RESUMO
Primitive neuroectodermal tumors (PNETs) belong to the Ewing sarcoma family of tumors. These lesions are highly aggressive and are usually found in paravertebral regions, lower limbs, and thorax. However, abdominal PNETs are extremely rare, and only 3 cases of pediatric PNET of the liver have been previously reported. Most patients exhibit symptoms associated with mass effect, due to rapid tumor growth and dissemination. Therefore, an appropriate differential diagnosis is of pivotal importance in order to initiate the corresponding treatment. Here we report the case of a 4-year-old female patient who was diagnosed with PNET of the liver, and we discuss the analysis of focal liver lesions and differential diagnosis in pediatric patients.
RESUMO
The Brazilian coast of the Rio de Janeiro State has bays of great economic, ecological and social importance. However, these ecosystems have been suffering intense anthropogenic influence, mainly due to the contamination by polycyclic aromatic hydrocarbons (PAHs) from urban-industrial activities. Moreover, PAHs are organic pollutants of high toxicity and carcinogenicity causing global concern to human and environmental health. This study evaluated on catfish (Genidens genidens) a set of key parameters (sex, morphometric traits, condition factor (K), PAH metabolites in gallbladder, frequency of micronucleus (MN) and erythrocytic nuclear abnormalities (ENA) in blood. In addition we also evaluated histopathological hepatic effects, Ethoxyresorufin-O-deethylase (EROD) activity and Benzo(a)pyrene diol epoxide (BPDE)-DNA adducts) in liver samples, in order to indicate the fish health status and environmental pollution levels of three main Bays (Guanabara, Sepetiba and Ilha Grande) of the Rio de Janeiro State, in the Southeast of Brazil. In general, the worst physical and metabolic conditions in catfishes were evidenced in Guanabara Bay, possibly indicating the highest level of contamination by PAHs. Contrary evidence was observed in Ilha Grande Bay, showing lower biological changes in G. genidens. However in Sepetiba Bay, the influence of PAHs contamination showed the highest hepatic lesions in catfishes, prevailing foci of cellular alterations, megalocytic hepatosis and hydropic vacuolations. The employability of a set of biomarkers on catfish was efficient for screening pollution for PAHs in tropical environments. This reinforces the need for effective actions of monitoring and conservation strategies of bays of the Rio de Janeiro State (Brazil), in order to ensure quality and health to both human and environment.
Assuntos
Peixes-Gato , Monitoramento Ambiental , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Baías , Brasil , EcossistemaRESUMO
Due to its chemical composition and use in folk medicine, the dry standardized extract of Apeiba tibourbou Aubl. (Tiliaceae) leaves (DSEAT) was tested to assess its hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The animals were treated with DSEAT previously for 7 days, at doses of 25, 50, 100, 200, and 400 mg/kg and 18 mg/kg of rosmarinic acid; the liver damage was induced by administering CCl4 intraperitoneally (i.p.) at days 3 and 7, and 1 h before treating with DSEAT. The hepatoprotective activity was assessed using various biochemical assays such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyltransferase (GGT), malondialdehyde (MDA) and histopatological studies. DSEAT doses of 400, 200, and 100 mg/kg were not capable of protecting the liver against CCl4. However, the dose of 50 mg/kg reduced AST by 31.50% the dose of 25 mg/kg reduced GGT by 57.18% compared to the CCl4 (p < 0.05). In the liver, DSEAT dose of 50 mg/kg and rosmarinic acid reduced MDA by 27.45% and 63.61%, respectively, whereas in plasma, MDA was reduced in all the groups treated with DSEAT and rosmarinic acid. In conclusion, DSEAT exhibits hepatoprotective effect only at low doses and antioxidant activity in vivo after peroral administration. The experimental protocol was approved by the Animal Research Ethics Committee of UFG (CEUA, no. 177/2011).
Assuntos
Animais , Camundongos , Intoxicação por Tetracloreto de Carbono/terapia , Intoxicação por Tetracloreto de Carbono/veterinária , Malondialdeído , Peroxidação de Lipídeos , Tiliaceae/química , Antioxidantes , Doença Hepática Induzida por Substâncias e Drogas/veterináriaRESUMO
Due to its chemical composition and use in folk medicine, the dry standardized extract of Apeiba tibourbou Aubl. (Tiliaceae) leaves (DSEAT) was tested to assess its hepatoprotective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The animals were treated with DSEAT previously for 7 days, at doses of 25, 50, 100, 200, and 400 mg/kg and 18 mg/kg of rosmarinic acid; the liver damage was induced by administering CCl4 intraperitoneally (i.p.) at days 3 and 7, and 1 h before treating with DSEAT. The hepatoprotective activity was assessed using various biochemical assays such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyltransferase (GGT), malondialdehyde (MDA) and histopatological studies. DSEAT doses of 400, 200, and 100 mg/kg were not capable of protecting the liver against CCl4. However, the dose of 50 mg/kg reduced AST by 31.50% the dose of 25 mg/kg reduced GGT by 57.18% compared to the CCl4 (p < 0.05). In the liver, DSEAT dose of 50 mg/kg and rosmarinic acid reduced MDA by 27.45% and 63.61%, respectively, whereas in plasma, MDA was reduced in all the groups treated with DSEAT and rosmarinic acid. In conclusion, DSEAT exhibits hepatoprotective effect only at low doses and antioxidant activity in vivo after peroral administration. The experimental protocol was approved by the Animal Research Ethics Committee of UFG (CEUA, no. 177/2011).(AU)