RESUMO
I studied a Brazilian population of Holophrya teres (Ehrenberg, 1834) Foissner, Berger and Kohmann, 1994, using live observation, morphometry, silver impregnation, and scanning electron microscopy. This showed a fair similarity with European populations, especially in having a large (about 13â¯×â¯5⯵m in vivo) micronucleus pyriform in broad-side view and cuneate in narrow-side view. Several new structures were discovered, viz., an internal oral basket, teeth on anterior end of the oral basket rods, and a buccal seal closing the oral basket when not feeding. Based on this knowledge, I provide an improved diagnosis for H. teres, which can be used as template in further species descriptions. Depending on brosse implantation in the somatic ciliature, the genus was split in Holophrya, Hillerophrya nov. gen., Vdacnyophrya nov. gen., and Bardeleophrya nov. gen. Twenty-one Prorodon species were combined with the holophryid genera, using brosse and thick oral basket rods as main markers. This revealed two new species, viz., Holophrya longiarmata nov. spec. and H. agamalievi nov. spec.