RESUMO

The current study evaluated the effects of supplementing cassava root silage (CRS) to dairy cows grazing on Megathyrsus maximus cv Mombasa on nutrient intake and digestibility, as well as on milk production and composition. Ten primiparous Girolando cows with average body weight ± (SEM) of 373.45 ± (63.55) kg were used in a replicated 5 × 5 Latin square. Animals were subjected to five treatments: (I) grazing cows without supplementation (WCS); (II) grazing cows provided with 5 kg DM of supplement without CRS (0 g/kg DM of CRS) or including (III) 260, (IV) 520, and (V) 780 g/kg DM of CRS. Statistical analyses were performed using the PROC MIXED of SAS with significance at P < 0.05. Intake of neutral detergent fiber (NDF) and ether extract decreased (P < 0.01), while intake of non-fiber carbohydrates increased (P < 0.01), with increased CRS in the diets. Total DM intake and digestibility of DM, and digestibility of nutritional components were lower (P < 0.03) in WCS animals compared to supplemented animals, except for intake and digestibility of NDF, which was the opposite. Milk yield (MY) and fat corrected milk (FCM), as well as all milk components were unaffected (P > 0.05) by CRS inclusion. In contrast, MY, FCM, protein, lactose, casein, and non-fat milk solids (NFMS) were greater for animals that received supplementation (P < 0.05), compared to animals WCS. Milk fat and total dry extract (TMS) did not differ (P > 0.11) between two groups. In conclusion, CRS may be a potential corn meal replacer in the supplement of dairy cows under tropical conditions.

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This study aimed to evaluate the behavior and thermal comfort of 20 Girolando cows (5/8-H/G), with light and dark coats, in the wettest period of the year, in Santarém, Pará, Brazil, in pasture with access to shade, and plenty of drinking water and mineral salt. Animal behavior categories were computed for 12 h a day, on 3 days in a row, by trained observers. Three day shifts were considered: Morning (6:00 a.m. to 9:55 a.m.), Intermediate (10:00 a.m. to 01:55 p.m.) and Afternoon (2:00 p.m. to 05:55 p.m.). The Temperature Index (TI), the Black Globe Humidity Index (BGHI) and the Comfort Index (CI) were calculated to measure thermal comfort. At all times studied, BGHI pointed that the environment was outside the thermal comfort zone. Dark-coated animals spent more 34.26% of the time in activities in the shade. The light-coated animals remained more 11.88% of the time in the sun, performing their natural behaviors. Both light and dark coat animals remained more 77 and 74.44% of the time in the sun, respectively. The behavior "in the sun while grazing" was the most evident, in both coats, in the studied shifts. The behaviors "in the shade while walking" and "in the shade while standing idle" were more evident (p < 0.01) in dark-coated cattle. The grazing behavior was higher in animals with dark coat (p < 0.05). In all evaluated shifts, there was a positive correlation between the behavior "in the sun while grazing" with the CI (r = 0.44211; p < 0.0305). Behaviors performed in the shade, such as "idleness while lying down," "ruminating while lying down and standing up," and behaviors "in the sun," "idleness while lying down" and "ruminating while lying down," were negatively correlated with CI. It is concluded that, even in the wettest period of the year, in the Eastern Amazon, Girolando dairy cows are exposed to hot environments, which causes thermal discomfort and changes in their natural behavior, as they spend more time standing in shaded areas, usually in rumination. Also, light-coated cows spend more time in the sun, while dark-coated cows spend more time in the shade. Thus, light-coated cows tend to have health and zootechnical performance negatively affected.

RESUMO

This study aimed to investigate the difference between Holstein and Holstein × Gyr breeds on feeding behavior and performance of heifers grazing temperate pasture. The experiment was carried out in 89 days, split into 14 days of adaptation, and 3 periods of 25 days. Two treatments were used: Holstein (HOL; n = 7) and Holstein × Gyr (HG; n = 7). Heifers grazed a consortium of ryegrass and bristle oats and were supplemented individually daily with cornmeal at 0.33% of body weight plus 5 kg/day of corn silage. For 3 consecutive days, feeding behavior was observed for individual animals from direct visual observation recording at 10-min intervals. The digestibility trial was performed on day (d) 16 to d24 of each period. Body measurements and weight were taken at d0 and at d23, 24, and 25 of each period. Grazing duration, grazing frequency, and bite rate were greater for HOL than those for HG. Rumination characteristics, intake, digestibility, and body measurements were not affected by breed. Breeds had differences in grazing characteristics, but they did not influence performance or intake parameters. Therefore, HOL and HG heifers managed under temperate pasture in tropical countries have similar performances.

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We aimed to evaluate the effects of two brands of bST (bovine somatoropin) on productive performance and on Minas Padrão cheese yield. Fifteen Girolando cows with more than 60 days of lactation were used. The animals were distributed in a randomized block design, according to days in milk, with three treatments (somatotropin A, somatotropin B, and control). The animals received a balanced diet with commercial concentrate and corn silage as roughage. It was measured milk yield and composition, dry matter intake, body condition score (BCS), and feeding behavior; it was compared by Tukey at 5.0% of significance level. Physical-chemical composition of Minas Padrão cheese was obtained from the different treatments, was also measured, being evaluated in a completely randomized design, and compared by Tukey at 1.0% of significance level. The dry matter intake per body weight lower in control treatment (p < 0.05) was equal to 2.85, somatotropin A was equal to 3.17, and somatotropin B was equal to 3.17. Animals that received bST showed higher milk production (p < 0.05), with control being 17.56 kg/day, somatotropin A 21.12 kg/day, and somatotropin B 20.69 kg/day. The physical-chemical composition of milk showed significant differences (p < 0.05) for the fat, protein, and dry extract contents. The somatotropin applications also influenced the BCS (p < 0.05) and the feeding behavior in time for total intake, time spent with rumination, and idleness. The application of bST increased milk yield and technical and economic yields of Minas Padrão cheese did not show significant differences between treatments.

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O rebanho Girolando tem crescido substancialmente nas últimas décadas no Brasil, tornando a raça uma das mais selecionadas e visadas para a exploração de leite entre os criadores. No entanto, é sabido a importância do manejo das fêmeas para evitar perdas durante a gestação, sendo elevado os abortos e as distocias no periparto, por desconhecimento da previsão de partos. Mas para estabelecer uma tabela de previsão de partos é necessário conhecer o período de gestação de raça explorada. Neste contexto, este estudo envolveu observações de três décadas (1990 a 2019) de avaliação dos efeitos de alguns fatores (ano de parto, sexo da cria, grupo sanguíneo materno, raça paterna e ordem de parto) sobre os períodos de gestação (PG) do rebanho Girolando do Centro Estadual de Pesquisa em Agricultura Orgânica (CEPAO), da Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (Pesagro-Rio). Para isso, foram utilizados os dados dos registros do rebanho dos sistemas de fichários e software Gera leite® , relativos aos PG (dia) de vacas Girolando, segundo as variáveis (1) ano, (2) sexo, (3) grupo sanguíneo materno, (4) raça paterno e (5) ordem de parto, tabulados e submetidos a análises descritivas, a anova one-way e teste Bonferroni no software Bioestat. Os métodos reprodutivos utilizados foram as inseminações, realizadas com a observação do estro, seguindo o sistema tradicional de Trimberg e, na última década, também a inseminação artificial em tempo fixo (IATF), com a utilização de protocolos hormonais para sincronização da ovulação. Os resultados médios, da avaliação de 2.104 partos não mostraram diferenças significativas (p>0,05), nos efeitos dos fatores analisados sobre os períodos de gestação. Assim, a média geral de 283,2±6,5 dias, pode ser considerada como uma estimativa dos partos esperados para o rebanho Girolando da Pesagro, a fim de proporcionar manejo e assistência adequados durante o parto, evitando distocias e perdas de mortalidade no periparto.

The Girolando herd has grown substantially in recent decades in Brazil, making the breed one of the most selected and targeted for milk exploration among breeders. However, it is known the importance of handling females to avoid losses during pregnancy, with abortions and dystocia in the peri-parturition, being high due to the lack of birth prediction. However, to establish a birth prediction table, it is necessary to know the gestation period of the exploited race. In this context, this study involved observations from three decades (1990 to 2019) to assess the effects of some factors (year of birth, sex of offspring, maternal blood group, paternal race and birth order), on gestation periods (PG) of the Girolando herd of the Centro Estadual de Pesquisa Agricultura Orgânica (CEPAO) of Pesagro-Rio. It is located in the municipality of Seropédica, RJ, in the Baixada Fluminense region, where in general the climate presents high temperatures (maximum ± 30oC) and relative air humidity (maximum 0.05), in the effects of the analyzed factors, on the gestation periods. Thus, the general average of 283.2±6.5 days can be considered as an estimate of expected deliveries for the Pesagro Girolando herd, in order to provide adequate management and assistance during childbirth, avoid dystocias and mortality losses in the peripartum.

Assuntos

RESUMO

O rebanho Girolando tem crescido substancialmente nas últimas décadas no Brasil, tornando a raça uma das mais selecionadas e visadas para a exploração de leite entre os criadores. No entanto, é sabido a importância do manejo das fêmeas para evitar perdas durante a gestação, sendo elevado os abortos e as distocias no periparto, por desconhecimento da previsão de partos. Mas para estabelecer uma tabela de previsão de partos é necessário conhecer o período de gestação de raça explorada. Neste contexto, este estudo envolveu observações de três décadas (1990 a 2019) de avaliação dos efeitos de alguns fatores (ano de parto, sexo da cria, grupo sanguíneo materno, raça paterna e ordem de parto) sobre os períodos de gestação (PG) do rebanho Girolando do Centro Estadual de Pesquisa em Agricultura Orgânica (CEPAO), da Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (Pesagro-Rio). Para isso, foram utilizados os dados dos registros do rebanho dos sistemas de fichários e software Gera leite® , relativos aos PG (dia) de vacas Girolando, segundo as variáveis (1) ano, (2) sexo, (3) grupo sanguíneo materno, (4) raça paterno e (5) ordem de parto, tabulados e submetidos a análises descritivas, a anova one-way e teste Bonferroni no software Bioestat. Os métodos reprodutivos utilizados foram as inseminações, realizadas com a observação do estro, seguindo o sistema tradicional de Trimberg e, na última década, também a inseminação artificial em tempo fixo (IATF), com a utilização de protocolos hormonais para sincronização da ovulação. Os resultados médios, da avaliação de 2.104 partos não mostraram diferenças significativas (p>0,05), nos efeitos dos fatores analisados sobre os períodos de gestação. Assim, a média geral de 283,2±6,5 dias, pode ser considerada como uma estimativa dos partos esperados para o rebanho Girolando da Pesagro, a fim de proporcionar manejo e assistência adequados durante o parto, evitando distocias e perdas de mortalidade no periparto.(AU)

The Girolando herd has grown substantially in recent decades in Brazil, making the breed one of the most selected and targeted for milk exploration among breeders. However, it is known the importance of handling females to avoid losses during pregnancy, with abortions and dystocia in the peri-parturition, being high due to the lack of birth prediction. However, to establish a birth prediction table, it is necessary to know the gestation period of the exploited race. In this context, this study involved observations from three decades (1990 to 2019) to assess the effects of some factors (year of birth, sex of offspring, maternal blood group, paternal race and birth order), on gestation periods (PG) of the Girolando herd of the Centro Estadual de Pesquisa Agricultura Orgânica (CEPAO) of Pesagro-Rio. It is located in the municipality of Seropédica, RJ, in the Baixada Fluminense region, where in general the climate presents high temperatures (maximum ± 30oC) and relative air humidity (maximum < 60%). For this, data from the herd records of the binder systems and Gera leite® software were used, related to the PG (day) of Girolando cows, according to (1) year, (2) sex, (3) maternal blood group, (4) paternal race and (5) birth order were tabulated and submitted to descriptive analyzes, the one-way anova and Bonferroni test in the Bioestat software. The reproductive methods used were inseminations, performed with the observation of estrus, following the traditional Trimberg system and, in the last decade, also artificial insemination in fixed time (TAI), with the use of hormonal protocols for synchronization of ovulation). The results of the evaluation did not show significant differences (p>0.05), in the effects of the analyzed factors, on the gestation periods. Thus, the general average of 283.2±6.5 days can be considered as an estimate of expected deliveries for the Pesagro Girolando herd, in order to provide adequate management and assistance during childbirth, avoid dystocias and mortality losses in the peripartum.(AU)

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RESUMO

A biópsia embrionária associada à genotipagem permite a obtenção de informações genômicas antes mesmo da transferência dos embriões. Neste estudo, foram avaliadas amostras biopsiadas de blastocistos bovinos transferidos para receptoras (n=47), sob a hipótese de que a raça (Gir ou Girolando), o estádio embrionário (blastocisto ou blastocisto expandido) e a competência para estabelecimento de prenhez (positiva ou negativa) afetariam a quantidade e a qualidade do DNA da amostra obtida. O DNA foi extraído, amplificado, quantificado por eletroferograma e genotipado. O parâmetro call rate (CR) foi adotado para mensurar a qualidade da genotipagem. Obteve-se concentração de DNA de 86,07±171,66ng/µL e CR 0,73±0,17. O CR não variou em função da quantidade de DNA nas amostras. As variáveis raça e estádio embrionário não influenciaram a concentração de DNA, nem o CR. Houve efeito da prenhez sobre o CR (P=0,0187), mas, como houve maior CR nas amostras provenientes do grupo prenhez negativa, não foi possível associar esse parâmetro à qualidade embrionária. Concluiu-se que a raça e a qualidade embrionária não afetam os parâmetros aqui estudados em amostras embrionárias, ou seja, embriões com maiores chances de implantação não refletem alta qualidade nas amostras de biópsia genotipadas.(AU)

Embryo biopsy associated with genotyping allows genomic information before embryo transfer. In this study, blastocyst biopsy samples from embryos transferred to recipients (n= 47) were evaluated, under the hypothesis that breed (Gyr or Girolando), embryonic stage (blastocyst or expanded blastocyst) and competence to establish pregnancy (positive or negative) would affect the quantity and DNA quality of samples. DNA was extracted, amplified, quantified by electropherogram and genotyped. The parameter call rate (CR) was used to measure the quality of genotyping. DNA concentration of 86.07±171.66ng/µl, and CR 0.73±0.17 was obtained. CR did not vary according to the amount of DNA in the samples. The variables breed and embryonic stage had no influence on DNA concentration or CR. There was pregnancy effect on the CR (P= 0.0187), but since there was a higher CR in the samples from the negative pregnancy group, it was not possible to associate this parameter with the embryonic quality. We conclude that the breed and embryo quality do not affect the evaluated parameters in embryonic samples. Embryos with higher chances of implantation do not reflect high quality in embryo biopsy genotyped samples.(AU)

Assuntos

RESUMO

A biópsia embrionária associada à genotipagem permite a obtenção de informações genômicas antes mesmo da transferência dos embriões. Neste estudo, foram avaliadas amostras biopsiadas de blastocistos bovinos transferidos para receptoras (n=47), sob a hipótese de que a raça (Gir ou Girolando), o estádio embrionário (blastocisto ou blastocisto expandido) e a competência para estabelecimento de prenhez (positiva ou negativa) afetariam a quantidade e a qualidade do DNA da amostra obtida. O DNA foi extraído, amplificado, quantificado por eletroferograma e genotipado. O parâmetro call rate (CR) foi adotado para mensurar a qualidade da genotipagem. Obteve-se concentração de DNA de 86,07±171,66ng/µL e CR 0,73±0,17. O CR não variou em função da quantidade de DNA nas amostras. As variáveis raça e estádio embrionário não influenciaram a concentração de DNA, nem o CR. Houve efeito da prenhez sobre o CR (P=0,0187), mas, como houve maior CR nas amostras provenientes do grupo prenhez negativa, não foi possível associar esse parâmetro à qualidade embrionária. Concluiu-se que a raça e a qualidade embrionária não afetam os parâmetros aqui estudados em amostras embrionárias, ou seja, embriões com maiores chances de implantação não refletem alta qualidade nas amostras de biópsia genotipadas.(AU)

Embryo biopsy associated with genotyping allows genomic information before embryo transfer. In this study, blastocyst biopsy samples from embryos transferred to recipients (n= 47) were evaluated, under the hypothesis that breed (Gyr or Girolando), embryonic stage (blastocyst or expanded blastocyst) and competence to establish pregnancy (positive or negative) would affect the quantity and DNA quality of samples. DNA was extracted, amplified, quantified by electropherogram and genotyped. The parameter call rate (CR) was used to measure the quality of genotyping. DNA concentration of 86.07±171.66ng/µl, and CR 0.73±0.17 was obtained. CR did not vary according to the amount of DNA in the samples. The variables breed and embryonic stage had no influence on DNA concentration or CR. There was pregnancy effect on the CR (P= 0.0187), but since there was a higher CR in the samples from the negative pregnancy group, it was not possible to associate this parameter with the embryonic quality. We conclude that the breed and embryo quality do not affect the evaluated parameters in embryonic samples. Embryos with higher chances of implantation do not reflect high quality in embryo biopsy genotyped samples.(AU)

Assuntos

RESUMO

The objective of this study was to evaluate the production, consumption, and energy balance parameters of primiparous 3/4 and 7/8 Holstein × Gir (HG) dairy cows fed two diets of differing energy levels during the postpartum period. At the beginning of the study, 28 days prepartum, the average weight of both genetic groups was 498 ± 12 kg and body condition score (BCS) was 3.5 ± 0.05. At the end of the study, 61 days postpartum, the 3/4 HG cows had higher weight and body condition scores than the 7/8 HG (456 ± 8 and 429 ± 8 kg and 3.13 ± 0.03 and 2.94 ± 0.03 BCS for 3/4 HG and 7/8 HG, respectively). Milk from cows fed the high-energy diet had higher percentages of fat, protein, lactose, and total dry extract than cows fed the low-energy diet. Cows fed the high-energy diet had higher net energy intake (95.3 ± 1.9 vs. 88.1 ± 2.1 MJ/day) and higher energy balance (3.64 ± 2.13 vs - 6.02 ± 2.30 MJ/day). The 3/4 HG cows displayed higher energy for maintenance (33.1 ± 0.4 MJ/day) than the 7/8 HG (31.5 ± 0.5 MJ /day). In conclusion, although the primiparous 3/4 HG were heavier than the 7/8 HG and had a higher body condition score, no differences in milk produced up to 60 days postpartum were observed. The higher energy diet during the postpartum period increased energy balance, resulting in higher production of milk fat, protein, and lactose.

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Holstein-Gyr crossbred cattle are strategic for dairy systems in tropical countries, since they combine milk yield genetics with adaptability to tropical climate. However, Holstein (Bos taurus) and Gyr (Bos indicus) breeds present remarkable differences regarding reproductive physiology. Brazil stands out as the worlds largest user of embryo in vitro production (IVP) in bovine, and the use of this technique is increasing in dairy systems. As Holstein-Gyr crossbreds are important oocyte donors for IVP, the present work aimed at investigating whether increased Gyr or Holstein breed composition influences donors performance. Sixteen Holstein-Gyr crossbred females presenting increased (HG, 71.4 to 87.5% Holstein; n = 9) or decreased (GH, 40.2 to 46.6% Holstein; n = 7) Holstein composition were submitted to three ovum pick up (OPU) sessions. We observed similar (P = 0.2946) antral follicle count between HG and GH donors (24.8 ± 3.2 vs 29.4 ± 2.8 respectively; mean ± SEM). Groups also display similar morphological oocyte grading (Grade I: 0.1 ± 0.1 vs 0.1 ± 0.1 P = 0.9680; Grade II: 0.9 ± 0.5 vs 1.9 ± 0.5 P = 0.1942; Grade III, 4.0 ± 1.2 vs 7.2 ± 1.4 P = 0.1047, HG vs GH respectively; mean ± SEM). Additionally, the proportion of viable oocyte was similar between HG and GH groups (27.8% vs 31.9%, respectively, P = 0.3500) and oocyte lipid area fraction (6.8% vs 9.5%, respectively; P = 0.1539). Our results indicate that the individual variation has more influence than breed composition of crossbred oocyte donors.(AU)

Assuntos

RESUMO

Holstein-Gyr crossbred cattle are strategic for dairy systems in tropical countries, since they combine milk yield genetics with adaptability to tropical climate. However, Holstein (Bos taurus) and Gyr (Bos indicus) breeds present remarkable differences regarding reproductive physiology. Brazil stands out as the world’s largest user of embryo in vitro production (IVP) in bovine, and the use of this technique is increasing in dairy systems. As Holstein-Gyr crossbreds are important oocyte donors for IVP, the present work aimed at investigating whether increased Gyr or Holstein breed composition influences donor’s performance. Sixteen Holstein-Gyr crossbred females presenting increased (HG, 71.4 to 87.5% Holstein; n = 9) or decreased (GH, 40.2 to 46.6% Holstein; n = 7) Holstein composition were submitted to three ovum pick up (OPU) sessions. We observed similar (P = 0.2946) antral follicle count between HG and GH donors (24.8 ± 3.2 vs 29.4 ± 2.8 respectively; mean ± SEM). Groups also display similar morphological oocyte grading (Grade I: 0.1 ± 0.1 vs 0.1 ± 0.1 – P = 0.9680; Grade II: 0.9 ± 0.5 vs 1.9 ± 0.5 – P = 0.1942; Grade III, 4.0 ± 1.2 vs 7.2 ± 1.4 – P = 0.1047, HG vs GH respectively; mean ± SEM). Additionally, the proportion of viable oocyte was similar between HG and GH groups (27.8% vs 31.9%, respectively, P = 0.3500) and oocyte lipid area fraction (6.8% vs 9.5%, respectively; P = 0.1539). Our results indicate that the individual variation has more influence than breed composition of crossbred oocyte donors.

Assuntos

RESUMO

Holstein-Gyr crossbred cattle are strategic for dairy systems in tropical countries, since they combine milk yield genetics with adaptability to tropical climate. However, Holstein (Bos taurus) and Gyr (Bos indicus) breeds present remarkable differences regarding reproductive physiology. Brazil stands out as the world's largest user of embryo in vitro production (IVP) in bovine, and the use of this technique is increasing in dairy systems. As Holstein-Gyr crossbreds are important oocyte donors for IVP, the present work aimed at investigating whether increased Gyr or Holstein breed composition influences donor's performance. Sixteen Holstein-Gyr crossbred females presenting increased (HG, 71.4 to 87.5% Holstein; n = 9) or decreased (GH, 40.2 to 46.6% Holstein; n = 7) Holstein composition were submitted to three ovum pick up (OPU) sessions. We observed similar (P = 0.2946) antral follicle count between HG and GH donors (24.8 ± 3.2 vs 29.4 ± 2.8 respectively; mean ± SEM). Groups also display similar morphological oocyte grading (Grade I: 0.1 ± 0.1 vs 0.1 ± 0.1 - P = 0.9680; Grade II: 0.9 ± 0.5 vs 1.9 ± 0.5 - P = 0.1942; Grade III, 4.0 ± 1.2 vs 7.2 ± 1.4 - P = 0.1047, HG vs GH respectively; mean ± SEM). Additionally, the proportion of viable oocyte was similar between HG and GH groups (27.8% vs 31.9%, respectively, P = 0.3500) and oocyte lipid area fraction (6.8% vs 9.5%, respectively; P = 0.1539). Our results indicate that the individual variation has more influence than breed composition of crossbred oocyte donors.

RESUMO

We report an unusual case of obstructive fibrosis of the venous ring of Fürstenberg in all four quarters of the udder of a cow of the Girolando breed (3/8 Gir and 5/8 Dutch). The information was obtained through a review of medical records, anamnesis, and imaging methods such as theloscopy and ultrasonography. Histopathological analysis provided a definitive diagnosis.(AU)

Relata-se a ocorrência de um caso incomum de fibrose obstrutiva do anel venoso de Fürstenberg em quatro quartos mamários de uma vaca da raça Girolando (3/8 Gir e 5/8 Holandês). As informações foram obtidas por meio de revisão do prontuário, da anamnese e de métodos de diagnóstico por imagem, como a teloscopia e a ultrassonografia. A análise histopatológica forneceu o diagnóstico definitivo.(AU)

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RESUMO

We report an unusual case of obstructive fibrosis of the venous ring of Fürstenberg in all four quarters of the udder of a cow of the Girolando breed (3/8 Gir and 5/8 Dutch). The information was obtained through a review of medical records, anamnesis, and imaging methods such as theloscopy and ultrasonography. Histopathological analysis provided a definitive diagnosis.(AU)

Relata-se a ocorrência de um caso incomum de fibrose obstrutiva do anel venoso de Fürstenberg em quatro quartos mamários de uma vaca da raça Girolando (3/8 Gir e 5/8 Holandês). As informações foram obtidas por meio de revisão do prontuário, da anamnese e de métodos de diagnóstico por imagem, como a teloscopia e a ultrassonografia. A análise histopatológica forneceu o diagnóstico definitivo.(AU)

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RESUMO

This study had the objective of evaluate the quality and the proportion of COCs collected by follicularaspiration Girolanda females. Eight cows and ten heifers were submitted in four OPU sessions with an intervalof 15 days. The heifer category provides a larger amount of recovered structures (GIII and AT / DG) comparedto cows, but cows had higher amount of GII.

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RESUMO

This study evaluated the efficacy of a long term progesterone protocols associated with PGF2a andGnRH by measurement of follicular diameter (FD) in D14, D22 and D30; time between the end of treatment andthe onset of estrus and conception rate in groups of Girolando heifers and Girolando cows (N1 and N2, V1 andV2). On D30, the diameter of FD for the groups V1, V2, N1 and N2 was 13.3 ± 0,11mm, 13.8 ± 0.14 mm, 0.10mm and 13.7 ± 12.7 ± 0.49mm respectively. The time between the end of the treatment and the onset of estrus forthe groups V1, V2, and N1, N2 was 115,2hs, 103,2hs, 127,2hs, 129,6hs, respectively. Based on the results it canbe concluded that the protocols were efficient for obtaining a dominant follicle in the PGF2a application dayfavoring a good conception rate.

Assuntos

RESUMO

This study had the objective of evaluate the quality and the proportion of COCs collected by follicularaspiration Girolanda females. Eight cows and ten heifers were submitted in four OPU sessions with an intervalof 15 days. The heifer category provides a larger amount of recovered structures (GIII and AT / DG) comparedto cows, but cows had higher amount of GII.(AU)

Assuntos

RESUMO

This study evaluated the efficacy of a long term progesterone protocols associated with PGF2a andGnRH by measurement of follicular diameter (FD) in D14, D22 and D30; time between the end of treatment andthe onset of estrus and conception rate in groups of Girolando heifers and Girolando cows (N1 and N2, V1 andV2). On D30, the diameter of FD for the groups V1, V2, N1 and N2 was 13.3 ± 0,11mm, 13.8 ± 0.14 mm, 0.10mm and 13.7 ± 12.7 ± 0.49mm respectively. The time between the end of the treatment and the onset of estrus forthe groups V1, V2, and N1, N2 was 115,2hs, 103,2hs, 127,2hs, 129,6hs, respectively. Based on the results it canbe concluded that the protocols were efficient for obtaining a dominant follicle in the PGF2a application dayfavoring a good conception rate.(AU)

Assuntos

RESUMO

O Brasil atualmente é detentor do terceiro maior rebanho de vacas leiteiras do mundo, composto, em sua maioria, de animais mestiços F1 (Gir X Holandês), os quais são bem adaptados às áreas tropicais. O objetivo do presente estudo foi investigar a eficiência da ovum pick-up (OPU) e da produção in vitro de embriões oriundos de doadoras Girolando com sêmen sexado de touros provenientes de duas raças, Gir e Holandesa. Foram utilizados dados referentes a 232 animais de diversos rebanhos, totalizando 4334 oócitos recuperados. Os oócitos foram classificados, e as estruturas viáveis (GI, GII e GIII) foram utilizadas para produção in vitro de embriões (PIVE). Foi observada uma média de oócitos recuperados para as doadoras F1 de 18,14±1,33. Além disso, notou-se que não houve diferença entre os dois cruzamentos utilizados, considerando média de total de embriões (4,96±0,40 e 6,56±0,76) para o cruzamento F1 X HPB (Holandês preto e branco) e F1 X Gir, respectivamente. Portanto, as doadoras F1 apresentaram potencial como doadoras de oócitos no sistema de produção in vitro de embriões, independentemente da raça do touro cujo sêmen foi utilizado.(AU)

Brazil currently has the third largest herd of dairy cattle in the world, composed mainly by F1 crossbreed animals (Gyr x Holstein), which are well adapted to tropical areas. The aim of this study was to evaluate the efficiency of Girolando donors in OPU and in vitro production of embryos (IVEP) using sexed semen of bulls from two breeds, Gyr and Holstein. We used data from 232 animals coming from different herds and the total of recovered oocytes was 4334. The oocytes were classified according to their viability and the viable ones (GI, GII and GIII) were used for IVP. The average of oocyte recovery from F1 donors was 18.14±1.33. There was no difference on the average of embryos considering the crossing F1 X Holstein (4.96±0.40) and F1 X Gyr (6.56±0.76). In conclusion, F1 donors showed a potential for being oocyte donors in the system of in vitro production of embryos, regardless the breed of the bull.(AU)

Assuntos

RESUMO

O Brasil atualmente é detentor do terceiro maior rebanho de vacas leiteiras do mundo, composto, em sua maioria, de animais mestiços F1 (Gir X Holandês), os quais são bem adaptados às áreas tropicais. O objetivo do presente estudo foi investigar a eficiência da ovum pick-up (OPU) e da produção in vitro de embriões oriundos de doadoras Girolando com sêmen sexado de touros provenientes de duas raças, Gir e Holandesa. Foram utilizados dados referentes a 232 animais de diversos rebanhos, totalizando 4334 oócitos recuperados. Os oócitos foram classificados, e as estruturas viáveis (GI, GII e GIII) foram utilizadas para produção in vitro de embriões (PIVE). Foi observada uma média de oócitos recuperados para as doadoras F1 de 18,14±1,33. Além disso, notou-se que não houve diferença entre os dois cruzamentos utilizados, considerando média de total de embriões (4,96±0,40 e 6,56±0,76) para o cruzamento F1 X HPB (Holandês preto e branco) e F1 X Gir, respectivamente. Portanto, as doadoras F1 apresentaram potencial como doadoras de oócitos no sistema de produção in vitro de embriões, independentemente da raça do touro cujo sêmen foi utilizado.(AU)

Brazil currently has the third largest herd of dairy cattle in the world, composed mainly by F1 crossbreed animals (Gyr x Holstein), which are well adapted to tropical areas. The aim of this study was to evaluate the efficiency of Girolando donors in OPU and in vitro production of embryos (IVEP) using sexed semen of bulls from two breeds, Gyr and Holstein. We used data from 232 animals coming from different herds and the total of recovered oocytes was 4334. The oocytes were classified according to their viability and the viable ones (GI, GII and GIII) were used for IVP. The average of oocyte recovery from F1 donors was 18.14±1.33. There was no difference on the average of embryos considering the crossing F1 X Holstein (4.96±0.40) and F1 X Gyr (6.56±0.76). In conclusion, F1 donors showed a potential for being oocyte donors in the system of in vitro production of embryos, regardless the breed of the bull.(AU)

Assuntos