RESUMO
RESUMEN Introducción: Los teratomas se definen como tumores de tejidos extraños al órgano o sitio anatómico en el cual se originan. Los teratomas mediastinales no son frecuentes, representan alrededor del 5 por ciento al 10 por ciento de todos los tumores mediastinales. Objetivo: Describir los resultados del tratamiento quirúrgico de pacientes con diagnóstico de teratomas mediastinales. Métodos: Se estudiaron 12 pacientes tratados entre enero de 2001 y diciembre de 2018. Las variables evaluadas fueron sexo, edad, tipo histológico: maduro o inmaduro, vía de acceso quirúrgico, accidentes quirúrgicos y evolución postoperatoria. Resultados: Correspondieron 9 al sexo femenino y 3 al masculino. El promedio de edad fue de 33,3 años (17-60 años). Hubo predominio absoluto del tipo maduro (11). La esternotomía media fue el acceso más frecuente. La toracotomía se realizó cuando el tumor, voluminoso, ocupaba la mayor parte de un hemitórax. Los accidentes quirúrgicos fueron un desgarro pulmonar y una apertura del pericardio. De dos pacientes tratados mediante cirugía torácica videoasistida, uno fue convertido por sangrado venoso molesto. Al año de seguimiento todos estaban vivos, sin evidencias de recidiva. Conclusiones: Contrariamente a lo esperado, hay predominio del sexo femenino, mientras que la edad y el tipo histológico coinciden con la literatura. La esternotomía, aún hoy, es comúnmente aceptada, a pesar del auge de la cirugía torácica videoasistida. La resección total produce resultados excelentes para los teratomas benignos(AU)
ABSTRACT Introduction: Teratomas are defined as tumors of tissues foreign to the organ or anatomical site in which they originate. Mediastinal teratomas are rare, accounting for about 5-10 percent of all mediastinal tumors. Objective: To describe the outcomes of the surgical treatment of patients diagnosed with mediastinal teratomas. Methods: Twelve patients treated between January 2001 and December 2018 were studied. The variables evaluated were sex, age, histological type (mature or immature), surgical access route, surgical accidents, and postoperative evolution. Results: Nine patients corresponded to the female sex and three, to the male. The average age was 33.3 years (17-60 years). There was an absolute predominance of the mature type (11). Median sternotomy was the most frequent access. Thoracotomy was performed when the bulky tumor occupied most of a hemithorax. The surgical accidents were lung tear and opening of the pericardium. Of two patients treated by video-assisted thoracic surgery, one was converted for bothersome venous bleeding. At one year of follow-up, all were alive, with no evidence of recurrence. Conclusions: Contrary to expectations, there is predominance of the female sex, while age and histological type coincide with the literature. Sternotomy, even today, is commonly accepted, despite the rise of video-assisted thoracic surgery. Total resection produces excellent outcomes in benign teratomas(AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Teratoma/diagnóstico , Toracotomia/métodos , Cirurgia Torácica Vídeoassistida/métodos , Esternotomia/métodos , Estudos RetrospectivosRESUMO
In vitro culture of parasitic helminths provides an important tool to study cell regeneration and physiology, as well as for molecular biology and genetic engineering studies. In the present study, we established in vitro propagation of cells from Echinococcus granulosus germinal cyst layer. E. granulosus germinal cells grew beyond 100 passages and showed no signs of reduced proliferation capacity. Microscopic analysis revealed that cells grew both attached to the substrate and in suspension, forming three-dimensional structures like mammalian stem cell aggregates. Examination of the chromosome number of attached germinal cells showed a high degree of heteroploidy, suggesting the occurrence of transformation during culture. Monolayer cells survived cryopreservation and were able to proliferate after thawing. Based on the characteristics displayed by E. granulosus germinal cells, we establish a cell line from the E. granulosus germinal layer. Furthermore, we propose that this cell line could be useful for drug screening and for obtaining parasite material.
Assuntos
Linhagem Celular , Echinococcus granulosus/citologia , Células Germinativas/crescimento & desenvolvimento , Parasitologia/métodos , Animais , Técnicas de Cultura de Células/métodos , Proliferação de Células , Criopreservação/métodos , MicroscopiaRESUMO
Se relacionó la exposición ocupacional a agroquímicos con parámetros seminales vinculados con la espermatogénesis. En 104 muestras seminales de pacientes con infertilidad idiopática y 20 a 45 años de edad del Servicio de Reproducción Hospital Centenario (Rosario, Argentina), se efectuó espermograma y estudios funcionales según normas OMS (1999). Se agruparon en G1 (n=42) trabajadores expuestos a agroquímicos y G2 (n=62) hombres sin riesgo espermatogénico. La concentración espermática se determinó en cámara de Neubauer, con Papanicolaou se evaluó la concentración de células germinales y la morfología espermática. Se observó en concentración espermática (espermatozoides.10(6)/ml) G1: 21,1±7,1; G2: 41,6±9,2; morfología ( por ciento espermatozoides normales) G1: 5,2±1,3; G2: 8,2±3,6 y células germinales.10(6)/ml en G1: 0,91±0,51; G2: 0,32±0,21. La concentración espermática fue menor en G1, las alteraciones morfológicas y células germinales mayores en G1. Estos resultados indican que la exposición a agroquímicos altera la espermatogénesis y es un factor a considerar cuando se estudia infertilidad masculina.
The occupational exposition to agrochemicals was related with sperm parameters linked with spermatogenesis. In 104 semen samples of patients with idiopatic infertility of the Reproduction Service of Centenario Hospital (Rosario, Argentina), sperm study and functional tests according to WHO (1999) have been carried out. Two groups were formed: G1 (n=42) workers exposed to agrochemicals; G2 (n=62) men with no spermatogenic risk. The sperm concentration was determined in Neubauer camera, the concentration of germinal cells and the sperm morphology were evaluated with Papanicolaou. The results were: sperm concentration (spermatozoids 10(6)/ml) G1: 21.1 ± 7.1; G2: 41.6 ± 9.2; morphology ( percent normal spermatozoids) G1: 5.2 ± 1.3; G2: 8.2 ± 3.6; and germinal cells 10(6)/ml in G1: 0.91 ± 0.51; G2: 0.32 ± 0.21. The sperm concentration was lower in G1, major morphological changes and germinal cells in G1. This results show that the exposition to agrochemicals alters the spermatogenesis, it is a factor to consider in the male infertility.
RESUMO
Los teratomas son tumores, que presentan en su estructura tejidos derivados de las tres capas germinales (ectodermo, endodermo y mesodermo); como pelos, tejido cerebral, uñas, etc. Pueden ser benignos o malignos. El teratoma quístico maduro, es el tumor de células germinales más frecuente, y afecta principalmente a mujeres jóvenes. El caso reportado es el de una mujer de 21 años, que fue diagnosticada de teratoma quístico maduro en ambos ovarios. Para la extirpación se prefirió cirugía abierta por sobre la laparoscópica, por el tamaño de las masas.
Teratomas are tumors that present in their structure, derived tissues from the three germinal layers (Ectoderm, Mesoderm, and Endoderm); as hair, cerebral tissue, nails, etc. They can be benign or malignant. Mature cystic teratoma (benign dermoyd cyst), is the most frequent germ cells tumor which principally affects young woman. The case reported is of a 21 years old woman who was diagnosed of mature cystic teratoma in both ovaries. For extirpation, open surgery was preferred over laparoscopic by the size of the mass.
Assuntos
Adulto , Feminino , Adulto Jovem , Neoplasias Embrionárias de Células Germinativas , Neoplasias Ovarianas , Ovário , TeratomaRESUMO
Este estudo teve como objetivo acompanhar o processo de desenvolvimento testicular desde a fase indiferenciada até sua completa formação. Embriões e fetos de vacas da raça nelore (Bos taurus indicus) foram obtidos em frigoríficos próximos à cidade de Uberlândia, Minas Gerais. As gônadas dos fetos e os embriões foram fixados em bouin e processados para microscópica óptica convencional. A gônada foi observada primeiramente em um embrião de 1,0 cm de comprimento. Em embriões com 2,5 cm a presença da albugínea permite a identificação do sexo. A espessura média da albugínea variou de 29,08 a 558,46 mm. Gradativamente, observou-se aumento da vascularização da albugínea e do parênquima. O mediastino encontrava-se localizado centralmente. Houve uma diminuição no espaço ocupado pelos cordões testiculares de 63,71 para 41,99% do volume total dos testículos. O seu diâmetro variou de 31,68 a 48,80 mm. O diâmetro das células germinativas (e dos seus núcleos) foi de 12,27(6,65) a 16,95 (14,21) mm, respectivamente. A quantidade de células germinativas por corte transversal de cordão diminuiu de um máximo de 2,80 para 0,76. O número total de células germinativas foi de 16 no princípio da colonização da gônada para 18,32 x 106 no final do estudo. O número de células de Sertoli por corte transversal de cordão variou de 10,00 a 16,25. Os resultados obtidos mostram que a origem e a formação dos testículos nos embriões e fetos de vacas da raça Nelore (Bos taurus indicus) ocorre de forma muito semelhante ao do que é descrito para Bos taurus taurus.(AU)
The aim of this study was to accompany the process of testicular development from the non-differentiable phase to its complete formation. Embryos and fetuses of Nelore breed cows (Bos Taurus indicus) were obtained in slaughterhouses near the Uberlandia city, Minas Gerais. The gonads and the embryos were fixed in Bouins fixative and afterwards processed for conventional optical microscopy. The gonadal was observed firstly in a 1.0 cm long embryo. In 2.5 cm long embryos the presence of the albuginea allows the sex identification. The mean thickness of the albuginea ranged from 29.08 to 558.45 mm. Gradually increase of vascularization of the albuginea and parenchyma is observed. The mediastinum is located centrally. There was a decrease in the space occupied by the testicular cords, from 63.71 to 41.99% of the total testes volume. Its diameter ranged from 31.68 to 48.80 mm. The diameter of germinal cells (and their nuclei) was from 12.27 (6.65) to 16.95 914.21) mm. The quantity of germinal cells by cross section of cord decreased from a maximum of 2.80 to 0.76. The total number of germinal cells was from 16 at the beginning of colonization of the gonad to 18.32 x 106 at the end of the study. The number of Sertolis cells by cross section of cord ranged from 10.00 to 16.25. The results obtained show that the origin and formation of testes in embryos and fetuses from Nelore breed cows (Bos taurus indicus) does occur in a very similar way to what is described for Bos taurus taurus.(AU)