four animals it was necessary to apply more than one tie, since the first promoted incomplete vessels occlusion [...]

Assuntos

RESUMO

Background: Laparoscopic ovariectomy it is used more than 20 years in veterinary medicine, however, information of this technique in Brazil are rare. Also, the cost for his execution are high, specially the ovarian hemostasis procedure, that depends on highly specialized surgical instruments. The goal of this study is show the results achieved during laparoscopic ovariectomy in mares using a device to apply and cut tie-wraps, developed by the authors and that is in a patent process. Materials, Methods & Results: Four mares with ovarian pathologies were selected for a standing unilateral ovariectomy procedure and access to cavity occurred through three laparoscopic portals. The vessels and ligament were dissected to allow positioning of a polyamide tie-wrap by the device for cutting and applying those seals. Occlusion was made by pushing, and cutting through a rotation on the tie latch. Then, the mesovarium was cut with laparoscopic scissors and ovary removed by a communication between two accesses. Total surgical time and the equipment handling were noted. The average duration of surgical procedures was 80 ± 12.35 min and device handling time since its external preparation, application and cutting the polyamide tie-wraps was 3.4 ± 2.63 min. Only in one of four animals it was necessary to apply more than one tie, since the first promoted incomplete vessels occlusion [...](AU)

Assuntos

RESUMO

A limpeza, desinfecção, esterilização e acondicionamento dos materiais cirúrgicos são considerados fatores importantes para o controlo de infecção hospitalar. A abraçadeira autoestática de náilon é um dispositivo projetado para a sua utilização em instalações electrohidráulicas. São fabricados em poliamida e resistentes ao desgaste e aos produtos químicos. Recentemente, este material tem sido utilizado em várias intervenções cirúrgicas na medicina veterinária e na medicina humana. O objetivo do estudo foi avaliar a eficácia dos diferentes métodos de antissepsia e esterilização da abraçadeira autoestática de náilon. Foram definidos quatro grupos das abraçadeiras: Grupo I (grupo controlo positivo) não foi submetido a qualquer método de antissepsia e/ou esterilização; Grupo II foi submetido à esterilização na autoclave; Grupo III submetida à antissepsia no forno de micro-ondas; Grupo IV submetido ao amônio quaternário. Concluiu-se que a esterilização das abraçadeiras autoestáticas de náilon utilizando a autoclave a uma temperatura de 121ºC, durante 15 minutos, e o forno micro-ondas com 100 mL de água posicionada no centro do prato giratório na potência máxima (608,52 watts), durante 10 minutos, foram eficazes na esterilização e antissepsia das abraçadeiras de náilon. Paralelamente, a emersão das abraçadeiras em amônio quaternário na diluição de 4:1, durante 30 minutos, foi eficaz no processo de antissepsia. (AU)

Cleaning, disinfection, sterilization and packing surgical materials are considered important factors for hospital infection control. Nylon ties are devices design for using in electrohydraulic facilities. They are made from polyamide, resistant to wear and chemical agents. Recently, this material has been used in varied surgical interventions, in veterinary medicine and human medicine. The study aimed to evaluate the effectiveness of different methods of antisepsis and sterilization of nylon ties. Was delineate four nylon ties groups. The Group I (control group) wasn´t submitted to any antisepsis and/or sterilization. The group II was submitted to sterilization by autoclave, the Group III and Group IV were submitted to antisepsis by microwave oven and quaternary ammonium, respectively. Was conclude that the nylon ties sterilization with autoclave at 121 oC temperature for fifteen minutes and the microwave oven with 100 mL of water positioned at the center of the revolving plate with a maximum power (608.52 watts) during ten minutes were effective in sterilization and antisepsis of nylon ties. In other hand, the emersion of nylon ties in quaternary ammonium solution with 4:1 dilution, for thirteen minutes, was effective for antisepsis. (AU)

Assuntos

RESUMO

A limpeza, desinfecção, esterilização e acondicionamento dos materiais cirúrgicos são considerados fatores importantes para o controlo de infecção hospitalar. A abraçadeira autoestática de náilon é um dispositivo projetado para a sua utilização em instalações electrohidráulicas. São fabricados em poliamida e resistentes ao desgaste e aos produtos químicos. Recentemente, este material tem sido utilizado em várias intervenções cirúrgicas na medicina veterinária e na medicina humana. O objetivo do estudo foi avaliar a eficácia dos diferentes métodos de antissepsia e esterilização da abraçadeira autoestática de náilon. Foram definidos quatro grupos das abraçadeiras: Grupo I (grupo controlo positivo) não foi submetido a qualquer método de antissepsia e/ou esterilização; Grupo II foi submetido à esterilização na autoclave; Grupo III submetida à antissepsia no forno de micro-ondas; Grupo IV submetido ao amônio quaternário. Concluiu-se que a esterilização das abraçadeiras autoestáticas de náilon utilizando a autoclave a uma temperatura de 121ºC, durante 15 minutos, e o forno micro-ondas com 100 mL de água posicionada no centro do prato giratório na potência máxima (608,52 watts), durante 10 minutos, foram eficazes na esterilização e antissepsia das abraçadeiras de náilon. Paralelamente, a emersão das abraçadeiras em amônio quaternário na diluição de 4:1, durante 30 minutos, foi eficaz no processo de antissepsia.

Cleaning, disinfection, sterilization and packing surgical materials are considered important factors for hospital infection control. Nylon ties are devices design for using in electrohydraulic facilities. They are made from polyamide, resistant to wear and chemical agents. Recently, this material has been used in varied surgical interventions, in veterinary medicine and human medicine. The study aimed to evaluate the effectiveness of different methods of antisepsis and sterilization of nylon ties. Was delineate four nylon ties groups. The Group I (control group) wasn´t submitted to any antisepsis and/or sterilization. The group II was submitted to sterilization by autoclave, the Group III and Group IV were submitted to antisepsis by microwave oven and quaternary ammonium, respectively. Was conclude that the nylon ties sterilization with autoclave at 121 oC temperature for fifteen minutes and the microwave oven with 100 mL of water positioned at the center of the revolving plate with a maximum power (608.52 watts) during ten minutes were effective in sterilization and antisepsis of nylon ties. In other hand, the emersion of nylon ties in quaternary ammonium solution with 4:1 dilution, for thirteen minutes, was effective for antisepsis.

Assuntos

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.

RESUMO

Ovariectomy is a common surgical spaying method in mares, which it leads to short/medium-term masculinization and improves especially their stamina. This study aimed to assess the economic, clinical and histological aspects of the polyamide tie-rap for haemostasis in surgical sterilization of twelve seven-year-old mares, with or without ovary excision. The equines were spayed with polyamide tie-rap for pedicle preventive haemostasis. The left ovary was excised after mesovarium clamping. The polyamide device was also used in the opposite pedicle, though the ovary was not excised. The mean operating cost was determined, the wound healing was assessed and macroscopic and histological evaluation of the pedicles and ovaries were achieved in the post-op. The mean operating cost of the surgical procedure was US$ 69,44. The ovaries were atrophied and with multifocal necrosis areas, firm irregular nodules and follicles. These nodules were histologically recognized as granulomas. The polyamide tie-rap was efficient in controlling hemorrhage and safe for surgical purpose in the experimental model studied. However, it did not interrupt ovarian activity in the non-excised gland.