RESUMO

The study of animal behavior has been carried out for many years in order to understand physiological and pathological factors, so that work with animals could be developed to evaluate animal well-being. The Arouca Law emerged in Brazil, which regulates the use of animals in teaching and research. To further assist in the development and analysis of changes that can cause pain in animals, researchers developed the Grimace Scale, which shows, through assessment, facial changes in animals, allowing researchers to have ways of controlling the animals' distress. Therefore, this work aimed to show the clinical scores and changes of mice when infected with rabies, SARS-COV-2 and cerebral malaria. We showed that animals infected with the pathogens rabies, SARS-COV-2 and cerebral malaria, mainly present weight loss, muscular and postural changes. Finally, understand the importance of recognizing changes in behavior, enabling the relief of pain and suffering in animals used in research. The premise of animal welfare must always prevail over the study.

O estudo do comportamento animal acontece a muitos anos afim de entender os fatores fisiológicos e patológicos, para que os trabalhos com animais sejam desenvolvidos avaliando o bem estar animal, para tanto surge no Brasil a Lei Arouca que regulamenta a utilização de animais em ensino e pesquisa. Para auxiliar ainda no desenvolvimento e análise das alterações que podem causar dor nos animais os pesquisadores desenvolveram a Escala de Grimace, que mostra por meio de avaliação das alterações faciais dos animais, permitindo que os pesquisadores tenham formas de controlar o distresse dos animais. Diante disso, este trabalho teve como objetivo mostrar os escore clínicos e alterações dos camundongos quando infectados com raiva, SARS-COV-2 e Malária cerebral. Evidenciamos que os animais infectados com os patógenos da raiva, SARS-COV-2 e Malária cerebral, apresentam principalmente perda de peso, alteração muscular e postural. Por fim, compreender a importância de reconhecer as alterações de comportamento, possibilitando o alivio da dor e sofrimento dos animais usados em pesquisa. A premissa do bem-estar animal deve sempre prevalecer sobre o estudo.

RESUMO

The present study investigated the influence of oral health on horses' facial expression, reactivity of the acupuncture points and the perception of trainers and owners. We investigated 87 adult horses involved in sports or working activities. Pain was scored in person and by photography by an evaluator "blinded" to the animals' dental condition, using the Horse Grimace Scale (HGS). The acupuncture examination was performed by the same evaluator. Animals were evaluated before (T0) and 15 days (T1) after dental treatment. All horses included in the study had dental disorders. Statistically significant results were seen in median HGS scoring (3 vs. 1, p = 0.001) and number of reactive acupoints (11.2±5.6 vs. 4±2.9, p = 0.001). Additionally, complaints by the trainers/owners were lower at T1 compared with T0. There was a reduction in the pain reaction to application of pressure on following acupoints: dental point (44 vs. 4), Stomach-7 (31 vs. 3), Tiple-Heater (TH)-17 (27 vs. 4), and TH-16 (22 vs. 4). We concluded that acupuncture can be an adjunct method for diagnosis of dental disorders and treatment follow-up.

Este estudo avaliou a influência da saúde bucal na saúde geral de cavalos, por meio da expressão facial, do exame de acupuntura e da percepção geral dos treinadores/proprietários. Avaliaram-se 87 cavalos adultos, envolvidos em atividades esportivas ou de trabalho. A dor foi avaliada pela Horse Grimace Scale, presencialmente e por fotografia, por um avaliador que desconhecia a condição dos animais. Os exames de acupuntura foram realizados sempre pelo mesmo avaliador. As avaliações ocorreram antes (T0) e 15 dias (T1) após o tratamento odontológico. Todos os cavalos estudados apresentavam alterações odontológicas. Observou-se redução significativa na mediana do escore de dor (3 vs. 1, P = 0,001), no número de acupontos reativos (11,2±5,6 vs. 4±2,9, P = 0,001) e nas reclamações dos responsáveis em T1 em relação a T0. Também houve redução na sensibilidade nos acupontos 'ponto do dente' (44 vs. 4), estômago-7 (31 vs. 3), triplo aquecedor (TA)-17 (27 vs. 4) e TA-16 (22 vs. 4). Concluindo, o presente estudo mostrou que a acupuntura pode auxiliar no diagnóstico de problemas odontológicos e no acompanhamento pós-tratamento da cavidade bucal.

Assuntos

RESUMO

One of the most controversial aspects of the use of animals in science is the production of pain. Pain is a central ethical concern. The activation of neural pathways involved in the pain response has physiological, endocrine, and behavioral consequences, that can affect both the health and welfare of the animals, as well as the validity of research. The strategy to prevent these consequences requires understanding of the nociception process, pain itself, and how assessment can be performed using validated, non-invasive methods. The study of facial expressions related to pain has undergone considerable study with the finding that certain movements of the facial muscles (called facial action units) are associated with the presence and intensity of pain. This review, focused on rodents, discusses the neurobiology of facial expressions, clinical applications, and current research designed to better understand pain and the nociceptive pathway as a strategy for implementing refinement in biomedical research.

RESUMO

The study evaluated the effect of a 1/10 dose of flunixin meglumine administered into the governing vessel 1 (GV1) acupoint in horses that underwent castration. Twenty animals received 0.02 mg/kg detomidine intravenously, followed by 2.2 mg/kg ketamine and 0.1 mg/kg diazepam by the same route, and also a local anesthesia with 30 mL lidocaine. As postoperative analgesia, the animals received 1.1 mg/kg flunixin meglumine IV (FIV) or 0.11 mg/kg flunixin meglumine into the GV1 acupoint (FGV). Behavioral parameters were assessed 12 hours before the procedure (baseline) and at 4, 6, 12, and 24 hours after surgery; physiological parameters were measured at baseline and at 2, 4, 6, 8, 10, 12, 16, and 24 hours after surgery. The groups did not differ regarding pain scores. Heart rate was higher in the FIV group than in the FGV group 2 hours after surgery (46 ± 5.2 bpm vs. 37 ± 8.2 bpm); gut sounds decreased at 2, 4, and 6 hours in both groups. The temperature showed a decrease after 2 hours compared with baseline in the FGV group, and the systolic blood pressure was higher in the FGV group than in the FIV group at 8 hours (158 ± 18.1 mmHg vs. 134 ± 14.5 mmHg), 10 hours (157 ± 15.5 mm Hg vs. 130 ± 11.5 mmHg), and 12 hours (151 ± 18.7 mmHg vs. 134 ± 15.8 mmHg). Pharmacopuncture was as effective as conventional dose and route of flunixin meglumine in horses that underwent elective castration under those conditions.

Assuntos

RESUMO

Routine laboratory animal tests necessary to assess the toxicity of snake venoms and the preclinical neutralizing ability of antivenoms and other inhibitory substances induce significant pain and distress. This has prompted initiatives to introduce the routine use of analgesia. In this study, the analgesic effect of morphine and tramadol was assessed in tests assessing the lethal, hemorrhagic, myotoxic and edema-forming activities of the venom of the viperid snake Bothrops asper. The Mouse Grimace Scale (MGS) and mouse-exploration activity were used to assess pain and its inhibition by the analgesics. Results demonstrate that tests assessing lethality and myotoxicity induce higher levels of pain than assays quantifying hemorrhagic and edema-forming activities. Our observations also indicate that pretreatment of mice with both analgesics, at the doses used, were similarly effective in reducing the MGS magnitude and increase mouse-exploration activity after the administration of B. asper venom. Moreover, the analgesic effect of both drugs was more evident in the myotoxic and lethality assays. Combined with previous observations showing that these analgesics do not alter the extent of toxic effects induced by B. asper venom, our results strongly indicate that the use of analgesia (using either morphine or tramadol) should be considered in the routine assessment of venom toxicity and antivenom efficacy.

Assuntos