RESUMO
BACKGROUND: Oral cancer is one of the common malignant tumors of the head and neck. However, current treatments have numerous side effects, and drugs from natural sources may have better therapeutic potential. This research investigated the induction of apoptosis by α-hederin (α-HN), a constituent of Pulsatilla chinensis (Bunge) Regel, in the oral cancer cell line SCC-25 and its underlying mechanism. RESULTS: SCC-25 cells were treated with 50, 100, and 200 µmol/L α-HN. Cell proliferation; extent of apoptosis; activities of caspases-3, 8, and 9; and the expression of Bcl-2, Bax, phosphorylated (p)-phosphoinositide 3-kinase (PI3K), p-Akt, and p-mammalian target of rapamycin (mTOR) proteins were determined using the 3-(4,5)-2-thiazole-(2,5)-diphenyl tetrazolium bromide, flow cytometry, caspase activity detection kits, and western blot assays, respectively. The results showed that the proliferation of SCC-25 cells in the α-HN-treated groups decreased significantly, and the inhibitory effect was time and concentration dependent. Compared with cells in the control group, the extent of apoptosis increased significantly, caspase-3 and -9 activities were significantly enhanced, and the Bcl-2 level was lowered and the Bax level was elevated significantly in SCC-25 cells treated with α-HN for 48 h (P b 0.05). The expression of p-PI3K, p-Akt, and p-mTOR was also significantly lower in SCC-25 cells treated with α-HN than that in the control group (P b 0.05). CONCLUSION: These results indicate that α-HN can inhibit proliferation and induce apoptosis of SCC-25 cells and may exert these effects by inhibiting the PI3K/Akt/mTOR signaling pathway.
Assuntos
Ácido Oleanólico/análogos & derivados , Saponinas/farmacologia , Neoplasias Bucais/metabolismo , Apoptose/efeitos dos fármacos , Ácido Oleanólico/metabolismo , Ácido Oleanólico/farmacologia , Saponinas/metabolismo , Transdução de Sinais/efeitos dos fármacos , Sobrevivência Celular , Western Blotting , Fosfatidilinositol 3-Quinases/metabolismo , Caspases , Pulsatilla , Proliferação de Células/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Citometria de Fluxo , Neoplasias de Cabeça e Pescoço/metabolismoRESUMO
Se realizó un estudio descriptivo, transversal y retrospectivo de 23 pacientes con tumores de cabeza y cuello, atendidos en el Servicio de Oncología Pediátrica del Hospital Infantil Sur Docente Dr Antonio María Béguez César de Santiago de Cuba durante el período 2000-2016. En la casuística predominaron el grupo etario de 10-14 años (39,1 por ciento), el sexo masculino, la localización nasofaríngea (39,1 por ciento), el linfoma no Hodking y el carcinoma papilar como variedades histológicas, el estadio clínico III y el mayor número de pacientes diagnosticados en 2013 y 2014
A descriptive, cross-sectional and retrospective study of 23 patients with head and neck tumors, assisted in the Oncology Pediatric Service of Dr Antonio María Béguez César Southern Teaching Children Hospital was carried out in Santiago de Cuba during 2000-2016. In the case material there was a prevalence of the 10-14 age group (39.1 percent), male sex, nasopharingeal localization (39.1 percen), non Hodking lymphoma and papillary carcinoma as histological varieties, phase III clinical stage and the highest number of patients diagnosed in 2013 and 2014