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The mechanisms of hybridization can be elucidated by analyzing genotypes as well as phenotypes that could act as premating barriers, as the reproductive interactions among heterospecifics can alter the evolutionary history of species. In frogs, hybrids typically occur among species that reproduce explosively (in dense aggregations) with few opportunities for mate selection but are rare in species with elaborate courtship behaviors that may prevent erroneous mating. Using 21 microsatellite markers, we examined hybridization in the prolonged-breeding tree frogs Bokermannohyla ibitiguara and B. sazimai sampled within a contact zone in the Brazilian savanna (72 tadpoles; 74 adults). We also compared acoustic and morphological data. We confirmed both parental species genetically; STRUCTURE results confirmed 14 hybrids, 11 of which were second-generation according to NEWHYBRIDS, all with intermediate values of genetic dissimilarities compared to the parentals. Morphological and acoustic analyses revealed that hybrids showed variable but not necessarily intermediate phenotypes. Moreover, 2 hybrids exhibited call types different from parentals. The reproduction of B. ibitiguara involves territorial and aggressive males, elaborate courtships with acoustic and tactile stimuli, choosy females, and opportunistic strategies. Our study uncovers a rare case of viable hybridization among closely related frogs with such a combination of complex courtship behaviors and mate choice. We discuss the likely directionality and mechanisms behind this phenomenon, and highlight the importance of investigating hybridization even in species that show elaborate reproduction and female choice to advance our understanding of animal diversification.

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Pseudis tocantins is the only frog species of the hylid genus Pseudis that possesses highly heteromorphic sex chromosomes. Z and W chromosomes of Ps. tocantins differ in size, morphology, position of the nucleolar organizer region (NOR) and the amount and distribution of heterochromatin. A chromosomal inversion and heterochromatin amplification on the W chromosome were previously inferred to be involved in the evolution of this sex chromosome pair. Despite these findings, knowledge related to the molecular composition of the large heterochromatic band of this W chromosome is restricted to the PcP190 satellite DNA, and no data are available regarding the gene content of either the W or the Z chromosome of Ps. tocantins. Here, we sequenced microdissected Z and W chromosomes of this species to further resolve their molecular composition. Comparative genomic analysis suggests that Ps. tocantins sex chromosomes are likely homologous to chromosomes 4 and 10 of Xenopus tropicalis. Analyses of the repetitive DNA landscape in the Z and W assemblies allowed for the identification of several transposable elements and putative satellite DNA sequences. Finally, some transposable elements from the W assembly were found to be highly diverse and divergent from elements found elsewhere in the genome, suggesting a rapid amplification of these elements on the W chromosome.

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The Neotropical genus Scinax Wagler currently comprises 127 species of small treefrogs distributed from southern Mexico to Argentina and Uruguay, including some islands such as Trinidad and Tobago, and St. Lucia (Frost 2020). Two major clades are recognized within Scinax, the S. catharinae and the S. ruber clades. The former is composed of two species groups, the S. catharinae and the S. perpusillus groups (Faivovich 2002; Faivovich et al. 2005). The S. perpusillus species group currently comprises 13 species: S. alcatraz (Lutz); S. arduous Peixoto; S. atratus (Peixoto); S. belloni Faivovich, Gasparini Haddad; S. cosenzai Lacerda, Peixoto Feio; S. faivovichi Brasileiro, Oyamaguchi Haddad; S. insperatus Silva Alves-Silva; S. littoreus (Peixoto); S. melloi (Peixoto), S. peixotoi Brasileiro, Haddad, Sawaya Martins; S. perpusillus (Lutz Lutz); S. tupinamba Silva Alves-Silva; and S. v-signatus (Lutz). These species are endemic of the Brazilian Atlantic Forest and are recognized by their intimate association with bromeliads (Fig. 1), in which adults breed and lay their eggs, and tadpoles develop (Peixoto 1987, 1995; Alves-Silva Silva 2009).

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Cosmocercoides meridionalis n. sp. is described from the large and small intestines of 5 species of tree frogs belonging to the families Hylidae and Phyllomedusidae collected from 2 localities in Southern Amazonia. The new species is allocated to the genus Cosmocercoides Wilkie, 1930, due to the presence of papillae in rosettes, which are complex caudal papillae surrounded by punctuations. Cosmocercoides meridionalis n. sp. differs from its congeners by a combination of the following characters: the absence of the gubernaculum, the total length of the female (twice the size of the male), and the presence of 26 rosette papillae with a unique arrangement and distribution pattern: 11 pre-cloacal pairs, 1 ad-cloacal pair, and 1 post-cloacal pair. This is the first species of the genus described from the Amazonia region.

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In this paper we present a phylogenetic analysis of the treefrogs of the Boana pulchella Group with the goals of (1) providing a rigorous test of its monophyly; (2) providing a test of relationships supported in previous studies; and (3) exploring the relationships of the several species not included in previous analyses. The analyses included>300 specimens of 37 of the 38 species currently included in the group, plus 36 outgroups, exemplars of the diversity of Boana and the other genera of the hylid tribe Cophomantini. The dataset included eight mitochondrial genes (12S, 16S, CytB, COI, ND1, tRNAIle, tRNALeu, and tRNAVal) and five nuclear genes (RHO, TYR, RAG-1, CXCR4, SIAH1). The phylogenetic analyses recover the monophyly of the B. pulchella Group with lower support than previous studies, as a result of the inclusion of the B. claresignata Group, which is recovered as its sister taxon. Within the B. pulchella Group, the inclusion of almost all species of the group had little impact on previous notions of its phylogeny, except for the rejection of the hypothesized B. polytaenia Clade (B. goiana and B. phaeopleura are nested in the clade here called the B. prasina Clade), which is redefined. Phylogenetic support is strong for five major clades, which collectively include all but three of the species sampled: the B. balzani Clade (B. aguilari, B. balzani, B. gladiator, B. melanopleura, B. palaestes), the redefined B. polytaenia Clade (B. botumirim, B. buriti, B. cipoensis, B. jaguariaivensis, B. leptolineata, B. polytaenia, B. stenocephala, and two undescribed species), the B. prasina Clade (B. bischoffi, B. caingua, B. cordobae, B. goiana, B. guentheri, B. marginata, B. phaeopleura, B. prasina, B. pulchella, and one undescribed species), the B. riojana Clade (B. callipleura, B. marianitae, B. riojana), and the B. semiguttata Clade (B. caipora, B. curupi, B. joaquini, B. poaju, B. semiguttata, B. stellae, and two undescribed species). The monophyly of the B. prasina + B. riojana Clades, and that of the B. polytaenia + B. semiguttata Clades are well-supported. The relationships among these two clades, the B. balzani Clade, B. ericae + B. freicanecae, and B. cambui (representing the deepest phylogenetic splits within the B. pulchella Group) are recovered with weak support. We discuss the phenotypic evidence supporting the monophyly of the B. pulchella Group, and the taxonomy of several species, identifying three new synonyms of Boana polytaenia, one new synonym of Boana goiana, and one new synonym of B. riojana.

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Boana freicanecae is a medium-sized hylid (males SVL= 37.3-42.2 mm) belonging to the B. pulchella group (Carnaval Peixoto 2004). This species is associated with streams in Atlantic montane forests (450-750 m elevation) located from the Pernambuco Biogeographic Sub-Region (Silva Casteleti 2003), in the states of Pernambuco and Alagoas, northeastern Brazil (Carnaval Peixoto 2004; Cardoso et al. 2006). Currently, B. freicanecae is classified as 'Data Deficient' according to the Brazilian Red List of Threatened species (ICMBio 2018) and many aspects of its natural history (e.g., advertisement call) are poorly known. Here we describe the advertisement call of this treefrog based on calls obtained from two populations.

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Scinax Wagler currently harbors 72 treefrog species distributed throughout the Neotropical region, occurring from Mexico to Argentina and Uruguay, and also in some Caribbean islands (Frost et al. 2020). The genus has a high level of cryptic diversity, with its actual number of species currently considered to be underestimated (Fouquet et al. 2007, Ferrão et al. 2016). Scinax funereus (Cope) is a medium-sized species described from Moyobamba, San Martín Department, northern Peru, with occurrences reported from the Peruvian and Ecuadorian Amazon (Duellman Wiens 1993), southern Colombia (Lynch 2005), and from the Brazilian states of Acre (Bernarde et al. 2011) and Amazonas (Ramalho et al. 2016). Duellman (1978) reported that the advertisement call of S. funereus "consists of a moderately long, rather high-pitched, single note", but as no call recording was obtained at the time, it was not described. Although Read (2000) published the species' call in the audio form in a compact disc (CD), it was not formally described. Given this, herein we provide the first formal description of the advertisement call of S. funereus.

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BACKGROUND: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied. METHODS: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and fluorescence staining) was evaluated on myoblasts of the C2C12 cell line. RESULTS: The MALDI-TOF/MS analysis identified polypeptides and proteins in the aqueous solution of A. siemersi skin secretion. SDS-PAGE revealed the presence of proteins with molecular masses from 15 to 55 kDa. Steroids, but no alkaloids or peptides (less than 5 KDa), were detected using mass spectrometry. Skin secretion revealed the presence of lipids in methanolic extract, as analyzed by CG-MS. This secretion showed hemolytic and phospholipase A2 activities, but was devoid of amidolytic, proteolytic or clotting activities. Moreover, dose-dependent cytotoxicity in cultured C2C12 myoblasts of the skin secretion was demonstrated. Morphological analysis, quantification of lactate dehydrogenase release and fluorescence staining indicated that the cell death triggered by this secretion involved necrosis. CONCLUSIONS: Results presented herein evidence the biochemical composition and biological effects of A. siemersi skin secretion and contribute to the knowledge on the defense mechanisms of casque-headed frogs.

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Background:Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied.Methods:The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and fluorescence staining) was evaluated on myoblasts of the C2C12 cell line.Results:The MALDI-TOF/MS analysis identified polypeptides and proteins in the aqueous solution of A. siemersi skin secretion. SDS-PAGE revealed the presence of proteins with molecular masses from 15 to 55 kDa. Steroids, but no alkaloids or peptides (less than 5 KDa), were detected using mass spectrometry. Skin secretion revealed the presence of lipids in methanolic extract, as analyzed by CG-MS. This secretion showed hemolytic and phospholipase A2 activities, but was devoid of amidolytic, proteolytic or clotting activities. Moreover, dose-dependent cytotoxicity in cultured C2C12 myoblasts of the skin secretion was demonstrated. Morphological analysis, quantification of lactate dehydrogenase release and fluorescence staining indicated that the cell death triggered by this secretion involved necrosis.Conclusions:Results presented herein evidence the biochemical composition and biological effects of A. siemersi skin secretion and contribute to the knowledge on the defense mechanisms of casque-headed frogs.(AU)

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Communication in anurans is mediated mainly by acoustic signals, which are relevant for mating, territoriality, and other social interactions (Wells 2007). Although these signals are usually conspicuous and relatively stereotyped, some Neotropical frogs display noticeable diversity and complexity in their calls. For instance, Bokermannohyla, a genus of gladiator treefrog from the Atlantic Forest, Cerrado, and Caatinga Biomes of Brazil, often have complex vocal repertoires (Carvalho et al. 2012; Nali Prado 2014), whilst some congeners have simple and stereotyped calls emitted continuously (Carvalho et al. 2013; Taucce et al. 2015; Giaretta et al. 2016; Rocha et al. 2016).

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Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied. Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and fluorescence staining) was evaluated on myoblasts of the C2C12 cell line. Results: The MALDI-TOF/MS analysis identified polypeptides and proteins in the aqueous solution of A. siemersi skin secretion. SDS-PAGE revealed the presence of proteins with molecular masses from 15 to 55 kDa. Steroids, but no alkaloids or peptides (less than 5 KDa), were detected using mass spectrometry. Skin secretion revealed the presence of lipids in methanolic extract, as analyzed by CG-MS. This secretion showed hemolytic and phospholipase A2 activities, but was devoid of amidolytic, proteolytic or clotting activities. Moreover, dose-dependent cytotoxicity in cultured C2C12 myoblasts of the skin secretion was demonstrated. Morphological analysis, quantification of lactate dehydrogenase release and fluorescence staining indicated that the cell death triggered by this secretion involved necrosis. Conclusions: Results presented herein evidence the biochemical composition and biological effects of A. siemersi skin secretion and contribute to the knowledge on the defense mechanisms of casque-headed frogs.(AU)

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Background: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied. Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and fluorescence staining) was evaluated on myoblasts of the C2C12 cell line. Results: The MALDI-TOF/MS analysis identified polypeptides and proteins in the aqueous solution of A. siemersi skin secretion. SDS-PAGE revealed the presence of proteins with molecular masses from 15 to 55 kDa. Steroids, but no alkaloids or peptides (less than 5 KDa), were detected using mass spectrometry. Skin secretion revealed the presence of lipids in methanolic extract, as analyzed by CG-MS. This secretion showed hemolytic and phospholipase A2 activities, but was devoid of amidolytic, proteolytic or clotting activities. Moreover, dose-dependent cytotoxicity in cultured C2C12 myoblasts of the skin secretion was demonstrated. Morphological analysis, quantification of lactate dehydrogenase release and fluorescence staining indicated that the cell death triggered by this secretion involved necrosis. Conclusions: Results presented herein evidence the biochemical composition and biological effects of A. siemersi skin secretion and contribute to the knowledge on the defense mechanisms of casque-headed frogs.

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The treefrog Scinax camposseabrai (Bokermann) is endemic to the Brazilian semiarid region and known from the municipalities of Maracás, Igaporã and Curaçá, in the state of Bahia, and from the municipality of Matias Cardoso, northern Minas Gerais state, Brazil (Frost 2019). It was removed from the synonymy of Scinax x-signatus (Spix), recognized as a valid species, and placed in the S. ruber clade (sensu Faivovich et al. 2005) by Caramaschi Cardoso (2006). Taxonomic changes proposed by Duellman et al. (2016) for the genus Scinax were discussed by Lourenço et al. (2016) and Faivovich et al. (2018), and we follow here their suggestion to consider Ololygon and Julianus (erected for the S. uruguayus group) as synonyms of Scinax. This large genus Scinax currently would include 123 species (Frost 2019), many of which have had their larval morphology formerly described. Here, we describe the external morphology of the previously unknown tadpoles of S. camposseabrai.

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Phyllodytes Wagler 1830 is a genus composed of small species (SVL ranging 2.1-4.2 mm) that present odontoids and dwells in South America (Faivovich et al. 2005; Frost 2018). All known species of this genus are bromeligenous using bromeliads as shelter, calling site (males) and as egg deposition site (females). Offspring develops at the water retained in the axils (e.g., Bokermann 1966; Peixoto 1995; Haddad et al. 2013).

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Bokermannohyla ibitipoca is a small-sized hylid frog (males reaching up to 43 mm in snout-vent length) of the B. circumdata group (Faivovich et al. 2005). This species was described from the Ibitipoca State Park (Municipality of Lima Duarte) in southern Minas Gerais, at the elevation of 1200 m. Bokermannohyla ibitipoca is restricted to the Mantiqueira Complex area (Caramaschi Feio, 1990), where it occurs in primary and secondary gallery forests. This species is found on the ground or on the leaf-litter near water and uses permanent streams for calling and reproduction. Here, we quantitatively describe the release call of B. ibitipoca, with comments on similarity to its advertisement call. Although used less frequently than advertisement calls, release calls have been used to diagnose species and infer phylogenetic relationships (Sullivan Malmos, 1994; Stöck et al. 2000; di Tada et al. 2001; Wogel et al. 2004; Márquez Eekhout 2006; Oliveira et al. 2012; Sanabria Quiroga 2012; Grenat Martino 2013).

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Abstract Vocalizations are an important trait for the identification of cryptic and/or closely related amphibian species. Different vocalizations also contribute to partitioning of the acoustic space by sympatric species. This study aimed to describe the advertisement calls of anurans in a pond of the municipality of Floriano, State of Piauí, Brazil, and infer the acoustic niche partitioning of amphibians. Euclidean distance was used in a cluster analysis approach to infer the acoustic similarities among species. Thirteen species were analysed: Boana raniceps, Dendropsophus nanus, D. rubicundulus , D. minutus, Leptodactylus fuscus, L. troglodytes, L. vastus, Pithecopus nordestinus , Physalaemus cuvieri, P. nattereri, Pleurodema diplolister, Proceratophrys cristiceps and Scinax ruber. From these, six showed more than 90% of acoustic overlap: P. nattereri , P. cuvieri, L. fuscus and L. vastus (Leptodactylidae); and, D. nanus and D. rubicundulus (Hylidae). Despite the acoustic similarities among these six species, the acoustic interference was reduced due to the small number of sympatric species and to distinct features on carrier frequency such as dominant frequency and the degree of modulation in the frequency. Environmental factors limit the periods and sites of reproduction respectively, which may maintain the low anuran diversity and consequently reduce acoustic overlap.

Resumo A vocalização é um componente importante na identificação de espécies crípticas e/ou proximamente relacionadas de anfíbios anuros. Diferenças na bioacústica também contribuem para o particionamento do espaço acústico entre espécies simpátricas. Este estudo descreve o canto de anúncio de anuros em uma lagoa temporária do município de Floriano, Estado do Piauí, Brasil, além de buscar inferir o particionamento do nicho acústico entre essas espécies. Para tanto, foi utilizada a distância Euclidiana para compor uma análise de conglomerados e avaliar a similaridade/dissimilaridade da bioacústica da anurofauna. Treze espécies foram amostradas: Boana raniceps, Dendropsophus nanus, D. rubicundulus , D. minutus, Leptodactylus fuscus, L. troglodytes, L. vastus, Pithecopus nordestinus , Physalaemus cuvieri, P. nattereri, Pleurodema diplolister, Proceratophrys cristiceps e Scinax ruber. Destas, seis apresentaram mais de 90% de sobreposição acústica: P. nattereri, P. cuvieri, L. fuscus e L. vastus (Leptodactylidae); e, D. nanus e D. rubicundulus (Hylidae). Apesar da similaridade acústica entre essas seis espécies, a interferência é reduzida devido ao pequeno número de espécies simpátricas e certas características distintas na frequência do canto, tais como: frequência dominante e sua modulação. Fatores ambientais específicos do semiárido limitam os períodos e sítios reprodutivos, o que mantém baixa diversidade de espécies e consequentemente reduz a sobreposição no nicho acústico.

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The Neotropical genus Aparasphenodon Miranda-Ribeiro is composed of five recognized species commonly referred as "casque-headed frogs" (Frost 2018). Aparasphenodon brunoi Miranda-Ribeiro occurs in Atlantic Forest lowlands from the states of São Paulo to Bahia, Brazil (Feio et al. 1998; Mollo Neto Teixeira Jr. 2012; Ruas et al. 2013). The biology of most Aparasphenodon species is poorly known. Within the genus, until now only Aparasphenodon arapapa Pimenta, Napoli Haddad has its call formally described (Lourenço-de-Moraes et al., 2013, Forti et al., 2018, Guerra et al., 2018). Herein, we describe the advertisement call of Aparasphenodon brunoi Miranda-Ribeiro.

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Based on morphological data collected from treefrogs related to Sarcohyla hazelae, we describe a new species of the genus Sarcohyla from the cloud forest of the Sierra Madre del Sur of Guerrero, Mexico. We compare physical charactersitics of this new species to its closest relatives within the genus Sarcohyla, including dorsal and ventral coloration, head shape, tympanum distinctiveness, morphometrics and the condition of the tubercles on hands and feet. We analyze accoustic data from the advertisement call of males of the new species. We discuss the relationship of the species described herein with several of its cogeners, plus we resurrect the Sarcohyla hazelae group for these frogs. We describe habitat and distribution species related to Sarcohyla hazelae and also comment on the conservation priorities of these frogs.

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Analysing how seasonality shapes abundance patterns fosters understanding of the processes related to amphibian community assemblies. In this study we analyse the relationship between local seasonal patterns of abundance within the anuran community of the Monte Zerpa Cloud Forest, Mérida Mountain Range (Cordillera de Mérida), in the Venezuelan Andes. We hypothesized that variation in precipitation, temperature, and relative humidity affects the temporal abundance patterns of anurans. Data collection was performed through nocturnal biweekly inspections from 2002 to 2003. Air temperature, relative humidity, and monthly precipitation were considered as variables of climatic seasonality. Generalized linear models were used to evaluate the effect of climatic variables on anuran abundance across seasons. Overall, 542 individuals and four anuran species were recorded in stream tributaries only. The local anuran community was comprised of Hyalinobatrachium duranti, Hyloscirtus platydactylus, Hyloscirtus jahni, and Pristimantis vanadisae. The most abundant species were H. duranti (288 individuals) and H. platydactylus (145 individuals), representing 53% and 27% of the total anuran abundance, respectively. Differences in abundance between species were observed. Although the total abundance of anurans was higher during the low precipitation season, no significant differences between the two seasons were detected. The variation in anuran abundance was explained by relative humidity and temperature. Our results suggest that the highest abundance of anurans can be expected when temperatures reach favourable levels (15- 17°C), relative humidity increases, and precipitation remains constant.

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El aumento de los sonidos de origen antropogénico, como los ruidos del tráfico, contribuyen a la contaminación acústica, que produce un efecto nocivo en el canto de los vertebrados. Comparamos la vocalización de machos de Scinax nasicus (Cope, 1862) en ambientes naturales (como referencia o control, Sitio A) y sitios afectados por ruidos de tráfico (Sitio B). La estructura de la vocalización se registró y amplificó en sonogramas (software Raven Pro 1.5). Se midieron siete variables de su vocalización: duración (s), número de notas, número de pulsos por nota, frecuencia máxima, mínima y dominante (kHz) y amplitud (dB). Además, en cada sitio se midió el ruido de fondo (frecuencia fundamental, la F0 y amplitud, dB). La amplitud del ruido de fondo alcanzó valores más altos (68.02 dB) en el Sitio B, en el Sitio A fue menor (34.81 dB). Por lo tanto, el F0 en el Sitio A fue de 6.28 kHz y en el Sitio B fue de 4.15 kHz. Las vocalizaciones de esta rana en el ambiente con ruido de tráfico (Sitio B) se caracterizaron por menor duración (s) y número de pulsos por nota, mayor amplitud (dB) y frecuencias máximas y dominantes más altas (kHz), baja frecuencia mínima en comparación con el ambiente control (Sitio A). Nuestro estudio resaltó que los machos de S. nasicus cambian su estructura vocal en estanques de ruido de tráfico, principalmente por ajuste vocal de sus frecuencias y amplitud para contrarrestar el efecto de enmascaramiento del ruido. Finalmente, el monitoreo acústico de anuros en ambientes ruidosos debe considerar el solapamiento espacial, temporal y espectral entre ruido acústico específico y el comportamiento de la especie.(AU)

Increased anthropogenic-made sounds such as traffic noises contribute to acoustic pollution, which produces deleterious effect on song-vertebrates. We compared the advertisement call of Scinax nasicus (Cope, 1862) males in natural (as a reference or control, Site A) and Sites affected by traffic noises (Site B). Call structure was recorded and it was amplified in sonograms (software Raven Pro 1.5). Seven variables were measured on its advertisement call: duration (s), number of notes, number of pulses per note, maximum and minimum frequency (kHz), dominant frequency (kHz) and amplitude (dB). In addition, at each Site the background noise (the fundamental frequency, F0 and amplitude, dB) was measured. The amplitude of background noise reached higher values (68.02 dB) in Site B, while in Site A was lower (34.81 dB). Thus, the F0 in Site A was 6.28 kHz and in Site B it was 4.15 kHz. Frog call in noisy environment (Site B) were characterized by lesser duration (s) and number of pulses per note, higher maximum and dominant frequencies (kHz), lower minimum frequencies, and amplitude (dB) when compared with control environment (Site A). Our study highlights, that S. nasicus males shift their vocal structure in traffic noisy ponds, mainly by vocal adjust of their frequencies and amplitude to counteract masking effect. Finally, acoustic monitoring of anurans on noise environments should be considering the spatial, temporal and spectral overlap between noise and species-specific acoustic behaviour.(AU)

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